Composition for resisting cell senescence and application thereof

Abstract

The invention provides a composition for resisting cell aging and application thereof. The composition comprises the following components in parts by weight: 3-9 parts of a traditional Chinese medicine extract A, 2-8 parts of a traditional Chinese medicine extract B, 4-8 parts of a traditional Chinese medicine extract C and 5-11 parts of a traditional Chinese medicine extract D. Wherein the extraction raw material of the traditional Chinese medicine extract A is centella asiatica; the extraction raw material of the traditional Chinese medicine extract B is ginseng; the extraction raw materials of the traditional Chinese medicine extract C comprise Chinese prickly ash and ginger roots; the extraction raw materials of the traditional Chinese medicine extract D comprise gentiana macrophylla and gentian. The four traditional Chinese medicine extracts A-D are screened and are compatible according to a specific proportion, so that cell proliferation can be synergistically promoted, the oxidation resistance can be synergistically improved, MMP-1 is inhibited to promote collagen expression, and the anti-aging effect is achieved.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicine, and in particular relates to an anti-cell aging composition and application thereof. Background Art

[0002] As people age, their skin typically shows signs of aging. This is especially true in everyday life, where external factors such as ultraviolet radiation and blue light from electronic devices can further exacerbate skin aging. In recent years, with the improvement of people's living standards and the increasing pursuit of beauty, a variety of anti-aging chemical drugs and health supplements have emerged. However, their effectiveness varies greatly, and some can even have adverse effects. Compared to oral chemical drugs or health supplements, traditional Chinese medicine extracts contain a variety of active ingredients with antioxidant, immune-enhancing, metabolic, and endocrine-regulating effects. They have become a hot topic in anti-aging research.

[0003] However, at present, the antioxidant capacity and anti-aging effects of anti-cellular aging products with Chinese herbal medicine extracts as the main ingredients still need to be improved. Summary of the Invention

[0004] In view of this, the present invention aims to provide an anti-aging composition and its application. The anti-aging composition has excellent cell proliferation promoting effects, can significantly improve antioxidant capacity, inhibit MMP-1 and promote collagen expression, thereby achieving anti-aging effects.

[0005] To achieve this object, the present invention adopts the following technical solutions:

[0006] The present invention provides an anti-cell aging composition, comprising, by weight:

[0007]

[0008] The extraction raw material of the Chinese medicine extract A is Centella asiatica;

[0009] The extraction raw material of the Chinese medicine extract B is ginseng;

[0010] The extraction raw materials of the Chinese medicine extract C include Zanthoxylum bungeanum and ginger root;

[0011] The extraction raw materials of the traditional Chinese medicine extract D include Gentiana macrophylla and Gentiana scabra.

[0012] Preferably, the composition comprises, by weight:

[0013]

[0014] Preferably, the extraction raw materials of the traditional Chinese medicine extract C include 1 part of Zanthoxylum bungeanum and 1 to 3 parts of ginger root.

[0015] Preferably, the extraction raw materials of the traditional Chinese medicine extract D include 1 part of Gentiana macrophylla and 0.8-2 parts of Gentiana scabra.

[0016] Preferably, the Chinese herbal extract A is obtained by subjecting Centella asiatica to carbon dioxide extraction in the presence of an entrainer.

[0017] Preferably, the extraction conditions are set as follows: temperature is 50-80° C., and pressure is 15-30 MPa.

[0018] Preferably, the entrainer is 40-50 wt% ethanol.

[0019] Preferably, the extraction is performed 1 to 3 times, and the extraction further includes the steps of concentration and drying.

[0020] Preferably, the Chinese herbal extract B is obtained by ultrasonic extraction of a mixture of ginseng and a solvent.

[0021] Preferably, the solid-liquid ratio of the ginseng to the solvent is 1:20 to 1:35 g / mL.

[0022] Preferably, the solvent is 60-80 wt% ethanol.

[0023] Preferably, the extraction is performed 1 to 3 times, and the extraction further includes the steps of concentration and drying.

[0024] Preferably, the Chinese herbal extract C is obtained by extracting volatile oil from a mixture of Zanthoxylum bungeanum, ginger root and water using steam distillation and then drying the volatile oil.

[0025] Preferably, the ratio of the total mass of the pepper and ginger root to the mass of water is 1:(10-15).

[0026] Preferably, the Chinese herbal extract D is obtained by flash extraction of a mixture of Gentiana macrophylla, Gentiana scabra and a solvent.

[0027] Preferably, the extraction conditions are set as: extraction temperature 55-70° C., extraction voltage 110-120 V, and extraction time 60-120 s.

[0028] Preferably, the solvent is 40-60 wt% ethanol, and the ratio of the total mass of the Gentiana macrophylla and Gentiana scabra to the volume of the solvent is 1 g: (50-70) mL.

[0029] Preferably, the extraction is performed 1 to 3 times, and the extraction further includes the steps of concentration and drying.

[0030] In a second aspect, the present invention provides an anti-cellular aging product, comprising the above-mentioned anti-cellular aging composition.

[0031] Preferably, the product comprises a serum, a lotion or a cream.

[0032] Compared with the prior art, the present invention has the following beneficial effects:

[0033] The present invention provides an anti-cellular aging composition comprising, by weight, 3-9 parts of a Chinese herbal extract A, 2-8 parts of a Chinese herbal extract B, 4-8 parts of a Chinese herbal extract C, and 5-11 parts of a Chinese herbal extract D. The raw material for extract A is Centella asiatica; the raw material for extract B is Panax ginseng; the raw materials for extract C include Zanthoxylum bungeanum and ginger root; and the raw materials for extract D include Gentiana macrophylla and Gentiana scabra. The present invention screens the four Chinese herbal extracts A-D and combines them in specific proportions to synergistically promote cell proliferation, enhance antioxidant capacity, inhibit MMP-1, and promote collagen expression, thereby achieving an anti-aging effect. Compared to a formula containing any single component, or a formula in which any component is deleted or replaced, the composition comprising Chinese herbal extracts A-D provided by the present invention has better and unexpected technical effects for anti-cellular aging, and has significant practical significance for the preparation of Chinese herbal products for anti-cellular aging. DETAILED DESCRIPTION

[0034] The following will clearly and completely describe the technical solutions of the present invention in conjunction with the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0035] The present invention provides an anti-cell aging composition, comprising, by weight:

[0036]

[0037] In the present invention, the anti-cellular aging composition comprises 3 to 9 parts of Chinese herbal extract A, such as 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts or 9 parts, etc. The Chinese herbal extract A is extracted from Centella asiatica.

[0038] In some embodiments of the present invention, the Chinese herbal extract A is obtained by subjecting Centella asiatica to carbon dioxide extraction in the presence of an entrainer, wherein the entrainer is 40-50 wt% ethanol, preferably 45 wt% ethanol.

[0039] For example, in some specific embodiments of the present invention, the Chinese medicine extract A is prepared according to the following method:

[0040] Centella asiatica is taken, crushed to 60-80 mesh, and loaded into an extraction kettle. The temperature is set to 50-80°C, preferably 60°C, the pressure is set to 15-30 MPa, preferably 20 MPa, the flow rate of the 45wt% ethanol entrainer is (0.3-0.6 mL / min, preferably 0.4 mL / min), and the CO2 flow rate is (1-5 mL / min, preferably 2 mL / min). After the parameters are stabilized, the effluent is collected and extracted for 1-3 hours, preferably 2 hours. The filtrate is concentrated and dried under reduced pressure to obtain a traditional Chinese medicine extract A with anti-cell aging properties.

[0041] In the present invention, the anti-cellular aging composition comprises 2 to 8 parts of Chinese herbal extract B, such as 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts or 8 parts, etc. The Chinese herbal extract B is extracted from ginseng.

[0042] In some embodiments of the present invention, the Chinese herbal extract B is obtained by ultrasonic extraction of a mixture of ginseng and a solvent, wherein the solvent is 60-80 wt% ethanol, preferably 70 wt% ethanol. The solid-liquid ratio of ginseng to solvent is 1:20-1:35 g / mL, such as 1:20 g / mL, 1:22 g / mL, 1:25 g / mL, 1:27 g / mL, 1:30 g / mL, 1:32 g / mL, or 1:35 g / mL. The extraction is performed 1 to 3 times, preferably 2 times, and the extraction further includes concentration and drying steps.

[0043] For example, in some specific embodiments of the present invention, the Chinese herbal extract B is prepared according to the following method:

[0044] Ginseng is crushed and passed through a 30-40 mesh sieve, 60-80 wt% ethanol is added at a solid-liquid ratio of 1:20-1:35 g / mL, and the mixture is mixed. Ultrasonic power is 250-400 W, preferably 300 W, ultrasonic time is 20-50 min, preferably 30 min, and ultrasonic temperature is 30-50° C., preferably 40° C. Extraction is performed 1-3 times, preferably 2 times. The extracts from multiple extractions are combined, filtered, concentrated under reduced pressure, the solvent is removed, and the concentrate is dried, preferably freeze-dried, to obtain a traditional Chinese medicine extract B with anti-cell aging properties.

[0045] The present invention has no particular limitation on the source of the ginseng, and any commercially available product may be used.

[0046] In the present invention, the anti-cellular aging composition comprises 4 to 8 parts of Chinese herbal extract C, such as 4 parts, 5 parts, 6 parts, 7 parts, or 8 parts. The raw materials for extracting the Chinese herbal extract C are screened and preferably include Zanthoxylum bungeanum and ginger root to ensure that the two are extracted using the same method. Specifically, the composition preferably comprises 1 part of Zanthoxylum bungeanum and 1 to 3 parts of ginger root, i.e., 1 part of Zanthoxylum bungeanum and 1 part of ginger root; or 1 part of Zanthoxylum bungeanum and 1.5 parts of ginger root; or 1 part of Zanthoxylum bungeanum and 2 parts of ginger root; or 1 part of Zanthoxylum bungeanum and 2.5 parts of ginger root; or 1 part of Zanthoxylum bungeanum and 3 parts of ginger root.

[0047] In some embodiments of the present invention, the Chinese herbal extract C is obtained by steam distilling a mixture of Zanthoxylum bungeanum, ginger root, and water to extract volatile oil, followed by drying the volatile oil. The ratio of the total mass of the Zanthoxylum bungeanum and ginger root to the mass of water is 1:(10-15), such as 1:10, 1:11, 1:12, 1:13, 1:14, or 1:15.

[0048] For example, in some specific embodiments of the present invention, the Chinese medicine extract C is prepared according to the following method:

[0049] Take 8 parts of Zanthoxylum bungeanum and 12 parts of ginger root, grind them into fine powder, add 10 to 15 times the amount of water, preferably 12 times the amount of water, soak for 1 hour, extract volatile oil by steam distillation for 5 hours, collect the volatile oil, and freeze-dry to obtain a traditional Chinese medicine extract C with anti-cell aging effect.

[0050] In the present invention, the anti-cellular aging composition comprises 5 to 11 parts of the Chinese herbal extract D, such as 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts or 11 parts. The raw materials for extracting the Chinese herbal extract C are screened and preferably comprise Gentiana macrophylla and Gentiana scabra, so as to ensure that the two are extracted using the same method. Specifically, it preferably comprises 1 part of Gentiana macrophylla and 0.8 to 2 parts of Gentiana scabra, i.e., 1 part of Gentiana macrophylla and 0.8 part of Gentiana scabra; or 1 part of Gentiana macrophylla and 1 part of Gentiana scabra, or 1 part of Gentiana macrophylla and 1.2 parts of Gentiana scabra, or 1 part of Gentiana macrophylla and 1.5 parts of Gentiana scabra, or 1 part of Gentiana macrophylla and 2 parts of Gentiana scabra, etc.

[0051] In some embodiments of the present invention, the Chinese herbal extract D is obtained by flash extraction of a mixture of Gentiana macrophylla, Gentiana scabra and a solvent; the extraction conditions are set as follows: extraction temperature 55-70°C, preferably 62°C; extraction voltage 110-120V, preferably 112V; extraction time 60-120s, preferably 90s; the solvent is 40-60wt% ethanol, preferably 50wt% ethanol. The ratio of the total mass of Gentiana macrophylla and Gentiana scabra to the volume of the solvent is 1g:(50-70)mL, preferably 1g:60mL. The number of extractions is 1-3 times, preferably 3 times, and the extraction further includes the steps of concentration and drying.

[0052] For example, in some specific embodiments of the present invention, the Chinese medicine extract D is prepared according to the following method:

[0053] Take 6 parts of Gentiana macrophylla and 8 parts of Gentiana scabra, crush them through 60 mesh, add 50wt% ethanol solvent, and mix them evenly at a solid-liquid ratio of 1g:60mL. Set the extraction temperature to 62°C, the extraction voltage to 112V, and the extraction time to 90s. Extract three times, combine the extracts, concentrate under reduced pressure at 60°C, and spray dry to obtain a traditional Chinese medicine extract D with anti-cell aging properties.

[0054] In order to screen and optimize the formula of the above-mentioned anti-cellular aging composition, the present invention further uses the number of Chinese herbal extracts A, B, C, and D as factors, determines three levels for each factor, and designs an orthogonal test table, as shown in Table 1 below.

[0055] Table 1

[0056]

[0057] Furthermore, according to Table 1, the present invention designs the formulas of nine groups of anti-cellular aging compositions, namely, I, II, III, IV, V, VI, VII, VIII, and IX, as shown in Table 2.

[0058] Table 2

[0059] Serial number Chinese herbal medicine extract A Chinese herbal medicine extract B Chinese herbal medicine extract C Chinese herbal medicine extract D 1 3 2 4 5 2 3 5 6 8 3 3 8 8 11 4 6 2 6 11 5 6 5 8 5 6 6 8 4 8 7 9 2 8 8 8 9 5 4 11 9 9 8 6 5

[0060] The present invention mixed the Chinese herbal extracts A to D according to the above ratio to prepare nine groups of anti-cellular aging compositions (corresponding to the compositions obtained in Examples 1 to 9) including I, II, III, IV, V, VI, VII, VIII, and IX. Pharmacodynamic studies were conducted to comprehensively evaluate their anti-cellular aging effects in order to screen and optimize the formulations of the compositions.

[0061] In view of the anti-aging effect of the composition of the above-mentioned Chinese herbal extracts A to D, the present invention further provides an anti-cellular aging product comprising the above-mentioned composition, wherein the product includes an essence, lotion or cream.

[0062] In summary, the present invention separates, extracts and purifies the effective ingredients of different Chinese medicinal materials, and screens and proportions the extract components, and finally determines that the above-mentioned Chinese medicinal extract A, Chinese medicinal extract B, Chinese medicinal extract C, and Chinese medicinal extract D are combined to form a new anti-aging composition formula. Through cell experimental research, it is verified that the composition provided by the present invention can significantly exert the effect of anti-cellular aging, laying the foundation for providing a product with excellent anti-aging performance.

[0063] In order to further illustrate the present invention, the following examples are provided for detailed description. The experimental raw materials used in the following examples of the present invention are all commonly available commercial products.

[0064] Example 1

[0065] A Chinese herbal extract A with anti-cell aging properties:

[0066] Take 8 parts of Centella asiatica, crush it through 60 mesh, put it into an extraction kettle, set the temperature to 60°C, the pressure to 20 MPa, the flow rate of the 45wt% ethanol entrainer (0.4mL / min), and the CO2 flow rate (2mL / min), collect the outflow material after the parameters are stable, and extract and collect for 2 hours. The filtrate is concentrated and dried under reduced pressure to obtain a traditional Chinese medicine extract A with anti-cellular aging properties.

[0067] A Chinese herbal extract B with anti-cell aging properties:

[0068] Take 10 parts of ginseng, crush them through a 30-mesh sieve, add 70wt% ethanol, with a solid-liquid ratio of 1:25g / mL, mix well, ultrasonically control the power of 300w, the ultrasonic time of 30min, and the ultrasonic temperature of 40°C, extract twice, combine the two extracts, filter, concentrate under reduced pressure, remove the solvent, and freeze-dry the concentrate to obtain a traditional Chinese medicine extract B with anti-cellular aging properties.

[0069] A Chinese herbal extract C with anti-cell aging properties:

[0070] Take 8 parts of Zanthoxylum bungeanum and 12 parts of ginger root, grind them into fine powder, add 12 times the amount of water and soak for 1 hour, use steam distillation to extract volatile oil for 5 hours, collect the volatile oil, and freeze-dry to obtain a traditional Chinese medicine extract C with anti-cellular aging effect.

[0071] A Chinese herbal extract D with anti-cell aging properties:

[0072] Take 6 parts of Gentiana macrophylla and 8 parts of Gentiana scabra, crush them through 60 mesh, add 50wt% ethanol solvent, and mix them evenly at a solid-liquid ratio of 1:60 (g:mL). Set the extraction temperature to 62°C, the extraction voltage to 112V, and the extraction time to 90s. Extract three times, combine the extracts, concentrate under reduced pressure at 60°C, and spray dry to obtain a traditional Chinese medicine extract D with anti-cellular aging properties.

[0073] Take 3 parts of the Chinese herbal extract A with anti-cellular aging, 2 parts of the Chinese herbal extract B with anti-cellular aging, 4 parts of the Chinese herbal extract C with anti-cellular aging, and 5 parts of the Chinese herbal extract D with anti-cellular aging, mix them evenly, add an appropriate amount of sucrose, and make 1000 g as Example 1 group.

[0074] Example 2

[0075] A Chinese herbal extract A with anti-cell aging properties:

[0076] Take 8 parts of Centella asiatica, crush it through 60 mesh, put it into an extraction kettle, set the temperature to 60°C, the pressure to 20 MPa, the flow rate of the 45wt% ethanol entrainer (0.4mL / min), and the CO2 flow rate (2mL / min), collect the outflow material after the parameters are stable, and extract and collect for 2 hours. The filtrate is concentrated and dried under reduced pressure to obtain a traditional Chinese medicine extract A with anti-cellular aging properties.

[0077] A Chinese herbal extract B with anti-cell aging properties:

[0078] Take 10 parts of ginseng, crush them through a 30-mesh sieve, add 70wt% ethanol, with a solid-liquid ratio of 1:20g / mL, mix well, ultrasonically control the power of 300w, the ultrasonic time of 30min, and the ultrasonic temperature of 40°C, extract twice, combine the two extracts, filter, concentrate under reduced pressure, remove the solvent, and freeze-dry the concentrate to obtain a traditional Chinese medicine extract B with anti-cellular aging properties.

[0079] A Chinese herbal extract C with anti-cell aging properties:

[0080] Take 8 parts of Zanthoxylum bungeanum and 12 parts of ginger root, grind them into fine powder, add 12 times the amount of water and soak for 1 hour, use steam distillation to extract volatile oil for 5 hours, collect the volatile oil, and freeze-dry to obtain a traditional Chinese medicine extract C with anti-cellular aging effect.

[0081] A Chinese herbal extract D with anti-cell aging properties:

[0082] Take 6 parts of Gentiana macrophylla and 8 parts of Gentiana scabra, crush them through 60 mesh, add 50wt% ethanol solvent, and mix them evenly at a solid-liquid ratio of 1:60 (g:mL). Set the extraction temperature to 62°C, the extraction voltage to 112V, and the extraction time to 90s. Extract three times, combine the extracts, concentrate under reduced pressure at 60°C, and spray dry to obtain a traditional Chinese medicine extract D with anti-cellular aging properties.

[0083] Take 3 parts of a traditional Chinese medicine extract A with anti-cellular aging, 5 parts of a traditional Chinese medicine extract B with anti-cellular aging, 6 parts of a traditional Chinese medicine extract C with anti-cellular aging, and 8 parts of a traditional Chinese medicine extract D with anti-cellular aging, mix them evenly, add an appropriate amount of sucrose, and make 1000 g, as Example 2 group.

[0084] Example 3

[0085] A Chinese herbal extract A with anti-cell aging properties:

[0086] Take 8 parts of Centella asiatica, crush it through 60 mesh, put it into an extraction kettle, set the temperature to 60°C, the pressure to 20 MPa, the flow rate of the 45wt% ethanol entrainer (0.4mL / min), and the CO2 flow rate (2mL / min), collect the outflow material after the parameters are stable, and extract and collect for 2 hours. The filtrate is concentrated and dried under reduced pressure to obtain a traditional Chinese medicine extract A with anti-cellular aging properties.

[0087] A Chinese herbal extract B with anti-cell aging properties:

[0088] Take 10 parts of ginseng, crush them through a 30-mesh sieve, add 70wt% ethanol, and mix them at a solid-liquid ratio of 1:35 (g / mL). Ultrasonic power is 300w, ultrasonic time is 30min, and ultrasonic temperature is 40°C. Extract twice, combine the two extracts, filter, concentrate under reduced pressure, remove the solvent, and freeze-dry the concentrate to obtain a traditional Chinese medicine extract B with anti-cellular aging properties.

[0089] A Chinese herbal extract C with anti-cell aging properties:

[0090] Take 8 parts of Zanthoxylum bungeanum and 12 parts of ginger root, grind them into fine powder, add 12 times the amount of water and soak for 1 hour, use steam distillation to extract volatile oil for 5 hours, collect the volatile oil, and freeze-dry to obtain a traditional Chinese medicine extract C with anti-cellular aging effect.

[0091] A Chinese herbal extract D with anti-cell aging properties:

[0092] Take 6 parts of Gentiana macrophylla and 8 parts of Gentiana scabra, crush them through 60 mesh, add 50wt% ethanol solvent, and mix them evenly at a solid-liquid ratio of 1:60 (g:mL). Set the extraction temperature to 62°C, the extraction voltage to 112V, and the extraction time to 90s. Extract three times, combine the extracts, concentrate under reduced pressure at 60°C, and spray dry to obtain a traditional Chinese medicine extract D with anti-cellular aging properties.

[0093] Take 3 parts of a traditional Chinese medicine extract A with anti-cellular aging, 8 parts of a traditional Chinese medicine extract B with anti-cellular aging, 8 parts of a traditional Chinese medicine extract C with anti-cellular aging, and 11 parts of a traditional Chinese medicine extract D with anti-cellular aging, mix them evenly, add an appropriate amount of sucrose, and make 1000 g, as Example 3 group.

[0094] Example 4

[0095] A Chinese herbal extract A with anti-cell aging properties:

[0096] Take 8 parts of Centella asiatica, crush it through 60 mesh, put it into an extraction kettle, set the temperature to 60°C, the pressure to 20 MPa, the flow rate of the 45wt% ethanol entrainer (0.4mL / min), and the CO2 flow rate (2mL / min), collect the outflow material after the parameters are stable, and extract and collect for 2 hours. The filtrate is concentrated and dried under reduced pressure to obtain a traditional Chinese medicine extract A with anti-cellular aging properties.

[0097] A Chinese herbal extract B with anti-cell aging properties:

[0098] Take 10 parts of ginseng, crush them through a 30-mesh sieve, add 70wt% ethanol, with a solid-liquid ratio of 1:35g / mL, mix well, ultrasonically control the power of 300w, the ultrasonic time of 30min, and the ultrasonic temperature of 40°C, extract twice, combine the two extracts, filter, concentrate under reduced pressure, remove the solvent, and freeze-dry the concentrate to obtain a traditional Chinese medicine extract B with anti-cellular aging properties.

[0099] A Chinese herbal extract C with anti-cell aging properties:

[0100] Take 8 parts of Zanthoxylum bungeanum and 12 parts of ginger root, grind them into fine powder, add 12 times the amount of water and soak for 1 hour, use steam distillation to extract volatile oil for 5 hours, collect the volatile oil, and freeze-dry to obtain a traditional Chinese medicine extract C with anti-cellular aging effect.

[0101] A Chinese herbal extract D with anti-cell aging properties:

[0102] Take 6 parts of Gentiana macrophylla and 8 parts of Gentiana scabra, crush them through 60 mesh, add 50wt% ethanol solvent, and mix them evenly at a solid-liquid ratio of 1:60 (g:mL). Set the extraction temperature to 62°C, the extraction voltage to 112V, and the extraction time to 90s. Extract three times, combine the extracts, concentrate under reduced pressure at 60°C, and spray dry to obtain a traditional Chinese medicine extract D with anti-cellular aging properties.

[0103] Take 6 parts of a Chinese herbal extract A with anti-cellular aging, 2 parts of a Chinese herbal extract B with anti-cellular aging, 6 parts of a Chinese herbal extract C with anti-cellular aging, and 11 parts of a Chinese herbal extract D with anti-cellular aging, mix them evenly, add an appropriate amount of sucrose, and make 1000 g, as Example 4 group.

[0104] Example 5

[0105] A Chinese herbal extract A with anti-cell aging properties:

[0106] Take 8 parts of Centella asiatica, crush it through 60 mesh, put it into an extraction kettle, set the temperature to 60°C, the pressure to 20 MPa, the flow rate of the 45wt% ethanol entrainer (0.4mL / min), and the CO2 flow rate (2mL / min), collect the outflow material after the parameters are stable, and extract and collect for 2 hours. The filtrate is concentrated and dried under reduced pressure to obtain a traditional Chinese medicine extract A with anti-cellular aging properties.

[0107] A Chinese herbal extract B with anti-cell aging properties:

[0108] Take 10 parts of ginseng, crush them through a 30-mesh sieve, add 70wt% ethanol, with a solid-liquid ratio of 1:25g / mL, mix well, ultrasonically control the power of 300w, the ultrasonic time of 30min, and the ultrasonic temperature of 40°C, extract twice, combine the two extracts, filter, concentrate under reduced pressure, remove the solvent, and freeze-dry the concentrate to obtain a traditional Chinese medicine extract B with anti-cellular aging properties.

[0109] A Chinese herbal extract C with anti-cell aging properties:

[0110] Take 8 parts of Zanthoxylum bungeanum and 12 parts of ginger root, grind them into fine powder, add 12 times the amount of water and soak for 1 hour, use steam distillation to extract volatile oil for 5 hours, collect the volatile oil, and freeze-dry to obtain a traditional Chinese medicine extract C with anti-cellular aging effect.

[0111] A Chinese herbal extract D with anti-cell aging properties:

[0112] Take 6 parts of Gentiana macrophylla and 8 parts of Gentiana scabra, crush them through 60 mesh, add 50wt% ethanol solvent, and mix them evenly at a solid-liquid ratio of 1:60 (g:mL). Set the extraction temperature to 62°C, the extraction voltage to 112V, and the extraction time to 90s. Extract three times, combine the extracts, concentrate under reduced pressure at 60°C, and spray dry to obtain a traditional Chinese medicine extract D with anti-cellular aging properties.

[0113] Take 6 parts of a traditional Chinese medicine extract A with anti-cellular aging, 5 parts of a traditional Chinese medicine extract B with anti-cellular aging, 8 parts of a traditional Chinese medicine extract C with anti-cellular aging, and 5 parts of a traditional Chinese medicine extract D with anti-cellular aging, mix them evenly, add an appropriate amount of sucrose, and make 1000 g, as Example 5 group.

[0114] Example 6

[0115] A Chinese herbal extract A with anti-cell aging properties:

[0116] Take 8 parts of Centella asiatica, crush it through 60 mesh, put it into an extraction kettle, set the temperature to 60°C, the pressure to 20 MPa, the flow rate of the 45wt% ethanol entrainer (0.4mL / min), and the CO2 flow rate (2mL / min), collect the outflow material after the parameters are stable, and extract and collect for 2 hours. The filtrate is concentrated and dried under reduced pressure to obtain a traditional Chinese medicine extract A with anti-cellular aging properties.

[0117] A Chinese herbal extract B with anti-cell aging properties:

[0118] Take 10 parts of ginseng, crush them through a 30-mesh sieve, add 70wt% ethanol, with a solid-liquid ratio of 1:25g / mL, mix well, ultrasonically control the power of 300w, the ultrasonic time of 30min, and the ultrasonic temperature of 40°C, extract twice, combine the two extracts, filter, concentrate under reduced pressure, remove the solvent, and freeze-dry the concentrate to obtain a traditional Chinese medicine extract B with anti-cellular aging properties.

[0119] A Chinese herbal extract C with anti-cell aging properties:

[0120] Take 8 parts of Zanthoxylum bungeanum and 12 parts of ginger root, grind them into fine powder, add 12 times the amount of water and soak for 1 hour, use steam distillation to extract volatile oil for 5 hours, collect the volatile oil, and freeze-dry to obtain a traditional Chinese medicine extract C with anti-cellular aging effect.

[0121] A Chinese herbal extract D with anti-cell aging properties:

[0122] Take 6 parts of Gentiana macrophylla and 8 parts of Gentiana scabra, crush them through 60 mesh, add 50wt% ethanol solvent, and mix them evenly at a solid-liquid ratio of 1:60 (g:mL). Set the extraction temperature to 62°C, the extraction voltage to 112V, and the extraction time to 90s. Extract three times, combine the extracts, concentrate under reduced pressure at 60°C, and spray dry to obtain a traditional Chinese medicine extract D with anti-cellular aging properties.

[0123] Take 6 parts of a traditional Chinese medicine extract A with anti-cellular aging, 8 parts of a traditional Chinese medicine extract B with anti-cellular aging, 4 parts of a traditional Chinese medicine extract C with anti-cellular aging, and 8 parts of a traditional Chinese medicine extract D with anti-cellular aging, mix them evenly, add an appropriate amount of sucrose, and make 1000 g, as Example 6 group.

[0124] Example 7

[0125] A Chinese herbal extract A with anti-cell aging properties:

[0126] Take 8 parts of Centella asiatica, crush it through 60 mesh, put it into an extraction kettle, set the temperature to 60°C, the pressure to 20 MPa, the flow rate of the 45wt% ethanol entrainer (0.4mL / min), and the CO2 flow rate (2mL / min), collect the outflow material after the parameters are stable, and extract and collect for 2 hours. The filtrate is concentrated and dried under reduced pressure to obtain a traditional Chinese medicine extract A with anti-cellular aging properties.

[0127] A Chinese herbal extract B with anti-cell aging properties:

[0128] Take 10 parts of ginseng, crush them through a 30-mesh sieve, add 70wt% ethanol, with a solid-liquid ratio of 1:25g / mL, mix well, ultrasonically control the power of 300w, the ultrasonic time of 30min, and the ultrasonic temperature of 40°C, extract twice, combine the two extracts, filter, concentrate under reduced pressure, remove the solvent, and freeze-dry the concentrate to obtain a traditional Chinese medicine extract B with anti-cellular aging properties.

[0129] A Chinese herbal extract C with anti-cell aging properties:

[0130] Take 8 parts of Zanthoxylum bungeanum and 12 parts of ginger root, grind them into fine powder, add 12 times the amount of water and soak for 1 hour, use steam distillation to extract volatile oil for 5 hours, collect the volatile oil, and freeze-dry to obtain a traditional Chinese medicine extract C with anti-cellular aging effect.

[0131] A Chinese herbal extract D with anti-cell aging properties:

[0132] Take 6 parts of Gentiana macrophylla and 8 parts of Gentiana scabra, crush them through 60 mesh, add 50wt% ethanol solvent, and mix them evenly at a solid-liquid ratio of 1:60 (g:mL). Set the extraction temperature to 62°C, the extraction voltage to 112V, and the extraction time to 90s. Extract three times, combine the extracts, concentrate under reduced pressure at 60°C, and spray dry to obtain a traditional Chinese medicine extract D with anti-cellular aging properties.

[0133] Take 9 parts of a Chinese herbal extract A with anti-cellular aging, 2 parts of a Chinese herbal extract B with anti-cellular aging, 8 parts of a Chinese herbal extract C with anti-cellular aging, and 8 parts of a Chinese herbal extract D with anti-cellular aging, mix them evenly, add an appropriate amount of sucrose, and make 1000 g, as Example 7 group.

[0134] Example 8

[0135] A Chinese herbal extract A with anti-cell aging properties:

[0136] Take 8 parts of Centella asiatica, crush it through 60 mesh, put it into an extraction kettle, set the temperature to 60°C, the pressure to 20 MPa, the flow rate of the 45wt% ethanol entrainer (0.4mL / min), and the CO2 flow rate (2mL / min), collect the outflow material after the parameters are stable, and extract and collect for 2 hours. The filtrate is concentrated and dried under reduced pressure to obtain a traditional Chinese medicine extract A with anti-cellular aging properties.

[0137] A Chinese herbal extract B with anti-cell aging properties:

[0138] Take 10 parts of ginseng, crush them through a 30-mesh sieve, add 70wt% ethanol, with a solid-liquid ratio of 1:25g / mL, mix well, ultrasonically control the power of 300w, the ultrasonic time of 30min, and the ultrasonic temperature of 40°C, extract twice, combine the two extracts, filter, concentrate under reduced pressure, remove the solvent, and freeze-dry the concentrate to obtain a traditional Chinese medicine extract B with anti-cellular aging properties.

[0139] A Chinese herbal extract C with anti-cell aging properties:

[0140] Take 8 parts of Zanthoxylum bungeanum and 12 parts of ginger root, grind them into fine powder, add 12 times the amount of water and soak for 1 hour, use steam distillation to extract volatile oil for 5 hours, collect the volatile oil, and freeze-dry to obtain a traditional Chinese medicine extract C with anti-cellular aging effect.

[0141] A Chinese herbal extract D with anti-cell aging properties:

[0142] Take 6 parts of Gentiana macrophylla and 8 parts of Gentiana scabra, crush them through 60 mesh, add 50wt% ethanol solvent, and mix them evenly at a solid-liquid ratio of 1:60 (g:mL). Set the extraction temperature to 62°C, the extraction voltage to 112V, and the extraction time to 90s. Extract three times, combine the extracts, concentrate under reduced pressure at 60°C, and spray dry to obtain a traditional Chinese medicine extract D with anti-cellular aging properties.

[0143] Take 9 parts of a traditional Chinese medicine extract A with anti-cellular aging, 5 parts of a traditional Chinese medicine extract B with anti-cellular aging, 4 parts of a traditional Chinese medicine extract C with anti-cellular aging, and 11 parts of a traditional Chinese medicine extract D with anti-cellular aging, mix them evenly, add an appropriate amount of sucrose, and make 1000 g, as Example 8 group.

[0144] Example 9

[0145] A Chinese herbal extract A with anti-cell aging properties:

[0146] Take 8 parts of Centella asiatica, crush it through 60 mesh, put it into an extraction kettle, set the temperature to 60°C, the pressure to 20 MPa, the flow rate of the 45wt% ethanol entrainer (0.4mL / min), and the CO2 flow rate (2mL / min), collect the outflow material after the parameters are stable, and extract and collect for 2 hours. The filtrate is concentrated and dried under reduced pressure to obtain a traditional Chinese medicine extract A with anti-cellular aging properties.

[0147] A Chinese herbal extract B with anti-cell aging properties:

[0148] Take 10 parts of ginseng, crush them through a 30-mesh sieve, add 70wt% ethanol, with a solid-liquid ratio of 1:25g / mL, mix well, ultrasonically control the power of 300w, the ultrasonic time of 30min, and the ultrasonic temperature of 40°C, extract twice, combine the two extracts, filter, concentrate under reduced pressure, remove the solvent, and freeze-dry the concentrate to obtain a traditional Chinese medicine extract B with anti-cellular aging properties.

[0149] A Chinese herbal extract C with anti-cell aging properties:

[0150] Take 8 parts of Zanthoxylum bungeanum and 12 parts of ginger root, grind them into fine powder, add 12 times the amount of water and soak for 1 hour, use steam distillation to extract volatile oil for 5 hours, collect the volatile oil, and freeze-dry to obtain a traditional Chinese medicine extract C with anti-cellular aging effect.

[0151] A Chinese herbal extract D with anti-cell aging properties:

[0152] Take 6 parts of Gentiana macrophylla and 8 parts of Gentiana scabra, crush them through 60 mesh, add 50wt% ethanol solvent, and mix them evenly at a solid-liquid ratio of 1:60 (g:mL). Set the extraction temperature to 62°C, the extraction voltage to 112V, and the extraction time to 90s. Extract three times, combine the extracts, concentrate under reduced pressure at 60°C, and spray dry to obtain a traditional Chinese medicine extract D with anti-cellular aging properties.

[0153] Take 9 parts of a Chinese herbal extract A with anti-cellular aging, 8 parts of a Chinese herbal extract B with anti-cellular aging, 6 parts of a Chinese herbal extract C with anti-cellular aging, and 5 parts of a Chinese herbal extract D with anti-cellular aging, mix them evenly, add an appropriate amount of sucrose, and make 1000 g, as Example 9 group.

[0154] Comparative Example 1

[0155] A Chinese herbal extract A with anti-cell aging properties:

[0156] Take 8 parts of Centella asiatica, crush it through 60 mesh, put it into an extraction kettle, set the temperature to 60°C, the pressure to 20 MPa, the flow rate of the 45wt% ethanol entrainer (0.4mL / min), and the CO2 flow rate (2mL / min), collect the outflow material after the parameters are stable, and extract and collect for 2 hours. The filtrate is concentrated and dried under reduced pressure to obtain a traditional Chinese medicine extract A with anti-cellular aging properties.

[0157] A Chinese herbal extract B with anti-cell aging properties:

[0158] Take 10 parts of ginseng, crush them through a 30-mesh sieve, add 70wt% ethanol, with a solid-liquid ratio of 1:25g / mL, mix well, ultrasonically control the power of 300w, the ultrasonic time of 30min, and the ultrasonic temperature of 40°C, extract twice, combine the two extracts, filter, concentrate under reduced pressure, remove the solvent, and freeze-dry the concentrate to obtain a traditional Chinese medicine extract B with anti-cellular aging properties.

[0159] 3 parts of a Chinese herbal extract A with anti-cellular aging properties and 8 parts of a Chinese herbal extract B with anti-cellular aging properties were mixed evenly, and an appropriate amount of sucrose was added to prepare 1000 g, which was used as comparative example 1.

[0160] Comparative Example 2

[0161] A Chinese herbal extract A with anti-cell aging properties:

[0162] Take 8 parts of Centella asiatica, crush it through 60 mesh, put it into an extraction kettle, set the temperature to 60°C, the pressure to 20 MPa, the flow rate of the 45wt% ethanol entrainer (0.4mL / min), and the CO2 flow rate (2mL / min), collect the outflow material after the parameters are stable, and extract and collect for 2 hours. The filtrate is concentrated and dried under reduced pressure to obtain a traditional Chinese medicine extract A with anti-cellular aging properties.

[0163] A Chinese medicine extract D with anti-cell aging properties:

[0164] Take 6 parts of Gentiana macrophylla and 8 parts of Gentiana scabra, crush them through 60 mesh, add 50wt% ethanol solvent, and mix them evenly at a solid-liquid ratio of 1:60 (g:mL). Set the extraction temperature to 62°C, the extraction voltage to 112V, and the extraction time to 90s. Extract three times, combine the extracts, concentrate under reduced pressure at 60°C, and spray dry to obtain a traditional Chinese medicine extract D with anti-cellular aging properties.

[0165] Take 3 parts of a Chinese herbal extract A with anti-cellular aging and 11 parts of a Chinese herbal extract D with anti-cellular aging, mix them evenly, add an appropriate amount of sucrose to make 1000 g, and use them as the second comparative example.

[0166] Comparative Example 3

[0167] A Chinese herbal extract A with anti-cell aging properties:

[0168] Take 8 parts of Centella asiatica, crush it through 60 mesh, put it into an extraction kettle, set the temperature to 60°C, the pressure to 20 MPa, the flow rate of the 45wt% ethanol entrainer (0.4mL / min), and the CO2 flow rate (2mL / min), collect the outflow material after the parameters are stable, and extract and collect for 2 hours. The filtrate is concentrated and dried under reduced pressure to obtain a traditional Chinese medicine extract A with anti-cellular aging properties.

[0169] A Chinese herbal extract C with anti-cell aging properties:

[0170] Take 8 parts of Zanthoxylum bungeanum and 12 parts of ginger root, grind them into fine powder, add 12 times the amount of water and soak for 1 hour, use steam distillation to extract volatile oil for 5 hours, collect the volatile oil, and freeze-dry to obtain a traditional Chinese medicine extract C with anti-cellular aging effect.

[0171] Take 3 parts of a Chinese herbal extract A with anti-cellular aging effect and 8 parts of a Chinese herbal extract C with anti-cellular aging effect, mix them evenly, add appropriate amount of sucrose to make 1000 g, and use them as group 3 of comparative example.

[0172] Comparative Example 4

[0173] A Chinese herbal extract A with anti-cell aging properties:

[0174] Take 8 parts of Centella asiatica, crush it through 60 mesh, put it into an extraction kettle, set the temperature to 60°C, the pressure to 20 MPa, the flow rate of the 45wt% ethanol entrainer (0.4mL / min), and the CO2 flow rate (2mL / min), collect the outflow material after the parameters are stable, and extract and collect for 2 hours. The filtrate is concentrated and dried under reduced pressure to obtain a traditional Chinese medicine extract A with anti-cellular aging properties.

[0175] A Chinese herbal extract B with anti-cell aging properties:

[0176] Take 10 parts of ginseng, crush them through a 30-mesh sieve, add 70wt% ethanol, with a solid-liquid ratio of 1:25g / mL, mix well, ultrasonically control the power of 300w, the ultrasonic time of 30min, and the ultrasonic temperature of 40°C, extract twice, combine the two extracts, filter, concentrate under reduced pressure, remove the solvent, and freeze-dry the concentrate to obtain a traditional Chinese medicine extract B with anti-cellular aging properties.

[0177] A Chinese herbal extract C with anti-cell aging properties:

[0178] Take 8 parts of Zanthoxylum bungeanum and 12 parts of ginger root, grind them into fine powder, add 12 times the amount of water and soak for 1 hour, use steam distillation to extract volatile oil for 5 hours, collect the volatile oil, and freeze-dry to obtain a traditional Chinese medicine extract C with anti-cellular aging effect.

[0179] Take 3 parts of a Chinese herbal extract A with anti-cellular aging, 8 parts of a Chinese herbal extract B with anti-cellular aging, and 8 parts of a Chinese herbal extract C with anti-cellular aging, mix them evenly, add an appropriate amount of sucrose, and make 1000 g, as group 4 of comparative example.

[0180] Comparative Example 5

[0181] A Chinese herbal extract A with anti-cell aging properties:

[0182] Take 8 parts of Centella asiatica, crush it through 60 mesh, put it into an extraction kettle, set the temperature to 60°C, the pressure to 20 MPa, the flow rate of the 45wt% ethanol entrainer (0.4mL / min), and the CO2 flow rate (2mL / min), collect the outflow material after the parameters are stable, and extract and collect for 2 hours. The filtrate is concentrated and dried under reduced pressure to obtain a traditional Chinese medicine extract A with anti-cellular aging properties.

[0183] A Chinese herbal extract B with anti-cell aging properties:

[0184] Take 10 parts of ginseng, crush them through a 30-mesh sieve, add 70wt% ethanol, with a solid-liquid ratio of 1:25g / mL, mix well, ultrasonically control the power of 300w, the ultrasonic time of 30min, and the ultrasonic temperature of 40°C, extract twice, combine the two extracts, filter, concentrate under reduced pressure, remove the solvent, and freeze-dry the concentrate to obtain a traditional Chinese medicine extract B with anti-cellular aging properties.

[0185] A Chinese herbal extract D with anti-cell aging properties:

[0186] Take 6 parts of Gentiana macrophylla and 8 parts of Gentiana scabra, crush them through 60 mesh, add 50wt% ethanol solvent, and mix them evenly at a solid-liquid ratio of 1:60 (g:mL). Set the extraction temperature to 62°C, the extraction voltage to 112V, and the extraction time to 90s. Extract three times, combine the extracts, concentrate under reduced pressure at 60°C, and spray dry to obtain a traditional Chinese medicine extract D with anti-cellular aging properties.

[0187] Take 3 parts of a Chinese herbal extract A with anti-cellular aging, 8 parts of a Chinese herbal extract B with anti-cellular aging, and 11 parts of a Chinese herbal extract D with anti-cellular aging, mix them evenly, add an appropriate amount of sucrose, and make 1000 g, as group 5 of comparative examples.

[0188] Comparative Example 6

[0189] A Chinese herbal extract B with anti-cell aging properties:

[0190] Take 10 parts of ginseng, crush them through a 30-mesh sieve, add 70wt% ethanol, with a solid-liquid ratio of 1:25g / mL, mix well, ultrasonically control the power of 300w, the ultrasonic time of 30min, and the ultrasonic temperature of 40°C, extract twice, combine the two extracts, filter, concentrate under reduced pressure, remove the solvent, and freeze-dry the concentrate to obtain a traditional Chinese medicine extract B with anti-cellular aging properties.

[0191] A Chinese herbal extract C with anti-cell aging properties:

[0192] Take 8 parts of Zanthoxylum bungeanum and 12 parts of ginger root, grind them into fine powder, add 12 times the amount of water and soak for 1 hour, use steam distillation to extract volatile oil for 5 hours, collect the volatile oil, and freeze-dry to obtain a traditional Chinese medicine extract C with anti-cellular aging effect.

[0193] A Chinese herbal extract D with anti-cell aging properties:

[0194] Take 6 parts of Gentiana macrophylla and 8 parts of Gentiana scabra, crush them through 60 mesh, add 50wt% ethanol solvent, and mix them evenly at a solid-liquid ratio of 1:60 (g:mL). Set the extraction temperature to 62°C, the extraction voltage to 112V, and the extraction time to 90s. Extract three times, combine the extracts, concentrate under reduced pressure at 60°C, and spray dry to obtain a traditional Chinese medicine extract D with anti-cellular aging properties.

[0195] 8 parts of a Chinese herbal extract B with anti-cellular aging, 8 parts of a Chinese herbal extract C with anti-cellular aging, and 11 parts of a Chinese herbal extract D with anti-cellular aging were mixed evenly, and an appropriate amount of sucrose was added to make 1000 g, which was used as group 6 of comparative examples.

[0196] Comparative pharmacodynamics experimental study

[0197] 1. Establishment of UVB-damaged HaCaT cell model

[0198] HaCaT cells were seeded into a culture flask, 5 mL of MEM medium containing 10% fetal bovine serum was added, and the cells were cultured in a cell culture incubator at 37°C and 5% CO2. When the cells reached 70-80% confluency, they were passaged and digested with 0.25% trypsin for 7 minutes. The digestion was terminated by adding culture medium, and the cells were collected and centrifuged. The cell concentration was adjusted to 1×10 cells / mL using MEM medium containing 10% fetal bovine serum. 5 / mL and inoculated into culture plates.

[0199] HaCaT cells in the logarithmic growth phase were seeded into two 96-well plates. After the cells grew to 70-80% confluence, they were divided into a blank control group, a UVB radiation model group, a positive drug group (glycerol), and a drug administration experimental group (including Example 1-9 groups and Comparative Example 1-6 groups). Each group of cells was set up with 6 replicate wells.

[0200] 2. MTT assay for the effect on cell viability

[0201] HaCaT cells in the blank control group and UVB model group were cultured in MEM complete medium without drug for 6 h; the positive drug group (glycerol concentration was 50 mg / L, 100 μL) and the drug treatment experimental group (composition concentration was 50 mg / L, 100 μL) were cultured in MEM complete medium for 6 h. The culture medium was discarded, and an appropriate amount of PBS was added to cover the cells. The cells were then irradiated to establish the model at a dose of 60 mJ / cm 2 The blank control group was covered with aluminum foil. PBS was discarded, and culture medium containing 10% fetal bovine serum was added and the cells were placed in an incubator for further 24 h.

[0202] 3. Biochemical colorimetric detection of SOD, MDA and Hyp content

[0203] Each group of cells was treated according to the procedures in "I. Establishment of a UVB-damaged HaCaT Cell Model." Cell supernatants were collected 24 hours after irradiation and processed strictly according to the kit instructions from the Nanjing Jiancheng Bioengineering Institute. SOD, MDA, and Hyp levels were immediately measured. Six replicate wells were performed in each group, and the average value was calculated.

[0204] IV. Intracellular Type I Collagen Determination

[0205] HaCat cells in the logarithmic growth phase were seeded in 6-well plates at a density of 50,000 cells / mL and incubated in a 37°C, 5% CO2 incubator for 24 hours. The blank control group and UVB model group HaCaT cells were cultured with drug-free MEM complete culture medium for 6 hours; the positive drug group (glycerol concentration was 50 mg / L, 100 μL) and the drug-treated experimental group (composition concentration was 50 mg / L, 100 μL) were cultured with MEM complete culture medium for 6 hours and TNF-α (200 ng / mL) solution was added for 24 hours. The culture medium in the wells was removed, washed twice with PBS, 200 μL of lysis buffer containing PMSF was added to each well for 1 minute, and centrifuged at 10,000 r / min for 5 minutes. Subsequently, the intracellular COL-1 content was detected using a COL-1 ELISA kit and corrected with protein.

[0206] 5. Determination of intracellular MMP-1 content

[0207] HaCat cells in the logarithmic growth phase were seeded in 6-well plates at a density of 50,000 cells / mL and incubated in a 37°C, 5% CO2 incubator for 24 hours. The blank control group and UVB model group HaCaT cells were cultured with drug-free MEM complete culture medium for 6 hours; the positive drug group (glycerol concentration was 50 mg / L, 100 μL) and the drug-treated experimental group (composition concentration was 50 mg / L, 100 μL) were cultured with MEM complete culture medium for 6 hours and TNF-a solution was added for 24 hours. The culture medium in the wells was removed, washed twice with PBS, 200 μL of lysis buffer containing PMSF was added to each well for 1 minute, and centrifuged at 10,000 r / min for 5 minutes. Subsequently, the intracellular COL-1 content was detected using a COL-1 ELISA kit and corrected with protein.

[0208] Experimental results

[0209] 1. MTT assay to determine cell activity

[0210] Compared with the blank group, the cell survival rate of the model group was significantly decreased (P<0.05). Compared with the model group, the cell survival rate of the Example 1 to 9 groups was significantly increased (P<0.05). From the cell survival rate results, it can be seen that among the compositions of the Example 1 to 9 groups, the Example 3 group was superior to the positive drug group and the comparative example 1 to 6 groups, as shown in Table 3. It represents the sample mean ± plus or minus standard deviation (s), n = 10, indicating 10 tests.

[0211] Table 3 Cell activity of each group ( n=10)

[0212]

[0213]

[0214] Note: The shoulder marks represent the results of pairwise comparison by analysis of variance. Different letters in the same column represent significant differences (P < 0.05), while the same letters in the same column represent no significant differences (P > 0.05).

[0215] 2. Biochemical colorimetric assay of SOD, MDA and Hyp content

[0216] Compared with the blank group, the SOD and Hyp levels of the model group were significantly reduced (P<0.05), and the MDA level was significantly increased (P<0.05). Compared with the model group, the SOD and Hyp levels of the positive drug group and Example 1 to 9 groups were significantly increased (P<0.05), and the MDA level was significantly reduced (P<0.05). From the results of SOD, MDA and Hyp content, it can be seen that among the Example 1 to 9 groups, the Example 3 group was better than the positive drug group and the comparative example 1 to 6 groups, as shown in Table 4. It represents the sample mean ± plus or minus standard deviation (s), n = 10, indicating 10 tests.

[0217] Table 4 SOD, MDA and Hyp contents in each group ( n=10)

[0218]

[0219]

[0220] Note: The shoulder marks represent the results of pairwise comparison by analysis of variance. Different letters in the same column represent significant differences (P < 0.05), while the same letters in the same column represent no significant differences (P > 0.05).

[0221] 3. COL-1 Cell Results

[0222] Compared with the blank group, the intracellular COL-1 content of the model group was significantly reduced (P<0.05). Compared with the model group, the positive drug group and the Chinese medicine composition 1-9 groups were significantly increased (P<0.05). From the results of intracellular COL-1 content, it can be seen that among the Example 1-9 groups, the Example 3 group was superior to the positive drug group and the comparative example 1-6 groups, as shown in Table 5. It represents the sample mean ± plus or minus standard deviation (s), n = 10, indicating 10 tests.

[0223] Table 5 COL-1 content of cells in each group ( n=10)

[0224]

[0225]

[0226] Note: The shoulder marks represent the results of pairwise comparison by analysis of variance. Different letters in the same column represent significant differences (P < 0.05), while the same letters in the same column represent no significant differences (P > 0.05).

[0227] 4. Intracellular MMP-1 Results

[0228] Compared with the blank group, the intracellular MMP-1 content of the model group was significantly increased (P<0.05). Compared with the model group, the positive drug group and the Chinese medicine composition 1-9 groups were significantly reduced (P<0.05). From the results of the intracellular MMP-1 content, it can be seen that among the Example 1-9 groups, the Example 3 group was superior to the positive drug group and the comparative example group, as shown in Table 6. It represents the sample mean ± plus or minus standard deviation (s), n = 10, indicating 10 tests.

[0229] Table 6 MMP-1 content in each group of cells ( n=10)

[0230]

[0231]

[0232] Note: The shoulder marks represent the results of pairwise comparison by analysis of variance. Different letters in the same column represent significant differences (P < 0.05), while the same letters in the same column represent no significant differences (P > 0.05).

[0233] Combined with Tables 2 to 6 above, it can be seen that the Chinese medicine compositions of Examples 1 to 9 provided by the present invention have obvious cell proliferation promoting effects as shown by the MTT results. The SOD and MDA results show that they can improve antioxidant capacity, inhibit the expression of MMP-1, promote Hyp expression, and increase the COL-1 content to achieve anti-aging effects. The effect of Example 3 group is the best, and under the same dosage conditions, it is better than the positive drug group and other Chinese medicine composition groups.

[0234] The above description of the disclosed embodiments will enable one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be readily apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not limited to the embodiments shown herein but is to be construed in the widest manner consistent with the principles and novel features disclosed herein.

Claims

1. A composition for resisting cell aging, characterized in that: Calculated by weight, including: The extraction raw material of the Chinese medicine extract A is Centella asiatica; The extraction raw material of the Chinese medicine extract B is ginseng; The extraction raw materials of the Chinese medicine extract C include Zanthoxylum bungeanum and ginger root; The extraction raw materials of the traditional Chinese medicine extract D include Gentiana macrophylla and Gentiana scabra.

2. The anti-cell aging composition according to claim 1, characterized in that The composition comprises, by weight:

3. The anti-cell aging composition according to claim 1 or 2, characterized in that The extraction raw materials of the traditional Chinese medicine extract C include 1 part of Zanthoxylum bungeanum and 1 to 3 parts of ginger root.

4. The anti-cellular aging composition according to any one of claims 1 to 3, characterized in that The extraction raw materials of the traditional Chinese medicine extract D include 1 part of Gentiana macrophylla and 0.8-2 parts of Gentiana scabra.

5. The anti-cellular aging composition according to any one of claims 1 to 4, characterized in that The Chinese medicine extract A is obtained by extracting Centella asiatica with carbon dioxide in the presence of an entrainer; The extraction conditions are set as follows: temperature is 50-80°C, pressure is 15-30 MPa; The entrainer is 40-50 wt% ethanol; The extraction is performed 1 to 3 times, and the extraction further comprises the steps of concentration and drying.

6. The anti-cellular aging composition according to any one of claims 1 to 5, characterized in that The Chinese medicine extract B is obtained by ultrasonic extraction of a mixture of ginseng and a solvent; The solid-liquid ratio of the ginseng to the solvent is 1:20 to 1:35 g / mL; The solvent is 60-80 wt% ethanol; The extraction is performed 1 to 3 times, and the extraction further comprises the steps of concentration and drying.

7. The anti-cellular aging composition according to any one of claims 1 to 6, characterized in that The Chinese herbal extract C is obtained by steam distilling a mixture of Zanthoxylum bungeanum, ginger root and water to extract volatile oil, and then drying the volatile oil; The ratio of the total mass of the pepper and ginger root to the mass of water is 1:(10-15).

8. The anti-cellular aging composition according to any one of claims 1 to 7, characterized in that The Chinese medicine extract D is obtained by flash extraction of a mixture of Gentiana macrophylla, Gentiana scabra and a solvent; The extraction conditions are set as follows: extraction temperature 55-70°C, extraction voltage 110-120V, and extraction time 60-120s; The solvent is 40-60 wt% ethanol, and the ratio of the total mass of the gentiana macrophylla and gentiana macrophylla to the volume of the solvent is 1 g: (50-70) mL; The extraction is performed 1 to 3 times, and the extraction further comprises the steps of concentration and drying.

9. A product for resisting cell aging, characterized in that: A composition for resisting cell aging according to any one of claims 1 to 8.

10. The product according to claim 9, characterized in that The products include serums, lotions or creams.

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