Immunoregulation, antioxidation and sleep-aiding compound composition and preparation method thereof

Abstract

The invention relates to an immunoregulation, antioxidation and sleep-aiding compound composition and a preparation method thereof.The preparation method comprises the steps that astragalus membranaceus and acanthopanax senticosus are treated through a subcritical water extraction technology, the extraction conditions are that the temperature is 120 DEG C and the pressure is 2 MPa, and an extract rich in saponin and polysaccharide is obtained; chinese wolfberry fruits and cranberries are treated by adopting a composite enzymolysis technology, and cellulase and pectinase are used for enzymolysis, so that the dissolution rate of polysaccharides is increased; the ganoderma lucidum spore powder is subjected to superfine grinding and low-temperature wall breaking treatment, and the wall breaking rate is larger than or equal to 98% According to the immunoregulation, anti-oxidation and sleep-aiding compound composition and the preparation method thereof, the synergistic effect of three effects of immunoregulation, anti-oxidation and sleep improvement is achieved, and all the active ingredients achieve synergistic interaction through different mechanisms: the acanthopanax senticosus polysaccharide and the ganoderma lucidum beta-glucan activate inherent immunity, the astragaloside and the lycium barbarum polysaccharide enhance adaptive immunity, and the anti-oxidation and sleep-aiding compound composition has the effects of improving immunity, resisting oxidation and aiding sleep. While the sleep is improved, the cortisol level is reduced, and the vicious circle of stress, insomnia and immunity decline is blocked.

Description

Technical Field

[0001] The present invention relates to the technical field of medical care, and in particular to an immunomodulatory, antioxidant and sleep-aiding compound composition and a preparation method thereof. Background Art

[0002] With the accelerated pace of life and increased work pressure in modern society, problems such as decreased immunity, oxidative stress and sleep disorders have become increasingly prominent and have become the main issues affecting the health of modern people.

[0003] Currently, there are a wide variety of health care products on the market that target immune regulation, anti-oxidation, or sleep improvement. For example, Chinese patent 202411491998.X proposes a composition with immune regulation function and its preparation method. This composition is derived from the effective ingredients of traditional Chinese medicine that are both medicinal and edible, and can be used in susceptible populations with immune imbalances, and to improve inflammatory responses such as sore throats.

[0004] However, the following technical defects are common: First, most products have a single function, targeting only one of the functions of immune regulation, anti-oxidation or sleep aid, which makes it difficult to meet consumers' demand for complex functional health products; second, traditional Chinese herbal medicine formulas often use simple water extraction or alcohol extraction processes, which have low extraction efficiency of active ingredients and lack effective protection technology, resulting in unstable content of active ingredients in the products and low bioavailability; third, the existing technology for processing precious raw materials such as Ganoderma lucidum spore powder is not perfect, and conventional wall-breaking methods are prone to cause loss of active ingredients, affecting the efficacy of the final product.

[0005] Therefore, a composite composition of immunomodulatory, antioxidant and sleep-inducing medicine and its preparation method are developed to solve the above problems. Summary of the Invention

[0006] In response to the deficiencies in the prior art, the present invention provides a compound composition for immunoregulation, antioxidant and sleep-aiding, and a preparation method thereof. The composition has the advantages of immunoregulation, antioxidant and sleep-aiding, and has the advantages of high active ingredient retention rate and good stability. It solves the problem that most health care products for immunoregulation, antioxidant or sleep improvement on the market currently have single functions, only targeting one of the functions of immunoregulation, antioxidant or sleep-aiding, and are difficult to meet consumers' demand for complex functional health care products; secondly, traditional Chinese herbal medicine formulas often use simple water extraction or alcohol extraction processes, which have low extraction efficiency of active ingredients and lack effective protection technology, resulting in unstable content of active ingredients in the product and low bioavailability; thirdly, the processing technology for precious raw materials such as Ganoderma lucidum spore powder in the prior art is not perfect, and conventional wall-breaking methods are prone to cause loss of active ingredients, affecting the efficacy of the final product.

[0007] To achieve the above objectives, the present invention provides the following technical solution: an immunomodulatory, antioxidant and sleep-inducing compound composition, comprising the following medicinal and edible ingredients in the following weight percentages: 15-25% of Acanthopanax senticosus, 10-20% of Astragalus, 8-15% of Lycium barbarum, 10-25% of Cranberry, 8-12% of Ziziphus jujuba seed, 5-10% of broken-wall Ganoderma lucidum spore powder, 5-10% of Chinese yam, 3-8% of Poria cocos, and 2-5% of Licorice.

[0008] Preferably, the particle size of the broken spore powder of Ganoderma lucidum is ≤5 μm, the wall-breaking rate is ≥98%, and the total content of immunomodulatory active ingredients in the composition is ≥60%.

[0009] Preferably, the composition is prepared in the form of prepared granules, soft capsules or fermented dairy products.

[0010] Preferably, the weight ratio of Acanthopanax senticosus to Astragalus membranaceus is 1:(0.5-1.5), and the weight ratio of Lycium barbarum to Cranberry is 1:(1-2).

[0011] A method for preparing a compound composition comprises the following steps:

[0012] (1) Astragalus and Acanthopanax senticosus were treated with subcritical water extraction technology at a temperature of 120°C and a pressure of 2 MPa to obtain extracts rich in saponins and polysaccharides;

[0013] (2) Using composite enzymatic hydrolysis technology to treat wolfberry and cranberry, using cellulase and pectinase for enzymatic hydrolysis to improve the polysaccharide dissolution rate;

[0014] (3) Ganoderma lucidum spore powder is subjected to ultrafine grinding and low-temperature wall-breaking treatment, with a wall-breaking rate of ≥98%;

[0015] (4) mixing the broken-wall Ganoderma lucidum spore powder obtained in step (3) with a sodium alginate-chitosan solution, and preparing microencapsulated spore powder by spray drying;

[0016] (5) The extracts and microencapsulated spore powder obtained in steps (1), (2) and (4) are mixed with other ingredients in proportion to prepare a target dosage form.

[0017] Preferably, in the microencapsulation process, the mass ratio of sodium alginate to chitosan is (1-3):1.

[0018] Preferably, in the composite enzymatic hydrolysis process, the enzymatic hydrolysis temperature is 45-55° C., and the enzymatic hydrolysis time is 2-4 hours.

[0019] Preferably, the subcritical water extraction time is 1-3 hours, and the embedding rate of the microencapsulated spore powder is ≥90%.

[0020] Preferably, the air inlet temperature of the spray drying process is 160-180°C, and the air outlet temperature is 80-90°C.

[0021] Compared with the existing technology, the technical solution of this application has the following beneficial effects:

[0022] 1. This immunomodulatory, antioxidant and sleep-aiding compound composition and its preparation method achieve the synergistic effect of triple efficacy of immunomodulation, antioxidant and sleep improvement by scientifically combining multiple medicinal and edible ingredients such as Acanthopanax senticosus, Astragalus, and Ganoderma lucidum spore powder. Each active ingredient synergizes through different mechanisms: Acanthopanax senticosus polysaccharide and Ganoderma lucidum β-glucan activate innate immunity, Astragalus saponin and Lycium barbarum polysaccharide enhance adaptive immunity, Chinese yam polysaccharide and cranberry polyphenol strengthen mucosal immunity, forming a complete immune protection network. Ziziphus jujuba seed and Ganoderma lucidum triterpenoids synergistically regulate the nervous system, reducing cortisol levels while improving sleep, and blocking the vicious cycle of stress, insomnia and decreased immunity.

[0023] 2. This immunomodulatory, antioxidant and sleep-aiding compound composition and its preparation method use subcritical water extraction technology to achieve the simultaneous and efficient extraction of saponins and polysaccharides from astragalus and Acanthopanax, improving the extraction efficiency by more than 30%. It innovatively applies composite enzymatic hydrolysis technology to the extraction of wolfberry and cranberry, and through the synergistic action of cellulase and pectinase, the polysaccharide dissolution rate is increased to more than 92%. The developed ultrafine grinding combined with low-temperature wall-breaking process enables the wall-breaking rate of Ganoderma lucidum spore powder to reach more than 98%, and the active ingredient loss rate is less than 5%. The sodium alginate-chitosan microencapsulation technology enables the protection rate of Ganoderma lucidum spore powder in simulated gastric fluid to reach more than 90%, realizing intestinal targeted release. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a structural schematic diagram of the present invention. DETAILED DESCRIPTION

[0025] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0026] Example 1

[0027] See also Figure 1The immunomodulatory, antioxidant and sleep-aiding compound composition in this embodiment is composed of the following medicinal and edible ingredients in the following weight percentages: 15% of Acanthopanax senticosus, 10% of Astragalus, 8% of Lycium barbarum, 10% of Cranberry, 8% of Ziziphus jujuba seed, 5% of broken-wall Ganoderma lucidum spore powder, 5% of Dioscorea opposita, 3% of Poria cocos, and 2% of Licorice. The particle size of the broken-wall Ganoderma lucidum spore powder is 3 μm, and the wall-breaking rate is 98%. The total content of immunomodulatory active ingredients in the composition is 60%. The composition is prepared in the form of instant granules, soft capsules or fermented dairy products. The weight ratio of Acanthopanax senticosus to Astragalus is 1:0.5, and the weight ratio of Lycium barbarum to cranberry is 1:1.

[0028] A method for preparing a compound composition comprises the following steps:

[0029] (1) Subcritical water extraction technology was used to treat Astragalus and Acanthopanax senticosus. The extraction conditions were a temperature of 120°C and a pressure of 2 MPa. Extracts rich in saponins and polysaccharides were obtained. The subcritical water extraction time was 1 hour, and the encapsulation efficiency of the microencapsulated spore powder was 90%;

[0030] (2) Using composite enzymatic hydrolysis technology to treat wolfberry and cranberry, using cellulase and pectinase for enzymatic hydrolysis to improve the polysaccharide dissolution rate;

[0031] (3) Ganoderma lucidum spore powder was subjected to ultrafine grinding and low-temperature wall-breaking treatment, with a wall-breaking rate of 98%;

[0032] (4) mixing the broken-wall Ganoderma lucidum spore powder obtained in step (3) with a sodium alginate-chitosan solution, and preparing microencapsulated spore powder by spray drying;

[0033] (5) The extracts and microencapsulated spore powder obtained in steps (1), (2) and (4) are mixed with other ingredients in proportion to prepare a target dosage form.

[0034] Specifically, in the microencapsulation process, the mass ratio of sodium alginate to chitosan is 1:1; in the composite enzymatic hydrolysis process, the enzymatic hydrolysis temperature is 45°C, the enzymatic hydrolysis time is 2 hours; and in the spray drying process, the inlet air temperature is 160°C, and the outlet air temperature is 80°C.

[0035] In this example, the extracts, microencapsulated spore powder and auxiliary materials were uniformly mixed, granulated by a wet method, dried at 60° C., granulated and then packaged to prepare instant granules.

[0036] Example 2

[0037] See also Figure 1The immunomodulatory, antioxidant and sleep-aiding compound composition in this embodiment is composed of the following medicinal and edible ingredients in the following weight percentages: 25% of Acanthopanax senticosus, 20% of Astragalus, 15% of Lycium barbarum, 25% of Cranberry, 12% of Ziziphus jujuba seed, 10% of broken-wall Ganoderma lucidum spore powder, 10% of Dioscorea opposita, 8% of Poria cocos, and 5% of Licorice. The particle size of the broken-wall Ganoderma lucidum spore powder is 5 μm, and the wall-breaking rate is 99%. The total content of immunomodulatory active ingredients in the composition is 65%. The composition is prepared in the form of instant granules, soft capsules or fermented dairy products. The weight ratio of Acanthopanax senticosus to Astragalus is 1:1.5, and the weight ratio of Lycium barbarum to cranberry is 1:2.

[0038] A method for preparing a compound composition comprises the following steps:

[0039] (1) Subcritical water extraction technology was used to treat Astragalus and Acanthopanax senticosus. The extraction conditions were temperature 120°C and pressure 2 MPa. Extracts rich in saponins and polysaccharides were obtained. The subcritical water extraction time was 3 hours, and the embedding efficiency of microencapsulated spore powder was 95%;

[0040] (2) Using composite enzymatic hydrolysis technology to treat wolfberry and cranberry, using cellulase and pectinase for enzymatic hydrolysis to improve the polysaccharide dissolution rate;

[0041] (3) Ganoderma lucidum spore powder was subjected to ultrafine grinding and low-temperature wall-breaking treatment, with a wall-breaking rate of 99%;

[0042] (4) mixing the broken-wall Ganoderma lucidum spore powder obtained in step (3) with a sodium alginate-chitosan solution, and preparing microencapsulated spore powder by spray drying;

[0043] (5) The extracts and microencapsulated spore powder obtained in steps (1), (2) and (4) are mixed with other ingredients in proportion to prepare a target dosage form.

[0044] Specifically, in the microencapsulation process, the mass ratio of sodium alginate to chitosan is 3:1; in the composite enzymatic hydrolysis process, the enzymatic hydrolysis temperature is 55°C, the enzymatic hydrolysis time is 4 hours; and in the spray drying process, the inlet air temperature is 180°C, and the outlet air temperature is 90°C.

[0045] In this embodiment, 10 g of wolfberry extract was taken, 50 g of soybean oil and 3 g of lecithin were added, and the mixture was stirred and dissolved at 60 ° C. 20 g of the astragalus-acanthopanax extract prepared in this embodiment was taken, 15 g of cranberry extract and 10 g of jujube seed extract were added, 100 mL of purified water was added, and the mixture was homogenized. The oil phase was slowly added to the aqueous phase, and the mixture was emulsified at 8000 rpm for 10 minutes. The mixture was sterilized by a 0.45 μm microporous filter membrane, and a gelatin-glycerol capsule material was used. The filling temperature was 40 ° C. The filling amount per capsule was 500 mg, and the soft capsule product was obtained after drying.

[0046] Example 3

[0047] See also Figure 1 The immunomodulatory, antioxidant and sleep-aiding compound composition in this embodiment is composed of the following medicinal and edible ingredients in the following weight percentages: 20% of Acanthopanax senticosus, 15% of Astragalus, 10% of Lycium barbarum, 20% of Cranberry, 10% of Ziziphus jujuba seed, 8% of broken-wall Ganoderma lucidum spore powder, 7% of Dioscorea opposita, 5% of Poria cocos, and 3% of Licorice. The particle size of the broken-wall Ganoderma lucidum spore powder is 3 μm, and the wall-breaking rate is 98%. The total content of immunomodulatory active ingredients in the composition is 63%. The composition is prepared in the form of instant granules, soft capsules or fermented dairy products. The weight ratio of Acanthopanax senticosus to Astragalus is 1:0.9, and the weight ratio of Lycium barbarum to cranberry is 1:1.5.

[0048] A method for preparing a compound composition comprises the following steps:

[0049] (1) Astragalus and Acanthopanax senticosus were treated with subcritical water extraction technology at a temperature of 120°C and a pressure of 2 MPa to obtain extracts rich in saponins and polysaccharides. The subcritical water extraction time was 2 hours, and the encapsulation efficiency of the microencapsulated spore powder was 92%;

[0050] (2) Using composite enzymatic hydrolysis technology to treat wolfberry and cranberry, using cellulase and pectinase for enzymatic hydrolysis to improve the polysaccharide dissolution rate;

[0051] (3) Ganoderma lucidum spore powder is subjected to ultrafine grinding and low-temperature wall-breaking treatment, with a wall-breaking rate of ≥98%;

[0052] (4) mixing the broken-wall Ganoderma lucidum spore powder obtained in step (3) with a sodium alginate-chitosan solution, and preparing microencapsulated spore powder by spray drying;

[0053] (5) The extracts and microencapsulated spore powder obtained in steps (1), (2) and (4) are mixed with other ingredients in proportion to prepare a target dosage form.

[0054] Specifically, in the microencapsulation process, the mass ratio of sodium alginate to chitosan is 2:1; in the composite enzymatic hydrolysis process, the enzymatic hydrolysis temperature is 50°C, the enzymatic hydrolysis time is 3 hours; and in the spray drying process, the inlet air temperature is 175°C, and the outlet air temperature is 86°C.

[0055] In this embodiment, 1000 g of fresh milk was taken, 150 g of cranberry juice, 5 g of Poria cocos extract, and 6 g of yam extract were added. After homogenization, the mixture was sterilized at 95° C. for 5 minutes, cooled to 40° C., inoculated with 3% Lactobacillus bulgaricus and Streptococcus thermophilus (1:1), fermented at 42° C. for 6 hours to a pH of 4.6, 8 g of the microencapsulated Ganoderma lucidum spore powder prepared in Example 1 was added, and the mixture was post-ripened at 4° C. for 24 hours and aseptically filled to obtain functional fermented milk.

[0056] Example 4

[0057] In this example, the products of Examples 1-3 were placed under conditions of 40°C ± 2°C and RH 75% ± 5%, and samples were taken for testing at 0, 1, 2, 3, and 6 months, respectively.

[0058] The retention rates of the main active ingredients are shown in the following table:

[0059] time Acanthopanax senticosus saponins Astragaloside IV Lycium barbarum polysaccharides Ganoderma lucidum triterpenes October 100％ 100％ 100％ 100％ March 93.2％ 91.5％ 95.7％ 89.8％ June 86.7％ 85.2％ 90.3％ 82.4％

[0060] Experimental Example 1: Evaluation of Immunomodulatory Function

[0061] 1) Experimental Materials and Methods

[0062] Experimental animals: BALB / c male mice, 6-8 weeks old, were randomly divided into 5 groups (n=10).

[0063] Control groups: normal control group, model control group (cyclophosphamide 80 mg / kg), positive control group (transfer factor), low-dose group (0.5 g / kg of the composition of the present invention), and high-dose group (1.0 g / kg of the composition of the present invention).

[0064] Administration: Oral administration, for 14 consecutive days.

[0065] Detection indicators: organ index (thymus, spleen), serum immunoglobulins (IgG, IgA, IgM), lymphocyte subsets (CD4+ / CD8+ ratio), and cytokines (IL-2, IFN-γ).

[0066] 2) Experimental results

[0067] The effects on immune organs are shown in the following table:

[0068] Group Thymus index (mg / g) Spleen index (mg / g) Normal control 3.12±0.25 5.45±0.38 Model comparison 1.85±0.18 3.02±0.26 Positive control 2.76±0.21 4.83±0.32 Low-dose group 2.53±0.19 4.25±0.29 High-dose group 2.94±0.22 5.18±0.35

[0069] The effects on humoral immunity are shown in the following table:

[0070] Group IgG (mg / mL) IgA (μg / mL) IgM (μg / mL) Normal control 12.35±1.02 245.6±18.7 156.8±12.3 Model comparison 6.82±0.75 132.5±10.2 85.4±7.6 High-dose group 11.28±0.98 221.8±17.5 142.6±11.8

[0071] Experimental Example 2: Evaluation of Antioxidant Activity

[0072] 1) In vitro antioxidant test

[0073] DPPH free radical scavenging experiment: IC50 value was 0.38 mg / mL for the composition of the present invention vs 0.12 mg / mL for VC.

[0074] Hydroxyl free radical scavenging experiment: The scavenging rate of 1 mg / mL sample reached 65.2%, and the total antioxidant capacity (FRAP method) was equivalent to 0.85 mmol FeSO4 / g sample.

[0075] 2) In vivo antioxidant experiment:

[0076] Aging model mice (D-galactose induced) are as follows:

[0077] index Model Group The present invention group Normal group SOD(U / mgprot) 68.5±5.2 112.3±8.7 125.6±9.3 MDA (nmol / mg) 8.76±0.65 4.32±0.38 3.85±0.31 GSH-Px (U / mg) 35.2±2.8 62.5±4.9 68.3±5.1

[0078] Experimental Example 3: Evaluation of sleep improvement function

[0079] 1) Direct sleep experiment

[0080] The details of the subthreshold dose synergy experiment of pentobarbital sodium are as follows:

[0081] Group Sleeping rate (%) Sleep time (min) Blank control 20 - Positive control 80 68.5±7.2 The present invention group 75 62.3±6.8

[0082] 2) Sleep quality evaluation

[0083] Clinical observation (n=60), specific data are as follows:

[0084] index Before taking the medicine After 4 weeks of medication Sleep latency (min) 45.6±12.3 23.8±8.7 Sleep efficiency (%) 72.5±9.8 88.3±7.5 PSQI total score 10.8±2.5 6.2±1.8

[0085] Experimental Example 4: Acute toxicity test

[0086] 1) Experimental methods

[0087] ICR mice were selected for the maximum dosage test, with a single oral administration of 20 g / kg (maximum feasible dose) and observation for 14 days.

[0088] Results: No animals died, body weight increased normally, and no abnormalities were found in the pathological examination of major organs. LD50 was > 20 g / kg, which is practically non-toxic.

[0089] 2) 30-day feeding trial

[0090] Experimental design: SD rats were enrolled in three dose groups: 0.5, 1.0, and 2.0 g / kg. The drugs were administered for 30 consecutive days. Blood routine, blood biochemistry, organ coefficients, and tissue pathology were measured.

[0091] result

[0092] Each dose group: There was no significant difference in weight gain compared with the control group; blood routine and liver and kidney function indicators were all within the normal range; no pathological changes were found in the major organs; the NOAEL was 2.0g / kg.

[0093] In summary, the immunomodulatory, antioxidant and sleep-aiding compound composition and its preparation method achieve the synergistic effect of the triple effects of immunomodulation, antioxidant and sleep improvement by scientifically combining multiple medicinal and edible ingredients such as Acanthopanax senticosus, Astragalus, Ganoderma lucidum spore powder, etc. The active ingredients synergize through different mechanisms: Acanthopanax senticosus polysaccharides and Ganoderma lucidum β-glucan activate innate immunity, Astragalus saponins and Lycium barbarum polysaccharides enhance adaptive immunity, Chinese yam polysaccharides and cranberry polyphenols strengthen mucosal immunity, forming a complete immune protection network, and Chinese jujube seeds and Ganoderma lucidum triterpenes synergistically regulate the nervous system, reducing cortisol levels while improving sleep, and blocking the vicious cycle of stress, insomnia and decreased immunity. Subcritical water extraction technology is used to achieve simultaneous and efficient extraction of saponins and polysaccharides from Astragalus and Acanthopanax, with the extraction efficiency increased by more than 30%. The composite enzymatic hydrolysis technology is innovatively applied to the extraction of wolfberry and cranberry. Through the synergistic action of cellulase and pectinase, the polysaccharide dissolution rate is increased to more than 92%. The developed ultrafine grinding combined with low-temperature wall-breaking process enables the wall-breaking rate of Ganoderma lucidum spore powder to reach more than 98%, and the active ingredient loss rate is less than 5%. The sodium alginate-chitosan microencapsulation technology enables the protection rate of Ganoderma lucidum spore powder in simulated gastric fluid to reach more than 90%, realizing intestinal targeted release.

[0094] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply the existence of any such actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article, or device comprising a series of elements includes not only those elements, but also other elements not explicitly listed, or elements inherent to such process, method, article, or device. In the absence of further limitations, an element defined by the phrase "comprising a ..." does not exclude the presence of other identical elements in the process, method, article, or device comprising the element.

[0095] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. An immunomodulatory, antioxidant and sleep-inducing compound composition, characterized in that: The medicine is composed of the following medicinal and edible ingredients in the following weight percentages: 15-25% of Acanthopanax senticosus, 10-20% of Astragalus, 8-15% of Lycium barbarum, 10-25% of Cranberry, 8-12% of Ziziphus jujuba seeds, 5-10% of broken wall Ganoderma lucidum spore powder, 5-10% of Chinese yam, 3-8% of Poria cocos and 2-5% of Licorice.

2. The immunomodulatory, antioxidant and sleep-aiding compound composition according to claim 1, characterized in that: The particle size of the broken wall-ganoderma spore powder is ≤5 μm, the wall-breaking rate is ≥98%, and the total content of the immunomodulatory active ingredients in the composition is ≥60%.

3. The immunomodulatory, antioxidant and sleep-aiding compound composition according to claim 1, characterized in that: The composition is prepared in the form of prepared granules, soft capsules or fermented dairy products.

4. The immunomodulatory, antioxidant and sleep-aiding compound composition according to claim 1, characterized in that: The weight ratio of the Acanthopanax senticosus to the Astragalus membranaceus is 1:(0.5-1.5), and the weight ratio of the Lycium barbarum to the Cranberry is 1:(1-2).

5. A method for preparing the compound composition according to any one of claims 1 to 4, characterized in that: The following steps are involved: (1) Astragalus and Acanthopanax senticosus were treated with subcritical water extraction technology at a temperature of 120°C and a pressure of 2 MPa to obtain extracts rich in saponins and polysaccharides; (2) Using composite enzymatic hydrolysis technology to treat wolfberry and cranberry, using cellulase and pectinase for enzymatic hydrolysis to improve the polysaccharide dissolution rate; (3) Ganoderma lucidum spore powder is subjected to ultrafine grinding and low-temperature wall-breaking treatment, with a wall-breaking rate of ≥98%; (4) mixing the broken-wall Ganoderma lucidum spore powder obtained in step (3) with a sodium alginate-chitosan solution, and preparing microencapsulated spore powder by spray drying; (5) The extracts and microencapsulated spore powder obtained in steps (1), (2) and (4) are mixed with other ingredients in proportion to prepare a target dosage form.

6. The method for preparing the immunomodulatory, antioxidant and sleep-aiding compound composition according to claim 6, characterized in that: In the microencapsulation process, the mass ratio of sodium alginate to chitosan is (1-3):

1.

7. The method for preparing the immunomodulatory, antioxidant and sleep-aiding compound composition according to claim 6, characterized in that: In the composite enzymatic hydrolysis process, the enzymatic hydrolysis temperature is 45-55° C., and the enzymatic hydrolysis time is 2-4 hours.

8. The method for preparing the immunomodulatory, antioxidant and sleep-aiding compound composition according to claim 6, characterized in that: The subcritical water extraction time is 1-3 hours, and the embedding rate of the microencapsulated spore powder is ≥90%.

9. The method for preparing the immunomodulatory, antioxidant and sleep-aiding compound composition according to claim 6, characterized in that: The inlet air temperature of the spray drying process is 160-180°C, and the outlet air temperature is 80-90°C.

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