Extraction process and application of herbaceous plant combination capable of replenishing qi and enriching blood

Abstract

The invention belongs to the technical field of traditional Chinese medicine extraction, and particularly relates to an extraction process and application of a qi-tonifying and blood-enriching herbaceous plant combination. According to the present invention, the medium-short infrared high-temperature irradiation and gradient pressurization extraction process is adopted, the carbon dioxide and the nitrogen are assisted, the active components in the raw material are efficiently extracted and stabilized, the method is the efficient and low-loss integrated process, the extraction is performed simultaneously, the preparation period is shortened, and the method is particularly suitable for industrial production.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicine extraction, and particularly relates to a combined extraction process of qi-invigorating and blood-tonifying herbal plants and its application. Background Art

[0002] The Yellow Emperor's Classic of Internal Medicine states, "A person possesses only blood and qi." Traditional Chinese medicine states, "Blood is the mother of qi, and qi is the commander of blood." This suggests that qi and blood are interdependent, mutually reinforcing and accompanying each other, jointly maintaining and promoting life. "Blood Syndrome Treatise: Yin and Yang, Water, Fire, Blood, and Qi" states, "Blood is the guardian of qi." "Lingshu: Yingsheng Huipian" states, "Ying circulates in the meridians," referring to the presence of Yingqi within the blood vessels. Qi circulates because blood carries qi. If qi were not stored in the blood, it would dissipate and have nowhere to return. Qi resides in the blood, carrying qi and continuously providing it with the essence of water and grain, nourishing it for its functions. Therefore, when qi is abundant, blood flourishes, while blood deficiency leads to qi decline. Loss of blood leads to loss of qi, which in turn leads to blood and qi deficiency. Consequently, clinically, patients with blood deficiency often experience symptoms of qi deficiency, such as shortness of breath, fatigue, and laziness.

[0003] Because Qi and blood are closely interdependent, mutually reinforcing, and mutually beneficial, they often influence each other when pathological changes occur. Qi and blood deficiency refers to the simultaneous presence of both Qi and blood deficiency symptoms.

[0004] With the change of modern lifestyle and the surge of social pressure, there are a large number of sub-healthy groups with symptoms of Qi and blood deficiency among many young and middle-aged people. However, the Qi and blood deficiency shown by this group of people is not due to the lack of Qi and blood biochemical source caused by insufficient nutrients, but due to the insufficient transportation of grains caused by an improper lifestyle. In addition, long-term exposure to air-conditioned environments, long periods of sitting and little exercise and other unfavorable factors affect the normal transportation of Qi and blood in the body. Therefore, it is proposed to replenish Qi and blood, supplemented by promoting Qi circulation and removing stagnation, in order to promote the circulation of Qi and blood, increase the nourishing power of blood and the warming power of Qi, so as to mobilize the body's Qi and blood transportation to normal, enable the essence of grains to fully nourish the limbs, and the body to become more peaceful. For this type of people with Qi deficiency and blood stasis, relatively mild dual-purpose medicinal and edible ingredients are selected, and through reasonable formulation, the effect of diet therapy is achieved. Therefore, the King of Medicine Sun Simiao said in "Qianjin Yaofang": "Those who do not know what to eat are not able to live a full life", "If the king or father is ill, he should first prescribe food to treat it. If the diet therapy does not work, then prescribe medicine." Currently, traditional extraction methods such as water extraction and alcohol extraction have the problems of high destruction rate of active ingredients and low extraction efficiency. The raw materials are easily affected by light, heat, and oxygen, leading to degradation and poor storage stability. In addition, a single process is difficult to take into account the simultaneous retention and functional synergy of multiple types of active ingredients. Summary of the Invention

[0005] The present invention provides a combined extraction process of herbal plants for replenishing qi and blood. The extraction process of the present invention adopts a mixed extraction method of traditional Chinese medicine and adopts the following technical scheme: A combined extraction process of herbal plants for replenishing qi and blood, comprising the following steps: (1) The prescribed amount of the herbal combination of invigorating qi and nourishing blood is crushed and then processed using medium and short wave infrared equipment; (2) Mixing the raw material powder with water, placing it in a reaction vessel, adjusting the pressure and temperature, and flushing carbon dioxide into the vessel during the extraction process; after the extraction is completed, filtering, and collecting the filtrate; (3) Mix the filter residue obtained in step 2 with water, place it in a reaction vessel, adjust the pressure and temperature, and flush nitrogen into the vessel during the extraction process; after the extraction is completed, filter and collect the filtrate; (4) Mix the filter residue obtained in step 3 with water, place it in a reaction vessel, adjust the pressure and temperature, and flush nitrogen into the vessel during the extraction process; after the extraction is completed, filter and collect the filtrate; (5) The filtrates obtained in steps 2-4 are mixed and concentrated to obtain an extract.

[0006] Preferably, the particle size of the raw material crushed in step (1) is 1-10 μm.

[0007] Preferably, the medium- and short-wave infrared equipment treatment conditions in step (1) are 120-150° C. for 20-30 min.

[0008] Preferably, in step (2), the raw material powder and water are mixed in a mass ratio of 1:8-10, at a pressure of 0.02-0.08 MPa, a temperature of 50-60° C., and a time of 2-3 h.

[0009] Preferably, in step (3), the filter residue and water are mixed in a mass ratio of 1:6-8, at a pressure of 150-200 MPa, a temperature of 80-100° C., and a time of 1-2 h.

[0010] Preferably, in step (4), the filter residue and water are mixed in a mass ratio of 1:4-6, at a pressure of 300-400 MPa, a temperature of 120-140° C., and a time of 0.5-1 h.

[0011] In a preferred embodiment, a combined extraction process of qi-invigorating and blood-tonifying herbal plants comprises the following steps: (1) The prescribed amount of raw materials is crushed to a particle size of 1-10 μm, and then treated with medium- and short-wave infrared equipment at 120-150°C for 20-30 minutes; (2) Mix the raw material powder and water in a mass ratio of 1:8-10, place in a reaction vessel, pressure 0.02-0.08 MPa, temperature 50-60°C, time 2-3 hours, flush carbon dioxide into the vessel during the extraction process; after the extraction is completed, filter and collect the filtrate; (3) Mix the filter residue obtained in step 2 with water in a mass ratio of 1:6-8, place it in a reaction vessel, and incubate at a pressure of 150-200 MPa, a temperature of 80-100°C, and a time of 1-2 h. During the extraction process, flush nitrogen into the vessel. After the extraction is completed, filter and collect the filtrate. (4) The filter residue obtained in step 3 was mixed with water in a mass ratio of 1:4-6, placed in a reaction vessel, and subjected to a pressure of 300-400 MPa, a temperature of 120-140°C, and a time of 0.5-1 h. During the extraction process, nitrogen was flushed into the vessel. After the extraction was completed, the filtrate was filtered and collected; (5) The filtrates obtained in steps 2-4 are mixed and concentrated to obtain an extract.

[0012] In a preferred embodiment, the herbal combination for invigorating qi and nourishing blood comprises Rehmannia root, donkey-hide gelatin, astragalus root, Codonopsis pilosula, red dates, Chinese yam, longan, Chinese angelica, ginseng, hawthorn, and liquorice.

[0013] Preferably, the combination comprises 18-22 parts of Rehmannia root, 8-12 parts of donkey-hide gelatin, 16-20 parts of Astragalus root, 8-12 parts of Codonopsis pilosula, 8-12 parts of red dates, 6-10 parts of Chinese yam, 6-10 parts of longan, 10-14 parts of Chinese angelica, 4-8 parts of ginseng, 3-7 parts of hawthorn, and 2-6 parts of liquorice.

[0014] Further preferably, the combination comprises 20 parts of Rehmannia root, 10 parts of donkey-hide gelatin, 18 parts of Astragalus root, 10 parts of Codonopsis pilosula, 10 parts of red dates, 8 parts of Chinese yam, 8 parts of longan, 12 parts of Chinese angelica, 6 parts of ginseng, 5 parts of hawthorn, and 4 parts of liquorice.

[0015] In a preferred embodiment, a combined extraction process of qi-invigorating and blood-tonifying herbal plants comprises the following steps: (1) According to the prescription amount, weigh the raw materials of each component of the combination, 15-25 parts of Rehmannia root, 5-15 parts of donkey-hide gelatin, 15-25 parts of Astragalus root, 5-15 parts of Codonopsis pilosula, 5-15 parts of red dates, 4-12 parts of Chinese yam, 4-12 parts of longan, 8-16 parts of Chinese angelica, 2-10 parts of ginseng, 2-10 parts of hawthorn, and 1-8 parts of liquorice, mix them and grind them into particles of 1-10 μm, and then treat them with medium- and short-wave infrared equipment at 120-150°C for 20-30 minutes; (2) Mix the raw material powder and water in a mass ratio of 1:8-10, place in a reaction vessel, pressure 0.02-0.08 MPa, temperature 50-60°C, time 2-3 hours, flush carbon dioxide into the vessel during the extraction process; after the extraction is completed, filter and collect the filtrate; (3) Mix the filter residue obtained in step 2 with water in a mass ratio of 1:6-8, place it in a reaction vessel, and incubate at a pressure of 150-200 MPa, a temperature of 80-100°C, and a time of 1-2 h. During the extraction process, flush nitrogen into the vessel. After the extraction is completed, filter and collect the filtrate. (4) The filter residue obtained in step 3 was mixed with water in a mass ratio of 1:4-6, placed in a reaction vessel, and subjected to a pressure of 300-400 MPa, a temperature of 120-140°C, and a time of 0.5-1 h. During the extraction process, nitrogen was flushed into the vessel. After the extraction was completed, the filtrate was filtered and collected; (5) The filtrates obtained in steps 2-4 are mixed and concentrated to obtain an extract.

[0016] Advantages of the present invention: The present invention utilizes medium and short infrared high-temperature irradiation and gradient pressurization extraction technology, supplemented by carbon dioxide and nitrogen, to efficiently extract and stabilize the active ingredients in the raw materials. It is an efficient, low-loss integrated process that extracts at the same time, shortens the preparation cycle, and is particularly suitable for industrial production. DETAILED DESCRIPTION

[0017] The present invention will be further described below in conjunction with specific examples. It should be understood that the following examples are intended only to illustrate the present invention and are not intended to limit the scope of the present invention. Unless otherwise specified, the materials and raw materials used in the present invention can be obtained through commercial channels.

[0018] Example 1 Prescription of herbal combination for invigorating qi and nourishing blood: 200g of Rehmannia root, 100g of donkey-hide gelatin, 180g of Astragalus root, 100g of Codonopsis pilosula, 100g of red dates, 80g of Chinese yam, 80g of longan, 120g of Chinese angelica, 60g of ginseng, 50g of hawthorn, and 40g of licorice.

[0019] (1) Weigh the combined raw materials according to the prescribed amount, mix them evenly and crush them into a particle size of 5 μm, and then treat them with medium- and short-wave infrared equipment at 130°C for 25 min; (2) The raw material powder was mixed with water in a mass ratio of 1:10 and placed in a reaction vessel. The pressure was 0.08 MPa, the temperature was 60°C, and the extraction time was 3 h. During the extraction process, carbon dioxide was flushed into the vessel. After the extraction was completed, the filtrate was filtered and collected. (3) The filter residue obtained in step 2 was mixed with water in a mass ratio of 1:8, placed in a reaction vessel, and subjected to a pressure of 190 MPa, a temperature of 100°C, and a time of 1.5 h. Nitrogen was flushed into the vessel during the extraction process. After the extraction was completed, the filtrate was filtered and collected; (4) The filter residue obtained in step 3 was mixed with water in a mass ratio of 1:6, placed in a reaction vessel, and subjected to a pressure of 360 MPa, a temperature of 130°C, and a time of 1 h. During the extraction process, nitrogen was flushed into the vessel. After the extraction was completed, the filtrate was filtered and collected. (5) The filtrates obtained in steps 2-4 were mixed and concentrated to 1200 ml to obtain an extract.

[0020] Example 2 Prescription of herbal combination for invigorating qi and nourishing blood: 180g of Rehmannia root, 80g of donkey-hide gelatin, 200g of Astragalus root, 80g of Codonopsis pilosula, 120g of red dates, 60g of Chinese yam, 60g of longan, 100g of Chinese angelica, 80g of ginseng, 30g of hawthorn, and 20g of licorice.

[0021] (1) Weigh the combined raw materials according to the prescribed amount, mix them evenly and crush them into a particle size of 1 μm, and then treat them with medium- and short-wave infrared equipment at 120°C for 30 min; (2) The raw material powder was mixed with water in a mass ratio of 1:8 and placed in a reaction vessel. The pressure was 0.02 MPa, the temperature was 50°C, and the extraction time was 2 h. During the extraction process, carbon dioxide was flushed into the vessel. After the extraction was completed, the filtrate was filtered and collected. (3) The filter residue obtained in step 2 was mixed with water in a mass ratio of 1:8, placed in a reaction vessel, and subjected to a pressure of 150 MPa, a temperature of 80°C, and a time of 2 h. During the extraction process, nitrogen was flushed into the vessel. After the extraction was completed, the filtrate was filtered and collected. (4) The filter residue obtained in step 3 was mixed with water in a mass ratio of 1:4, placed in a reaction vessel, and subjected to a pressure of 300 MPa, a temperature of 120°C, and a time of 0.5 h. Nitrogen was flushed into the vessel during the extraction process. After the extraction was completed, the filtrate was filtered and collected; (5) The filtrates obtained in steps 2-4 were mixed and concentrated to 1200 ml to obtain an extract.

[0022] Example 3 Prescription of herbal combination for invigorating qi and nourishing blood: 220g of Rehmannia root, 120g of donkey-hide gelatin, 160g of Astragalus root, 120g of Codonopsis pilosula, 80g of red dates, 100g of Chinese yam, 100g of longan, 140g of Chinese angelica, 40g of ginseng, 70g of hawthorn, and 60g of licorice.

[0023] (1) Weigh the combined raw materials according to the prescribed amount, mix them evenly and crush them into a particle size of 10 μm, and then treat them with medium- and short-wave infrared equipment at 150°C for 20 min; (2) The raw material powder was mixed with water in a mass ratio of 1:9 and placed in a reaction vessel. The pressure was 0.05 MPa, the temperature was 60°C, and the extraction time was 2 h. During the extraction process, carbon dioxide was flushed into the vessel. After the extraction was completed, the filtrate was filtered and collected. (3) The filter residue obtained in step 2 was mixed with water in a mass ratio of 1:6, placed in a reaction vessel, and subjected to a pressure of 200 MPa, a temperature of 90°C, and a time of 1 h. Nitrogen was flushed into the vessel during the extraction process. After the extraction was completed, the filtrate was filtered and collected; (4) The filter residue obtained in step 3 was mixed with water in a mass ratio of 1:4, placed in a reaction vessel, and subjected to a pressure of 400 MPa, a temperature of 120°C, and a time of 0.5 h. Nitrogen was flushed into the vessel during the extraction process. After the extraction was completed, the filtrate was filtered and collected; (5) The filtrates obtained in steps 2-4 were mixed and concentrated to 1200 ml to obtain an extract.

[0024] Example 4 Prescription of herbal combination for invigorating qi and nourishing blood: 200g of Rehmannia root, 100g of donkey-hide gelatin, 180g of Astragalus root, 100g of Codonopsis pilosula, 100g of red dates, 80g of Chinese yam, 80g of longan, 120g of Chinese angelica, 60g of ginseng, 50g of hawthorn, and 40g of licorice.

[0025] (1) Weigh the combined raw materials according to the prescribed amount, mix them evenly and crush them into a particle size of 100 nm. Then use medium- and short-wave infrared equipment to treat them at 130°C for 25 min. (2) The raw material powder was mixed with water in a mass ratio of 1:10 and placed in a reaction vessel. The pressure was 0.1 MPa, the temperature was 60°C, and the extraction time was 3 h. During the extraction process, carbon dioxide was flushed into the vessel. After the extraction was completed, the filtrate was filtered and collected. (3) The filter residue obtained in step 2 was mixed with water in a mass ratio of 1:8, placed in a reaction vessel, and subjected to a pressure of 190 MPa, a temperature of 100°C, and a time of 1.5 h. Nitrogen was flushed into the vessel during the extraction process. After the extraction was completed, the filtrate was filtered and collected; (4) The filter residue obtained in step 3 was mixed with water in a mass ratio of 1:6, placed in a reaction vessel, and subjected to a pressure of 360 MPa, a temperature of 130°C, and a time of 1 h. During the extraction process, nitrogen was flushed into the vessel. After the extraction was completed, the filtrate was filtered and collected. (5) The filtrates obtained in steps 2-4 were mixed and concentrated to 1200 ml to obtain an extract.

[0026] Example 5 Prescription of herbal combination for invigorating qi and nourishing blood: 200g of Rehmannia root, 100g of donkey-hide gelatin, 180g of Astragalus root, 100g of Codonopsis pilosula, 100g of red dates, 80g of Chinese yam, 80g of longan, 120g of Chinese angelica, 60g of ginseng, 50g of hawthorn, and 40g of licorice.

[0027] (1) Weigh the combined raw materials according to the prescribed amount, mix them evenly and crush them into a particle size of 100 μm, and then treat them with medium- and short-wave infrared equipment at 130°C for 25 min; (2) The raw material powder was mixed with water in a mass ratio of 1:10 and placed in a reaction vessel. The pressure was 0.1 MPa, the temperature was 60°C, and the extraction time was 3 h. During the extraction process, carbon dioxide was flushed into the vessel. After the extraction was completed, the filtrate was filtered and collected. (3) The filter residue obtained in step 2 was mixed with water in a mass ratio of 1:8, placed in a reaction vessel, and subjected to a pressure of 190 MPa, a temperature of 100°C, and a time of 1.5 h. Nitrogen was flushed into the vessel during the extraction process. After the extraction was completed, the filtrate was filtered and collected; (4) The filter residue obtained in step 3 was mixed with water in a mass ratio of 1:6, placed in a reaction vessel, and subjected to a pressure of 360 MPa, a temperature of 130°C, and a time of 1 h. During the extraction process, nitrogen was flushed into the vessel. After the extraction was completed, the filtrate was filtered and collected. (5) The filtrates obtained in steps 2-4 were mixed and concentrated to 1200 ml to obtain an extract.

[0028] Example 6 Prescription of herbal combination for invigorating qi and nourishing blood: 200g of Rehmannia root, 100g of donkey-hide gelatin, 180g of Astragalus root, 100g of Codonopsis pilosula, 100g of red dates, 80g of Chinese yam, 80g of longan, 120g of Chinese angelica, 60g of ginseng, 50g of hawthorn, and 40g of licorice.

[0029] (1) Weigh the combined raw materials according to the prescribed amount, mix them evenly and crush them into a particle size of 5 μm, and then treat them with medium- and short-wave infrared equipment at 130°C for 25 min; (2) The raw material powder was mixed with water in a mass ratio of 1:10 and placed in a reaction vessel. The pressure was 360 MPa, the temperature was 130°C, and the extraction time was 1 h. Nitrogen was flushed into the vessel during the extraction process. After the extraction was completed, the filtrate was filtered and collected. (3) The filter residue obtained in step 2 was mixed with water in a mass ratio of 1:8, placed in a reaction vessel, and subjected to a pressure of 190 MPa, a temperature of 100°C, and a time of 1.5 h. Nitrogen was flushed into the vessel during the extraction process. After the extraction was completed, the filtrate was filtered and collected; (4) The filter residue obtained in step 3 was mixed with water in a mass ratio of 1:6, placed in a reaction vessel, and the pressure was 0.05 MPa, the temperature was 60°C, and the extraction time was 3 h. During the extraction process, carbon dioxide was flushed into the vessel. After the extraction was completed, the filtrate was filtered and collected; (5) The filtrates obtained in steps 2-4 were mixed and concentrated to 1200 ml to obtain an extract.

[0030] Example 7 Prescription of herbal combination for invigorating qi and nourishing blood: 200g of Rehmannia root, 100g of donkey-hide gelatin, 180g of Astragalus root, 100g of Codonopsis pilosula, 100g of red dates, 80g of Chinese yam, 80g of longan, 120g of Chinese angelica, 60g of ginseng, 50g of hawthorn, and 40g of licorice.

[0031] (1) Weigh the combined raw materials according to the prescribed amount, mix them evenly and crush them into a particle size of 5 μm, and then treat them with medium- and short-wave infrared equipment at 130°C for 25 min; (2) The raw material powder was mixed with water in a mass ratio of 1:10, placed in a reaction vessel, and the pressure was 190 MPa, the temperature was 100°C, and the extraction time was 3 h. During the extraction process, carbon dioxide was flushed into the vessel. After the extraction was completed, the filtrate was filtered and collected. (3) The filter residue obtained in step 2 was mixed with water in a mass ratio of 1:8, placed in a reaction vessel, and subjected to a pressure of 190 MPa, a temperature of 100°C, and a time of 1.5 h. Nitrogen was flushed into the vessel during the extraction process. After the extraction was completed, the filtrate was filtered and collected; (4) The filter residue obtained in step 3 was mixed with water in a mass ratio of 1:6, placed in a reaction vessel, and the pressure was 190 MPa, the temperature was 100°C, and the extraction time was 1 h. Nitrogen was flushed into the vessel during the extraction process. After the extraction was completed, the filtrate was filtered and collected; (5) The filtrates obtained in steps 2-4 were mixed and concentrated to 1200 ml to obtain an extract.

[0032] Comparative Example 1 Prescription of herbal combination for invigorating qi and nourishing blood: 200g of Rehmannia root, 100g of donkey-hide gelatin, 180g of Astragalus root, 100g of Codonopsis pilosula, 100g of red dates, 80g of Chinese yam, 80g of longan, 120g of Chinese angelica, 60g of ginseng, 50g of hawthorn, and 40g of licorice.

[0033] (1) Weigh the raw materials of each component according to the prescription, mix them evenly and then grind them into a particle size of 5 μm; (2) The raw material powder was mixed with water in a mass ratio of 1:10 and placed in a reaction vessel. The pressure was 0.05 MPa, the temperature was 60°C, and the extraction time was 3 h. During the extraction process, carbon dioxide was flushed into the vessel. After the extraction was completed, the filtrate was filtered and collected. (3) The filter residue obtained in step 2 was mixed with water in a mass ratio of 1:8, placed in a reaction vessel, and subjected to a pressure of 190 MPa, a temperature of 100°C, and a time of 1.5 h. Nitrogen was flushed into the vessel during the extraction process. After the extraction was completed, the filtrate was filtered and collected; (4) The filter residue obtained in step 3 was mixed with water in a mass ratio of 1:6, placed in a reaction vessel, and subjected to a pressure of 360 MPa, a temperature of 130°C, and a time of 1 h. During the extraction process, nitrogen was flushed into the vessel. After the extraction was completed, the filtrate was filtered and collected. (5) The filtrates obtained in steps 2-4 were mixed and concentrated to 1200 ml to obtain an extract.

[0034] Comparative Example 2 Prescription of herbal combination for invigorating qi and nourishing blood: 200g of Rehmannia root, 100g of donkey-hide gelatin, 180g of Astragalus root, 100g of Codonopsis pilosula, 100g of red dates, 80g of Chinese yam, 80g of longan, 120g of Chinese angelica, 60g of ginseng, 50g of hawthorn, and 40g of licorice.

[0035] (1) Weigh the combined raw materials according to the prescribed amount, mix them evenly and crush them into a particle size of 5 μm, and then treat them with medium- and short-wave infrared equipment at 130°C for 25 min; (2) Mix the raw material powder and water in a mass ratio of 1:10, place in a reaction vessel, pressure 0.1 MPa, temperature 60°C, time 3 h; after extraction, filter and collect the filtrate; (3) The filter residue obtained in step 2 was mixed with water in a mass ratio of 1:8, placed in a reaction vessel, and the pressure was 190 MPa, the temperature was 100 ° C, and the time was 1.5 h. After the extraction was completed, the filtrate was filtered and the filtrate was collected; (4) The filter residue obtained in step 3 was mixed with water in a mass ratio of 1:6, placed in a reaction vessel, and the pressure was 360 MPa, the temperature was 130 ° C, and the time was 1 h. After the extraction was completed, the filtrate was filtered and the filtrate was collected; (5) The filtrates obtained in steps 2-4 were mixed and concentrated to 1200 ml to obtain an extract.

[0036] Comparative Example 3 Prescription of herbal combination for invigorating qi and nourishing blood: 200g of Rehmannia root, 100g of donkey-hide gelatin, 180g of Astragalus root, 100g of Codonopsis pilosula, 100g of red dates, 80g of Chinese yam, 80g of longan, 120g of Chinese angelica, 60g of ginseng, 50g of hawthorn, and 40g of licorice.

[0037] (1) Weigh the combined raw materials according to the prescribed amount, mix them evenly and crush them into a particle size of 5 μm, and then treat them with medium- and short-wave infrared equipment at 130°C for 25 min; (2) The raw material powder was mixed with water in a mass ratio of 1:10 and placed in a reaction vessel. The pressure was 0.1 MPa, the temperature was 60°C, and the extraction time was 3 h. During the extraction process, carbon dioxide was flushed into the vessel. After the extraction was completed, the filtrate was filtered, collected, and concentrated to 1200 ml to obtain the extract.

[0038] Comparative Example 4 Prescription of herbal combination for invigorating qi and nourishing blood: 200g of Rehmannia root, 100g of donkey-hide gelatin, 180g of Astragalus root, 100g of Codonopsis pilosula, 100g of red dates, 80g of Chinese yam, 80g of longan, 120g of Chinese angelica, 60g of ginseng, 50g of hawthorn, and 40g of licorice.

[0039] (1) Weigh the combined raw materials according to the prescribed amount, mix them evenly and crush them into a particle size of 5 μm, and then treat them with medium- and short-wave infrared equipment at 130°C for 25 min; (2) The raw material powder was mixed with water in a mass ratio of 1:10 and placed in a reaction vessel. The pressure was 0.1 MPa, the temperature was 60°C, and the extraction time was 3 h. During the extraction process, carbon dioxide was flushed into the vessel. After the extraction was completed, the filtrate was filtered and collected. (3) The filter residue obtained in step 2 was mixed with water in a mass ratio of 1:8, placed in a reaction vessel, and subjected to a pressure of 190 MPa, a temperature of 100°C, and a time of 3 h. Nitrogen was flushed into the vessel during the extraction process. After the extraction was completed, the filtrate was filtered and collected; (5) The filtrates obtained in steps 2-3 were mixed and concentrated to 1200 ml to obtain an extract.

[0040] Comparative Example 5 Prescription of herbal combination for invigorating qi and nourishing blood: 200g of Rehmannia root, 100g of donkey-hide gelatin, 180g of Astragalus root, 100g of Codonopsis pilosula, 100g of red dates, 80g of Chinese yam, 80g of longan, 120g of Chinese angelica, 60g of ginseng, 50g of hawthorn, and 40g of licorice.

[0041] (1) Weigh the combined raw materials according to the prescribed amount, mix them evenly and crush them into a particle size of 5 μm, and then treat them with medium- and short-wave infrared equipment at 130°C for 25 min; (2) Mix the raw material powder with water in a mass ratio of 1:10, place it in a reaction vessel, and incubate at normal pressure, 60°C, for 3 hours. During the extraction process, flush the vessel with carbon dioxide. After the extraction is complete, filter and collect the filtrate. (3) The filter residue obtained in step 2 was mixed with water in a mass ratio of 1:8, placed in a reaction vessel, and incubated at normal pressure, 100°C, for 1.5 h. During the extraction process, nitrogen was flushed into the vessel. After the extraction was completed, the filtrate was filtered and collected. (4) The filter residue obtained in step 3 was mixed with water in a mass ratio of 1:6, placed in a reaction vessel, and incubated at normal pressure, 130°C, for 1 hour. During the extraction process, nitrogen was flushed into the vessel. After the extraction was completed, the filtrate was filtered and collected. (5) The filtrates obtained in steps 2-4 were mixed and concentrated to 1200 ml to obtain an extract.

[0042] Comparative Example 6 Prescription of herbal combination for invigorating qi and nourishing blood: 200g of Rehmannia root, 100g of donkey-hide gelatin, 180g of Astragalus root, 100g of Codonopsis pilosula, 100g of red dates, 80g of Chinese yam, 80g of longan, 120g of Chinese angelica, 60g of ginseng, 50g of hawthorn, and 40g of licorice.

[0043] (1) Weighing the combined raw materials of each component according to the prescribed amount, mixing, and grinding into a drug powder; mixing the drug powder, water, and cellulase in a weight ratio of 100:600:3, hydrolyzing at 50° C. for 5 hours, and inactivating the enzyme in a boiling water bath for 10 minutes to obtain a hydrolyzate; (2) mixing the hydrolyzate and Lactobacillus casei, wherein the amount of Lactobacillus casei added is 1% of the weight of the hydrolyzate; and fermenting at room temperature for 2 days to obtain a fermentation product; (3) adding ethanol to the fermented product until the ethanol concentration reaches 75%, and soaking for 1 hour to obtain a Chinese medicine soaking solution; (4) using a condensation reflux device to condense and reflux the Chinese medicine soaking liquid for 3 hours at a heating temperature of 70° C., filtering the condensed reflux liquid to obtain a Chinese medicine ethanol extract; (5) uniformly mixing the Chinese herbal medicine ethanol extract and an equal weight of isoprene glycol to obtain a mixed solution; (6) The mixed solution was concentrated to 1200 ml by rotary evaporation using a rotary evaporator and heated to 80°C to obtain an extract.

[0044] Verification Example 1. Extraction cream yield Take a certain amount of extract, place it in an evaporating dish that has been dried to constant weight, evaporate it to dryness in a water bath, dry it at 105℃ for 3 hours, cool it to constant weight, and then weigh it to calculate the paste rate.

[0045] Table 1 Extraction rate of paste It can be seen from the data in Table 1 that the extract yield of the extract obtained by the preparation method of the present invention reaches more than 37%, which greatly improves the yield of the extract.

[0046] , Determination of the contents of astragaloside IV, ferulic acid and ginsenosides in the extract HPLC was used to detect the contents of astragaloside IV, ferulic acid, and ginsenoside in each extract. The results are shown in Table 2.

[0047] Table 2 Contents of astragaloside IV, ferulic acid and ginsenosides in the extracts 3. Investigation of the improvement effect of reserpine on spleen qi deficiency model mice Ninety-six male, 7-week-old, SPF-grade Kunming mice were housed in a room maintained at (22±2)°C and 50±5% humidity, with free access to food and water. The mice were acclimated for 7 days prior to the experiment and randomly divided into 16 groups based on body weight: a model control group, a normal control group, and treatment groups (Examples 1-7 and Comparative Examples 1-7), with 6 mice in each group.

[0048] The experiment began with drug administration. Each drug-treated group was gavaged with the corresponding formula extract at a dose of 10 ml / kg / day, and the normal control group and model control group were given an equal amount of normal saline. After 2 weeks of administration, the model control group and each drug-treated group were intraperitoneally injected with reserpine (0.2 mg / kg) at an injection volume of 0.1 mL / 10 g body weight, and the normal control group was intraperitoneally injected with an equal volume of normal saline. One hour later, each drug-treated group continued to receive gavage (same dose as before), and the normal control group and model control group were gavaged with an equal volume of pure water for 2 consecutive weeks. The mice were weighed during the drug administration period, and the status of the mice in each group was closely observed. At the end of the drug administration, the symptoms of spleen deficiency in each group of mice were macroscopically scored.

[0049] Scoring criteria: (1) Eyes: full, 1 point; narrow, 2 points; closed, 3 points; (2) Stool: formed, 1 point; loose, 2 points; loose, 3 points; (3) Hair: smooth, 1 point; rough, 2 points; dull, 3 points; (4) Demeanor: lively, 1 point; lazy, 2 points; sleepy, 3 points.

[0050] The macroscopic scoring results of spleen deficiency syndrome of mice in each group are shown in Table 3.

[0051] Table 3 Symptom scores of mice in each group 4. Sensory and flavor evaluation Sensory and flavor evaluation of extract beverages is crucial for assessing their quality and consumer acceptance. The compositions obtained in the above examples and comparative examples were evaluated based on three key aspects: flavor, aroma, and texture. The scoring scale was 4:3:3. The scores were excellent (100 points), good (60 points), and poor (10 points). The results, shown in Table 4, show that different extraction processes produce different results for each component, which in turn affects the flavor, aroma, and texture of the extracts.

[0052] Table 4 Sensory and flavor evaluation results Group Evaluation result score Example 1 89.6 Example 2 86.7 Example 3 88.1 Example 4 77.3 Example 5 79.5 Example 6 80.1 Example 7 75.5 Comparative Example 1 58.9 Comparative Example 2 63.2 Comparative Example 3 61.7 Comparative Example 4 72.5 Comparative Example 5 69.1 Comparative Example 6 60.8

Claims

1. A combined extraction process and application of herbal plants for replenishing qi and blood, characterized in that: The steps include: 1) Grind the prescribed amount of the Qi-tonifying and blood-invigorating herbal combination raw materials and then process them using medium- and short-wave infrared equipment; 2) Mix the raw material powder with water, place it in a reaction vessel, adjust the pressure and temperature, and flush carbon dioxide into the vessel during the extraction process; after the extraction is completed, filter and collect the filtrate; 3) Mix the filter residue obtained in step 2 with water, place it in a reaction vessel, adjust the pressure and temperature, and flush nitrogen into the vessel during the extraction process; after the extraction is completed, filter and collect the filtrate; 4) Mix the filter residue obtained in step 3 with water, place it in a reaction vessel, adjust the pressure and temperature, and flush nitrogen into the vessel during the extraction process; after the extraction is completed, filter and collect the filtrate; 5) The filtrates obtained in steps 2) to 4) are mixed and concentrated to obtain an extract.

2. The method according to claim 1, characterized in that Step 1) The raw material is crushed to a particle size of 1-10 μm.

3. The method according to claim 1, characterized in that Step 1) The medium- and short-wave infrared equipment treatment conditions are 120-150° C. for 20-30 minutes.

4. The method according to claim 1, wherein In step 2), the raw material powder and water are mixed in a mass ratio of 1:8-10, at a pressure of 0.02-0.08 MPa, a temperature of 50-60° C., and a time of 2-3 hours.

5. The method according to claim 1, wherein Step 3) Mix the middle filter residue with water in a mass ratio of 1:6-8, at a pressure of 150-200 MPa, a temperature of 80-100°C, and a time of 1-2 hours.

6. The method according to claim 1, wherein Step 4) The filter residue is mixed with water in a mass ratio of 1:4-6, at a pressure of 300-400 MPa, a temperature of 120-140°C, and a time of 0.5-1 h.

7. The method according to claim 1, characterized in that The herbal combination for invigorating qi and nourishing blood comprises Rehmannia root, donkey-hide gelatin, astragalus root, Codonopsis pilosula, red dates, Chinese yam, longan, Chinese angelica, ginseng, hawthorn and liquorice.

8. The method according to claim 7, characterized in that The combination comprises 18-22 parts of Rehmannia root, 8-12 parts of donkey-hide gelatin, 16-20 parts of Astragalus root, 8-12 parts of Codonopsis pilosula, 8-12 parts of red dates, 6-10 parts of Chinese yam, 6-10 parts of longan, 10-14 parts of Chinese angelica, 4-8 parts of ginseng, 3-7 parts of hawthorn, and 2-6 parts of liquorice.

9. The method according to claim 8, characterized in that The combination comprises 20 parts of Rehmannia root, 10 parts of donkey-hide gelatin, 18 parts of Astragalus root, 10 parts of Codonopsis pilosula, 10 parts of red dates, 8 parts of Chinese yam, 8 parts of longan, 12 parts of Chinese angelica, 6 parts of ginseng, 5 parts of hawthorn, and 4 parts of liquorice.

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