Seaweed fish protein-containing microbial fertilizer and preparation method thereof

Abstract

The invention relates to the field of microbial fertilizers, and particularly discloses a microbial fertilizer containing seaweed fish protein and a preparation method of the microbial fertilizer. The bacterial fertilizer is prepared from the following raw materials in parts by weight: 40 to 50 parts of nitrogen-phosphorus-potassium fertilizer, 20 to 30 parts of humic acid, 1 to 4 parts of trace elements, 5 to 10 parts of seaweed zymolyte, 10 to 15 parts of fish protein zymolyte and 1 to 5 parts of bacterium-loaded fiber, the preparation method comprises the following steps: S1, weighing a nitrogen-phosphorus-potassium fertilizer, humic acid and trace elements, and uniformly mixing and stirring to obtain a primary mixture; and S2, adding the seaweed zymolyte, the fish protein zymolyte and the bacterium-loaded fiber into the primary mixture, and uniformly mixing and stirring to obtain a finished product, the cold-resistant microbial fertilizer has the advantages of good cold-resistant effect and high bacterial fertilizer absorption and utilization rate under low-temperature conditions, so that the crop yield is increased.

Description

Technical Field

[0001] The present application relates to the field of microbial fertilizers, and more specifically, to a microbial fertilizer containing seaweed fish protein and a preparation method thereof. Background Art

[0002] Microbial fertilizer is a new type of fertilizer that is a low-carbon, pure natural, non-toxic, harmless, and pollution-free organic microbial agent. It has the advantages of improving soil fertility, increasing the number and activity of beneficial microorganisms in the soil, improving soil activation, and preventing soil compaction. It can improve soil water retention capacity, promote crop growth, and increase crop yields.

[0003] Existing biological fertilizers have poor cold resistance. Wheat and other plants need to survive the winter. In recent years, extreme low temperature weather has often occurred in the central region and the southern region, which can easily affect the growth and yield of crops and plants. In addition, the low temperature environment can easily affect the absorption and utilization rate of fertilizers by crops and plants. Summary of the Invention

[0004] In order to prepare a new biological fertilizer that enables crops to have good cold resistance and high fertilizer absorption and utilization rate under low temperature conditions, thereby increasing crop yields, the present application provides a microbial fertilizer containing seaweed fish protein and a preparation method thereof.

[0005] In the first aspect, the present application provides a microbial fertilizer containing seaweed and fish protein, which adopts the following technical solution: a microbial fertilizer containing seaweed and fish protein, the fertilizer comprising the following raw materials in parts by weight: 40-50 parts of nitrogen, phosphorus and potassium fertilizers, 20-30 parts of humic acid, 1-4 parts of trace elements, 5-10 parts of seaweed hydrolysate, 10-15 parts of fish protein hydrolysate, and 1-5 parts of bacterial fiber.

[0006] By adopting the above technical scheme, seaweed enzymatic hydrolysate, fish protein enzymatic hydrolysate and bacterial-loaded fiber are combined, and seaweed polysaccharides can improve the stability of plant cell membranes and improve the cold resistance of plant cells. Combined with the high content of amino acids, peptides, minerals and trace elements contained in the fish protein enzymatic hydrolysate, the small molecule peptides in the fish protein decomposition can induce crops to synthesize unsaturated fatty acids, reduce the phase transition temperature of cell membrane lipids, prevent membrane lipid gelation and rupture at low temperatures, maintain membrane fluidity, and improve cold resistance; combined with the fiber loading capacity of the bacterial-loaded fiber, the contact area between the bacteria and the soil can be further increased, making it easier to adjust the soil environment; combined with the heat preservation and barrier effect of the fiber, combined with the natural active ingredients in the seaweed enzymatic hydrolysate and fish protein enzymatic hydrolysate, the activity of microorganisms in the soil can be improved, so that the beneficial bacteria in the soil still have a certain survival rate and reproduction capacity under low temperature conditions, the bacterial fertilizer can continuously provide nutrients for crops, and the nutrients in the enzymatic hydrolysate are gradually absorbed by the plants, which can enhance the cold resistance of crops, thereby ensuring the effective utilization rate of the bacterial fertilizer in the soil at low temperatures.

[0007] The combination of seaweed hydrolysate, fish protein hydrolysate, bacterial fiber, nitrogen, phosphorus and potassium fertilizers, humic acid and trace elements can promote plant growth and increase crop yields.

[0008] Preferably, the seaweed hydrolysate is prepared by crushing Ascophyllum nodosum, adding water, performing low-temperature enzymolysis, inactivating enzymes, filtering, and concentrating to obtain a concentrate, and adding a loading molecular sieve to the concentrate at a mass ratio of 10:0.5-1, followed by dispersion, drying, and breaking up.

[0009] By adopting the above technical solution, the specific surface area of ​​the Ascophyllum nodosum is increased after being crushed, and the contact area with the enzyme is increased. During the low-temperature enzymatic hydrolysis process, the natural active ingredients in the Ascophyllum nodosum are retained, which helps to improve the low-temperature adaptability of microorganisms in the microbial fertilizer. It can still maintain a certain activity under low-temperature conditions, thereby improving the absorption and utilization rate of the microbial fertilizer under low-temperature conditions. In addition, the crushing and low-temperature enzymatic hydrolysis processes help to release the nutrients in the Ascophyllum nodosum, making it easier to be absorbed by microorganisms and plant roots. These nutrients can also remain stable in a low-temperature environment, which helps to continuously supply the microbial fertilizer, so that crops can still absorb the nutrients in the microbial fertilizer under low-temperature conditions, promote the low-temperature growth of crops, and increase crop yields. The concentration and purity of the microbial fertilizer are increased by concentration, thereby further improving the stability and effectiveness of the microbial fertilizer under low-temperature conditions.

[0010] The concentrated liquid is combined with the loading molecular sieve, and the absorption of the molecular sieve is utilized to facilitate the adsorption of the concentrated liquid. The loading effect of the molecular sieve is utilized to protect the effective ingredients in the concentrated liquid, which not only prolongs the action time of the effective ingredients, but also the barrier effect of the pores can improve the cold resistance of the concentrated liquid and protect the activity of the effective ingredients under low temperature conditions, thereby ensuring the stability and activity of the bacterial fertilizer in a low temperature environment, promoting crop growth and increasing crop yields.

[0011] Preferably, the loaded molecular sieve is prepared from molecular sieve, polyvinyl alcohol-1799 solution and zinc polyaspartate in a mass ratio of 1:0.1-0.25:0.1-0.15.

[0012] By adopting the above technical solution, the molecular sieve, polyvinyl alcohol-1799 solution and zinc polyaspartate are combined, and the zinc polyaspartate on the surface of the loaded molecular sieve can complex with alginate in the seaweed hydrolysate to form a stable complex, thereby ensuring the solubility and stability of alginate in a low-temperature environment, and ensuring that alginate under low-temperature conditions can act on plants, promote crop growth and increase crop yields.

[0013] Molecular sieve has a certain adsorption capacity and can fix and slowly release the nutrients in the bacterial fertilizer. In a low temperature environment, the slow-release effect helps to maintain the activity of the bacterial fertilizer and continuously provide nutrients to crops, thereby improving the absorption and utilization rate of nutrients. Polyvinyl alcohol-1799 contains hydroxyl groups but is not easily soluble in water. It can have a certain moisturizing effect, preventing it from crystallizing or freezing due to low temperatures, thereby improving the low-temperature resistance of the bacterial fertilizer. Combined with polyvinyl alcohol-1799 solution, it improves the viscosity and dispersibility of the bacterial fertilizer, making it easier to mix with the soil, thereby improving the absorption and utilization rate of the bacterial fertilizer.

[0014] Preferably, the enzymes for low-temperature enzymatic hydrolysis are composed of cellulase and pectinase, the enzymatic hydrolysis temperature is 42-45° C., and the enzymatic hydrolysis time is 3-5 h.

[0015] By adopting the above technical solution, cellulase and pectinase respectively decompose the cellulose and pectin in the Ascophyllum nodosum, thereby improving the enzymatic hydrolysis efficiency and product yield. In addition, low-temperature enzymatic hydrolysis can avoid the influence of high temperature on the active substances in the Ascophyllum nodosum, ensuring that the enzymatic hydrolyzate has a high nutrient content.

[0016] Preferably, the fish protein hydrolysate is prepared by drying and crushing the fish product, adding water, then enzymolyzing, inactivating the enzyme, and filtering to obtain an enzymatic solution, adding calcium-based bentonite to the enzymatic solution at a mass ratio of 10:1-1.5, and performing dispersion treatment, drying, and breaking up.

[0017] By adopting the above technical solution, after the fish products are enzymatically hydrolyzed, the enzymatic hydrolysate contains rich amino acids and small peptides, which can provide nutrition for the microorganisms in the microbial fertilizer and the microorganisms in the soil. Calcium-based bentonite is added to the enzymatic hydrolysate. The calcium-based bentonite has an adsorption effect but is not prone to excessive expansion. It can effectively block the moisture and nutrients in the fish protein hydrolysate. The layered barrier properties of the calcium-based bentonite are utilized to improve the low-temperature resistance of the fish protein hydrolysate. Combined with the calcium element contained, it further promotes the absorption and utilization of nutrients in the microbial fertilizer by plants under low-temperature environments, promotes crop growth, and increases crop yields.

[0018] Preferably, the calcium-based bentonite is prepared from calcium-based bentonite particles and a high DE value maltodextrin solution in a mass ratio of 1:0.1-0.25.

[0019] By adopting the above technical solution, calcium-based bentonite particles and high-DE maltodextrin solution are combined. The high-DE maltodextrin solution has good water solubility and stability, which can dissolve to form a protective film, effectively prevent the excessive growth of ice crystals, reduce the damage of low temperature to the microbial fertilizer and microbial cells in the soil, ensure the activity of the microbial fertilizer, and ensure the growth of microorganisms in the soil. By regulating the osmotic pressure inside the microbial fertilizer, it helps microbial cells maintain normal metabolic activities in a low-temperature environment and prevents cell rupture or death caused by imbalance of osmotic pressure inside and outside the cells; at the same time, the high-DE maltodextrin solution can release energy under low-temperature conditions, provide necessary heat support for the microorganisms in the microbial fertilizer, help them resist the adverse effects of the low-temperature environment, and ensure the low-temperature activity and absorption utilization rate of the microbial fertilizer.

[0020] Preferably, the bacterial-loaded fiber is prepared from corn fiber, glutathione solution and composite bacteria in a mass ratio of 1:0.1-0.2:0.2-0.4, and the composite bacteria is composed of Bacillus subtilis, Bacillus megaterium and Lactobacillus plantarum in a mass ratio of 1:0.5-1:0.5-1.

[0021] By adopting the above technical solution, Bacillus subtilis, Bacillus megaterium and Lactobacillus plantarum are stably attached to the surface of corn fiber. The corn fiber can not only provide a good living environment for the strains, but also can be naturally degraded to provide nutrients for the bacteria, promote the growth and reproduction of the bacteria, and cooperate with Bacillus subtilis, Bacillus megaterium and Lactobacillus plantarum to produce growth hormones that promote the development of plant roots, making it easier for the roots to absorb and utilize nutrients. In addition, the bacteria can decompose organic matter and continuously release nutrients such as nitrogen, phosphorus and potassium, thereby promoting crop growth and increasing crop yields. At the same time, it can enable crops to have better resistance in low temperature environments and ensure the low-temperature growth rate of crops.

[0022] Preferably, the nitrogen, phosphorus and potassium fertilizers are composed of ammonium nitrate, urea and potassium dihydrogen phosphate in a mass ratio of 2:1-1.2:1-1.5.

[0023] By adopting the above technical solutions, nutrient elements can be provided to promote crop growth and increase crop yield.

[0024] Preferably, the trace elements are one or more of zinc sulfate, calcium chloride and magnesium chloride.

[0025] By adopting the above technical solution, zinc, calcium, magnesium and other substances are provided to promote crop growth and increase crop yield.

[0026] In a second aspect, the present application provides a method for preparing a microbial fertilizer containing seaweed and fish protein, which adopts the following technical solution: A method for preparing a microbial fertilizer containing seaweed and fish protein comprises the following steps: S1. Weigh nitrogen, phosphorus and potassium fertilizers, humic acid and trace elements and mix them evenly to obtain a primary mixture; S2. Add seaweed enzymatic hydrolysate, fish protein enzymatic hydrolysate and bacterial-loaded fiber to the primary mixed material, mix and stir evenly to obtain a finished product.

[0027] By adopting the above technical solution, biological fertilizer can make crops have good cold resistance and high absorption and utilization rate of fertilizer under low temperature conditions, thereby increasing crop yields.

[0028] In summary, this application has the following beneficial effects: 1. The combination of seaweed hydrolysate, fish protein hydrolysate, bacterial fiber, nitrogen, phosphorus and potassium fertilizers, humic acid and trace elements can promote plant growth and increase crop yields. In addition, seaweed hydrolysate and fish protein hydrolysate can improve the cold resistance of the bacterial fertilizer itself while improving the cold resistance of crops, so that crops can still have a good absorption and utilization rate of the bacterial fertilizer in a low temperature environment, promote crop growth and increase crop yields.

[0029] 2. The combination of seaweed hydrolysate, fish protein hydrolysate and bacterial-loaded fiber can improve the absorption and utilization rate of bacterial fertilizer by plants by utilizing the rapid absorption and penetration effect of small molecules such as peptides, amino acids and nutrients in the hydrolysate. The fiber loading capacity of the bacterial-loaded fiber can further increase the contact area between the bacteria and the soil, making it easier to adjust the soil environment. The fixation and barrier effect of the fiber can further ensure that seaweed hydrolysate, fish protein hydrolysate, bacteria and other substances are stably dispersed in the soil and are not easily washed away and migrated, further ensuring the effective utilization rate of bacterial fertilizer in the soil.

[0030] 3. The molecular sieve partially absorbs the concentrated liquid, and the remaining concentrated liquid can quickly release nutrients to supply crop needs after drying in the bacterial fertilizer, and the adsorbed ones can prolong the release time. Similarly, calcium-based bentonite can absorb part of the enzymatic hydrolysate. The unabsorbed enzymatic hydrolysate can be quickly released for crop absorption and utilization after drying and added to the soil in the bacterial fertilizer to ensure crop growth. The adsorbed ones can prolong the action time. At around -5℃ in autumn and winter, it can ensure the nutritional supply of the bacterial fertilizer to the crops, and can also ensure the growth of the crops and the yield of the crops. DETAILED DESCRIPTION

[0031] The present application is further described in detail below with reference to the embodiments.

[0032] Preparation example of seaweed hydrolysate Among the following raw materials, zinc polyaspartate was purchased from Jining Yuanlian Chemical Technology Co., Ltd.; cellulase and pectinase were purchased from Jiangsu Caiwei Biotechnology Co., Ltd.; and other raw materials were commercially available.

[0033] Preparation Example 1: Seaweed hydrolysate was prepared by the following method: 0.18 kg of polyvinyl alcohol-1799 solution was evenly sprayed on the surface of the molecular sieve, where the mass fraction of the polyvinyl alcohol-1799 solution was 1%, the solvent was water at a temperature of 95°C, and the average particle size of the molecular sieve was 25 μm. Then, 0.12 kg of zinc polyaspartate was added, and the average particle size of the zinc polyaspartate was 5 μm. After the addition of zinc polyaspartate, the mixture was immediately air-dried and cooled at 35°C, and then allowed to stand at room temperature for 1 hour to prevent the dispersed molecular sieves from sticking to each other and agglomerating, thereby obtaining a loaded molecular sieve. After drying, the Ascophyllum nodosum was crushed through an 80-mesh sieve to obtain a powder, water was added to the powder and stirred evenly, with a liquid-to-solid ratio of 30:1 (mL / g), and then cellulase and pectinase were added, with the cellulase concentration being 180 IU / mL and the pectinase concentration being 200 IU / mL. The enzymatic hydrolysis temperature was 44°C, the enzymatic hydrolysis time was 4 hours, and then the temperature was raised to 90°C to inactivate the enzymes for 15 minutes. The filtrate was then filtered and concentrated to a solid content of 25% to obtain a concentrate. 0.8 kg of loading molecular sieve was added to 10 kg of concentrated solution, and the mixture was stirred and dispersed at a rotation speed of 1000 r / min for 5 minutes, and then freeze-dried and crushed to obtain seaweed hydrolysate, which was passed through a 400-mesh sieve.

[0034] Preparation Example 2: This preparation example differs from Preparation Example 1 in that: 0.1 kg of polyvinyl alcohol-1799 solution was evenly sprayed on the surface of the molecular sieve, and then 0.1 kg of zinc polyaspartate was added. After adding zinc polyaspartate, it was immediately air-dried and cooled at 35 ° C, and then allowed to stand at room temperature for 1 hour to disperse the molecular sieves without sticking to each other and agglomerating to obtain the loaded molecular sieve; After drying, the Ascophyllum nodosum was crushed through an 80-mesh sieve to obtain a powder, water was added to the powder and stirred evenly, with a liquid-to-solid ratio of 30:1 (mL / g), and then cellulase and pectinase were added, with the cellulase concentration being 180 IU / mL and the pectinase concentration being 200 IU / mL. The enzymatic hydrolysis temperature was 42°C, the enzymatic hydrolysis time was 5 hours, and then the temperature was raised to 90°C to inactivate the enzymes for 15 minutes. The filtrate was then filtered and concentrated to a solid content of 25% to obtain a concentrate. 0.5 kg of loading molecular sieve was added to 10 kg of concentrated solution, and the mixture was stirred and dispersed at a rotation speed of 1000 r / min for 5 minutes, and then freeze-dried and crushed to obtain seaweed hydrolysate, which was passed through a 400-mesh sieve.

[0035] Preparation Example 3: This preparation example differs from Preparation Example 1 in that: 0.25 kg of polyvinyl alcohol-1799 solution was evenly sprayed on the surface of the molecular sieve, and then 0.15 kg of zinc polyaspartate was added. After adding zinc polyaspartate, it was immediately air-dried and cooled at 35 ° C, and then allowed to stand at room temperature for 1 hour to disperse the molecular sieves without sticking to each other and agglomerating to obtain the loaded molecular sieve; After drying, the Ascophyllum nodosum was crushed through an 80-mesh sieve to obtain a powder, water was added to the powder and stirred evenly, with a liquid-to-solid ratio of 30:1 (mL / g), and then cellulase and pectinase were added, with the cellulase concentration being 180 IU / mL and the pectinase concentration being 200 IU / mL. The enzymatic hydrolysis temperature was 45°C, the enzymatic hydrolysis time was 3 hours, and then the temperature was raised to 90°C to inactivate the enzymes for 15 minutes. The filtrate was then filtered and concentrated to a solid content of 25% to obtain a concentrate. 1 kg of molecular sieve loading material was added to 10 kg of concentrated solution, and the mixture was stirred and dispersed at a rotation speed of 1000 r / min for 5 minutes, and then freeze-dried and crushed to obtain seaweed hydrolysate, which was passed through a 400-mesh sieve.

[0036] Preparation example of fish protein hydrolysate The following raw materials are all commercially available.

[0037] Preparation Example 4: Fish protein hydrolysate was prepared by the following method: 0.2 kg of high DE value maltodextrin solution is evenly sprayed on the surface of 1 kg of calcium-based bentonite particles, wherein the average particle size of the calcium-based bentonite particles is 20 μm, the mass fraction of the high DE value maltodextrin solution is 1%, the solvent is water, and the DE value is 18%, and then the mixture is dried and dispersed until the calcium-based bentonite particles do not stick to each other and agglomerate, thereby obtaining calcium-based bentonite; Fish products are prepared by drying fish bones, fish skin, fish meat, etc. and then grinding them through an 80-mesh sieve to obtain powder. Water is added to the powder and stirred evenly. The liquid-to-solid ratio is 10:1 (mL / g). Papain is then added. The enzymatic activity of papain is 10,000 IU / g. 55g of papain is added per kilogram of powder. The enzymatic hydrolysis temperature is 40°C and the enzymatic hydrolysis time is 5h. The temperature is then raised to 90°C to inactivate the enzyme for 15min. The filtrate is then filtered and concentrated to a solid content of 25% to obtain an enzymatic hydrolyzate. 1.2 kg of calcium bentonite was added to 10 kg of enzymatic hydrolysate, and the mixture was stirred and dispersed at a rotation speed of 1000 r / min for 5 minutes, and then freeze-dried and crushed to obtain fish protein hydrolysate, which was passed through a 400-mesh sieve.

[0038] Preparation Example 5: This preparation example differs from Preparation Example 4 in that: 0.1 kg of high DE value maltodextrin solution is evenly sprayed on the surface of 1 kg of calcium-based bentonite particles, and then dried and dispersed until the calcium-based bentonite particles do not stick to each other and agglomerate to obtain calcium-based bentonite; Fish products are prepared by drying fish bones, fish skin, fish meat, etc. and then grinding them through an 80-mesh sieve to obtain powder. Water is added to the powder and stirred evenly. The liquid-to-solid ratio is 10:1 (mL / g). Papain is then added. The enzymatic activity of papain is 10,000 IU / g. 55g of papain is added per kilogram of powder. The enzymatic hydrolysis temperature is 40°C and the enzymatic hydrolysis time is 5h. The temperature is then raised to 90°C to inactivate the enzyme for 15min. The filtrate is then filtered and concentrated to a solid content of 25% to obtain an enzymatic hydrolyzate. 1 kg of calcium bentonite was added to 10 kg of enzymatic hydrolysate, and the mixture was stirred and dispersed at a rotation speed of 1000 r / min for 5 minutes, and then freeze-dried and crushed to obtain fish protein hydrolysate, which was passed through a 400-mesh sieve.

[0039] Preparation Example 6: This preparation example differs from Preparation Example 4 in that: 0.25 kg of high DE value maltodextrin solution is evenly sprayed on the surface of 1 kg of calcium-based bentonite particles, and then dried and dispersed until the calcium-based bentonite particles do not stick to each other and agglomerate to obtain calcium-based bentonite; Fish products are prepared by drying fish bones, fish skin, fish meat, etc. and then grinding them through an 80-mesh sieve to obtain powder. Water is added to the powder and stirred evenly. The liquid-to-solid ratio is 10:1 (mL / g). Papain is then added. The enzymatic activity of papain is 10,000 IU / g. 55g of papain is added per kilogram of powder. The enzymatic hydrolysis temperature is 40°C and the enzymatic hydrolysis time is 5h. The temperature is then raised to 90°C to inactivate the enzyme for 15min. The filtrate is then filtered and concentrated to a solid content of 25% to obtain an enzymatic hydrolyzate. 1.5 kg of calcium bentonite was added to 10 kg of enzymatic hydrolysate, and the mixture was stirred and dispersed at a rotation speed of 1000 r / min for 5 minutes, and then freeze-dried and crushed to obtain fish protein hydrolysate, which was passed through a 400-mesh sieve.

[0040] Preparation example of bacterial fiber Among the following raw materials, Bacillus subtilis, Bacillus megaterium, and Lactobacillus plantarum were purchased from Jiangsu Caiwei Biotechnology Co., Ltd.; other raw materials were commercially available.

[0041] Preparation Example 7: Bacteria-loaded fiber was prepared by the following method: 0.15 kg of glutathione solution was evenly sprayed on the surface of 1 kg of corn fiber. The average length of the corn fiber was 2 mm. The mass fraction of the glutathione solution was 2%. The solvent was water. Then, 0.3 kg of composite bacteria was added. The composite bacteria was prepared by mixing bacterial powders of Bacillus subtilis, Bacillus megaterium, and Lactobacillus plantarum in a mass ratio of 1:0.7:0.8. The composite bacteria was added at a rate of 60 g / min. During the addition process, the corn fiber was continuously stirred at a speed of 120 r / min. After being evenly mixed, the mixture was dried and dispersed until the corn fibers did not stick to each other and agglomerate, thereby obtaining bacteria-loaded fiber.

[0042] Preparation Example 8: This preparation example differs from Preparation Example 7 in that: 0.1 kg of glutathione solution was evenly sprayed on the surface of 1 kg of corn fiber. The average length of the corn fiber was 2 mm. Then, 0.2 kg of composite bacteria was added. The composite bacteria was prepared by mixing the powders of Bacillus subtilis, Bacillus megaterium and Lactobacillus plantarum in a mass ratio of 1:0.5:0.5. The addition rate of the composite bacteria was 60 g / min. During the addition process, the corn fiber was continuously stirred at a speed of 120 r / min. After being evenly mixed, it was dried and dispersed until the corn fibers did not stick to each other and agglomerate to obtain bacteria-loaded fiber.

[0043] Preparation Example 9: This preparation example differs from Preparation Example 7 in that: 0.2 kg of glutathione solution was evenly sprayed on the surface of 1 kg of corn fiber. The average length of the corn fiber was 2 mm. Then, 0.4 kg of composite bacteria was added. The composite bacteria was prepared by mixing the powders of Bacillus subtilis, Bacillus megaterium and Lactobacillus plantarum in a mass ratio of 1:1:1. The addition rate of the composite bacteria was 60 g / min. During the addition process, the corn fiber was continuously stirred at a speed of 120 r / min. After being evenly mixed, it was dried and dispersed until the corn fibers did not stick to each other and agglomerate to obtain the bacteria-loaded fiber. Example

[0044] The following raw materials are all commercially available.

[0045] Example 1: A microbial fertilizer containing seaweed and fish protein: 45 kg of nitrogen, phosphorus and potassium fertilizers, 25 kg of humic acid, 2.5 kg of trace elements, 8 kg of seaweed hydrolysate, 12 kg of fish protein hydrolysate, and 3 kg of bacterial cellulosic material; the nitrogen, phosphorus and potassium fertilizers are composed of ammonium nitrate, urea and potassium dihydrogen phosphate in a mass ratio of 2:1.2:1.3, the trace elements are composed of zinc sulfate, calcium chloride and magnesium chloride in a mass ratio of 1:1:0.5, the seaweed hydrolysate is the seaweed hydrolysate prepared in Preparation Example 1, the fish protein hydrolysate is the fish protein hydrolysate prepared in Preparation Example 4, and the bacterial cellulosic material is the bacterial cellulosic material prepared in Preparation Example 7; The preparation method is as follows: S1. Weigh nitrogen, phosphorus and potassium fertilizers, humic acid and trace elements and mix them evenly to obtain a primary mixture; S2. Add seaweed enzymatic hydrolysate, fish protein enzymatic hydrolysate and bacterial-loaded fiber to the primary mixed material, mix and stir evenly to obtain a finished product.

[0046] Example 2: This example differs from Example 1 in that: 40 kg of nitrogen, phosphorus and potassium fertilizers, 20 kg of humic acid, 1 kg of trace elements, 5 kg of seaweed hydrolysate, 10 kg of fish protein hydrolysate, and 1 kg of bacterial cellulosic material; the nitrogen, phosphorus and potassium fertilizers are composed of ammonium nitrate, urea and potassium dihydrogen phosphate in a mass ratio of 2:1:1, the trace elements are composed of zinc sulfate, calcium chloride and magnesium chloride in a mass ratio of 1:1:1, the seaweed hydrolysate adopts the seaweed hydrolysate prepared in Preparation Example 2, the fish protein hydrolysate adopts the fish protein hydrolysate prepared in Preparation Example 5, and the bacterial cellulosic material adopts the bacterial cellulosic material prepared in Preparation Example 8.

[0047] Example 3: This example differs from Example 1 in that: 50 kg of nitrogen, phosphorus and potassium fertilizers, 30 kg of humic acid, 4 kg of trace elements, 10 kg of seaweed hydrolysate, 15 kg of fish protein hydrolysate, and 5 kg of bacterial cellulosic material; the nitrogen, phosphorus and potassium fertilizers are composed of ammonium nitrate, urea and potassium dihydrogen phosphate in a mass ratio of 2:1.2:1.5, the trace elements are composed of zinc sulfate, calcium chloride and magnesium chloride in a mass ratio of 1:1:1, the seaweed hydrolysate adopts the seaweed hydrolysate prepared in Preparation Example 3, the fish protein hydrolysate adopts the fish protein hydrolysate prepared in Preparation Example 6, and the bacterial cellulosic material adopts the bacterial cellulosic material prepared in Preparation Example 9.

[0048] Example 4: This example differs from Example 1 in that: During the preparation of seaweed hydrolysate, no molecular sieve was added as loading material.

[0049] Example 5: This example differs from Example 1 in that: During the preparation of seaweed hydrolysate, no polyvinyl alcohol-1799 solution and zinc polyaspartate were added to the loaded molecular sieve.

[0050] Example 6: This example differs from Example 1 in that: No calcium bentonite was added during the preparation of fish protein hydrolysate.

[0051] Example 7: This example differs from Example 1 in that: During the preparation of fish protein hydrolysate, calcium bentonite was not added with high DE value maltodextrin solution.

[0052] Example 8: This example differs from Example 1 in that: No corn fiber and glutathione solution were added during the preparation of the bacterial-loaded fiber.

[0053] Comparative Example Comparative Example 1: The difference between this comparative example and Example 1 is that: No seaweed hydrolysate or fish protein hydrolysate is added to the raw materials.

[0054] Performance testing 1. Crop yield detection Bacterial fertilizers were prepared by the methods of Examples 1-8 and Comparative Example 1, with each acre of land receiving the corresponding bacterial fertilizer of the embodiment or comparative example. The bacterial fertilizer was applied to the soil surface and plowed into the soil to a depth of about 15-20 cm. 60 kg of the bacterial fertilizer was added to each acre of land as a base fertilizer for the bacterial fertilizer. Wheat was planted. When the wheat seedlings were about 15-18 cm in height and had about 5-7 leaves, a low temperature was simulated at -5°C for 24 hours. The frostbite area of ​​the leaves was recorded, with level 0 being no frostbite, level 1 being a frostbite area of ​​0 < X ​​≤ 5%, level 2 being a frostbite area of ​​5% < X ≤ 15%, level 3 being a frostbite area of ​​15% < X ≤ 40%, and level 4 being a frostbite area of ​​40% < X. After 7 days of simulating the low temperature at -5°C, the survival rate of the plants was recorded. Finally, the wheat yield was recorded during the wheat harvest season.

[0055] Table 1 Performance test table project Frostbite area / grade Survival rate / % Yield / kg / mu Example 1 0 85 765 Example 2 0 82 759 Example 3 0 86 767 Example 4 2 77 742 Example 5 1 80 750 Example 6 2 75 737 Example 7 1 79 748 Example 8 2 76 740 Comparative Example 1 3 55 610 It can be seen from Examples 1-3 and Table 1 that the bacterial fertilizer prepared in the present application has good cold resistance and can provide nutrients for crops to absorb under low temperature conditions. After the crops absorb the bacterial fertilizer, their own cold resistance effect increases, thereby increasing wheat yield.

[0056] Combining Example 1 and Examples 4-8 with Table 1, it can be seen that in the preparation process of the seaweed hydrolysate in Example 4, no loading molecular sieve was added. Compared with Example 1, the frostbite area in Example 4 was larger than that in Example 1, the survival rate was lower than that in Example 1, and the yield was lower than that in Example 1; this shows that the barrier effect of the molecular sieve can further improve the cold resistance, so that the concentrate has better activity under low temperature conditions, ensure the stability and activity of the bacterial fertilizer in a low temperature environment, promote crop growth, and increase crop yield.

[0057] In the preparation process of seaweed hydrolysate in Example 5, no polyvinyl alcohol-1799 solution and zinc polyaspartate were added to the loaded molecular sieve. Compared with Example 1, the frostbite area in Example 5 was larger than that in Example 1, the survival rate was lower than that in Example 1, and the yield was lower than that in Example 1. This shows that the polyvinyl alcohol-1799 solution and zinc polyaspartate are matched. Polyvinyl alcohol-1799 contains hydroxyl groups but is not easily soluble in water, and can have a certain moisturizing effect, preventing it from crystallizing or freezing due to low temperature, thereby improving the low temperature resistance of the bacterial fertilizer. The zinc element in zinc polyaspartate further stimulates the stress resistance gene in the plant body, thereby ensuring the plant's ability to absorb and utilize alginic acid in a low temperature environment, promoting crop growth, and increasing crop yield.

[0058] No calcium-based bentonite was added during the preparation of the fish protein hydrolysate in Example 6. Compared with Example 1, the frostbite area in Example 6 was larger than that in Example 1, the survival rate was lower than that in Example 1, and the yield was lower than that in Example 1. This indicates that the layered barrier properties of calcium-based bentonite are utilized to improve the low-temperature resistance of the fish protein hydrolysate, and the calcium element contained therein further promotes the absorption and utilization of nutrients in the microbial fertilizer by plants under low-temperature environments, thereby promoting crop growth and increasing crop yield.

[0059] In the preparation process of fish protein hydrolysate in Example 7, calcium bentonite was not added with high DE value maltodextrin solution. Compared with Example 1, the frostbite area of ​​Example 7 was larger than that of Example 1, the survival rate was lower than that of Example 1, and the yield was lower than that of Example 1; this indicates that the high DE value maltodextrin solution has good water solubility and stability, can dissolve at low temperatures to form a protective film, effectively prevent the excessive growth of ice crystals, reduce the damage of low temperature to the microbial fertilizer and microbial cells in the soil, and ensure the activity of the microbial fertilizer. The high DE value maltodextrin solution can release energy under low temperature conditions, provide necessary heat support for the microorganisms in the microbial fertilizer, help them resist the adverse effects of the low temperature environment, ensure the low temperature activity and absorption utilization rate of the microbial fertilizer, thereby ensuring the growth of crops.

[0060] In Example 8, no corn fiber and glutathione solution were added during the preparation of the bacterial fiber. Compared with Example 1, the frostbite area of ​​Example 8 was larger than that of Example 1, the survival rate was lower than that of Example 1, and the yield was lower than that of Example 1. This indicates that the porous structure of corn fiber has a temperature-control effect, and glutathione can promote Bacillus subtilis to secrete antifreeze proteins and induce crops to synthesize regulatory substances, thereby improving the cold resistance of crops, ensuring crop growth, and ensuring crop yield.

[0061] Combining Example 1 and Comparative Example 1 with Table 1, it can be seen that no seaweed enzymatic hydrolysate and fish protein enzymatic hydrolysate were added to the raw materials of Comparative Example 1. Compared with Example 1, the frostbite area of ​​Comparative Example 1 was larger than that of Example 1, the survival rate was lower than that of Example 1, and the yield was lower than that of Example 1; this indicates that the combination of seaweed enzymatic hydrolysate and fish protein enzymatic hydrolysate can further improve the cold resistance of crops.

[0062] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make non-creative modifications to the present embodiment as needed, but as long as they are within the scope of the claims of the present application, they are protected by the patent law.

Claims

1. A microbial fertilizer containing seaweed and fish protein, characterized in that: The bacterial fertilizer comprises the following raw materials in parts by weight: 40-50 parts of nitrogen, phosphorus and potassium fertilizers, 20-30 parts of humic acid, 1-4 parts of trace elements, 5-10 parts of seaweed hydrolysate, 10-15 parts of fish protein hydrolysate, and 1-5 parts of bacterial fiber.

2. The microbial fertilizer containing seaweed and fish protein according to claim 1, characterized in that: The seaweed hydrolysate is prepared by crushing Ascophyllum nodosum, adding water, performing low-temperature enzymolysis, inactivating enzymes, filtering, and concentrating to obtain a concentrated solution, adding a loading molecular sieve to the concentrated solution at a mass ratio of 10:0.5-1, and then dispersing, drying, and breaking up the solution.

3. The microbial fertilizer containing seaweed fish protein according to claim 2, characterized in that: The loaded molecular sieve is prepared from molecular sieve, polyvinyl alcohol-1799 solution and zinc polyaspartate in a mass ratio of 1:0.1-0.25:0.1-0.

15.

4. The microbial fertilizer containing seaweed and fish protein according to claim 2, characterized in that: The enzymes for low-temperature enzymatic hydrolysis are composed of cellulase and pectinase, the enzymatic hydrolysis temperature is 42-45° C., and the enzymatic hydrolysis time is 3-5 hours.

5. The microbial fertilizer containing seaweed and fish protein according to claim 1, characterized in that: The fish protein hydrolysate is prepared by drying and crushing fish products, adding water, then enzymolyzing, inactivating the enzyme, and filtering to obtain an enzymatic solution. Calcium-based bentonite is added to the enzymatic solution at a mass ratio of 10:1-1.5, and the product is dispersed, dried, and broken up.

6. The microbial fertilizer containing seaweed and fish protein according to claim 5, characterized in that: The calcium-based bentonite is prepared from calcium-based bentonite particles and a high-DE value maltodextrin solution in a mass ratio of 1:0.1-0.

25.

7. The microbial fertilizer containing seaweed and fish protein according to claim 1, characterized in that: The bacteria-loaded fiber is prepared from corn fiber, glutathione solution and composite bacteria in a mass ratio of 1:0.1-0.2:0.2-0.4, and the composite bacteria is composed of Bacillus subtilis, Bacillus megaterium and Lactobacillus plantarum in a mass ratio of 1:0.5-1:0.5-1.

8. The microbial fertilizer containing seaweed and fish protein according to claim 1, characterized in that: The nitrogen, phosphorus and potassium fertilizer consists of ammonium nitrate, urea and potassium dihydrogen phosphate in a mass ratio of 2:1-1.2:1-1.

5.

9. The microbial fertilizer containing seaweed and fish protein according to claim 1, characterized in that: The trace elements are selected from one or more of zinc sulfate, calcium chloride and magnesium chloride.

10. The method for preparing a microbial fertilizer containing seaweed and fish protein according to any one of claims 1 to 9, characterized in that: The following steps are involved: S1. Weigh nitrogen, phosphorus and potassium fertilizers, humic acid and trace elements and mix them evenly to obtain a primary mixture; S2. Add seaweed enzymatic hydrolysate, fish protein enzymatic hydrolysate and bacterial-loaded fiber into the primary mixed material, mix and stir evenly to obtain a finished product.