Microorganism-containing potassium fulvate water-soluble fertilizer and preparation method thereof

Abstract

The invention relates to the technical field of fertilizers, in particular to a water-soluble fertilizer containing microbial potassium fulvate and a preparation method of the water-soluble fertilizer. The method comprises the following steps: providing a first molasses liquid and a second molasses liquid; adding a Trichoderma harzianum-Purpureocillium lilacinum complex microbial inoculant and lactic acid bacteria into the first molasses liquid, and carrying out first fermentation to obtain a first fermentation liquid; mixing the first fermentation liquor and the second molasses liquor to obtain mixed molasses liquor; adding bacillus subtilis into the mixed molasses liquid, and carrying out second fermentation to obtain second fermentation liquid; and sequentially adding a nitrogen fertilizer, a phosphate fertilizer and a potash fertilizer into the second fermentation liquor to obtain the water-soluble fertilizer containing the microorganism potassium fulvate. The water-soluble fertilizer containing microorganism potassium fulvate prepared by the preparation method provided by the invention can effectively improve the yield and quality of crops, improve the soil structure, enhance the soil fertility and promote the root development of the crops.

Description

Technical Field

[0001] The present invention relates to the technical field of fertilizers, in particular to a water-soluble fertilizer containing microbial potassium fulvic acid and a preparation method thereof. Background Art

[0002] In agricultural production, the selection and application of fertilizers has always been a key factor influencing crop yield and quality. Traditional agricultural production often relies on chemical fertilizers. While these fertilizers can significantly increase crop growth in the short term, long-term use not only leads to soil degradation but also exacerbates environmental pollution. As the concept of sustainable development becomes more popular, finding and utilizing renewable and environmentally friendly fertilizer resources has become a key area of ​​agricultural scientific research. In this context, waste resource utilization has become a highly promising field, especially for industrial waste that was once considered a burden, such as molasses, the waste liquid from sugar factory production.

[0003] Molasses, a byproduct of the sugar industry, is rich in organic matter, trace elements, and nutrients that can be absorbed and utilized by crops. In theory, it is a high-quality organic fertilizer raw material. However, the direct application of untreated molasses in agricultural production presents numerous problems. First, the high concentration of sugar and other organic matter in molasses decomposes slowly in the soil and attracts pests, which is detrimental to the healthy growth of crops. Second, not all nutrients in molasses are suitable for direct absorption by crops; they require appropriate fermentation to convert them into a form that is more easily absorbed by plant roots. Furthermore, the direct discharge of untreated molasses can pollute the environment and affect the ecological balance of aquatic bodies. Summary of the Invention

[0004] In order to solve the above-mentioned deficiencies in the prior art, the present invention provides a water-soluble fertilizer containing microbial potassium fulvic acid and a preparation method thereof;

[0005] In order to solve the above technical problems, one of the technical solutions provided by the present invention is as follows:

[0006] A method for preparing a water-soluble fertilizer containing microbial potassium humate comprises the following steps:

[0007] providing a first molasses liquid and a second molasses liquid;

[0008] adding a composite bacterial agent of Trichoderma harzianum and Pseudomonas lilacinus and lactic acid bacteria to the first molasses liquid for a first fermentation to obtain a first fermentation liquid;

[0009] mixing the first fermentation liquid and the second molasses liquid to obtain a mixed molasses liquid;

[0010] adding Bacillus subtilis to the mixed molasses liquid for a second fermentation to obtain a second fermentation liquid;

[0011] Nitrogen fertilizer, phosphorus fertilizer and potassium fertilizer are added to the second fermentation liquid in sequence to obtain a water-soluble fertilizer containing microbial potassium humate.

[0012] In one embodiment, the mass ratio of the first molasses liquid to the second molasses liquid is 1:1.

[0013] In one embodiment, the mass ratio of the sum of the first molasses liquid and the second molasses liquid to the Trichoderma harzianum-Purplesporium lilacinum composite inoculum is 1000:1; and / or

[0014] The mass ratio of the sum of the first molasses liquid and the second molasses liquid to the lactic acid bacteria is 1800:1.

[0015] In one embodiment, the mass ratio of the sum of the first molasses liquid and the second molasses liquid to the Bacillus subtilis is 200:1.

[0016] In one embodiment, the temperature of the first fermentation is 20-30° C., and the time of the first fermentation is 7 days; and / or

[0017] The temperature of the second fermentation is 25-30° C., and the time of the second fermentation is 7 days.

[0018] In one embodiment, the mass ratio of the sum of the first molasses liquid and the second molasses liquid to the nitrogen fertilizer is (25:4)-(10:1);

[0019] Preferably, the mass ratio of the sum of the first molasses liquid and the second molasses liquid to the nitrogen fertilizer is 25:4;

[0020] Preferably, the nitrogen fertilizer is selected from any one of nitrate nitrogen, ammonium nitrogen, and amide nitrogen, or a combination of at least two of them.

[0021] In one embodiment, the mass ratio of the sum of the first molasses liquid and the second molasses liquid to the phosphate fertilizer is (20:1)-(50:1);

[0022] Preferably, the mass ratio of the sum of the first molasses liquid and the second molasses liquid to the phosphate fertilizer is 20:1;

[0023] Preferably, the phosphate fertilizer is selected from any one of monoammonium phosphate, diammonium phosphate, and potassium dihydrogen phosphate, or a combination of at least two thereof.

[0024] In one embodiment, the mass ratio of the sum of the first molasses liquid and the second molasses liquid to the potash fertilizer is 100:3;

[0025] Preferably, the potash fertilizer is selected from any one of potassium chloride, potassium sulfate, and potassium nitrate, or a combination of at least two of them.

[0026] In one embodiment, the phosphorus fertilizer and the potassium fertilizer are added in sequence at least 2 hours after the nitrogen fertilizer is added.

[0027] The second technical solution provided by the present invention is as follows:

[0028] A water-soluble fertilizer containing microbial potassium humate prepared according to the method described above.

[0029] Based on the above, compared with the existing technology, the water-soluble fertilizer containing microbial potassium humate prepared by the preparation method provided by the present invention can effectively improve the yield and quality of crops, improve soil structure, enhance soil fertility, and promote the development of crop roots.

[0030] Other features and beneficial effects of the present invention will be described in the following description, and in part will become apparent from the description, or will be understood through practice of the present invention. The objectives and other beneficial effects of the present invention can be achieved and obtained through the structures particularly pointed out in the description and claims. DETAILED DESCRIPTION

[0031] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments; the technical features designed in the different implementation modes of the present invention described below can be combined with each other as long as they do not conflict with each other; based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0032] In the description of the present invention, it should be noted that all terms used in the present invention (including technical terms and scientific terms) have the same meanings as those generally understood by ordinary technicians in the field to which the present invention belongs, and should not be understood as limiting the present invention; it should be further understood that the terms used in the present invention should be understood to have meanings consistent with the meanings of these terms in the context of this specification and in the relevant fields, and should not be understood in an idealized or overly formal sense, unless explicitly defined as such in the present invention.

[0033] One embodiment of the present invention provides a method for preparing a water-soluble fertilizer containing microbial potassium humate, comprising the following steps:

[0034] Step 1, providing a first molasses liquid and a second molasses liquid;

[0035] In a preferred embodiment, the mass ratio of the first molasses liquid to the second molasses liquid is 1:1. This is because molasses is an important carbon source in the fermentation process, and its addition amount directly affects the fermentation effect. By adding equal amounts of molasses liquid in two batches, the carbon source supply during the fermentation process can be better controlled, avoiding the problem of adding too much molasses at once, which would result in an excessively high concentration in the early fermentation stage and affect the growth and metabolism of microorganisms.

[0036] Step 2: adding a composite bacterial agent of Trichoderma harzianum and Pseudomonas lilacinus and lactic acid bacteria to the first molasses liquid to perform a first fermentation to obtain a first fermentation liquid;

[0037] In this step, a composite bacterial agent of Trichoderma harzianum and Psoralea lilacinus and lactic acid bacteria are added to the first molasses liquid. Trichoderma harzianum is an important biocontrol bacterium with antagonistic effects that can inhibit the growth of various pathogens. It can reduce the infection of pathogens by secreting antimicrobial substances, competing for nutrients and space, etc. Psoralea lilacinus has a significant inhibitory effect on harmful organisms such as root-knot nematodes and can also promote plant growth; when Trichoderma harzianum and Psoralea lilacinus are used in combination, they can work synergistically, not only preventing and controlling diseases, but also promoting plant growth and improving crop stress resistance; and lactic acid bacteria can reduce the pH value of the fermentation liquid during the fermentation process, creating an acidic environment, inhibiting the growth of harmful microorganisms, and producing organic acids such as lactic acid, which helps subsequent fermentation.

[0038] In a preferred embodiment, the temperature of the first fermentation is 20-30°C, and the time of the first fermentation is 7 days;

[0039] In some preferred embodiments, the mass ratio of the sum of the first molasses liquid and the second molasses liquid to the Trichoderma harzianum-Purplesporium lilacinum composite inoculum is 1000:1; and / or the mass ratio of the sum of the first molasses liquid and the second molasses liquid to the lactic acid bacteria is 1800:1.

[0040] Step 3: mixing the first fermentation liquid and the second molasses liquid to obtain a mixed molasses liquid;

[0041] In this step, first, the unfermented molasses liquid is rich in carbon sources and nutrients. Mixing it with the molasses liquid after the first fermentation can provide continuous nutritional support for microorganisms and avoid fermentation stagnation caused by carbon source depletion; secondly, the addition of unfermented molasses liquid can adjust the physical and chemical properties of the fermentation liquid, such as pH and osmotic pressure, to create a more stable environment for the growth and metabolism of microorganisms; thirdly, the mixed fermentation liquid provides richer substrates and metabolites for different microorganisms, promoting synergistic metabolism among microorganisms. For example, the organic acids produced by lactic acid bacteria during the fermentation process can lower the pH value, creating more favorable growth conditions for Trichoderma harzianum and Trichoderma lilacinum; finally, through mixed fermentation, microorganisms can use the nutrients in the unfermented molasses liquid to further synthesize bioactive ingredients such as antibacterial substances and enzymes, thereby improving the biological control effect of the fermentation liquid;

[0042] Specifically, Trichoderma harzianum produces a variety of antimicrobial substances during fermentation. A secondary mixing with unfermented molasses provides a more abundant carbon source, enhancing its ability to inhibit pathogens. During fermentation, Pseudomonas lilacinus requires a suitable carbon source and environmental conditions to synthesize bioactive substances such as chitinase. The addition of unfermented molasses optimizes these conditions and promotes its metabolic activity. Lactic acid bacteria produce lactic acid in the early stages of fermentation, lowering the pH and creating an acidic environment for subsequent microbial growth. The addition of unfermented molasses further supports the growth of lactic acid bacteria and maintains the acidic environment of the fermentation broth.

[0043] Step 4: adding Bacillus subtilis to the mixed molasses liquid for a second fermentation to obtain a second fermentation liquid;

[0044] Adding a second molasses solution also provides a sufficient carbon source for Bacillus subtilis, promoting its growth and metabolism. Bacillus subtilis is a common beneficial microorganism that produces a variety of enzymes, antimicrobial substances, and plant growth hormones. During the second fermentation, it utilizes the remaining molasses solution to multiply, producing enzymes such as proteases and amylases, which help degrade organic matter and increase the availability of nutrients in the fermentation product. It also produces spores, enhancing the product's resistance and stability, facilitating storage and use. Furthermore, some of its secreted antimicrobial substances can further inhibit the growth of harmful microorganisms, synergizing with the microorganisms in the first fermentation to enhance the inhibitory effect on plant pathogens and promote plant growth.

[0045] In a preferred embodiment, the temperature of the second fermentation is 25-30°C, and the time of the second fermentation is 7 days;

[0046] In a preferred embodiment, the mass ratio of the sum of the first molasses liquid and the second molasses liquid to the Bacillus subtilis is 200:1.

[0047] Step 5: Add nitrogen fertilizer, phosphorus fertilizer and potassium fertilizer to the second fermentation liquid in sequence to obtain a water-soluble fertilizer containing microbial potassium humate.

[0048] Specifically, nitrogen fertilizer can promote the growth of plant branches and leaves, increase chlorophyll content, improve plant photosynthesis efficiency, make plants luxuriant, and improve quality indicators such as protein content of agricultural products; phosphorus fertilizer plays an important role in plant root development, flowering and fruiting, and can promote early flowering and early fruiting of plants, improve the quality and yield of fruits, and enhance plant resistance to stress, such as drought and cold resistance; potassium fertilizer helps plant stems to be strong, enhances the plant's resistance to lodging, promotes the plant's absorption and conversion of nutrients such as nitrogen and phosphorus, improves the taste, color and other qualities of agricultural products, and can also enhance the plant's disease resistance; in combination with fermentation The microorganisms in the liquid cooperate with each other. The microorganisms can decompose organic matter in the soil and release more nutrients for plants to absorb. At the same time, nitrogen, phosphorus and potassium fertilizers provide additional nutrients for the growth and reproduction of microorganisms, promote the activity of microorganisms, and enable them to better play their roles in improving the soil and inhibiting harmful microorganisms. At the same time, potassium humate itself has the functions of improving the soil and stimulating plant growth. After adding nitrogen, phosphorus and potassium fertilizers, the water-soluble fertilizer containing microbial potassium humate can play a greater role in regulating soil pH, increasing soil water and fertilizer retention capacity, etc., and further promote plants to absorb and utilize nutrients.

[0049] In a preferred embodiment, the mass ratio of the sum of the first molasses liquid and the second molasses liquid to the nitrogen fertilizer is (25:4)-(10:1);

[0050] Preferably, the mass ratio of the sum of the first molasses liquid and the second molasses liquid to the nitrogen fertilizer is 25:4;

[0051] Preferably, the nitrogen fertilizer is selected from any one of nitrate nitrogen, ammonium nitrogen, and amide nitrogen, or a combination of at least two of them.

[0052] More preferably, the phosphorus fertilizer and the potassium fertilizer are added in sequence at least 2 hours after the nitrogen fertilizer is added.

[0053] One of the purposes of this operation is to avoid nutrient antagonism, such as nitrogen-phosphorus antagonism: a large amount of ammonium nitrogen will affect the plant's absorption of phosphorus. If nitrogen fertilizer and phosphorus fertilizer are added in large quantities at the same time, the ammonium ions in the soil may combine with phosphate ions to form insoluble ammonium phosphate salts, which will reduce the effectiveness of phosphorus and affect the plant's absorption of phosphorus. Interval addition can reduce this antagonistic effect, allowing phosphorus to be better absorbed and utilized by plants; for example, nitrogen-potassium antagonism: excessive potassium ions will inhibit the plant's absorption of ammonium ions, and high concentrations of ammonium nitrogen will also affect the absorption of potassium ions. Adding nitrogen fertilizer first, allowing the plant to have a certain amount of time to absorb and utilize some of the nitrogen, and then adding potassium fertilizer can reduce the antagonistic effect between the two and ensure the normal absorption of nitrogen and potassium by the plant;

[0054] Furthermore, microorganisms have different nutrient requirements at different stages. Adding nitrogen fertilizer first provides the nitrogen source they need for growth and reproduction, promoting their proliferation and metabolic activity. Over time, the microbial metabolism alters the soil environment, and adding phosphorus and potassium fertilizers at this stage facilitates further metabolism and soil nutrient conversion by microorganisms.

[0055] The demand for nitrogen, phosphorus, and potassium during plant growth follows a certain order and in different proportions. Generally, the demand for nitrogen is relatively high in the early stages of growth to promote the growth of branches and leaves. As the growth process progresses, the demand for phosphorus and potassium gradually increases for root development, flowering and fruiting, etc. Adding nitrogen first, then phosphorus and potassium at intervals is more in line with the staged nutrient demand of plant growth, can improve fertilizer utilization, and promote healthy plant growth.

[0056] Furthermore, ammonium nitrogen or urea in nitrogen fertilizers require time to transform in the soil. For example, urea needs to be converted to ammonium nitrogen by the action of urease before it can be better absorbed by plants and adsorbed and fixed in the soil. Adding nitrogen fertilizer first and then waiting for a while facilitates its initial transformation and stabilization in the soil. Adding phosphorus and potassium fertilizers later allows all three fertilizers to be gradually released and utilized in the soil, reducing nutrient loss and volatilization and improving the overall effectiveness and utilization of the fertilizers.

[0057] In a preferred embodiment, the mass ratio of the sum of the first molasses liquid and the second molasses liquid to the phosphate fertilizer is (20:1)-(50:1);

[0058] Preferably, the mass ratio of the sum of the first molasses liquid and the second molasses liquid to the phosphate fertilizer is 20:1;

[0059] Preferably, the phosphate fertilizer is selected from any one of monoammonium phosphate, diammonium phosphate, and potassium dihydrogen phosphate, or a combination of at least two thereof.

[0060] In a preferred embodiment, the mass ratio of the sum of the first molasses liquid and the second molasses liquid to the potash fertilizer is 100:3;

[0061] Preferably, the potash fertilizer is selected from any one of potassium chloride, potassium sulfate, and potassium nitrate, or a combination of at least two of them.

[0062] Step 6: Pour the prepared water-soluble fertilizer containing microbial potassium humate into barrels, and then place it at room temperature of 15-30℃ for at least 3 days before use.

[0063] In this step, the prepared potassium fulvate-containing water-soluble fertilizer contains a variety of active microorganisms, such as Trichoderma harzianum and Bacillus subtilis. These microorganisms may enter a dormant state due to environmental changes during the production process. Leaving the fertilizer at room temperature for three days provides a suitable environment for the microorganisms, allowing them to gradually regain activity, thereby better exerting their growth-promoting and disease-preventing functions. Furthermore, potassium fulvate itself has good stability, but after mixing with other fertilizer components, it requires time to reach optimal chemical and physical equilibrium. Leaving the fertilizer at room temperature for three days ensures that nutrients such as nitrogen, phosphorus, and potassium in the fertilizer fully bind to the potassium fulvate, forming a more stable complex and improving fertilizer utilization. Furthermore, potassium fulvate is rich in various active groups, providing carbon and nitrogen sources for microorganisms, promoting their reproduction and metabolism. Leaving the fertilizer at room temperature for three days allows the microorganisms to fully contact the potassium fulvate, exerting a synergistic effect and further improving the fertilizer's efficiency. Finally, leaving the fertilizer at room temperature for a period of time can buffer environmental changes such as temperature and pH that may occur during the production process, reducing damage to the microorganisms and maintaining their activity. This is crucial to improving the effectiveness of fertilizer applications in the field.

[0064] During the initial testing process, the present invention collected the molasses liquid and subjected it to a one-time fermentation process in an attempt to obtain an organic fertilizer that can be used in agriculture. However, the test results show that this method of adding all the molasses liquid at once for fermentation is not ideal. On the one hand, due to the extremely high content of sugar and other organic substances in the molasses liquid, a one-time large-scale fermentation system can easily lead to a fermentation process that is difficult to control, low fermentation efficiency, and even the production of undesirable fermentation products, such as alcohol and acetic acid, which are harmful to crop growth. On the other hand, it is difficult to fully convert all the nutrients in the molasses liquid during one-time fermentation, which greatly reduces the fertilizer efficiency of the final product and the crop's absorption efficiency of the fertilizer is still not high.

[0065] In view of this, the present invention proposes an innovative solution, namely, using molasses produced by a sugar factory as raw material, adding this unfermented molasses in batches and adding different fermentation strains in different steps to prepare a new water-soluble fertilizer containing microbial potassium humate. Specifically, the process first ferments a portion of the molasses, using a composite inoculant of Trichoderma harzianum and Pseudomonas lilacinus and lactic acid bacteria for a primary fermentation. These strains can effectively decompose sugars and other organic matter, while producing intermediate metabolites that are beneficial to crop growth. As the initial fermentation progresses, the remaining molasses is added in a timely manner, supplemented with Bacillus subtilis, to meet the growth requirements of microorganisms at different stages, ensuring the continuity and efficiency of the fermentation process.

[0066] The advantage of the step-by-step addition strategy is that it can effectively control the fermentation process, avoid fermentation runaway and nutrient loss caused by a one-time large-scale addition, and enable the organic matter in the molasses liquid to be more fully and evenly converted into nutrients that are easily absorbed by crops. In addition, after the fermentation is completed, the present invention also adds inorganic fertilizer components such as N, P, and K according to the crop growth requirements to prepare a water-soluble fertilizer containing microbial potassium humate. This fertilizer not only greatly improves the utilization rate of the molasses liquid, realizes the high-value utilization of waste, reduces pollution to the environment, but also significantly reduces the production cost of the enterprise.

[0067] In practical applications, this new water-soluble fertilizer has demonstrated remarkable fertilizing effects. It effectively increases crop yield and quality, improves soil structure and fertility, promotes root development, and enhances crop resistance to adverse conditions. Furthermore, due to its easy solubility and absorption, it reduces nutrient loss and improves fertilizer utilization, thus bringing significant economic and environmental benefits to agricultural production.

[0068] The beneficial effects of the present application will be described below with reference to specific embodiments and comparative examples.

[0069] Example 1

[0070] This embodiment provides a method for preparing a water-soluble fertilizer containing microbial potassium humate, comprising the following steps:

[0071] Step 1: providing a first molasses liquid and a second molasses liquid, wherein the mass ratio of the first molasses liquid to the second molasses liquid is 1:1. In this embodiment, the first molasses liquid is 5000 kg and the second molasses liquid is 5000 kg.

[0072] Step 2: Add Trichoderma harzianum·Purple spore compound bacteria and lactic acid bacteria to the first molasses liquid for a first fermentation to obtain a first fermentation liquid; the temperature of the first fermentation is 20-30°C, and the time of the first fermentation is 7 days; the mass ratio of the sum of the first molasses liquid and the second molasses liquid to the Trichoderma harzianum·Purple spore compound bacteria is 1000:1; the Trichoderma harzianum·Purple spore compound bacteria adopts Trichoderma harzianum·Purple spore (produced by Law's AgroSciences, with an effective viable count of ≥500 million / g); the mass ratio of the sum of the first molasses liquid and the second molasses liquid to the lactic acid bacteria is 1800:1.

[0073] Step 3: Mix the first fermentation liquid and the second molasses liquid, and stir the mixture for 20 minutes to obtain a mixed molasses liquid.

[0074] Step 4: Add Bacillus subtilis to the mixed molasses liquid for a second fermentation to obtain a second fermentation liquid; the temperature of the second fermentation is 25-30° C., and the time of the second fermentation is 7 days; the mass ratio of the sum of the first molasses liquid and the second molasses liquid to the Bacillus subtilis is 200:1; the Bacillus subtilis is produced by Shandong Weilan Biotechnology Co., Ltd.

[0075] Step 5: Add nitrogen fertilizer, phosphate fertilizer, and potash fertilizer to the second fermentation broth in sequence to obtain a water-soluble fertilizer containing microbial potassium fulvate. Phosphorus fertilizer and potash fertilizer are added in sequence 2 hours after the nitrogen fertilizer is added; the mass ratio of the sum of the first and second molasses solutions to the nitrogen fertilizer is 10:1; the nitrogen fertilizer is a water-soluble polymorphic nitrogen fertilizer (produced by Xinjiang Yuxiang Populus euphratica Chemical Co., Ltd., N-P2O5-K2O: 40-0-0); the mass ratio of the sum of the first and second molasses solutions to the phosphate fertilizer is 50:1; the phosphate fertilizer is monoammonium phosphate (produced by Yuntu New Energy Materials (Jingzhou) Co., Ltd., N-P2O5-K2O: 12-61-0); the mass ratio of the sum of the first and second molasses solutions to the potash fertilizer is 100:3; the potash fertilizer is fully water-soluble agricultural potassium sulfate (produced by State-owned Investment Xinjiang Lop Nur Potash Co., Ltd., with a water-soluble potassium oxide (K2O) content ≥ 53.8%).

[0076] Step 6: Put the prepared water-soluble fertilizer containing microbial potassium humate into barrels, and then place it at room temperature of 25°C for 3 days.

[0077] Example 2

[0078] This embodiment provides a method for preparing a water-soluble fertilizer containing microbial potassium humate, comprising the following steps:

[0079] Step 1: providing a first molasses liquid and a second molasses liquid, wherein the mass ratio of the first molasses liquid to the second molasses liquid is 1:1. In this embodiment, the first molasses liquid is 5000 kg and the second molasses liquid is 5000 kg.

[0080] Step 2: Add Trichoderma harzianum·Purple spore compound bacteria and lactic acid bacteria to the first molasses liquid for a first fermentation to obtain a first fermentation liquid; the temperature of the first fermentation is 20-30°C, and the time of the first fermentation is 7 days; the mass ratio of the sum of the first molasses liquid and the second molasses liquid to the Trichoderma harzianum·Purple spore compound bacteria is 1000:1; the Trichoderma harzianum·Purple spore compound bacteria adopts Trichoderma harzianum·Purple spore (produced by Law's AgroSciences, with an effective viable count of ≥500 million / g); the mass ratio of the sum of the first molasses liquid and the second molasses liquid to the lactic acid bacteria is 1800:1.

[0081] Step 3: Mix the first fermentation liquid and the second molasses liquid, and stir the mixture for 20 minutes to obtain a mixed molasses liquid.

[0082] Step 4: Add Bacillus subtilis to the mixed molasses liquid for a second fermentation to obtain a second fermentation liquid; the temperature of the second fermentation is 25-30° C., and the time of the second fermentation is 7 days; the mass ratio of the sum of the first molasses liquid and the second molasses liquid to the Bacillus subtilis is 200:1; the Bacillus subtilis is produced by Shandong Weilan Biotechnology Co., Ltd.

[0083] Step 5: Add nitrogen fertilizer, phosphate fertilizer, and potash fertilizer to the second fermentation liquid in sequence to obtain a water-soluble fertilizer containing microbial potassium fulvate. Phosphorus fertilizer and potash fertilizer are added in sequence 2 hours after the nitrogen fertilizer is added; the mass ratio of the sum of the first and second molasses liquids to the nitrogen fertilizer is 50:7; the nitrogen fertilizer is a water-soluble polymorphic nitrogen fertilizer (produced by Xinjiang Yuxiang Populus euphratica Chemical Co., Ltd., N-P2O5-K2O: 40-0-0); the mass ratio of the sum of the first and second molasses liquids to the phosphate fertilizer is 100:3; the phosphate fertilizer is monoammonium phosphate (produced by Yuntu New Energy Materials (Jingzhou) Co., Ltd., N-P2O5-K2O: 12-61-0); the mass ratio of the sum of the first and second molasses liquids to the potash fertilizer is 100:3; the potash fertilizer is fully water-soluble agricultural potassium sulfate (produced by State-owned Investment Xinjiang Lop Nur Potash Co., Ltd., water-soluble potassium oxide (K2O) ≥ 53.8%).

[0084] Step 6: Put the prepared water-soluble fertilizer containing microbial potassium humate into barrels, and then place it at room temperature of 25°C for 3 days.

[0085] Example 3

[0086] This embodiment provides a method for preparing a water-soluble fertilizer containing microbial potassium humate, comprising the following steps:

[0087] Step 1: providing a first molasses liquid and a second molasses liquid, wherein the mass ratio of the first molasses liquid to the second molasses liquid is 1:1. In this embodiment, the first molasses liquid is 5000 kg and the second molasses liquid is 5000 kg.

[0088] Step 2: Add Trichoderma harzianum·Purple spore compound bacteria and lactic acid bacteria to the first molasses liquid for a first fermentation to obtain a first fermentation liquid; the temperature of the first fermentation is 20-30°C, and the time of the first fermentation is 7 days; the mass ratio of the sum of the first molasses liquid and the second molasses liquid to the Trichoderma harzianum·Purple spore compound bacteria is 1000:1; the Trichoderma harzianum·Purple spore compound bacteria adopts Trichoderma harzianum·Purple spore (produced by Law's AgroSciences, with an effective viable count of ≥500 million / g); the mass ratio of the sum of the first molasses liquid and the second molasses liquid to the lactic acid bacteria is 1800:1.

[0089] Step 3: Mix the first fermentation liquid and the second molasses liquid, and stir the mixture for 20 minutes to obtain a mixed molasses liquid.

[0090] Step 4: Add Bacillus subtilis to the mixed molasses liquid for a second fermentation to obtain a second fermentation liquid; the temperature of the second fermentation is 25-30° C., and the time of the second fermentation is 7 days; the mass ratio of the sum of the first molasses liquid and the second molasses liquid to the Bacillus subtilis is 200:1; the Bacillus subtilis is produced by Shandong Weilan Biotechnology Co., Ltd.

[0091] Step 5: Add nitrogen fertilizer, phosphate fertilizer, and potash fertilizer to the second fermentation liquid in sequence to obtain a water-soluble fertilizer containing microbial potassium fulvate. Phosphorus fertilizer and potash fertilizer are added in sequence 2 hours after the nitrogen fertilizer is added; the mass ratio of the sum of the first and second molasses liquids to the nitrogen fertilizer is 50:7; the nitrogen fertilizer is a water-soluble polymorphic nitrogen fertilizer (produced by Xinjiang Yuxiang Populus euphratica Chemical Co., Ltd., N-P2O5-K2O: 40-0-0); the mass ratio of the sum of the first and second molasses liquids to the phosphate fertilizer is 100:3; the phosphate fertilizer is monoammonium phosphate (produced by Yuntu New Energy Materials (Jingzhou) Co., Ltd., N-P2O5-K2O: 12-61-0); the mass ratio of the sum of the first and second molasses liquids to the potash fertilizer is 100:3; the potash fertilizer is fully water-soluble agricultural potassium sulfate (produced by State-owned Investment Xinjiang Lop Nur Potash Co., Ltd., water-soluble potassium oxide (K2O) ≥ 53.8%).

[0092] Step 6: Put the prepared water-soluble fertilizer containing microbial potassium humate into barrels, and then place it at room temperature of 25°C for 3 days.

[0093] Comparative Example 1

[0094] The difference between this comparative example and Example 3 is that the first molasses liquid and the second molasses liquid are mixed and then the Trichoderma harzianum-Purpleurosporium complex bacterial agent, lactic acid bacteria and Bacillus subtilis are directly added thereto.

[0095] Test Case

[0096] (1) Determination of soybean agronomic traits, yield and component factors

[0097] During the soybean maturity period, in each plot (plot area of ​​666.7m 2 , 18,000 plants per mu, machine sowing; all treatments were applied with root water at 5,000 ml / mu each time during the soybean seedling, flowering and grain-filling stages, and 150 ml / mu was sprayed on the leaves. Other management conditions were the same as conventional cultivation. Ten plants with uniform growth and representativeness were selected from each area, the whole plants were dug out, labeled and brought back to the laboratory for determination of agronomic traits, yield components, quality and soil fertility. Agronomic traits such as plant height, bottom pod height, stem diameter, and number of effective branches were measured with a tape measure and vernier caliper, and grain weight per plant and 100-grain weight were measured with an electronic scale. Fat content was determined using the first method of GB 5009.6-2016.

[0098] Table 1 Effects on soybean growth

[0099]

[0100] Compared with the control example, the embodiment can significantly increase the soybean stem thickness, increase the number of soybean main stem nodes, and increase the number of effective branches; among them, the stem thickness of Example 3 is the largest, 1.10 cm higher than the control, the number of soybean main stem nodes of Example 1 is the highest, which is 14.9, and the number of effective branches of Example 2 is the highest, which is 0.5. Among all the treatments, Example 1 has the highest plant height, which is significantly higher than other examples.

[0101] Table 2 Effects on soybean quality, yield and component factors

[0102] Number of clips per plant Low pod height (cm) Number of seeds per plant Number of grains per pod Example 1 28.10±6.56ab 32.60±8.86a 60.90±11.57bc 2.19±0.16a Example 2 37.40±16.67a 27.60±6.87ab 90.40±36.10a 2.44±0.17a Example 3 37.40±12.68a 27.30±4.69ab 87.60±33.53a 2.32±0.24a Comparative Example 1 22.15±12.54b 25.12±4.57b 50.28±15.20c 2.34±0.27a Single plant grain weight (g) 100-grain weight (g) Equivalent yield per mu (kg) Fat (g / 100g) Example 1 8.39±1.22b 16.65±0.07b 123.70±40.09b 13.73±0.21b Example 2 15.02±7.37a 18.00±0.38a 229.76±112.73a 15.73±0.31a Example 3 14.96±5.77a 18.44±0.70a 228.87±88.33a 15.63±0.25a Comparative Example 1 8.62±1.41c 16.59±0.27b 123.81±42.13b 13.28±0.22c

[0103] Compared with the comparative example, the embodiment can significantly increase the number of soybean clips per plant, the grain weight per plant, the yield per mu, etc.; among them, the number of soybean clips per plant, the grain weight per plant, the yield per mu and the number of grains per plant of Example 2 are the highest, which are increased by 6.4, 16.37g, 105.95kg and 40.12 respectively compared with the comparative example 1.

[0104] (2) Determination of soil nutrient content

[0105] Soil fertility was determined in each treatment before soybean harvest and winter plowing. Soil samples were collected from the 0-40 cm soil layer using a five-point sampling method similar to the method of Ma Mingze et al. Soil organic matter content was determined using the potassium dichromate external heating method, total nitrogen was determined using the semi-micro Kjeldahl method, total potassium was determined using the sodium hydroxide fusion method, available phosphorus content was determined using sodium bicarbonate extraction and the molybdenum antimony colorimetric method, available potassium was determined using atomic absorption spectrometry, nitrate nitrogen was determined using a flow analyzer, soil pH was measured using a pH meter, and soil EC was measured using an EC meter (Ma Mingze). Available zinc and iron content in the soil was determined using the atomic absorption spectrometry method according to NY / T 890-2004.

[0106] Table 3 Effects on soybean soil nutrients

[0107]

[0108] Compared with the comparative example, the embodiments can significantly increase the nutrient content in the soil, which is beneficial to the growth and development of soybeans; among them, the soil organic matter content of Example 3 is the highest, followed by Example 2 and Example 1, and is increased by 2.37, 1.66, and 1.18 g / kg compared with Comparative Example 1.

[0109] In summary, compared with the prior art, the water-soluble fertilizer containing microbial potassium humate provided by the present invention can promote soybean growth, increase stem diameter, increase yield, and improve soybean quality; not only that, it can also improve soil fertility, increase soil organic matter content, reduce soil alkalinity, and increase soil nutrient content; at the same time, it can increase the SPAD value of new plum leaves, increase the N content of leaves, increase the protein, potassium, calcium, fructose and glucose content of fruits, and improve fruit quality.

[0110] In addition, those skilled in the art should understand that, although there are many problems in the prior art, each embodiment or technical solution of the present invention may be improved in only one or several aspects, without having to simultaneously solve all the technical problems listed in the prior art or background art. Those skilled in the art should understand that any content not mentioned in a claim should not be construed as limiting the claim.

[0111] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the above embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for preparing a water-soluble fertilizer containing microbial potassium humate, characterized in that: The following steps are involved: providing a first molasses liquid and a second molasses liquid; adding a composite bacterial agent of Trichoderma harzianum and Pseudomonas lilacinus and lactic acid bacteria to the first molasses liquid for a first fermentation to obtain a first fermentation liquid; mixing the first fermentation liquid and the second molasses liquid to obtain a mixed molasses liquid; adding Bacillus subtilis to the mixed molasses liquid for a second fermentation to obtain a second fermentation liquid; Nitrogen fertilizer, phosphorus fertilizer and potassium fertilizer are added to the second fermentation liquid in sequence to obtain a water-soluble fertilizer containing microbial potassium humate.

2. The method for preparing a water-soluble fertilizer containing microbial potassium humate according to claim 1, wherein The mass ratio of the first molasses liquid to the second molasses liquid is 1:

1.

3. The preparation method of the microbial potassium fulvic acid water-soluble fertilizer according to claim 1, wherein The mass ratio of the sum of the first molasses liquid and the second molasses liquid to the Trichoderma harzianum-Purplesporium lilacinum composite bacterial agent is 1000:1; and / or The mass ratio of the sum of the first molasses liquid and the second molasses liquid to the lactic acid bacteria is 1800:

1.

4. The method for preparing a water-soluble fertilizer containing microbial potassium humate according to claim 1, wherein The mass ratio of the sum of the first molasses liquid and the second molasses liquid to the Bacillus subtilis is 200:

1.

5. The preparation method of the microbial potassium humate water-soluble fertilizer according to claim 1, wherein The temperature of the first fermentation is 20-30° C., and the time of the first fermentation is 7 days; and / or The temperature of the second fermentation is 25-30° C., and the time of the second fermentation is 7 days.

6. The method for preparing a water-soluble fertilizer containing microbial potassium humate according to claim 1, wherein: The mass ratio of the sum of the first molasses liquid and the second molasses liquid to the nitrogen fertilizer is (25:4)-(10:1); Preferably, the nitrogen fertilizer is selected from any one of nitrate nitrogen, ammonium nitrogen, and amide nitrogen, or a combination of at least two of them.

7. The method for preparing a water-soluble fertilizer containing microbial potassium humate according to claim 1, wherein: The mass ratio of the sum of the first molasses liquid and the second molasses liquid to the phosphate fertilizer is (20:1)-(50:1); Preferably, the phosphate fertilizer is selected from any one of monoammonium phosphate, diammonium phosphate, and potassium dihydrogen phosphate, or a combination of at least two thereof.

8. The method for preparing a water-soluble fertilizer containing microbial potassium humate according to claim 1, wherein: The mass ratio of the sum of the first molasses liquid and the second molasses liquid to the potash fertilizer is 100:3; Preferably, the potash fertilizer is selected from any one of potassium chloride, potassium sulfate, and potassium nitrate, or a combination of at least two of them.

9. The method for preparing a water-soluble fertilizer containing microbial potassium humate according to claim 1, wherein: After the nitrogen fertilizer is added, the phosphorus fertilizer and the potash fertilizer are added in sequence at least 2 hours apart.

10. A water-soluble fertilizer containing microbial potassium humate prepared according to the method according to any one of claims 1 to 9.

Images