Soil micro-plastic surface microorganism culture device

Abstract

The invention discloses a soil micro-plastic surface microorganism culture device, and belongs to the technical field of environmental microorganism culture.The culture device comprises a first tank body, a third tank body and a fourth tank body which are communicated with the first tank body are arranged on one side of the first tank body, and a conveying header pipe is arranged above the first tank body; the first tank body is communicated with the conveying main pipe through a first conveying pipe, the third tank body is communicated with the first tank body through a seventh conveying pipe and is communicated with the conveying main pipe through a third conveying pipe, the bottom of the fourth tank body is connected with a first pump body, and the first pump body is communicated with the seventh conveying pipe through a sixth conveying pipe. A culture environment simulation function of a soil-micro-plastic composite system is realized, the degree of freedom of environment parameter control is high, and soil environment conditions are truly simulated.

Description

Technical Field

[0001] The present invention relates to the technical field of environmental microorganism cultivation, and in particular to a soil microplastic surface microorganism cultivation device. Background Art

[0002] Microplastics are a ubiquitous emerging environmental pollutant. Due to their hydrophobic surface and large surface area, they promote biofilm formation and can serve as carriers for microbial colonization. Microplastics are small in size, lightweight, and difficult to degrade in the environment. They can migrate long distances through natural factors and human activities, posing a threat to the ecological environment and human health. They have garnered widespread attention both domestically and internationally in recent years. Research on microplastics has largely focused on aquatic ecosystems. As a widely used artificial polymer, most microplastics that enter water bodies are produced, used, and processed on land. Therefore, microplastics are more likely to first interact with microorganisms in the soil, altering the geochemical and biophysical environment and leading to environmental toxicity. Microplastics in the soil environment have a significant impact on the physical and chemical properties of the soil and the microbial community. In the process of remediating soil pollution, microbial cultivation is one of the essential steps. For this purpose, the prior art has proposed a variety of microbial cultivation equipment, such as the prior art KR102718790B1, which relates to a rapid microbial incubator. More specifically, it relates to a rapid microbial incubator that prevents the adsorption of foreign matter such as the culture material on the surface of the light irradiation device by arranging a light irradiation device in the incubation chamber and causing the culture material to form a rotating flow around the light irradiation device and stir the culture material, thereby preventing the light irradiation device from adsorbing foreign matter such as the culture material on the surface while being controlled in real time by a control unit. This technology installs the light irradiation device with LED lighting vertically from top to bottom in the main body of the rapid incubator to irradiate sufficient light during microbial cultivation. However, this solution still has room for improvement in terms of the simulation function of the culture environment of the soil-microplastic composite system. Summary of the Invention

[0003] The purpose of the present invention is to provide a soil microplastic surface microorganism cultivation device, which realizes the cultivation environment simulation function of the soil-microplastic composite system, has a high degree of freedom in controlling environmental parameters, and truly simulates soil environmental conditions.

[0004] In order to solve the above technical problems, the present invention specifically provides the following technical solutions: a soil microplastic surface microorganism cultivation device, comprising a first tank body, a third tank body and a fourth tank body connected to the first tank body are provided on one side of the first tank body, a delivery main pipe is provided above the first tank body, the first tank body and the delivery main pipe are connected through the first delivery pipe, the third tank body is connected to the first tank body through the seventh delivery pipe, the third tank body is connected to the delivery main pipe through the third delivery pipe, the bottom of the fourth tank body is connected to a first pump body, and the first pump body is connected to the seventh delivery pipe through the sixth delivery pipe. The first tank body is used to provide an environment for the growth of microorganisms. Soil and microplastics are placed inside it to form a soil-microplastic culture environment, realizing the culture environment simulation function of the soil-microplastic composite system. On this basis, the third tank body is set to supply air to the inside of the first tank body to simulate the gas supply in the natural soil environment. The fourth tank body is used to extract the discharged medium from the first tank body, such as water, gas, water-gas mixture, etc., which are extracted and temporarily stored in the fourth tank body. The setting of the delivery main pipe and the first delivery pipe realizes the provision of multi-angle medium input direction for the upper part of the first tank body, and also simulates the impact of weather conditions such as rain on the soil in the natural environment. Furthermore, the first pump body is provided to extract the medium inside the fourth tank body and send it into the seventh delivery pipe to mix with the internal gas to form a gas-liquid mixture and re-input it into the first tank body, solving the problem that directly introducing dry gas will cause water loss in the soil or culture environment. After connecting the water channel, the gas can be pre-humidified to maintain the stable humidity in the incubator, which helps to maintain the original state of the biofilm on the surface of the microplastics. In addition, this method recycles the discharged medium from the first tank body and reduces water waste.

[0005] According to one embodiment of the present invention, the fourth tank is connected to the first tank via a fourth delivery pipe, which in turn is connected to the main delivery pipe via a fifth delivery pipe. The output port of the fourth delivery pipe is located within the culture chamber of the first tank. This allows the medium to be pumped from the first tank in different directions, thereby regulating culture conditions within the first tank and preventing damage to the soil environment at the suction location due to excessive suction from a single direction.

[0006] According to one embodiment of the present invention, a second tank body is disposed outside the first tank body, and the second tank body is connected to the main delivery pipe via a second delivery pipe. The second tank body is used to input water required for cultivation or simulated rainfall environment into the first tank body. The water in the second tank body is pumped by a water pump inside the second tank body to the second delivery pipe and then to the main delivery pipe. The main delivery pipe can cooperate with the third delivery pipe to simultaneously input gas and water, providing multiple delivery modes. It can also perform suction through the main delivery pipe and the fifth delivery pipe, providing multiple control methods for cultivation environment conditions. The continuous operation of suction and medium input can achieve a self-cleaning effect on the inner wall of the main delivery pipe.

[0007] According to one embodiment of the present invention, the first tank body has a built-in partition plate, which divides the first tank body into a culture chamber and a collection chamber arranged vertically. There are at least two seventh delivery pipes, and the third tank body is connected to the culture chamber and the collection chamber respectively through the seventh delivery pipe. The role of the partition plate is not limited to spatial division. It can also cooperate with temperature control, ventilation, irrigation and other systems to accurately simulate natural or artificial environments. The partition plate can serve as a supporting layer to prevent soil collapse due to gravity or water flow, maintain porosity, and ensure the stability of gas exchange and water penetration. More importantly, the culture chamber and the collection chamber are arranged vertically and separated by the partition plate. This can prevent the mixing of liquids in the culture chamber and the collection chamber, and avoid the reverse migration of microorganisms, metabolites or pollutants.

[0008] According to one embodiment of the present invention, a medium distribution assembly connected to the first delivery pipe is provided above the culture chamber, and the medium distribution assembly includes a first diversion pipe connected to the output end of the first delivery pipe, a second diversion pipe is arranged in an array on the side of the first diversion pipe, and a nozzle is provided at the output port of the second diversion pipe. The internal flow area of ​​the connecting end of the first diverter pipe and the first delivery pipe is reduced toward the internal flow diameter of the other end thereof, and the internal flow area of ​​the connecting end of the second diverter pipe and the first diverter pipe is reduced toward the internal flow diameter of the other end thereof. The nozzle has a medium containing matrix, the interior of the matrix is ​​hollow, and the hollow part is connected to the second diverter pipe. Micropores are arranged in an array on the matrix, and the medium can be discharged through the micropores or enter the interior of the matrix through the micropores. The above means are used to solve the problem of pressure loss along the flow of liquid from the inlet to the end of the equal-diameter pipe due to friction resistance. The fluids entering the first diverter pipe and the second diverter pipe of the present invention maintain the characteristics of small difference in pressure and flow rate between the inlet and outlet ends, so that the final spraying amount is uniform, avoiding excessive moisture (such as the front end) or drought (such as the rear end) in local areas of the first tank body due to uneven pressure, and ensuring uniform humidity and nutrient distribution in the microbial culture environment.

[0009] When it is necessary to suck the medium inside the first tank body, the pump body inside the fourth tank body provides suction force, the other pipe openings of the delivery main pipe are closed, the fifth delivery pipe is kept connected with the first delivery pipe, and the medium enters the fifth delivery pipe in the reverse direction from the base of the nozzle. In this process, the pipe diameter design scheme of the above-mentioned first diversion pipe and the second diversion pipe can ensure balanced suction force at the nozzle end to avoid suction blockage problems.

[0010] According to one embodiment of the present invention, a flow guide is provided at the connection between the first diversion pipe and the first delivery pipe. The flow guide includes at least two coaxial flow guide pipes arranged inside and outside, which are connected by a connecting plate. A first filler is filled between the first diversion pipe and the flow guide. The first filler is arranged at intervals around the outer wall of the flow guide pipe adjacent to the inner wall of the first diversion pipe. The internally and externally distributed flow guide pipes and the connecting plate can transform the disordered inlet flow into a stable and symmetrical distribution state, guide the fluid step by step through the multi-stage cylindrical sleeve, make the flow velocity gradient transition smoothly, reduce turbulent kinetic energy, inhibit vortex formation, and thus improve spray uniformity. In addition, the internally and externally distributed flow guide pipes and the connecting plate enhance the shear effect of the fluid by changing the cross-sectional area and direction of the flow channel, which can promote the uniform dispersion of particles or bubbles when conveying mixed fluids, such as when conveying nutrient solution or water-gas mixture in a water body. In addition, the arrangement of the flow guide can reduce blockage at the connection between the first diversion pipe and the first delivery pipe, and the high shear force achieved by the flow guide can make the fluid flush the pipe wall and inhibit dirt adhesion.

[0011] According to one embodiment of the present invention, a stirring assembly is built into the culture chamber, and the stirring assembly includes a stirring shaft with spiral blades on the surface, stirring blades are arranged spirally around the outside of the stirring shaft, the stirring blades are connected to the stirring shaft through a stirring rod, and an auxiliary plate is provided at the end of the stirring shaft, and the auxiliary plate is connected to the stirring rod. One end of the auxiliary plate is connected to the stirring shaft, and the other end is connected to the stirring shaft. The coordination of the stirring shaft, the stirring blades and the stirring rod can achieve stirring to break up soil clumps, so that organic matter, microorganisms and added nutrients are evenly distributed, avoiding local nutrient excess or shortage. At the same time, the auxiliary plate provided at the end of the stirring shaft can expand the stirring range and continuously exchange the material at its end during the stirring process to improve the material stirring and movement effect, thereby avoiding the occurrence of heat accumulation problems in local areas and preventing microbial inactivation.

[0012] According to one embodiment of the present invention, a driving motor connected to the stirring shaft of the stirring assembly is built into the collecting chamber to realize the rotational movement of the stirring assembly.

[0013] According to one embodiment of the present invention, the partition plate is a perforated plate, specifically a mesh or grid-shaped partition plate that can support the soil and facilitate the discharge of excess water, media, etc. to simulate the displacement of water and gas in the soil in a natural environment.

[0014] According to one embodiment of the present invention, a feed port is provided at the upper end of the first tank body, which is used to feed materials such as soil, microplastics, nutrient solution, etc. At the same time, monitoring equipment such as sensors, CCD cameras, etc. can be taken into the first tank body through the feed port. A discharge pipe is provided at the lower end of the first tank body for discharging the medium.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows: the first tank body of the present invention is used to provide an environment for the growth of microorganisms, and soil and microplastics are placed inside it to form a soil-microplastic culture environment, thereby realizing the culture environment simulation function of the soil-microplastic composite system. The first pump body of the present invention extracts the medium inside the fourth tank body and sends it into the seventh delivery pipe to mix with the internal gas thereof to form a gas-liquid mixture which is then re-input into the first tank body, thereby solving the problem that directly introducing dry gas will cause water loss in the soil or culture environment. After connecting the water channel, the gas can be pre-humidified, which helps to maintain the original state of the biofilm on the surface of the microplastic. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] To more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for the embodiments or the description of the prior art. Obviously, the drawings described below are merely exemplary, and those skilled in the art can derive other implementation drawings based on the provided drawings without inventive effort.

[0017] Figure 1 This is a schematic diagram of a soil microplastic surface microorganism cultivation device of the present invention; Figure 2 This is a schematic diagram of the internal structure of the first tank body of the present invention; Figure 3 This is a schematic diagram of the water distribution component scheme of the present invention; Figure 4 Schematic diagram of the connection scheme between the first shunt pipe and the second shunt pipe of the present invention; Figure 5 Schematic diagram of the deflector solution of the present invention; Figure 6 This is a schematic diagram of the stirring assembly scheme of the present invention; Figure 7 This is a schematic diagram of another layout scheme of a soil microplastic surface microorganism cultivation device of the present invention in Example 2; Figure 8 for Figure 7 Top view of the device shown.

[0018] Explanation of the accompanying drawings: 10. First tank body; 11. First delivery pipe; 12. Discharge pipe; 13. Partition plate; 20. Second tank body; 21. Second delivery pipe; 22. Auxiliary pump body; 23. Delivery main pipe; 30. Third tank body; 31. Third delivery pipe; 32. Seventh delivery pipe; 40. Fourth tank body; 41. Fourth delivery pipe; 42. Fifth delivery pipe; 50. First pump body; 51. Sixth delivery pipe; 60. Medium distribution assembly; 61. First diverter pipe; 62. Guide; 621. First filling piece; 622. Connecting plate; 623. Guide pipe; 63. Second diverter pipe; 70. Stirring assembly; 71. Drive motor; 72. Stirring rod; 73. Stirring blade; 74. Auxiliary plate; 75. Stirring shaft. DETAILED DESCRIPTION

[0019] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0020] The following first describes the concepts involved in this application with reference to the accompanying drawings. It should be noted that the following description of each concept is intended only to make the content of this application easier to understand and does not limit the scope of protection of this application. At the same time, the embodiments and features in the embodiments of this application can be combined with each other unless there is a conflict. The following detailed description of this application will be made with reference to the accompanying drawings and in conjunction with the embodiments.

[0021] Example 1: Attached picture Figure 1 -Attached Figure 8As shown, the present invention provides a soil microplastic surface microorganism cultivation device, including a first tank body 10, a third tank body 30 and a fourth tank body 40 connected to the first tank body 10 are provided on one side of the first tank body 10, a delivery main pipe 23 is provided above the first tank body 10, the first tank body 10 and the delivery main pipe 23 are connected through a first delivery pipe 11, the third tank body 30 is connected to the first tank body 10 through a seventh delivery pipe 32, the third tank body 30 is connected to the delivery main pipe 23 through a third delivery pipe 31, and a first pump body 50 is connected to the bottom of the fourth tank body 40, and the first pump body 50 is connected to the seventh delivery pipe 32 through a sixth delivery pipe 51. The first tank body 10 is used to provide an environment for the growth of microorganisms. Soil and microplastics are placed inside it to form a soil microplastic culture environment, realizing the culture environment simulation function of the soil-microplastic composite system. On this basis, a third tank body 30 is set to supply air to the inside of the first tank body 10 to simulate the gas supply in a natural soil environment. The fourth tank body 40 is used to extract the discharged medium in the first tank body 10, such as water, gas, water-gas mixture, etc., which are temporarily stored in the fourth tank body 40 after being extracted. The arrangement of the delivery main pipe 30 and the first delivery pipe 11 realizes the multi-angle medium delivery to the upper part of the first tank body 10. Inlet direction, it also simulates the impact of weather conditions such as rain on the soil in a natural environment. Furthermore, the first pump body 50 is provided to extract the medium inside the fourth tank body 40 and send it into the seventh delivery pipe 32 to mix with the internal gas thereof to form a gas-liquid mixture which is re-input into the first tank body 10, thereby solving the problem that directly introducing dry gas will cause water loss in the soil or the culture environment. After connecting the waterway, the gas can be pre-humidified to maintain the stable humidity in the culture vessel, which helps to maintain the original state of the biofilm on the surface of the microplastics. In addition, this method recycles the medium discharged from the first tank body 10, reducing water waste.

[0022] The fourth tank 40 is connected to the first tank 10 via a fourth delivery pipe 41, which is in turn connected to the main delivery pipe 23 via a fifth delivery pipe 42. The output port of the fourth delivery pipe 41 is located within the culture chamber of the first tank 10. This allows the medium to be pumped from different directions within the first tank 10, regulating the culture conditions within the first tank 10 and preventing damage to the soil environment at the suction location due to excessive suction from a single direction.

[0023] A second tank body 20 is disposed outside the first tank body 10, and the second tank body 20 is connected to a main delivery pipe 23 via a second delivery pipe 21. The second tank body 20 is used to input water required for culture or simulated rainfall environment into the first tank body 10. The water in the second tank body 20 is pumped by a water pump inside the second tank body 20 to the second delivery pipe 21 and then to the main delivery pipe 23. The main delivery pipe 23 can cooperate with the third delivery pipe 31 to simultaneously input gas and water, providing multiple delivery modes. It can also perform suction through the main delivery pipe 23 and the fifth delivery pipe 42, providing multiple control methods for culture environment conditions. The continuous operation of suction and medium input can achieve a self-cleaning effect on the inner wall of the main delivery pipe 23.

[0024] The first tank body 10 has a built-in partition 13, which divides the first tank body 10 into a culture chamber and a collection chamber arranged one above the other. There are at least two seventh delivery pipes 32, and the third tank body 30 is connected to the culture chamber and the collection chamber respectively through the seventh delivery pipe 32. The role of the partition 13 is not limited to spatial division. It can also cooperate with temperature control, ventilation, irrigation and other systems to accurately simulate natural or artificial environments. The partition 13 can serve as a supporting layer to prevent soil collapse due to gravity or water flow, maintain porosity, and ensure the stability of gas exchange and water penetration. More importantly, the culture chamber and the collection chamber are arranged one above the other and separated by the partition 13. This can prevent the mixing of liquids in the culture chamber and the collection chamber, and prevent the reverse migration of microorganisms, metabolites or pollutants.

[0025] A medium distribution assembly 60 connected to the first delivery pipe 11 is provided above the culture chamber. The medium distribution assembly 60 includes a first diversion pipe 61 connected to the output end of the first delivery pipe 11. A second diversion pipe 63 is arranged in an array on the side of the first diversion pipe 61. The output port of the second diversion pipe 63 is provided with a nozzle. The internal flow area of ​​the end portion where the first diverter pipe 61 is connected to the first delivery pipe 11 is reduced toward the internal flow diameter of the other end portion; the internal flow area of ​​the end portion where the second diverter pipe 63 is connected to the first diverter pipe 61 is reduced toward the internal flow diameter of the other end portion; the nozzle has a medium containing base body, the interior of the base body is hollow, the hollow portion is connected to the second diverter pipe 63, micropores are arranged in an array on the base body, the medium can be discharged through the micropores or enter the interior of the base body through the micropores, and the above-mentioned means are used to solve the problem of pressure loss along the flow of liquid from the inlet to the end of the equal-diameter pipe due to friction resistance. The fluids entering the first diverter pipe 61 and the second diverter pipe 63 of the present invention maintain the characteristics of small difference in pressure and flow rate between the inlet and outlet ends, so that the final spraying amount is uniform, avoiding excessive moisture (such as the front end) or drought (such as the rear end) in local areas of the first tank body 10 due to uneven pressure, and ensuring uniform humidity and nutrient distribution in the microbial culture environment.

[0026] When it is necessary to suck the medium inside the first tank body 10, the pump body inside the fourth tank body 40 provides suction force, the other pipe openings of the delivery main pipe 23 are closed, and the fifth delivery pipe 43 is kept connected with the first delivery pipe 11. The medium enters the fifth delivery pipe 43 in reverse from the base of the nozzle. In this process, the pipe diameter design scheme of the above-mentioned first diversion pipe 61 and the second diversion pipe 63 can ensure the balanced suction force at the nozzle end to avoid the problem of suction blockage.

[0027] A flow guide 62 is provided at the junction of the first diverter pipe 61 and the first delivery pipe 11. This flow guide 62 comprises at least two coaxial flow guide tubes 623 arranged inside and outside, connected by a connecting plate 622. A first filler 621 is placed between the first diverter pipe 61 and the flow guide 622. First fillers 621 are arranged at intervals around the outer wall of the flow guide tubes 623 adjacent to the inner wall of the first diverter pipe 61. The internal and external distribution of the flow guide tubes 623 and the connecting plate 622 transforms the disordered inlet flow into a stable, symmetrical distribution. By guiding the fluid step by step through the multi-stage cylindrical sleeves, the flow gradient is smoothly transitioned, reducing turbulent kinetic energy and suppressing vortex formation, thereby improving spray uniformity. Furthermore, the internal and external distribution of the flow guide tubes 623 and the connecting plate 622 enhance the shear effect of the fluid by changing the cross-sectional area and direction of the flow path. This promotes uniform dispersion of particles or bubbles when transporting mixed fluids, such as when delivering nutrient solution or water-gas mixtures into water. In addition, the arrangement of the flow guide 62 can reduce blockage at the connection between the first diversion pipe 61 and the first delivery pipe 11, and the high shear force achieved by the flow guide 62 can make the fluid flush the pipe wall and inhibit dirt adhesion.

[0028] A stirring assembly 70 is built into the culture chamber. The stirring assembly 70 includes a stirring shaft 75 with spiral blades on the surface. Stirring blades 73 are arranged spirally around the outer side of the stirring shaft 75. The stirring blades 73 are connected to the stirring shaft 75 through a stirring rod 72. An auxiliary plate 74 is provided at the end of the stirring shaft 75, and the auxiliary plate 74 is connected to the stirring rod 72. One end of the auxiliary plate 74 is connected to the stirring shaft 75, and the other end is connected to the stirring shaft 75. The cooperation between the stirring shaft 75, the stirring blades 73 and the stirring rod 72 can achieve stirring and breaking up soil clumps, so that organic matter, microorganisms and added nutrients are evenly distributed, avoiding local nutrient excess or shortage. At the same time, the auxiliary plate 74 set at the end of the stirring shaft 75 can expand the stirring range and continuously exchange the materials at its end during the stirring process to improve the material stirring and movement effect, thereby avoiding the problem of heat accumulation in local areas and preventing microbial inactivation.

[0029] A driving motor connected to the stirring shaft 75 of the stirring assembly 70 is built into the collecting chamber to realize the rotational movement of the stirring assembly 70 .

[0030] The partition plate 13 is a perforated plate, specifically a mesh or grid-shaped partition plate that can support the soil and facilitate the discharge of excess water, media, etc. to simulate the displacement of water and gas in the soil in a natural environment.

[0031] A feed port is provided at the upper end of the first tank body 10, which is used to feed materials such as soil, microplastics, nutrient solution, etc. At the same time, monitoring equipment such as sensors, CCD cameras, etc. can be taken into the first tank body 10 through the feed port. A discharge pipe 12 is provided at the lower end of the first tank body 10 for discharging the medium.

[0032] Example 2: See the instructions attached Figure 7 , Attachment Figure 8 As shown, the first tank body 10 in a soil microplastic surface microorganism cultivation device of the present invention has at least two, in this embodiment, three first tank bodies 10, each first tank body 10 is equipped with a fourth tank body 40 and a third tank body 30 corresponding to its number, that is, each first tank body 10 is connected to a fourth tank body 40 and a third tank body 30, in this embodiment, a second tank body 20 is equipped, and the second tank body 20 is connected to the main conveying pipe 23 through a second conveying pipe 21. This solution can reduce the number of second tank bodies 20 used, reduce the site space and the cost of the entire production line layout.

[0033] The above-mentioned layout method can realize batch and large-scale microbial cultivation, and by controlling the medium conveyed and the culture conditions of each first tank 10, microbial cultivation with different condition parameters can be carried out in the same batch, thereby shortening the microbial cultivation time and obtaining microorganisms cultured under different conditions.

[0034] An auxiliary pump body 22 is provided on one side of the second tank body 20 and is connected to it. The auxiliary pump body 22 is a booster pump. A water pump connected to the second delivery pipe 21 is provided above the second tank body 20. The water in the second tank body 20 is pumped by the water pump in the second tank body 20 to the second delivery pipe 21. The auxiliary pump body 22 can increase the water pressure, so that the water pressure of the extracted and discharged fluid is increased to achieve long-distance delivery to each first tank body 10, solving the problem that the delivery flow rate of each first tank body 10 is different due to the different delivery lengths of the delivery medium.

[0035] It should also be noted that the terms "center", "up", "down", "left", "right", "vertical", "horizontal", "inside", "outside", etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on this application. Unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", etc. should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or an indirect connection through an intermediate medium, or it can be a communication between the internal parts of two elements. For those of ordinary skill in the art, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0036] The embodiments and / or implementation methods described above are only used to illustrate the preferred embodiments and / or implementation methods for realizing the technology of the present invention, and do not impose any form of limitation on the implementation methods of the technology of the present invention. Any person skilled in the art may make slight changes or modifications to other equivalent embodiments without departing from the scope of the technical means disclosed in the content of the present invention, but they should still be regarded as technologies or embodiments that are essentially the same as the present invention.

[0037] This article uses specific examples to illustrate the principles and implementation methods of this application. The description of the above embodiments is only used to help understand the method and core ideas of this application. The above is only the preferred implementation method of this application. It should be pointed out that due to the limitations of textual expression, there are objectively infinite specific structures. For ordinary technicians in this technical field, without departing from the principles of this application, they can also make several improvements, modifications or changes, and can also combine the above technical features in an appropriate manner; these improvements, modifications, changes or combinations, or the direct application of the inventive concept and technical solution to other occasions without improvement, should be regarded as the scope of protection of this application.

Claims

1. A soil microplastic surface microorganism cultivation device, comprising: a first tank body (10); a third tank body (30) and a fourth tank body (40) connected to the first tank body (10) are provided on one side of the first tank body (10); a main delivery pipe (23) is provided above the first tank body (10); the first tank body (10) and the main delivery pipe (23) are connected via a first delivery pipe (11); and the device is characterized in that: The third tank body (30) is connected to the first tank body (10) through the seventh delivery pipe (32), and the third tank body (30) is connected to the delivery main pipe (23) through the third delivery pipe (31). The bottom of the fourth tank body (40) is connected to the first pump body (50), and the first pump body (50) is connected to the seventh delivery pipe (32) through the sixth delivery pipe (51).

2. A soil microplastic surface microorganism cultivation device according to claim 1, characterized in that: The fourth tank body (40) is in communication with the first tank body (10) via a fourth delivery pipe (41), and the fourth tank body (40) is in communication with the delivery main pipe (23) via a fifth delivery pipe (42).

3. The soil microplastic surface microorganism cultivation device according to claim 1, characterized in that: A second tank body (20) is provided outside the first tank body (10), and the second tank body (20) is connected to the main delivery pipe (23) via a second delivery pipe (21).

4. The soil microplastic surface microorganism cultivation device according to claim 1, characterized in that: The first tank body (10) has a built-in partition plate (13), and the partition plate (13) divides the first tank body (10) into a culture chamber and a collection chamber arranged above and below. There are at least two seventh delivery pipes (32), and the third tank body (30) is connected to the culture chamber and the collection chamber respectively through the seventh delivery pipes (32).

5. The soil microplastic surface microorganism cultivation device according to claim 4, characterized in that: A medium distribution assembly (60) connected to the first delivery pipe (11) is provided above the culture chamber. The medium distribution assembly (60) includes a first shunt pipe (61) connected to the output end of the first delivery pipe (11). Second shunt pipes (63) are arranged in an array on the side of the first shunt pipe (61). The output port of the second shunt pipe (63) is provided with a nozzle.

6. The soil microplastic surface microorganism cultivation device according to claim 5, characterized in that: A flow guide (62) is provided at the connection between the first diverter pipe (61) and the first delivery pipe (11), the flow guide (62) comprising at least two coaxial flow guide pipes (623) arranged inside and outside, the flow guide pipes (623) being connected via a connecting plate (622), and a first filling member (621) being filled between the first diverter pipe (61) and the flow guide (62).

7. The soil microplastic surface microorganism cultivation device according to claim 4, characterized in that: The culture chamber is provided with a stirring assembly (70), the stirring assembly (70) comprising a stirring shaft (75) with spiral blades on the surface, stirring blades (73) arranged spirally around the outer side of the stirring shaft (75), the stirring blades (73) being connected to the stirring shaft (75) via a stirring rod (72), an auxiliary plate (74) being provided at the end of the stirring shaft (75), and the auxiliary plate (74) being connected to the stirring rod (72).

8. The soil microplastic surface microorganism cultivation device according to claim 7, characterized in that: A driving motor connected to the stirring shaft (75) of the stirring assembly (70) is built into the collecting chamber.

9. The soil microplastic surface microorganism cultivation device according to claim 4, characterized in that: The partition plate (13) is a perforated plate.

10. The soil microplastic surface microorganism cultivation device according to claim 1, characterized in that: A feed port is provided at the upper end of the first tank body (10), and a discharge pipe (12) is provided at the lower end of the first tank body (10).

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