Cell liquid-changing culture dish based on inclination control, culture system and culture method thereof

Abstract

The invention provides a cell liquid changing culture dish based on inclination control, a culture system and a culture method, and belongs to the technical field of bioengineering. The cell liquid changing culture dish comprises a liquid storage cabin, a culture cabin and a waste liquid cabin; a liquid storage cabin, a culture cabin and a waste liquid cabin are designed in a layered manner, a first liquid guide port and a second liquid guide port which are inclined in a first direction to form high and low positions are formed in the liquid storage cabin, and flushing liquid and culture liquid in a first liquid storage chamber and a second liquid storage chamber are independently guided into a culture chamber through different inclination angles in the first direction; a third liquid guide port which inclines based on the second direction in the culture cabin and is mutually higher and lower than the first liquid guide port and the second liquid guide port is used for guiding the liquid in the culture chamber into the waste liquid chamber through inclination in the second direction; the whole structure is free of pipeline design, manual operation can be achieved, and timed and quantitative liquid changing and adding can be achieved by controlling the inclination direction and angle through automatic equipment.

Description

Technical Field

[0001] The invention belongs to the field of biotechnology engineering, and in particular relates to a cell fluid exchange culture dish based on tilt control, a culture system and a culture method thereof. Background Art

[0002] Current cell culture fluid exchange technologies are primarily categorized as manual and automated. Manual operation remains the predominant method in laboratories, using manual pipettes or vacuum pumps to remove waste fluid and add new culture medium. Automated equipment, which has emerged in recent years, often utilizes pumps, valves, and piping systems to transfer fluids.

[0003] However, the above-mentioned existing technologies require a lot of manpower for manual operation, which greatly affects the culture results due to the lack of professional skills of the personnel. The interval between liquid changes cannot be accurately controlled, frequent opening of the lid easily leads to contamination, and delay control is impossible. Automated equipment requires external pumps, valves, and pipelines, which are complex and costly, and residual liquid can easily breed bacteria. Therefore, the present invention has been developed.

[0004] It should be noted that the information disclosed in the background technology section of the invention is only intended to deepen the understanding of the general background technology of the invention, and should not be regarded as an admission or any form of implication that the information constitutes prior art already known to those skilled in the art. Summary of the Invention

[0005] One object of the present invention is to provide a culture dish that can be rinsed and replaced by tilting without opening the lid. The second object of the present invention is to provide a culture system that can automatically control flushing and liquid replacement by tilting without opening the cover.

[0006] The third object of the present invention is to provide a culture method with automatic control of flushing and liquid replacement.

[0007] To solve one of the above purposes, the present invention first provides a cell culture dish for changing fluid based on tilt control, comprising: A liquid storage tank, wherein the liquid storage tank has a first liquid storage chamber and a second liquid storage chamber that are independent of each other, the first liquid storage chamber is provided with a first liquid guide port and a first liquid filling port, the second liquid storage chamber is provided with a second liquid guide port and a second liquid filling port, the first liquid guide port and the second liquid guide port are inclined based on a first direction to form a high point and a low point relative to each other; a culture chamber located below the liquid storage chamber and having a culture chamber connected to the first liquid guide port and the second liquid guide port, the culture chamber being provided with a third liquid guide port and a third liquid addition port, the third liquid guide port being inclined relative to the first liquid guide port and the second liquid guide port in a second direction, the second direction being different from the first direction; The waste liquid tank is located below the culture tank and has a waste liquid chamber connected to the third liquid guide port; the waste liquid chamber has a waste discharge port in the same direction as the first liquid addition port and the second liquid addition port.

[0008] Preferably, the first liquid storage chamber and the second liquid storage chamber are formed by dividing the liquid storage tank to the left and right by a partition; the first liquid guide port and the second liquid guide port are respectively located on the side walls of the liquid storage tank away from the partition, and are respectively away from the first liquid filling port and the second liquid filling port.

[0009] Preferably, a first guide slope is provided on the side wall of the first liquid storage chamber relatively away from the partition, and the first liquid guide port is located at the top of the first guide slope; the liquid storage level of the first liquid storage chamber is lower than the first liquid guide port when in a horizontal position.

[0010] Preferably, a second guide slope is provided on the side wall of the second liquid storage chamber relatively away from the partition, and the second liquid guide port is located at the top of the second guide slope; the liquid storage level of the second liquid storage chamber is lower than the second liquid guide port when in a horizontal position.

[0011] Preferably, the first liquid storage chamber and the second liquid storage chamber are formed by dividing the liquid storage compartment into left and right parts by a partition; the first liquid guide port and the second liquid guide port are arranged on two sides of the partition that are away from each other.

[0012] Preferably, the structures of the first liquid storage chamber and the second liquid storage chamber are L-shaped; the second liquid storage chamber has a bridging section, which is erected above the first liquid storage chamber, and a bridge space is formed below the bridging section, so that the bridging sections of the first liquid storage chamber and the second liquid storage chamber are arranged in layers; the first liquid guide port and the second liquid guide port are respectively located at the ends of the L-shaped structures of the first liquid storage chamber and the second liquid storage chamber.

[0013] Preferably, the culture chamber and the liquid storage chamber are staggered and stacked, the culture chamber partially protrudes from the liquid storage chamber and forms an observation window; the culture chamber has a third liquid filling port close to or located at the observation window.

[0014] Preferably, the third liquid guide port is provided with a third guide slope, and the third liquid guide port is located at the central top of the third guide slope; the third liquid adding port and the third liquid guide port are far away from each other; the liquid level of the culture chamber is lower than the third liquid guide port when in a horizontal position.

[0015] The technical effects produced by the above technical solutions of the present invention come from one or more of the following combinations: By designing the liquid storage tank, culture tank and waste liquid tank in layers, a first liquid guide port and a second liquid guide port are provided in the liquid storage tank at high and low positions based on an inclination in a first direction, and the flushing liquid and the culture liquid in the first liquid storage chamber and the second liquid storage chamber are respectively and independently introduced into the culture chamber in the lower layer at different inclination angles in the first direction; a third liquid guide port is provided in the culture tank at a high and low position relative to the first liquid guide port and the second liquid guide port based on an inclination in a second direction, and the liquid in the culture chamber is introduced into the waste liquid chamber by inclination in the second direction; the overall structure has no pipeline design and can be operated manually, or the inclination direction and angle can be controlled by automated equipment to achieve timed and quantitative liquid replacement and addition.

[0016] The first liquid storage chamber and the second liquid storage chamber are independently separated by a partition. Even if tilt control is performed in the first direction, the first liquid guide port and the second liquid guide port will not form mixed liquids due to the difference in high and low positions. Similarly, when tilt control is performed in the second direction, tilt control is only performed in one direction to ensure that the first liquid guide port and the second liquid guide port are always at a high position relative to the third liquid guide port of the culture chamber, thereby ensuring that when the liquid in the culture chamber is discharged, no flushing liquid and culture liquid will flow into the culture chamber from the liquid storage chamber.

[0017] The culture chamber and the liquid storage chamber are staggered and stacked, forming a third liquid addition port for convenient cell inoculation and extraction operations and an observation window for convenient observation, so as to realize visual operation. The upper surface of the bottom of the culture chamber should be hydrophilic to facilitate cell adhesion and growth.

[0018] Guide slopes are provided at the first liquid guide port, the second liquid guide port and the third liquid guide port, respectively, to facilitate better guiding of the liquid flow and to facilitate the complete outlet of all liquids with a minimum inclination angle.

[0019] To solve the above-mentioned second purpose, the present invention provides a cell fluid replacement culture system based on tilt control, comprising a tilt control console and a cell fluid replacement culture dish based on tilt control; the cell fluid replacement culture dish is placed on the tilt control console and fixed.

[0020] Preferably, the tilting console includes a tray and a universal automatic tilting support.

[0021] Preferably, the universal automatic tilt support comprises: A support rod, one end of which is supported on the bottom of the tray through a universal joint, and the other end is fixed to the center of the base; Multiple telescopic adjustment rods, one end of each of the telescopic adjustment rods is supported on the bottom of the tray and located at the corner of the tray through the universal joint, and the other end is supported on the support through the universal joint, and the multiple telescopic adjustment rods are surrounded by the support rod; A telescopic control member is connected to the plurality of telescopic adjustment rods.

[0022] The technical effects produced by the above technical solutions of the present invention come from one or more of the following combinations: Simply place the cell culture dish on the tray of the tilting console and secure it. By automatically controlling the length of the multiple telescopic adjustment rods of the universal automatic tilting support, the tray can be tilted in a specific direction and at a certain angle to perform fluid replacement and addition operations. Additional IoT, Bluetooth and other modules support remote setting of the fluid replacement program and viewing of the fluid replacement progress, and real-time recording is carried out to facilitate the formation of culture reports.

[0023] To achieve the above three purposes, the present invention provides a culture method of a cell culture system based on tilt control, which implements at least one of the following steps: A first direction of the tilting console is achieved by tilting the console, and when the first liquid guide port is at a low position, the flushing liquid is introduced into the culture chamber for flushing; The tilting console is used to achieve a high and low tilt in the first direction, and when the second liquid guide port is at a low position, the culture liquid is introduced into the culture chamber to achieve culture; The height tilt in the second direction is achieved by the tilting console, and the liquid in the culture chamber is introduced into the waste liquid chamber when the third liquid guide port is at a low position.

[0024] The technical effects produced by the above technical solutions of the present invention come from one or more of the following combinations: Simply place the cell culture dish on the tray of the tilting console and secure it. By automatically controlling the length of the multiple telescopic adjustment rods of the universal automatic tilting support, the tray can be tilted in a specific direction and at a certain angle, thus enabling fluid replacement and addition operations. This is simple, convenient, and pollution-free throughout the process. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 The schematic diagram of the structure of the cell medium replacement culture dish of the present invention is expressed.

[0026] Figure 2 The exploded view of the structure of the liquid storage tank, culture tank and waste liquid tank in the cell fluid exchange culture dish of the present invention is expressed.

[0027] Figure 3 A schematic diagram of the internal structure of the cell medium exchange culture dish of the present invention is expressed.

[0028] Figure 4 The tilting operation in the cell medium replacement culture dish of the present invention is expressed with reference to FIG.

[0029] Figure 5 Another position diagram of the first liquid guide port and the second liquid guide port in the cell fluid exchange culture dish of the present invention is expressed.

[0030] Figure 6Another position diagram of the first and second liquid guide ports in the cell culture dish of the present invention is shown Figure 7 expressed Figure 6 Stereoscopic perspective diagram.

[0031] Figure 8 The schematic diagram of the structure of the cell culture system for fluid replacement of the present invention is expressed.

[0032] Among them: 1. Cell exchange culture dish; 11. Liquid storage tank; 111. First liquid storage chamber; 1110. First liquid adding port; 1111. First liquid guide port; 1112. Bridge space; 112. Second liquid storage chamber; 1120. Second liquid adding port; 1121. Second liquid guide port; 1122. Bridge section; 113. First guide slope; 114. Partition; 12. Culture chamber; 120. Culture chamber; 121. Third liquid adding port; 122. Observation window; 123. Third liquid guide port; 124. Third guide slope; 13. Waste liquid chamber; 131. Waste outlet; 21. Tray; 221. Support rod; 222. Telescopic adjustment rod; 223. Universal joint. DETAILED DESCRIPTION

[0033] The following description is provided to enable those skilled in the art to make and use the present invention and to incorporate it into a specific application context. Various modifications and uses in different applications will be readily apparent to those skilled in the art, and the general principles defined herein are applicable to a wide range of embodiments. Thus, the present invention is not limited to the embodiments set forth herein, but should be accorded the broadest scope consistent with the principles and novel features disclosed herein.

[0034] In the following detailed description, many specific details are set forth to provide a more thorough understanding of the present invention. However, it will be apparent to those skilled in the art that the practice of the present invention need not be limited to these specific details. In other words, known structures and devices are shown in block diagram form without detailed display to avoid obscuring the present invention.

[0035] The reader's attention is drawn to all documents and materials filed concurrently with this specification and open to public inspection, and the contents of all such documents and materials are incorporated herein by reference. Unless otherwise expressly stated, all features disclosed in this specification (including any accompanying claims, abstracts, and drawings) may be replaced by alternative features serving the same, equivalent, or similar purpose. Therefore, unless expressly stated otherwise, each feature disclosed is merely an example of a group of equivalent or similar features.

[0036] Note that where used, the terms left, right, front, back, top, bottom, forward, reverse, clockwise, and counterclockwise are used for convenience only and do not imply any specific fixed direction. Instead, they are used to reflect the relative position and / or orientation of various parts of an object. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0037] In the description of the present invention, it should be noted that, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "connected" should be understood broadly. For example, they may refer to fixed, detachable, or integral connections; mechanical connections; direct connections or indirect connections through an intermediary; and internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in the present invention based on specific circumstances.

[0038] Note that, where used, "further," "preferably," "further," and "more preferably" are simply prefaces for describing another embodiment based on the preceding embodiment. The contents of the "further," "preferably," "further," or "more preferably" combined with the preceding embodiment constitute a complete configuration of another embodiment. Multiple "further," "preferably," "more preferably," or "more preferably" clauses appended to the same embodiment can be arbitrarily combined to form yet another embodiment.

[0039] The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments. Note that the various aspects described below with reference to the accompanying drawings and specific embodiments are merely exemplary and should not be construed as limiting the scope of protection of the present invention.

[0040] Example 1: See also Figures 1 to 5This embodiment provides a cell fluid exchange culture dish 1 based on tilt control, including a liquid storage chamber 11, a culture chamber 12 and a waste liquid chamber 13. Among them, the liquid storage tank 11 has a first liquid storage chamber 111 and a second liquid storage chamber 112 that are independent of each other, the first liquid storage chamber 111 is provided with a first liquid guide port 1111, and the second liquid storage chamber 112 is provided with a second liquid guide port 1121, and the first liquid guide port 1111 and the second liquid guide port 1121 are inclined to each other at a high point and a low point based on a first direction; the culture chamber 12 is located below the liquid storage tank 11 and has a culture chamber 120 connected to the first liquid guide port 1111 and the second liquid guide port 1121, and the culture chamber 120 is provided with a third liquid guide port 123, and the third liquid guide port 123 and the first liquid guide port 1111 and the second liquid guide port 1121 are inclined to each other at a high point and a low point based on a second direction; the second direction is different from the first direction; the waste liquid tank 13 is located below the culture tank 12 and has a waste liquid chamber connected to the third liquid guide port 123; the waste liquid chamber has a waste outlet 131 in the same direction as the first liquid adding port 1110 and the second liquid adding port 1120.

[0041] In this embodiment, the purpose of the first direction is to achieve high and low positions by tilting the first liquid guide port 1111 and the second liquid guide port 1121 at opposite angles; the purpose of the second direction is to achieve high and low positions by tilting the third liquid guide port 123 and the first liquid guide port 1111 and the second liquid guide port 1121 at opposite angles.

[0042] As a preferred implementation of this embodiment, please refer to Figure 4 The first direction is the Y direction and the second direction is the X direction. The embodiment is described in detail by tilting left and right to achieve the opposite angles of the first direction and the second direction.

[0043] In this embodiment, the liquid storage chamber 11, the culture chamber 12 and the waste liquid chamber 13 are preferably box-shaped and designed in a stacked manner. The stacking method can be integrally formed by 3D printing or designed in a layered manner with layer-by-layer connection.

[0044] like Figure 2As shown, the first liquid storage chamber 111 and the second liquid storage chamber 112 in the liquid storage tank 11 are separated into left and right by the partition 114, and the first liquid guide port 1111 and the second liquid guide port 1121 are respectively located on the side walls of the liquid storage tank 11 away from the partition 114. Therefore, when tilted to the left in the Y direction, the second liquid guide port 1121 is located at the highest point and the first liquid guide port 1111 is located at the lowest point, so that the liquid overflows from the first liquid guide port 1111 into the culture chamber 12. At this time, the first liquid storage chamber 111 can store the pre-flushing flushing liquid; similarly, when tilted to the right in the Y direction, the first liquid guide port 1111 is located at the highest point and the second liquid guide port 1121 is located at the lowest point, so that the liquid overflows from the second liquid guide port 1121 into the culture chamber 12. At this time, the second liquid storage chamber 112 can store the culture liquid for liquid addition culture.

[0045] Furthermore, a first guide slope 113 is provided on the sidewall of the first liquid storage chamber 111, relatively far from the partition 114, and a first liquid guide port 1111 is located at the top of the first guide slope 113. When the liquid level in the first liquid storage chamber 111 is horizontally lower than the first liquid guide port 1111. Similarly, a second guide slope is provided on the sidewall of the second liquid storage chamber 112, relatively far from the partition 114, and a second liquid guide port 1121 is located at the top of the second guide slope. When the liquid level in the second liquid storage chamber 112 is horizontally lower than the second liquid guide port 1121.

[0046] Furthermore, a first liquid adding port 1110 and a second liquid adding port 1120 are provided at one end of the first liquid storage chamber 111 and the second liquid storage chamber 112; and correspondingly, the first liquid guide port 1111 and the second liquid guide port 1121 are provided at the other end, and the lowest point of the openings of the first liquid adding port 1110 and the second liquid guiding port 1120 should be slightly higher than the first liquid guide port 1111 and the second liquid guide port 1121 to prevent overflow during liquid addition.

[0047] As shown in the figure, the first liquid guide port 1111 is located at the top of the first guide slope 113 and at the end position of the first liquid storage chamber 111, and the first liquid filling port 1110 is located at the head end position of the first liquid storage chamber 111. Furthermore, the first liquid guide port 1111 is located on the side away from the partition 114; similarly, the second liquid guide port 1121 is located at the top of the second guide slope and at the end position of the second liquid storage chamber 112, and the second liquid filling port 1120 is located at the head end position of the second liquid storage chamber 112. Furthermore, the second liquid guide port 1121 is located on the side away from the partition 114.

[0048] In this embodiment, Figure 3 As shown, the culture chamber 12 and the liquid storage chamber 11 are staggered and stacked, and the culture chamber 12 partially protrudes from the liquid storage chamber 11 to form an observation window 122; the culture chamber 12 has a third liquid filling port 121 near or located at the observation window 122.

[0049] Furthermore, a third guide slope 124 is provided at the third liquid guide port 123. The third liquid guide port 123 is located at the central top of the third guide slope 124, and the third liquid guide port 123 is positioned at the other end of the first liquid guide port 1111 and the second liquid guide port 1121. Thus, when the third liquid guide port 123 is at a low point to discharge liquid, the first liquid guide port 1111 and the second liquid guide port 1121 are at high points, preventing liquid from flowing out. Therefore, when tilted to the right in the X direction, the first liquid guide port 1111 and the second liquid guide port 1121 are at their highest points, the liquid levels in the first liquid storage chamber 111 and the second liquid storage chamber 112 are lower than those in the first liquid guide port 1111 and the second liquid guide port 1121, and the third liquid guide port 123 is at its lowest point, causing liquid to overflow from the third liquid guide port 123 into the waste liquid chamber 13.

[0050] As another implementation of this embodiment, please refer to Figure 5 The first liquid inlet 1111 and the second liquid inlet 1121 can also be provided on the partition 114, specifically on opposite sides of the partition 114. In this case, the partition 114 is composed of multiple plates forming a cavity that is internally connected to the culture chamber. This cavity serves as a transition space, allowing the first liquid storage chamber 111 to communicate with the culture chamber through the first liquid inlet 1111; and / or allowing the second liquid storage chamber 112 to communicate with the culture chamber through the second liquid inlet 1121.

[0051] As another implementation of this embodiment, please refer to Figure 6 and Figure 7 In this embodiment, the first liquid storage chamber 111 and the second liquid storage chamber 112 have an L-shaped structure. Specifically, Figure 6 The arrows in the figure show the liquid flow paths of the two liquid storage chambers. Figure 7 The second liquid storage chamber 112 has a bridging section 1122, which is erected above the first liquid storage chamber 111, and a bridge space 1112 is formed below the bridging section 1122, so that the bridging sections 1122 of the first liquid storage chamber 111 and the second liquid storage chamber 112 are arranged in layers. The first liquid guide port 1111 and the second liquid guide port 1121 are respectively located above the ends of the L-shaped structures of the first liquid storage chamber 111 and the second liquid storage chamber 112 and are respectively connected to the culture chamber. The first liquid guide port 1111 and the second liquid guide port 1121 of this embodiment also meet the requirements of the high and low positions of the inclined setting, and can achieve the effect of independent liquid conduction. Preferably, since the second liquid guide port 1121 passes through the bridge space 1112, the second liquid guide port 1121 can be implemented as a channel port structure.

[0052] It should be noted that this embodiment provides three position structures of the first liquid guide port 1111 and the second liquid guide port 1121. However, the position structures of the two should not be limited thereto.

[0053] Preferably, the first liquid addition port 1110, the second liquid addition port 1120, the third liquid addition port 121 and the waste outlet 131 are circular, upwardly inclined, threaded bottle openings, and are equipped with threaded bottle caps with sealing rings. If all the threaded bottle caps are tightened, the entire cell exchange culture dish should be in a sealed state.

[0054] Furthermore, the first liquid addition port 1110 and the second liquid addition port 1120 are opened only when adding liquid, the third liquid addition port 121 is opened when cells are first inoculated, the bottle cap is loosened when cells are cultured to achieve a certain amount of internal and external gas exchange, and the waste outlet 131 is opened only when waste liquid is dumped.

[0055] Preferably, the entire cell culture dish chamber is made of transparent plastic material, and the upper surface of the bottom of the cell culture chamber is hydrophilized to facilitate cell growth.

[0056] Beneficial effects of this embodiment: By designing the liquid storage tank 11, the culture tank 12 and the waste liquid tank 13 in layers, the first liquid guide port 1111 and the second liquid guide port 1121 are formed in the liquid storage tank 11 at different heights based on the inclination in the first direction, and the flushing liquid and the culture liquid in the first liquid storage chamber 111 and the second liquid storage chamber 112 are independently introduced into the culture tank 12 in the lower layer at different inclination angles in the first direction; in the culture tank 12, the third liquid guide port 123 is formed in the culture tank 12 at a higher height than the first liquid guide port 1111 and the second liquid guide port 1121 based on the inclination in the second direction, and the liquid in the culture chamber is introduced into the waste liquid chamber by inclination in the second direction; the overall structure has no pipeline design, can be manually operated, and can also be freely controlled by angle inclination through automated equipment, thereby reducing the risk of contamination, and realizing directional flow of liquid only by utilizing the principles of gravity and fluid guidance.

[0057] The first liquid storage chamber 111 and the second liquid storage chamber 112 are independently separated by the partition 114. Even if the tilt control is performed in the first direction, the first liquid guide port 1111 and the second liquid guide port 1121 will not form mixed liquid due to the difference in high and low positions; similarly, the first liquid guide port 1111 and the second liquid guide port 1121 are simultaneously tilt-controlled in the second direction together with the third liquid guide port 123 of the culture chamber 12. When the third liquid guide port 123 is in a low position, the difference in high and low positions will not cause the flushing liquid and the culture liquid to flow from the liquid storage chamber 11 into the culture chamber 12.

[0058] The culture chamber 12 and the liquid storage chamber 11 are staggered and stacked to form a third liquid adding port 121 for easy operation and an observation window 122 for easy observation, thereby realizing visual operation and facilitating microscopic observation.

[0059] Guide slopes are provided at the first liquid guiding port 1111 , the second liquid guiding port 1121 and the third liquid guiding port 123 respectively to facilitate better guiding of liquid flow.

[0060] It should be noted that the present invention aims to complete aseptic operations through tilt control, realize aseptic culture of liquid replacement and liquid addition with a simple structure, reduce the possibility of cell contamination, improve the simplicity of operation, and reduce the high technical dependence on the operator.

[0061] Example 2: See also Figure 6 Combined with Figures 1 to 5 This embodiment provides a tilt-controlled cell culture dish 1 molding system, comprising a tilt control console and a tilt-controlled cell culture dish 1; the cell culture dish 1 is placed on the tilt control console. Furthermore, the bottom of the cell culture dish 1 should be fixed to the tilt control console. This can be achieved by providing a fixing groove on the tilt control console that fits the bottom of the cell culture dish 1, or by securing it with elastic bands, adhesive, or other methods. This is to prevent the cell culture dish 1 from shifting or becoming unstable, which could cause a deviation in the tilt angle.

[0062] In this embodiment, the cell medium replacement culture dish 1 in embodiment 1 is automatically operated through the automatic control of the tilting console, thereby eliminating the problems of imprecise and inconvenient manual operation.

[0063] Specifically, the tilt control console includes a tray 21 and a universal automatic tilt support. Furthermore, the universal automatic tilt support includes a universal joint 223, a support rod 221, multiple telescopic adjustment rods 222, and a telescopic control member. The universal joint 223 is a sphere connected to a long rod installed in a spherical chamber. The spherical chamber covers more than half of the sphere's area, preventing the sphere from leaving the chamber and allowing it to rotate within a certain angle. The diameter of the connected long rod is much smaller than the diameter of the sphere, and the long rod can rotate with the rotation of the sphere. One end of the support rod 221 is supported by the universal joint 223 at the bottom of the tray 21, and the other end is fixed to the center of the support. One end of the telescopic adjustment rod 222 is supported by the universal joint 223 at the bottom of the tray 21 and is located at the corner of the tray 21. The other end is supported by the universal joint 223 on the support. Multiple telescopic adjustment rods 222 are arranged around the support rod 221. The telescopic control member controls the connection to the multiple telescopic adjustment rods 222.

[0064] The telescopic control unit includes a motor, a power supply, and a control module. The control module controls the motor to adjust the telescopic adjustment rods 222. The telescopic adjustment rods 222 can be implemented as hydraulic rods, screw rods, rack and pinion mechanisms, electric push rods, etc., to control the length of the telescopic adjustment rods and drive the tray 21 to tilt in multiple directions and angles within a set angle range.

[0065] The control module is a single-chip microcomputer or microprocessor with a built-in preset program. This program controls the direction and volume of liquid flow by controlling the telescopic control element to achieve a set tilt time, tilt direction, and tilt angle. An event log is also set to record adjustment data and generate a culture experiment report. The control module can also provide feedback to the client via Bluetooth and IoT modules, receiving user instructions and reporting device status.

[0066] It should be noted that the present invention aims to complete aseptic operation by tilting the device, realize aseptic culture by changing and adding liquid with a simple structure, reduce the possibility of cell contamination, improve the simplicity of operation, and reduce the high technical dependence of the operator. The beneficial effects of this embodiment are: Simply place the cell culture dish 1 on the tray 21 of the tilting console and fix it, initially in a horizontal position. By automatically controlling the length of multiple telescopic adjustment rods of the universal automatic tilting support, the tray can be tilted in a specific direction and at a certain angle, thereby achieving multiple fluid changes and additions. Additional IoT, Bluetooth and other modules support remote setting of fluid change programs and viewing of fluid change progress, allowing for real-time recording and convenient formation of culture reports.

[0067] Example 3: Please combine Figures 1 to 6 This embodiment provides a culture method for a cell culture system based on tilt control, which implements at least one of the following steps: The flushing solution, the culture solution and the cells to be cultured are added through the first liquid adding port 1110 , the second liquid adding port 1120 and the third liquid adding port 121 respectively.

[0068] Specifically, the tilting console is initially in a horizontal position, flushing liquid is added to the first liquid storage chamber 111 through the first liquid adding port 1110, culture fluid is added to the second liquid storage chamber 112 through the second liquid adding port 1120, and cells to be cultured and initial culture fluid are added to the culture chamber 120 through the third liquid adding port 121. After a certain period of culture under certain temperature, humidity, carbon dioxide concentration and sterile conditions, the cells achieve adherent growth.

[0069] The height tilt in the first direction is achieved by tilting the console, and when the first liquid guide port 1111 is at a low position, the flushing liquid is introduced into the culture chamber 120 for flushing.

[0070] Specifically, the user can set the telescopic control member to control the telescopic adjustment rod 222 to adjust the tilt. The tray 21 is tilted in the Y direction, so that the first liquid guide port 1111 in the first liquid storage chamber 111 is at a low position. At this time, the first liquid storage chamber 111 is filled with flushing liquid, which enters the culture chamber 12 through the first liquid guide port 1111. After the flushing liquid is added, the tray 21 returns to a horizontal position.

[0071] The height tilt in the first direction is achieved by tilting the control console, and when the second liquid guide port 1121 is at a low position, the culture liquid is introduced into the culture chamber 120 to achieve culture.

[0072] Specifically, the user can set the telescopic control member to control the telescopic adjustment rod 222 to adjust the tilt. The tray 21 is tilted in the Y direction, so that the second liquid inlet 1121 in the second liquid storage chamber 112 is at a low position. At this time, the second liquid storage chamber 112 is filled with culture fluid, and the culture fluid enters the culture chamber 12 through the second liquid inlet 1121. After the culture fluid is added, the tray 21 returns to a horizontal position.

[0073] The height tilt in the second direction is achieved by tilting the console, and when the third liquid guide port 123 is at a low position, the liquid in the culture chamber 120 is guided into the waste liquid chamber.

[0074] Specifically, the user configures the telescopic control member to control the telescopic adjustment rod 222 for tilt adjustment. The tray 21 is tilted in the X direction, so that the third liquid inlet 123 in the culture chamber 120 is at a low position. The liquid in the culture chamber 120 flows into the waste liquid chamber through the third liquid inlet 123. At this time, the cells being cultured, due to their adherent growth, will not flow with the liquid. Once all the liquid has flowed into the waste liquid chamber, the tray 21 is returned to a horizontal position.

[0075] Beneficial effects of this embodiment: It is only necessary to place the cell exchange culture dish 1 with the added liquid and the cells to be cultured on the tray 21 of the tilting console and fix it. By automatically controlling the universal automatic tilting support to achieve tilting in a specific direction and at a certain angle, the culture chamber 120 can be flushed, the culture medium can be added, and the waste liquid can be discharged. Therefore, operations such as discarding waste liquid, flushing non-adherent cells with flushing liquid, and adding fresh culture medium in the cell culture process can be realized. It is simple and convenient, and there is no pollution throughout the process.

[0076] Furthermore, the present invention has been described in detail above with reference to the embodiments of the accompanying drawings. A person skilled in the art can make various modifications to the present invention based on the above description. Therefore, certain details in the embodiments should not be construed as limiting the present invention, and the scope of protection of the present invention shall be determined by the scope defined in the appended claims.

Claims

1. A cell culture dish for changing liquid based on tilt control, characterized in that: include: A liquid storage tank, wherein the liquid storage tank has a first liquid storage chamber and a second liquid storage chamber that are independent of each other, the first liquid storage chamber is provided with a first liquid guide port and a first liquid filling port, the second liquid storage chamber is provided with a second liquid guide port and a second liquid filling port, the first liquid guide port and the second liquid guide port are inclined based on a first direction to form a high point and a low point relative to each other; a culture chamber located below the liquid storage chamber and having a culture chamber connected to the first liquid guide port and the second liquid guide port, the culture chamber being provided with a third liquid guide port and a third liquid addition port, the third liquid guide port being inclined relative to the first liquid guide port and the second liquid guide port in a second direction, the second direction being different from the first direction; The waste liquid tank is located below the culture tank and has a waste liquid chamber connected to the third liquid guide port, and the waste liquid chamber has a waste discharge port in the same direction as the first liquid addition port and the second liquid addition port.

2. The tilt-controlled cell culture dish according to claim 1, wherein: The first liquid storage chamber and the second liquid storage chamber are formed by dividing the liquid storage tank to the left and right by a partition; the first liquid guide port and the second liquid guide port are respectively located on the side walls of the liquid storage tank away from the partition, and are respectively away from the first liquid filling port and the second liquid filling port.

3. The tilt-controlled cell culture dish according to claim 2, wherein: A first guide slope is provided on the side wall of the first liquid storage chamber relatively away from the partition, and the first liquid guide port is located at the top of the first guide slope; the liquid level of the first liquid storage chamber is lower than the first liquid guide port when in a horizontal position.

4. The tilt-controlled cell culture dish according to claim 2, wherein: A second guide slope is provided on the side wall of the second liquid storage chamber relatively away from the partition, and the second liquid guide port is located at the top of the second guide slope; the liquid level of the second liquid storage chamber is lower than the second liquid guide port when in a horizontal position.

5. The tilt-controlled cell culture dish according to claim 1, wherein: The first liquid storage chamber and the second liquid storage chamber are formed by dividing the liquid storage compartment into left and right parts by a partition; the first liquid guide port and the second liquid guide port are arranged on two sides of the partition that are away from each other.

6. The tilt-controlled cell culture dish according to claim 1, wherein: The structures of the first liquid storage chamber and the second liquid storage chamber are L-shaped; the second liquid storage chamber has a bridging section, which is erected above the first liquid storage chamber, and a bridge space is formed below the bridging section, so that the bridging sections of the first liquid storage chamber and the second liquid storage chamber are arranged in layers; the first liquid guide port and the second liquid guide port are respectively located at the ends of the L-shaped structures of the first liquid storage chamber and the second liquid storage chamber.

7. The tilt-controlled cell culture dish according to claim 1, wherein: The culture chamber and the liquid storage chamber are staggered and stacked, and the culture chamber partially protrudes from the liquid storage chamber to form an observation window; the culture chamber has a third liquid filling port close to or located at the observation window.

8. The tilt-controlled cell culture dish according to claim 1, wherein: The third liquid guide port is provided with a third guide slope, and the third liquid guide port is located at the central top of the third guide slope; the third liquid adding port and the third liquid guide port are far away from each other; the liquid level of the culture chamber is lower than the third liquid guide port when based on the horizontal position.

9. A cell culture system for changing liquid based on tilt control, characterized in that: The device comprises a tilting control console and a cell culture dish for fluid replacement based on tilting control according to any one of claims 1 to 6; the cell culture dish for fluid replacement is placed on the tilting control console and fixed.

10. A culture method of a cell culture system based on tilt control for liquid replacement according to claim 9, characterized in that: Perform at least one of the following steps: A first direction of the tilting console is achieved by tilting the console, and when the first liquid guide port is at a low position, the flushing liquid is introduced into the culture chamber for flushing; The tilting console is used to achieve a high and low tilt in the first direction, and when the second liquid guide port is at a low position, the culture liquid is introduced into the culture chamber to achieve culture; The height tilt in the second direction is achieved by the tilting console, and the liquid in the culture chamber is introduced into the waste liquid chamber when the third liquid guide port is at a low position.

Images