Growth-promoting microbial agent of variovorax P6-4 and motherwort herb as well as preparation method and application of growth-promoting microbial agent

Abstract

The invention provides a strain of variovorax P6-4, which is classified and identified as Variovorax sp. And is preserved in the China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC NO: M2025958. The invention also provides a motherwort growth-promoting microbial inoculum which comprises the variovorax P6-4 and an LB liquid culture medium. The invention provides a preparation method and application of the motherwort growth-promoting microbial agent, and also provides a method for promoting the growth of motherwort seedlings. The invention has the following advantages: a strain of variovorax sp. P6-4 is screened from soil in Nu Mountain Mountain area of Anhui Province, and the growth-promoting microbial inoculum prepared from the variovorax sp. P6-4 is used for growth of leonurus seedlings, so that the leonurus seedlings can be promoted to adapt to the environment, the absorption of soil nutrients and moisture is promoted, the development and growth of the seedlings are accelerated, and the survival rate of the leonurus seedlings is increased. The problems that in existing motherwort planting, the seedling growth rate is low, and the seedling growth vigor is poor are solved.

Description

Technical Field

[0001] The present invention relates to the technical field of agricultural microorganisms, and in particular to a strain of Bacillus thuringiensis P6-4 and a motherwort growth-promoting agent, as well as a preparation method and application thereof. Background Art

[0002] Motherwort (Leonurus japonicus) is a plant of the Lamiaceae family known for its tender stems and leaves. It promotes blood circulation, regulates menstruation, and promotes diuresis and swelling. It is used to treat menstrual disorders, dysmenorrhea, amenorrhea, lochia retention, edema, oliguria, and edema caused by acute nephritis. It is a commonly used medicinal herb in clinical prescriptions and a major raw material for pharmaceutical companies. Among the traditional Chinese medicines currently sold on the market, over 500 varieties use Leonurus as a raw material, and its market demand is high. Therefore, large-scale cultivation of Leonurus is of great significance.

[0003] However, during the cultivation of motherwort, it was found that the seedlings often experienced slow growth rates and poor growth. Analysis showed that the reason for this is that the growing seedlings cannot quickly and effectively utilize substances in the soil environment, resulting in slowed growth and poor growth. The existence of these problems will greatly reduce the benefits of using motherwort as a raw material for pharmaceutical production, resulting in significant economic losses.

[0004] The symbiosis between plants and growth-promoting rhizosphere bacteria refers to a mutually beneficial relationship in which specific bacteria colonize the plant root system (rhizosphere), promoting plant growth through direct or indirect physiological and biochemical interactions, while the plant provides the bacteria with nutrients such as carbon sources. This symbiotic relationship is primarily manifested in the following ways: bacteria enhance plant nutrient absorption and stress resistance through mechanisms such as nitrogen fixation, phosphate solubilization, phytohormone production, siderophore synthesis, and induction of systemic resistance; while plant root secretions (such as organic acids and sugars) provide the bacteria with the energy and carbon sources they need to survive, forming a dynamically balanced microecosystem. This symbiotic system has important applications in sustainable agricultural development and ecological restoration.

[0005] Based on this, a method of cultivating motherwort by symbiotically cultivating plants and rhizosphere growth-promoting bacteria can be tried to solve the problems of low seedling growth rate and poor growth in current motherwort cultivation. However, the selection of rhizosphere growth-promoting bacteria is key. Summary of the Invention

[0006] The technical problem to be solved by the present invention is to provide a bacterium P6-4 and a motherwort growth-promoting agent, as well as their preparation method and application. The bacterium P6-4 is screened and obtained from the soil in the Nvshan volcanic area of ​​Anhui Province. It is used to prepare a growth-promoting agent and used for the growth of motherwort seedlings, which can promote the adaptation of motherwort seedlings to the environment, promote their absorption of soil nutrients and water, accelerate the development and growth of seedlings, and solve the problems of low seedling growth rate and poor growth of seedlings in the current motherwort planting.

[0007] The present invention adopts the following technical solutions to solve the above technical problems:

[0008] A phage strain P6-4, which was taxonomically identified as Variovorax sp., was deposited in the China Center for Type Culture Collection in Wuhan, China on May 6, 2025 and appeared to be alive, with the deposit number CCTCCNO: M2025958.

[0009] As one of the preferred embodiments of the present invention, the 16S rRNA gene sequence of the strain P6-4 is shown as SEQ ID NO.1.

[0010] A motherwort growth-promoting bacterial agent comprises Variovorax and LB liquid culture medium; the Variovorax is the above-mentioned Variovorax P6-4.

[0011] As one of the preferred embodiments of the present invention, the composition of the LB liquid culture medium ( / L) is as follows: 5 g YEASTEXTRACT, 10 g NaCl, and 10 g peptone.

[0012] As one of the preferred embodiments of the present invention, in the Leonurus japonicus growth-promoting agent, the OD of the phagocytic bacteria is 600 The value is 0.95 to 1.05, and more preferably 1.00.

[0013] A preparation method of the above-mentioned motherwort growth-promoting agent comprises inoculating target bacteria into the culture medium at a volume ratio of phagocyte P6-4 to LB liquid culture medium of 1:(100-200), and finally culturing in the dark at 25-28°C.

[0014] As one of the preferred embodiments of the present invention, the volume ratio of the phage P6-4 to the LB liquid culture medium is specifically 1:200.

[0015] An application of the above-mentioned motherwort growth-promoting bacterial agent in promoting the growth of motherwort seedlings.

[0016] A method for promoting the growth of motherwort seedlings comprises applying the motherwort growth-promoting agent to the motherwort seedlings.

[0017] As one of the preferred embodiments of the present invention, the present invention comprises the following specific steps:

[0018] (1) Prepare a planting pit using black soil from Zingiber officinale;

[0019] (2) Sow motherwort seeds in the planting pit, cover with soil, and water;

[0020] (3) After the motherwort seeds germinate and the motherwort seedlings grow, pour the motherwort growth-promoting agent into the plants.

[0021] As one of the preferred embodiments of the present invention, in step (1), the length × width × depth of the planting pit is (1.5-2.5 cm) × (1.5-2.5 cm) × (1-3 cm), more preferably 2 cm × 2 cm × 1.5 cm; the pit distance is 8-10 cm, more preferably 8 cm.

[0022] As one of the preferred embodiments of the present invention, in step (2), the thickness of the covering soil is 1 to 3 cm, more preferably 1.5 cm.

[0023] As one of the preferred embodiments of the present invention, in step (2), watering is to pour clean water after covering the soil to allow water to penetrate, and cover the ground with a film to prevent the surface soil from drying out. Watering is done once a day, and each time watering is done until the soil surface is moist.

[0024] As one of the preferred embodiments of the present invention, in step (3), the amount of the motherwort growth-promoting agent poured in is 8 to 12 mL / time, more preferably 10 mL / time.

[0025] The advantages of the present invention over the prior art are:

[0026] (1) The present invention screened and obtained a Variovorax sp. P6-4 from the soil of the Nvshan volcanic area in Anhui Province. By applying a motherwort growth-promoting agent based on this strain to the growth of motherwort seedlings, it was found that it can promote the establishment of a symbiotic relationship between motherwort and plant rhizosphere growth-promoting bacteria, promote the seedlings' absorption of soil nutrients and water, accelerate the development and growth of seedlings, and solve the problems of low seedling growth rate and poor seedling growth in the current motherwort planting. This is of great significance to the planting of motherwort and the benefits of using motherwort as a raw material for pharmaceutical production.

[0027] (2) The method of the motherwort growth-promoting agent based on the bacterium P6-4 used in the present invention is easy to operate and has a simple process, which is more convenient for agricultural and forestry workers to implement;

[0028] (3) The present invention uses green and pollution-free products that do not pollute the environment and produce beneficial plant-plant rhizosphere growth-promoting bacterial symbionts, which play an important role in maintaining ecological balance. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 is a morphological diagram of the strain P6-4 in Experimental Example 2;

[0030] Figure 2 This is a comparison of the growth of Leonurus japonicus in the blank control group (CK), experimental group (P) and commercially available Bacillus subtilis control group (K) in Experimental Example 3;

[0031] Figure 3This is a comparison chart of the total fresh weight results of Leonurus japonicus in the blank control group (CK), experimental group (P) and commercially available Bacillus subtilis control group (K) in Experimental Example 3;

[0032] Figure 4 This is a comparison chart of the fresh weight of the aboveground part of Leonurus japonicus in the blank control group (CK), experimental group (P) and commercially available Bacillus subtilis control group (K) in Experimental Example 3;

[0033] Figure 5 This is a comparison chart of the total dry weight results of Leonurus japonicus in the blank control group (CK), experimental group (P) and commercially available Bacillus subtilis control group (K) in Experimental Example 3;

[0034] Figure 6 This is a comparison chart of the dry weight results of the aboveground part of Leonurus japonicus in the blank control group (CK), experimental group (P) and commercially available Bacillus subtilis control group (K) in Experimental Example 3;

[0035] Figure 7 This is a comparison chart of the total length results of Leonurus japonicus in the blank control group (CK), experimental group (P) and commercially available Bacillus subtilis control group (K) in Experimental Example 3;

[0036] Figure 8 This is a comparison chart of the plant height results of Leonurus japonicus in the blank control group (CK), experimental group (P) and commercially available Bacillus subtilis control group (K) in Experimental Example 3;

[0037] Figure 9 This is a comparison chart of the root length results of Leonurus japonicus in the blank control group (CK), experimental group (P) and commercially available Bacillus subtilis control group (K) in Experimental Example 3;

[0038] Figure 10 This is a comparison chart of the leaf area results of Leonurus japonicus in the blank control group (CK), experimental group (P) and commercially available Bacillus subtilis control group (K) in Experimental Example 3. DETAILED DESCRIPTION

[0039] The following embodiments of the present invention are described in detail. These embodiments are implemented based on the technical solutions of the present invention, and detailed implementation methods and specific operating procedures are given. However, the scope of protection of the present invention is not limited to the following embodiments. At the same time, in the following embodiments and experimental examples, the reagents and materials used are conventional reagents and materials in the field unless otherwise specified; the experimental conditions and experimental methods used are conventional conditions and methods in the field unless otherwise specified, and will not be repeated here.

[0040] The culture medium formulas involved in the following examples and experimental examples are as follows:

[0041] LB liquid medium: 5 g YEAST EXTRACT, 10 g NaCl, 10 g peptone, dilute to 1 L with distilled water, adjust the pH to about 7.0, and sterilize at 121°C for 21 min.

[0042] Solid culture medium for phosphate-solubilizing bacteria: 1000ml of pure water, 10g of glucose, 0.3g of NaCl, 0.3g of KCl, 10g of Ca3(PO4)2, 0.5g of (NH4)2SO4, 2g of K2HPO4, 0.3g of MgSO4·7H2O, 0.03g of FeSO4﹒7H2O, 0.03g of MnSO4·4H2O, 18-20g of agar, adjust the pH to 7.2, sterilize at 121℃ for 20min.

[0043] Example 1

[0044] The phagophora P6-4 of this example was screened and obtained from the soil in the Nvshan volcanic area of ​​Anhui Province. Its 16S rRNA gene sequence is shown in SEQ ID NO.1, and it was classified and identified as Variovorax sp. It was deposited in the China Center for Type Culture Collection in Wuhan, China on May 6, 2025 and showed survival, with the deposit number CCTCC NO: M2025958.

[0045] Example 2

[0046] The motherwort growth-promoting bacterial agent of this embodiment includes Variovorax and LB liquid culture medium; wherein, Variovorax is Variovorax P6-4 of Example 1.

[0047] Preparation method:

[0048] (1) Take out the tube containing the test strain, Phage P6-4, from the ultra-low temperature freezer and dissolve it at room temperature for 5 minutes.

[0049] (2) In a clean bench, inoculate the culture medium with the phage P6-4 solution at a volume ratio of 1:200.

[0050] (3) The culture medium inoculated with phage P6-4 was placed in a dark incubation at 25°C for 2 days, and the OD of the bacterial solution was measured. 600 value;

[0051] (4) Dilute the bacterial solution with distilled water to the OD value of the phage P6-4. 600 A value of 1.0 indicates that the target desired motherwort growth-promoting agent is obtained.

[0052] Example 3

[0053] The motherwort growth-promoting agent of this embodiment is basically the same as that of Example 2, with the main difference being that in the preparation method:

[0054] (1) Take out the tube containing the test strain, Phage P6-4, from the ultra-low temperature freezer and dissolve it at room temperature for 5 minutes.

[0055] (2) In a clean bench, inoculate the culture medium with the phage P6-4 bacterial solution and LB liquid culture medium at a volume ratio of "1:150".

[0056] (3) The culture medium inoculated with phage P6-4 was placed in a dark incubation at 27°C for 2 days, and the OD of the bacterial solution was measured. 600 value;

[0057] (4) Dilute the bacterial solution with distilled water to the OD value of the phage P6-4. 600 A value of 1.0 indicates that the target desired motherwort growth-promoting agent is obtained.

[0058] Example 4

[0059] The motherwort growth-promoting agent of this embodiment is basically the same as that of Example 2, with the main difference being that in the preparation method:

[0060] (1) Take out the tube containing the test strain, Phage P6-4, from the ultra-low temperature freezer and dissolve it at room temperature for 5 minutes.

[0061] (2) In a clean bench, inoculate the culture medium with the phage P6-4 solution at a volume ratio of 1:100.

[0062] (3) The culture medium inoculated with phage P6-4 was placed in a dark incubation at 25°C for 2 days, and the OD of the bacterial solution was measured. 600 value.

[0063] (4) Dilute the bacterial solution with distilled water to the OD value of the phage P6-4. 600 The value is 0.95, which means the motherwort growth-promoting agent required for the target is obtained.

[0064] Example 5

[0065] The motherwort growth-promoting agent of this embodiment is basically the same as that of Example 2, with the main difference being that in the preparation method:

[0066] (1) Take out the tube containing the test strain, Phage P6-4, from the ultra-low temperature freezer and dissolve it at room temperature for 5 minutes.

[0067] (2) In a clean bench, inoculate the culture medium with the phage P6-4 solution at a volume ratio of 1:200.

[0068] (3) The culture medium inoculated with phage P6-4 was placed in a dark incubation at 28°C for 2 days, and the OD of the bacterial solution was measured. 600 value.

[0069] (4) Dilute the bacterial solution with distilled water to the OD value of the phage P6-4. 600 The value is 1.05, which means the motherwort growth-promoting agent required for the target is obtained.

[0070] Example 6

[0071] A method for growing motherwort in this embodiment:

[0072] (1) Prepare planting pits using black soil of sand ginger. The length × width × depth of the planting pits are 2 cm × 2 cm × 1.5 cm; the pit spacing is 8 cm.

[0073] (2) Sow motherwort seeds in the planting pit.

[0074] (3) After planting the motherwort seeds, cover with 1.5 cm of soil and water them; pour 200 mL of clean water per plant to allow water to penetrate. Cover with mulch to prevent the topsoil from drying out. Remove the mulch after germination. Thereafter, water once a day, 50 mL each time, to keep the soil moist. During this period, the temperature and light intensity should be based on the natural conditions in the greenhouse (the greenhouse temperature is maintained at 26°C and the average daily light intensity is 11.5 hours).

[0075] (4) After 7 days of germination, the motherwort growth-promoting agent of the above embodiment was poured into the motherwort seedlings at a rate of 10 mL / time, and the amount of watering was once every 3 days.

[0076] Example 7

[0077] A method for growing motherwort in this embodiment:

[0078] (1) Planting pits were prepared using black soil from sand ginger. The length × width × depth of the planting pits were 1.5 cm × 1.5 cm × 1 m, more preferably 2 cm × 2 cm × 1.5 cm. The pit spacing was 9 cm.

[0079] (2) Sow motherwort seeds in the planting pit.

[0080] (3) After planting the motherwort seeds, cover with 1 cm of soil and water them; pour 200 mL of clean water per plant to allow water to penetrate. Cover with mulch to prevent the topsoil from drying out. Remove the mulch after germination. Thereafter, water once a day, 50 mL each time, to keep the soil moist. During this period, the temperature and light intensity should be based on the natural conditions in the greenhouse (the greenhouse temperature should be maintained at 26°C and the average daily light intensity should be 11.5 hours).

[0081] (4) After 7 days of germination, the motherwort growth-promoting agent of the above embodiment was poured into the motherwort seedlings at a rate of 8 mL per time, and the amount of watering was once every 3 days.

[0082] Example 8

[0083] A method for growing motherwort in this embodiment:

[0084] (1) Prepare planting pits using black soil of sand ginger; the length × width × depth of the planting pits are 2.5 cm × 2.5 cm × 3 cm, and the pit distance is 10 cm.

[0085] (2) Sow motherwort seeds in the planting pit.

[0086] (3) After planting the motherwort seeds, cover with 3 cm of soil and water them; pour 200 mL of clean water per plant to allow water to penetrate. Cover with mulch to prevent the topsoil from drying out. Remove the mulch after germination. Thereafter, water once a day, 50 mL each time, to keep the soil moist. During this period, the temperature and light intensity should be based on the natural conditions in the greenhouse (the greenhouse temperature is maintained at 26°C and the average daily light intensity is 11.5 hours).

[0087] (4) After 7 days of germination, the motherwort growth-promoting agent of the above embodiment was poured into the motherwort seedlings at a rate of 12 mL / time, and the amount of watering was once every 3 days.

[0088] Experimental Example 1: Screening and Isolation of Strain P6-4:

[0089] The laboratory previously screened a strain with phosphate-solubilizing ability from the soil of the Nvshan volcanic area in Anhui Province. The screening process was as follows:

[0090] (1) Preparation of soil suspension

[0091] Weigh 10g of soil from the Nvshan volcanic area of ​​Anhui Province and name it LD20. Place the LD20 sample in a 250ml Erlenmeyer flask, add 90ml of sterile water, and shake in a constant temperature shaker at 28°C and 180 rpm for 1 hour. Perform a gradient dilution of the soil suspension by pipetting 0.9ml of the soil suspension into a centrifuge tube, adding 8.1ml of sterilized pure water, and mixing thoroughly to obtain a concentration of 10. -1 By repeating this step, a soil suspension with a concentration of 10 -4, 10 -5 , 10 -6 of soil suspension.

[0092] (2) Preliminary screening

[0093] Initial screening of strains with phosphate solubilization ability was performed using solid phosphate solubilization medium. -4 , 10 -5 , 10 -6 Apply 150 μl of each LD20 soil suspension sample to the culture medium using a spreading rod. To ensure accurate results, perform three replicates for each concentration gradient. Place the coated culture medium in a constant temperature incubator at 29°C for 3–4 days.

[0094] (3) Rescreening:

[0095] Colonies that grew well and exhibited significant morphological differences were purified using the three-zone streak and punch methods and cultured for 3 days. This purification process was repeated 2–3 times until a single colony was identified. The purified strains were stored at -80°C using glycerol.

[0096] Experimental Example 2, Identification of strain P6-4:

[0097] Based on Experimental Example 1, a new strain with phosphate solubilization ability was finally screened and named strain P6-4.

[0098] 1. Strain morphology

[0099] Figure 1 shown.

[0100] 2. 16S rRNA gene sequence

[0101] The 16S rRNA gene sequence of strain P6-4 is shown in SEQ ID NO.1.

[0102] 3. Preservation and further identification

[0103] Combined with morphological and physiological and biochemical identification, strain P6-4 was identified as a new species of Variovorax sp., officially named Variovorax sp. P6-4, and deposited in the China Center for Type Culture Collection in Wuhan, China on May 6, 2025, and showed survival, with the deposit number CCTCC NO: M2025958.

[0104] Since the phosphorus-solubilizing ability of bacteria is one of the key points in the symbiotic relationship between plants and plant rhizosphere growth-promoting bacteria, the present invention uses the phage P6-4 for the preparation of motherwort growth-promoting bacteria agent and motherwort planting experiment.

[0105] Experimental Example 3: Verification experiment on the growth-promoting effect of the growth-promoting bacteria agent of the present invention on the growth of motherwort seedlings:

[0106] 1. Experimental methods:

[0107] (1) Prepare nine pots of black soil for sand ginger, set up three planting holes in each pot, the length × width × depth of the planting hole is 2cm × 2cm × 1.5cm, and the pit distance is 8cm.

[0108] (2) Plant one motherwort seed in each planting hole.

[0109] (3) After planting the motherwort seeds, cover with 1.5 cm of soil and water them; pour 200 mL of clean water per plant to allow water to penetrate. Cover with mulch to prevent the topsoil from drying out. Remove the mulch after germination. Thereafter, water once a day, 50 mL each time, to keep the soil moist. During this period, the temperature and light intensity should be based on the natural conditions in the greenhouse (the greenhouse temperature is maintained at 26°C and the average daily light intensity is 11.5 hours).

[0110] (4) After 7 days of germination, the motherwort plants were divided into a blank control group (CK), an experimental group (P), and a commercially available Bacillus subtilis control group (K) for separate treatments.

[0111] Experimental group (3 pots): The Leonurus japonicus seedlings of the corresponding group were irrigated with the Leonurus japonicus growth-promoting bacterial agent of the present invention (taking the bacterial agent of Example 2 as an example) at an injection volume of 10 mL / time, once every 3 days.

[0112] Commercially available Bacillus subtilis control group (3 pots): Commercially available Bacillus subtilis was poured into the Leonurus japonicus seedlings of the corresponding group at a rate of 10 mL / time, once every 3 days.

[0113] Blank control group (3 pots): no growth-promoting agents were applied.

[0114] (5) One month later, the fresh weight (including total fresh weight and fresh weight of aboveground parts), dry weight (including total dry weight and dry weight of aboveground parts), total length, plant height (aboveground parts), root length, and leaf area of ​​Leonurus japonicus in the blank control group (CK), experimental group (P), and commercially available Bacillus subtilis control group (K) were statistically analyzed.

[0115] 2. Experimental results:

[0116] The growth of motherwort in the blank control group (CK), experimental group (P) and commercial Bacillus subtilis control group (K) is shown in Figure 2. Figure 2 The results of total fresh weight and aboveground fresh weight are shown in Figure 3 、 4 The total dry weight and aboveground dry weight results are shown in Figure 5 、 6The results of total length, plant height and root length are shown in Figure 7 、 8 , 9, the leaf area results are as follows Figure 10 shown.

[0117] The above results showed that: (1) compared with the blank control group (CK), the total fresh weight and aboveground fresh weight of the experimental group (P) increased by an average of 29.01% and 35.88%, respectively; (2) compared with the blank control group (CK), the total dry weight and aboveground dry weight of the experimental group (P) increased by an average of 47.82% and 55.30%, respectively; (3) compared with the blank control group (CK), the total length, plant height and root length of the experimental group (P) increased by an average of 28.60%, 46.31% and 33.86%, respectively; (4) compared with the blank control group (CK), the leaf area of ​​the experimental group (P) increased by an average of 31.22%; (5) compared with the commercially available Bacillus subtilis control group (K), the experimental group (P) had a better overall effect.

[0118] The above results show that inoculation of the motherwort growth-promoting agent based on the bacterium P6-4 of the present invention can significantly promote the growth of motherwort seedlings, increase the fresh weight, dry weight, length and leaf area of ​​motherwort, and the effect is better than that of commercially available Bacillus subtilis.

[0119] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A phage P6-4, characterized in that: It was classified and identified as Variovorax sp. and deposited in China Center for Type Culture Collection with the deposit number CCTCC NO: M2025958.

2. The phage P6-4 according to claim 1, characterized in that The 16S rRNA gene sequence of the strain P6-4 is shown in SEQ ID NO.

1.

3. A motherwort growth-promoting agent, characterized in that It comprises phage and LB liquid culture medium; the phage is the phage P6-4 described in claim 1.

4. The motherwort growth-promoting agent according to claim 3, characterized in that In the motherwort growth-promoting agent, the OD of the phagocytic bacteria 600 The value is 0.95~1.

05.

5. A method for preparing the motherwort growth-promoting agent according to claim 3 or 4, characterized in that: The target bacteria were inoculated into the culture medium at a volume ratio of 1:(100-200) of the phage P6-4 to the LB liquid culture medium, and the culture medium was finally placed in the dark at 25°C-28°C.

6. Use of the motherwort growth-promoting agent according to claim 3 or 4 in promoting the growth of motherwort seedlings.

7. A method for promoting the growth of motherwort seedlings, characterized in that: Apply the motherwort growth-promoting fungus agent according to claim 3 or 4 to the motherwort seedlings.

8. The method for promoting the growth of motherwort seedlings according to claim 7, characterized in that: The specific steps include: (1) Prepare a planting pit using black soil from Zingiber officinale; (2) Sow motherwort seeds in the planting pit, cover with soil, and water; (3) After the motherwort seeds germinate and the motherwort seedlings grow, pour the motherwort growth-promoting agent into the plants.

9. The method for promoting the growth of motherwort seedlings according to claim 7, characterized in that: In the step (2), the thickness of the covering soil is 1 to 3 cm.

10. The method for promoting the growth of Leonurus japonicus seedlings according to claim 7, characterized in that: In the step (3), the amount of the motherwort growth-promoting agent poured in is 8 to 12 mL per time.

Images