Ganoderma lucidum spore oil detection and identification method based on coenzyme Q9 and tetracosahexaenoic acid

CN120652009APending Publication Date: 2025-09-16JIANGSU BEEVIP BIOTECHNOLOGY CO LTD JIANGSU
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Patent Information

Application Number
CN202511015466.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-07-23
Publication Date
2025-09-16

AI Technical Summary

Technical Problem

Existing Ganoderma lucidum spore oil detection methods cannot effectively distinguish authenticity, traditional physical and chemical index detection cannot specifically identify adulteration, fingerprint atlas method has poor repeatability and is difficult to quantify, and single marker detection is easily forged.

Method used

Coenzyme Q9 and tetracosahexanoic acid were used as dual markers, combined with high performance liquid chromatography-mass spectrometry technology to establish a standard range of dual marker content. Through sample pretreatment, solid phase extraction cleanup and isotope internal standard addition, the authenticity identification and quality control of Ganoderma lucidum spore oil were achieved.

Benefits of technology

The specificity and accuracy of Ganoderma lucidum spore oil detection have been improved, with the detection limit as low as 0.98 mg/kg and a wide linear range, which meets the needs of industrial quality control and reduces the false positive rate. It is suitable for the quality identification of Ganoderma lucidum spore oil from different production areas.

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Abstract

The invention relates to a high performance liquid chromatography-mass spectrometry technology, in particular to a ganoderma lucidum spore oil detection and identification method, which comprises the following steps: mixing a sample with ethanol, adding vitamin E and EDTA (Ethylene Diamine Tetraacetic Acid), performing ultrasonic extraction, performing refrigerated centrifugation, purifying through a silica column, and performing rotary evaporation redissolution; after an isotope internal standard is added, detecting the content of coenzyme Q9 and tetracosahexaenoic acid by HPLC-MS (High Performance Liquid Chromatography-Mass Spectrometer); and comparing with a standard range, if the double indexes are qualified and the ratio reaches the standard, determining that the product is true. Double markers are combined with mass spectrum detection, the problem that adulteration is difficult to distinguish is solved, the detection limit is low, the precision is high, oxidation is prevented through pretreatment, production areas can be distinguished, and a reliable means is provided for supervision and quality control.
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