Centrifugal column chromatography extraction device and method

Abstract

The invention belongs to the technical field of chromatographic separation and preparation, and particularly relates to a centrifugal column chromatographic extraction device and method. The device comprises a chromatographic column, an extraction bin, a top cover box, a tubular column and an external connecting assembly, the top cover box and the external connecting assembly are arranged at the top and the bottom of the extraction bin, the tubular column penetrates through the top cover box and is rotationally connected with the top cover box through a bearing assembly, the chromatographic column is contained in the extraction bin and connected with the tubular column, and a sample solution is injected into the chromatographic column through the tubular column. Then pressurizing and injecting a mobile phase, driving the chromatographic column to rotate by the tubular column for chromatographic separation, pressurizing and injecting an extracting solution into the extraction bin, and discharging the separated mobile phase by the extracting solution through an external connecting assembly. While pressurized separation is carried out, high-speed rotating centrifugation of the chromatographic column is utilized, centrifugal force is used as auxiliary drive, mass transfer resistance is reduced, separation efficiency is improved, and a high-efficiency mode and way can be provided for rapid separation and extraction of samples in life science, biological detection and food and drug detection.

Description

Technical Field

[0001] The invention belongs to the technical field of chromatographic separation and preparation, and particularly relates to a centrifugal column chromatographic extraction device and method. Background Art

[0002] The material research of samples depends on the analysis and distribution research of the material components in the sample system. The extraction, purification and enrichment of the materials therein are the key to determining the occurrence form and quantification of the materials in the sample. Traditional chromatography has long been used under pressure conditions, through the flow of reagents, at a certain linear velocity, to carry out interphase adsorption or distribution of samples, so that related substances can be transformed and extracted from other coexisting material systems into the corresponding simple reagent system. However, the separation efficiency of chromatography is subject to the effect of mass transfer and distribution. For substances in complex material systems, many have very similar chromatographic separation behaviors. Affected by the above factors, more separation and matrix conversion processes will have bottlenecks. Similar environments or similar substances are difficult to be completely separated and extracted, affecting subsequent inspection, identification and distribution analysis. In addition, the traditional chromatographic process has a relatively fixed processing time. For the detailed analysis of complex material systems, it may take longer to extract and separate. These have affected the corresponding inspection and detection efficiency, limited the refined application of chromatography, and formed a bottleneck in inspection. Summary of the Invention

[0003] In response to the above problems, the purpose of the present invention is to provide a centrifugal column chromatography extraction device and method to solve the problems of low separation efficiency in traditional chromatographic analysis processes, difficulty in separating complex material systems and long processing time, and difficulty in completely separating similar environments or similar substances.

[0004] In order to achieve the above object, the present invention adopts the following technical solutions:

[0005] On the one hand, the present invention provides a centrifugal column chromatography extraction device, comprising a chromatography column, an extraction chamber, a top cover box, a tubular column and an external connection component, wherein the top cover box and the external connection component are respectively arranged at the top and bottom of the extraction chamber, the tubular column passes through the top cover box and is rotatably connected to the top cover box through a bearing assembly, the chromatography column is accommodated in the extraction chamber and connected to the tubular column, the sample solution is injected into the chromatography column by the tubular column, and then the mobile phase is pressurized and injected, and the chromatography column is driven by the tubular column to rotate for chromatographic separation, and at the same time, the extraction liquid is pressurized and injected into the extraction chamber, and the extraction liquid carries the separated mobile phase and is discharged through the external connection component.

[0006] The chromatographic column includes a cylindrical inner net and a cylindrical outer net, the upper end of the cylindrical inner net is connected to the tube column; the cylindrical outer net is enclosed on the outside of the cylindrical inner net, and an annular cavity for filling chromatographic filler is left between the cylindrical outer net and the cylindrical inner net, and the cylindrical inner net and the cylindrical outer net are used for gradient filtration of the mobile phase.

[0007] The cylindrical inner mesh has uniformly arranged 0.22μ mesh holes; the cylindrical outer mesh has uniformly arranged 0.45μ mesh holes.

[0008] The extraction bin is a cylindrical container, an upper sieve plate is provided between the top of the extraction bin and the top cover box, and a lower sieve plate is provided between the bottom of the extraction bin and the external connection assembly.

[0009] The pipe string is rotatably connected to an external pipe through a connecting module. A backup pressure column made of porous material is provided in the external pipe. The backup pressure column is used to increase the backup pressure in the pipe string.

[0010] The top cover box is provided with an injection tube, the upper end of which is connected to the back pressure column and the connecting tube in sequence. The back pressure column is a filtering column formed of a porous material, and the back pressure column is used to increase the back pressure in the extraction chamber;

[0011] The back pressure in the extraction chamber is less than the backup pressure value in the column to ensure that the flow direction of the chromatographic reagent is unidirectional.

[0012] The bearing assembly includes an upper bearing and a lower bearing stacked up and down, and a sealing ring disposed between the inner rings of the upper bearing and the lower bearing. The sealing ring is in close contact with the pipe string through an elastic spring structure contained therein;

[0013] The upper bearing and the lower bearing are respectively embedded in the upper bearing buckle box and the lower bearing buckle box, and the upper bearing buckle box and the lower bearing buckle box are connected by a buckle.

[0014] The external connection component includes a connector, an adapter and an external interface connected in sequence, wherein the connector is a hollow structure, the upper end of the connector is sealed with the bottom of the extraction chamber, the lower end of the connector is connected to the external interface through the adapter, and the external interface is connected to the collector or detection device.

[0015] The centrifugal column chromatography extraction device also includes a rotary drive mechanism; the rotary drive mechanism includes a motor, a belt and two pulleys, wherein the motor is arranged on the top cover box, and the two pulleys are respectively arranged at the output end of the motor and on the column, and the two pulleys are connected by belt transmission.

[0016] Another aspect of the present invention provides a centrifugal column chromatography extraction method using the above device, comprising the following steps:

[0017] Inject the sample solution into the chromatographic column through the tubing;

[0018] The tubing drives the chromatographic column to rotate;

[0019] Inject the extraction liquid into the extraction chamber to keep the back pressure value in the extraction chamber lower than the back pressure value in the column;

[0020] The separated fractions are collected at the bottom of the extraction chamber.

[0021] The advantages and beneficial effects of the present invention are as follows: the present invention provides a centrifugal column chromatography extraction device, which separates, purifies, and extracts substances through high-speed centrifugal mass transfer in the chromatographic separation part under the traditional mass transfer process, combined with distribution and adsorption. The high-speed rotation centrifugation of the chromatographic column introduces centrifugal force as an auxiliary drive, reduces mass transfer resistance, and improves separation efficiency, which can provide a high-efficiency method and approach for the rapid separation and extraction of samples in life sciences, biological testing, and food and drug testing. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a schematic structural diagram of a centrifugal column chromatography extraction device of the present invention;

[0023] Figure 2 A top view of the top cover box of the present invention;

[0024] Figure 3 It is a side view of the top cover box of the present invention;

[0025] Figure 4 Schematic diagram of the structure of the chromatographic column in the present invention;

[0026] Figure 5 Schematic diagram of the structure of the bearing assembly of the present invention;

[0027] Figure 6 It is a structural schematic diagram of the pipeline connection module in the present invention.

[0028] In the figure: 1. Chromatographic column; 2. Connector; 3. Adapter; 4. External interface; 5. Lower sieve plate; 6. Extraction chamber; 7. Cylindrical outer mesh; 8. Upper sieve plate; 9. Top cover box; 10. Injection tube; 11. Back pressure column; 12. Connecting tube; 13. Infusion external interface; 14. External pipeline; 15. External pipeline inlet; 16. Connecting module; 17. Pipe column; 18. Bearing assembly; 19. Sealing ring; 20. Cylindrical inner mesh; 21. Backup pressure column; 22. Bearing housing; 23. Ball bearing; 24. Upper bearing; 25. Lower bearing; 27. Motor; 28. Belt. DETAILED DESCRIPTION

[0029] In order to make the objectives, technical solutions and advantages of the present invention more clear, the present invention is described in detail below with reference to the accompanying drawings and specific embodiments.

[0030] See also Figures 1 to 6As shown, an embodiment of the present invention provides a centrifugal column chromatography extraction device, comprising a chromatography column 1, an extraction chamber 6, a top cover box 9, a tubular column 17 and an external connection assembly, wherein the top cover box 9 and the external connection assembly are respectively arranged at the top and bottom of the extraction chamber 6, the tubular column 17 passes through the top cover box 9 and is rotatably connected to the top cover box 9 through a bearing assembly 18, the chromatography column 1 is accommodated in the extraction chamber 6 and connected to the tubular column 17, and the chromatography column 1 is filled with corresponding chromatography fillers for separation; the sample solution is injected into the chromatography column 1 by the tubular column 17, and then the mobile phase is pressurized and injected, and the chromatography column 1 is driven by the tubular column 17 to rotate for chromatographic separation, and at the same time, the extraction chamber 6 is pressurized and injected with an additional extract for collection and use, and the extract carries the separated mobile phase and is discharged by the external connection assembly.

[0031] See also Figure 1 As shown, in the embodiment of the present invention, the extraction chamber 6 is a cylindrical container, with an upper sieve plate 8 disposed between the top of the extraction chamber 6 and the top cover box 9, and a lower sieve plate 5 disposed between the bottom of the extraction chamber 6 and the external connection assembly. The external connection assembly includes a connector 2, an adapter 3, and an external interface 4, which are connected in sequence. The connector 2 is a hollow structure, with the upper end of the connector 2 sealed to the bottom of the extraction chamber 6, and the lower end of the connector 2 connected to the external interface 4 via the adapter 3. The external interface 4 is connected to a collector or detection device.

[0032] See also Figure 2 As shown, based on the above embodiment, a centrifugal column chromatography extraction device provided by the present invention also includes a rotation drive mechanism for driving the column 17 and the chromatography column 1 to rotate; the rotation drive mechanism includes a motor 27, a belt 28 and two pulleys, wherein the motor 27 is arranged on the top cover box 9, and the two pulleys are respectively arranged on the output end of the motor 27 and the column 17, and the two pulleys are connected by the belt 28. The motor 27 drives the column 17 and the chromatography column 1 to rotate at high speed through the belt 28.

[0033] See also Figure 1 and Figure 4 As shown, in an embodiment of the present invention, the chromatographic column 1 includes a cylindrical inner mesh 20 and a cylindrical outer mesh 7. The upper end of the cylindrical inner mesh 20 is connected to the column 17, and the cylindrical outer mesh 7 is enclosed on the outside of the cylindrical inner mesh 20. An annular cavity for filling chromatographic filler is left between the cylindrical outer mesh 7 and the cylindrical inner mesh 20. The cylindrical inner mesh 20 and the cylindrical outer mesh 7 are used for gradient filtration of the mobile phase.

[0034] Specifically, the top and bottom of the cylindrical outer mesh 7 are arc-shaped caps without holes, making the chromatographic column 1 have a capsule-shaped structure. The cylindrical inner mesh 20 has uniformly arranged 0.22μ mesh holes; the cylindrical outer mesh 7 has uniformly arranged 0.45μ mesh holes.

[0035] See also Figure 1 and Figure 4As shown, in an embodiment of the present invention, the pipe string 17 is rotatably connected to the external pipe 14 through a connecting module 16 , and a backup pressure column 21 made of porous material is provided in the external pipe 14 , and the backup pressure column 21 is used to increase the backup pressure in the pipe string 17 .

[0036] See also Figure 6 As shown, in an embodiment of the present invention, the connection module 16 includes a bearing housing 22 and a ball bearing 23 embedded in the bearing housing 22. The bearing housing 22 is connected to the external pipe 14. The ball bearing 23 is mounted on the pipe column 17. The pipe column 17 can rotate relative to the external pipe 14, and there is a small gap between the rotating and stationary pipe heads.

[0037] See also Figures 1 to 3 As shown, in an embodiment of the present invention, two injection tubes 10 are symmetrically provided on the top cover box 9. The injection tubes 10 are channels for injecting the extract into the extraction chamber 6. The upper ends of the injection tubes 10 are connected to the back pressure column 11 and the connecting tube 12 in sequence. The end of the connecting tube 12 is the external infusion interface 13; the back pressure column 11 is a filtering column formed of a porous material, and the back pressure column 11 is used to increase the back pressure in the extraction chamber 6; the back pressure in the extraction chamber 6 is less than the standby pressure value in the column 17 to ensure that the flow direction of the mobile phase is unidirectional.

[0038] Specifically, the top cover box 9 has a through hole in the middle through which the tube column 17 can pass. The cylindrical inner net 20 is bounded by the lower edge of the top cover box 9. The lower edge of the top cover box 9 is arc-shaped and is sealed with the extraction chamber 6 through the upper sieve plate 8. The upper sieve plate 8 has a central through hole through which the tube column 17 can pass. The injection tube 10 is tightly connected to the upper sieve plate 8 so that the injected solution can be evenly dispersed into the extraction chamber 6.

[0039] See also Figure 1 and Figure 5 As shown, in an embodiment of the present invention, the bearing assembly 18 includes an upper bearing 24 and a lower bearing 25 stacked up and down, and a sealing ring 9 arranged between the inner rings of the upper bearing 24 and the lower bearing 25. The sealing ring 9 is in close contact with the pipe column 17 through the elastic spring structure contained therein.

[0040] Furthermore, the upper bearing 24 and the lower bearing 25 are respectively embedded in the upper bearing buckle box and the lower bearing buckle box, and the upper bearing buckle box and the lower bearing buckle box are connected by a snap buckle.

[0041] An embodiment of the present invention provides a centrifugal column chromatography extraction device, the working principle of which is:

[0042] In actual operation, the external device can inject the sample solution and mobile phase through the column 17. At the same time, due to the presence of a certain standby pressure in the column 17, the sample has a fixed flow direction. The sample is separated by the chromatographic column 1 and injected into the extraction chamber 6. The extraction chamber 6 can be injected with an extracting liquid with a certain back pressure by the injection tube 10, and the extracting liquid drives the separated mobile phase to flow out from the external interface 4 at the bottom of the extraction chamber 6. The back pressure value in the extraction chamber 6 is less than the standby pressure value in the column 17 to ensure that the flow direction of the mobile phase is unidirectional. In addition, during the injection separation and extraction process, the transmission device is driven by a rotating motor to rotate the column 17 and the chromatographic column 1 at high speed, thereby enhancing the efficiency of separation and extraction. The device of the present invention can provide a high-efficiency method and approach for the rapid separation and extraction of multiple samples in life sciences, biological testing, and food and drug testing.

[0043] Specifically, when filling the chromatographic filler into the annular cavity of the chromatographic column 1, the upper cover of the chromatographic column 1 is temporarily not sealed. After the chromatographic filler slurry is injected and simply pressed up to fill it, the upper cover of the chromatographic column 1 is pressed on, and then the external pipe 14 is connected to the infusion system, the preparation pressure is increased, and the chromatographic column 1 is compacted.

[0044] See also Figures 1 to 6 As shown, based on the above design concept, another embodiment of the present invention provides a centrifugal column chromatography extraction method using the device described in the above embodiment, comprising the following steps:

[0045] The sample solution is injected into the chromatographic column 1 through the external pipe inlet 15 of the column 17 by an external device, and the sample solution flows in a fixed direction with the subsequent mobile phase through the preparation pressure;

[0046] The column 17 drives the chromatographic column 1 to rotate, performs chromatographic separation, and is driven into the extraction chamber 6;

[0047] Injecting an extracting liquid with a certain back pressure into the extraction chamber 6 to keep the back pressure value in the extraction chamber 6 lower than the back pressure value in the column 17;

[0048] The separated components are collected at the bottom of the extraction chamber 6 : the extraction liquid drives the separated mobile phase to flow out from the external connection assembly at the bottom of the extraction chamber 6 .

[0049] The present invention uses centrifugal-assisted driven extraction chromatographic separation, which has better separation efficiency and purification capacity than a general chromatographic column in a static state, and can realize the coupling of rotary concentration and decompression centrifugal concentration for sample preparation and concentration.

[0050] Example 1

[0051] The annular cavity of chromatographic column 1 was filled with C18, 10μ chromatographic packing. Column 1 was 3 cm long, with a 1 cm diameter cylindrical outer mesh 7 and a 0.2 cm diameter cylindrical inner mesh 20. The mobile phase was methanol:water (80:20), and the internal pressure of column 17 was maintained at 110 psi. Extraction chamber 6 had a height of 5 cm and an inner diameter of 2 cm. The upper and lower covers of extraction chamber 6 were designed and adjusted to the specific dimensions. Column 1 was rotated at a speed of 5000-10000 rpm. The extract and mobile phase compositions were consistent, and the back pressure of extraction chamber 6 was controlled at 80 psi. 100 μL of a 0.05% melatonin methanol solution was loaded (the low dead volume design was not considered for outflow). The experimental parameters are as follows:

[0052]

[0053] The above results show that as the rotation speed of the chromatographic column 1 increases, the mass transfer resistance decreases, which makes the mass transfer speed increase significantly.

[0054] Load 100100 μL of 0.05% caffeine and phenol (1:1) methanol solution. The experimental parameters are as follows:

[0055]

[0056] The above results show that for the separation pair, the device can maintain a good separation effect. At the same time, the pressure fluctuates to a certain extent due to the change of mass transfer, but it mainly occurs during the speed change process. After reaching the steady state, the pressure fluctuation is less than 1-2%.

[0057] Example 2

[0058] The annular cavity of the chromatographic column 1 is filled with C18, 10μ chromatographic filler. The length of the chromatographic column 1 is 3cm long. The diameter of the cylindrical outer net 7 of the chromatographic column 1 is 1cm, and the diameter of the cylindrical inner net 20 is 0.2cm. The mobile phase is selected as methanol:water (80:20). The internal pressure of the tubing column 17 is maintained at 110psi. The height of the extraction chamber 6 is 5cm, the internal diameter is 2cm, and the upper and lower covers are designed according to specific size adjustments. The chromatographic column 1 rotates at 5000-10000 rpm, the external injection (extract) is the same as the mobile phase, the back pressure of the extraction chamber 6 is controlled at 80psi, and 100μL of 0.05% gardenia methanol extract is loaded. Because the extraction chamber 6 has a large redundant space outside the chromatographic column 1, it can cause peak broadening. Therefore, a layer of wall-adherent patch was added to the upper part of the outer side of chromatographic column 1, and the dead volume formed was converted into a 75μ inner diameter tube, which is three times the volume of the column of the same length. The experiment was operated at room temperature. The experimental parameters are as follows:

[0059]

[0060] The above data show that the extraction efficiency of complex substances can be greatly improved compared with conventional methods. It can be expected that a greater improvement will be achieved based on higher centrifugation conditions, temperature control and redundant volume adjustment.

[0061] For separation drive and auxiliary accelerated mass transfer, the improvement of mass transfer efficiency is a key aspect in solving difficult separation systems. The related distribution, adsorption, etc. have faster kinetic effects, so they will not be hindered by mass transfer acceleration. On the contrary, when the mass transfer capacity is improved, the kinetic distribution process can be accelerated, the overall number of plates can be increased, a higher separation capacity can be obtained, and a better extraction effect can be obtained. In actual operation, the external equipment can inject the sample solution and mobile phase through the column 17. At the same time, due to the existence of a certain standby pressure, the sample and the mobile phase have a fixed flow direction. They are separated by the chromatographic column 1 and injected into the extraction chamber 6. The extraction chamber 6 can be injected with an extracting liquid with a certain back pressure by the injection tube 10, driving the separated mobile phase to flow out from the lower part of the extraction chamber 6. The back pressure in the extraction chamber 6 is less than the standby pressure value in the column 17 to ensure that the mobile phase flow direction is unidirectional; in addition, during the injection separation and extraction process, the transmission device is driven by the rotating motor to rotate the column 17 and the chromatographic column 1 at high speed, thereby enhancing the efficiency of separation and extraction.

[0062] The present invention provides a centrifugal column chromatography extraction device and method, which separates, purifies, and extracts substances through high-speed centrifugal mass transfer in the chromatographic separation part under the traditional mass transfer process, combined with distribution and adsorption. The high-speed rotation centrifugation of the chromatographic column is driven by the tubing column, and the centrifugal force introduced serves as an auxiliary drive, which reduces the mass transfer resistance and improves the separation efficiency. It can provide a high-efficiency method and approach for the rapid separation and extraction of samples in life sciences, biological testing, and food and drug testing.

[0063] The above description is only an embodiment of the present invention and is not intended to limit the scope of protection of the present invention. Any modification, equivalent replacement, improvement, expansion, etc. made within the spirit and principle of the present invention are included in the scope of protection of the present invention.

Claims

1. A centrifugal column chromatography extraction device, characterized in that, The invention comprises a chromatographic column (1), an extraction chamber (6), a top cover box (9), a tubular column (17) and an external connection component, wherein the top cover box (9) and the external connection component are respectively arranged at the top and bottom of the extraction chamber (6); the tubular column (17) passes through the top cover box (9) and is rotatably connected to the top cover box (9) via a bearing component (18); the chromatographic column (1) is accommodated in the extraction chamber (6) and connected to the tubular column (17); a sample solution is injected into the chromatographic column (1) by the tubular column (17); a mobile phase is then pressurized and injected; the chromatographic column (1) is driven by the tubular column (17) to rotate for chromatographic separation; and at the same time, an extracting liquid is pressurized and injected into the extraction chamber (6); the extracting liquid carries the separated mobile phase and is discharged from the external connection component.

2. The centrifugal column chromatography extraction device according to claim 1, characterized in that The chromatographic column (1) comprises a cylindrical inner net (20) and a cylindrical outer net (7), wherein the upper end of the cylindrical inner net (20) is connected to the tubular column (17); the cylindrical outer net (7) is enclosed on the outside of the cylindrical inner net (20), and an annular cavity for filling chromatographic filler is left between the cylindrical outer net (7) and the cylindrical inner net (20); the cylindrical inner net (20) and the cylindrical outer net (7) are used for gradient filtration of the mobile phase.

3. The centrifugal column chromatography extraction device according to claim 2, characterized in that: The cylindrical inner net (20) has uniformly arranged 0.22μ mesh holes; the cylindrical outer net (7) has uniformly arranged 0.45μ mesh holes.

4. The centrifugal column chromatography extraction device according to claim 1, characterized in that: The extraction chamber (6) is a cylindrical container, an upper sieve plate (8) is provided between the top of the extraction chamber (6) and the top cover box (9), and a lower sieve plate (5) is provided between the bottom of the extraction chamber (6) and the external connection assembly.

5. The centrifugal column chromatography extraction device according to claim 1, characterized in that: The pipe column (17) is rotatably connected to the external pipe (14) through a connecting module (16); a backup pressure column (21) made of a porous material is provided in the external pipe (14); the backup pressure column (21) is used to increase the backup pressure in the pipe column (17).

6. The centrifugal column chromatography extraction device according to claim 5, characterized in that: The top cover box (9) is provided with an injection tube (10), the upper end of the injection tube (10) is connected to the back pressure column (11) and the connecting tube (12) in sequence, the back pressure column (11) is a filtering column formed of a porous material, and the back pressure column (11) is used to increase the back pressure in the extraction chamber (6); The back pressure in the extraction chamber (6) is less than the backup pressure value in the column (17) to ensure that the flow direction of the chromatographic reagent is unidirectional.

7. The centrifugal column chromatography extraction device according to claim 1, characterized in that: The bearing assembly (18) includes an upper bearing (24) and a lower bearing (25) stacked one above the other, and a sealing ring (9) disposed between the inner rings of the upper bearing (24) and the lower bearing (25). The sealing ring (9) is in close contact with the pipe column (17) via an internal elastic spring structure. The upper bearing (24) and the lower bearing (25) are respectively embedded in the upper bearing buckle box and the lower bearing buckle box, and the upper bearing buckle box and the lower bearing buckle box are connected by buckles.

8. The centrifugal column chromatography extraction device according to claim 1, characterized in that: The external connection assembly comprises a connector (2), an adapter (3) and an external interface (4) connected in sequence, wherein the connector (2) is a hollow structure, the upper end of the connector (2) is sealedly connected to the bottom of the extraction chamber (6), the lower end of the connector (2) is connected to the external interface (4) via the adapter (3), and the external interface (4) is connected to a collector or a detection device.

9. The centrifugal column chromatography extraction device according to claim 1, characterized in that: The invention also includes a rotary drive mechanism; the rotary drive mechanism includes a motor (27), a belt (28) and two pulleys, wherein the motor (27) is arranged on the top cover box (9), and the two pulleys are respectively arranged on the output end of the motor (27) and the pipe column (17), and the two pulleys are connected by the belt (28).

10. A centrifugal column chromatography extraction method using the device according to any one of claims 1 to 9, characterized in that: The following steps are involved: Injecting a sample solution into the chromatographic column (1) through the tubing column (17); The column (17) drives the chromatographic column (1) to rotate; Injecting the extraction liquid into the extraction chamber (6) to maintain the back pressure value in the extraction chamber (6) smaller than the back pressure value in the column (17); The separated components are collected at the bottom of the extraction chamber (6).

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