Micro-fluidic chip and preparation method thereof

Abstract

The invention provides a micro-fluidic chip and a preparation method thereof.The micro-fluidic chip comprises a first fluid inlet, a second fluid inlet, a reaction treatment unit and a sample collection port, and the reaction treatment unit comprises a plurality of reaction channels connected in parallel; each reaction channel is communicated with the first fluid inlet and the second fluid inlet through the first fluid channel; and each reaction channel is communicated with the sample collection port through a second fluid channel. According to the micro-fluidic chip and the preparation method thereof provided by the embodiment of the invention, the fluid treatment efficiency and the overall productivity are improved.

Description

Technical Field

[0001] The present invention relates to the field of micro-electromechanical technology, and in particular to a microfluidic chip and a preparation method thereof. Background Art

[0002] Microfluidic chips can manipulate and process microfluidics at the micrometer scale. By constructing microchannel networks, they enable precise control, mixing, reaction, separation, and detection of fluids. They are widely used in biology, chemistry, medicine, environmental monitoring, optics, and other fields.

[0003] A commercial microfluidic chip has been developed that includes a mixing channel for mixing different fluids. This channel is designed to be curved and has multiple serially connected annular structures to enhance mixing. However, the curvature of the channel and the obstruction of the annular structures reduce the flow rate, limiting throughput and fluid processing capacity. Therefore, developing a microfluidic chip that can improve fluid processing efficiency has become a critical issue in this field. Summary of the Invention

[0004] In response to the problems in the prior art, embodiments of the present invention provide a microfluidic chip and a method for preparing the same, which can at least partially solve the problems in the prior art.

[0005] In a first aspect, the present invention provides a microfluidic chip comprising a first fluid inlet, a second fluid inlet, a reaction processing unit, and a sample collection port, wherein:

[0006] The reaction processing unit includes a plurality of parallel reaction channels, each reaction channel is connected to the first fluid inlet and the second fluid inlet through a first fluid channel;

[0007] Each reaction channel is connected to the sample collection port through a second fluid channel.

[0008] Furthermore, a counter-strengthening structure is provided in the first fluid channel.

[0009] Furthermore, a counter-strengthening structure is provided on one side of the first fluid channel close to the first fluid inlet and the second fluid inlet.

[0010] Furthermore, a counter-strengthening structure is provided on a side of the first fluid channel close to the reaction processing unit.

[0011] Furthermore, a first counter-strengthening structure is provided on a side of the first fluid channel close to the reaction processing unit, and a second counter-strengthening structure is provided on a side of the first fluid channel close to the first fluid inlet and the second fluid inlet.

[0012] Furthermore, a gap between the first hedging reinforcement structure and the second hedging reinforcement structure is greater than 70 micrometers and less than 140 micrometers.

[0013] Furthermore, the hedging reinforcement structure is a circular structure, an elliptical structure or a teardrop-shaped structure.

[0014] Furthermore, the ratio of the diameter of the circular structure to the length of the first fluid channel ranges from 1:2.5 to 1:2.

[0015] Furthermore, the number of the hedging reinforcement structures is 1 or 2.

[0016] Furthermore, a ratio of the length to the width of the first fluid channel ranges from 1.4:1 to 1.6:1.

[0017] Furthermore, the reaction processing unit includes two parallel reaction channels, and the two parallel reaction channels are symmetrically arranged with respect to the first fluid channel.

[0018] In a second aspect, the present application proposes a method for preparing a microfluidic chip, which is used to prepare the microfluidic chip described in any of the above embodiments, comprising:

[0019] preparing a master having a protruding structure on a substrate, wherein the protruding structure corresponds to the microfluidic chip;

[0020] preparing a polydimethylsiloxane layer based on the master;

[0021] cutting and punching the polydimethylsiloxane layer;

[0022] Cleaning the polydimethylsiloxane layer after cutting and punching;

[0023] bonding the cleaned polydimethylsiloxane layer to the base layer;

[0024] The inner surfaces of the bonded polydimethylsiloxane layer and the base layer are subjected to surface modification treatment.

[0025] The microfluidic chip and preparation method thereof provided in an embodiment of the present invention include a first fluid inlet, a second fluid inlet, a reaction processing unit and a sample collection port. The reaction processing unit includes multiple parallel reaction channels, each reaction channel is connected to the first fluid inlet and the second fluid inlet through a first fluid channel; each reaction channel is connected to the sample collection port through a second fluid channel. Due to the provision of multiple parallel reaction channels, the fluid processing efficiency and overall production capacity are improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative work. In the drawings:

[0027] Figure 1 Schematic diagram of the structure of the microfluidic chip provided by the first embodiment of the present invention.

[0028] Figure 2 Schematic diagram of the structure of the microfluidic chip provided by the second embodiment of the present invention.

[0029] Figure 3 Schematic diagram of the structure of the microfluidic chip provided in the third embodiment of the present invention.

[0030] Figure 4 Schematic diagram of the structure of the microfluidic chip provided by the fourth embodiment of the present invention.

[0031] Figure 5 Schematic diagram of the structure of the microfluidic chip provided in the fifth embodiment of the present invention.

[0032] Figure 6 4 is a flow chart of a method for preparing a microfluidic chip provided in accordance with the sixth embodiment of the present invention. DETAILED DESCRIPTION

[0033] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the embodiments of the present invention are further described in detail with reference to the accompanying drawings. Here, the schematic embodiments of the present invention and their descriptions are used to explain the present invention, but are not intended to limit the present invention. It should be noted that, in the absence of conflict, the embodiments in this application and the features in the embodiments can be combined with each other in any way. The acquisition, storage, use, processing, etc. of data in the technical solutions in this application comply with the relevant provisions of laws and regulations. The user information in the embodiments of this application is obtained through legal and compliant channels, and the acquisition, storage, use, processing, etc. of user information are agreed to by the customer.

[0034] In order to facilitate understanding of the technical solution provided by this application, the relevant contents of the technical solution of this application are first explained below.

[0035] Existing commercial microfluidic chips have certain limitations in terms of sample output and fluid processing capabilities, and cannot meet the actual application requirements of high output and high efficiency. In response to the above problems, this application effectively improves the fluid processing capacity and output of microfluidic chips through parallel design and optimization of hedge reinforcement structure. The microfluidic chip structure of this application is particularly suitable for a variety of microfluidic application scenarios involving the reaction or mixing of organic phases and aqueous phases. It has broad application potential and can meet the needs of efficient, rapid and high-yield microfluidic experiments.

[0036] Figure 1 Schematic diagram of the structure of the microfluidic chip provided by the first embodiment of the present invention. Figure 1 As shown, the microfluidic chip provided by the embodiment of the present invention includes a first fluid inlet 1, a second fluid inlet 2, a reaction processing unit 3 and a sample collection port 4, wherein:

[0037] The reaction processing unit 3 includes a plurality of parallel reaction channels 3-1, each reaction channel 3-1 is connected to the first fluid inlet 1 and the second fluid inlet 2 through the first fluid channel 5;

[0038] Each reaction channel 3 - 1 is connected to the sample collection port 4 through the second fluid channel 6 .

[0039] Specifically, the first fluid inlet 1 and the second fluid inlet 2 allow different fluids to flow into the microfluidic chip. For example, the first fluid inlet 1 flows into an organic phase, and the second fluid inlet flows into an aqueous phase; the first fluid inlet 1 flows into an aqueous phase, and the second fluid inlet flows into an organic phase.

[0040] The reaction processing unit 3 includes multiple parallel reaction channels 3-1. Each reaction channel 3-1 is connected to the first fluid inlet 1 via a first fluid channel 5. Fluids flowing from the first fluid inlet 1 can flow into the reaction channel 3-1 through the first fluid channel. Each reaction channel 3-1 is connected to the second fluid inlet 2 via a first fluid channel 5. Fluids flowing from the second fluid inlet 2 can flow into the reaction channel 3-1 through the first fluid channel. Different fluids flowing into the first fluid channel 5 are offset first.

[0041] The reaction processing unit 3 includes a plurality of parallel reaction channels 3-1, each of which is used to achieve mixing and reaction of different fluids. By increasing the reaction channels 3-1, the processing efficiency and overall output of the fluid are improved. A plurality of mixing structures 3-1-1 are provided in each reaction channel 3-1. When the fluid flows through the mixing structure 3-1-1, Dean vortices are easily formed, which is conducive to the mixing and reaction of the fluid. The mixing structure 3-1-1 can be an annular structure, an elliptical structure or other irregular shapes, and is provided according to actual needs. The embodiment of the present invention does not limit it.

[0042] The fluids mixed in the reaction channels 3 - 1 flow to the sample collection port 4 through the second fluid channel 6 , and then flow out of the microfluidic chip through the sample collection port 4 .

[0043] The microfluidic chip provided in an embodiment of the present invention includes a first fluid inlet, a second fluid inlet, a reaction processing unit and a sample collection port. The reaction processing unit includes multiple parallel reaction channels, each reaction channel is connected to the first fluid inlet and the second fluid inlet through a first fluid channel; each reaction channel is connected to the sample collection port through a second fluid channel. Due to the provision of multiple parallel reaction channels, the fluid processing efficiency and overall production capacity are improved.

[0044] Figure 2 Schematic diagram of the structure of the microfluidic chip provided by the second embodiment of the present invention. Figure 2 As shown, based on the above embodiments, a counter-strengthening structure 7 is further provided in the first fluid channel 5. The specific number of the counter-strengthening structures 7 is set according to actual needs and is not limited in the embodiment of the present invention.

[0045] The counter-reinforcement structure effectively enhances the initial counter-reaction between the different fluids, promoting rapid mixing and reaction between the two phases and improving reaction efficiency. Furthermore, the counter-reinforcement structure improves the uniformity of the mixed fluid and achieves a lower Polymer Dispersion Index (PDI) value.

[0046] For example, Figure 2 As shown, a counter-strengthening structure 7 is provided in the first fluid channel 5 .

[0047] For example, Figure 4 As shown, two counter-strengthening structures 7 are provided in the first fluid channel 5 .

[0048] like Figure 2 As shown, on the basis of the above embodiments, further, a counter-strengthening structure 7 is provided on one side of the first fluid channel 5 close to the first fluid inlet 1 and the second fluid inlet 2 .

[0049] Figure 3 Schematic diagram of the structure of the microfluidic chip provided by the third embodiment of the present invention. Figure 3 As shown, a counter-strengthening structure 7 is provided on one side of the first fluid channel 5 close to the reaction processing unit 3 .

[0050] Figure 4 Schematic diagram of the structure of the microfluidic chip provided by the fourth embodiment of the present invention. Figure 4As shown, on the basis of the above embodiments, a first counter-strengthening structure 7-1 is further provided on the side of the first fluid channel 5 close to the reaction treatment unit 3, and a second counter-strengthening structure 7-2 is provided on the side of the first fluid channel 5 close to the first fluid inlet 1 and the second fluid inlet 2.

[0051] In addition to the above embodiments, the gap between the first and second hedging reinforcement structures 7-1, 7-2 is greater than 70 microns and less than 140 microns. This gap between the first and second hedging reinforcement structures 7-1, 7-2 effectively promotes fluid counteraction and mixing, prevents fluid blockage, and regulates flow rate and pressure distribution, thereby improving the preparation quality of nanoformulations and the overall performance of the system.

[0052] Based on the above embodiments, the hedging reinforcement structure 7 is further configured as a circular structure, an elliptical structure, or a teardrop-shaped structure. A circular structure means that the cross section of the hedging reinforcement structure 7 is circular; an elliptical structure means that the cross section of the hedging reinforcement structure 7 is elliptical; and a teardrop-shaped structure means that the cross section of the hedging reinforcement structure 7 is teardrop-shaped.

[0053] On the basis of the above embodiments, further, the ratio of the diameter of the circular structure to the length of the first fluid channel 5 is in the range of 1:2.5 to 1:2. For example, the ratio of the diameter of the circular structure to the length of the first fluid channel 5 is 1:2.35. If the diameter of the circular structure is too large, the flow rate of the fluid in the first fluid channel will be reduced. If the diameter of the circular structure is too small, the hedging effect will be limited. Setting the ratio of the diameter of the circular structure to the length of the first fluid channel 5 to between 1:2.5 and 1:2 can bring about a better hedging effect at a certain flow rate. In addition, if the circular structure is too large, it will also cause excessive fluid pressure, which can easily damage the chip.

[0054] On the basis of the above embodiments, further, the number of the hedging reinforcement structures 7 is 1 or 2.

[0055] On the basis of the above embodiments, further, the length and width ratio of the first fluid channel 5 is in the range of 1.4:1 to 1.6:1. For example, the length and width ratio of the first fluid channel 5 is 1.5:1. The length and width ratio of the first fluid channel 5 is in the range of 1.4:1 to 1.6:1, which aims to achieve the best balance between fluid flow characteristics and mixing effect. Specifically, the channel structure of the aspect ratio of the first fluid channel 5 in the above value range can generate moderate shear force during the fluid flow process, effectively promote the mixing of the two fluids, thereby improving the mixing uniformity and reaction efficiency. In contrast, when the channel aspect ratio approaches 1:1, the fluid flow shows a strong symmetry, the shear effect is weakened, resulting in reduced mixing efficiency and uneven mixing. On the other hand, if the aspect ratio is significantly greater than 1.6:1, the fluid may generate a higher pressure difference at the intersection, which can easily cause fluid separation or unstable flow, which is not conducive to maintaining the mixing effect. Therefore, the design of the length and width ratio of the first fluid channel 5 in the range of 1.4:1 to 1.6:1 can not only ensure appropriate shearing between fluids, but also avoid the negative impact of excessive pressure differences on flow stability, thereby optimizing fluid mixing efficiency and improving the preparation quality of nanoformulations.

[0056] like Figure 1 As shown, based on the above embodiments, the reaction processing unit 3 further includes two parallel reaction channels 3 - 1 , and the two parallel reaction channels 3 - 1 are symmetrically arranged about the first fluid channel 5 .

[0057] Figure 5 Schematic diagram of the structure of the microfluidic chip provided by the fifth embodiment of the present invention. Figure 5 As shown, based on the above embodiments, the reaction processing unit 3 further includes three parallel reaction channels 3-1, each reaction channel 3-1 is connected to the first fluid channel 5, and each reaction channel 3-1 is connected to the second fluid channel 6.

[0058] Figure 6 FIG. 1 is a flow chart of a method for preparing a microfluidic chip according to a sixth embodiment of the present invention. Figure 6 As shown, the method for preparing a microfluidic chip provided in an embodiment of the present invention is used to prepare the microfluidic chip described in any of the above embodiments, comprising:

[0059] S601, preparing a master having a protruding structure on a substrate, wherein the protruding structure corresponds to the microfluidic chip;

[0060] Specifically, a secondary exposure technique is used to etch a raised structure corresponding to the microfluidic chip on the surface of the substrate, and the etched substrate is used as a master. It is understandable that the raised structure corresponds to the structure through which the fluid flows in the first fluid inlet, the second fluid inlet, the first fluid channel, the second fluid channel, and the reaction channel of the microfluidic chip. The height of the raised structure of the master is set according to actual needs. The secondary exposure technique can form high-precision and complex microstructures on the surface of the substrate to ensure the accuracy and stability of the flow channel in the subsequent preparation process. The substrate can be a silicon wafer, a glass sheet, etc., which can be selected according to actual needs, and the embodiment of the present invention does not limit it.

[0061] S602, preparing a polydimethylsiloxane layer based on the master;

[0062] Specifically, liquid polydimethylsiloxane (PDMS) is poured onto the master, dried and solidified, and then the solidified PDMS is peeled off from the master to form a PDMS layer. It is understood that recessed structures corresponding to the raised structures of the master are formed on the PDMS layer.

[0063] Among them, the liquid PDMS is obtained by mixing glue A and glue B in a mass ratio of 12:1, and stirring evenly to fully mix glue A and glue B. After the liquid PDMS is poured onto the master, a vacuum dryer can be used to perform a vacuum operation to remove bubbles generated during the mixing process of glue A and glue B, thereby avoiding the adverse effects of bubbles on the structure of the microfluidic chip. Glue A and glue B are selected according to actual needs, and the embodiments of the present invention do not limit this. For example, glue A uses polymethyl methacrylate (PMMA) and glue B uses polystyrene (PS).

[0064] The master plate with the liquid PDMS cast can be placed in a 70°C oven and baked for more than 2 hours until the liquid PDMS is completely cured. The above process forms a stable three-dimensional microstructure in the solid state of PDMS through a thermal curing reaction.

[0065] S603, cutting and punching the polydimethylsiloxane layer;

[0066] Specifically, the polydimethylsiloxane layer is cut and perforated, specifically the recessed structures formed on the polydimethylsiloxane layer corresponding to the raised structures of the master. This perforation is performed to create a first fluid inlet, a second fluid inlet, and a sample collection port. Due to the small size of microfluidic chips, they are typically produced in batches. Cutting is performed to separate the polydimethylsiloxane layer corresponding to a single microfluidic chip.

[0067] S604, cleaning the polydimethylsiloxane layer after cutting and punching;

[0068] Specifically, the cut and punched PDMS layer is placed in a plasma cleaner and treated with oxygen plasma to remove organic contaminants from the PDMS layer's surface and improve its surface hydrophilicity. To ensure uniform surface treatment of the PDMS layer, the cut and punched PDMS layer can be repeatedly cleaned.

[0069] S605, bonding the cleaned polydimethylsiloxane layer to the base layer;

[0070] Specifically, the cleaned polydimethylsiloxane layer is precisely aligned with the base layer and then bonded. The bonding can be performed by a hot press bonding method at a preset temperature, with continuous heating for more than 2 hours to ensure a strong bond between the PDMS layer and the base layer. The preset temperature can be 170°C. The above bonding step enhances the stability of the bonding interface between the PDMS layer and the base layer through physical and chemical forces, ensuring the integrity and sealing of the microfluidic chip structure.

[0071] S606: performing surface modification treatment on the inner surfaces of the bonded polydimethylsiloxane layer and the base layer.

[0072] Specifically, a modifier is injected into the bonded polydimethylsiloxane layer and the substrate layer to perform surface modification. After the surface modification is completed, a microfluidic chip can be obtained. The modifier can be injected through the first fluid inlet and the second fluid inlet using a syringe.

[0073] Surface modification helps optimize the interfacial properties of fluids within the microfluidic chip, enhancing the interaction between the fluid and the chip walls, and further improving the precision of fluid manipulation. After surface modification, the microfluidic chip is dried in a 75°C oven to remove volatile solvents from the modifier and ensure the stability of the modified layer within the chip.

[0074] The following describes the use of the microfluidic chip provided in the embodiments of the present invention.

[0075] The microfluidic chip was mounted on a platform designed specifically for nanoformulation microfluidics. The connectors for the first and second fluid inlets, 1 and 2, were connected to the catheters via plastic hoses. The first inlet, 1, served as the organic phase port, while the second inlet, 2, served as the aqueous phase port, ensuring smooth introduction of the different phases. A pressure pump was used to inject the organic and aqueous samples, ensuring uniform flow into the microfluidic chip.

[0076] The organic and aqueous samples flow simultaneously into the microfluidic chip from the first and second fluid inlets 1 and 2, respectively, and into the first fluid channel 5, where they mix and react. The mixed organic and aqueous samples then flow into two or more parallel reaction channels 3-1 of the reaction processing unit 3, where reactions occur separately in each reaction channel 3-1. The resulting mixed sample flows through the second fluid channel 6 into the sample collection port 4, where it exits the microfluidic chip.

[0077] After the organic and aqueous samples flow into the microfluidic chip, a laser pointer is used for preliminary inspection to observe whether the Tyndall effect occurs to determine whether the colloid has been successfully formed. If the Tyndall effect is not observed, it indicates that the colloid has not been produced. If the Tyndall effect is detected, it indicates that the colloid has been successfully generated. At this time, dynamic light scattering technology is used to analyze the nanoformulation to determine the particle size and dispersion index (PDI) to evaluate the quality and stability of the formulation. Based on the dynamic light scattering results, if the particle size or dispersion index is found to not meet the expected standards, the preparation conditions can be adjusted and optimized to ensure that the performance of the final product meets the relevant requirements.

[0078] The performance of the microfluidic chip provided by the embodiment of the present invention is verified and illustrated by an example below.

[0079] The embodiment of the present invention verifies the structural performance of the microfluidic chip provided by the embodiment of the present invention by applying a mature nano-preparation formula containing soy lecithin, cholesterol and sodium cholate. First, the ingredients of the above-mentioned nano-preparation formula are weighed in a predetermined proportion and dissolved in ethanol to prepare a lipid mixture with a concentration of 10 mg / mL. Then, the syringe pump is started and connected to the microfluidic chip to ensure the accurate delivery of the fluid. A 1mL syringe is used to draw about 1mL of the lipid mixture as an organic phase sample, and a 10mL syringe is used to draw about 10mL of pure water as an aqueous phase sample. Two syringes are fixed in the syringe pump, the total flow rate is set to 4mL / min, and the flow rate ratio of the organic phase sample to the aqueous phase sample is adjusted to 1:9.

[0080] During the experiment, in order to ensure the acquisition of high-quality nano preparations, the mixed samples of the first 2-3 seconds and the last 1 second of the sample collection port were discarded, and the mixed samples of the middle section were collected for analysis. According to the dynamic light scattering (DLS) analysis results, compared with the commercial chip in the prior art, the nano preparation particle size prepared by the microfluidic chip provided in the embodiment of the present invention has no significant difference, all within the range of 46.5nm to 50.4nm. In addition, the microfluidic chip provided in the embodiment of the present invention has no significant difference in polymer dispersion index (PDI) from the commercial chip in the prior art, both being 0.175, indicating that the microfluidic chip provided in the embodiment of the present invention has no significant effect on particle size distribution.

[0081] Notably, the microfluidic chip provided by the present invention significantly optimizes the initial counterbalancing effect between the organic and aqueous phases by introducing a circular structure as a counterbalancing reinforcement, thereby enhancing the rapid mixing and reaction of the two phases. This optimization significantly reduces the PDI of the microfluidic chip with the circular structure to between 0.119 and 0.135, indicating that the introduction of the circular structure effectively improves the uniformity and stability of the nanoformulation.

[0082] The experimental results of the embodiments of the present invention verify the high efficiency of the microfluidic chip provided by the embodiments of the present invention in the preparation of nanoformulations. In particular, through the optimization of the parallel design and circular structure, the stability and uniformity of the nanoformulations are significantly improved, and it has broad application prospects and technical advantages.

[0083] The microfluidic chip provided by the embodiment of the present invention significantly improves the fluid processing capacity and sample yield of the chip through the dual optimization of parallel design and hedging reinforcement structure, and solves the limitations of existing commercial microfluidic chips in high-yield and high-efficiency applications. Through the parallel design, multiple parallel reaction units are set in the microfluidic chip, which can process more fluid samples without increasing the volume of the chip, thereby effectively improving the yield and fluid processing efficiency. In addition, the introduction of the hedging reinforcement structure further optimizes the initial hedging effect between the organic phase and the aqueous phase, enhances the rapid mixing and reaction of the two phases, and improves the reaction rate and product quality. Through the eddy effect and shear force in fluid dynamics, the circular structure can accelerate the formation of the reaction interface, thereby improving the reaction efficiency and product consistency. Overall, the microfluidic chip provided by the present invention has higher production efficiency and better reaction performance, and is particularly suitable for microfluidic application scenarios with efficient preparation, rapid reaction and high yield. The technical solution of the present application not only expands the application scope of the microfluidic chip, but also has broad market potential, and can meet the requirements of modern microfluidic experiments and production for efficiency, precision and scalability.

[0084] Throughout this specification, reference to terms such as "one embodiment," "a specific embodiment," "some embodiments," "for example," "example," "specific example," or "some examples" means that the specific features, structures, materials, or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

[0085] The specific embodiments described above further illustrate the objectives, technical solutions and beneficial effects of the present invention in detail. It should be understood that the above description is only a specific embodiment of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A microfluidic chip, characterized in that: It includes a first fluid inlet, a second fluid inlet, a reaction processing unit and a sample collection port, wherein: The reaction processing unit includes a plurality of parallel reaction channels, each reaction channel is connected to the first fluid inlet and the second fluid inlet through a first fluid channel; Each reaction channel is connected to the sample collection port through a second fluid channel.

2. The microfluidic chip according to claim 1, characterized in that A counter-strengthening structure is provided in the first fluid channel.

3. The microfluidic chip according to claim 2, characterized in that: A counter-strengthening structure is provided on one side of the first fluid channel close to the first fluid inlet and the second fluid inlet.

4. The microfluidic chip according to claim 2, characterized in that A counter-strengthening structure is provided on a side of the first fluid channel close to the reaction processing unit.

5. The microfluidic chip according to claim 2, characterized in that: A first counter-strengthening structure is provided on a side of the first fluid channel close to the reaction processing unit, and a second counter-strengthening structure is provided on a side of the first fluid channel close to the first fluid inlet and the second fluid inlet.

6. The microfluidic chip according to claim 5, characterized in that: A gap between the first impact reinforcement structure and the second impact reinforcement structure is greater than 70 micrometers and less than 140 micrometers.

7. The microfluidic chip according to claim 2, characterized in that: The hedging reinforcement structure is a circular structure, an elliptical structure or a teardrop-shaped structure.

8. The microfluidic chip according to claim 7, characterized in that: The ratio of the diameter of the circular structure to the length of the first fluid channel ranges from 1:2.5 to 1:

2.

9. The microfluidic chip according to claim 2, characterized in that: The number of the hedging reinforcement structures is 1 or 2.

10. The microfluidic chip according to claim 1, characterized in that: The ratio of the length to the width of the first fluid channel ranges from 1.4:1 to 1.6:

1.

11. The microfluidic chip according to claim 1, characterized in that: The reaction processing unit includes two parallel reaction channels, and the two parallel reaction channels are symmetrically arranged with respect to the first fluid channel.

12. A method for preparing a microfluidic chip, characterized in that: For preparing the microfluidic chip according to any one of claims 1 to 11, comprising: preparing a master having a protruding structure on a substrate, wherein the protruding structure corresponds to the microfluidic chip; preparing a polydimethylsiloxane layer based on the master; cutting and punching the polydimethylsiloxane layer; Cleaning the polydimethylsiloxane layer after cutting and punching; bonding the cleaned polydimethylsiloxane layer to the base layer; The inner surfaces of the bonded polydimethylsiloxane layer and the base layer are subjected to surface modification treatment.

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