Three-dimensional paper-based rotary microfluidic device

Abstract

The invention relates to the technical field of microfluidics, in particular to a three-dimensional paper-based rotating microfluidics device which comprises a first functional layer, a second functional layer and a third functional layer which are sequentially laminated and compounded from top to bottom, a rotating shaft is arranged on the upper surface of the third functional layer, a first axis hole allowing rotation along the rotating shaft is formed in the first functional layer, and a second axis hole allowing rotation along the rotating shaft is formed in the second functional layer. The second functional layer is provided with a second axis hole allowing rotation along the rotating shaft; the first functional layer is provided with a sample introduction hole and an adsorption block at the bottom; the second functional layer is provided with a sample receiving groove matched with the sample injection hole and a sample separation and enrichment hole matched with the adsorption block, the sample receiving groove is connected with the sample separation and enrichment hole through a sample flowing groove, and the sample separation and enrichment hole is provided with a paper-based bearing sheet for separating a sample in a crossed manner; the third functional layer is provided with a sample analysis block matched with the sample separation and enrichment hole. The device can be used for simultaneously carrying out the steps of sample introduction, impurity analysis, flow division, separation and enrichment, detection and the like on a plurality of samples.

Description

Technical Field

[0001] The present invention relates to the field of microfluidic technology, in particular to a three-dimensional paper-based rotating microfluidic device. Background Art

[0002] In recent years, food safety has become a major concern and a major concern for people's well-being. Edible oil, a necessity, has seen increasing demand year after year. Currently, China's edible oil consumption ranks first globally, making it crucial to ensure the safety and quality of edible oil. Edible oil is susceptible to contamination and deterioration during production and cooking. Benzo[a]pyrene (B[a]P), a potent carcinogen, is easily produced during this process. It can accumulate in the human body over long periods of time, leading to death and seriously impacting people's health and safety. Commonly used methods for detecting BaP include high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry, fluorescence spectroscopy, and Raman scattering (SERS). These traditional methods often require expensive equipment, complex and time-consuming detection steps, and consume large amounts of chemical reagents. Therefore, developing novel detection methods to overcome these shortcomings is a hot topic and a challenge in current research.

[0003] Microfluidics paper-based analytical devices (μPADs). In 2007, Martinez et al. first proposed a paper-based microfluidic chip. They prepared closed hydrophobic boundaries on paper to form microfluidic channels, and used capillary action to transport samples to the detection area for reaction with the substrate. Subsequently, they prepared the first 3D paper chip for rapid detection of target analytes. Paper as a substrate material has many advantages: (1) Paper is widely available and low cost; (2) It is light and thin, making it easy to store and transport; (3) Paper is easy to process and modify, and operations such as printing, coating, modification, and cutting are very convenient; (4) Paper is biodegradable and environmentally friendly; (5) It has a certain mechanical strength and can be formed into a support structure with multi-layer channels through shaping and stacking; (6) The capillary effect of paper allows liquid to flow automatically without the need for external pumps, which also avoids the bubble problem common in traditional microfluidic systems.

[0004] Therefore, the research on paper-based microfluidic analytical devices (μPADs) in the field of food testing is increasing. They have the potential to become a replacement for traditional microfluidic chips in applications such as food quality monitoring. It is crucial to provide a paper-based microfluidic device that has high applicability, simple processing and low processing cost. Summary of the Invention

[0005] In order to solve the above problems, the purpose of the present invention is to provide a three-dimensional paper-based rotating microfluidic device, which aims to improve the production efficiency of microfluidic chips and reduce their production costs. It can carry the various functional structures of the microfluidic device on different functional layers through a layered structure. At the same time, each functional layer has the functions of sample addition, adsorption, sample reception, sample flow, sample separation and enrichment, and sample analysis. It has the characteristics of high applicability, simple processing, low processing cost, and the ability to analyze different samples simultaneously.

[0006] The purpose of the present invention can be achieved by the following technical solutions:

[0007] The present invention provides a three-dimensional paper-based rotating microfluidic device, which cooperates with an external Raman spectrometer when in use. The device comprises a first functional layer, a second functional layer, and a third functional layer, which are laminated and composited in sequence from top to bottom. The upper surface of the third functional layer is provided with a rotating axis. The first functional layer is provided with a first axial center hole allowing rotation along the rotating axis. The second functional layer is provided with a second axial center hole allowing rotation along the rotating axis.

[0008] The first functional layer is provided with a sample injection hole, an adsorption block is provided at the bottom of the first functional layer, and adsorption paper (for example, filter paper with both hydrophobicity and oil absorption) is provided on the surface of the adsorption block; the second functional layer is provided with a sample receiving groove adapted to the sample injection hole, and a sample separation and enrichment well adapted to the adsorption block, the sample receiving groove is connected to the sample separation and enrichment well through a sample flow groove, and a paper-based carrier sheet for separating samples is provided at the intersection of the sample separation and enrichment well, and filter paper (for example, hydrophilic filter paper) is provided on the surface of the sample receiving groove, the sample flow groove, and the sample separation and enrichment well; the third functional layer is provided with a sample analysis block adapted to the sample separation and enrichment well, and filter paper (for example, hydrophilic filter paper) is provided on the surface of the sample analysis block;

[0009] Among them, the adsorption block and the adsorption paper are used to adsorb impurities remaining after the sample is separated and enriched by the sample separation and enrichment holes; the sample analysis block and the filter paper are used together with an external Raman spectrometer to analyze the components after the sample is separated and enriched by the sample separation and enrichment holes and the filter paper.

[0010] In one embodiment of the present invention, the first axial hole is provided at the center of the first functional layer;

[0011] The injection holes are arranged in a plurality along the circumference of the first axial hole. The adsorption blocks are arranged on a side of the injection holes away from the first axial hole and are symmetrically arranged along the circumference of the first axial hole.

[0012] Preferably, the injection hole is a fan-shaped injection hole symmetrically arranged along the first axial hole, and the adsorption block is a circular adsorption block symmetrically arranged along the first axial hole of the salt; the sample receiving groove is a circular sample receiving hole, the sample flow groove is a rectangular sample flow groove, and the sample separation and enrichment hole is a circular sample separation and enrichment hole; the sample receiving groove and the sample analysis block are hydrophilic areas; the edge dimensions of the sample analysis block and the adsorption block are the same.

[0013] In one embodiment of the present invention, the second axial hole is provided at the center of the second functional layer;

[0014] The size of the sample receiving groove is smaller than that of the injection hole;

[0015] The paper-based carrier sheet is in the shape of a thin long strip.

[0016] In one embodiment of the present invention, the sample flow channel is arranged on a side of the sample receiving channel away from the second axial hole; the sample separation and enrichment hole is arranged on a side of the sample flow channel away from the second axial hole;

[0017] Each group of sample receiving slots is provided with a plurality of sample flow slots and sample separation and enrichment wells connected to the plurality of sample flow slots;

[0018] The lower surface of the second functional layer is provided with a concentric groove which allows relative movement with the sample analysis block.

[0019] In one embodiment of the present invention, the width of the concentric groove is the maximum outer diameter of the sample separation and enrichment hole.

[0020] In one embodiment of the present invention, the size of the sample analysis block is smaller than the size of the sample separation and enrichment well.

[0021] In one embodiment of the present invention, the depth of the sample flow channel gradually increases along the direction of the sample separation and enrichment hole of the sample receiving channel.

[0022] In one embodiment of the present invention, the end portion of the first functional layer is provided with a first auxiliary moving block that allows the first functional layer to be rotated;

[0023] The end of the second functional layer is provided with a second auxiliary moving block that allows the second functional layer to be rotated.

[0024] In one embodiment of the present invention, the thickness of the adsorption block is the same as the height from the upper surface of the sample separation and enrichment hole to the upper surface of the second functional layer, so as to ensure that the lower surface of the adsorption block can fit closely with the upper surface of the sample separation and enrichment hole.

[0025] In one embodiment of the present invention, the thickness of the sample analysis block is the same as the height from the lower surface of the sample separation and enrichment well to the lower surface of the second functional layer, so as to ensure that the upper surface of the sample analysis block can fit tightly with the lower surface of the sample separation and enrichment well.

[0026] Compared with the prior art, the present invention has the following beneficial effects:

[0027] (1) The present invention provides a three-dimensional paper-based rotating microfluidic device, which adopts a layered and stacked construction method to divide the microfluidic device into three functional layers. The first functional layer has a detection sample injection area (injection hole) and an impurity adsorption area (adsorption block). The second functional layer has a detection sample receiving area (sample receiving slot), a sample flow slot and a sample separation and enrichment hole, and a concentric groove that allows relative movement with the sample analysis block. The third functional layer has a sample analysis area (sample analysis block) for sample detection. The various functional layers are connected by rotatable concentric axes.

[0028] (2) The present invention provides a three-dimensional paper-based rotating microfluidic device, in which the functional structures between the various functional layers can be completed by separate processing, and the adsorption block and the sample analysis block can be adjusted in height and thickness as required, so that different fitting effects can be achieved. The inclination angle of the sample flow channel can also be adjusted according to the required sample flow rate, so that microchannels with different flow rate specifications can be achieved. The pore size of the paper selected for the sample receiving slot and the sample separation and enrichment hole can be adjusted as needed to achieve arbitrary control of the detection flow of the sample; through the synergy of the above-mentioned contents, the purpose of optimizing the detection performance and detection efficiency of the microfluidic device is achieved.

[0029] (3) The three-dimensional paper-based rotating microfluidic device provided by the present invention realizes the coordination between the various functional structural units of the microfluidic device in a layered structure, reduces the probability of deformation during the assembly of the microfluidic device, and can improve the quality level of the microfluidic device. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 1 is a schematic diagram of the overall structure of a three-dimensional paper-based rotating microfluidic device according to Example 1;

[0031] Figure 2 This is a front view of the first functional layer of a three-dimensional paper-based rotational microfluidic device according to Example 1;

[0032] Figure 3 This is a schematic diagram of the reverse side of the first functional layer of a three-dimensional paper-based rotating microfluidic device according to Example 1;

[0033] Figure 4 This is a front view of the second functional layer of a three-dimensional paper-based rotating microfluidic device according to Example 1;

[0034] Figure 5 This is a schematic diagram of the reverse side of the second functional layer of a three-dimensional paper-based rotating microfluidic device according to Example 1;

[0035] Figure 6 This is a front view of the third functional layer of a three-dimensional paper-based rotational microfluidic device according to Example 1;

[0036] Figure 7 The results of the detection of B[a]P solutions with different concentrations using a three-dimensional paper-based rotating microfluidic device and a Raman spectrometer are shown;

[0037] Figure 8 The graphs show the results of B[a]P solutions with different concentrations detected only by Raman spectrometer;

[0038] Figure numerals: 1. First functional layer; 11. First auxiliary movable block; 12. Injection hole; 13. First axial hole; 14. Adsorption block; 2. Second functional layer; 21. Second auxiliary movable block; 22. Second axial hole; 23. Sample receiving groove; 24. Sample flow groove; 25. Sample separation and enrichment hole; 26. Concentric groove; 3. Third functional layer; 31. Rotation axis; 3. Sample analysis block. DETAILED DESCRIPTION

[0039] The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.

[0040] In the description of the present invention, unless otherwise expressly specified or limited, the terms "connected," "connected," and "fixed" should be understood in a broad sense. For example, they may refer to fixed connections, detachable connections, or integration; mechanical connections or electrical connections; direct connections or indirect connections through an intermediate medium; and internal communication between two components or interaction between two components. Those skilled in the art will understand the specific meanings of the above terms in the present invention in specific circumstances.

[0041] In the present invention, unless otherwise expressly specified or limited, a first feature being "above" or "below" a second feature may include the first and second features being in direct contact, or may include the first and second features being in contact not directly but through another feature between them. Furthermore, a first feature being "above," "above," and "above" a second feature may include the first feature being directly above or obliquely above the second feature, or may simply mean that the first feature is higher in level than the second feature. A first feature being "below," "below," and "below" a second feature may include the first feature being directly below or obliquely below the second feature, or may simply mean that the first feature is lower in level than the second feature.

[0042] In the description of this embodiment, terms such as "upper," "lower," "left," and "right" are used to refer to positions or locations based on those shown in the accompanying drawings. These terms are intended solely to facilitate description and simplify operation, and are not intended to indicate or imply that the devices or components referred to must have, be constructed, or operate in a specific orientation. Therefore, they should not be construed as limitations on the present invention. Furthermore, the terms "first" and "second" are used solely for descriptive purposes and have no special meaning.

[0043] In the following embodiments, unless otherwise specified, the structures or components used are conventional structures or components in the art, as long as they can achieve the corresponding functions; unless otherwise specified, the reagents used are commercially available reagents, and the detection means and methods used are conventional detection means and methods in the art.

[0044] Example 1

[0045] This embodiment provides a three-dimensional paper-based rotating microfluidic device, such as Figures 1 to 6 As shown, a three-dimensional paper-based rotating microfluidic device is used in conjunction with an external Raman spectrometer. The device comprises a first functional layer 1, a second functional layer 2, and a third functional layer 3, which are laminated and composited from top to bottom. The third functional layer 3 has a rotating axis 31 on its upper surface. The first functional layer 1 has a first axial hole 13 that allows rotation along the rotating axis 31. The second functional layer 2 has a second axial hole 22 that allows rotation along the rotating axis 31.

[0046] The first functional layer 1 is provided with an injection hole 12, and an adsorption block 14 is provided at the bottom of the first functional layer 1, and an adsorption paper is provided on the surface of the adsorption block 14; the second functional layer 2 is provided with a sample receiving groove 23 adapted to the injection hole 12, and a sample separation and enrichment hole 25 adapted to the adsorption block 14, the sample receiving groove 23 is connected to the sample separation and enrichment hole 25 through a sample flow groove 24, and the sample separation and enrichment hole 25 is cross-arranged with a paper-based carrier sheet for separating samples, and the sample receiving groove 23, the sample flow groove 24 and the sample separation and enrichment hole 25 are provided with filter paper; the third functional layer 3 is provided with a sample analysis block 32 adapted to the sample separation and enrichment hole 25, and the surface of the sample analysis block 32 is provided with filter paper;

[0047] Among them, the adsorption block 14 is used in conjunction with the adsorption paper to adsorb impurities remaining after the sample is separated and enriched by the sample separation and enrichment hole 25; the sample analysis block 32 is used in conjunction with the filter paper and an external Raman spectrometer to analyze the components after the sample is separated and enriched by the sample separation and enrichment hole 25 and the filter paper.

[0048] Furthermore, the first axial hole 13 is arranged at the center position of the first functional layer 1; the injection holes 12 are arranged in a plurality along the circumference of the first axial hole 13, and the adsorption blocks 14 are arranged on the side of the injection hole 12 away from the first axial hole 13, and are arranged symmetrically along the circumference of the first axial hole 13.

[0049] Preferably, the injection hole 12 is a fan-shaped injection hole 12 symmetrically arranged along the first axial hole 13, and the adsorption block 14 is a circular adsorption block 14 symmetrically arranged along the first axial hole 13 of salt; the sample receiving groove 23 is a circular sample receiving hole, the sample flow groove 24 is a rectangular sample flow groove 24, and the sample separation and enrichment hole 25 is a circular sample separation and enrichment hole 25; the sample receiving groove 23 and the sample analysis block 32 are hydrophilic areas; the edge dimensions of the sample analysis block 32 and the adsorption block 14 are the same.

[0050] Furthermore, the second axial hole 22 is provided at the center of the second functional layer 2 ; the size of the sample receiving groove 23 is smaller than that of the injection hole 12 ; and the paper-based carrier sheet is in the shape of a thin long strip.

[0051] Furthermore, the sample flow channel 24 is provided on a side of the sample receiving channel 23 away from the second axial hole 22; the sample separation and enrichment hole 25 is provided on a side of the sample flow channel 24 away from the second axial hole 22;

[0052] Each group of sample receiving slots 23 is provided with a plurality of sample flow slots 24 and sample separation and enrichment holes 25 connected to the plurality of sample flow slots 24;

[0053] The lower surface of the second functional layer 2 is provided with a concentric groove 26 that allows relative movement with the sample analysis block 32 .

[0054] Furthermore, the width of the concentric groove 26 is the maximum outer diameter of the sample separation and enrichment hole 25 .

[0055] Furthermore, the size of the sample analysis block 32 is smaller than the size of the sample separation and enrichment hole 25 , and the size of the adsorption block 14 is smaller than the size of the sample separation and enrichment hole 25 .

[0056] Furthermore, the depth of the sample flow channel 24 gradually increases along the direction from the sample receiving channel 23 to the sample separation and enrichment hole 25 .

[0057] Furthermore, a first auxiliary moving block 11 is provided at the end of the first functional layer 1 to allow the first functional layer 1 to rotate; and a second auxiliary moving block 21 is provided at the end of the second functional layer 2 to allow the second functional layer 2 to rotate.

[0058] Furthermore, the thickness of the adsorption block 14 is the same as the height from the upper surface of the sample separation and enrichment hole 25 to the upper surface of the second functional layer 2 to ensure that the lower surface of the adsorption block 14 can fit tightly with the upper surface of the sample separation and enrichment hole 25 .

[0059] Furthermore, the thickness of the sample analysis block 32 is the same as the height from the lower surface of the sample separation and enrichment well 25 to the lower surface of the second functional layer 2 to ensure that the upper surface of the sample analysis block 32 can fit tightly with the lower surface of the sample separation and enrichment well 25 .

[0060] The three-dimensional paper-based rotating microfluidic device provided in this embodiment can be prepared by the following method:

[0061] (1) Preparation of the first functional layer 1: The first functional layer 1 is obtained by instrument printing. Adsorption paper with different adsorption properties is selected as needed, cut and placed on the lower surface of the adsorption block 14;

[0062] (2) Preparation of the second functional layer 2: The second functional layer 2 (including the cross-arranged paper-based support sheet) is obtained by instrument printing. Whatman filter papers of different pore sizes are selected as needed, cut and placed on the sample receiving slot 23, the sample flow slot 24, and the upper surface of the paper-based support sheet;

[0063] (3) Preparation of the third functional layer 3: The third functional layer 3 is obtained by instrument printing. Whatman filter papers of different pore sizes are selected as needed, cut and placed on the upper surface of the sample analysis block 32.

[0064] The "corresponding" and "adaptable" mean that after the functional layers of the three-dimensional paper-based rotary microfluidic device are stacked and aligned, the projections of the corresponding components (such as the injection hole 12 and the sample receiving groove 23, the adsorption block 14 and the sample separation and enrichment hole 25, the sample separation and enrichment hole 25 and the sample analysis block 32, etc.) overlap in the vertical direction.

[0065] For example: the sample separation and enrichment hole 25 is intended to place a cellulose filter paper preloaded with a molecular imprinting polymer of the target molecule to be detected, and the imprinted polymer cellulose filter paper can specifically capture molecules that match the target molecule in terms of molecular shape, size and functional groups. Among them, the cellulose filter paper preloaded with the imprinted polymer of the target molecule to be detected uses the target molecule as a "mold" to form a three-dimensional structure and functional groups complementary to it in the polymer through chemical or physical means, and then removes the template molecule through physical or chemical methods (such as elution), leaving a hole that matches the shape, size and functional groups of the template molecule. The "hole" mentioned can specifically capture molecules that match the hole, and other molecules are separated, thereby achieving the effect of specific separation and enrichment.

[0066] For example, a cellulose filter paper modified with a signal-enhancing substrate (e.g., Raman signal) is placed in the sample analysis area on the front of the third functional layer 3. The pre-loaded signal-enhancing substrate on the cellulose filter paper can significantly enhance the signal intensity (Raman signal) of the target molecule. This signal can be used by a portable Raman spectrometer to illuminate the sample analysis area of ​​the third functional layer 3 with laser light to quickly obtain molecular vibration information, thereby enabling qualitative and quantitative analysis of the substance.

[0067] Example 2

[0068] This embodiment provides a method for using the three-dimensional paper-based rotating microfluidic device described in Example 1, which is specifically as follows:

[0069] The second functional layer of whatman filter paper was prepared by the following method:

[0070] 0.1 mM BaP (acetonitrile: methanol = 3: 1 (V: V)), pyrene, and anthracene were used as template molecules and mixed with dopamine solution (1: 10, V: V) (buffered in 10 mM Tris-HCl with a pH of 8.5) and stirred at constant temperature for 10 min to obtain a prepolymer solution, which was kept away from light throughout the process; finally, the pretreated Whatman filter paper was immersed in the prepolymer solution and completely soaked, and then ammonium persulfate (APS) was added to a final concentration of 1 mM, sealed and stirred at room temperature in the dark for 12 h, and then the filter paper was removed, rinsed three times with deionized water, and vacuum dried again for 2 h; finally, the eluent (V 甲苯 :V 甲醇 =8:2) The plates were removed until they were no longer detected by UV-vis, resulting in Whatman / PDA-MIP with the template molecule removed.

[0071] The third functional layer of whatman filter paper was prepared by the following method:

[0072] 90 mg of silver nitrate was dissolved in 500 mL of water and boiled. 10 mL of 1% sodium citrate solution was added and the mixture was boiled for 1 h. 20 μL of 0.1 M KCl aqueous solution was then added to 1000 mL of the AgNPs suspension to activate the AgNPs surface. 10 μL of Viologens was then added to a final concentration of 1 × 10 -4 M, and then the solution was thoroughly mixed by ultrasonication, and the previously pretreated Whatman filter paper was immersed in the solution for 1 h. The filter paper was taken out, rinsed with deionized water three times, and vacuum dried for 2 h to obtain Whatman / AgNPs-Viologens.

[0073] The pretreated Whatman filter paper was prepared by the following method:

[0074] First, Whatman filter paper was cut into circles of the required size (r = 0.5 cm) and immersed in anhydrous ethanol for 20 min of ultrasonic cleaning to remove surface impurities. The filter paper was then removed, rinsed three times with deionized water, and dried in a vacuum drying oven at 60°C for 2 h. The dried filter paper was then immersed in 0.1 M NaOH solution and allowed to stand for 5 min. The filter paper was then removed and rinsed with deionized water until neutral (pH test paper detection), then vacuum dried again at 60°C for 1 hour, and stored in a desiccator for later use.

[0075] When used, the liquid sample (concentration of 1×10 -4 g / ml, 1×10 -5 g / ml, 1×10 -6 g / ml, 1×10 -7 g / ml, 1×10 -8 g / ml, 1×10 -9 g / ml, 1×10 -10 g / ml, 1×10 -11 g / ml, 1×10 -12 g / ml, 1×10 -13 g / ml, 1×10 -14 g / ml, 1×10 -15 g / ml, 1×10 -16 g / ml B[a]P solution) is added from the injection hole 12, and the liquid sample enters the sample receiving tank 23 through the injection hole 12, and then enters the sample separation and enrichment well 25 through the sample flow tank 24. After the enrichment of the sample separation and enrichment well 25 is completed, the adsorption block 14 is rotated to the corresponding sample separation and enrichment well 25 by a rotation operation so that the adsorption block 14 and the sample separation and enrichment well 25 are closely fitted to adsorb the enriched impurities, and then the adsorption block 14 is removed by a rotation operation; then the sample separation and enrichment well 25 is rotated to the upper surface of the corresponding sample analysis block 32 by a rotation operation so that the sample analysis block 32 and the sample separation and enrichment well 25 are closely fitted, and finally the sample substance analysis of the sample analysis block 32 is performed using an external portable Raman spectrometer (such as Figure 7 shown).

[0076] Only the Raman spectrometer was used to detect the concentration of 1×10 -5 g / ml, 1×10 -6 g / ml, 1×10 -7 g / ml, 1×10 -8 g / ml, 1×10 -9 g / ml, 1×10 -10 g / ml, 1×10 -11 g / ml, 1×10 -12 g / ml of B[a]P solution, the results are as follows Figure 8shown.

[0077] pass Figure 7 and Figure 8 It can be found that the three-dimensional paper-based rotating microfluidic device provided by the present invention can improve detection efficiency while improving detection sensitivity.

[0078] The above description of the embodiments is intended to facilitate understanding and use of the invention by those skilled in the art. It will be apparent that those skilled in the art can readily make various modifications to these embodiments and apply the general principles described herein to other embodiments without requiring inventive effort. Therefore, the present invention is not limited to the above-described embodiments. Improvements and modifications made by those skilled in the art based on the explanations of the present invention without departing from the scope of the present invention should be within the scope of protection of the present invention.

Claims

1. A three-dimensional paper-based rotating microfluidic device, used in conjunction with an external Raman spectrometer, characterized in that: The invention comprises a first functional layer (1), a second functional layer (2) and a third functional layer (3) which are laminated and compounded in sequence from top to bottom, wherein the upper surface of the third functional layer (3) is provided with a rotation axis (31), the first functional layer (1) is provided with a first axial center hole (13) which allows rotation along the rotation axis (31), and the second functional layer (2) is provided with a second axial center hole (22) which allows rotation along the rotation axis (31); The first functional layer (1) is provided with an injection hole (12), an adsorption block (14) is provided at the bottom of the first functional layer (1), and adsorption paper is provided on the surface of the adsorption block (14); the second functional layer (2) is provided with a sample receiving groove (23) adapted to the injection hole (12), and a sample separation and enrichment hole (25) adapted to the adsorption block (14), the sample receiving groove (23) is connected to the sample separation and enrichment hole (25) through a sample flow groove (24), and the sample separation and enrichment hole (25) is cross-arranged with a paper-based carrier sheet for separating samples, and filter paper is provided on the surface of the sample receiving groove (23), the sample flow groove (24) and the sample separation and enrichment hole (25); the third functional layer (3) is provided with a sample analysis block (32) adapted to the sample separation and enrichment hole (25), and filter paper is provided on the surface of the sample analysis block (32); The adsorption block (14) is used in conjunction with the adsorption paper to adsorb impurities remaining after the sample is separated and enriched by the sample separation and enrichment hole (25); and the sample analysis block (32) is used in conjunction with the filter paper and an external Raman spectrometer to analyze the components after the sample is separated and enriched by the sample separation and enrichment hole (25) and the filter paper.

2. A three-dimensional paper-based rotating microfluidic device according to claim 1, characterized in that: The first axial hole (13) is arranged at the center of the first functional layer (1); The injection holes (12) are arranged in a plurality along the circumference of the first axial hole (13); the adsorption blocks (14) are arranged on a side of the injection hole (12) away from the first axial hole (13), and are arranged in a plurality of symmetrical directions along the circumference of the first axial hole (13).

3. The three-dimensional paper-based rotating microfluidic device according to claim 1, characterized in that: The second axial hole (22) is arranged at the center of the second functional layer (2); The size of the sample receiving groove (23) is smaller than the size of the injection hole (12); The paper-based carrier sheet is in the shape of a thin long strip.

4. A three-dimensional paper-based rotational microfluidic device according to claim 3, characterized in that: The sample flow channel (24) is arranged on a side of the sample receiving channel (23) away from the second axial hole (22); the sample separation and enrichment hole (25) is arranged on a side of the sample flow channel (24) away from the second axial hole (22); Each group of sample receiving slots (23) is provided with a plurality of sample flow slots (24) and sample separation and enrichment holes (25) connected to the plurality of sample flow slots (24); The lower surface of the second functional layer (2) is provided with a concentric groove (26) that allows relative movement with the sample analysis block (32).

5. The three-dimensional paper-based rotating microfluidic device according to claim 4, characterized in that: The width of the concentric groove (26) is the maximum outer diameter of the sample separation and enrichment hole (25).

6. The three-dimensional paper-based rotational microfluidic device according to claim 4, characterized in that: The size of the sample analysis block (32) is smaller than that of the sample separation and enrichment hole (25).

7. The three-dimensional paper-based rotational microfluidic device according to claim 4, characterized in that: The depth of the sample flow channel (24) gradually increases along the direction of the sample separation and enrichment hole (25) of the sample receiving channel (23).

8. The three-dimensional paper-based rotational microfluidic device according to claim 1, characterized in that: A first auxiliary moving block (11) is provided at the end of the first functional layer (1) for allowing the first functional layer (1) to rotate; A second auxiliary moving block (21) is provided at the end of the second functional layer (2) for allowing the second functional layer (2) to rotate.

9. The three-dimensional paper-based rotational microfluidic device according to claim 1, characterized in that: The thickness of the adsorption block (14) is the same as the height of the upper surface of the sample separation and enrichment hole (25) from the upper surface of the second functional layer (2), so as to ensure that the lower surface of the adsorption block (14) can be closely fitted with the upper surface of the sample separation and enrichment hole (25).

10. The three-dimensional paper-based rotational microfluidic device according to claim 1, characterized in that: The thickness of the sample analysis block (32) is the same as the height of the lower surface of the sample separation and enrichment hole (25) from the lower surface of the second functional layer (2), so as to ensure that the upper surface of the sample analysis block (32) can be closely fitted with the lower surface of the sample separation and enrichment hole (25).

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