Liquid chromatograph with automatic cleaning function

Abstract

The invention relates to the technical field of chromatographic instrument cleaning, and provides a liquid chromatograph with an automatic cleaning function. The liquid chromatograph comprises a machine body, a machine shell, a chromatographic column, a guard column and the like, the machine shell is rotationally arranged on the machine body, a liquid reservoir is installed in the machine body and can be covered by the machine shell, and the guard column is arranged on the machine shell. The chromatographic column is detachably mounted at the right position in the machine body, and the protection column is detachably mounted at the right part of the machine body. By arranging the three-way valve sleeve, the three-way valve core and the three-way sleeve, the flow direction of pure water or a cleaning agent is controlled by rotating the three-way valve core, so that a tester can freely control whether the pure water or the cleaning agent flows through a chromatographic column and a guard column or not, the chromatographic column is prevented from being washed by the pure water for a long time, the operation is convenient, the chromatographic column and the guard column do not need to be repeatedly disassembled and assembled, and the test efficiency is improved. The liquid suction needle cylinder is used for sucking pure water or cleaning agents in the original liquid frame, and the dosage of various cleaning agents is accurately controlled through scales on the liquid suction needle cylinder.

Description

Technical Field

[0001] The present invention relates to the technical field of chromatograph cleaning, in particular to a liquid chromatograph with an automatic cleaning function. Background Art

[0002] Liquid chromatography is a commonly used physical and chemical analysis instrument. It separates and quantitatively analyzes mixtures based on the differences in distribution coefficients, adsorption capacities, or other interaction forces between the stationary phase and the mobile phase of each component in the sample. It is widely used in biochemistry, drug analysis, food testing, environmental monitoring, and other fields, and has the characteristics of high resolution, high sensitivity, and high degree of automation.

[0003] Since some components in the sample may be adsorbed on the stationary phase of the chromatographic column or accumulated in other components of the system after each analysis, the liquid chromatograph needs to be cleaned after use. Usually, it is cleaned by using pure water and detergent in the form of an internal circulation mobile phase. However, since the chromatographic column is expensive and cannot be rinsed with pure water for a long time, the chromatographic column needs to be removed each time the liquid chromatograph is cleaned to prevent it from being damaged. This also means that the chromatographic column needs to be disassembled and assembled each time it is used, which is a cumbersome operation. Summary of the Invention

[0004] In order to overcome the shortcomings of the above-mentioned prior art, the present invention provides a liquid chromatograph with an automatic cleaning function, which does not require repeated disassembly and assembly of the chromatographic column, can simplify the operation of the experimenter and improve the fluency of the liquid chromatograph during analytical tests.

[0005] The technical solution of the present invention is: a liquid chromatograph with automatic cleaning function, comprising a body, a casing, a liquid reservoir, a chromatographic column and a protective column, wherein the casing is rotatably arranged on the body, the liquid reservoir is installed inside the body and can be covered by the casing, the chromatographic column is detachably installed on the right side of the body, the protective column is detachably installed on the right side of the body, and the bottom end of the chromatographic column and the top end of the protective column are connected by a pipeline, and further comprising a mounting block, a three-way valve sleeve, a three-way valve core, a three-way sleeve, an electromagnetic valve and a connecting Tube, the mounting block is arranged on the right side of the inside of the body, above the top of the chromatographic column, the three-way valve sleeve is arranged on the mounting block, the three-way valve sleeve is provided with three openings, upper, right and lower, the chromatographic column is connected with the lower opening of the three-way valve sleeve, the liquid reservoir is connected with the upper opening of the three-way valve sleeve, the three-way valve core is rotatably arranged in the three-way valve sleeve, the communicating pipe is arranged at the right opening of the three-way valve sleeve, and a three-way sleeve is arranged between the bottom end of the communicating pipe and the bottom end of the protective column, and the solenoid valve is arranged on the three-way sleeve.

[0006] Furthermore, it also includes a liquid dispensing mechanism, which includes a storage frame, a raw liquid frame, a mounting frame, a liquid storage tank and a liquid pipetting syringe. The storage frame is arranged on the top of the body, the raw liquid frame is fixedly arranged on the rear side of the storage frame, the liquid storage tanks are arranged in pairs in the storage frame, the mounting frame is fixedly arranged on the upper part of the storage frame, the liquid pipetting syringes are symmetrically installed on the mounting frame, and the output end of the liquid pipetting syringe is connected to the nearest liquid storage tank through a pipeline.

[0007] Furthermore, the raw liquid frame is provided with at least three compartments, the liquid storage tank can store pure water or cleaning agent, and both of the liquid storage tanks are connected to the liquid reservoir.

[0008] Furthermore, the liquid dispensing mechanism also includes an infusion tube, a one-way valve I and a one-way valve II. The infusion tube is connected to the input port of the aspiration syringe, and the infusion tube can extend into the compartment of the raw liquid frame. The one-way valve I is installed on the pipeline between the aspiration syringe and the liquid storage tank, and the one-way valve II is installed at the output end of the infusion tube.

[0009] Furthermore, a switching mechanism is also included, which includes a slide rail, a guide tube and a docking joint. The slide rail is installed on the top of the raw liquid frame. There are at least two guide tubes, and both are arranged on the slide rail in a through-type manner and can extend into the compartment corresponding to the raw liquid frame. The docking joint is installed at the input end of the infusion tube for conveying the cleaning agent, and the docking joint can slide on the slide rail and complete docking with the top of the guide tube.

[0010] Furthermore, the switching mechanism also includes a guide rod and a slide, the guide rod is fixedly arranged on the side of the slide rail close to the liquid storage tank, the slide is slidably arranged on the guide rod, and a return spring is arranged between the slide and the guide rod, and the slide is fixedly connected to the docking joint.

[0011] Furthermore, a blocking block is included, which is slidably arranged on the side of the raw liquid frame close to the liquid storage tank. A spring is arranged between the blocking block and the raw liquid frame, and the blocking block can also contact the slide and prevent the slide from resetting.

[0012] Furthermore, it also includes a driving mechanism, which includes an electric push rod and a linkage bar. The electric push rod is installed on the mounting frame, and a linkage bar is provided between the movable end of the electric push rod and the piston column of the pipette syringe positioned on the left. When the movable end of the electric push rod is extended, it can push the piston column of the pipette syringe down.

[0013] Furthermore, the driving mechanism also includes a driving rod and a triangular shift block, the triangular shift block is rotatably arranged on the side of the raw liquid frame close to the liquid storage tank, and a torsion spring is arranged between the triangular shift block and the raw liquid frame, the triangular shift block can contact the blocking block, the driving rod is fixedly arranged on the linkage bar, and when the driving rod descends or rises, it can contact the triangular shift block and force the triangular shift block to rotate clockwise or counterclockwise.

[0014] The beneficial effects are as follows: 1. The present invention, through the arrangement of the three-way valve sleeve, the three-way valve core and the three-way sleeve, controls the flow direction of pure water or cleaning agent by rotating the three-way valve core, so that the experimenter can freely control whether it flows through the chromatographic column and the guard column, thereby avoiding the chromatographic column being flushed with pure water for a long time, and the operation is convenient, without the need to repeatedly disassemble and assemble the chromatographic column and the guard column; 2. Through the setting of the liquid dispensing mechanism, the pure water or cleaning agent in the stock liquid frame is sucked by the liquid suction syringe and then injected into the liquid storage tank. The amount of various cleaning agents can be accurately controlled through the scale on the liquid suction syringe, so that the cleaning effect of the final configuration of the cleaning agent is better; 3. Through the setting of the switching mechanism and the driving mechanism, when configuring the cleaning liquid, the electric push rod can be used to push the piston column of the liquid aspiration syringe, which is more convenient to operate. After completing the absorption of the initial cleaning agent, the blocking block will be pushed down with the cooperation of the driving rod and the triangular push block, and the docking joint will then slide together with the slide plate to the next guide pipe to extract the next type of cleaning agent, further simplifying the operation of the test personnel. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a schematic diagram of the overall structure of the present invention.

[0016] Figure 2 It is a schematic diagram of the internal structure of the present invention.

[0017] Figure 3 It is a schematic structural diagram of the chromatographic column and the guard column of the present invention.

[0018] Figure 4 This is an enlarged view of part A of the present invention.

[0019] Figure 5 This is a structural exploded view of the three-way valve sleeve and the three-way valve core of the present invention.

[0020] Figure 6 This is an enlarged view of part B of the present invention.

[0021] Figure 7 It is a structural schematic diagram of the liquid dispensing mechanism of the present invention.

[0022] Figure 8 Schematic diagram of the structure of the switching mechanism of the present invention.

[0023] Figure 9 Schematic diagram of the structure of the driving mechanism of the present invention.

[0024] Figure 10 It is a partial structural diagram of the driving mechanism of the present invention.

[0025] In the accompanying drawings: 1-body, 11-casing, 12-liquid reservoir, 13-chromatographic column, 14-guard column, 2-mounting block, 21-three-way valve sleeve, 22-three-way valve core, 23-three-way sleeve, 24-solenoid valve, 25-connecting pipe, 3-liquid dispensing mechanism, 31-storage frame, 32-stock liquid frame, 33-mounting frame, 34-liquid storage tank, 35-pipetting syringe, 36-infusion tube, 37-one-way valve I, 38-one-way valve II, 4-switching mechanism, 41-slide rail, 42-flow guide tube, 43-docking joint, 44-guide rod, 45-slide plate, 5-driving mechanism, 51-electric push rod, 52-linkage bar, 53-driving rod, 54-blocking block, 55-triangular shifting block. DETAILED DESCRIPTION

[0026] Exemplary embodiments will be described in detail herein, examples of which are illustrated in the accompanying drawings. When the following description refers to the drawings, identical numbers in different figures represent identical or similar elements unless otherwise indicated. The embodiments described in the following exemplary embodiments are not intended to represent all embodiments consistent with the present application. Instead, they are merely examples of apparatus and methods consistent with certain aspects of the present application, as detailed in the appended claims.

[0027] See Figure 1-Figure 3 An embodiment of the present invention provides a liquid chromatograph with an automatic cleaning function, comprising a body 1, a casing 11, a liquid reservoir 12, a chromatographic column 13 and a protective column 14. The casing 11 is rotatably mounted on the body 1, and the casing 11 can provide protection for parts inside the body 1. A liquid reservoir 12 is provided inside the body 1, and a liquid to be analyzed or a cleaning agent is temporarily stored in the liquid reservoir 12. A chromatographic column 13 and a protective column 14 are detachably mounted on the right side of the body 1. The bottom outlet of the chromatographic column 13 and the top inlet of the protective column 14 are connected by a pipeline, and the bottom outlet of the protective column 14 is connected to the analyzer through a pipeline.

[0028] See Figure 3-Figure 6In the embodiment of the present invention, it also includes a mounting block 2, a three-way valve sleeve 21, a three-way valve core 22, a three-way sleeve 23, a solenoid valve 24 and a connecting pipe 25. The mounting block 2 is provided at the upper right position of the body 1 by bolts. The mounting block 2 is fixedly connected to the three-way valve sleeve 21. The three-way valve sleeve 21 is provided with three openings, which are respectively facing upward, right and downward. The chromatographic column 13 is connected to the lower opening of the three-way valve sleeve 21, and the liquid reservoir 12 is connected to the upper opening of the three-way valve sleeve 21 through a pipeline, and the three-way valve A three-way valve core 22 is rotatably provided in the sleeve 21. In the initial state, the three-way valve core 22 can form a passage between the liquid reservoir 12 and the chromatographic column 13. A connecting pipe 25 is connected to the right opening of the three-way valve sleeve 21. A three-way sleeve 23 is provided between the bottom end of the connecting pipe 25 and the bottom end of the protective column 14. A solenoid valve 24 is installed on the three-way sleeve 23. The three-way valve core 22 rotates 90° clockwise to close the initial passage and form a passage between the liquid reservoir 12 and the connecting pipe 25, thereby directly guiding the liquid to the analyzer.

[0029] In an embodiment of the present invention, an operator can inject the liquid to be analyzed into the liquid reservoir 12, so that it passes through the chromatographic column 13 and the guard column 14 at a uniform speed, and finally analyze the data thereof through an analytical instrument. After the analysis is completed, pure water can be first injected into the liquid reservoir 12, so that the pure water slowly flows through the chromatographic column 13 and the guard column 14 for a period of time, and then the three-way valve core 22 is rotated 90° clockwise to block the passage between the liquid reservoir 12 and the chromatographic column 13, and at the same time form a passage between the liquid reservoir 12 and the connecting pipe 25, so that the pure water is directly directed to the analyzer. If a cleaning agent is needed to clean the chromatographic column 13 later, the three-way valve core 22 can be turned back without repeatedly disassembling and assembling the chromatographic column 13 and the guard column 14.

[0030] See Figure 7In the embodiment of the present invention, a liquid dispensing mechanism 3 is also included. The liquid dispensing mechanism 3 is composed of a storage frame 31, a raw liquid frame 32, a mounting frame 33, a liquid storage tank 34, a liquid pipette 35, an infusion tube 36, a one-way valve I 37 and a one-way valve II 38. The storage frame 31 is installed on the top of the body 1. The raw liquid frame 32 is welded to the rear side of the storage frame 31. Three compartments are opened inside the raw liquid frame 32. Pure water is stored in the compartment on the right, and different types of cleaning agents are stored in the compartments on the left and in the middle. Two liquid storage tanks 34 are placed in the storage frame 31. The liquid storage tank 34 on the left is used For configuring cleaning agent, the liquid storage tank 34 on the right is used for quantitative storage of pure water. A mounting bracket 33 is fixed on the upper part of the storage frame 31 by bolts. Two pipetting syringes 35 are symmetrically installed on the mounting bracket 33. The pipetting syringe 35 is connected to the nearest liquid storage tank 34 through a pipeline, and the input port of the pipetting syringe 35 is connected to an infusion tube 36. The infusion tube 36 can extend into the corresponding compartment in the raw liquid frame 32, and a one-way valve II 38 is installed at the output end of the infusion tube 36. A one-way valve I 37 is installed on the pipeline between the pipetting syringe 35 and the liquid storage tank 34.

[0031] In an embodiment of the present invention, if the test personnel need to take the pure water or cleaning agent in the raw liquid frame 32, they must first close the one-way valve I37, then pull down the piston column of the liquid aspiration syringe 35, and control the amount according to the scale on the liquid aspiration syringe 35. After the extraction is completed, close the one-way valve II38, open the one-way valve I37, and then push up the piston column of the liquid aspiration syringe 35, so that the liquid absorbed in the liquid aspiration syringe 35 flows into the liquid storage tank 34, and finally enters the liquid reservoir 12.

[0032] See Figure 8 and Figure 9 In the embodiment of the present invention, a blocking block 54 and a switching mechanism 4 are also included. The switching mechanism 4 is composed of a slide rail 41, a guide tube 42, a docking joint 43, a guide rod 44 and a slide plate 45. The top of the raw liquid frame 32 is welded with a slide rail 41. Two guide tubes 42 are provided through the slide rail 41. The two guide tubes 42 extend into the two compartments for storing cleaning agents in the raw liquid frame 32 respectively. A docking joint 43 is installed on the output end of the infusion tube 36 on the left. The docking joint 43 can slide on the slide rail 41 and dock with the top of the guide tube 42. A guide rod 44 is fixedly connected to the front side of the slide rail 41, and a slide plate 45 is slidably provided on the guide rod 44, and a return spring is provided between the slide plate 45 and the guide rod 44. The slide plate 45 is also fixedly connected to the docking joint 43. Under the action of the return spring, the slide plate 45 can drive the docking joint 43 to slide synchronously. A blocking block 54 is slidably provided on the left position of the front side of the raw liquid frame 32, and a spring is provided between the blocking block 54 and the raw liquid frame 32. Under the action of the spring, the blocking block 54 can initially block the slide plate 45 to prevent it from sliding to the right.

[0033] See Figure 9 and Figure 10 In the embodiment of the present invention, a driving mechanism 5 is also included, which consists of an electric push rod 51, a linkage bar 52, a driving rod 53 and a triangular shifting block 55. The electric push rod 51 is installed on the mounting frame 33, and the movable end of the electric push rod 51 faces downward, and a linkage bar 52 is provided between its movable end and the piston column of the liquid-absorbing syringe 35 located on the left. When the movable end of the electric push rod 51 is extended, the piston column of the liquid-absorbing syringe 35 can be pushed down to extract the cleaning agent in the stock liquid frame 32. A triangular shifting block 55 is rotatably provided on the left side of the front side of the stock liquid frame 32, and a torsion spring is provided between the triangular shifting block 55 and the stock liquid frame 32. A driving rod 53 is fixedly provided on the linkage bar 52. When the driving rod 53 descends or rises, it can contact the triangular shifting block 55 and force the triangular shifting block 55 to rotate clockwise or counterclockwise.

[0034] In the embodiment of the present invention, if the test personnel need to configure the cleaning agent, they can start the electric push rod 51 to lower its movable end, drive the piston column of the liquid-absorbing syringe 35 to descend to a predetermined height, and extract the cleaning agent in the left compartment of the stock liquid frame 32. At this time, the driving rod 53 will then descend to contact the upper edge of the triangular shift block 55, and disengage from the upper edge after pressing the triangular shift block 55 to rotate clockwise at a certain angle. In this process, the right edge of the triangular shift block 55 will change from being in close contact with the lower surface of the blocking block 54 to being in close contact with the blocking block 5 under the action of the torsion spring. 4, and then the movable end of the electric push rod 51 is reset, the driving rod 53 rises accordingly, and contacts the upper edge of the triangular shift block 55 again, while pulling the triangular shift block 55 to rotate counterclockwise, and the right edge of the triangular shift block 55 immediately presses the blocking block 54 to slide down, and the blocking block 54 no longer hinders the slide plate 45. The slide plate 45 is then pulled to the right by the return spring, and the docking head 43 slides to the right on the slide rail 41 and completes docking with the guide pipe 42 on the right. Subsequently, the second cleaning agent can be extracted and finally configured in the liquid storage tank 34 on the left.

[0035] The above description is merely a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any modifications or substitutions that can be easily conceived by a person skilled in the art within the technical scope disclosed in the present invention should be included in the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be based on the scope of protection of the claims.

Claims

1. A liquid chromatograph with an automatic cleaning function, comprising a body (1), a casing (11), a liquid reservoir (12), a chromatographic column (13) and a protective column (14), wherein the casing (11) is rotatably mounted on the body (1), the liquid reservoir (12) is mounted inside the body (1) and can be covered by the casing (11), the chromatographic column (13) is detachably mounted on the right side of the body (1), the protective column (14) is detachably mounted on the right side of the body (1), and the bottom end of the chromatographic column (13) and the top end of the protective column (14) are connected through a pipeline; Its characteristics are: It also includes a mounting block (2), a three-way valve sleeve (21), a three-way valve core (22), a three-way sleeve (23), a solenoid valve (24) and a connecting pipe (25), wherein the mounting block (2) is arranged on the right side of the interior of the body (1) and is located above the top of the chromatographic column (13), the three-way valve sleeve (21) is arranged on the mounting block (2), and the three-way valve sleeve (21) is provided with three openings at the upper right and lower sides, and the chromatographic column (13) and the three-way valve sleeve are connected to each other. The lower opening of the sleeve (21) is connected, the liquid reservoir (12) is connected to the upper opening of the three-way valve sleeve (21), the three-way valve core (22) is rotatably arranged in the three-way valve sleeve (21), the connecting pipe (25) is arranged at the right opening of the three-way valve sleeve (21), and a three-way sleeve (23) is arranged between the bottom end of the connecting pipe (25) and the bottom end of the protection column (14), and the solenoid valve (24) is arranged on the three-way sleeve (23).

2. The liquid chromatograph with automatic cleaning function according to claim 1, wherein: The invention also includes a liquid dispensing mechanism (3), the liquid dispensing mechanism (3) including a storage frame (31), a raw liquid frame (32), a mounting frame (33), a liquid storage tank (34) and a liquid suction syringe (35), the storage frame (31) is arranged on the top of the body (1), the raw liquid frame (32) is fixedly arranged on the rear side of the storage frame (31), the liquid storage tanks (34) are arranged in pairs in the storage frame (31), the mounting frame (33) is fixedly arranged on the upper part of the storage frame (31), the liquid suction syringes (35) are symmetrically mounted on the mounting frame (33), and the output end of the liquid suction syringe (35) is connected to the nearest liquid storage tank (34) through a pipeline.

3. The liquid chromatograph with automatic cleaning function according to claim 2, characterized in that: The raw liquid frame (32) is provided with at least three compartments, the liquid storage tank (34) can store pure water or cleaning agent, and both liquid storage tanks (34) are connected to the liquid reservoir (12).

4. The liquid chromatograph with automatic cleaning function according to claim 3, characterized in that: The liquid dispensing mechanism (3) further includes an infusion tube (36), a one-way valve I (37) and a one-way valve II (38). The infusion tube (36) is connected to the input port of the liquid aspiration syringe (35), and the infusion tube (36) can extend into the compartment of the raw liquid frame (32). The one-way valve I (37) is installed on the pipeline between the liquid aspiration syringe (35) and the liquid storage tank (34), and the one-way valve II (38) is installed at the output end of the infusion tube (36).

5. The liquid chromatograph with automatic cleaning function according to claim 4, characterized in that: The invention also includes a switching mechanism (4), the switching mechanism (4) including a slide rail (41), a guide tube (42) and a docking joint (43), the slide rail (41) being mounted on the top of the raw liquid frame (32), at least two guide tubes (42) being provided, and both being arranged on the slide rail (41) in a through-type manner and being able to extend into the compartment corresponding to the raw liquid frame (32), the docking joint (43) being mounted on the input end of the infusion tube (36) for conveying the cleaning agent, and the docking joint (43) being able to slide on the slide rail (41) and dock with the top end of the guide tube (42).

6. The liquid chromatograph with automatic cleaning function according to claim 5, characterized in that: The switching mechanism (4) further comprises a guide rod (44) and a slide plate (45), wherein the guide rod (44) is fixedly arranged on a side of the slide rail (41) close to the liquid storage tank (34), the slide plate (45) is slidably arranged on the guide rod (44), and a return spring is arranged between the slide plate (45) and the guide rod (44), and the slide plate (45) is fixedly connected to the docking joint (43).

7. The liquid chromatograph with automatic cleaning function according to claim 6, characterized in that: The invention also includes a blocking block (54), which is slidably arranged on a side of the raw liquid frame (32) close to the liquid storage tank (34), and a spring is arranged between the blocking block (54) and the raw liquid frame (32). The blocking block (54) can also contact the slide plate (45) and prevent the slide plate (45) from resetting.

8. The liquid chromatograph with automatic cleaning function according to claim 7, characterized in that: The utility model further comprises a driving mechanism (5), wherein the driving mechanism (5) comprises an electric push rod (51) and a linkage bar (52), wherein the electric push rod (51) is mounted on the mounting frame (33), and a linkage bar (52) is provided between the movable end of the electric push rod (51) and the piston column of the liquid-absorbing syringe (35) located on the left, and when the movable end of the electric push rod (51) is extended, the piston column of the liquid-absorbing syringe (35) can be pushed down.

9. The liquid chromatograph with automatic cleaning function according to claim 8, characterized in that: The driving mechanism (5) further comprises a driving rod (53) and a triangular shifting block (55), wherein the triangular shifting block (55) is rotatably arranged on a side of the raw liquid frame (32) close to the liquid storage tank (34), and a torsion spring is arranged between the triangular shifting block (55) and the raw liquid frame (32), and the triangular shifting block (55) can contact the blocking block (54), and the driving rod (53) is fixedly arranged on the linkage bar (52), and when the driving rod (53) descends or ascends, it can contact the triangular shifting block (55) and force the triangular shifting block (55) to rotate clockwise or counterclockwise.

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