Cell separation sampling instrument

Abstract

The invention belongs to the technical field of cell separation, and relates to a cell separation sampling instrument which is mainly composed of a shell, a centrifugal assembly and a sampling assembly, the centrifugal assembly is arranged in the shell and comprises a first telescopic rod, a second supporting rod, a third supporting rod and the like, and the third supporting rod is pushed through a hydraulic rod of the first telescopic rod; the second supporting rod and the rotating disc are linked, the centrifugal bin rotates around the square section of the rotating shaft, then the angle of the centrifugal bin is adjusted, the sampling assembly is arranged on the left side and the upper portion of the shell and can sample and collect separated blood, and during centrifugation, a test tube can be fastened, collision damage is avoided, rapid acceleration and deceleration are achieved, and the centrifugation time is shortened; meanwhile, the sampling assembly does not need to frequently replace the needle head, so that the sampling operation time is shortened, the working efficiency is integrally improved, the equipment cost is reduced, and a more efficient and practical tool is provided for cell separation research.

Description

Technical Field

[0001] The invention belongs to the technical field of cell separation, in particular to a cell separation sampling instrument. Background Art

[0002] In the field of cell separation technology, cell separation sampling is a key step in cell biology and cytopathology research. Currently, the commonly used density gradient centrifugation method faces numerous challenges in practical application. For one thing, traditional centrifuges lack compatibility with test tube sizes, making operation cumbersome and easily affecting sample quality. Furthermore, existing equipment often utilizes complex and expensive variable-speed motor systems to achieve varying centrifugal speeds and gradients, increasing equipment cost, maintenance complexity, and energy consumption.

[0003] In view of this, in order to overcome the above technical problems, the present invention designs and develops a cell separation sampler to solve the above technical problems. Summary of the Invention

[0004] The present invention aims to overcome the problems of high equipment cost and limited speed and slope adjustment in the prior art, and to provide a cell separation sampler that utilizes a low-cost single-speed motor to meet different speed and slope requirements by precisely adjusting the centrifugal bin angle and the distance between the centrifugal bin and the center of the rotating shaft, thereby reducing equipment costs.

[0005] In order to achieve the above object, the present invention provides the following technical solutions:

[0006] The present invention provides a cell separation sampling instrument, comprising: a housing

[0007] A centrifuge assembly is provided in a housing, and the centrifuge assembly separates blood; the centrifuge assembly comprises a No. 1 telescopic rod, a No. 2 support rod, and a No. 3 support rod; the hydraulic rod of the No. 1 telescopic rod is fixedly connected to the No. 3 support rod, the No. 3 support rod is connected to the outer ring of the No. 2 bearing, and a rotating disk fixedly connected by bolts above the inner ring of the No. 2 bearing; the rotating disk and the No. 2 support rod are rotatably connected by a No. 1 rotating shaft; the end of the No. 2 support rod is rotatably connected to the No. 1 rotating shaft at the bottom of the centrifugal bin; the centrifugal bin is rotatably connected to the square section of the rotating shaft by the No. 2 rotating shaft;

[0008] The sampling component is arranged on the left side and the top of the shell, and the sampling component samples and collects the separated blood.

[0009] Preferably, the centrifugal assembly includes a first motor fixedly connected to the housing;

[0010] Bevel gear No. 1, fixedly connected to the output shaft of motor No. 1;

[0011] Bevel gear number two, meshing vertically with bevel gear number one;

[0012] The upper end of the No. 1 support rod is fixedly connected to the lower end of the No. 2 bevel gear, and the lower end of the No. 1 support rod is rotatably connected to the bottom of the housing through the No. 1 bearing;

[0013] The rotating shaft has a square section at the upper end and a cylindrical section at the lower end, and the lower end of the rotating shaft is fixedly connected to the upper end of the second bevel gear;

[0014] A chute, wherein four of the chute are provided on the cylindrical section of the rotating shaft;

[0015] The second bearing is sleeved on the cylindrical section of the rotating shaft. The upper portion of the inner ring of the second bearing is fixedly connected to the rotating disk by bolts. The upper portion of the rotating disk is rotatably connected to the first rotating shaft.

[0016] Slide blocks, wherein four of the slide blocks are respectively arranged on the side walls of the inner ring of the second bearing, and the four slide blocks slide in four slide grooves respectively;

[0017] A second rotating shaft, wherein four of the second rotating shafts are rotatably connected to the four side walls of the square end of the rotating shaft through rotating brackets;

[0018] Centrifugal bins, wherein the four centrifugal bins are rotatably connected to the rotating shaft via a No. 2 rotating shaft, and the bottoms of the four centrifugal bins are rotatably connected to a No. 3 rotating shaft;

[0019] The two ends of the four No. 2 support rods are respectively connected to the No. 3 rotating shaft at the lower end of the centrifugal bin and the No. 1 rotating shaft at the upper end of the rotating disk;

[0020] Threaded rods, wherein the four threaded rods are respectively threadedly connected to the side walls of the centrifugal bin and extend into the centrifugal bin, and one end of the threaded rod extending into the centrifugal bin is an optical axis;

[0021] The push plate is rotatably connected to one end of the optical shaft of the threaded rod extending into the centrifugal bin through a No. 3 bearing;

[0022] a knob fixedly connected to the other end of the threaded rod;

[0023] The sponge plate is arranged on the side wall of the centrifugal bin and the other side of the push plate;

[0024] Telescopic rod No. 1, arranged at the bottom end of the shell;

[0025] The third support rod is arranged parallel to the housing, and one end of the third support rod is fixedly connected to the bottom of the outer ring of the second bearing. The third support rod is fixedly connected to the extension rod of the first telescopic rod near the middle position;

[0026] The No. 1 fixing block is fixedly connected to the other end of the No. 3 support rod and is used to balance the force of the No. 3 support rod;

[0027] Preferably, the sampling assembly includes an aspirator provided on the side wall of the housing;

[0028] No. 2 hydraulic press, installed on the side wall of the aspirator;

[0029] The control compartment is threadedly connected to the front end of the hydraulic rod of the No. 2 hydraulic machine, and three circular holes are opened at the lower end of the control compartment;

[0030] No. 2 motor, three of the No. 2 motors are arranged in the control compartment;

[0031] Lead screws, the three lead screws are fixedly connected to the output shafts of the three second motors respectively, and the other ends extend out of the control compartment through the circular holes;

[0032] The transport needle is fixedly connected to the lower end of the hydraulic rod of the No. 2 hydraulic machine;

[0033] Bending needles, three of which are arranged in the transport needle, and the three bending needles are respectively connected to the lead screw through threaded blocks arranged thereon;

[0034] Transport hoses, one end of each of the three transport hoses is fixedly connected to the three bent needles, and the other end passes through the hole on the control compartment and is connected to the aspirator;

[0035] The photoelectric liquid level sensor is arranged at the bottom end of the inner side of the transport needle and is connected to the three No. 2 motors in the control compartment through a data line arranged in the side wall of the transport needle.

[0036] Preferably, a plurality of loose elastic bands are fixedly connected in the centrifugal bin, and the width of the loose elastic bands is the same as the height of the centrifugal bin.

[0037] Preferably, the lower ends of the three lead screws are respectively fixedly connected to a No. 2 fixing block.

[0038] Preferably, the transport hose is an elastic and retractable transport hose.

[0039] Preferably, the push plate is made of hard plastic.

[0040] Preferably, the plurality of loose elastic bands are staggered and connected in the centrifugal bin and are vertically fixedly connected in the centrifugal bin.

[0041] Preferably, a baffle is fixedly connected inside the transport needle; one end of the bent needle passes through the baffle and is sealed and slidably connected to the baffle.

[0042] Preferably, the connection between the square section of the rotating shaft and the second rotating shaft can be set as a telescopic rod.

[0043] The beneficial effects of the present invention are as follows:

[0044] 1. The cell separation sampler provided by the present invention is provided with a centrifugal assembly to tighten the test tube during centrifugation to prevent the test tube from being damaged by collision during the centrifugation process. At the same time, because the test tube is tightened, the centrifugal assembly can quickly speed up and slow down, reach the maximum speed more quickly, shorten the centrifugation time, and improve work efficiency. At the same time, a sampling assembly is designed on the original extraction system, and there is no need to replace a needle every time a layer of liquid is extracted, which shortens the extraction operation time, further shortens the entire working time, and improves work efficiency.

[0045] 2. The cell separation sampler provided by the present invention divides the centrifugal bin into multiple small bins by arranging multiple loose elastic bands in the centrifugal bin, which can fasten and separate test tubes of different sizes. Multiple test tubes can also be placed in one centrifugal bin, and the multiple test tubes are separated by loose elastic bands, thereby avoiding collision and wear between the multiple test tubes during the separation process, realizing the separation operation of multiple test tubes and test tubes of different sizes, and further improving the separation efficiency.

[0046] 3. The cell separation sampler of the present invention, with its unique centrifugal component design, uses a low-cost single-speed No. 1 motor, which reduces the manufacturing cost of the equipment and reduces the maintenance cost caused by complex variable-speed motors. By flexibly adjusting the angle of the centrifugal bin and its distance from the center of the rotating shaft, it can meet the requirements of various cell separation experiments for different centrifugal speeds and slopes, significantly improving the effect and quality of cell separation. In addition, combined with the optimized sampling component, it can accurately sample under different centrifugal conditions, further improving the overall work efficiency, and providing a more cost-effective and practical equipment option for the widespread application of cell separation technology in scientific research, clinical and other fields. BRIEF DESCRIPTION OF THE DRAWINGS

[0047] The present invention will be further described below with reference to the accompanying drawings.

[0048] Figure 1 It is the main figure of the present invention;

[0049] Figure 2 is a top sectional view of the centrifugal bin of the present invention;

[0050] Figure 3 It is a schematic diagram of the structure of the first rotating shaft of the present invention;

[0051] Figure 4 is a partial cross-sectional view of the sampling assembly of the present invention;

[0052] Figure 5 This is a schematic diagram of the top view of the No. 2 bearing of the present invention;

[0053] Figure 6 It is a schematic structural diagram of the centrifugal bin portion of the present invention;

[0054] Figure 7 It is a schematic diagram of the top view of the rotating disk of the present invention.

[0055] In the figure: 1, housing, 2, motor No. 1, 3, bevel gear No. 1, 4, bearing No. 1, 5, support rod No. 1, 6, bevel gear No. 2, 7, telescopic rod No. 1, 8, fixing block No. 1, 9, support rod No. 2, 10, bearing No. 2, 11, shaft No. 1, 12, support rod No. 3, 13, centrifugal position, 14, knob, 15, shaft No. 2, 16, threaded rod, 17, slide, 18, transport needle, 19, control chamber, 2 0. Transport hose, 21. Hydraulic press No. 2, 22. Push plate, 23. Sponge plate, 24. Loose elastic band, 25. Data cable, 26. Motor No. 2, 27. Threaded block, 28. Lead screw, 29. Fixed block No. 2, 30. Baffle, 31. Bent needle, 32. Photoelectric liquid level sensor, 33. Slider, 34. Round hole, 35. Hole, 36. Rotating shaft, 37. Aspirator, 38. Bearing No. 3, 39. Rotating disk. DETAILED DESCRIPTION

[0056] The following examples are combined with the attached Figure 1-7 The present invention is further described, but is not limited thereto.

[0057] Example 1:

[0058] The present invention provides a cell separation sampling instrument, comprising a housing 1,

[0059] A centrifugal assembly is provided in the housing 1 and separates blood; the centrifugal assembly comprises a No. 1 telescopic rod 7, a No. 2 support rod 9 and a No. 3 support rod 12, the hydraulic rod of the No. 1 telescopic rod 7 is fixedly connected to the No. 3 support rod 12, the No. 3 support rod 12 is connected to the outer ring of the No. 2 bearing 10, and a rotating disk 39 is fixedly connected with bolts above the inner ring of the No. 2 bearing 10, the rotating disk 39 and the No. 2 support rod 9 are rotatably connected by the No. 1 rotating shaft 11, the end of the No. 2 support rod 9 is rotatably connected to the No. 1 rotating shaft 11 at the bottom of the centrifugal bin 13, and the centrifugal bin 13 is rotatably connected to the square section of the rotating shaft 36 by the No. 2 rotating shaft 15;

[0060] The inclination angle of the centrifugal bin 13 in the centrifugal assembly of the present invention can be adjusted by the No. 1 telescopic rod 7 at the bottom. When the device starts the centrifugal program, the control unit drives the hydraulic rod of the No. 1 telescopic rod 17 to extend, pushing the No. 3 support rod 15 hinged thereto to move axially. Figure 3 Since the third support rod 15 is hinged to the second support rod 14 through the cylindrical section of the rotating shaft 36, the second support rod 14 will deflect around the axis of the rotating shaft 36. Figure 4As shown, the upward and downward adjustment of the first telescopic rod 7 in this application can precisely adjust the angle of the centrifugal bin 13 through a series of controls. By precisely controlling the extension and retraction length of the first telescopic rod 7 and coordinating the linkage mechanism consisting of the third support rod 12, the second bearing 10, the rotating disk 39, and the second support rod 9, the angle adjustment of the centrifugal bin is more precise.

[0061] The sampling component is arranged on the upper left side of the shell 1, and the sampling component samples and collects the separated blood.

[0062] The test tubes used in existing density gradient centrifuges vary in size. Most general centrifuges are usually suitable for only one size of test tube. If the blood to be tested is collected in a test tube that is different in size from the centrifuge, the blood needs to be transferred to a test tube of the appropriate size, which requires additional operations and prolongs the time for separation and sampling testing. When performing a centrifugal operation, the test tube containing blood is generally placed directly into a centrifugal container, and the centrifugal container is connected to the centrifuge by soft components such as rubber. When the centrifuge starts working, as the speed increases, the centrifugal container is thrown into a parallel state, that is, the test tubes in the centrifugal container are in a parallel state. When the centrifuge starts working, as the speed increases, the centrifugal container is thrown into a parallel state, that is, the test tubes in the centrifugal container are in a parallel state. After the centrifugation is completed, the centrifuge slowly returns to a vertical or nearly vertical state. During this process, the entire test tube is not fastened by the centrifuge container, so the test tube and the centrifuge container may collide during the start and end of centrifugation. If the operator does not put all the test tubes into the centrifuge container, the test tube may be thrown out of the centrifuge container. At the same time, because the test tube is not fastened, the centrifuge cannot be too fast in the two processes of starting to speed up and starting to slow down to prevent the test tube from being thrown out, and the entire operation process is also prolonged. When sampling after the separation is completed, a needle needs to be replaced each time a layer of liquid is extracted, which is more troublesome and further prolongs the operation time, affecting subsequent detection;

[0063] By setting up a centrifugal assembly, the test tube is tightened during centrifugation to prevent the test tube from colliding and being damaged during the centrifugation process. At the same time, because the test tube is tightened, the centrifugal assembly can quickly speed up and slow down, reach the maximum speed more quickly, shorten the centrifugation time, and improve work efficiency. The hydraulic device can adjust the centrifugal position angle to meet different needs, and the single-speed No. 1 motor meets different speed requirements. At the same time, a sampling assembly is designed on the original extraction system, and there is no need to replace a needle every time a layer of liquid is extracted, which shortens the extraction operation time, once again shortening the entire working time and improving work efficiency.

[0064] As a specific embodiment of the present invention, the centrifugal assembly includes a first motor 2 fixedly connected to the housing 1;

[0065] Bevel gear No. 1 3, fixedly connected to the output shaft of motor No. 1 2;

[0066] The second bevel gear 6 is vertically meshed with the first bevel gear 3;

[0067] The upper end of the No. 1 support rod 5 is fixedly connected to the lower end of the No. 2 bevel gear 6, and the lower end of the No. 1 support rod 5 is rotatably connected to the bottom of the housing 1 through the No. 1 bearing 4;

[0068] The rotating shaft 36 has a square section at the upper end and a cylindrical section at the lower end, and the lower end of the rotating shaft 36 is fixedly connected to the upper end of the second bevel gear 6;

[0069] Slide grooves 17, four of which are provided on the cylindrical section of the rotating shaft 36;

[0070] The second bearing 10 is sleeved on the cylindrical section of the rotating shaft 36. The upper part of the inner ring of the second bearing 10 is fixedly connected to the rotating disk 39 by bolts. The upper part of the rotating disk 39 is rotatably connected to the first rotating shaft 11 through a rotating bracket.

[0071] Slide blocks 33, four of which are respectively disposed on the inner ring sidewalls of the second bearing 10, and the four slide blocks 33 slide in the four slide grooves 17;

[0072] The second rotating shaft 15, the four second rotating shafts 15 are rotatably connected to the four end side walls of the square end of the rotating shaft 36 through rotating brackets;

[0073] The centrifugal bins 13 are rotatably connected to the rotating shaft 36 via the second rotating shaft 15, and the bottoms of the four centrifugal bins 13 are rotatably connected to a third rotating shaft;

[0074] The two ends of the four No. 2 support rods 9 are respectively connected to the No. 3 rotating shaft at the lower end of the centrifugal bin 13 and the No. 1 rotating shaft 11 at the upper end of the rotating disk 39;

[0075] Threaded rods 16, wherein the four threaded rods 16 are respectively threadedly connected to the side walls of the centrifugal bin 13 and extend into the centrifugal bin 13, and one end of the threaded rod 16 extending into the centrifugal bin 13 is an optical axis;

[0076] The push plate 22 is rotatably connected to one end of the optical axis of the threaded rod 16 extending into the centrifugal bin 13 through a No. 3 bearing 38;

[0077] The knob 14 is fixedly connected to the other end of the threaded rod 16;

[0078] The sponge plate 23 is provided on the side wall of the centrifugal bin 13 and the other side of the push plate 22;

[0079] A first telescopic rod 7 is provided at the bottom end of the housing 1;

[0080] The third support rod 12 is arranged parallel to the housing 1, and one end is fixedly connected to the bottom of the outer ring of the second bearing 10. The third support rod 12 is fixedly connected to the extension rod of the first telescopic rod 7 near the middle position;

[0081] The No. 1 fixing block 8 is fixedly connected to the other end of the No. 3 support rod 12 and is used to balance the force applied to the No. 3 support rod 12 .

[0082] The up and down adjustment of the No. 1 telescopic rod 7 in the present application can accurately realize the angle change of the centrifugal position through a series of controls. Specifically, when the hydraulic rod of the No. 1 hydraulic press 7 pushes the No. 3 support rod 12 to rise or fall, the No. 3 support rod 12 drives the No. 2 bearing 10 to move up and down along the cylindrical section of the rotating shaft 36. Since the slider 33 of the inner ring of the No. 2 bearing 10 cooperates with the slide groove 17 of the cylindrical section of the rotating shaft 36, the No. 2 bearing 10 slides up and down stably; as the No. 2 bearing 10 moves, the rotating disk 39 connected to it drives the No. 2 support rod 9 to move, thereby realizing the rotation of the centrifugal position 13 around the No. 2 rotating shaft 15.

[0083] In the present invention, a No. 1 motor 2 is fixedly connected to the inner bottom end of the housing 1, a No. 1 bevel gear 3 is fixedly connected to the output shaft of the No. 1 motor 2, a No. 2 bevel gear 6 is provided to be vertically meshed with the No. 1 bevel gear 3, a No. 1 support rod 5 is fixedly connected to the lower end of the No. 2 bevel gear 6, the other end of the No. 1 support rod 5 is rotatably connected to the housing 1 through a No. 1 bearing 4 provided at the bottom of the housing 1, and a rotating shaft 36 is fixedly connected to the upper end of the No. 2 bevel gear 6. The upper end of the rotating shaft 36 is set as a square section, and the lower end is set as a cylindrical section. Four symmetrically distributed sliding grooves 17 are processed on the surface of the cylindrical section of the rotating shaft 36 along the axial direction;

[0084] The No. 2 bearing 10 is mounted on the outer cylindrical surface of the cylindrical section of the rotating shaft 36. Four sliders 33 are fixedly connected to the sidewall of the inner ring of the No. 2 bearing 10. The positions of the four sliders 33 correspond to the positions of the four slide grooves 17. This allows the No. 2 bearing 10 to slide up and down within the cylindrical section of the rotating shaft 36 via the sliders 33 and slide grooves 17 while the inner ring of the No. 2 bearing 10 rotates synchronously with the rotating shaft 36. A rotating disk 39 is bolted above the No. 2 bearing 10. The rotating disk 39 is rotatably connected to the No. 1 rotating shaft 11.

[0085] Four No. 2 rotating shafts 15 are provided on the side walls of the square section at the upper end of the rotating shaft 36. Four square centrifugal bins 13 are rotatably connected to the square end side walls of the rotating shaft 36 via the No. 2 rotating shafts 15. A No. 1 rotating shaft 11 is provided at the lower end of the centrifugal bin 13 and the upper end of the rotating disk 39. Four No. 2 support rods 9 are provided with their ends rotatably connected to the No. 1 rotating shaft 11 on the centrifugal bin 13 and the upper end of the rotating disk 39 respectively.

[0086] When the No. 2 bearing 10 rises, the centrifugal bin 13 can be pushed to be parallel to the bottom surface through the No. 2 support rod 9. A threaded rod 16 is provided on the side wall of the centrifugal bin 13 and is threadedly connected to the centrifugal bin 13, and one end of the threaded rod 16 can extend into the centrifugal bin 13. The end of the threaded rod 16 extending into the centrifugal bin 13 is the optical axis. A push plate 22 is rotatably connected to the end of the optical axis of the threaded rod 16 extending into the centrifugal bin 13 through the No. 3 bearing 38. The optical axis can drive the push plate 22 to move. The end of the threaded rod 16 extending into the centrifugal bin 13 is located at the center of the push plate 22. The shape of the push plate 22 is the same as the shape of the side wall of the centrifugal bin 13, and the size of the push plate 22 is the same as the internal size of the centrifugal bin 13;

[0087] A knob 14 is fixedly connected to one end of the threaded rod 16 located outside the centrifugal bin 13, and a sponge plate 23 is installed on the other side of the side wall of the centrifugal bin 13 and the push plate 22, that is, the sponge plate 23 evenly covers the other three side walls of the centrifugal bin 13 and the left side wall of the push plate 22 except for the push plate 22. A No. 1 telescopic rod 7 is provided at the other end of the bottom end of the interior of the shell 1, and a No. 3 support rod 12 parallel to the ground is provided at the upper end of the No. 1 telescopic rod 7. The No. 3 support rod 12 is fixedly connected to the hydraulic rod of the No. 1 telescopic rod 7 near the middle position. One end of the No. 3 support rod 12 is fixedly connected to the lower end of the outer ring of the No. 2 bearing 10, and the other end is fixedly connected to a No. 1 fixed block 8;

[0088] The test tube to be separated is placed in the centrifugal bin 13, and then the knob 14 is rotated. When the threaded rod 16 rotates into the centrifugal bin 13, it pushes the push plate 22 close to the test tube through the No. 3 bearing 38, and the test tube is squeezed by the inner wall of the centrifugal bin 13 and the sponge plate 23 on the push plate 22 to tighten the test tube. After the sponge plate 23 on the push plate 22 and the sponge plate 23 in the centrifugal bin 13 have tightened the test tube, centrifugation is started. Because the sponge plate 23 is relatively soft and has a large friction force, the test tube will not be damaged when being tightened. At the same time, the test tube can be well stabilized to ensure that it will not be thrown out during the centrifugation process and will not collide with the centrifugal bin 13 and be damaged.

[0089] When the No. 1 motor 2 is started, the No. 1 telescopic rod 7 is started, and the No. 1 motor 2 drives the rotating shaft 36 to rotate through the No. 1 bevel gear 3 and the No. 2 bevel gear 6. During the acceleration process, the hydraulic rod of the No. 1 telescopic rod 7 pushes the No. 3 support rod 12 to rise. Since the force of the hydraulic rod on the No. 3 support rod 12 acts at the middle position of the No. 3 support rod 12, and the mass of the No. 1 fixed block 8 fixedly connected to the right end of the No. 3 support rod 12 is equal to the mass supported by the left end of the No. 3 support rod 12, this innovative design ensures that when the hydraulic rod of the No. 1 telescopic rod 7 pushes the No. 2 bearing 10 to rise and fall, the force acting on the No. 2 bearing 10 is always vertically upward, so that the No. 2 bearing 10 can be lifted and lowered stably, avoiding tilting failures and ensuring stable operation of the equipment; by tightening the test tube, the time for accelerating and decelerating the rotating shaft 36 is shortened compared with traditional centrifuges; the test tubes of traditional centrifuges are not tightened, and the acceleration and deceleration process needs to be carried out slowly to prevent the test tubes from being thrown out or damaged by collision. The present invention overcomes this disadvantage and significantly shortens the entire centrifugation time; this not only improves the efficiency of cell separation sampling, but also reduces the risk of sample damage during centrifugation, ensures sample quality, provides a more reliable sample basis for subsequent cell research and detection, and enhances the practicality and advancement of the equipment in the field of cell separation.

[0090] The deflection angle of the No. 2 support rod 14 is directly related to the tilt angle of the centrifugal bin 10. Specifically, when the No. 2 support rod 14 deflects upward, the centrifugal bin bracket 16 connected to its end drives the centrifugal bin 10 to tilt upward around the axis of the No. 2 rotating shaft 13, and the tilt angle of the centrifugal bin 10 increases with the increase of the extended length of the hydraulic rod; conversely, when the hydraulic rod contracts, the centrifugal bin 10 gradually returns to a horizontal state. Through such a design, the tilt angle of the centrifugal bin 10 can be accurately controlled to meet the centrifugation requirements of different cell samples. When separating cells of different densities, the optimal tilt angle can be quickly adjusted according to the characteristics of the cells, thereby improving the efficiency and quality of cell separation, making the separated cells more pure, reducing interference from impurities, and providing better quality samples for subsequent cell research and testing.

[0091] As a specific embodiment of the present invention, the sampling assembly includes an aspirator 37 provided on the side wall of the housing 1;

[0092] The second hydraulic press 21 is provided on the side wall of the aspirator 37;

[0093] The control chamber 19 is threadedly connected to the front end of the hydraulic rod of the second hydraulic press 21, and three circular holes 34 are opened at the lower end of the control chamber 19;

[0094] No. 2 motor 26, three No. 2 motors 26 are arranged in the control compartment 19;

[0095] Screws 28, three of the screws 28 are fixedly connected to the output shafts of the three second motors 26, and the other ends extend out of the control compartment 19 through the circular hole 34;

[0096] The transport needle 18 is fixedly connected to the lower end of the hydraulic rod of the second hydraulic press 21;

[0097] Bending needles 31, three of the bending needles 31 are arranged in the transport needle 18, and the three bending needles 31 are respectively connected to the lead screw 28 through the threaded blocks 27 provided thereon;

[0098] Transport hoses 20, one end of each of the three transport hoses 20 is fixedly connected to the three bent needles 31, and the other end passes through the hole 35 on the control chamber 19 and is connected to the aspirator 37;

[0099] The photoelectric liquid level sensor 32 is disposed at the bottom inner side of the transport needle 18 and is connected to the three No. 2 motors 26 in the control compartment 19 via a data line 25 disposed in the side wall of the transport needle 18 .

[0100] In the present invention, a conventional aspirator 37 used in existing cell separation and sampling devices is provided on the side wall of the housing 1. A No. 2 hydraulic press 21 is fixed to the side wall of the aspirator 37. A control chamber 19 is threadedly connected to the front end of the hydraulic rod of the No. 2 hydraulic press 21. Three circular holes 34 are formed at the lower end of the control chamber 19. Three No. 2 micro motors 26 are fixedly connected side by side to the inner side wall of the control chamber 19. A lead screw 28 is fixedly connected to the output shaft of each of the three No. 2 motors 26. The other end of the lead screw 28 extends out of the control chamber 19 through the circular hole 34. The diameter of the circular hole 34 is larger than that of the lead screw 28, so that the lead screw 28 does not come into contact with the control chamber 19.

[0101] A transport needle 18 is fixedly connected to the front end of the control chamber 19, and three bent needles 31 are provided in the transport needle 18. The needle openings of the three bent needles 31 are flat and face upward. When the needle is inserted into the test tube for aspirating liquid, the needle opening of the bent needle 31 is located between the two liquids and faces upward. The liquid aspirated is the upper layer of liquid, and the suction force is upward. Therefore, when the suction force is increased, that is, the aspiration speed is accelerated, the lower layer of liquid will not be aspirated, thereby accelerating the sampling speed after separation. The three bent needles 31 are respectively connected to the lead screw 28 through the threaded blocks 27 fixed thereon. A transport hose 20 is fixedly connected to the upper end of the bent needle 31. The other end of the transport hose 20 passes through the control chamber 19 and is connected to the aspirator 37. A hole 35 for the transport hose 20 to pass through is provided at the place where the transport hose 20 passes through the control chamber 19.

[0102] After the cell separation operation is completed, the sampling operation begins. The position of the centrifugal bin 13 in the housing 1 is manually adjusted to be directly below the transport needle 18. Then, the No. 2 hydraulic press 21 is started. The No. 2 hydraulic press 21 pushes the transport needle 18 gradually close to the test tube in the centrifugal bin 13 and inserts it. The photoelectric liquid level sensor 32 contains a near-infrared light-emitting diode and a photosensor. The light emitted by the light-emitting diode is guided into the lens at the top of the photoelectric liquid level sensor 32.

[0103] When the liquid submerges the lens of the photoelectric liquid level sensor 32, light is refracted into the liquid, and a small amount of light is received by the receiver. The photoelectric liquid level sensor 32 senses the change in the amount of received light, actuates the electrical switch, and controls the external circuit. Because the blood in the test tube has been clearly stratified after the separation operation, liquids of different colors absorb and refract light differently. When the photoelectric liquid level sensor 32 detects a significant change in the amount of light, it determines the position, actuates the electrical switch, and controls the external circuit to stop the transport needle 18 from descending.

[0104] At the same time, one of the three miniature No. 2 motors 26 is activated in sequence, controlling the bent needle 31 to reach the photoelectric liquid level sensor 32 through the lead screw 28. Because the bent needle 31 falls to the interface between the two liquid levels, it also affects the refraction of light by the liquid, that is, the amount of light received by the photoelectric liquid level sensor 32 changes again, and the electrical switch is driven again to control the external circuit, turning off the No. 2 motor 26 that is controlling the rotation of the lead screw 28. The photoelectric liquid level sensor 32 has accurate liquid level detection, high repeatability, fast response speed, and precise liquid level control, so it can ensure that the needle hole of the bent needle 31 is accurately located at the interface between the two liquid levels.

[0105] When the upper layer of liquid is absorbed, there is no liquid on the top of the photoelectric liquid level sensor 32, and all the light emitted by the light-emitting diode is absorbed by the receiver. The photoelectric liquid level sensor 32 then drives the electrical switch to control the external circuit to control the transport needle to descend, and repeats the above process until the three layers of liquid are absorbed. Compared with the traditional method of replacing a needle and reinserting it into the test tube and aspirating each time a layer of liquid is absorbed, not only is the sampling speed faster and the sampling efficiency higher, but the upward needle port also avoids the extraction of the lower layer of liquid and affecting the detection, thereby improving the purity of the sampled liquid.

[0106] As a specific embodiment of the present invention, a plurality of loose elastic bands 24 are vertically fixedly connected in the centrifugal bin 13 , and the width of the loose elastic bands 24 is the same as the height of the centrifugal bin 13 .

[0107] In the present invention, multiple loose elastic bands 24 are vertically fixedly connected in the centrifugal bin 13. The width of the loose elastic bands 24 is the same as the height of the centrifugal bin 13. When placing a small test tube, the screw-in size of the threaded rod 16 can be reduced, shortening the tightening time. When placing a large test tube, the large test tube can be preliminarily tightened first, which facilitates the subsequent threaded rod 16 to push the push plate 22 to tighten the large test tube.

[0108] As a specific embodiment of the present invention, the lower ends of the three lead screws 28 are respectively fixedly connected to a No. 2 fixing block 29 .

[0109] In order to avoid malfunction caused by the threaded block 27 disengaging from the screw 28 during rotation, a No. 2 fixed block 29 is fixedly connected to the lower end of the screw 28, which is equivalent to setting the maximum distance for the bent needle 31 to descend. If it has not reached the junction of the two liquid levels after reaching the maximum distance, the position can be adjusted by restarting the No. 2 hydraulic press 21.

[0110] As a specific embodiment of the present invention, the transport hose 20 is an elastic and retractable transport hose 20 .

[0111] The present invention takes into account that the bent needle 31 is raised and lowered along with the hydraulic rod of the No. 2 hydraulic press 21. Although the transport hose 20 passes through the control compartment 19 and a hole 35 for the transport hose 20 to pass through is provided, a certain pulling force will inevitably be generated on the transport hose 20 during the lifting process. In order to avoid damage to the transport hose 20 while not affecting the extraction of cell fluid, the transport hose 20 is required to be an elastic and retractable transport hose 20. Because the medical blood transfusion hose is made of latex, it has uniform wall thickness, good elasticity, and rebound. The inner and outer surfaces are specially treated and have strong anti-aging ability. The tensile force ratio can reach 1:7, and the minimum inner and outer diameter specifications can reach 2*4mm. Therefore, the medical blood transfusion hose can be used as the material of the transport hose 20 in the present invention.

[0112] As a specific embodiment of the present invention, the push plate 22 is made of hard plastic.

[0113] In the present invention, the centrifugal bin 13 cannot be too heavy, otherwise it will increase the pressure on the second shaft 15 and affect the service life of the second bearing 10. Therefore, the material of the push plate 22 is selected to be hard plastic.

[0114] As a specific embodiment of the present invention, the plurality of loose elastic bands 24 are staggered and connected in the centrifugal bin 13 and are vertically fixedly connected in the centrifugal bin 13 .

[0115] In the present invention, multiple loose elastic bands 24 vertically fixedly connected in the centrifugal bin 13 are staggered and connected, dividing the centrifugal bin 13 into multiple small bins. Since the width of the loose elastic band 24 is the same as the height of the centrifugal bin 13, the depth of the multiple small bins is also the same as the depth of the centrifugal bin 13. When multiple small test tubes are separated at one time, the small test tubes can be placed in multiple small bins, and the loose elastic band 24 can protect the small test tubes from being squeezed and rubbed against each other and damaged. When a single large test tube is centrifuged, the loose elastic band 24 can be stretched and expanded several times without being damaged, and the elasticity of the loose elastic band 24 can expand to the entire centrifugal bin 13. Therefore, it is only necessary to place the large test tube in the small bin in the middle, and the expanded loose elastic band 24 can fix the large test tube well, and then rotate the knob 14 to push the push plate 22 for further fixation.

[0116] As a specific embodiment of the present invention, a baffle 30 is fixedly connected inside the transport needle 18 ; one end of the bending needle 31 passes through the baffle 30 and is sealed and slidably connected to the baffle 30 .

[0117] Long-term use of the workpiece will cause wear. In order to avoid waste generated by the contact and wear between the screw 28 and the threaded block 27 during long-term use, and dust contacting the screw 28, and falling into the test tube during sampling to contaminate the cell fluid, the present invention has a baffle 30 fixedly connected to the transport needle 18, and one end of the bent needle 31 passes through the baffle 30 and is sealed and slidably connected to the baffle 30. When extraction is performed, the bent needle 31 is raised and lowered under the drive of the screw 28, and is sealed and slidably connected to the baffle 30 to prevent waste and dust from entering the test tube and contaminating the blood.

[0118] As a specific embodiment of the present invention, the connection between the square section of the rotating shaft (36) and the second rotating shaft (15) can be set as a telescopic rod.

[0119] In the present invention, when the centrifugal radius needs to be adjusted to suit different centrifugal requirements, the telescopic rod at the connection between the No. 2 rotating shaft 15 and the square section of the rotating shaft 36 and the No. 2 support rod 9 can be used to achieve this. Before performing the cell separation operation, the required centrifugal radius is determined according to the experimental requirements. The No. 1 telescopic rod 7 is activated, and the hydraulic rod of the No. 1 telescopic rod 7 pushes the No. 3 support rod 12 up or down. The No. 3 support rod 12 drives the No. 2 bearing 10 to move up and down along the cylindrical section of the rotating shaft 36. Since the raised slider 33 on the inner ring of the No. 2 bearing 10 cooperates with the slide groove 17 of the cylindrical section of the rotating shaft 36, the No. 2 bearing 10 can slide up and down stably. As the No. 2 bearing 10 moves, the No. 2 support rod 9 will also move accordingly. At this time, the telescopic rod between the No. 2 rotating shaft 15 and the square section of the rotating shaft 36 plays a key role. The telescopic rod enables the centrifugal bin 13 to move horizontally. As bearing No. 2 (10) moves upward, support rod No. 2 (9) pushes the bottom of centrifugal chamber 13 outward. Simultaneously, rotating shaft No. 2 (15) and the telescopic rod of the square section of rotating shaft (36) rotate horizontally, allowing centrifugal chamber 13 to maintain a constant tilt angle during its outward expansion. This increases the centrifugal radius while maintaining the same tilt angle of centrifugal chamber 13.

[0120] Working Principle: The cell separation sampler of the present invention operates as follows: A No. 1 motor 2 is fixedly connected to the bottom end of the housing 1. Its output shaft is connected to a No. 1 bevel gear 3. A No. 2 bevel gear 6, which meshes vertically with the No. 1 motor, is fixedly connected to a No. 1 support rod 5 at its lower end. The No. 1 support rod 5 is rotationally connected to the housing 1 via a No. 1 bearing 4 at the bottom of the housing 1. The No. 2 bevel gear 6 has a fixed connection to a rotating shaft 36 at its upper end. The rotating shaft 36 has a square section at its upper end and a cylindrical section at its lower end. Four symmetrically distributed slots 17 are arranged along the axial direction of the cylindrical section.

[0121] The cylindrical section of the rotating shaft 36 is fitted with a No. 2 bearing 10. The four sliders 33 on the inner sidewall of the No. 2 bearing 10 correspond to the slide grooves 17, allowing the No. 2 bearing 10 to rotate synchronously with the rotating shaft 36 and slide up and down within the cylindrical section of the rotating shaft 36 via the sliders 33 and slide grooves 17. A rotating disk 39 is bolted above the No. 2 bearing 10, and a No. 1 rotating shaft 11 is provided at the upper end of the rotating disk 39. Four No. 2 rotating shafts 15 are provided on the sidewall of the square section of the rotating shaft 36. The four square centrifugal bins 13 are rotatably connected to the square end sidewalls of the rotating shaft 36 via the No. 2 rotating shafts 15. The No. 1 rotating shaft 11 at the lower end of the centrifugal bin 13 and the upper end of the rotating disk 39 are rotatably connected to the two ends of the four No. 2 support rods 9.

[0122] A threaded rod 16 is threadedly connected to the side wall of centrifugal chamber 13, with one end extending into the interior. This end is a plain, unthreaded shaft, connected to push plate 22 via bearing number 38. The shape and size of push plate 22 are adapted to the interior of centrifugal chamber 13, and the extended end of threaded rod 16 secures knob 14. The side wall of centrifugal chamber 13 and the other side of push plate 22 are covered with sponge board 23. A telescopic rod 7 is located at the other end of the bottom of housing 1. Above it, a support rod 12, number 3, runs parallel to the ground. Its center is fixed to the hydraulic rod of telescopic rod 7. One end is connected to the lower end of the outer ring of bearing number 2 10, and the other end is secured to fixed block 8.

[0123] When the centrifugation process is initiated, the control unit extends telescopic rod 7, pushing support rod 12 upward along the axis of rotation 36. Support rod 12 then moves the outer ring of bearing 10 upward. The inner ring of bearing 10 then moves rotating disk 39 up and down. Rotating disk 39 then drives support rod 9, causing the centrifuge chamber to rotate about axis 15. The tilt angle of centrifuge chamber 10 increases as the hydraulic rod of telescopic rod 7 is extended to meet varying centrifugation requirements. When the hydraulic rod retracts, centrifuge chamber 10 gradually returns to a horizontal position.

[0124] Furthermore, the outer ring of bearing No. 2 (10) is fixed to support rod No. 3 (12), ensuring stability. Lubricant is provided between the outer ring of bearing No. 2 (10) and rotating disk (39), providing support for rotating disk (39), ensuring stable rotation and thereby enhancing the stability of centrifugal chamber (13) during centrifugation. The design of rotating disk (39) not only enhances stability during centrifugation but also accelerates rotation when the centrifuge is stopped.

[0125] After the cell separation operation is complete, sampling begins. Manually adjust the centrifugal chamber 13 within the housing 1 to directly below the transport needle 18. Then, activate the second hydraulic press 21, pushing the transport needle 18 into the test tube in the centrifugal chamber 13. The photoelectric liquid level sensor 32 contains a built-in near-infrared light-emitting diode (LED) and a photosensor. Light from the LED is directed into the sensor's top lens. When the liquid submerges the lens, the light is refracted into the liquid, reducing the amount of light received by the receiver. This change in light is sensed by the sensor, which activates an electrical switch, controlling the external circuitry.

[0126] Due to the stratification of separated blood, different colored liquids absorb and refract light differently. When the sensor detects a significant change in light intensity, it determines its position and controls the transport needle 18 to stop descending. At this point, the three miniature second motors 26 are activated one at a time, controlling the bending needle 31 via the lead screw 28 to reach the sensor. The bent needle 31 falls to the liquid surface, affecting light refraction. The sensor's light intensity changes again, triggering an electrical switch to shut off the second motor 26 currently controlling the lead screw 28.

[0127] The photoelectric liquid level sensor offers accurate detection, fast response, and precise control, ensuring the tip of the bent needle 31 is precisely positioned at the interface between the two liquid levels. Once the upper layer of liquid is completely aspirated, the top of the sensor is free of liquid, and the light is completely absorbed by the receiver. The sensor then controls the transport needle to descend, repeating the process until all three layers of liquid are aspirated.

[0128] The basic principles, main features, and advantages of the present invention are shown and described above. Those skilled in the art should understand that the present invention is not limited to the foregoing embodiments. The foregoing embodiments and descriptions are merely illustrative of the principles of the present invention. Various changes and modifications may be made to the present invention without departing from the spirit and scope of the present invention. Such changes and modifications are intended to fall within the scope of the present invention. The scope of protection claimed in the present invention is defined by the appended claims and their equivalents.

Claims

1. A cell separation sampling instrument, characterized in that: Housing (1) A centrifugal assembly, wherein the centrifugal assembly is arranged in a housing (1), and the centrifugal assembly separates blood; the centrifugal assembly comprises a No. 1 telescopic rod (7), a No. 2 support rod (9) and a No. 3 support rod (12), wherein the hydraulic rod of the No. 1 telescopic rod (7) is fixedly connected to the No. 3 support rod (12), the No. 3 support rod (12) is connected to the outer ring of the No. 2 bearing (10), and a rotating disk (39) fixedly connected by bolts above the inner ring of the No. 2 bearing (10), the rotating disk (39) and the No. 2 support rod (9) are rotatably connected by a No. 1 rotating shaft (11), the end of the No. 2 support rod (9) is rotatably connected to the No. 1 rotating shaft (11) at the bottom of the centrifugal bin (13), and the centrifugal bin (13) is rotatably connected to the square section of the rotating shaft (36) by the No. 2 rotating shaft (15); A sampling component is arranged on the left side and above the shell (1), and the sampling component samples and collects the separated blood.

2. A cell separation sampling instrument according to claim 1, characterized in that: The centrifugal assembly includes a first motor (2) fixedly connected to the housing (1); A first bevel gear (3) is fixedly connected to the output shaft of the first motor (2); The second bevel gear (6) is vertically meshed with the first bevel gear (3); The upper end of the No. 1 support rod (5) is fixedly connected to the lower end of the No. 2 bevel gear (6), and the lower end of the No. 1 support rod (5) is rotatably connected to the bottom of the housing (1) through the No. 1 bearing (4); The rotating shaft (36) has an upper end configured as a square section and a lower end configured as a cylindrical section, and the lower end of the rotating shaft (36) is fixedly connected to the upper end of the second bevel gear (6); Slide grooves (17), wherein four of the slide grooves (17) are provided on the cylindrical section of the rotating shaft (36); The second bearing (10) is sleeved on the cylindrical section of the rotating shaft (36). The upper part of the inner ring of the second bearing (10) is fixedly connected to the rotating disk (39) by bolts. The upper part of the rotating disk (39) is rotatably connected to the first rotating shaft (11); Slide blocks 33, four of the slide blocks 33 are respectively arranged on the inner ring side wall of the No. 2 bearing 10, and the four slide blocks 33 slide in the four slide grooves 17 respectively; a No. 1 rotating shaft (11), the four No. 1 rotating shafts (11) are respectively rotatably connected to the upper end of the rotating disk (39); A second rotating shaft (15), wherein the four second rotating shafts (15) are rotatably connected to the four side walls of the square end of the rotating shaft (36); Centrifugal bins (13), wherein the four centrifugal bins (13) are rotatably connected to the rotating shaft (36) via the second rotating shaft (15), and the bottoms of the four centrifugal bins (13) are rotatably connected to a first rotating shaft (11); The two ends of the four No. 2 support rods (9) are respectively connected to the lower end of the centrifugal bin (13) and the No. 1 rotating shaft (11) on the upper end of the rotating disk (39) in a corresponding rotational manner; Threaded rods (16), wherein the four threaded rods (16) are respectively threadedly connected to the side walls of the centrifugal bin (13) and extend into the centrifugal bin (13), and one end of the threaded rod (16) extending into the centrifugal bin (13) is an optical axis; The push plate (22) is rotatably connected to one end of the optical axis of the threaded rod (16) extending into the centrifugal bin (13) via a No. 3 bearing (38); A knob (14) is fixedly connected to the other end of the threaded rod (16); A sponge plate (23) is provided on the other side of the side wall of the centrifugal bin (13) and the push plate (22); A first telescopic rod (7) is arranged at the bottom end of the inner portion of the housing (1); A third support rod (12) is arranged in parallel in the housing (1), and one end of the third support rod (12) is fixedly connected to the bottom of the outer ring of the second bearing (10). The third support rod (12) is fixedly connected to the hydraulic rod of the first telescopic rod (7) near the middle position. The first fixing block (8) is fixedly connected to the other end of the third support rod 12 and is used to balance the force on the third support rod 12.

3. A cell separation sampling instrument according to claim 1, characterized in that: The sampling assembly includes an aspirator (37) arranged on the side wall of the housing (1); A second hydraulic press (21) is provided on the side wall of the aspirator (37); The control chamber (19) is fixedly connected to the front end of the hydraulic rod of the second hydraulic press (21), and three circular holes (34) are opened at the lower end of the control chamber (19); No. 2 motor (26), three No. 2 motors (26) are arranged in the control compartment (19); Lead screws (28), the three lead screws (28) are fixedly connected to the output shafts of the three second motors (26), and the other ends extend out of the control compartment (19) through the circular hole (34); A transport needle (18) is fixedly connected to the lower end of the hydraulic rod of the second hydraulic press (21); Bending needles (31), three of the bending needles (31) are arranged in the transport needle (18), and the three bending needles (31) are respectively connected to the lead screw (28) through the threaded blocks (27) arranged thereon; Transport hoses (20), one end of the three transport hoses (20) is fixedly connected to the three bent needles (31) respectively, and the other end passes through the hole (35) on the control chamber (19) and is connected to the aspirator (37); The photoelectric liquid level sensor (32) is arranged at the bottom end of the inner side of the transport needle (18) and is connected to the three No. 2 motors (26) in the control chamber (19) through a data line (25) arranged in the side wall of the transport needle (18).

4. A cell separation sampling instrument according to claim 2, characterized in that: A plurality of loose elastic bands (24) are vertically fixedly connected in the centrifugal bin (13), and the width of the loose elastic bands (24) is the same as the height of the centrifugal bin (13).

5. The cell separation sampling instrument according to claim 3, characterized in that: The lower ends of the three lead screws (28) are respectively fixedly connected to a No. 2 fixing block (29).

6. A cell separation sampling instrument according to claim 3, characterized in that: The transport hose (20) is an elastic and retractable transport hose (20).

7. A cell separation sampling instrument according to claim 2, characterized in that: The push plate (22) is made of hard plastic.

8. The cell separation sampling instrument according to claim 4, characterized in that: A plurality of the loose elastic bands (24) are staggeredly connected in the centrifugal bin (13) and vertically fixedly connected in the centrifugal bin (13).

9. The cell separation sampling instrument according to claim 3, characterized in that: A baffle (30) is fixedly connected inside the transport needle (18); one end of the bending needle (31) passes through the baffle (30) and is sealed and slidably connected to the baffle (30).

10. The cell separation sampling instrument according to claim 2, characterized in that: The connection between the square section of the rotating shaft (36) and the second rotating shaft (15) can be configured as a telescopic rod.

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