Phosphorus nuclear magnetic resonance detection device, system and method and storage medium

Abstract

The invention discloses a phosphorus nuclear magnetic resonance detection device, system and method and a storage medium, and relates to the technical field of magnetic resonance detection. The phosphorus nuclear magnetic resonance detection device comprises a processor, the processor is configured to control a phosphorus coil to apply a target radio frequency signal to a phosphorus nucleus of a target to be detected, and the frequency of the target radio frequency signal is determined according to a target phosphorus-containing metabolite; and reading the phosphorus signal in the target phosphorus frequency range by using the target reading mode. The device indirectly reflects a signal of a certain marked phosphorus-containing metabolite by reading a total phosphorus signal, can amplify a signal of a phosphorus nucleus metabolite, improves the signal-to-noise ratio by several times, and improves the sensitivity of phosphorus nuclear magnetic resonance; meanwhile, due to the fact that the phosphorus spectrum is wide, metabolite is few, overlapping is few, and distinguishing is easy, only specific metabolite can be affected by applying the target radio-frequency signal, and the read phosphorus signal is high in interpretability.

Description

Technical Field

[0001] The present invention relates to the field of magnetic resonance detection technology, and in particular to a phosphorus nuclear magnetic resonance detection device, system, method and storage medium. Background Art

[0002] In recent years, chemical exchange saturation transfer (CEST) magnetic resonance imaging is an emerging molecular imaging technology that can be used for metabolic detection of diseases such as cancer, tumors, and stroke, as well as pH detection. Its signal source is hydrogen nuclei, and the principle is as follows: the frequency of hydrogen protons in metabolites such as proteins, glucose, creatine, and salicylic acid analogs in the human body is different from the frequency of water hydrogen protons, and the hydrogen protons in these metabolites can be exchanged with water hydrogen protons. Therefore, by applying saturation radio frequency pulses of a specific frequency to human tissue, the hydrogen protons in these metabolites can reach a saturated state, and then affect the water signal intensity through the exchange effect. By detecting changes in the water signal intensity, the exchange rate and concentration information of various metabolites in the body can be indirectly reflected. Therefore, CEST technology can be used as a signal amplifier for low-concentration biological molecules. Usually, CEST requires the acquisition of magnetic resonance (MR) images under the action of saturation pulses of different frequencies to reflect the metabolite information at the corresponding frequency.

[0003] Due to the limitations of CEST technology (it cannot measure functional groups that do not contain hydrogen), related technologies have proposed the use of phosphorus nuclear magnetic resonance to non-invasively detect the concentration of phosphorus-containing metabolites in living bodies, such as high-energy phosphates (creatine phosphate, adenosine triphosphate ATP, inorganic phosphorus), phospholipids, etc., as well as to detect pH and magnesium ion concentration, thereby providing important metabolic information.

[0004] However, phosphorus NMR has the following challenges: 1) The sensitivity of phosphorus nuclei themselves is low (only 6.7% of that of hydrogen nuclei); 2) The concentration of phosphorus-containing metabolites in the body is low (all on the order of several mM, while the concentration of hydrogen nuclei is 110M); 3) The T2 of phosphorus-containing metabolites is short, which results in a poor signal-to-noise ratio for this technology. In addition, most phosphorus NMR technologies are phosphorus-31Magnetic Resonance Spectrum Imaging (31P-MRSI), which requires the acquisition of four-dimensional magnetic resonance data (three-dimensional physical space + frequency dimension). By analyzing the phosphorus spectrum information in the frequency dimension, the concentration information of the metabolites is obtained, and then the spatial distribution is obtained. However, due to the poor signal-to-noise ratio of phosphorus NMR, larger imaging voxels and multiple averaging are usually required, which results in poor spatiotemporal resolution of the technology, seriously limiting the development of phosphorus NMR. Therefore, how to improve the sensitivity of phosphorus NMR is an important issue that needs to be solved in this field. Summary of the Invention

[0005] The present invention aims to at least partially address one of the technical problems in the related art. To this end, the present invention provides a phosphorus nuclear magnetic resonance detection device, system, method, and storage medium to amplify the signal of phosphorus nuclear metabolites, improve the sensitivity of phosphorus nuclear magnetic resonance, and enhance the interpretability of the signal.

[0006] In a first aspect, an embodiment of the present invention provides a phosphorus nuclear magnetic resonance detection device, comprising a processor, wherein the processor is configured to: control a phosphorus coil to apply a target radio frequency signal to the phosphorus nuclei of a target to be detected, wherein the frequency of the target radio frequency signal is determined according to the target phosphorus-containing metabolite; and read the phosphorus signal within the target phosphorus frequency range using a target reading method.

[0007] In addition, the phosphorus nuclear magnetic resonance detection device according to the embodiment of the present invention may also have the following additional technical features:

[0008] According to one embodiment of the present invention, the frequency of the target radio frequency signal is determined according to a preset center frequency and a target offset frequency, wherein the target offset frequency is the frequency difference between the chemical shift of the target phosphorus-containing metabolite and the preset center frequency.

[0009] According to one embodiment of the present invention, the preset center frequency is the Larmor frequency of the phosphorus core.

[0010] According to an embodiment of the present invention, the target radio frequency signal adopts a continuous wave pulse or a pulse train.

[0011] According to one embodiment of the present invention, the target reading mode includes at least one of: image-only reading, non-position-selective spectral reading, position-selective spectral reading, and spectral imaging reading.

[0012] According to one embodiment of the present invention, when the target reading method adopts the image-only reading method, the processor is specifically configured to: apply a magnetic field gradient in any direction in three-dimensional space for spatial encoding when reading the phosphorus signal within the target phosphorus frequency range using the target reading method; and read the phosphorus signal within the target phosphorus frequency range when the gradient is turned on, wherein the target phosphorus frequency range includes the preset center frequency.

[0013] In a second aspect, an embodiment of the present invention provides a phosphorus nuclear magnetic resonance detection system, comprising: a host, a radio frequency module, and a phosphorus coil, wherein the host is configured to: control the radio frequency module to transmit a target radio frequency signal to the phosphorus coil, so as to apply the target radio frequency signal to the phosphorus nucleus of the target to be measured through the phosphorus coil, wherein the frequency of the target radio frequency signal is determined according to the target phosphorus-containing metabolite; receive the magnetic resonance signal generated by the target to be measured fed back by the phosphorus coil through the radio frequency module, and read the magnetic resonance signal using a target reading method to obtain a phosphorus signal within the target phosphorus frequency range.

[0014] In addition, the phosphorus nuclear magnetic resonance detection system of the embodiment of the present invention may also have the following additional technical features:

[0015] According to one embodiment of the present invention, the frequency of the target radio frequency signal is determined based on a preset center frequency and a target offset frequency, wherein the target offset frequency is the frequency difference between the chemical shift of the target phosphorus-containing metabolite and the preset center frequency.

[0016] In a third aspect, an embodiment of the present invention provides a phosphorus nuclear magnetic resonance detection method, comprising: applying a target radio frequency signal to the phosphorus nuclei of the target to be detected through a phosphorus coil, wherein the frequency of the target radio frequency signal is determined according to the target phosphorus-containing metabolite; and reading the phosphorus signal within the target phosphorus frequency range using a target reading method.

[0017] In addition, the phosphorus nuclear magnetic resonance detection method according to the embodiment of the present invention may also have the following additional technical features:

[0018] According to one embodiment of the present invention, the frequency of the target radio frequency signal is determined according to a preset center frequency and a target offset frequency, wherein the target offset frequency is the frequency difference between the chemical shift of the target phosphorus-containing metabolite and the preset center frequency.

[0019] According to one embodiment of the present invention, the preset center frequency is determined according to the Larmor frequency of the phosphorus core.

[0020] According to an embodiment of the present invention, the target radio frequency signal adopts a continuous wave pulse or a pulse train.

[0021] According to one embodiment of the present invention, the target reading mode includes at least one of: image-only reading, non-position-selective spectral reading, position-selective spectral reading, and spectral imaging reading.

[0022] According to one embodiment of the present invention, when the target reading method adopts the image-only reading method, the use of the target reading method to read the phosphorus signal within the target phosphorus frequency range includes: applying a magnetic field gradient in any direction in three-dimensional space for spatial encoding; and reading the phosphorus signal within the target phosphorus frequency range when the gradient is turned on, wherein the target phosphorus frequency range includes a preset center frequency.

[0023] In a fourth aspect, an embodiment of the present invention provides a computer-readable storage medium having a computer program stored thereon. When the computer program is executed by a processor, the phosphorus nuclear magnetic resonance detection method described in the third aspect is implemented.

[0024] The phosphorus nuclear magnetic resonance detection device, system, method, and storage medium of the embodiments of the present invention first apply a target radio frequency signal to the phosphorus nucleus of the target to be detected through a phosphorus coil. The frequency of the target radio frequency signal is determined according to the target phosphorus-containing metabolite. Then, a target reading method is used to read the phosphorus signal within the target phosphorus frequency range. Thus, by reading the overall phosphorus signal to indirectly reflect the signal of a certain labeled phosphorus-containing metabolite, the signal of the phosphorus nuclear metabolite can be amplified, the signal-to-noise ratio can be increased by several times, and the sensitivity of phosphorus nuclear magnetic resonance can be improved. At the same time, because the phosphorus spectrum is wide and there are fewer metabolites, there is less overlap and it is easy to distinguish. Therefore, applying the target radio frequency signal will only affect specific metabolites, making the read phosphorus signal highly interpretable.

[0025] Additional aspects and advantages of the present invention will be set forth in part in the description which follows and, in part, will be obvious from the description which follows, or may be learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 is a structural block diagram of a phosphorus nuclear magnetic resonance detection device according to an embodiment of the present invention;

[0027] Figure 2 is a structural block diagram of a phosphorus nuclear magnetic resonance detection system according to an embodiment of the present invention;

[0028] Figure 3 is a spectrum of phosphorus-containing metabolites according to an embodiment of the present invention;

[0029] Figure 4 is a schematic diagram of a target radio frequency signal according to an embodiment of the present invention;

[0030] Figure 5 is a schematic diagram of a target radio frequency signal according to another embodiment of the present invention;

[0031] Figure 6 is a schematic diagram of phosphorus signal reading according to one embodiment of the present invention;

[0032] Figure 7 is a structural block diagram of a host according to an embodiment of the present invention;

[0033] Figure 8 4 is a flow chart of a phosphorus nuclear magnetic resonance detection method according to an embodiment of the present invention.

[0034] Reference numerals:

[0035] Phosphorus nuclear magnetic resonance detection system 1000, phosphorus nuclear magnetic resonance detection device 100, processor 10, host 1, radio frequency module 2, phosphorus coil 3, controller 501, bus 502, memory 503, transceiver 504. DETAILED DESCRIPTION

[0036] The following describes embodiments of the present invention in detail, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present invention, and are not to be construed as limiting the present invention.

[0037] The following describes a phosphorus nuclear magnetic resonance detection device, system, method, and storage medium according to embodiments of the present invention with reference to the accompanying drawings.

[0038] The present invention introduces CEST technology from traditional hydrogen nuclear magnetic resonance to phosphorus nuclear magnetic resonance to amplify the signal of phosphorus nuclear metabolites and improve the sensitivity of phosphorus nuclear magnetic resonance. The specific process is as follows: the detection process includes two stages: labeling and reading. In the labeling stage, a target radio frequency signal with a specific frequency (determined according to the target phosphorus-containing metabolite) is applied to label the phosphorus nucleus in the target phosphorus-containing metabolite. This saturated signal causes the overall phosphorus signal to change through phosphate group exchange and phosphorus chemical reaction; in the reading stage, the target reading method is used to detect the change in the phosphorus signal to indirectly reflect the concentration information of the labeled target phosphorus-containing metabolite and its mutual conversion characteristics.

[0039] Figure 1 4 is a structural block diagram of a phosphorus nuclear magnetic resonance detection device according to an embodiment of the present invention.

[0040] like Figure 1 As shown, the phosphorus nuclear magnetic resonance detection device 100 includes a processor 10, which is configured to: control the phosphorus coil to apply a target radio frequency signal to the phosphorus nuclei of the target to be detected, wherein the frequency of the target radio frequency signal is determined according to the target phosphorus metabolite; use a target reading method to read the phosphorus signal within the target phosphorus frequency range

[0041] In embodiments of the present invention, the phosphorus coil can be a single phosphorus coil or a multi-element coil comprising the phosphorus coil, such as a hydrogen-phosphorus dual-frequency coil. The phosphorus coil is a device capable of generating a magnetic field based on a target radio frequency signal to act on the target under test. Under the influence of the magnetic field, the phosphorus nuclei in the target under test transition from a low-energy state to a high-energy state. When the magnetic field generated by the phosphorus coil disappears, the phosphorus nuclei subjected to the magnetic field emit magnetic resonance signals, simultaneously transitioning from a high-energy state to a low-energy state. At this point, the phosphorus coil can receive the magnetic resonance signals emitted by the phosphorus nuclei in the target under test.

[0042] like Figure 2As shown, during phosphorus nuclear magnetic resonance detection, host 1 controls radio frequency module 2 (a component of the magnetic resonance device, which may include a radio frequency signal generator, a radio frequency signal amplifier, etc.) to transmit a target radio frequency signal to phosphorus coil 3, so that the target radio frequency signal is applied to the phosphorus nuclei of the target to be detected through phosphorus coil 3. Subsequently, radio frequency module 1 receives the magnetic resonance signal generated by the target to be detected, which is fed back by phosphorus coil 3, and reads the magnetic resonance signal using a target reading method to obtain a phosphorus signal within the target phosphorus frequency range.

[0043] Exemplarily, the host 1 can control the RF module 2 to transmit a target RF signal based on a target marker sequence, wherein the target marker sequence can be generated by the host or input by the user. For example, the host 1 can provide an operation interaction interface, and the user can create or select a target marker sequence. The host 1 can generate a RF control word (including fields such as RF excitation frequency, RF frequency width, RF pulse excitation duration, and RF pulse waveform) based on the target marker sequence, and use the RF control word to control the RF module 2 to transmit the target RF signal to drive the phosphorus coil 3 to generate a spatial magnetic field, so that the imaging voxels in the imaging space absorb the RF energy in the space to resonate. The phosphorus coil 3 sends the received magnetic resonance signal to the RF module 2, which is processed by the RF module 2 such as analog-to-digital conversion, filtering, and amplification, and then transmitted to the host 1 for reading.

[0044] In an embodiment of the present invention, the target to be measured can be stably fixed on a workbench of a magnetic resonance device (which may include a radio frequency module 2) and transported to a detection position of the magnetic resonance device. The target to be measured can include a human, an animal, or a part of a human or an animal. For example, the target to be measured can include an internal organ (e.g., the liver, heart, uterus, brain, breast, abdomen, etc.) or a blood vessel.

[0045] In some embodiments of the present invention, the frequency of the target radio frequency signal is determined based on a preset center frequency and a target deviation frequency, wherein the target deviation frequency is the frequency difference between the chemical shift of the target phosphorus-containing metabolite and the preset center frequency, and the preset center frequency is determined based on the Larmor frequency of the phosphorus nucleus.

[0046] like Figure 3As shown, the horizontal axis represents the position of each phosphorus-containing metabolite (including phosphatidylethanolamine PE, phosphatidylcholine PC, glycerophosphocholine GPC, glycerophosphatidylethanolamine GPE, phosphocreatine PCr, γ-adenosine triphosphate ATP, αATP, βATP, nicotinamide adenine dinucleotide NAD, diphosphate glucose UDPG) during resonance, and the vertical axis represents the content of each phosphorus-containing metabolite. The target phosphorus-containing metabolite can also be defined as a phosphorus-containing metabolite of interest. The target phosphorus-containing metabolite is any one of PE, PC, GPC, GPE, γATP, αATP, βATP, NAD, and UDPG, which can be selected as needed. For example, the target phosphorus-containing metabolite is PE. The preset center frequency can also be defined as the fundamental frequency ω0, which can be determined based on the phosphorus nucleus Larmor frequency, for example, the phosphorus nucleus Larmor frequency (17.2 MHz / T*main magnetic field strength B0), which usually coincides with the highest peak phosphocreatine PCr frequency in tissues.

[0047] As an implementation manner, the frequency of the target radio frequency signal is the sum of a preset center frequency and a target offset frequency.

[0048] Specifically, after identifying the target phosphorus-containing metabolite, the target deviation frequency can be determined, and then the following can be obtained: the frequency of the target RF signal = the preset center frequency + the target deviation frequency, that is, the operating frequency = ω0 + Δω, where Δω is the target deviation frequency, which is the difference between the chemical shift of the target phosphorus-containing metabolite (in ppm, a priori knowledge) and the frequency of PCr. Of course, the chemical shift of creatine phosphate PCr is 0 ppm. A narrowband RF pulse (i.e., the target RF signal) can then be applied through the phosphorus coil to mark the phosphorus signal at a specific frequency.

[0049] In some embodiments of the present invention, Figure 4 As shown, the target RF signal may be a continuous wave pulse or a pulse train, wherein the specific pulse waveform of the pulse train includes but is not limited to a hard pulse, a Gaussian pulse, a Fermi pulse, an SLR (Shinnar-Le Roux) pulse, etc. The intensity and duration of the target RF signal may be set as needed.

[0050] For example, Figure 5 As shown, the target RF signal may also include a spoiler gradient pulse. The intensity of the continuous wave pulse or pulse train is less than that of the spoiler gradient pulse, and the duration is greater than that of the spoiler gradient pulse. The provision of the spoiler gradient pulse can destroy the residual transverse magnetization to avoid interference with subsequent signal acquisition.

[0051] In embodiments of the present invention, the target phosphorus frequency range can be determined as needed and can be >1 ppm, for example, 10-30 ppm, or cover the entire phosphorus spectrum to achieve signal amplification. Target reading methods can include at least one of image-only reading, non-position-selective spectral reading, position-selective spectral reading, and spectral imaging reading.

[0052] Among them, the image-only reading method can achieve fast, high-resolution anatomical imaging by optimizing sequence parameters, spatial encoding strategies, etc. The non-position-selective spectrum reading method can only use radio frequency pulses (such as 90° hard pulses) for excitation without applying any gradient field. It has a high signal-to-noise ratio, a simple sequence, and a short scanning time. The position-selective spectrum reading method selectively excites signals in specific areas through a combination of gradient fields and radio frequency pulses. Its spatial positioning is precise, which can avoid interference from surrounding tissues and can quantitatively analyze metabolite concentrations. The positioning technologies used include free induction decay (FID), image-selected in vivo spectroscopy (ISIS), and stimulated echo acquisition mode (STEAM). The spectrum imaging reading method requires spatial encoding and frequency resolution, and can simultaneously acquire spectral lines of multiple voxels to generate metabolite distribution maps and realize the visualization of the spatial distribution of metabolites.

[0053] Exemplarily, when the target reading method adopts the image-only reading method, the target reading method is used to read the phosphorus signal within the target phosphorus frequency range, including: applying a magnetic field gradient in any direction in three-dimensional space for spatial encoding; reading the phosphorus signal within the target phosphorus frequency range when the gradient is turned on, wherein the target phosphorus frequency range includes the preset center frequency.

[0054] like Figure 6 As shown, Figure 6 (a) and (b) show the readout sequence and K-space data acquisition used in the 31P-MRSI method. Figure 6 (c) and (d) show the readout sequence and K-space data acquisition used in the image-only readout method adopted by the present invention (taking the application of a magnetic field gradient in the x-direction in three-dimensional space for spatial encoding as an example). The 31P-CEST readout method acquires one point at a time, and the number of K-space acquisitions is Nx×Ny; while the image-only readout method in the present invention acquires one line at a time, and the number of K-space acquisitions is Ny, where Nx and Ny are the number of horizontal and vertical points in K-space, respectively. It can be seen from this that the present invention can convert Gx from phase encoding to frequency encoding after applying a target radio frequency signal for phosphorus nucleus labeling, thereby reducing one phase encoding direction and achieving acquisition dimensionality reduction.

[0055] Specifically, as an embodiment, for image-only readout, a single pixel bandwidth (ie, target phosphor frequency range) may cover the entire phosphor spectrum. Figure 6 In (c) and (d), a Gx magnetic field gradient is applied in the x-direction for spatial encoding. When the gradient is on, the phosphorus signal is read across the entire phosphorus spectrum. This readout process does not involve frequency resolution, but only spatial encoding. Compared to traditional spectral imaging (31P-MRSI), phase encoding in the x-direction is omitted, reducing the acquisition time to 1 / Nx of the original. Furthermore, because the single-voxel bandwidth covers the entire phosphorus spectrum, signal amplification is achieved.

[0056] The invention also provides a phosphorus nuclear magnetic resonance detection system.

[0057] like Figure 2 As shown, the phosphorus nuclear magnetic resonance detection system 1000 includes: a host 1, a radio frequency module 2, and a phosphorus coil 3. The host 1 is used to: generate a target marker sequence, and control the radio frequency module to transmit a target radio frequency signal to the phosphorus coil based on the target marker sequence, so as to apply the target radio frequency signal to the phosphorus nucleus of the target to be measured through the phosphorus coil, wherein the frequency of the target radio frequency signal is determined according to the target phosphorus-containing metabolite; receive the magnetic resonance signal generated by the target to be measured fed back by the phosphorus coil through the radio frequency module, and read the magnetic resonance signal using a target reading method to obtain a phosphorus signal within the target phosphorus frequency range.

[0058] For example, the radio frequency module 2 and the phosphorus coil 3 may be integrated, for example, both may be components of a magnetic resonance device.

[0059] In some embodiments of the present invention, the frequency of the target radio frequency signal is determined based on a preset center frequency and a target offset frequency, wherein the target offset frequency is the frequency difference between the chemical shift of the target phosphorus-containing metabolite and the preset center frequency.

[0060] Exemplarily, the preset center frequency is the phosphorus core Larmor frequency.

[0061] In some embodiments of the present invention, the target radio frequency signal adopts a continuous wave pulse or a pulse train.

[0062] In some embodiments of the present invention, the target reading method includes at least one of: image-only reading, non-position-selective spectral reading, position-selective spectral reading, and spectral imaging reading.

[0063] Exemplarily, when the target reading method adopts the image-only reading method, when the host 1 uses the target reading method to read the phosphorus signal within the target phosphorus frequency range, it is specifically configured as follows: applying a magnetic field gradient in any direction in three-dimensional space for spatial encoding; reading the phosphorus signal within the target phosphorus frequency range when the gradient is turned on, wherein the target phosphorus frequency range includes a preset center frequency.

[0064] Figure 7 It is a structural block diagram of a host according to an embodiment of the present invention.

[0065] like Figure 7 As shown, host 1 includes a controller 501 and a memory 503. Controller 501 and memory 503 are connected, for example, via a bus 502. Optionally, host 1 may also include a transceiver 504. It should be noted that in practical applications, the number of transceivers 504 is not limited to one, and the structure of host 1 does not constitute a limitation on the embodiments of the present invention.

[0066] The controller 501 may be a CPU (Central Processing Unit), a general-purpose processor, a DSP (Digital Signal Processor), an ASIC (Application Specific Integrated Circuit), an FPGA (Field Programmable Gate Array), or other programmable logic devices, transistor logic devices, hardware components, or any combination thereof. It may implement or execute the various exemplary logic blocks, modules, and circuits described in conjunction with the present disclosure. The controller 501 may also be a combination that implements computing functions, such as a combination of one or more microprocessors, a combination of a DSP and a microprocessor, and the like.

[0067] The bus 502 may include a path for transmitting information between the above components. The bus 502 may be a PCI (Peripheral Component Interconnect) bus or an EISA (Extended Industry Standard Architecture) bus. The bus 502 may be divided into an address bus, a data bus, a control bus, etc. For ease of representation, Figure 7 Only one thick line is used in the diagram, but this does not mean that there is only one bus or one type of bus.

[0068] The memory 503 is used to store a computer program corresponding to the phosphorus nuclear magnetic resonance detection method of the following embodiment of the present invention, and the computer program is controlled and executed by the controller 501. The controller 501 is used to execute the computer program stored in the memory 503 to implement the content shown in the above method embodiment.

[0069] The host 1 includes but is not limited to fixed terminals such as desktop computers. Figure 7 The host 1 shown is only an example and should not bring any limitation to the functions and scope of use of the embodiments of the present invention.

[0070] It should be noted that, for other specific implementations of the phosphorus nuclear magnetic resonance detection system 1000 according to the embodiment of the present invention, reference may be made to the specific implementations of the phosphorus nuclear magnetic resonance detection device 100 according to the above embodiment.

[0071] Figure 8 4 is a flow chart of a phosphorus nuclear magnetic resonance detection method according to an embodiment of the present invention.

[0072] like Figure 8 As shown, the phosphorus nuclear magnetic resonance detection method includes:

[0073] S11, applying a target radio frequency signal to the phosphorus nuclei of the target to be measured through the phosphorus coil, wherein the frequency of the target radio frequency signal is determined according to the target phosphorus-containing metabolite.

[0074] In some embodiments of the present invention, the frequency of the target RF signal is determined based on a preset center frequency and a target offset frequency. For example, the frequency of the target RF signal is the sum of the preset center frequency and the target offset frequency. The target offset frequency is the frequency difference between the chemical shift of the target phosphorus-containing metabolite and the preset center frequency. The preset center frequency can also be defined as the fundamental frequency ω0, which is determined based on the Larmor frequency of the phosphorus nucleus. For example, the preset center frequency is the Larmor frequency of the phosphorus nucleus (17.2 MHz / T*main magnetic field strength B0), which generally coincides with the highest peak frequency of phosphocreatine PCr in tissues.

[0075] After identifying the target phosphorus-containing metabolite, the target deviation frequency can be determined. This yields the following: target RF signal frequency = preset center frequency + target deviation frequency. This means operating frequency = ω0 + Δω, where Δω is the target deviation frequency, representing the difference between the chemical shift of the target phosphorus-containing metabolite (in ppm, a priori knowledge) and the frequency of PCr. Naturally, the chemical shift of creatine phosphate PCr is 0 ppm. A narrowband RF pulse (i.e., the target RF signal) can then be applied through the phosphorus coil to target the phosphorus signal at a specific frequency.

[0076] In some embodiments of the present invention, Figure 4 As shown, the target RF signal may be a continuous wave pulse or a pulse train, wherein the specific pulse waveform of the pulse train includes but is not limited to a hard pulse, a Gaussian pulse, a Fermi pulse, an SLR (Shinnar-Le Roux) pulse, etc. The intensity and duration of the target RF signal may be set as needed.

[0077] For example, Figure 5 As shown, the target RF signal may also include a spoiler gradient pulse. The intensity of the continuous wave pulse or pulse train is less than that of the spoiler gradient pulse, and the duration is greater than that of the spoiler gradient pulse. The provision of the spoiler gradient pulse can destroy the residual transverse magnetization to avoid interference with subsequent signal acquisition.

[0078] S12, reading the phosphorus signal within the target phosphorus frequency range using a target reading method.

[0079] The target phosphorus frequency range can be determined as needed, and can be >1 ppm, such as 10-30 ppm, or cover the entire phosphorus spectrum to achieve signal amplification.

[0080] In an embodiment of the present invention, the target reading method includes at least one of: image-only reading, non-position-selective spectral reading, position-selective spectral reading, and spectral imaging reading.

[0081] Exemplarily, when the target reading method adopts the image-only reading method, the target reading method is used to read the phosphorus signal within the target phosphorus frequency range, including: applying a magnetic field gradient in any direction in three-dimensional space for spatial encoding; reading the phosphorus signal within the target phosphorus frequency range when the gradient is turned on, wherein the target phosphorus frequency range includes the preset center frequency.

[0082] It should be noted that, for other specific implementations of the phosphorus nuclear magnetic resonance detection method according to the embodiment of the present invention, reference may be made to the specific implementations of the phosphorus nuclear magnetic resonance detection device 100 according to the above embodiment.

[0083] In summary, the phosphorus nuclear magnetic resonance detection device, system, method, and storage medium of the embodiments of the present invention have the following advantages:

[0084] 1) By detecting the overall phosphorus signal, which indirectly reflects the signal of a specific phosphorus-containing metabolite, the signal-to-noise ratio can be increased several times (the proportion of phosphorus-containing metabolites in different parts is different, so the signal-to-noise ratio amplification factor is slightly different);

[0085] 2) Convert the frequency dimension in traditional 31P-MRSI four-dimensional acquisition to the frequency of the CEST saturation pulse, and then acquire the total phosphorus signal to indirectly detect the corresponding phosphorus-containing metabolites. Specifically, based on the MRS / MRI acquisition method, the center frequency of the radiofrequency pulse is adjusted to 17.2×B0 MHz to match the Larmor frequency of 31P. Combined with gradient events such as slice selection, phase encoding, and frequency encoding, imaging encoding of the spatial dimension is achieved. During signal readout, a readout gradient is set in a certain direction, and K-space is filled in a linear manner. This reduces the acquisition dimension by one, shortens the acquisition time exponentially, and is easy to implement.

[0086] 3) Compared with hydrogen nucleus CEST, which has the problem of difficult signal interpretation (the hydrogen spectrum is narrow ([0-8] ppm), while there are many metabolites, there is serious overlap, and applying a CEST pulse will affect multiple metabolites, resulting in poor interpretability of the final signal), the phosphorus spectrum used in the present invention is wider ([-20-10] ppm), and there are fewer metabolites, with less overlap and easier to distinguish. Applying a CEST pulse will only affect specific metabolites, and the final signal is highly interpretable.

[0087] It should be noted that the logic and / or steps represented in the flowcharts or otherwise described herein, for example, can be considered as a sequenced list of executable instructions for implementing the logical functions, and can be embodied in any computer-readable medium for use by an instruction execution system, apparatus, or device (such as a computer-based system, a system including a processor, or other system that can fetch and execute instructions from an instruction execution system, apparatus, or device), or in conjunction with such instruction execution system, apparatus, or device. For the purposes of this specification, a "computer-readable medium" can be any device that can contain, store, communicate, propagate, or transmit a program for use by an instruction execution system, apparatus, or device, or in conjunction with such instruction execution system, apparatus, or device. More specific examples (non-exhaustive list) of computer-readable media include the following: an electrical connection portion having one or more wires (electronic device), a portable computer disk cartridge (magnetic device), random access memory (RAM), read-only memory (ROM), erasable and programmable read-only memory (EPROM or flash memory), fiber optic devices, and portable compact disk read-only memory (CDROM). Furthermore, the computer-readable medium may even be paper or other suitable medium on which the program is printed, since the program may be obtained electronically, for example, by optically scanning the paper or other medium and then editing, interpreting or processing it in another suitable manner if necessary, and then storing it in a computer memory.

[0088] It should be understood that various parts of the present invention can be implemented using hardware, software, firmware, or a combination thereof. In the above-described embodiments, multiple steps or methods can be implemented using software or firmware stored in a memory and executed by a suitable instruction execution system. For example, if implemented using hardware, as in another embodiment, any one of the following technologies known in the art or a combination thereof can be used: a discrete logic circuit having a logic gate circuit for implementing a logic function on a data signal, an application-specific integrated circuit having a suitable combination of logic gate circuits, a programmable gate array (PGA), a field programmable gate array (FPGA), etc.

[0089] Throughout this specification, reference to terms such as "one embodiment," "some embodiments," "examples," "specific examples," or "some examples" means that a specific feature, structure, material, or characteristic described in conjunction with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

[0090] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like to indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0091] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of the technical features being referred to. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "plurality" means at least two, such as two, three, etc., unless otherwise specifically defined.

[0092] In the present invention, unless otherwise specified or limited, the terms "installed," "connected," "connect," "fixed," etc. should be understood in a broad sense. For example, they can refer to fixed connection, detachable connection, or integration; mechanical connection, electrical connection; direct connection, or indirect connection through an intermediate medium; internal communication between two components, or interaction between two components, unless otherwise specified. Those skilled in the art will understand the specific meanings of the above terms in the present invention based on specific circumstances.

[0093] In the present invention, unless otherwise expressly specified or limited, when a first feature is "above" or "below" a second feature, it may mean that the first and second features are in direct contact, or that the first and second features are in indirect contact through an intermediary. Furthermore, when a first feature is "above," "above," or "above" a second feature, it may mean that the first feature is directly above or diagonally above the second feature, or simply means that the first feature is at a higher level than the second feature. When a first feature is "below," "below," or "below" a second feature, it may mean that the first feature is directly below or diagonally below the second feature, or simply means that the first feature is at a lower level than the second feature.

[0094] Although the embodiments of the present invention have been shown and described above, it will be understood that the above embodiments are illustrative and are not to be construed as limitations on the present invention. A person skilled in the art may change, modify, replace and modify the above embodiments within the scope of the present invention.

Claims

1. A phosphorus nuclear magnetic resonance detection device, characterized in that: comprising a processor configured to: controlling the phosphorus coil to apply a target radio frequency signal to the phosphorus nuclei of the target to be measured, wherein the frequency of the target radio frequency signal is determined according to the target phosphorus-containing metabolite; The target reading mode is used to read the phosphorus signal within the target phosphorus frequency range.

2. The phosphorus nuclear magnetic resonance detection device according to claim 1, characterized in that The frequency of the target radio frequency signal is determined according to a preset center frequency and a target offset frequency, wherein the target offset frequency is a frequency difference between the chemical shift of the target phosphorus-containing metabolite and the preset center frequency.

3. The phosphorus nuclear magnetic resonance detection device according to claim 2, characterized in that: The preset center frequency is determined according to the Larmor frequency of the phosphorus core.

4. The phosphorus nuclear magnetic resonance detection device according to any one of claims 1 to 3, characterized in that: The target radio frequency signal adopts continuous wave pulse or pulse train.

5. The phosphorus nuclear magnetic resonance detection device according to any one of claims 1 to 3, characterized in that: The target reading method includes at least one of image-only reading, non-position-selective spectral reading, position-selective spectral reading, and spectral imaging reading.

6. The phosphorus nuclear magnetic resonance detection device according to claim 5, characterized in that: When the target reading mode adopts the image-only reading mode, the processor is specifically configured to read the phosphorus signal within the target phosphorus frequency range using the target reading mode: Applying magnetic field gradients in any direction in three-dimensional space for spatial encoding; When the gradient is turned on, a phosphorus signal within the target phosphorus frequency range is read, wherein the target phosphorus frequency range includes a preset center frequency.

7. A phosphorus nuclear magnetic resonance detection system, characterized in that: include: Host, radio frequency module, phosphorus coil, the host is configured as: controlling the radio frequency module to transmit a target radio frequency signal to the phosphorus coil, so as to apply the target radio frequency signal to the phosphorus nuclei of the target to be measured through the phosphorus coil, wherein the frequency of the target radio frequency signal is determined according to the target phosphorus-containing metabolite; The radio frequency module receives the magnetic resonance signal generated by the target to be measured and fed back by the phosphorus coil, and reads the magnetic resonance signal using a target reading method to obtain a phosphorus signal within a target phosphorus frequency range.

8. The phosphorus nuclear magnetic resonance detection system according to claim 7, characterized in that: The frequency of the target radio frequency signal is determined based on a preset center frequency and a target offset frequency, wherein the target offset frequency is a frequency difference between the chemical shift of the target phosphorus-containing metabolite and the preset center frequency.

9. A phosphorus nuclear magnetic resonance detection method, characterized in that: include: applying a target radio frequency signal to the phosphorus nuclei of the target to be measured through a phosphorus coil, wherein the frequency of the target radio frequency signal is determined according to the target phosphorus-containing metabolite; The target reading mode is used to read the phosphorus signal within the target phosphorus frequency range.

10. The phosphorus nuclear magnetic resonance detection method according to claim 9, characterized in that: The frequency of the target radio frequency signal is determined according to a preset center frequency and a target offset frequency, wherein the target offset frequency is a frequency difference between the chemical shift of the target phosphorus-containing metabolite and the preset center frequency.

11. The phosphorus nuclear magnetic resonance detection method according to claim 10, characterized in that: The preset center frequency is determined according to the Larmor frequency of the phosphorus core.

12. The phosphorus nuclear magnetic resonance detection method according to any one of claims 9 to 11, characterized in that: The target radio frequency signal adopts continuous wave pulse or pulse train.

13. The phosphorus nuclear magnetic resonance detection method according to any one of claims 9 to 11, characterized in that: The target reading method includes at least one of image-only reading, non-position-selective spectral reading, position-selective spectral reading, and spectral imaging reading.

14. The phosphorus nuclear magnetic resonance detection method according to claim 13, characterized in that: When the target reading method adopts the image-only reading method, the method of reading the phosphorus signal within the target phosphorus frequency range by using the target reading method includes: Applying magnetic field gradients in any direction in three-dimensional space for spatial encoding; When the gradient is turned on, a phosphorus signal within the target phosphorus frequency range is read, wherein the target phosphorus frequency range includes a preset center frequency.

15. A computer-readable storage medium having a computer program stored thereon, characterized in that: When the computer program is executed by a processor, the phosphorus nuclear magnetic resonance detection method according to any one of claims 9 to 14 is implemented.

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