Anti-freezing agent for grapes and preparation method of anti-freezing agent

Abstract

The invention discloses an antifreezing agent for grapes and a preparation method of the antifreezing agent, and belongs to the technical field of crop antifreezing. The anti-freezing agent provided by the invention comprises the following components: brassinolide, compound sodium nitrophenolate, abscisic acid and small molecule peptide in a mass ratio of 0.26: 60: 35: 650, and after the anti-freezing agent is prepared into a preparation, the concentration of the brassinolide is 0.26 mg / L, the concentration of the compound sodium nitrophenolate is 60 mg / L, the concentration of the abscisic acid is 35 mg / L, and the concentration of the small molecule peptide is 0.65 g / L. According to the self-researched anti-freezing agent disclosed by the invention, the low-temperature tolerance of grape leaves is remarkably improved by optimizing a formula. Moreover, the low-temperature tolerance duration is obviously prolonged, the composition takes effect 24 hours after being sprayed, the lasting period can reach 192 hours at most and is 120 hours far away from that of products sold in supermarkets, and particularly, the composition can still keep effective protection at the extremely low temperature (-6.5 DEG C). The method shows excellent effects in different grape varieties (such as Kyoho and black peak), and verification tests show that the method is high in adaptability and can cope with various low-temperature stress scenes.

Description

Technical Field

[0001] The invention belongs to the technical field of crop antifreeze, and particularly relates to an antifreeze agent for grapes and a preparation method thereof. Background Art

[0002] Grapes, a woody vine of the genus Vitis in the family Vitaceae, play a vital role in my country's agricultural production. In recent years, my country's major grape-producing regions have frequently experienced frost disasters, severely hampering the modernization of the grape industry. Frost disasters often occur during the bud and leaf expansion period, when cold air frequently moves through northern regions, causing near-ground temperatures to plummet to 0°C or below, causing a rapid drop in the surface temperature of grape buds and young leaves. This low temperature rapidly freezes and dehydrates young tissue cells, disrupting the plant's normal metabolism and causing damage to new tissues such as shoots, buds, and young leaves. In severe cases, it can even lead to plant death, resulting in significant yield reductions or even complete failure.

[0003] Despite the wide variety of antifreeze products on the market, these products generally suffer from unstable effectiveness and a lack of specificity. This is because different tree species have varying sensitivities to low temperatures, and general-purpose antifreeze products often fail to meet the needs of specific crops. Therefore, it is crucial to develop specialized antifreeze products tailored to specific fruit trees, combining precise combinations of plant growth regulators and nutrients. This can not only significantly improve a tree's frost resistance but also minimize negative impacts on plant growth and fruit quality. Summary of the Invention

[0004] In view of this, the object of the present invention is to provide an antifreeze agent for grapes and a preparation method thereof. The antifreeze agent prepared by the present invention can significantly improve the antifreeze ability of grape leaves and has the characteristics of fast effect and long lasting effect.

[0005] In order to achieve the above object, the present invention provides the following technical solutions:

[0006] The present invention provides an antifreeze agent for grapes, comprising the following components in the following mass ratios:

[0007] Brassinolide: sodium nitrophenolate: abscisic acid: small molecule peptide = 0.26:60:35:650.

[0008] Preferably, the concentrations of the components are 0.26 mg / L brassinolide, 60 mg / L sodium nitrophenolate, 35 mg / L abscisic acid, and 0.65 g / L small molecule peptide.

[0009] Preferably, the dosage of the antifreeze agent is 500 ml / tree for each spraying, and each 500 ml of leaf antifreeze agent, except for the various components, is supplemented with water.

[0010] Preferably, the antifreeze lasts for 192 hours.

[0011] As an advantage, the method comprises the following steps:

[0012] Weigh the raw materials according to the mass ratio;

[0013] The raw materials are dissolved in a solvent to prepare the antifreeze agent at the concentration.

[0014] Contains at least the following beneficial technical effects:

[0015] The self-developed antifreeze of the present invention significantly improves the low temperature tolerance of grape leaves by optimizing the formula. It also significantly improves the low temperature tolerance time, taking effect 24 hours after spraying, and lasting up to 192 hours (8 days), far exceeding the 120 hours (5 days) of commercially available products, especially at extremely low temperatures (-6.5°C). Through orthogonal experiments and TOPSIS analysis, ineffective ingredients (such as potassium dihydrogen phosphate and aminobutyric acid) were eliminated, and key ingredients (brassinolide, abscisic acid, and small molecule peptides) were optimized, solving the problem of unstable effects of general antifreezes and achieving precise protection of grape leaves. It has shown excellent effects in different grape varieties (such as Kyoho and Black Peak), and verification tests have shown that it has strong adaptability and can cope with a variety of low temperature stress scenarios. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is the preliminary antifreeze composition optimization of Example 2 to determine the freezing damage conditions of each treatment.

[0017] Figure 2 The antifreeze composition of Example 2 was further optimized to treat the freezing damage.

[0018] Figure 3 The frost damage conditions of each treatment in Example 3 are shown.

[0019] Figure 4 This is the freezing damage situation during the effective period of each treatment under low temperature in Example 3. DETAILED DESCRIPTION

[0020] Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative work shall fall within the scope of protection of the present invention. The present invention will be described below with reference to specific embodiments. It should be noted that these embodiments are merely illustrative and do not limit the present invention in any way.

[0021] Example 1

[0022] Research Methodology:

[0023] Five-year-old greenhouse grapes (Kyoho) were selected as test material. Ten trees of similar growth were selected for each treatment and marked to ensure consistency in experimental conditions. During the leaf expansion period (4-6 leaves), the grapes were sprayed with antifreeze foliarly between 8 and 9 a.m. Leaf collection began 48 hours after spraying and continued every 24 hours, with sampling occurring between 8 and 9 a.m. to prevent desiccation of the young leaves.

[0024] Before sampling, a high-precision, energy-saving thermostatic bath (GDH-4006) measuring 0.16 m x 0.18 m x 0.12 m was filled with alcohol. After starting the bath, the preset temperature was adjusted. For sampling, 10 functional leaves of similar size were selected for each treatment. The expanded leaves, along with their petioles, were collected, placed in a ziplock bag, and quickly placed in a thermostatic bath for low-temperature testing. After sampling, the bag containing the leaves was sealed with masking tape and quickly placed in a thermostatic bath that had reached the preset temperature.

[0025] The low-temperature test was conducted at the following temperatures: -1°C, -1.5°C, -2°C, -2.5°C, -3°C, -3.5°C, -4°C, -4.5°C, -5°C, and -5.5°C. Alcohol was poured into the thermostat and the instrument was activated. When the temperature reached the preset value, the sample was placed inside and maintained at the set temperature for four hours to simulate a low-temperature stress environment. After the low-temperature simulation, the sample was removed and allowed to recover at room temperature for six hours. Leaf browning was observed, and the frozen leaf area was calculated.

[0026] In order to obtain the concentration of the initial screening formula of antifreeze, a 4-factor 4-level orthogonal test L16 (4 4 ) design (Table 1), with a total of 16 treatments. After collecting leaves, a low-temperature stress test was conducted at the above-mentioned set temperature. The freezing results were counted 20 days after the initial screening.

[0027] Table 1 Antifreeze ratios for different treatments

[0028]

[0029]

[0030] Research Results:

[0031] Antifreeze ingredients and ratios were screened based on the unfrozen area of ​​grape leaves, with 50% of the unfrozen area used as the standard for screening. Table 2 shows that some test materials showed good frost resistance at temperatures between -2°C and -4.5°C, while all treatments suffered frost damage at temperatures as low as -5°C. Therefore, this test only included the unfrozen area between -2°C and -4.5°C. Treatment 11 had an unfrozen area of ​​99% and tolerated an extreme low temperature of -4.5°C, demonstrating the best frost resistance.

[0032] Table 2 Unfrozen area of ​​grape leaves in each treatment combination

[0033]

[0034] Note: '——' in the table means not measured (according to the previous temperature results, it shows that the treatment effect is not good)

[0035] At -4°C, the unfrozen leaf area varied significantly among the treatments. Therefore, the unfrozen grape leaf area obtained at -4°C was selected for analysis of the antifreeze's long-lasting effectiveness. The results are shown in Table 3. Treatment 11 was selected as the optimal formulation at this temperature, with a long-lasting effect of up to 120 hours after spraying.

[0036] Table 3 Comparison of the effectiveness of antifreeze under different treatments

[0037]

[0038] Based on the results of TOPSIS analysis, the concentration and composition of antifreeze were optimized (Table 4):

[0039] Table 4 Theoretical optimal concentration and influence ranking of each component

[0040]

[0041]

[0042] Brassinolide: ranked first in influence, with the optimal concentration being 0.5 mg / L (0 mg / L, 0.25 mg / L, 0.5 mg / L, 0.75 mg / L). The concentration gradient will be refined in the next optimization step.

[0043] Sodium nitrophenolate: ranked second in terms of influence, with the optimal concentration being 54 mg / L (0 mg / L, 18 mg / L, 36 mg / L, 54 mg / L). The concentration gradient will be refined in the next optimization step.

[0044] Potassium dihydrogen phosphate: The influence ranked third, and the optimal concentration was 0g / L (0g / L, 1.5g / L, 3g / L, 4.5g / L). This indicates that this component did not have a good effect on improving the frost resistance of the test material under the experimental setting concentration. This component will be eliminated in the next optimization step.

[0045] GABA: ranked fourth in influence, with the optimal concentration being 0 g / L (0 g / L, 0.25 g / L, 0.5 g / L, 0.75 g / L). However, since this component had the lowest influence, it indicated that this component was not effective in improving the frost resistance of the test materials in the experiment, and this component was eliminated in the next optimization step.

[0046] Example 2

[0047] Antifreeze ingredient optimization and comparison with similar products

[0048] Preliminary antifreeze composition optimization

[0049] Research Methodology:

[0050] Five-year-old greenhouse grapes (Kyoho) were selected as test material. Ten trees of similar growth were selected for each treatment and marked to ensure consistency in experimental conditions. During the leaf expansion period (4-6 leaves), the grapes were sprayed with antifreeze foliarly between 8 and 9 a.m. Leaf collection began 48 hours after spraying and continued every 24 hours, with sampling occurring between 8 and 9 a.m. to prevent desiccation of the young leaves.

[0051] Before sampling, a high-precision, energy-saving thermostatic bath (GDH-4006) measuring 0.16 m x 0.18 m x 0.12 m was filled with alcohol. After starting the bath, the preset temperature was adjusted. For sampling, 10 functional leaves of similar size were selected for each treatment. The expanded leaves, along with their petioles, were collected, placed in a ziplock bag, and quickly placed in a thermostatic bath for low-temperature testing. After sampling, the bag containing the leaves was sealed with masking tape and quickly placed in a thermostatic bath that had reached the preset temperature.

[0052] The low-temperature test was set at -2.5°C, -3°C, -3.5°C, -4°C, -4.5°C, and -5°C. Alcohol was poured into the thermostat and the instrument was started. When the temperature reached the preset value, the sample was placed inside and maintained at the set temperature for four hours to simulate a low-temperature stress environment. After the low-temperature simulation, the sample was removed and allowed to recover at room temperature for six hours. The leaf browning was observed, and the frozen leaf area was calculated.

[0053] In order to optimize the initial screening formula of antifreeze, a 5-factor 4-level orthogonal test L16 (4 5 ) design (Table 5). The five factors are brassinolide, sodium nitroprusside, glucose, abscisic acid, and small molecule peptides (small molecule peptides are short-chain amino acids composed of 2 to 10 amino acids connected by peptide bonds). The small molecule peptides in this experiment are short-chain amino acid products prepared by Shandong Changxiao Bioengineering Co., Ltd. using soybean protein as raw material and enzymatic hydrolysis and other technologies (the same below). At the same time, two antifreeze products with better antifreeze effects on the market, product 1 (Baoguo 303) and product 2 (Baoguoliang), were selected for comparative experiments, with a total of 18 treatments. After collecting the leaves, a low temperature stress test was carried out at the above-mentioned set temperature.

[0054] Table 5 Optimized formula of antifreeze for different treatments

[0055]

[0056]

[0057] Study Results

[0058] The optimization results of antifreeze are shown in Table 6. At the same time, the screening was carried out based on the standard of 50% unfrozen area. At -2.5℃ and -3℃, all test materials did not suffer from frost damage; at -3.5℃ to -5℃, some test materials had good antifreeze effect; at -5.5℃, all treatments suffered from frost damage ( Figure 1 Therefore, the test results only count the unfrozen area of ​​the test materials at -3.5℃~5℃.

[0059] Treatments 6 and 12 both had a minimum temperature tolerance of -5°C, with 97% and 96% of the unfrozen area, respectively. In contrast, the unfrozen areas of Products 1 and 2 at -3.5°C were 21% and 0%, respectively. This indicates that the optimized formula in this experiment exhibits significantly higher antifreeze efficacy than existing products 1 and 2.

[0060] Table 6 The leaves of each treatment were not frozen under different low temperature treatments

[0061]

[0062]

[0063] Note: The test materials did not suffer frost damage at -2.5℃ and -3℃, but suffered frost damage at -5.5℃. Therefore, the average value of the unfrozen area is the result calculated under the conditions of -3.5℃ to -5℃.

[0064] The optimal theoretical antifreeze formula obtained according to the SAS statistical analysis results is: brassinolide 0.3 mg / L, sodium nitrophenolate 36 mg / L, glucose 3 g / L, abscisic acid 20 mg / L, and small molecule peptide 1 g / L.

[0065] According to the results of TOPSIS analysis (Table 7), the concentration and composition of the antifreeze were optimized again:

[0066] Abscisic acid: ranked first in influence, with the optimal concentration being 20 mg / L (optimized concentrations are 0 mg / L, 10 mg / L, 15 mg / L, and 20 mg / L). The concentration gradient will be refined in the next optimization step.

[0067] Small molecule peptides: ranked second in influence, with the optimal concentration being 1 g / L (optimized concentrations are 0 g / L, 0.5 g / L, 1 g / L, and 1.5 g / L). The concentration gradient will be refined in the next optimization step to save costs.

[0068] Sodium pyrophosphate nitrate: ranked third in terms of influence, with the best concentration being 36 mg / L (optimized concentrations are 0 mg / L, 18 mg / L, 27 mg / L, and 36 mg / L). The next step of optimization will continue to increase the concentration.

[0069] Glucose: ranked fourth in influence, with the optimal concentration being 3 g / L (optimized concentrations are 0 g / L, 3 g / L, 5 g / L, and 7 g / L). The concentration gradient will be reduced in the next optimization step.

[0070] Brassinolide: ranked fifth in influence, with the optimal concentration being 0.3 mg / L (optimized concentrations are 0 mg / L, 0.2 mg / L, 0.3 mg / L, and 0.4 mg / L). The concentration gradient will be refined in the next step of optimization.

[0071] Table 7 Theoretical optimal concentration and influence ranking of each component

[0072]

[0073] Treatment 6 (0.2 mg / L brassinolide, 18 mg / L sodium nitrophenolate, 20 mg / L abscisic acid, 1 g / L small molecule peptide), treatment 12 (0.3 mg / L brassinolide, 36 mg / L sodium nitrophenolate, 3 g / L glucose, 1 g / L small molecule peptide) and the optimal theoretical antifreeze formula (0.3 mg / L brassinolide, 36 mg / L sodium nitrophenolate, 3 g / L glucose, 20 mg / L abscisic acid, 1 g / L small molecule peptide).

[0074] Further antifreeze optimization test

[0075] Study plan

[0076] From May 22, 2023 to June 20, 2023, an antifreeze optimization test was carried out, and integrated water and fertilizer management technology was adopted.

[0077] Research Methodology:

[0078] Five-year-old greenhouse grapes (Kyoho) were selected as test material. Ten trees of similar growth were selected for each treatment and marked to ensure consistency in experimental conditions. During the leaf expansion period (4-6 leaves), the grapes were sprayed with antifreeze foliarly between 8 and 9 a.m. Leaf collection began 48 hours after spraying and continued every 24 hours, with sampling occurring between 8 and 9 a.m. to prevent desiccation of the young leaves.

[0079] Before sampling, a high-precision, energy-saving thermostatic bath (GDH-4006) measuring 0.16 m x 0.18 m x 0.12 m was filled with alcohol. After starting the bath, the preset temperature was adjusted. For sampling, 10 functional leaves of similar size were selected for each treatment. The expanded leaves, along with their petioles, were collected, placed in a ziplock bag, and quickly placed in a thermostatic bath for low-temperature testing. After sampling, the bag containing the leaves was sealed with masking tape and quickly placed in a thermostatic bath that had reached the preset temperature.

[0080] The low-temperature test was conducted at -4°C, -4.5°C, -5°C, -5.5°C, -6°C, and -6.5°C. Alcohol was poured into the thermostat and the instrument was activated. When the temperature reached the preset value, the samples were placed inside and maintained at the set temperature for four hours to simulate a low-temperature stress environment. After the low-temperature simulation, the samples were removed and allowed to recover at room temperature for six hours. The leaves were observed for browning and the frozen area was calculated.

[0081] In order to further optimize the antifreeze formulation, a 5-factor 4-level orthogonal experiment L16 (4 5 ) design (Table 8). Furthermore, two commercially available antifreeze agents, Product 1 (Baoguo 303) and Product 2 (Baoguoliang), with excellent antifreeze effects, were selected for comparative testing, resulting in a total of 18 treatments. After collecting leaves, a low-temperature stress test was conducted at the aforementioned set temperature.

[0082] Table 8 Optimized formula of antifreeze for different treatments

[0083]

[0084]

[0085] Research Results:

[0086] On the basis of multiple previous tests, the temperature was directly set at or below the semi-lethal tolerance temperature for the optimization test of each antifreeze treatment. The test results (Table 9) showed that the grape leaves of treatment 6, treatment 11 and treatment 13 performed well at a low temperature of -6°C, with the lowest tolerance temperature of -6°C and the unfrozen areas of the leaves being 100%, 100% and 78% respectively; while the lowest tolerance temperatures of product 1 and product 2 were -4.5°C and -4°C respectively, with the unfrozen areas being 30% (-4.5°C) and 41% (-4°C). The results showed that the antifreeze effect of the optimized antifreeze formula was significantly better than that of product 1 and product 2 ( Figure 2 ).

[0087] Table 9 The leaves of each treatment were not frozen under different low temperature treatments

[0088]

[0089]

[0090] The optimal theoretical formula obtained based on the SAS analysis results is (Table 10): brassinolide 0.275 mg / L, sodium nitrophenolate 60 mg / L, glucose 0.5 g / L, abscisic acid 25 mg / L, and small molecule peptide 0.7 g / L.

[0091] Table 10 Effects of different antifreeze compounds on low temperature resistance of grape leaves

[0092]

[0093] Example 3

[0094] Self-developed antifreeze verification examples and validity period investigation

[0095] Research Methodology:

[0096] Five-year-old greenhouse grapes (Black Peak) were selected as test material. Ten trees of similar growth were selected for each treatment and marked to ensure consistency in experimental conditions. During the leaf expansion period (4-6 leaves), the grapes were sprayed with antifreeze foliarly between 8 and 9 a.m. Leaf collection began 48 hours after spraying and continued every 24 hours, with sampling occurring between 8 and 9 a.m. to prevent desiccation of the young leaves.

[0097] Before sampling, a high-precision, energy-saving thermostatic bath (GDH-4006) measuring 0.16 m x 0.18 m x 0.12 m was filled with alcohol. After starting the bath, the preset temperature was adjusted. For sampling, 10 functional leaves of similar size were selected for each treatment. The expanded leaves, along with their petioles, were collected, placed in a ziplock bag, and quickly placed in a thermostatic bath for low-temperature testing. After sampling, the bag containing the leaves was sealed with masking tape and quickly placed in a thermostatic bath that had reached the preset temperature.

[0098] The low-temperature test was conducted at -4°C, -4.5°C, -5°C, -5.5°C, -6°C, -6.5°C, -7°C, and -7.5°C. Alcohol was poured into the thermostat and the instrument was activated. When the temperature reached the preset value, the sample was placed inside and maintained at the set temperature for four hours to simulate a low-temperature stress environment. After the low-temperature simulation, the sample was removed and allowed to recover at room temperature for six hours. Leaf browning was observed, and the frozen leaf area was calculated.

[0099] In order to verify the optimized antifreeze formula, the experiment was designed with 6 treatments (Table 11). Treatments 1 to 4 were treatments 1, 6, 11, and 13 in the optimization experiment 2.3, respectively. Treatment 5 was product 1 (good fruit bracts), and treatment 6 was product 2 (303 fruit bracts).

[0100] In addition, in order to conduct a persistence investigation, the optimized formula and the product were subjected to a persistence comparison test at the same time. The temperature was directly set at the semi-lethal tolerance temperature and below (-4.5℃, -5.5℃, -6.5℃), and the persistence period was 24h, 48h, 72h, 96h, 120h, 144h, 168h and 192h after the antifreeze was sprayed.

[0101] Table 11 Verification formula of antifreeze with different treatments

[0102]

[0103]

[0104] Note: T1, T2, T3, and T4 are treatment 1, treatment 6, treatment 11, and treatment 13 in optimization experiment 2.3, respectively.

[0105] Study Results

[0106] The test results are shown in Table 12. Treatment 2 has the best antifreeze effect, with the lowest tolerable temperature of -7°C and 100% of the leaves unfrozen; Treatment 3 has the lowest tolerable temperature of -6°C and 95% of the leaves unfrozen; Treatment 4 has the lowest tolerable temperature of -6.5°C and 55% of the leaves unfrozen; T5 has the lowest tolerable temperature of -4.5°C and T6 has the lowest tolerable temperature of -4°C. Figure 3 The results of this verification test show that spraying the self-developed antifreeze can effectively reduce the occurrence of frost damage to grape leaves.

[0107] Table 12 The leaves of each treatment were not frozen under different low temperature treatments

[0108]

[0109] The results of the investigation on the effective period of antifreeze showed (Table 13) that the effective period of treatment 2, treatment 3, and treatment 4 was 24 hours after spraying. The effective period of treatment 2 at -6.5℃ was 192 hours after spraying; the effective period of treatment 3 and treatment 4 at -5.5℃ was 120 hours after spraying; the effective period of treatment 5 (product 1) at -4.5℃ was 120 hours after spraying; all the plants in treatment 6 (product 2) suffered frost damage at -4.5℃. Figure 4 ).

[0110] Table 13 Investigation on the effectiveness of different compound antifreezes and existing products

[0111]

[0112]

[0113] in conclusion

[0114] This study tested seven ingredients in combination: brassinolide, abscisic acid, sodium nitrophenolate, aminobutyric acid, small-molecule peptides, glucose, and potassium dihydrogen phosphate. Baoguoliang and Baoguo 303 (Baoguoyou) were selected as the market's preferred antifreeze products. (Baoguoliang and Baoguo 303 (Baoguoyou), fertilizers developed by Taiwan's Jiuan Company, demonstrated excellent low-temperature resistance during the late frost in Fujian Province in the spring of 2008. Research by Lou Yusui et al. also showed that Baoguo 303 (Baoguoyou) and Baoguoliang, along with four other antifreeze agents, significantly improved the frost resistance of young grape buds.)

[0115] Different grape varieties have different minimum tolerance temperatures for grape leaves treated with the self-developed antifreeze. Taking Kyoho grape leaves as antifreeze test materials, the minimum tolerance temperature of self-developed antifreeze treatment 6 (0.26 mg / L brassinolide, 60 mg / L sodium nitrophenolate, 35 mg / L abscisic acid, 0.65 g / L small molecule peptide) is -6°C, while the minimum tolerance temperatures of Baoguoliang and Baoguo 303 are -4.5°C and -4°C respectively; taking Heifeng grape leaves as antifreeze test materials, the minimum tolerance temperature of self-developed antifreeze treatment 2 (0.26 mg / L brassinolide, 60 mg / L sodium nitrophenolate, 35 mg / L abscisic acid, 0.65 g / L small molecule peptide) is -7°C, while the minimum tolerance temperatures of Baoguoliang and Baoguo 303 are -4.5°C and -4°C respectively. It can be seen that the antifreeze effect of the self-developed antifreeze is significantly better than the existing products 1 and 2 on the market.

[0116] After years of testing, screening and verification, the best antifreeze formula was obtained: brassinolide 0.26 mg / L, sodium nitrophenolate 60 mg / L, abscisic acid 35 mg / L, and small molecule peptide 0.65 g / L.

[0117] Grape Late Frost Antifreeze significantly improves the frost resistance of grape leaves with rapid, long-lasting effects. Application should begin 24 hours before frost (based on weather forecasts and warnings), and begins taking effect at the onset of frost (24 hours after spraying). The effect lasts up to 192 hours.

[0118] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. An antifreeze agent for grapes, characterized in that: The composition comprises the following components in the following mass ratios: Brassinolide: sodium nitrophenolate: abscisic acid: small molecule peptide = 0.26:60:35:

650.

2. The antifreeze agent according to claim 1, characterized in that The concentrations of the components are 0.26 mg / L of brassinolide, 60 mg / L of sodium nitrophenolate, 35 mg / L of abscisic acid, and 0.65 g / L of small molecule peptide.

3. The antifreeze according to claim 2, characterized in that The dosage of the antifreeze agent is 500 ml per tree for each spraying. In addition to the various components, the rest of each 500 ml leaf antifreeze agent is supplemented with water.

4. The antifreeze according to claim 2, characterized in that The duration of action of the antifreeze is 192 hours.

5. The method for preparing the antifreeze according to claim 2, characterized in that: The following steps are involved: Weigh the raw materials according to the mass ratio; The raw materials are dissolved in a solvent to prepare the antifreeze agent at the concentration.

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