Slide loading device and immunohistochemical analyzer

Abstract

The invention provides a slide loading device and an immunohistochemical analyzer. The slide loading device comprises a base, a first supporting table, a second supporting table, a slice supporting piece and a driving mechanism. The first supporting table is arranged on the base, and the second supporting table is slidably arranged on the first supporting table; the section supporting piece is connected with the first supporting plate, located above the second supporting table and used for supporting the end of the tissue section. The slide is placed on the second supporting table, one end of the tissue slice is placed on the slide, the other end of the tissue slice is placed on the slice supporting piece, then warm water is injected into the end, making contact with the slide, of the tissue slice, and the second supporting table slides relative to the first supporting table under driving of the driving mechanism; therefore, the slice supporting piece is gradually far away from the tissue slice, and the space between the tissue slice and the slide is filled with warm water under the adhesion of the warm water, so that the gas between the tissue slice and the slide is reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of experimental equipment, in particular to a slide loading device and an immunohistochemical analyzer. Background Art

[0002] Immunohistochemistry uses the principle of specific binding between antigens and antibodies to determine the antigens (peptides and proteins) in tissue cells by chemically reacting the colorants (fluorescein, enzymes, metal ions, isotopes) labeled with antibodies to detect their color and to locate and characterize them.

[0003] When conducting immunohistochemistry experiments, tissue sections need to be placed on anti-shedding glass slides. Due to the presence of bubbles between the tissue sections and the anti-shedding glass slides, the two will not fit completely together. After staining is completed and the slides are finally sealed, when observing and photographing under a microscope, it will be found that due to the presence of bubbles during the experiment, the local staining effect of the tissue sections is poor, affecting the observation. Summary of the Invention

[0004] In view of the deficiencies in the prior art, the present invention provides a slide loading device and an immunohistochemical analyzer, thereby solving or at least alleviating one or more of the above-mentioned problems and other problems in the prior art.

[0005] The present invention provides a slide loading device, comprising:

[0006] base;

[0007] A first support platform is provided on the base;

[0008] a second supporting platform, slidably disposed on the first supporting platform, for loading a slide;

[0009] a slice support connected to the first support plate, the slice support located above the second support platform, and used to support the end of the tissue slice; and

[0010] A driving mechanism is used to drive the second supporting platform to slide on the first supporting platform.

[0011] Preferably, it also includes:

[0012] a water tank, disposed on the base; and

[0013] a stopper connected to the second support platform and used to block the tissue slice on the second support platform;

[0014] The first support platform is rotatably connected to the base; one end of the first support platform can be rotated into the water tank; and the driving mechanism is further used to drive the first support platform to rotate.

[0015] Preferably, the first support platform comprises:

[0016] a first support plate; and

[0017] at least one pair of limit blocks, wherein the lower ends of each pair of limit blocks are respectively connected to two sides of the first support plate;

[0018] Both sides of the second support platform are respectively slidably matched with the corresponding limit blocks; the driving mechanism includes a rocker arm; one end of the rocker arm is connected to the end of the first support plate, and the middle part of the rocker arm is rotatably connected to the base.

[0019] Preferably, a mounting window is provided on the first supporting plate;

[0020] The driving mechanism further comprises:

[0021] An arc-shaped guide block is connected to the base, and a plurality of evenly distributed tooth grooves are formed on the outer wall of the arc-shaped guide block;

[0022] a driving gear rotatably disposed within the mounting window; and

[0023] a driving rack, arranged at the bottom of the second supporting platform along the sliding direction of the second supporting platform;

[0024] The driving gear is engaged with the driving rack; the driving gear can be engaged with the tooth groove.

[0025] Preferably, a limiting groove is formed by removing a tooth from the driving gear;

[0026] The driving mechanism further comprises:

[0027] a connecting piece connected to one end of the driving ring;

[0028] a first mating tooth, the first mating tooth being connected to the connecting member, the first mating tooth being located on a side of the limiting groove, and the first mating tooth being located on a side of the arc-shaped guide block; and

[0029] a second mating tooth, the second mating tooth being connected to a side surface of the arc-shaped guide block, and the second mating tooth being capable of abutting against the first mating tooth;

[0030] Both sides of the mouth of the limiting groove can abut against the outer wall of the arc-shaped guide block. When the first matching tooth abuts against the second matching tooth, the driving gear can rotate and engage with the tooth groove.

[0031] Preferably, two ends of the stopper are engaged with two sides of the second support platform.

[0032] Preferably, the tissue support member is a plurality of parallel support rods; both ends of the support rods are respectively connected to the corresponding limit blocks.

[0033] The present invention also provides an immunohistochemical analyzer comprising any one of the above-mentioned slide loading devices.

[0034] Compared with the prior art, the present invention has the following beneficial effects:

[0035] In the technology of the present invention, a glass slide is placed on a second support table, one end of the tissue slice is placed on the glass slide, and the other end of the tissue slice is placed on a slice support, and then warm water is injected into the contact end of the tissue slice and the glass slide. Under the drive of the driving mechanism, the second support table slides relative to the first support table, so that the slice support gradually moves away from the tissue slice, and the entire tissue slice gradually adheres to the glass slide from the end. Under the adhesion of warm water, the space between the tissue slice and the glass slide is filled with warm water, thereby reducing the gas between the tissue slice and the glass slide, so that the tissue slice and the glass slide are closely adhered, the staining effect is improved, and better surface quality of the slice is obtained. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following briefly describes the drawings required for the specific embodiments or the description of the prior art. Similar elements or parts are generally identified by similar reference numerals throughout the drawings. Elements or parts in the drawings are not necessarily drawn to scale.

[0037] Figure 1 is a three-dimensional diagram of a slide loading device according to an embodiment of the present invention;

[0038] Figure 2 for Figure 1 Another stereogram of

[0039] Figure 3 for Figure 1 Another stereogram of (without sink);

[0040] Figure 4 for Figure 1 A three-dimensional diagram of the middle drive mechanism cooperating with the first support platform;

[0041] Figure 5 for Figure 4 Schematic diagram of the coordination between the middle arc guide block, the drive gear and the drive rack;

[0042] Figure 6 for Figure 4 A three-dimensional diagram of the cooperation between the middle drive gear and the arc-shaped guide block;

[0043] Figure 7 for Figure 6 Another stereogram of

[0044] Figure 8 for Figure 6 A three-dimensional view of the middle drive gear.

[0045] Reference numerals:

[0046] 10. Base;

[0047] 20. First support platform; 21. First support plate; 211. Mounting window; 22. Limit block;

[0048] 30. Second support platform;

[0049] 40. Glass slide;

[0050] 50. Slice support;

[0051] 60. Tissue sections;

[0052] 70. Driving mechanism; 71. Rocker arm; 72. Arc-shaped guide block; 721. Tooth groove; 73. Driving gear; 74. Driving rack; 741. Limiting groove; 75. Connecting member; 76. First mating tooth; 77. Second mating tooth;

[0053] 80. Sink; 81. Block. DETAILED DESCRIPTION

[0054] The following embodiments of the technical solution of the present invention will be described in detail with reference to the accompanying drawings. The following embodiments are only used to more clearly illustrate the technical solution of the present invention and are therefore only examples and are not intended to limit the scope of protection of the present invention.

[0055] It should be noted that, unless otherwise specified, the technical or scientific terms used in this application should have the common meanings understood by those skilled in the art to which the present invention belongs.

[0056] In the description of this application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like to indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0057] In addition, the terms "first," "second," etc. are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the technical features indicated. In the description of the present invention, "plurality" means more than two, unless otherwise specifically defined.

[0058] In this application, unless otherwise expressly specified or limited, terms such as "mounted," "connected," "connect," and "fixed" should be understood in a broad sense. For example, they may refer to fixed or detachable connections, or integration; mechanical or electrical connections; direct or indirect connections through an intermediate medium; and internal communication between two components or interaction between two components. Those skilled in the art will understand the specific meanings of the above terms in this application based on specific circumstances.

[0059] In this application, unless otherwise expressly specified or limited, when a first feature is "above" or "below" a second feature, it may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Furthermore, when a first feature is "above," "above," or "above" a second feature, it may mean that the first feature is directly above or diagonally above the second feature, or simply means that the first feature is at a higher level than the second feature. When a first feature is "below," "below," or "below" a second feature, it may mean that the first feature is directly below or diagonally below the second feature, or simply means that the first feature is at a lower level than the second feature.

[0060] See also Figures 1 to 8 This embodiment provides a slide loading device, including a base 10, a first support platform 20, a second support platform 30, a slice support 50 and a driving mechanism 70.

[0061] The first support platform 20 is mounted on the base 10. The second support platform 30 is slidably mounted on the first support platform 20 and is used to load a glass slide 40. A slice support 50 is connected to the first support plate 21 and is positioned above the second support platform 30. The slice support 50 is used to support the end of a tissue slice 60. The second support platform 30 is positioned between the slice support 50 and the first support platform 20. A drive mechanism 70 is used to drive the second support platform 30 to slide on the first support platform 20.

[0062] In conventional immunohistochemistry experiments, the tissue slice 60 is directly and entirely covered on the glass slide 40 , so that the gas at the lower end of the tissue slice 60 is enclosed in the space between the tissue slice 60 and the glass slide 40 .

[0063] In this embodiment, the glass slide 40 is placed on the second support platform 30, one end of the tissue slice 60 is placed on the glass slide 40, and the other end of the tissue slice 60 is placed on the slice support 50, and then warm water is injected into the contact end of the tissue slice 60 and the glass slide 40, so that the tissue slice 60 and the glass slide 40 are adhered to each other; then, under the drive of the driving mechanism 70, the second support platform 30 slides relative to the first support platform 20, so that the slice support 50 gradually moves away from the tissue slice 60, and the tissue slice 60 loses the support of the slice support 50, and the entire tissue slice 60 gradually adheres to the glass slide 40 from the end. Under the adhesion of warm water, the space between the tissue slice 60 and the glass slide 40 is filled with warm water, so that the gas between the tissue slice 60 and the glass slide 40 is reduced, so that the tissue slice 60 and the glass slide 40 are closely adhered, the staining effect is improved, and a better appearance quality of the slice is obtained.

[0064] In one embodiment, the slide 40 loading device further includes a water tank 80 and a stopper 81 .

[0065] The water tank 80 is provided on the base 10. The first support platform 20 is rotatably connected to the base 10, and one end of the first support platform 20 can be rotated into the water tank 80. The stopper 81 is connected to the second support platform 30 and is used to block the tissue slice 60 on the second support platform 30. The stopper 81 and the slice support 50 are both located above the second support platform 30. Specifically, when the second support platform 30 rotates following the first support platform 20, the tissue slice 60 above the glass slide 40 placed on the second support platform 30 is in an inclined state. The stopper 81 is located at the end of the tissue slice 60, and the stopper 81 forms a block for the tissue slice 60 to prevent the tissue slice 60 from sliding on the glass slide 40. The driving mechanism 70 is also used to drive the first support platform 20 to rotate.

[0066] In this embodiment, Figure 2The slide 40 is loaded onto the second support table 30. The left end of the tissue section 60 is bonded to the slide 40 and abuts against the stopper 81. The right end of the tissue section 60 is placed on the section support 50. The drive mechanism 70 drives the first support table 20 to rotate counterclockwise, so that the left end of the tissue section 60 is first immersed in the warm water in the water tank 80. At the same time, the second support table 30 slides leftward relative to the first support table 20, so that the tissue section 60 is gradually separated from the section support 50. Then, the tissue section 60 is gradually bonded to the slide 40 from its left end to its right end. On the one hand, the tissue slice 60 gradually adheres to the glass slide 40, thereby preventing the gas from being trapped in the space between the tissue slice 60 and the glass slide 40. On the other hand, the warm water gradually penetrates from the left end of the tissue slice 60 to the right, thereby expelling the air between the tissue slice 60 and the glass slide 40 to a certain extent. Moreover, the rotating first support table 20 makes the space between the tissue slice 60 and the glass slide 40 on the second support table 30 open upward. When the tissue slice 60 is immersed in the warm water, the gas between the tissue slice 60 and the glass slide 40 is discharged due to the upward buoyancy of the water, thereby further making the tissue slice 60 and the glass slide 40 adhere tightly.

[0067] In one embodiment, the first support platform 20 includes a first support plate 21 and at least one pair of limiting blocks 22 .

[0068] The lower ends of each pair of limit blocks 22 are connected to the sides of the first support plate 21. The sides of the second support platform 30 are slidably engaged with the corresponding limit blocks 22. Specifically, the upper inner ends of the limit blocks 22 are convex, while the sides of the second support platform 30 are concave. The limit blocks 22 slide in engagement with the side surfaces of the second support platform 30 via their convex portions. The drive mechanism 70 includes a rocker arm 71; one end of the rocker arm 71 is connected to the end of the first support plate 21, and the middle portion of the rocker arm is rotatably connected to the base 10.

[0069] In this embodiment, the first support plate 21 and the second support platform 30 are driven to rotate into or out of the water tank 80 by the rotation of the rocker arm 71 .

[0070] In one embodiment, a mounting window 211 is defined on the first support plate 21 .

[0071] The driving mechanism 70 further includes an arc-shaped guide block 72 , a driving gear 73 and a driving rack 74 .

[0072] The arc-shaped guide block 72 is connected to the base 10, and a plurality of evenly distributed tooth grooves 721 are provided on the outer wall of the arc-shaped guide block 72. The driving gear 73 is rotatably arranged in the installation window 211. The driving rack 74 is arranged at the bottom of the second support platform 30 along the sliding direction of the second support platform 30. The driving gear 73 is engaged with the driving rack 74, and the driving gear 73 can be engaged with the tooth groove 721. Specifically, the moving trajectory of the driving gear 73 is arc-shaped, and the center of the arc-shaped trajectory is located at the rotation center of the rocker arm 71. In addition, the center of the outer wall of the arc-shaped guide block 72 is also located at the rotation center of the rocker arm 71. The lower end of the rocker arm 71 can be abutted against the base 10, so that the rocker arm 71 can be rotated to a vertical state at most, at which time the first support platform 20 is horizontal.

[0073] In this embodiment, Figure 4 When the rocker arm 71 rotates and drives the first support plate 21 to rotate, the driving gear 73 in the installation window 211 engages with the tooth groove 721 on the outer wall of the arc-shaped guide block 72, thereby causing the driving gear 73 to rotate counterclockwise, and then the driving rack 74 is driven to move by the driving gear 73, so that the second support platform 30 slides on the first support plate 21.

[0074] In one embodiment, a limiting groove 741 is formed by removing a gear tooth from the driving gear 73 .

[0075] The driving mechanism 70 further includes a connecting member 75 , a first mating tooth 76 , and a second mating tooth 77 .

[0076] The connecting member 75 is connected to one end of the drive ring. The first mating tooth 76 is connected to the connecting member 75, and the first mating tooth 76 is located on the side of the limiting groove 741. The first mating tooth 76 is located on the side of the arc-shaped guide block 72. The second mating tooth 77 is connected to the side of the arc-shaped guide block 72, and the second mating tooth 77 can be abutted against the first mating tooth 76. The two sides of the mouth of the limiting groove 741 can be abutted against the outer wall of the arc-shaped guide block 72. When the first mating tooth 76 and the second mating tooth 77 are abutted, the driving gear 73 can be rotated and meshed with the tooth groove 721. For details, refer to Figure 4-Figure 8When the first support plate 21 is horizontal, the two sides of the mouth of the limiting groove 741 respectively abut against the curved outer wall of the arc-shaped guide block 72. At this time, the drive gear 73 cannot rotate. As the rocker arm 71 rotates counterclockwise by a certain angle, the first support plate 21 drives the end of the second support platform 30 to rotate into the water tank 80, and the warm water soaks a corner of the tissue slice 60. At this time, the first mating tooth 76 abuts the second mating tooth 77. As the rocker arm 71 rotates further, the drive gear 73 rotates, and the gear teeth of the drive gear 73 engage with the tooth grooves 721 on the outer wall of the arc-shaped guide block 72, allowing the drive gear 73 to drive the drive rack 74 to move, achieving the sliding of the second support platform 30 relative to the first support plate 21. In addition, when viewed from the side, the first mating tooth 76 is exactly located at the position of the missing gear tooth on the drive gear 73.

[0077] In one embodiment, both ends of the stopper 81 engage with both sides of the second support platform 30. Specifically, the stopper 81 is in an inverted U-shape, with both sides of the second support platform 30 being concave. The stopper 81 has a certain elasticity, so that the two arms of the stopper 81 engage with the concave sides of the second support platform 30. The stopper 81 can move and engage on the second support platform 30, thereby allowing the stopper 81 to block tissue slices 60 of different sizes, so that tissue slices 60 of different sizes can be properly supported by the slice support 50.

[0078] In one embodiment, the tissue support member is a plurality of parallel support rods; both ends of the support rods are respectively connected to corresponding limit blocks 22.

[0079] In this embodiment, when part of the tissue slice 60 is located on the support rod, it is in line contact with the support rod, so that while the support rod supports the tissue slice 60, the friction between the support rod and the tissue slice 60 is small, avoiding damage to the tissue slice 60 due to excessive friction when the tissue slice 60 and the support rod move relative to each other.

[0080] This embodiment provides an immunohistochemical analyzer, comprising any one of the above-mentioned slide loading devices.

[0081] In the description of the present invention, a large number of specific details are described. However, it is understood that embodiments of the present invention can be practiced without these specific details. In some instances, well-known methods, structures, and techniques are not shown in detail so as not to obscure the understanding of this description.

[0082] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the above embodiments, or make equivalent replacements for some or all of the technical features therein. These modifications or replacements do not deviate the essence of the corresponding technical solutions from the scope of the technical solutions of the embodiments of the present invention, and they should all be included in the scope of the claims and description of the present invention.

Claims

1. A slide loading device, characterized in that: include: Base (10); A first support platform (20) is provided on the base (10); a second supporting platform (30) slidably disposed on the first supporting platform (20) and used for loading a glass slide (40); a slice support (50) connected to the first support plate (21), the slice support (50) being located above the second support platform (30), and the slice support (50) being used to support the end of the tissue slice (60); as well as A driving mechanism (70) is used to drive the second supporting platform (30) to slide on the first supporting platform (20).

2. A slide loading device according to claim 1, characterized in that: Also includes: A water tank (80) is provided on the base (10); as well as a stopper (81), connected to the second support platform (30), and used to block the tissue slice (60) on the second support platform (30); The first support platform (20) is rotatably connected to the base (10); one end of the first support platform (20) can be rotated into the water tank (80); and the driving mechanism (70) is also used to drive the first support platform (20) to rotate.

3. A slide loading device according to claim 2, characterized in that: The first supporting platform (20) comprises: a first support plate (21); and at least one pair of limit blocks (22), wherein the lower ends of each pair of limit blocks (22) are respectively connected to two sides of the first support plate (21); Both sides of the second support platform (30) are respectively slidably engaged with the corresponding limit blocks (22); the driving mechanism (70) comprises a rocker arm (71); one end of the rocker arm (71) is connected to the end of the first support plate (21), and the middle part of the rocker arm is rotatably connected to the base (10).

4. A slide loading device as claimed in claim 3, characterized in that: The first support plate (21) is provided with an installation window (211); The driving mechanism (70) further comprises: An arc-shaped guide block (72) is connected to the base (10), and a plurality of evenly distributed tooth grooves (721) are formed on the outer wall of the arc-shaped guide block (72); a driving gear (73) rotatably disposed within the mounting window (211); and a driving rack (74) arranged at the bottom of the second support platform (30) along the sliding direction of the second support platform (30); The driving gear (73) is engaged with the driving rack (74); the driving gear (73) can be engaged with the tooth groove (721).

5. A slide loading device as claimed in claim 4, characterized in that: A limiting groove (741) is formed by removing a gear tooth from the driving gear (73); The driving mechanism (70) further comprises: a connecting member (75) connected to one end of the driving ring; a first mating tooth (76), the first mating tooth (76) being connected to the connecting member (75), the first mating tooth (76) being located on a side of the limiting groove (741), and the first mating tooth (76) being located on a side of the arc-shaped guide block (72); and a second mating tooth (77), the second mating tooth (77) being connected to a side surface of the arc-shaped guide block (72), and the second mating tooth (77) being capable of abutting against the first mating tooth (76); Both sides of the mouth of the limiting groove (741) can abut against the outer wall of the arc-shaped guide block (72), and when the first matching tooth (76) and the second matching tooth (77) abut against each other, the driving gear (73) can rotate and engage with the tooth groove (721).

6. A slide loading device according to claim 5, characterized in that: The two ends of the stopper (81) are clamped with the two sides of the second support platform (30).

7. A slide loading device according to claim 6, characterized in that: The tissue support member is a plurality of parallel support rods; both ends of the support rods are respectively connected to the corresponding limit blocks (22).

8. An immunohistochemical analyzer, characterized in that The device comprises a slide loading device as described in any one of claims 1 to 7.

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