A high-efficiency moisturizing repair composition and a preparation method thereof

Abstract

The application belongs to the technical field of cosmetics, and particularly relates to a high-efficiency moisturizing and repairing composition and a preparation method thereof. The high-efficiency moisturizing and repairing composition provided by the application is composed of artemisia capillaris extract, L-rhamnose, soluble protein polysaccharide and succinoglucan, has good moisturizing and repairing effects and is mild and non-irritating, and can be used for preparing high-efficiency moisturizing and repairing products. The preparation method of the artemisia capillaris extract adopts a freezing, ultrahigh-pressure treatment and flash extraction mode, has the advantages of improving extraction efficiency and avoiding degradation of active ingredients, is easy to operate, has low cost, can maximize the retention of effective components, and has a wide application prospect. The preparation method of the high-efficiency moisturizing and repairing composition is simple and easy to operate, is beneficial to industrialized production and application, and has a wide application prospect.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and particularly relates to a high-efficiency moisturizing and repairing composition and a preparation method thereof. BACKGROUND

[0002] In modern society, people generally face great work pressure or study pressure. When people are in a state of great pressure for a long time, and the climate environment changes and stimulates, the sebaceous glands and sweat glands of the skin secrete abnormally, the skin of people gradually ages, the sebum secretion decreases, and the ability of the skin to retain water decreases, so that the skin becomes dry, the stratum corneum of the skin epidermis thickens, and problems such as skin keratinization, dryness, and rough skin surface occur. In recent years, people's beauty consciousness has gradually increased, and therefore, how to moisturize and repair has become one of the research hotspots in the current skin care field.

[0003] At present, existing cosmetic compositions mainly add moisturizing agents such as hyaluronic acid, small-molecule polyols, polysaccharides and the like to increase the water content in the skin to achieve the effect of soothing and repairing the skin, or add ceramides to supplement the ceramides of the skin to achieve the effect of repairing the skin barrier. Although the above-mentioned existing methods have certain soothing and repairing effects on the skin, they also have some problems. For example, the moisturizing agent can only provide short-term water replenishment, and the skin repair effect is very limited; although the ceramides can repair the skin barrier, they cannot effectively soothe the allergic reaction of the skin in time; some skin care products contain a large amount of ethanol and other substances that are irritating to the skin, which can easily cause skin allergies in actual use; and some compositions have poor stability and can only achieve epidermal repair.

[0004] In summary, in order to better achieve the moisturizing and repairing effects, it is necessary to research a composition that has good moisturizing and repairing effects and is mild and non-irritating. SUMMARY

[0005] In view of the above defects or improvement needs of the prior art, the present application provides a high-efficiency moisturizing and repairing composition and a preparation method thereof, which solves the problems of limited effect and strong irritation of the existing moisturizing and repairing composition.

[0006] In order to achieve the above-mentioned purpose, the present application adopts the following technical scheme:

[0007] In a first aspect, the present application provides a high-efficiency moisturizing and repairing composition, which comprises the following components by weight: artemisia capillaris extract 14-18 parts, L-rhamnose 7-11 parts, soluble protein polysaccharide 9-13 parts, and succinoglycan 5-9 parts.

[0008] The preparation method of the artemisia capillaris extract comprises the following steps:

[0009] A, dry Artemisia capillaris, crush, pass through 30 mesh screen, to obtain Artemisia capillaris powder;

[0010] B, to the Artemisia capillaris powder, add ethanol solution, mix evenly, after freezing treatment, then perform ultra-high pressure treatment, to obtain a mixture;

[0011] C, the mixture is placed in a flash extractor for flash extraction, to obtain an extract;

[0012] D, the extract is centrifuged, the supernatant is rotary evaporated to alcohol-free, to obtain the Artemisia capillaris extract.

[0013] The Artemisia capillaris extract is rich in flavonoids, phenols, organic acids, volatile oils, terpenes and other active ingredients, and has multiple physiological activities such as liver protection, choleretic, anti-inflammatory, antioxidant, antiviral, antitumor, and lipid metabolism regulation.

[0014] L-rhamnose is a naturally occurring deoxy sugar, which has the properties of enhancing skin barrier, anti-inflammatory soothing, antioxidant, etc., and can also improve the permeability of other active ingredients.

[0015] Soluble protein polysaccharide is a protein polysaccharide solution extracted from salmon nasal cartilage tissue, which has one or more covalent bonds that can be connected to mucopolysaccharide substances, and has long-lasting moisturizing effect.

[0016] Succinoglycan is a kind of exopolysaccharide secreted by nitrogen-fixing microorganisms such as rhizobium, which has moisturizing effect, can reduce inflammatory factor response, enhance epidermal cell immune activity, and thus enhance the resistance of skin barrier.

[0017] Freezing treatment followed by ultra-high pressure treatment can help efficient wall breaking, promote the release of active ingredients, significantly improve the extraction efficiency of active ingredients, and at the same time, low temperature can inhibit enzyme activity and oxidation reaction to a certain extent, which can avoid the degradation of extracted active ingredients, and the operation is simple and suitable for industrial production.

[0018] Flash extraction is a plant active ingredient extraction technology that will not damage effective components due to high temperature or long extraction time, can maximize the retention of effective components, and has the advantages of easy operation, low cost and high extraction efficiency.

[0019] Preferably, the composition comprises the following components by weight: Artemisia capillaris extract 16 parts, L-rhamnose 9 parts, soluble protein polysaccharide 11 parts, succinoglycan 7 parts.

[0020] Preferably, the volume fraction of the ethanol solution in step B is 55-65%, and the solid-liquid ratio of Artemisia capillaris powder and ethanol solution is 1g:12-17mL.

[0021] Preferably, the freezing treatment in step B has a temperature of -20~ -15℃ and a time of 22-32 min.

[0022] Preferably, the ultra-high pressure treatment in step B has a pressure of 250-350 MPa, a number of 2-4 times, and a time of 40-60 s for each time.

[0023] Preferably, the flash extraction in step C has a voltage of 90-110 V, a number of 2-4 times, a time of 30-45 s for each time, and a rotation speed of 4000-5000 rpm.

[0024] In a second aspect, the present application provides a preparation method of the composition of the first aspect, which comprises the following steps: weighing each component according to the formula, and homogenizing after mixing to obtain the composition.

[0025] In a third aspect, the present application provides an application of the composition of the first aspect or the composition prepared by the preparation method of the second aspect in the preparation of a high-efficiency moisturizing and repairing product.

[0026] Preferably, the high-efficiency moisturizing and repairing product is a serum, a jelly, a lotion, a mask, or a cream.

[0027] The present application has the following advantages:

[0028] 1. The present application provides a high-efficiency moisturizing and repairing composition, which is composed of artemisia capillaris extract, L-rhamnose, soluble protein polysaccharide, and succinoglycan. The artemisia capillaris extract has the effects of anti-inflammation and anti-oxidation, the L-rhamnose has the properties of enhancing skin barrier, anti-inflammation, and anti-oxidation, the soluble protein polysaccharide has a long-acting moisturizing effect, and the succinoglycan has the effects of moisturizing, anti-inflammation, and enhancing the skin barrier resistance. The four components synergistically improve the effects of moisturizing, anti-inflammation, and anti-oxidation.

[0029] 2. The present application provides a preparation method of artemisia capillaris extract. The freezing and ultra-high pressure treatment are both helpful to the cell wall rupture, thereby improving the extraction efficiency and avoiding the degradation of active ingredients. In combination with flash extraction, the method has the advantages of easy operation, low cost, and high extraction efficiency, and can maximize the retention of effective components.

[0030] 3. The present application provides a preparation method of the high-efficiency moisturizing and repairing composition, which is simple and easy to operate and is conducive to industrialized production and application.

[0031] 4. The present application provides an application of the high-efficiency moisturizing and repairing composition in the preparation of a high-efficiency moisturizing and repairing product, which has a broad application prospect. DETAILED DESCRIPTION

[0032] In order to further illustrate the technical means and effects taken by the present application to achieve the predetermined object, the following detailed description is made in combination with the composition, the specific embodiments, structures, features and effects according to the present application.

[0033] The experimental methods in the following compositions without specific conditions are generally according to the conventional conditions, or according to the conditions suggested by the manufacturers. The materials, reagents, etc. used, if not specifically stated, can be obtained from commercial channels.

[0034] Part of the raw materials and sources are as follows:

[0035] Artemisia capillaris was purchased from Shanghai Xingye Biotechnology Co., Ltd.;

[0036] L-rhamnose was purchased from Shanghai Xingye Biotechnology Co., Ltd.;

[0037] Soluble protein polysaccharide was purchased from Shanghai Xingye Biotechnology Co., Ltd.;

[0038] Succinyl polysaccharide was purchased from Shanghai Xingye Biotechnology Co., Ltd.

[0039] The preparation method of Artemisia capillaris extract ① comprises the following steps:

[0040] A. Take dry Artemisia capillaris, crush, pass through a 30-mesh sieve, and obtain Artemisia capillaris powder;

[0041] B. Add an ethanol solution to the Artemisia capillaris powder, mix uniformly, perform freezing treatment, then perform ultrahigh pressure treatment, and obtain a mixture;

[0042] C. Place the mixture in a flash extractor to perform flash extraction, and obtain an extract;

[0043] D. Perform centrifugation on the extract, take the supernatant, and perform rotary evaporation until no alcohol is left, and obtain the Artemisia capillaris extract;

[0044] The volume fraction of the ethanol solution in step B is 65%, the solid-liquid ratio of the Artemisia capillaris powder and the ethanol solution is 1 g:12 mL; the freezing treatment temperature is-20℃, and the freezing treatment time is 22 min; the ultrahigh pressure treatment pressure is 250 MPa, the ultrahigh pressure treatment times is 4, and the ultrahigh pressure treatment time of each time is 60 s;

[0045] The voltage of the flash extraction in step C is 110 V, the flash extraction times is 2, the flash extraction time of each time is 30 s, and the flash extraction rotation speed is 5000 rpm.

[0046] The preparation method of Artemisia capillaris extract ② comprises the following steps:

[0047] A. Take dry Artemisia capillaris, crush, pass through a 30-mesh sieve, and obtain Artemisia capillaris powder;

[0048] B. adding an ethanol solution to the artemisia capillaris powder, mixing uniformly, performing freezing treatment, and then performing ultra-high pressure treatment to obtain a mixture;

[0049] C. placing the mixture in a flash extractor to perform flash extraction to obtain an extraction liquid;

[0050] D. performing centrifugation on the extraction liquid, taking supernatant, and performing rotary evaporation until no alcohol to obtain the artemisia capillaris extract;

[0051] In the step B, the volume fraction of the ethanol solution is 55%, the solid-liquid ratio of the artemisia capillaris powder and the ethanol solution is 1g:17mL; the freezing treatment temperature is-15℃, and the freezing treatment time is 32min; the ultra-high pressure treatment pressure is 350MPa, the number of times of ultra-high pressure treatment is 2, and the time of each ultra-high pressure treatment is 40s;

[0052] In the step C, the voltage of flash extraction is 90V, the number of times of flash extraction is 4, the time of each flash extraction is 45s, and the rotation speed of flash extraction is 4000rpm.

[0053] The preparation method of the artemisia capillaris extract ③ comprises the following steps:

[0054] A. taking dried artemisia capillaris, crushing, and passing through a 30-mesh sieve to obtain artemisia capillaris powder;

[0055] B. adding an ethanol solution to the artemisia capillaris powder, mixing uniformly, performing freezing treatment, and then performing ultra-high pressure treatment to obtain a mixture;

[0056] C. placing the mixture in a flash extractor to perform flash extraction to obtain an extraction liquid;

[0057] D. performing centrifugation on the extraction liquid, taking supernatant, and performing rotary evaporation until no alcohol to obtain the artemisia capillaris extract;

[0058] In the step B, the volume fraction of the ethanol solution is 55%, the solid-liquid ratio of the artemisia capillaris powder and the ethanol solution is 1g:17mL; the freezing treatment temperature is-15℃, and the freezing treatment time is 32min; the ultra-high pressure treatment pressure is 350MPa, the number of times of ultra-high pressure treatment is 2, and the time of each ultra-high pressure treatment is 40s;

[0059] In the step C, the voltage of flash extraction is 90V, the number of times of flash extraction is 4, the time of each flash extraction is 45s, and the rotation speed of flash extraction is 4000rpm.

[0060] The artemisia capillaris extract ④ differs from the artemisia capillaris extract ③ only in that the volume fraction of the ethanol solution in the preparation step B of the artemisia capillaris extract ④ is 50%, and other conditions are consistent with those of the artemisia capillaris extract ③.

[0061] Artemisia capillaris extract ⑤ is only different from artemisia capillaris extract ③ in that the volume fraction of the ethanol solution in step B of the preparation of artemisia capillaris extract ⑤ is 70%, and other conditions are consistent with artemisia capillaris extract ③.

[0062] Artemisia capillaris extract ⑥ is only different from artemisia capillaris extract ③ in that the pressure of the ultrahigh pressure treatment in step B of the preparation of artemisia capillaris extract ⑥ is 200 MPa, and other conditions are consistent with artemisia capillaris extract ③.

[0063] Artemisia capillaris extract ⑦ is only different from artemisia capillaris extract ③ in that the pressure of the ultrahigh pressure treatment in step B of the preparation of artemisia capillaris extract ⑦ is 400 MPa, and other conditions are consistent with artemisia capillaris extract ③.

[0064] Artemisia capillaris extract ⑧ is only different from artemisia capillaris extract ③ in that step B of the preparation of artemisia capillaris extract ⑧ is different, and other conditions are consistent with artemisia capillaris extract ③.

[0065] Step B is specifically as follows: ethanol solution is added to artemisia capillaris powder, mixed uniformly, and soaked at room temperature for 6 h to obtain a mixture; wherein the volume fraction of the ethanol solution in step B is 60%, and the solid-liquid ratio of artemisia capillaris powder and ethanol solution is 1 g:14 mL.

[0066] The component composition (weight fraction) of the high-efficiency moisturizing repair composition of examples 1-3 in the application is shown in Table 1 below, and each component is weighed according to the formula amount, mixed and homogenized to obtain each composition.

[0067] Table 1 Component and weight fraction of the high-efficiency moisturizing repair composition of examples 1-3

[0068] Example Artemisia capillaris extract Artemisia capillaris extract L-rhamnose Soluble protein polysaccharide Succinoglycan 1 ① 14 11 9 9 2 ② 18 7 13 5 3 ③ 16 9 11 7

[0069] Comparative example 1: compared with example 3, the difference is that no artemisia capillaris extract is added in comparative example 1, and the missing amount is filled with L-rhamnose, soluble protein polysaccharide and succinoglycan at a weight ratio of 9:11:7, and other conditions are consistent with example 3.

[0070] Comparative example 2: compared with example 3, the difference is that no L-rhamnose is added in comparative example 2, and the missing amount is filled with artemisia capillaris extract, soluble protein polysaccharide and succinoglycan at a weight ratio of 16:11:7, and other conditions are consistent with example 3.

[0071] Comparative example 3: compared with example 3, the difference is that no soluble protein polysaccharide is added in comparative example 3, and the missing amount is filled with artemisia capillaris extract, L-rhamnose and succinoglycan at a weight ratio of 16:9:7, and other conditions are consistent with example 3.

[0072] Comparative Example 4: Compared with Example 3, the difference is that no succinoglycan is added in Comparative Example 4, and the missing amount is supplemented with artemisia capillaris extract, L-rhamnose and soluble protein polysaccharide in a weight ratio of 16:9:11, and other conditions are consistent with Example 3.

[0073] Comparative Example 5: Compared with Example 3, the difference is that artemisia capillaris extract (IV) is used instead of artemisia capillaris extract (III) in Comparative Example 5, and other conditions are consistent with Example 3.

[0074] Comparative Example 6: Compared with Example 3, the difference is that artemisia capillaris extract (V) is used instead of artemisia capillaris extract (III) in Comparative Example 6, and other conditions are consistent with Example 3.

[0075] Comparative Example 7: Compared with Example 3, the difference is that artemisia capillaris extract (VI) is used instead of artemisia capillaris extract (III) in Comparative Example 7, and other conditions are consistent with Example 3.

[0076] Comparative Example 8: Compared with Example 3, the difference is that artemisia capillaris extract (VII) is used instead of artemisia capillaris extract (III) in Comparative Example 8, and other conditions are consistent with Example 3.

[0077] Comparative Example 9: Compared with Example 3, the difference is that artemisia capillaris extract (VIII) is used instead of artemisia capillaris extract (III) in Comparative Example 9, and other conditions are consistent with Example 3.

[0078] Test Example 1 Hyaluronidase Inhibition Experiment

[0079] Hyaluronic acid is a strong moisturizer that can attract and lock in moisture, keeping the skin moist, improving dry, rough and water-deficient skin conditions; and hyaluronic acid can also enhance the biological activity of cells, and has a strong synergistic effect on repairing and protecting the skin. Hyaluronidase is a class of enzymes that can hydrolyze hyaluronic acid, which exists widely in human tissues, and its increased activity can lead to the decomposition of hyaluronic acid, thereby reducing the moisturizing ability of the skin. Inhibiting the activity of hyaluronidase can slow down the decomposition of hyaluronic acid, thereby maintaining the content of hyaluronic acid in the skin and enhancing the moisturizing and repairing ability of the skin. Therefore, this test tests the inhibition rate of the composition on hyaluronidase, and then evaluates the moisturizing effect of the high-efficiency moisturizing and repairing composition prepared in Examples 1-3 and Comparative Examples 1-9.

[0080] Test sample: high-efficiency moisturizing and repairing composition prepared in Examples 1-3 and Comparative Examples 1-9 of the present application, respectively diluted with DMSO to a concentration of 4.5% for use;

[0081] The test specifically includes the following steps:

[0082] 1.1 Take four groups of clean test tubes, respectively, labeled A, B, C, D, in A, B test tube each add 50 μL of the sample to be tested, in C, D test tube each add 50 μL of distilled water, in A, C test tube each add 50 μL of 500 U / mL hyaluronidase solution, in B, D test tube each add 50 μL of pH = 5.6 acetic acid buffer, the test tube is placed in 37℃ incubator incubation 20 min;

[0083] 1.2 To the four groups of test tubes respectively add 10 μL of 2.5 mol / L CaCl2 solution, then placed in 37℃ incubator incubation 20 min;

[0084] 1.3 To A, C test tube add 50 μL of 0.5 mg / mL sodium hyaluronate solution, to B, D test tube add 50 μL of pH = 5.6 acetic acid buffer, the test tube is placed in 37℃ incubator incubation 40 min, then take out room temperature for 10 min;

[0085] 1.4 To the four groups of test tubes respectively add 50 μL of distilled water, 10 μL of 5 mol / L NaOH solution, 50 μL of acetylacetone solution, the test tube is placed in a water bath for 30 min, then ice bath 10 min, finally at room temperature for 10 min;

[0086] 1.5 To each test tube add 100 μL of P-DAB reagent, then use the enzyme marker to measure the absorbance at 530 nm;

[0087] The calculation method of hyaluronidase inhibition rate is as follows:

[0088] ;

[0089] In the formula: A is the OD value of the sample solution; B is the OD value of the sample blank; C is the OD value of the control sample solution; D is the OD value of the control sample blank; each group is determined in triplicate, and the data is presented as an average value;

[0090] The hyaluronidase inhibition rate data is shown in Table 2 below.

[0091] Table 2 Hyaluronidase inhibition rate data

[0092] Group Hyaluronidase inhibition rate (%) Example 1 93.36 Example 2 90.78 Example 3 94.02 Comparative Example 1 68.17 Comparative Example 2 76.42 Comparative Example 3 72.37 Comparative Example 4 74.19 Comparative Example 5 86.24 Comparative Example 6 87.57 Comparative Example 7 85.46 Comparative Example 8 88.29 Comparative Example 9 80.35

[0093] Test Example 2 Cell scratch test

[0094] Human immortalized epidermal cell line (HaCaT) is derived from keratinocytes of adult skin, which has the characteristics of adherent growth, morphological stability, good differentiation ability, etc. Because it can obtain unlimited proliferation ability through spontaneous mutation, it is an important in vitro model for studying human epidermal function, skin disease mechanism, inflammatory response, regeneration and repair, as well as cosmetic and drug evaluation.

[0095] Cell migration refers to the movement of cells after receiving a migration signal or sensing certain substances, which is involved in many physiological or pathological activities such as immunity, tumor metastasis, inflammation, and damage repair.

[0096] The cell scratch test is to artificially create a blank area on a fused monolayer of cells, which is called "scratch / wound". The cells at the edge of the scratch will gradually enter the blank area to heal the "scratch / wound". During the cell migration process, images are captured at regular intervals. By measuring the distance between scratches at different time points and calculating the difference, the migration ability of cells can be judged. It can be used to evaluate the influence of exogenous factors such as drugs and genes on cell migration, repair and interaction.

[0097] Test substances: high-efficiency moisturizing repair compositions prepared in Examples 1-3 and Comparative Examples 1-9;

[0098] Test substance treatment: the test substances were diluted with DMEM culture solution to a concentration of 0.005 mg / mL for use;

[0099] The test specifically includes the following steps:

[0100] 2.1 Draw horizontal lines evenly behind the 6-well plate with a marker pen, one horizontal line every 0.5 cm, crossing the wells, and 5 lines per well;

[0101] 2.2 Take HaCaT cells in the logarithmic growth phase, digest them into a single cell suspension with 0.25% trypsin, and inoculate HaCaT cells with a concentration of 6x10 5 cells / mL into the 6-well plate at 2 mL / well. Add DMEM culture medium containing 10% FBS, and incubate at 37°C in a 5% CO2 cell incubator for 24 h until a fused cell monolayer is formed in the 6-well plate. At this time, use a 200 μL gun head to vertically scratch the horizontal lines behind the 6-well plate, scratch two lines in each well, rinse with PBS for 3 times, remove the scratched cells, add 1.8 mL of DMEM culture medium without FBS, and take a photo as the initial image of the cell scratch test;

[0102] 2.3 Add 200 μL of the test substance to each well, and continue to incubate at 37°C in a 5% CO2 cell incubator for 24 h. Take it out and take a photo as the 24 h image of the cell scratch test. The blank control group is replaced with 200 μL of PBS instead of the test substance. Each test substance group and the blank control group are set up with 5 replicate wells.

[0103] 2.4 Perform picture area analysis on the initial image and the 24 h image of the cell scratch test using Image J software to obtain the initial scratch area and the 24 h scratch area, respectively.

[0104] 2.5 Calculate the cell migration rate according to the following formula:

[0105] ;

[0106] 2.6 Data processing: data statistics were processed by SPSS statistical software, and all measurement data were expressed as mean ± standard deviation. Statistical analysis was performed by t test, and P<0.05 was statistically significant.

[0107] The results of the cell scratch test are shown in Table 3 below, and the higher the cell migration rate, the better the repair effect of the high-efficiency moisturizing repair composition prepared in the application on skin barrier damage.

[0108] Table 3 Results of cell scratch test

[0109] Group Cell migration rate (%) Blank control 15.33±1.72 Example 1 65.91 ± 5.24 * ]] Example 2 63.32 ± 6.01 * ]] Example 3 68.18 ± 6.29 * ]] Comparative Example 1 40.13 ± 3.87 *△ ]] Comparative Example 2 50.86 ± 5.12 *△ ]] Comparative Example 3 46.25 ± 4.53 *△ ]] Comparative Example 4 48.09 ± 4.61 *△ ]] Comparative Example 5 56.28 ± 5.24 *△ ]] Comparative Example 6 59.74 ± 5.86 *△ ]] Comparative Example 7 57.14 ± 5.78 *△ ]] Comparative Example 8 58.53 ± 5.66 *△ ]] Comparative Example 9 52.31 ± 5.06 *△ ]]

[0110] Note: compared with the blank group, P<0.05 is indicated by “*”; compared with Example 3, P<0.05 is indicated by “△”.

[0111] Compared with Example 3, Comparative Examples 1-4 do not add one of Artemisia capillaris extract, L-rhamnose, soluble protein polysaccharide and succinoglycan, respectively, and the preparation process of Artemisia capillaris extract in Comparative Examples 5-9 is different from that of Artemisia capillaris extract in Example 3; among them, Comparative Examples 5-6 use different volume fractions of ethanol solution in preparation step B, Comparative Examples 7-8 use different pressures for ultra-high pressure treatment in preparation step B, and Comparative Example 9 uses long-time room temperature soaking instead of the freezing treatment step and the ultra-high pressure treatment step.

[0112] From the hyaluronidase inhibition rate and cell migration rate data of Examples 1-3 and Comparative Examples 1-4 in Tables 2-3, it can be seen that Examples 1-3, which are compounded by selecting Artemisia capillaris extract, L-rhamnose, soluble protein polysaccharide and succinoglycan, have higher hyaluronidase inhibition rate and cell migration rate, and the four components of Artemisia capillaris extract, L-rhamnose, soluble protein polysaccharide and succinoglycan have synergistic effect, so that the moisturizing repair ability of the composition is enhanced.

[0113] From the data of Example 3 and Comparative Examples 5-9 in Tables 2-3, it can be seen that the preparation process of Artemisia capillaris extract will also significantly affect the extraction efficiency and effect of Artemisia capillaris extract, thereby affecting the hyaluronidase inhibition rate and cell migration rate of the composition, i.e. the moisturizing repair effect of the composition.

[0114] In summary, the preparation method of the extract of Artemisia capillaris provided by the application adopts freezing, ultrahigh pressure treatment and flash extraction, has the advantages of improving the extraction efficiency and avoiding degradation of active ingredients, is easy to operate, has low cost, and can maximize the retention of effective components; the high-efficiency moisturizing and repairing composition provided by the application is composed of the extract of Artemisia capillaris, L-rhamnose, soluble protein polysaccharide and succinoglucan, has good moisturizing and repairing effects and is mild and non-irritating, and has a wide application prospect.

[0115] Test Example 3: Cytotoxicity test

[0116] Tested substances: moisturizing and repairing compositions prepared in Examples 1-3;

[0117] The test specifically includes the following steps:

[0118] 3.1 Take human immortalized epidermal cell line (HaCaT) in a stable growth state, adjust the cell concentration to 1×10 5 cells / mL, inoculate 100 μL per well into a 96-well cell culture plate (except for the outermost wells and blank control wells); add 200 μL of PBS to the outermost wells of the cell culture plate to reduce evaporation of the culture medium; place the 96-well cell culture plate in a carbon dioxide incubator at 37℃±1℃ and a CO2 concentration of 5.0%±1%, and culture until the cells are fused to more than 90%;

[0119] 3.2 Dilute the tested substances with PBS to a concentration of 10 mg / L for use, set up a blank control group (use PBS instead of the tested substances) and a tested substance group, 100 μL per well, set up 6 replicate wells for each group, and present the data as an average value; start timing from the time when the tested substances (tested substance group) and PBS (blank control) are added, keep the time interval between adding samples in every two columns consistent, and ensure that the intervention time of each group on the cells is 5 min;

[0120] 3.3 After 5 min of intervention, sequentially suck the tested substances, add PBS to each well to wash the tested substances, and wash twice; when sucking the liquid in the wells with a vacuum suction pump or a row gun, operate carefully to avoid sucking the living cells and affecting the experimental results;

[0121] 3.4 Add 100 μL of DMEM culture medium and 10 μL of CCK-8 solution to each well, and place in the incubator for about 2 h;

[0122] 3.5 Measure the absorbance (OD) value at a wavelength of 450 nm, and calculate the cell survival rate according to the following formula:

[0123] ;

[0124] The test results are shown in Table 4;

[0125] Table 4 Cell survival rate data

[0126] Group Cell survival rate (%) Example 1 96.3 Example 2 94.6 Example 3 98.1

[0127] The cell survival rates of Examples 1-3 are all higher than 94%, indicating that the high-efficiency moisturizing repair compositions provided by the present application do not have toxic effects on cells and have high safety.

[0128] The above is only the preferred composition of the present application, and does not limit the present application in any form. Although the present application has been disclosed as the preferred composition above, it is not intended to limit the present application. Any person skilled in the art can make slight changes or modifications to the equivalent composition by using the disclosed technical content above without departing from the technical solution range of the present application. Any modification, equivalent change and modification of the above composition made according to the technical essence of the present application without departing from the technical solution content of the present application still belongs to the technical solution range of the present application.

Claims

1. A high efficiency moisturizing repair composition characterized in that, The composition comprises the following components by weight parts: artemisia capillaris extract 14-18 parts, L-rhamnose 7-11 parts, soluble proteoglycan 9-13 parts, succinyl polysaccharide 5-9 parts; The preparation method of the artemisia capillaris extract comprises the following steps: A. Take dry artemisia capillaris, crush, pass through a 30-mesh sieve, and obtain artemisia capillaris powder; B. Add an ethanol solution to the artemisia capillaris powder, mix uniformly, perform freezing treatment, then perform ultrahigh pressure treatment, and obtain a mixture; C. Place the mixture in a flash extractor to perform flash extraction, and obtain an extraction liquid; D. Perform centrifugation on the extraction liquid, take the supernatant, and perform rotary evaporation until no alcohol is present, and obtain the artemisia capillaris extract; The soluble proteoglycan is a proteoglycan solution extracted from salmon nasal cartilage tissue, which has one or more covalent bonds that can be connected to mucopolysaccharide substances, and has a long-acting moisturizing effect; The volume fraction of the ethanol solution in step B is 55-65%, and the solid-liquid ratio of the artemisia capillaris powder and the ethanol solution is 1g:12-17mL; The pressure of the ultrahigh pressure treatment in step B is 250-350MPa, the number of ultrahigh pressure treatments is 2-4 times, and the time of each ultrahigh pressure treatment is 40-60s.

2. The composition of claim 1, wherein The composition comprises the following components by weight parts: artemisia capillaris extract 16 parts, L-rhamnose 9 parts, soluble proteoglycan 11 parts, and succinyl polysaccharide 7 parts.

3. The composition of claim 1, wherein The freezing temperature in step B is-20~-15℃, and the freezing time is 22-32min.

4. The composition of claim 1, wherein The voltage of the flash extraction in step C is 90-110V, the number of flash extractions is 2-4 times, the time of each flash extraction is 30-45s, and the rotation speed of the flash extraction is 4000-5000rpm.

5. A process for the preparation of a composition according to any one of claims 1 to 4, characterised in that, The preparation method comprises the following steps: weighing each component according to the formula amount, mixing, and homogenizing to obtain the composition.

6. Use of the composition of any one of claims 1-4 or the composition prepared by the preparation method of claim 5 in the preparation of a high-efficiency moisturizing and repairing product.

7. The use of a composition according to claim 6, characterized in that The high-efficiency moisturizing and repairing product is a serum, a jelly, a lotion, a mask, or a cream.