Bactericidal composition for preventing and treating rape pod black spot and application thereof
The combination of fluazifop-butyl and propiconazole solved the problem of prevention and control of rapeseed silique black spot disease, achieved efficient and environmentally friendly prevention and control effects, reduced farmers' drug costs and improved rapeseed quality.
Patent Information
- Application Number
- CN202511066464.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-07-31
- Publication Date
- 2025-10-10
AI Technical Summary
In the existing technology, rapeseed pod black spot control agents have problems such as increased drug resistance, short duration of effect, and high environmental pressure. The sole use of fungicides leads to insufficient control effect and is harmful to the soil environment.
A fungicide composition containing fluopicolide and propiconazole as active ingredients in a mass ratio of 1-3:1-3 is prepared into a solution-type pesticide for preventing and controlling rapeseed pod black spot disease.
It significantly improved the prevention and control effect, up to 71.88%, reduced farmers' pesticide costs, reduced the environmental impact on the soil, and improved the quality of rapeseed.
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Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of pesticides, and in particular to a fungicide composition for preventing and treating rapeseed silique black spot disease and an application thereof. Background Art
[0002] Black spot disease of rapeseed siliques is caused by a fungus called Alternaria. Besides infecting rapeseed, black spot disease also affects crops of the Brassica family, such as radish, cauliflower, mustard, and cabbage. It is most likely to develop when the temperature is between 15 and 25°C and the field humidity is high. Currently, black spot disease of rapeseed occurs in all rapeseed-producing areas, often occurring when the siliques develop and mature, and occurs on leaves, stems, and siliques. Infected leaves show circular brown lesions with concentric rings surrounded by a yellow halo. These lesions crack and perforate when dry, and produce black mold when wet. Infected stems show spindle-shaped or oval lesions, which are brown in the early stages, white in the middle later, and dark brown at the edges. The lesions can surround side branches, causing branch dieback. Infected siliques appear as circular or oval black spots, and under high humidity conditions, the surface of the siliques is densely covered with a layer of black mold. Rapeseed silique black spot disease can significantly reduce photosynthesis in rapeseed leaves and siliques, especially during silique development and grain filling. This disease can severely impact rapeseed thousand-grain weight, oil content, and quality, resulting in a 20% to 50% yield reduction, posing a serious threat to the safe production of the rapeseed industry.
[0003] Currently, production mainly relies on single-dose fungicides such as triazoles (such as tebuconazole) and strobilurins (such as azoxystrobin). However, long-term single-drug use leads to the following problems:
[0004] Increasing drug resistance: The sensitivity of pathogens to triazoles such as propiconazole decreases year by year, and the prevention efficiency is less than 50%.
[0005] Short duration of effectiveness: Conventional pesticides have weak resistance to rain erosion and require frequent application, which increases costs.
[0006] Environmental pressure: High-dose use causes imbalance in soil microbial communities and increases the risk of residues.
[0007] Scientifically formulated fungicides are a key strategy for addressing resistance and improving efficacy. By synergizing multiple sites of action, they can delay the development of resistance, expand the antimicrobial spectrum, and reduce the dosage required for each single agent.
[0008] Pydiflumetofen boasts high efficacy, broad spectrum, systemic activity, and resistance to rainwater washout. It exhibits excellent activity against a variety of diseases, including gray mold, wheat head blight, sclerotinia, early blight, leaf spot, and black spot. It disrupts energy metabolism by affecting complex II in the pathogen's respiratory electron transport chain, thereby inhibiting mitochondrial function and blocking energy production, leading to pathogen death. Propiconazole is a highly effective triazole fungicide that is absorbed by the roots, stems, and leaves of crops and rapidly transports upward within the plant. It controls diseases caused by imperfect fungi, ascomycetes, and basidiomycetes, and is particularly effective against wheat powdery mildew, soybean rust, mango anthracnose, rice sphaeroides, and banana leaf spot. Its mechanism of action is to inhibit sterol biosynthesis, disrupting pathogen cell membrane function and ultimately leading to cell death, achieving a fungicidal and disease-preventing effect.
[0009] Mixing certain fungicides can overcome or reduce crop damage. At the same time, due to the differences in chemical composition and physical and chemical properties of various fungicides, the sterilization range is also different. Some fungicides have a narrow sterilization range, but have unique effects; some fungicides have a wide sterilization spectrum and can simultaneously prevent and treat multiple diseases in the field. Studies have shown that long-term use of a single fungicide is not only prone to resistance and phytotoxicity, but also has a greater impact on the soil environment. The compounding of fungicides is widely considered to be one of the important measures for the rational use of fungicides. After compounding, it can reduce the generation of resistance, reduce the phytotoxicity of fungicides to economic crops, reduce the impact on the soil, and thus improve economic benefits. Therefore, a compound fungicide composition that can effectively prevent and treat rapeseed pod black spot is developed in order to achieve the above-mentioned effects. Summary of the Invention
[0010] In view of the above technical problems, the present invention provides a fungicide composition for preventing and treating rapeseed silique black spot disease and its application.
[0011] The present invention adopts the following technical solutions: The invention provides a fungicide composition for preventing and treating rapeseed silique black spot disease. The composition comprises pentocyclamide and propiconazole as active ingredients, and the mass ratio of pentocyclamide to propiconazole is 1-3:1-3.
[0012] The present invention provides a fungicide composition, which is prepared by mixing pentocyclamide and propiconazole in a specific ratio. By using the fungicide composition, a good prevention and treatment effect on rapeseed silique black spot disease can be achieved.
[0013] The present invention also provides use of the bactericidal composition in preparing a pesticide for preventing and treating rapeseed silique black spot disease.
[0014] Furthermore, the pesticide is in the form of a solution.
[0015] Furthermore, the solvent of the pesticide is water.
[0016] Furthermore, the concentration of pentoquinoxaline in the pesticide is 300 mg / L to 500 mg / L.
[0017] The present invention also provides use of the bactericidal composition in preparing a bacteriostatic agent, wherein the bacteriostatic agent is used for inhibiting Alternaria fungi.
[0018] Furthermore, the dosage form of the antibacterial agent is a solution dosage form.
[0019] Furthermore, the solvent of the antibacterial agent is water.
[0020] The present invention has the following beneficial effects: Experiments have demonstrated that the fungicidal combination of fluopicolide and propiconazole in the present invention has a significant control effect on rapeseed silique black spot, with the control effect reaching up to 71.88%, an increase of over 60% compared to the control effect of a single agent (41% to 44%). This combination effectively prevents and controls the harmful effects of rapeseed silique black spot, providing a new option for the prevention and control of rapeseed silique black spot. The fungicidal combination of fluopicolide and propiconazole provided by the present invention overcomes the technical bottlenecks of existing single-agent and combined products. Its water-based formulation aligns with pesticide reduction policies, significantly reducing farmers' pesticide costs and improving rapeseed quality, significantly contributing to national oilseed security. DETAILED DESCRIPTION
[0021] The present invention is described in detail below in conjunction with schemes and specific examples, but should not be construed as limiting the present invention. Unless otherwise specified, the technical means used in the following examples are conventional means well known to those skilled in the art, and the materials, reagents, etc. used in the following examples, unless otherwise specified, can be obtained from commercial sources.
[0022] The pentocyclamide suspension concentrate was purchased from Syngenta (Nantong) Crop Protection Co., Ltd., and the pesticide registration number is PD20210035.
[0023] Propiconazole emulsifiable concentrate was purchased from Syngenta (Suzhou) Crop Protection Co., Ltd., and the pesticide registration number is PD20060028.
[0024] Example 1: Composition formulation optimization and verification.
[0025] 1. Experimental setup.
[0026] Group A is a working solution in which the mass ratio of fluopicolide to propiconazole is 1:1. The preparation method is to put 20L of water in a preparation barrel, add 50ml of fluopicolide suspension with a fluopicolide concentration of 200g / L, and then add 40ml of propiconazole emulsifiable concentrate with a propiconazole concentration of 250g / L and mix well, add water to make the volume to 30L and stir thoroughly to obtain the working solution of Group A.
[0027] Group B is a working solution in which the mass ratio of fluopicolide to propiconazole is 1:2. The preparation method is to put 20L of water in the preparation barrel, add 33ml of fluopicolide suspension with a fluopicolide concentration of 200g / L, and then add 53ml of propiconazole emulsifiable concentrate with a propiconazole concentration of 250g / L and mix well, add water to make the volume to 30L and stir thoroughly to obtain the working solution of Group B.
[0028] The mass ratio of fluopicolide and propiconazole in group C is 1:3. The preparation method is to put 20L of water in the preparation barrel, add 25ml of fluopicolide suspension with a fluopicolide concentration of 200g / L, and then add 60ml of propiconazole emulsifiable concentrate with a propiconazole concentration of 250g / L and mix well, add water to make the volume to 30L and stir well to obtain the working solution of group C.
[0029] The mass ratio of fluopicolide and propiconazole in group D is 1:5. The preparation method is to put 20L of water in the preparation barrel, add 17ml of fluopicolide suspension with a fluopicolide concentration of 200g / L, and then add 68ml of propiconazole emulsifiable concentrate with a propiconazole concentration of 250g / L and mix well, add water to make the volume to 30L and stir well to obtain the working solution of group D.
[0030] The mass ratio of fluopicolide and propiconazole in group E is 1:10. The preparation method is to put 20L of water in the preparation barrel, add 9ml of fluopicolide suspension with a fluopicolide concentration of 200g / L, and then add 72ml of propiconazole emulsifiable concentrate with a propiconazole concentration of 250g / L and mix well, add water to make the volume to 30L and stir well to obtain the working solution of group E.
[0031] The mass ratio of fluopicolide and propiconazole in group F is 2:1. The preparation method is to put 20L of water in the preparation barrel, add 67ml of fluopicolide suspension with a fluopicolide concentration of 200g / L, and then add 27ml of propiconazole emulsifiable concentrate with a propiconazole concentration of 250g / L and mix well, add water to make the volume to 30L and stir thoroughly to obtain the working solution of group F.
[0032] The mass ratio of fluopicolide and propiconazole in group G is 3:1. The preparation method is to put 20L of water in the preparation barrel, add 75ml of fluopicolide suspension with a fluopicolide concentration of 200g / L, and then add 20ml of propiconazole emulsifiable concentrate with a propiconazole concentration of 250g / L and mix well, add water to make the volume to 30L and stir thoroughly to obtain the working solution of group G.
[0033] The mass ratio of fluopicolide and propiconazole in group H is 5:1. The preparation method is to put 20L of water in the preparation barrel, add 83ml of fluopicolide suspension with a fluopicolide concentration of 200g / L, and then add 13ml of propiconazole emulsifiable concentrate with a propiconazole concentration of 250g / L and mix well, add water to make the volume to 30L and stir thoroughly to obtain the working solution of group H.
[0034] Group I is a working solution in which the mass ratio of fluopicolide to propiconazole is 10:1. The preparation method is to put 20L of water in a dispensing barrel, add 91ml of fluopicolide suspension with a fluopicolide concentration of 200g / L, and then add 7ml of propiconazole emulsifiable concentrate with a propiconazole concentration of 250g / L and mix well, add water to make the volume to 30L and stir thoroughly to obtain the working solution of Group I.
[0035] Control group 1 was prepared by adding only propiconazole. The preparation method was as follows: 20 L of water was placed in a preparation barrel, 80 ml of propiconazole emulsifiable concentrate with a propiconazole concentration of 250 g / L was added and mixed, water was added to make the volume up to 30 L and the mixture was stirred thoroughly to obtain a working solution with a propiconazole concentration of 666 mg / L.
[0036] The control group 2 was prepared by adding only fluopicolide. The preparation method was as follows: 20 L of water was placed in a preparation barrel, 100 ml of a fluopicolide suspension with a fluopicolide concentration of 200 g / L was added and mixed, water was added to make the volume to 30 L and the mixture was stirred thoroughly to obtain a working solution with a fluopicolide concentration of 666 mg / L.
[0037] The blank group was sprayed with water only.
[0038] 2. Experimental methods
[0039] The experiment was conducted at the Yichun Academy of Sciences in Yuanzhou District, Yichun City (latitude: 27.785366, longitude 114.416350). The experimental fields are flat, uniformly fertile, and contiguous, with frequent outbreaks of black spot. The experimental crop was rapeseed (Ganyouza 8). Twelve treatments, each with three replicates, were used, resulting in a total of 45 plots. Each plot was arranged in a randomized block arrangement, with an area of 20 square meters. Seeds were sown manually on October 10, 2024, with a seeding rate of 18g per plot.
[0040] The test target was rapeseed silique black spot disease. Application was carried out using a knapsack electric sprayer at a water rate of 30 kg / mu (approximately 1.5 lb / mu). Application took place on April 2, 2025 (the early stage of rapeseed silique formation) on sunny days with temperatures ranging from 11°C to 23°C. A silique black spot disease survey was conducted five weeks after application. Each plot was sampled using a five-point sampling method, with 20 rapeseed plants surveyed at each point, for a total of 100 plants. The number of diseased plants at each level was recorded, and the disease index and control efficacy were calculated.
[0041] Disease severity grading standards: Level 0: There are no disease spots on the whole plant.
[0042] Level 1: Less than 1 / 3 of the branches of the plant are diseased or the length of the lesions on the main stem does not exceed 3 cm.
[0043] Level 2: 1 / 3 to 2 / 3 of the branches of the plant are diseased, or less than 1 / 3 of the branches are diseased, but the length of the lesions on the main stem exceeds 3 cm.
[0044] Level 3: More than 2 / 3 of the branches of the plant are diseased, or less than 2 / 3 of the branches are diseased, but the lesions on the main stem are more than 3 cm.
[0045] Level 4: The whole plant is diseased.
[0046] Calculation method of drug efficacy: Disease index (%) = [(number of diseased plants at each level × corresponding level) / (total number of plants surveyed × 4)] × 100, control effect (%) = [(disease index of blank group - disease index of experimental group) / disease index of blank group] × 100.
[0047] 3. Experimental results.
[0048] The control effects of different pesticides on rapeseed silique black spot disease are shown in Table 1 below.
[0049] Table 1 The control effect of different pesticides on rapeseed pod black spot Note: Different lowercase letters indicate significant differences among groups, p<0.05.
[0050] The results of the control effect investigation showed that the mass ratio of fluopicolide to propiconazole was 1:1, with the best control effect against rapeseed pod black spot, reaching 71.88%, which was significantly better than the mixed pesticides of groups D, E, H, and I, and the single-dose varieties of control groups 1 and 2. The control effects of groups D and H were 55.70% and 56.44%, respectively, which were significantly better than the mixed pesticides of groups E and I, and the single-dose varieties of control groups 1 and 2. The control effect of the mixed pesticide of groups E and I was not significantly different from that of the single-dose varieties of control groups 1 and 2. Field observations 3 and 7 days after application showed that rapeseed in the experimental area grew normally, and no symptoms of phytotoxicity were observed with the above-mentioned pesticides, indicating that the test pesticides were safe for rapeseed under the experimental conditions.
[0051] This invention demonstrates for the first time that a combination of pentocyclamide and propiconazole in a 1-3:1-3 ratio demonstrates excellent synergistic efficacy against rapeseed silique black spot, overcoming the technical bottlenecks of existing single-agent and combined products. This water-based formulation, aligned with pesticide reduction policies, can significantly reduce pesticide costs for farmers and improve rapeseed quality, significantly contributing to national oilseed security.
[0052] It should be noted that when the claims of the present invention involve numerical ranges, it should be understood that the two endpoints of each numerical range and any numerical value between the two endpoints can be selected. In order to avoid redundancy, the present invention describes preferred embodiments.
[0053] Although the preferred embodiments of the present invention have been described, those skilled in the art may make additional changes and modifications to these embodiments once they have learned the basic creative concept. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications that fall within the scope of the present invention.
[0054] Obviously, those skilled in the art may make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if such changes and modifications fall within the scope of the claims and their equivalents, the present invention is intended to include such changes and modifications.
Claims
1. A fungicidal composition for preventing and treating rapeseed pod black spot disease, characterized in that: The invention takes pentocyclamide and propiconazole as active ingredients, and the mass ratio of pentocyclamide to propiconazole is 1-3:1-3.
2. Use of the bactericidal composition according to claim 1 in the preparation of a pesticide for preventing and treating rapeseed pod black spot disease.
3. Use of the bactericidal composition according to claim 2 in the preparation of a pesticide for preventing and treating rapeseed pod black spot disease, characterized in that: The dosage form of the pesticide is a solution dosage form.
4. Use of the bactericidal composition according to claim 3 in preparing a product for preventing and treating rapeseed silique black spot disease, characterized in that: The solvent of the pesticide is water.
5. Use of the bactericidal composition according to claim 3 in preparing a product for preventing and treating rapeseed silique black spot disease, characterized in that: The concentration of pentocyclamide in the pesticide is 300 mg / L to 500 mg / L.
6. Use of the bactericidal composition according to claim 1 in the preparation of a bacteriostatic agent, characterized in that: The bacteriostatic agent is used for inhibiting Alternaria fungi.
7. Use of the bactericidal composition according to claim 6 in the preparation of a bacteriostatic agent, characterized in that: The dosage form of the bacteriostatic agent is a solution dosage form.
8. Use of the bactericidal composition according to claim 7 in the preparation of a bacteriostatic agent, characterized in that: The solvent of the bacteriostatic agent is water.