Preparation process of selenium-wolfberry liver-tonifying mixture

Abstract

The invention provides a preparation process of a selenium-wolfberry liver-tonifying mixture, which comprises the following steps: decocting wolfberry with water, adding tea leaves for decoction, adding the tea leaves weighed according to a mass ratio into the wolfberry water decoction, decocting to form a primary medicine liquid, standing the medicine liquid, filtering to form a filtrate, concentrating the filtrate under reduced pressure to form a concentrated solution, carrying out alcohol precipitation on the concentrated solution, standing and filtering, and thus obtaining the selenium-wolfberry liver-tonifying mixture. Performing vacuum concentration on the filtrate to remove ethanol to form ointment, adding honey, royal jelly and purified water to prepare liquid with the ointment, filtering the liquid medicine, selecting, washing and drying a bottle, filling and sealing, sterilizing, performing lamp inspection, and packaging to form a bottled mixture.

Description

Technical Field

[0001] The invention relates to the technical field of medicine preparation, in particular to a preparation process of a selenium-wolfberry liver-tonifying mixture. Background Art

[0002] Cancer patients develop liver and kidney yin deficiency syndrome after radiotherapy and chemotherapy, with symptoms such as dizziness, dry mouth and throat, soreness of waist and knees, poor sleep and tinnitus. For this, selenium and wolfberry liver-tonifying mixture is needed for auxiliary treatment to nourish the liver and kidneys, nourish yin and promote fluid production.

[0003] The benefits of wolfberry include nourishing the liver, kidneys, and lungs. It can treat liver and kidney deficiency, dizziness, clear vision, soreness of the waist and knees, impotence, coughing due to consumptive diseases, and thirst. Modern research shows that wolfberry has the following pharmacological effects: 1. Impact on immune function: 1.1. Effect of Lycium barbarum polysaccharide on 3H-TdR incorporation into mouse spleen lymphocytes and Ts cells: 1.1.1. Effect on 3H-TdR Incorporation in Human Splenic Lymphocytes: Intraperitoneal administration of Lycium barbarum polysaccharide (LBP) at 5 mg / kg and 10 mg / kg / day for 7 days significantly increased ConA-induced 3H-TdR incorporation into splenic T cells. At 10 mg / kg, the incorporation index reached a peak, three times higher than the control group. However, LBP at 25 mg / kg and 50 mg / kg significantly inhibited splenic T cell transformation, indicating a dose-dependent effect of LBP on splenic T lymphocyte proliferation. The effect of LBP on 3H-TdR incorporation into cells induced by the B lymphocyte mitogen LPS was observed at the dose of 10 mg / kg / day, which showed the most significant increase in splenic T lymphocyte transformation. At LPS concentrations of 50.70 and 100 μg / ml, LBP had no significant effect on the 3H-TdR incorporation index in B cells, indicating that LBP is insensitive to B cell mitogen-induced cell transformation.

[0004] 1.1.2. Effect on Antibody-Forming Cells in the Spleen: LBP significantly increased the number of spleen PFCs at a dose of 5.20 mg / kg / day for 7 days, with the 5 mg / kg dose increasing the number to 1.4 times that of the normal immune control group. However, at a dose of 25.50 mg / kg, the number of PFCs was significantly reduced, with the 25 mg / kg group having a PFC number of 43% of the normal control group. This indicates that LBP has an appropriate dose range for enhancing the number of antibody-forming cells.

[0005] 1.1.3. Effect on spleen Ts cell function: The Ts cells in the spleen cells of the donor mice were observed to inhibit the PFC number of the normal immune mice by 63% using the SOI method, indicating that the SOI method can induce Ts cells that have a significant inhibitory effect on the antibody-producing cells. After the donor mice were given LBP 5 mg / (kg.day) for 7 days, the PFC number of the recipient mice was significantly decreased compared with the SOI control group, indicating that this dose has an enhancing effect on the function of Ts cells. When the dose was increased to 25 and 50 mg / kg, the enhancing effect of LBP on the function of Ts cells was significantly decreased.

[0006] 1.2. Effect of LBP on the cellular immune function of S180 tumor-bearing mice and its tumor inhibition: 1.2.1. Effect of LBP on the cellular immune function of normal mice: The mice were intraperitoneally injected with LBP 5 mg / (kg.day) for 7 days (do-6), and the spleen was taken on the 7th day to measure the T lymphocyte proliferation response. The results showed that LBP can significantly increase the ConA-induced T lymphocyte proliferation response of the spleen of normal mice, and the cpm value increased from 28410±3110 of the control group to 64870±2571 of the administration group.

[0007] 1.2.2. Effect of LBP on the cellular immune function of S180 tumor-bearing mice and its tumor inhibition: Two days after the mice were subcutaneously inoculated with S180 tumor cells, they were intraperitoneally injected with LBP 10 mg or 20 mg / (kg.day) for 7 days, and the mice were sacrificed on the 9th day to measure the tumor weight and T lymphocyte proliferation response. The results showed that the tumor inhibition rates of LBP 10 mg and 20 mg / kg were 31% and 39%, respectively. At the same time, the T lymphocyte proliferation response cpm value of the mice in the LBP 10 mg group increased from 139±62 of the non-administration group to 5581±783 of the administration group, and the RPI (Relative proliferation index) increased from 0.3% of the normal to 24.6%. Cyclophosphamide (Cy) was used as a positive control, and the tumor inhibition rate was 47% after a single subcutaneous injection of 25 mg / kg, and the T lymphocyte proliferation response cpm value was 165±31.

[0008] 1.2.3. Effect of LBP on the cellular immune function of normal mice inhibited by cyclophosphamide: The mice were intraperitoneally injected with LBP 5 mg / (kg.day) for 7 days on the same day of subcutaneous injection of Cy 25 mg / kg, and the spleen was taken on the 7th day to measure the T cell proliferation response. The RPI was 105% after the addition of LBP (the RPI of the mice in the Cy alone group was 33% of the normal), indicating that LBP completely counteracted the effect of Cy on the low cellular immune function.

[0009] 1.2.4. Antitumor Effect of Lycium Barbarum Polysaccharide Combined with Cyclophosphamide: Two days after subcutaneous inoculation of S180 cells, mice were injected subcutaneously with 25 mg / kg of Cy, resulting in a 31% tumor inhibition rate. Simultaneous intraperitoneal injection of LBP (10 mg / kg / day) for 7 days increased the inhibition rate to 47%, although this difference was not significant (P>0.05). When Cy was administered subcutaneously at 12.5 mg / kg, the inhibition rate was 14%, with no significant difference in tumor weight compared with the control group. However, when LBP (10 mg / kg / day) was administered intraperitoneally for 7 days, the inhibition rate increased to 54% (P<0.01), indicating that LBP enhances the antitumor effect of Cy.

[0010] 1.3. Effects of Lycium barbarum polysaccharide and combined administration of anaerobic Corynebacterium parvum vaccine on the anti-tumor activity of mouse peritoneal macrophages: Intraperitoneal injection of Lycium barbarum polysaccharide (40 mg / kg / day) for 7 days in normal mice enhanced the anti-tumor activity of ConA-treated macrophages in tumor target cell proliferation. LBP (5, 10, 20, or 40 mg / kg / day) combined with a low-dose (250 μg / mouse) anaerobic Corynebacterium parvum vaccine (CP) exhibited a significant synergistic effect. The effect was most pronounced at a concentration of 20 mg / kg of LBP, with inhibition rates of 85.5% and 63.6% on target cell proliferation, P815, and P388, respectively, compared to 28.1% and 24.0% in the CP control group. This suggests that combined administration of LBP and CP can reduce the dosage of both, enhance their efficacy, and reduce the toxic side effects of CP. LBP is an immunopotentiator that primarily acts on T cells, enhancing the function of CTLs and NK cells; CP is a macrophage stimulator. Experiments have shown that the combined use of immunopotentiators that act on different aspects of the immune response can produce a synergistic effect. Experiments have also demonstrated that LBP activates macrophages in both nonspecific and specific anti-tumor responses.

[0011] 1.4. Lycium barbarum polysaccharides enhance interleukin-2 activity in mice: Adding LBP to the culture medium (5×10 cells / ml and 4 μg / ml ConA) for inducing interleukin-2 (IL-2) production in mouse splenocytes resulted in an IL-2-containing supernatant that increased the in vitro proliferation activity of adult mouse thymocytes (using the [3H]TdR incorporation assay). The level of IL-2-induced lymphocyte proliferation in aged mice was significantly lower than that in adults. LBP significantly enhanced this IL-2 effect in aged mice, reaching adult levels. This experiment demonstrated that LBP enhanced IL-2 activity and restored IL-2 activity in aged mice.

[0012] 1.5. Modulatory Effects of Lycium Barbarum Polysaccharide on Immune Function in Aged Mice: The number of splenic plaque-forming cells (PFCs) in aged mice was significantly lower than that in normal adult mice, decreasing by 51%. Administration of LBP (20 mg / kg / day) intraperitoneally for 7 days significantly increased the number of PFCs in the spleen of aged mice to normal adult levels. ConA-induced 3H-TdR incorporation into thymocytes in aged mice was also significantly reduced. LBP administration significantly increased this incorporation, with 5 mg / kg of LBP significantly reducing it. LBP administration significantly increased this incorporation, with 5 mg / kg of LBP increasing it by approximately 10-fold. Superoptimal SRBC immunization (SOI) assays demonstrated that an appropriate dose of LBP significantly modulated suppressor T cells (Ts) in aged mice, enhancing Ts cell activity.

[0013] 1.6. Immunopharmacological Effects of Lycium Barbarum Polysaccharide on T, Cytotoxic T, and NK Cells in Mice and Its Counteracting the Immunosuppressive Effects of Cyclophosphamide: Intraperitoneal administration of 5-10 mg / kg of LBP increased the proliferation of splenic T lymphocytes in mice and enhanced the cytotoxic T lymphocyte (CTL) cytotoxicity, raising the specific cytotoxicity rate from 33% to 67%. Intraperitoneal administration of 5 mg / kg of LBP enhanced the cytotoxicity of NK cells, increasing the cytotoxicity rate from 12.4% to 18%. 5-10 mg / kg of LBP counteracted the immunosuppressive effects of cyclophosphamide (Cy) on T, CTL, and NK cells in mice. The relative proliferation index (RPI) of T lymphocytes increased from 33% to 105%, the CTL inhibition rate of Cy decreased from 51% with Cy alone to 19% and 36% with LBP in combination, and the NK cell cytotoxicity rate increased from 9.5% with Cy alone to 15% and 16% with Cy. These results indicate that LBP enhances T cell-mediated immune responses and NK cell activity in both normal and Cy-treated mice. Furthermore, LBP significantly increases the number and activity of macrophage C3b and Fc receptors and mitigates the inhibitory effects of cortisone acetate. Gavage administration of 2 ml of LBP tea bags per rat daily for two weeks significantly increased immunoglobulin levels (especially IgM) and complement activity.

[0014] 2. Hypoglycemic effect: Intraperitoneal injection of 6g / kg of wolfberry extract can reduce rabbit blood sugar by about 13% within 2-3 hours, and then gradually recover. Some people believe that its hypoglycemic effect is due to the guanidine derivatives it contains.

[0015] 3. Anti-fatty liver effect: Long-term (75 days) oral administration of betaine from the fruit increased phospholipid levels in the blood and liver of rats. Taking betaine before or simultaneously with carbon tetrachloride administration counteracted the decrease in phospholipid and total cholesterol levels in the rat liver, and also improved levels of BSP, SGP-T, alkaline phosphatase, and cholinesterase. This is primarily due to betaine's role as a methyl donor in the body.

[0016] 4. Effect on blood pressure: Intravenous injection of 20 mg / kg of the fruit's water-soluble extract can lower the blood pressure of anesthetized rabbits and stimulate their breathing; injection of atropine and severing of the vagus nerves on both sides can eliminate its antihypertensive effect; it has an inhibitory effect on the isolated rabbit heart and causes vasoconstriction in the rabbit ears. Extracts from organic solvents such as methanol and acetone also have a slight antihypertensive effect.

[0017] 5. Wolfberry extract can promote the growth and gas production of Lactobacillus casei and Lactobacillus acidophilus.

[0018] 6. Effect of Lycium Barbarum Decoction on Hydroxyproline Content, Hypoxia Tolerance, and Anti-fatigue Effects in Mice: Hydroxyproline is an amino acid found in collagen fibers and proteins, derived from the hydroxylation of proline. During aging, insufficient oxygen supply impairs the hydroxylation of proline, resulting in low hydroxyproline content in collagen, shrinkage and deformation of major organs, and weight loss in the elderly. This impact on lung function leads to decreased vital capacity, reduced reserve capacity, decreased resistance, decreased muscle strength, and decreased tolerance to hypoxia and fatigue. Therefore, hydroxyproline content is associated with aging. Mice were given a 13.23% Lycium Barbarum Decoction (0.3 ml / 20 g) orally once daily. After 30 days, hydroxyproline concentration increased by 15.49% compared to controls. When 0.3 ml / 20 g of wolfberry decoction was injected intraperitoneally into mice, their hypoxia tolerance was improved to 37.5 ± 2.5 minutes, compared to the control group's survival time of 27.9 ± 1.3 minutes (P < 0.01). The anti-fatigue test also showed a significant difference compared to the control group (P < 0.01).

[0019] 7. Anti-tumor effect: In vitro tests showed that wolfberry fruits and leaves have significant inhibitory effects on human gastric adenocarcinoma KATo-Ⅲ cells, and wolfberry stalks and leaves have significant inhibitory effects on human cervical cancer Hela cells. The mechanism of action is mainly manifested in inhibiting cell DNA synthesis, interfering with cell division, and reducing cell repopulation ability.

[0020] Selenium tea contains organic selenium, an essential trace element for the human body that participates in many important physiological functions. Numerous studies have shown that the occurrence of many diseases is closely related to a severe deficiency in selenium intake. The high incidence of endemic diseases such as Keshan disease and Kashin-Beck disease is closely related to selenium deficiency. High rates of malignant tumors such as liver and stomach cancer have also been found in selenium-deficient areas.

[0021] Selenium-Goji Liver-Tonifying Mixture uses an optimized extraction process to maximize the effective ingredients of goji berries and selenium-containing tea. It is used to treat liver and kidney yin deficiency in cancer patients after chemotherapy and radiotherapy, with symptoms such as dizziness, dry mouth and throat, soreness of the waist and knees, poor sleep, and tinnitus.

[0022] Chinese patent publication number CN1250119C discloses a golden goji-selenium tea and its production process. The process involves extracting beneficial ingredients through spring water soaking and filtration, but the extraction efficiency is low, resulting in significant waste of raw materials. Therefore, a production process for a goji-selenium tea mixture is proposed. Summary of the Invention

[0023] In view of the shortcomings of the existing technology, the present invention provides a preparation process of selenium-qi liver-tonifying mixture, which solves the technical problem of not being able to maximize the extraction of effective ingredients from medicinal materials.

[0024] To achieve the above objectives, the present invention provides the following technical solutions.

[0025] A preparation process of a selenium-qi liver-tonifying mixture is characterized by comprising the following steps: S1: Decoction of wolfberries: Weigh wolfberries and water according to a mass ratio, boil the wolfberries in water in an extraction tank, and filter to form a first decoction liquid; add water to the extraction tank a second time, boil the wolfberries first, and then add tea leaves, decoct the tea leaves and the wolfberry decoction together to fully blend the effective ingredients of the wolfberries with the water, and filter to form a second decoction liquid; The first decoction weight ratio is: wolfberry 55-65kg; water 450kg-500kg; The weight ratio of water and tea leaves added in the second decoction is 450kg-500kg of water and 55-80kg of tea leaves. S2 mixes the first decoction liquid and the second decoction liquid to form a decoction liquid; S3: letting the decoction in step S2 stand and then filtering to form a filtrate; S4: concentrating the filtrate in step S3 under reduced pressure to form a concentrate; S5: adding ethanol to precipitate the concentrated solution in step S4, letting it stand and then filtering to form a filtrate; S6: decompressing and concentrating the filtrate in step S5 until there is no alcohol smell, thereby forming an ointment; S7 adds honey, royal jelly, purified water and ointment, mixes and stirs evenly to form a prepared liquid, cools and settles the prepared liquid, filters to form a medicinal liquid; stirs the medicinal liquid evenly, cools and settles, filters to form a filling medicinal liquid; Honey and royal jelly mass ratio, Honey 3-5kg; Royal jelly 2-4kg; 180-220 liters of purified water.

[0026] The production process of the selenium-wolfberry liver-tonifying mixture as described above is characterized in that the tea leaves in step S1 are selenium-enriched tea.

[0027] The production process of the selenium-wolfberry liver-tonifying mixture as described above is characterized in that: in the step S1, wolfberries are weighed according to the mass ratio and decocted with water, the first decocted water is filtered and extracted, and the first decocted water is placed in another tank for storage; then water is added to the extraction tank again and decocted with wolfberries, and then tea leaves are added and decocted together, and the second decocted water is filtered and extracted.

[0028] The production process of the selenium-qi liver-tonifying mixture as described above is characterized in that: in the step S6, the filtrate is concentrated under reduced pressure until there is no alcohol taste.

[0029] The production process of the selenium-wolfberry liver-tonifying mixture as described above is characterized in that: in the step S7, the prepared liquid is frozen and stored, and filtered through a high-speed tubular centrifuge, and the precipitate is discarded to form a medicinal liquid; the medicinal liquid is centrifuged and filtered through a high-speed tubular centrifuge, and the precipitate is discarded to form a filling medicinal liquid.

[0030] The manufacturing process of the selenium-qi liver-tonifying mixture as described above is characterized in that: in the step S7, the filled medicinal liquid is pumped into a storage tank for inspection and standby use.

[0031] Subsequently, the bottles are cleaned and dried, sealed, sterilized, inspected by light, and packaged to produce the bottled mixture. The final mixture should contain 8.0-12.0 μg of selenium (Se) per ml.

[0032] The present invention requires the following main equipment: a multi-functional extraction tank, a double-effect concentrator, a precipitation tank, a liquid preparation tank, a high-speed tubular centrifuge, etc., all of which are existing technology products.

[0033] Compared with the existing technology, the technical solution of the present application has the following beneficial effects: the process of the present invention first extracts the beneficial ingredient organic selenium in wolfberry and selenium-containing tea into the medicinal liquid through decoction, and the medicinal liquid after filtering the medicinal residue is first concentrated under reduced pressure, then precipitated with alcohol, and then concentrated under reduced pressure to remove alcohol, thereby increasing the drug concentration, reducing the decomposition and loss of heat-sensitive components, and maintaining the efficacy. The above process can ensure the maximum extraction of the effective ingredients of the medicinal materials, and has the characteristics of good taste and high selenium content. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 It is a process flow chart of the present invention. DETAILED DESCRIPTION

[0035] The technical solutions in the embodiments of the present invention will be described clearly and completely below with reference to the accompanying drawings in the embodiments of the present invention.

[0036] See also Figure 1 A preparation process of a selenium-qi liver-tonifying mixture comprises the following steps: S1: Decoction of wolfberries: Weigh wolfberries according to their mass ratio, add water, and decoct for 0.8-1.2 hours. Filter and extract the first decoction liquid, and store the first decoction liquid in another tank; then add water to the extraction tank and decoct the wolfberries again for 0.3-0.4 hours to fully blend the active ingredients of the wolfberries with the water; then add tea leaves and decoct together for 10-15 minutes. The tea leaves are selenium-containing tea, and the second decoction liquid is filtered and extracted; The first decoction weight ratio is: wolfberry 55-65kg; water 450kg-500kg; The weight ratio of water and tea leaves added in the second decoction is 450kg-500kg of water and 55-80kg of tea leaves. Preferably, the first decoction is performed with 55 kg of wolfberry and 500 kg of water; the second decoction is performed with the water and tea leaves having a mass ratio of 450 kg of water and 80 kg of tea leaves.

[0037] S2; mixing the first decoction liquid and the second decoction liquid together to form a decoction liquid; S3: standing the decoction in step S2 for 3-5 hours and filtering to form a filtrate; S4: The filtrate in step S3 is concentrated under reduced pressure by a double-effect concentrator at 60°C to a concentrate with a relative density of 1.20-1.25 (measured at 20°C); S5 adds ethanol to make the concentrated solution contain 66-69% alcohol, preferably 66% alcohol, and filter to form a filtrate; S6: the filtrate in step S5 is concentrated under reduced pressure at 60° C. until there is no alcohol smell, thereby forming an ointment; S7 adds honey, royal jelly, purified water and ointment, mixes and stirs evenly to form a prepared liquid, freezes and stores the prepared liquid, and filters it through a high-speed tubular centrifuge, discards the sediment, and forms a medicinal liquid; the medicinal liquid is stirred evenly, cooled and precipitated, and centrifuged and filtered through a high-speed tubular centrifuge, discards the sediment, and forms a filled medicinal liquid, which is pumped into a storage tank for inspection and standby use.

[0038] In step S7, the mass ratio of honey to royal jelly is: 3-5 kg ​​of honey; 2-4 kg of royal jelly; and 180-220 liters of purified water.

[0039] Preferred are 3kg honey, 2kg royal jelly, and 180 liters of purified water.

[0040] Bottles are sorted, washed, dried, filled, sterilized, inspected, and packaged to create the bottled mixture. The process is as follows: First, unqualified oral C-type brown bottles are removed, then stacked upside down on a rotating tray for cleaning and placed in an oven for drying. The aluminum-plastic combination caps are rinsed three times with purified water, drained, and then disinfected by soaking in 75% ethanol for one hour. After drying, filling and capping begin. Cans that pass the drying process are placed in a sterilizer and sterilized at 100°C. Intermediate products that fail clarity or capping are removed from the inspection box. The surface is then wiped with 75% ethanol to remove any contaminants before being delivered for packaging. The final mixture should contain 8.0-12.0 μg of selenium (Se) per ml.

[0041] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A preparation process of a selenium-qi liver-tonifying mixture, characterized by comprising the following steps: S1: Decoction of wolfberries: Weigh wolfberries and water according to a mass ratio, boil the wolfberries in water in an extraction tank, and filter to form a first decoction liquid; add water to the extraction tank a second time, boil the wolfberries first, and then add tea leaves, decoct the tea leaves and the wolfberry decoction together to fully blend the effective ingredients of the wolfberries with the water, and filter to form a second decoction liquid; The first decoction weight ratio is: wolfberry 55-65kg; water 450kg-500kg; The weight ratio of water and tea leaves added in the second decoction is 450kg-500kg of water and 55-80kg of tea leaves. S2 mixes the first decoction liquid and the second decoction liquid to form a decoction liquid; S3: letting the decoction in step S2 stand and then filtering to form a filtrate; S4: concentrating the filtrate in step S3 under reduced pressure to form a concentrate; S5: adding ethanol to precipitate the concentrated solution in step S4, letting it stand and then filtering to form a filtrate; S6: decompressing and concentrating the filtrate in step S5 until there is no alcohol smell, thereby forming an ointment; S7 adds honey, royal jelly, purified water and ointment, mixes and stirs evenly to form a prepared liquid, cools and settles the prepared liquid, filters to form a medicinal liquid; stirs the medicinal liquid evenly, cools and settles, filters to form a filling medicinal liquid; Honey and royal jelly mass ratio, Honey 3-5kg; Royal jelly 2-4kg; 180-220 liters of purified water.

2. The preparation process of the selenium-qi liver-tonifying mixture according to claim 1, characterized in that: The tea leaves in step S1 are selenium-enriched tea.

3. The preparation process of the selenium-qi liver-tonifying mixture according to claim 1, characterized in that: In step S1, wolfberries are weighed according to a mass ratio, water is added and decocted, the first decoction liquid is filtered and extracted, and the first decoction liquid is stored in another tank; then water is added to the extraction tank again and decocted with wolfberries, and then tea leaves are added and decocted together, and the second decoction liquid is filtered and extracted.

4. The preparation process of the selenium-qi liver-tonifying mixture according to claim 1, characterized in that: In step S6, the filtrate is concentrated under reduced pressure until the alcohol taste is eliminated.

5. The preparation process of the selenium-qi liver-tonifying mixture according to claim 1, characterized in that: In step S7, the prepared solution is frozen and stored, and filtered through a high-speed tubular centrifuge to discard the precipitate to form a medicinal solution; The drug solution is centrifuged and filtered through a high-speed tubular centrifuge, and the precipitate is discarded to form a filling drug solution.

6. The preparation process of the selenium-qi liver-tonifying mixture according to claim 5, characterized in that: In step S7, the filled liquid medicine is pumped into a storage tank for inspection and standby use.

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