Multi-omics screening cultivation equipment based on compound mutation of forage grass

Abstract

The invention relates to the technical field of forage grass cultivation, and discloses a multi-omics screening cultivation device based on forage grass compound mutagenesis, the multi-omics screening cultivation device comprises a first frame body, a second frame body and a mechanical arm, the inner side of the second frame body is detachably connected with a plurality of cultivation plates, and one side of the top end of the second frame body is fixedly connected with a quantitative box; a mounting shell is fixedly connected to one side of the top of the quantifying box, a driving motor is mounted on the outer side of the quantifying box, a rotating disc is fixedly connected to the output end of the driving motor, and a fixing block is fixedly connected to the eccentric position of the outer portion of the mounting shell. By means of the innovative accurate nutrient solution spraying device, quantitative spraying of the nutrient solution is achieved according to the growth requirements of pasture. The device can accurately regulate and control the spraying amount and range, and it is ensured that each pasture can obtain appropriate nutrition. Excessive waste of nutrition is avoided, the potential damage risk of excessive nutrient solution to pasture is reduced, healthy growth of the pasture is assisted, and the breeding success rate and the planting quality of the pasture are improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of forage cultivation, in particular to a multi-omics screening and cultivation device based on forage compound mutagenesis. BACKGROUND

[0002] Forage cultivation is crucial for the development of animal husbandry. With the increasing demand and technological development, its importance is increasingly prominent. Traditional forage cultivation mainly relies on natural selection and hybrid breeding, which is low in efficiency and long in cycle. In recent decades, techniques such as tissue culture, genetic engineering and molecular marker-assisted breeding have been applied, which have improved the efficiency and accuracy of cultivation to some extent. However, the existing technology still has many shortcomings:

[0003] First, the nutrient supply is unreasonable. Traditional methods of nutrient liquid supply such as sprinkling irrigation and drip irrigation cannot accurately control the spraying amount and range according to the type of forage, the growth stage and environmental changes, which easily leads to waste of nutrients or excessive damage to forage.

[0004] Second, the screening efficiency is low. The existing screening methods mostly rely on manual observation and simple measurement, which cannot efficiently and accurately screen out samples meeting specific trait standards from numerous forage samples, and are prone to errors, high labor intensity and high cost.

[0005] Third, there is a lack of multi-omics integrated analysis. In forage mutagenic breeding, only single-omics technology cannot comprehensively analyze the growth and development rules of forage and the mutagenic mechanism, making it difficult to efficiently screen and cultivate excellent varieties.

[0006] Therefore, the technical personnel in the field propose a multi-omics screening and cultivation device based on forage compound mutagenesis to solve the above problems. SUMMARY

[0007] In view of the shortcomings of the prior art, the present application provides a multi-omics screening and cultivation device based on forage compound mutagenesis, which solves the problems raised in the background art.

[0008] In order to achieve the above object, the present application is realized by the following technical scheme: the multi-omics screening and cultivating equipment based on forage grass composite mutagenesis, comprising a first frame, a second frame and a mechanical arm, a plurality of cultivation plates are detachably connected to the inner side of the second frame, a quantitative box is fixedly connected to the top side of the second frame, a mounting shell is fixedly connected to the top side of the quantitative box, a drive motor is installed on the outer side of the quantitative box, the output end of the drive motor is fixedly connected with a rotating disc, the eccentric part of the outer part of the rotating disc is fixedly connected with a fixed block, the outer surface of the rotating disc is slidably connected with a movable frame, the outer side of the movable frame is fixedly connected with a connecting shaft, one end of the connecting shaft is fixedly connected with a rubber piston, the inside of the mounting shell is elastically connected with a shielding block through a tension spring, two through grooves are formed in the lower surface of the mounting shell, the outer surface of the quantitative box is communicated with a mounting pipe, the outer surface of the mounting pipe is communicated with a plurality of liquid spraying pipes two, the mechanical arm screens out the forage grass samples with successful mutagenesis through an intelligent screening system.

[0009] Preferably, the top left side of the second frame is fixedly connected with a storage box two, the outer side of the storage box two is installed with a pump body two, the output end of the pump body two is fixedly connected with a second communication pipe, the input end of the pump body two is fixedly connected with a first communication pipe, and a plurality of placement plates are installed on the inner side of the first frame.

[0010] Preferably, one end of the second communication pipe is communicated with the top of the mounting shell, and one end of the first communication pipe is communicated with the outer side of the storage box two.

[0011] Preferably, the inside of the movable frame is provided with a movable groove, and the fixed block is movably connected in the inside of the movable groove.

[0012] Preferably, the outer surface of each liquid spraying pipe two is installed with a control valve, and the top of the cultivation plate is installed with a flow meter.

[0013] Preferably, the top side of the first frame is fixedly connected with a storage box one, the outer side of the storage box one is installed with a pump body one, the output end of the pump body one is fixedly connected with a second connecting pipe, the input end of the pump body one is fixedly connected with a first connecting pipe, a servo motor is fixedly installed on the top middle side of the first frame, the output end of the servo motor is fixedly connected with a drive gear, the inner side of the first frame is slidably connected with two rack plates, the outer side of the rack plate is fixedly connected with a laser irradiation lamp, and the outer surface of the laser irradiation lamp is installed with a liquid spraying pipe one.

[0014] Preferably, the outer sides of the two rack plates are meshingly connected with the outer side of the drive gear, and one end of the second connecting pipe is communicated with the outer side of the liquid spraying pipe one through a shunt pipe.

[0015] Preferably, the longitudinal section of the rubber piston is square, and the outer surface of the rubber piston is in close contact with the inner wall of the dosing chamber.

[0016] Preferably, the intelligent screening system comprises:

[0017] An information acquisition module comprising an image acquisition device and a sensor, for acquiring images and growth data of the forage samples and transmitting the data to the data processing module;

[0018] A data processing module comprising a computer, a data storage unit, image recognition and analysis software, and a machine learning algorithm, for analyzing and processing the collected data, determining whether the forage samples meet the preset screening criteria, and transmitting the results to the control module;

[0019] A control module comprising a programmable logic controller and control software, for controlling the actions of the execution module according to the instructions of the data processing module;

[0020] An execution module for placing the forage samples that meet the criteria on the cultivation plate according to the instructions of the control module;

[0021] A communication network for connecting the above-mentioned modules.

[0022] The information acquisition module and the data processing module are connected through a data acquisition card, the data processing module and the control module are connected through an industrial Ethernet or a wireless communication module, the control module and the execution module are connected through an electrical interface, and the communication network covers the entire intelligent screening system.

[0023] The present application provides a multi-omics screening and cultivation device based on forage composite mutagenesis.

[0024] 1. The present application realizes quantitative spraying of nutrient solution according to the growth needs of forage through an innovative precise spraying device, which can accurately control the spraying amount and range.

[0025] 2. The present application utilizes the respective advantages of laser and chemical mutagens to increase the possibility and diversity of forage gene mutation, thereby improving the mutagenesis efficiency and success rate. Meanwhile, the introduction of driving gears and rack plates can make the laser irradiation and chemical mutagen solution more uniformly cover the forage samples, avoiding the problem of poor mutagenesis effect of some samples due to uneven mutagenesis.

[0026] 3、The intelligent screening system makes the device capable of accurately and efficiently screening out the forage grass samples meeting the standards, meanwhile, the image and growth data of the forage grass samples are collected by the image acquisition device and the sensor, and the data are comprehensively processed by the aid of the computer, the data storage unit and the image recognition and analysis software, so that the automatic identification and classification of the forage grass samples are realized, the error and labor intensity of manual screening are effectively reduced, and the labor cost is reduced. BRIEF DESCRIPTION OF DRAWINGS

[0027] Figure 1 is a perspective view of the present application;

[0028] Figure 2 is a first frame structure schematic view of the present application;

[0029] Figure 3 is a quantitative tank profile view of the present application;

[0030] Figure 4 is a storage tank structure schematic view of the present application;

[0031] Figure 5 is Figure 1 is an enlarged view of A in the middle;

[0032] Figure 6 is Figure 2 is an enlarged view of B in the middle;

[0033] Figure 7 is Figure 3 is an enlarged view of C in the middle;

[0034] Figure 8 is a mounting pipe structure schematic view of the present application;

[0035] Figure 9 is a flow chart of the intelligent screening system of the present application.

[0036] 1, first frame; 2, second frame; 3, mechanical arm; 401, storage tank one; 402, pump body one; 403, first connecting pipe; 404, servo motor; 405, laser irradiation lamp; 406, liquid spray pipe one; 407, rack plate; 408, driving gear; 409, second connecting pipe; 5, placing plate; 601, storage tank two; 602, first communication pipe; 603, pump body two; 604, second communication pipe; 701, quantitative tank; 702, mounting shell; 703, fixed block; 704, movable frame; 705, connecting shaft; 706, rubber piston; 707, shielding block; 708, mounting pipe; 709, tension spring; 710, through slot; 711, liquid spray pipe two; 712, control valve; 713, rotating disc; 8, cultivation plate. DETAILED DESCRIPTION

[0037] With reference to the drawings of the present application, the technical solutions in the embodiments of the present application will be described clearly and completely. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work fall within the scope of the present application.

[0038] Please refer to the drawings of the present application Figure 1 - the drawings of the present application Figure 8 The embodiment of the present application provides a multi-omics screening and breeding device based on forage grass compound mutagenesis, which comprises a first frame 1, a second frame 2 and a mechanical arm 3. The inner side of the second frame 2 is detachably connected with a plurality of breeding plates 8. The top side of the second frame 2 is fixedly connected with a quantitative box 701. The top side of the quantitative box 701 is fixedly connected with a mounting shell 702. A drive motor is installed on the outer side of the quantitative box 701. The output end of the drive motor is fixedly connected with a rotating disc 713. The eccentric part of the outer part of the rotating disc 713 is fixedly connected with a fixed block 703. The outer surface of the rotating disc 713 is slidingly connected with a movable frame 704. The outer side of the movable frame 704 is fixedly connected with a connecting shaft 705. One end of the connecting shaft 705 is fixedly connected with a rubber piston 706. The inside of the mounting shell 702 is elastically connected with a shielding block 707 through a tension spring 709. Two through grooves 710 are formed on the lower surface of the mounting shell 702. The outer surface of the quantitative box 701 is communicated with a mounting pipe 708. The outer surface of the mounting pipe 708 is communicated with a plurality of liquid spraying pipes two 711. The mechanical arm 3 screens out the forage grass samples with successful mutagenesis through an intelligent screening system. The inside of the movable frame 704 is provided with a movable groove. The fixed block 703 is movably connected in the inside of the movable groove. The outer surface of each liquid spraying pipe two 711 is provided with a control valve 712. The top of the breeding plate 8 is provided with a flow meter. The longitudinal section of the rubber piston 706 is square. The outer surface of the rubber piston 706 is fitted with the inner wall of the quantitative box 701.

[0039] Specifically, the first frame 1 and the second frame 2 constitute the overall frame of the device, supporting various components. The breeding plate 8 is used to place the forage grass samples, and the breeding plate 8 is detachably connected, which is convenient for replacement and maintenance. The mechanical arm 3 is connected with the intelligent screening system, which is used to screen out the forage grass samples with successful mutagenesis and place them on the breeding plate 8. The quantitative box 701 is used to store and quantitatively deliver nutrient solution. The quantitative box 701 is made of transparent material and provided with a scale on the outer surface, which is convenient for observation and control of the amount of nutrient solution. The mounting shell 702 stores nutrient solution inside. The nutrient solution enters the cavity on the inside of the quantitative box 701 through the through groove 710. The drive motor drives the rotating disc 713 to rotate through the output end, providing power for the delivery of nutrient solution.

[0040] The fixed block 703 is movably connected inside the movable slot. The outer side of the movable frame 704 is fixedly connected with the connecting shaft 705, which is driven to move synchronously with the left and right reciprocating movement of the movable frame 704. The outer surface of the rubber piston 706 is in close contact with the inner wall of the quantitative tank 701, ensuring that it can closely fit the inner wall of the quantitative tank 701 during movement, preventing the nutrient solution from leaking. The installation pipe 708 connects the quantitative tank 701 and the second liquid spraying pipe 711, which is used to deliver the nutrient solution to the planting groove inside the cultivation plate 8. The outer surface of each second liquid spraying pipe 711 is equipped with a control valve 712 for controlling the discharge of the nutrient solution. The flow meter is used to monitor the flow of the nutrient solution, ensuring that each plant receives appropriate nutrition.

[0041] When it is necessary to add nutrient solution to the cultivation plate 8, first open the installation valve on the outside of the installation pipe 708, so that the nutrient solution inside the installation shell 702 enters the cavity inside the quantitative tank 701 through the left through slot 710. When the cavity is filled with nutrient solution, start the drive motor on the outside of the quantitative tank 701. The drive motor drives the rotating disc 713 to rotate, and the fixed block 703 moves eccentrically along the outer surface of the rotating disc 713. Since the fixed block 703 is movably arranged in the movable slot of the movable frame 704, the movable frame 704 is driven to move left and right reciprocally. The left and right reciprocating movement of the movable frame 704 drives the rubber piston 706 to move in the quantitative tank 701 through the connecting shaft 705. The movement of the rubber piston 706 causes the nutrient solution in the cavity of the quantitative tank 701 to be extruded, and is discharged through the installation pipe 708. The nutrient solution flows along the installation pipe 708 and is discharged into the planting groove inside the cultivation plate 8 through the second liquid spraying pipe 711 on the top.

[0042] When the top cultivation plate 8 is filled with nutrient solution, the top control valve 712 is closed, the second layer control valve 712 is opened, and the above steps are repeated to ensure that the nutrient solution of each cultivation plate 8 is added in turn. Since the length of the connecting shaft 705 is fixed, the initial position of the rubber piston 706 can be changed to achieve quantitative addition of the nutrient solution. The quantitative tank 701 is made of transparent material and has a scale on the outer surface, which facilitates observation of the amount of nutrient solution, thereby accurately controlling the amount of nutrient solution added each time. This precise nutrient solution spraying method ensures that each plant receives appropriate nutrition, avoiding excessive waste of nutrients and reducing the potential damage of excessive nutrient solution to the grass, which helps the healthy growth of the grass and improves the success rate of breeding and the quality of grass planting.

[0043] The top left side of the second frame body 2 is fixedly connected with a storage box two 601, the outer side of the storage box two 601 is provided with a pump body two 603, the output end of the pump body two 603 is fixedly connected with a second communication pipe 604, the input end of the pump body two 603 is fixedly connected with a first communication pipe 602, and the inner side of the first frame body 1 is provided with a plurality of placing plates 5. One end of the second communication pipe 604 is communicated with the top of the mounting shell 702, and one end of the first communication pipe 602 is communicated with the outer side of the storage box two 601.

[0044] Specifically, the storage box two 601 is used for storing nutrient solution for the cultivation of pasture. The pump body two 603 is responsible for providing power to extract the nutrient solution in the storage box two 601 and deliver it to the mounting shell 702.

[0045] When it is necessary to supply the pasture with nutrient solution, the pump body two 603 is started. The pump body two 603 extracts the nutrient solution from the storage box two 601 through the first communication pipe 602, and then delivers the nutrient solution to the mounting shell 702 through the second communication pipe 604. The nutrient solution inside the mounting shell 702 then flows into the dosing box 701 through the through slot 710, and the dosing box 701 precisely sprays the nutrient solution into the planting groove of the cultivation plate 8 according to the steps described previously through the cooperation of components such as the driving motor, the rotating disc 713, the movable frame 704, the connecting shaft 705 and the rubber piston 706. This process ensures the continuity from storage to precise spraying of the nutrient solution, providing stable nutritional support for the healthy growth of the pasture.

[0046] The top side of the first frame body 1 is fixedly connected with a storage box one 401, the outer side of the storage box one 401 is provided with a pump body one 402, the output end of the pump body one 402 is fixedly connected with a second connecting pipe 409, the input end of the pump body one 402 is fixedly connected with a first connecting pipe 403, the top middle side of the first frame body 1 is fixedly provided with a servo motor 404, the output end of the servo motor 404 is fixedly connected with a driving gear 408, the inner side of the first frame body 1 is slidably connected with two rack plates 407, the outer side of the rack plate 407 is fixedly connected with a laser irradiation lamp 405, and the outer surface of the laser irradiation lamp 405 is provided with a liquid spraying pipe one 406. The outer sides of the two rack plates 407 are in meshing connection with the outer side of the driving gear 408, and one end of the second connecting pipe 409 is communicated with the outer side of the liquid spraying pipe one 406 through a shunt pipe.

[0047] Specifically, when it is necessary to perform compound mutagenesis treatment on the pasture, the pump body one 402 is started. The pump body one 402 extracts the chemical mutagenic solution from the storage box one 401 through the first connecting pipe 403, and then delivers the chemical mutagenic solution to the shunt pipe through the second connecting pipe 409. The shunt pipe distributes the chemical mutagenic solution to the liquid spraying pipe one 406, and the liquid spraying pipe one 406 uniformly sprays the chemical mutagenic solution on the pasture sample through the spray head.

[0048] Meanwhile, the servo motor 404 is started, and the output end of the servo motor 404 drives the driving gear 408 to rotate. The driving gear 408 is engaged with the two rack plates 407, and drives the rack plates 407 to move along the inner side of the first frame body 1. The movement of the rack plates 407 drives the laser irradiation lamp 405 and the liquid spraying pipe 406 to move synchronously. By controlling the forward and reverse rotation of the output end of the servo motor 404, the reciprocating movement of the liquid spraying pipe 406 and the laser irradiation lamp 405 can be realized. In this way, the laser irradiation and the chemical mutagenesis solution can be more uniformly covered on the pasture sample, avoiding the problem of poor mutagenesis effect of part of the samples caused by uneven mutagenesis treatment.

[0049] Please refer to the accompanying Figure 9 The present application also provides an intelligent screening system, wherein the intelligent screening system comprises:

[0050] An information acquisition module comprising an image acquisition device and a sensor for acquiring images and growth data of the pasture sample and transmitting the data to the data processing module;

[0051] A data processing module comprising a computer, a data storage unit, image recognition and analysis software, and a machine learning algorithm for analyzing and processing the collected data, determining whether the pasture sample meets the preset screening criteria, and transmitting the results to the control module;

[0052] Data preprocessing is to clean and normalize the collected raw data, remove noise and outliers, and convert the data into a form suitable for analysis. For example, normalize the image data by scaling the pixel values to a specific range, and the formula is:

[0053]

[0054] where X norm is the normalized data, X is the original data, X min and X max are the minimum and maximum values of the original data, respectively.

[0055] where feature extraction is to extract key features from preprocessed data for subsequent analysis. For image data, a convolutional neural network (CNN) can be used to extract features. CNN automatically extracts image features through convolutional layers and pooling layers. The convolution operation formula is:

[0056] (I*K)(i,j)=∑ m ∑ m I(i+m,j+n)K(m,n);

[0057] where I: represents the input image, usually a two-dimensional matrix, containing the pixel values of the image.

[0058] K: represents a convolution kernel, which is also a two-dimensional matrix, containing weight values used to extract image features in the convolution operation. i: represents the row index of the output image; j: represents the column index of the output image. m: represents the offset of the convolution kernel in the row direction. n: represents the offset of the convolution kernel in the column direction. (I*K)(i,j) represents the pixel value of the output image at position (i,j) after the convolution operation.

[0059] A control module comprising a programmable logic controller and control software for controlling the actions of the execution module according to the instructions of the data processing module;

[0060] An execution module for placing standard-compliant grass samples on the cultivation plate 8 according to the instructions of the control module;

[0061] A communication network for connecting the above-mentioned modules.

[0062] Wherein the information collection module is connected with the data processing module through a data acquisition card, the data processing module is connected with the control module through an industrial Ethernet or a wireless communication module, the control module is connected with the execution module through an electrical interface, and the communication network covers the entire intelligent screening system.

[0063] Working principle: when using the device, the following operation steps are included:

[0064] The pump body one 402 transports the chemical mutagenic solution in the storage tank one 401 to the second connecting pipe 409 through the first connecting pipe 403, then to the liquid spraying pipe one 406 through the second connecting pipe 409 and the shunt pipe, and finally sprayed out through the nozzle, so that the chemical mutagenic solution is sprayed on the outer surface of the first frame body 1, and the laser irradiation lamp 405 is used to realize the mutagenesis of the grass gene. At this time, the servo motor 404 is started to drive the driving gear 408 to rotate, and the driving gear 408 drives the two rack plates 407 to move, and the laser irradiation lamp 405 and the liquid spraying pipe one 406 are synchronously moved at the same time. Only the forward and reverse rotation of the output end of the servo motor 404 is controlled, and then the liquid spraying pipe one 406 is controlled to rotate forward and backward, so that the laser irradiation lamp 405 and the liquid spraying pipe one 406 move back and forth along the top of the first frame body 1, and then the laser irradiation and the solution of the chemical mutagenic agent can be more uniformly covered on the grass sample, avoiding the problem of poor mutagenesis effect of part of the sample caused by uneven mutagenesis;

[0065] The intelligent screening system is used to screen and identify standard-compliant grass samples, and the standard-compliant grass samples are moved to the planting groove inside the cultivation plate 8 through the mechanical arm 3.

[0066] When the quantitative infusion of nutrient solution to the multi-layer cultivation plate 8 is needed, the nutrient solution in the storage tank two 601 is transported to the second communication pipe 604 through the first communication pipe 602 by driving the pump body two 603, and then to the inside of the mounting shell 702. At this time, the nutrient solution in the mounting shell 702 enters the cavity inside the quantitative tank 701 through the left through groove 710, and when the cavity is filled with nutrient solution, the mounting valve outside the mounting pipe 708 is opened, the drive motor outside the quantitative tank 701 is started, and the rotating disc 713 is rotated by the output end of the drive motor. At this time, the fixed block 703 moves eccentrically along the outer surface of the rotating disc 713, and since the fixed block 703 is movably arranged in the movable groove, the movable frame 704 moves left and right reciprocally, and the connecting shaft 705 moves synchronously at the same time. In this way, the rubber piston 706 moves along the direction of the mounting pipe 708 under the traction of the connecting shaft 705, and the nutrient solution in the cavity of the quantitative tank 701 is discharged through the mounting pipe 708. The mounting pipe 708 discharges into the planting groove inside the cultivation plate 8 through the top liquid injection pipe two 711, and when the top cultivation plate 8 is filled with nutrient solution, the top control valve 712 is closed and the second layer control valve 712 is opened. The above steps are repeated to ensure that the nutrient solution of each cultivation plate 8 is added. Since the length of the connecting shaft 705 is fixed, and the quantitative tank 701 is made of transparent material and has a scale on the outer surface, the initial position of the rubber piston 706 can be changed to realize the quantitative addition of nutrient solution. It ensures that each plant gains appropriate nutrition. It not only avoids excessive waste of nutrients, but also reduces the potential damage risk of excessive nutrient solution to the grass, helps the healthy growth of the grass, and improves the breeding success rate and the planting quality of the grass.

[0067] When the rubber piston 706 moves along the direction of the mounting pipe 708, the nutrient solution in the cavity enters the inside of the mounting shell 702 through the right through groove 710, and pushes the blocking block 707 to move left, until the upper surface of the blocking block 707 blocks the discharge port of the second communication pipe 604. When the nutrient solution in the quantitative tank 701 is discharged, the blocking block 707 is reset under the elastic action of the tension spring 709 to realize the next quantitative operation.

[0068] Although embodiments of the present application have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the application, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A multi-omics screening and cultivation device based on forage compound mutagenesis, comprising a first frame (1), a second frame (2) and a robotic arm (3), characterized in that: The inner side of the second frame (2) is detachably connected to a plurality of cultivation plates (8); the top side of the second frame (2) is fixedly connected to a quantitative box (701); the top side of the quantitative box (701) is fixedly connected to a mounting shell (702); a driving motor is installed on the outer side of the quantitative box (701); the output end of the driving motor is fixedly connected to a rotating disk (713); the outer eccentric portion of the rotating disk (713) is fixedly connected to a fixed block (703); the outer surface of the rotating disk (713) is slidably connected to a movable frame (704); the movable frame (704) is fixedly connected to the outer surface of the rotating disk (713); 04) is fixedly connected to the outside of the device, and one end of the connecting shaft (705) is fixedly connected to a rubber piston (706). The inside of the mounting shell (702) is elastically connected to a blocking block (707) via a tension spring (709). Two through grooves (710) are provided on the lower surface of the mounting shell (702). The outer surface of the quantitative box (701) is connected to a mounting pipe (708), and the outer surface of the mounting pipe (708) is connected to a plurality of spray pipes (711). The robotic arm (3) selects the successfully induced forage grass samples through the intelligent screening system.

2. The multi-omics screening and cultivation equipment based on forage compound mutagenesis according to claim 1 is characterized in that: A second storage box (601) is fixedly connected to the left side of the top of the second frame (2), a second pump body (603) is installed on the outside of the second storage box (601), the output end of the second pump body (603) is fixedly connected to the second connecting pipe (604), the input end of the second pump body (603) is fixedly connected to the first connecting pipe (602), and a plurality of placement plates (5) are installed on the inner side of the first frame (1).

3. The multi-omics screening and cultivation equipment based on forage compound mutagenesis according to claim 2 is characterized in that: One end of the second communicating tube (604) is connected to the top of the mounting shell (702), and one end of the first communicating tube (602) is connected to the outside of the second storage box (601).

4. The multi-omics screening and cultivation equipment based on forage compound mutagenesis according to claim 1 is characterized in that: A movable groove is provided inside the movable frame (704), and the fixed block (703) is movably connected inside the movable groove.

5. The multi-omics screening and cultivation equipment based on forage compound mutagenesis according to claim 1 is characterized in that: The outer surface of each of the second liquid spraying pipes (711) is provided with a control valve (712), and the top of the cultivation plate (8) is provided with a flow meter.

6. The multi-omics screening and cultivation equipment based on forage compound mutagenesis according to claim 1 is characterized in that: A storage box (401) is fixedly connected to one side of the top of the first frame (1), a pump body (402) is installed on the outside of the storage box (401), the output end of the pump body (402) is fixedly connected to the second connecting pipe (409), the input end of the pump body (402) is fixedly connected to the first connecting pipe (403), a servo motor (404) is fixedly installed on the middle side of the top of the first frame (1), the output end of the servo motor (404) is fixedly connected to the driving gear (408), the inner side of the first frame (1) is slidably connected to two rack plates (407), the outer side of the rack plate (407) is fixedly connected to a laser irradiation lamp (405), and the outer surface of the laser irradiation lamp (405) is installed with a liquid spraying pipe (406).

7. The multi-omics screening and cultivation equipment based on forage compound mutagenesis according to claim 6, characterized in that: The outer sides of the two rack plates (407) are meshed with the outer sides of the driving gear (408), and one end of the second connecting pipe (409) is connected to the outer side of the liquid spraying pipe (406) through a shunt pipe.

8. The multi-omics screening and cultivation equipment based on forage compound mutagenesis according to claim 1 is characterized in that: The longitudinal section of the rubber piston (706) is arranged to be square, and the outer surface of the rubber piston (706) is in contact with the inner wall of the quantitative box (701).

9. The multi-omics screening and cultivation equipment based on forage compound mutagenesis according to claim 1, characterized in that: The intelligent screening system comprises: An information acquisition module, which includes an image acquisition device and a sensor, is used to collect images and growth data of forage samples and transmit the data to a data processing module; The data processing module includes a computer, a data storage unit, image recognition and analysis software, and a machine learning algorithm, which is used to analyze and process the collected data, determine whether the forage sample meets the preset screening criteria, and transmit the results to the control module; The control module includes a programmable logic controller and control software, which is used to control the actions of the execution module according to the instructions of the data processing module; An execution module, configured to place a grass sample meeting the standards on a cultivation plate (8) according to an instruction of the control module; The communication network is used to connect the above modules.

10. The multi-omics screening and cultivation equipment based on forage compound mutagenesis according to claim 9, characterized in that: The information acquisition module is connected to the data processing module through a data acquisition card, the data processing module is connected to the control module through industrial Ethernet or a wireless communication module, the control module is connected to the execution module through an electrical interface, and the communication network covers the entire intelligent screening system.

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