Portulaca oleracea extract, cosmetic material composition and preparation method

Abstract

The invention provides a herba portulacae extract, a cosmetic material composition and a preparation method, and relates to the field of cosmetics. The purslane herb extract provided by the invention contains purslane herb plant cells. The cosmetic material composition contains the purslane herb extract. The portulaca oleracea extract and the cosmetic material composition contain portulaca oleracea plant cells and have the effects of resisting oxidation, resisting inflammation and relieving skin.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetics, in particular to a Portulaca oleracea extract, a cosmetic material composition and a preparation method. BACKGROUND

[0002] Human skin is composed of subcutaneous fat layer, dermis and epidermis, which has the function of regulating body temperature and playing the primary barrier role to the external environment. The subcutaneous fat layer is composed of adipocytes, the dermis is composed of matrix proteins such as collagen and elastic fibers, and the epidermis is composed of keratinocytes of stratified squamous epithelium. The dermis is located on the upper part of the subcutaneous fat layer, and the epidermis is located on the outside of the dermis. Human skin is constantly threatened by harmful environmental conditions such as ultraviolet rays, cold wind, dry weather, dust and electromagnetic waves. It is known that insufficient antioxidant capacity is the main cause of skin aging, and as the aging speed accelerates, consumers' demand for raw materials with excellent antioxidant capacity increases. In addition, due to the pollution of the earth's natural environment and the decline of human immunity, skin irritation and internal and external inflammation of the human body increase, so consumers' attention to raw materials with antioxidant, anti-inflammatory and soothing effects on the skin continues to increase.

[0003] The preparation of cosmetic raw materials derived from plants mainly uses organic solvents other than water to dissolve and extract active ingredients. Recently, various extraction methods have been tried to increase the extraction efficiency of effective ingredients contained in plants, but only the traditional extraction method of solvent extraction has been partially improved by changing physical extraction conditions such as temperature and pressure, and the mixing ratio of the extraction solvent. But this method has yet to improve the extraction efficiency of active ingredients in plants. SUMMARY

[0004] The present application aims to provide a Portulaca oleracea extract, a cosmetic material composition and a preparation method, which contains Portulaca oleracea plant cells and has antioxidant, anti-inflammatory and soothing effects on the skin.

[0005] In a first aspect, the present application provides a Portulaca oleracea extract containing Portulaca oleracea plant cells.

[0006] In a possible implementation, the Portulaca oleracea extract is obtained by treating Portulaca oleracea with an enzymatic method.

[0007] In a possible implementation, the mass fraction of the Portulaca oleracea plant cells in the Portulaca oleracea extract is 0.10wt%-90.00wt%, and can be 0.5wt%-10.00wt%.

[0008] In a second aspect, the present application provides the use of the Portulaca oleracea extract provided in the first aspect as a cosmetic raw material with antioxidant, anti-inflammatory and soothing effects on the skin.

[0009] In a third aspect, the present application provides a cosmetic material composition comprising the extract of Portulaca oleracea L. provided in the first aspect.

[0010] In a possible implementation manner, the cosmetic material composition is selected from any one of skin care water, gel, mask, foam, emulsion, cream, essence, sunscreen, foundation, primer, foundation cream, BB cream, CC cream, concealer, mascara, eyeliner, lip dye and lip gloss.

[0011] In a possible implementation manner, the mass ratio of the extract of Portulaca oleracea L. to the complex fermentation coenzyme is 8-12:1.

[0012] In a fourth aspect, the present application provides a preparation method of the extract of Portulaca oleracea L. provided in the first aspect, comprising the following steps: The pectin-degrading microorganism and the white rot fungus are respectively subjected to enrichment culture, and a pectinase fermentation coenzyme and a white rot fungus fermentation coenzyme are obtained, and the two coenzymes are mixed to form a complex fermentation coenzyme. The Portulaca oleracea L. powder and the complex fermentation coenzyme are subjected to enzymatic hydrolysis reaction, and the enzyme is inactivated to obtain the extract of Portulaca oleracea L.

[0013] In a possible implementation manner, the culture method of the pectinase fermentation coenzyme comprises inoculating an inoculum source containing a pectin-degrading microorganism group into a culture medium containing Portulaca oleracea L. powder, and sequentially performing 2 times of enrichment culture, and the method for each time of enrichment culture is 25-27°C, 50-100 rpm oscillation culture for 1.5-3 days to obtain the pectinase fermentation coenzyme. And / or, the culture method of the white rot fungus fermentation coenzyme comprises inoculating an inoculum source containing a white rot fungus into a culture medium containing Portulaca oleracea L. powder for culture, and the culture method is 25-27°C, 100-200 rpm oscillation culture for 10-12 days to obtain the white rot fungus fermentation coenzyme.

[0014] In a possible implementation manner, the mass ratio of the pectinase fermentation coenzyme to the white rot fungus fermentation coenzyme in the complex fermentation coenzyme is 4-6:4-6, and the mass ratio of the Portulaca oleracea L. powder to the complex fermentation coenzyme is 8-12:1. And / or, the method of the enzymatic hydrolysis reaction comprises first stirring at 28-32°C and 50-80 rpm for 1.5-2.5 hours, and then stirring at 23-27°C and 120-180 rpm for 1.5-2.5 hours.

[0015] Application effects The purslane extract and the cosmetic material composition containing the purslane extract of the present application contain purslane plant cells and can provide antioxidant, anti-inflammatory, and soothing effects to the skin. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 A preparation method of the purslane extract of Example 1 and Comparative Example 1 is shown.

[0017] Figure 2 A microscope image of the purslane extract of Example 1 is shown.

[0018] Figure 3 Evaluation results of the DPPH radical scavenging ability of the purslane extract of Example 1 and Comparative Example 1 are shown.

[0019] Figure 4 Evaluation results of the TNF-α expression inhibiting ability of the purslane extract of Example 1 and Comparative Example 1 are shown.

[0020] Figure 5 Evaluation results of the IL-2 expression inhibiting ability of the purslane extract of Example 1 and Comparative Example 1 are shown.

[0021] Figure 6 A graph showing the activity of pectinase coenzyme prepared from three different soil samples is shown.

[0022] Figure 7 A graph showing the activity of white rot fungus coenzyme during the preparation process is shown. DETAILED DESCRIPTION

[0023] Recently, in the food raw material industry, a plant single cell extraction method is being researched, which can reduce the increase in food storage, remove odors, stabilize effective components, color stabilization, regulate the digestion speed, thermal stability, and reduce environmental pollutants, etc. At the same time, the efficacy of inactivated lactic acid bacteria has been proven in the probiotic field, and at the same time, it is attracting attention as a health food together with a live lactic acid bacteria preparation. As is well known, inactivated lactic acid bacteria digested in the human digestive system as a component of the cell membrane, exhibit the same effects as live lactic acid bacteria in the intestine.

[0024] Generally, plant cell walls and cell membranes account for about 55% of plant cells and are composed of plant structural polysaccharides (e.g., cellulose, hemicellulose, and pectin) and storage sugars (e.g., starch). The biggest problem in isolating plant cells is that plant structural polysaccharides are present in primary and secondary cell walls and the middle lamella and are firmly bound to plant cell tissues. Due to this binding between plant cells, the extraction efficiency of active ingredients contained in the cell wall is low even with conventional organic solvents. Furthermore, lignin, which accounts for a large proportion of wood, negatively affects the extraction efficiency of plant active ingredients. In addition to using a method that improves extraction efficiency, when plant cells themselves are converted into active ingredients, a plant extract containing plant cells can provide a new direction for cosmetic raw materials if it exerts an effect on the skin in a manner similar to that of inactivated lactic acid bacteria.

[0025] The most likely fungus to be currently applicable to the biodegradation of lignin is white rot fungi, which secretes substrate-specific degradation enzymes, and has the ability to remove various recalcitrant chemicals and endocrine disruptors. White rot fungi can remove lignin from wood, and among lignin-degrading enzymes, there are manganese-dependent peroxidase (MnP), lignin peroxidase (LiP), and laccase.

[0026] As a conventional method for isolating plant cells, mechanical precision pulverization and protopectinase (PPase) treatment as a plant cell separation enzyme can be used. However, mechanical precision pulverization results in a low recovery rate of plant cells due to indiscriminate destruction of the adhesion tissues of plant cells. The PPase treatment method uses PPase obtained by culturing a single microorganism, and does not exhibit the same effect on the pectin structure of various plants in general because the types of pectin of various plants on which the enzyme acts are different. Since recalcitrant plant components (e.g., lignin) cannot be treated with PPase, a rapid and simple plant enzyme treatment method that can be applied to the pectin structure of various plants and other plant structural maintenance components is needed.

[0027] The cosmetic raw material of the present application comprises a plant cell of purslane and a purslane extract containing the same. Purslane (scientific name: Portulaca oleracea L.) is a whole plant of dried purslane belonging to the purslane family and is an annual herbaceous plant commonly found on the roadside. Purslane is well known as a plant with strong vitality that can survive for a long time even if it is uprooted, and has a shape resembling a horse's teeth. It is known that the main functional components of purslane include various components such as beta-carotene, alkaloids, coumarins, flavonoids, omega-3, tannins, saponins, vitamin A, cardiac glycosides, anthraquinone glycosides, etc. The pharmacological effects of these components include the prevention of arrhythmia, effects on colitis and diarrhea, diuretic effects, etc., and as a cosmetic raw material, they have effects such as skin whitening, moisturizing, soothing, irritation relief, anti-inflammatory effects, hair care, etc.

[0028] Generally, the initial response of inflammation starts from the introduction of immune cells into the tissue. Therefore, only by inhibiting the secretion of inflammatory mediators (NO, maleimide (PEG2), tumor necrosis factor alpha (TNF-alpha), interleukin-1 (IL-1), etc.), an anti-inflammatory effect can be expected, and the expression of inflammation production-related proteins such as cyclooxygenase 2 (COX-2) and nitric oxide synthase (iNOS) is also known as an important factor in regulating inflammation. As described above, the anti-inflammatory effect on the skin can be evaluated by measuring the expression of inflammatory mediators and inflammation production-related proteins.

[0029] Hereinafter, the embodiments of the present application will be described in detail so that those of ordinary skill in the art to which the present application pertains can easily practice the present application. The embodiments of the present application are provided in order to more completely explain the present application to those of ordinary skill in the art. Therefore, the embodiments of the present application can be modified in various other embodiments, and the scope of the present application is not limited to the embodiments described below.

[0030] Throughout the specification of the present application, when a certain part "comprises" a certain component, it means that other components are further included unless it is specifically stated to the contrary.

[0031] The terms "about", "substantially" and the like used to indicate degrees in the entire specification of the present application, when an inherent manufacturing and material allowable error is given in the meaning referred to, are used to indicate the meaning of the numerical value or close to the numerical value, and to prevent the disclosure of the meaning of referring to the accurate or absolute numerical value (which facilitates the understanding of the present application) from being unreasonably used by unscrupulous infringers. The term "step for" or "step of" used throughout the specification of the present application does not mean only "step for".

[0032] The present application provides a Portulaca oleracea extract, comprising Portulaca oleracea plant cells.

[0033] In the present application, the Portulaca oleracea plant cells refer to single cells.

[0034] In some embodiments of the present application, the Portulaca oleracea extract is obtained by treating Portulaca oleracea by enzymolysis. In the present application, Portulaca oleracea refers to its leaves, stems and other parts.

[0035] Enzymatic extraction is based on the composition of plant cell walls. According to the highly specific characteristics of enzyme reaction, the corresponding enzyme is selected to hydrolyze or degrade the components of cell walls (cellulose, hemicellulose and pectin), destroy the structure of cell walls, and make the components in the cells dissolved, suspended or colloidal in the solvent, so as to achieve the purpose of extraction and improve the extraction rate of components.

[0036] In some embodiments of the present application, the mass fraction of Portulaca oleracea plant cells in the Portulaca oleracea extract is 0.10wt%-90.00wt%, which can be selected as 0.50wt%-10.00wt%.

[0037] The present application also provides an application of the above-mentioned Portulaca oleracea extract, which is used as a cosmetic raw material with antioxidant, anti-inflammatory and soothing effects on the skin.

[0038] The present application also provides a cosmetic material composition comprising the above-mentioned Portulaca oleracea extract.

[0039] In some embodiments of the present application, the cosmetic material composition is selected from any one of skin care water, gel, mask, foam, emulsion, cream, serum, sunscreen, barrier cream, primer, foundation cream, BB cream, CC cream, concealer, mascara, eyeliner, lip dye and lip gloss.

[0040] In some embodiments of the present application, the mass fraction of Portulaca oleracea extract in the cosmetic material composition is 0.10wt%-90.00wt%, which can be selected as 1.00wt%-20.00wt%.

[0041] The present application also provides a preparation method of the above-mentioned Portulaca oleracea extract, which comprises the following steps: The pectin-degrading microorganism and white rot fungus are respectively subjected to enrichment culture with Portulaca oleracea powder to obtain pectinase fermentation coenzyme and white rot fungus fermentation coenzyme, which are mixed to form a composite fermentation coenzyme; The Portulaca oleracea powder is subjected to enzymolysis reaction with the composite fermentation coenzyme, and the enzyme is inactivated to obtain the Portulaca oleracea extract.

[0042] Enrichment culture, also known as enrichment culture, refers to a culture method that starts from a mixed group of microorganisms and continuously increases the proportion of a specific species to lead to pure culture.

[0043] In some embodiments of the present application, the cultivation method of pectinase fermentation coenzyme includes: inoculating an inoculum source containing a pectin-degrading microbial community into a culture medium containing purslane powder and performing two enrichment cultures in sequence, wherein the method of each enrichment culture is shaking culture at 25~27°C and 50~100rpm for 1.5~3 days to obtain pectinase fermentation coenzyme; in the present application, the inoculum source can be the soil of a purslane farm.

[0044] In some embodiments of the present application, the cultivation method of white rot fungus fermentation coenzyme includes: inoculating an inoculum source containing white rot fungi into a culture medium containing purslane powder for cultivation, and the cultivation method is shaking cultivation at 25~27°C and 100~200rpm for 10~12 days to obtain white rot fungus fermentation coenzyme; in the present application, the inoculum source can be the mycelium of the white rot fungus Pleurotus ostreatus.

[0045] In some embodiments of the present application, the mass ratio of pectinase fermentation coenzyme and white rot fungus fermentation coenzyme in the composite fermentation coenzyme is 4-6:4-6, and the mass ratio of purslane powder to the composite fermentation coenzyme is 8-12:1; And / or, the enzymatic hydrolysis reaction method includes: first stirring at 28-32° C. and 50-80 rpm for 1.5-2.5 hours, and then stirring at 23-27° C. and 120-180 rpm for 1.5-2.5 hours.

[0046] Hereinafter, the present application will be described in more detail through specific embodiments and experimental examples of the present application.

[0047] Example 1 like Figure 1 As shown, this example provides a purslane extract. To prepare the purslane extract containing purslane plant cells, a soil sample (3 g / L) from a purslane farm was used as an inoculum source. The culture supernatant of a pectin-degrading microbial community that had undergone two enrichment cultures and a white-rot fungus were inoculated (3% wet wt / L). The supernatant of the mycelial culture of the white-rot fungus Pleurotus ostreatus that had undergone a single culture was mixed with two composite fermentation coenzymes. The specific preparation process is as follows: (1) 1st enrichment culture: First, prepare the culture medium and autoclave, then inoculate the soil microorganisms, and then culture at 25 degrees and 70 rpm, and finally filter with non-woven fabric to obtain the first enrichment culture liquid. The process for preparing the enrichment culture fermentation coenzyme is as follows: inoculate the first enrichment culture liquid that has been cultured for 3 days into the second enrichment culture medium (the composition of which is the same as that of the first enrichment culture medium), and culture under the same conditions as those for the first enrichment culture. Filter the second enrichment culture liquid that has been cultured for 3 days with non-woven fabric to obtain the pectinase fermentation coenzyme. First, prepare the culture medium and autoclave, then inoculate the soil microorganisms, and then culture at 25 degrees and 70 rpm, and finally filter with non-woven fabric to obtain the first enrichment culture liquid. The process for preparing the enrichment culture fermentation coenzyme is as follows: inoculate the first enrichment culture liquid that has been cultured for 3 days into the second enrichment culture medium (the composition of which is the same as that of the first enrichment culture medium), and culture under the same conditions as those for the first enrichment culture. Filter the second enrichment culture liquid that has been cultured for 3 days with non-woven fabric to obtain the pectinase fermentation coenzyme. First, prepare the culture medium and autoclave, then inoculate the soil microorganisms, and then culture at 25 degrees and 70 rpm, and finally filter with non-woven fabric to obtain the first enrichment culture liquid. The process for preparing the enrichment culture fermentation coenzyme is as follows: inoculate the first enrichment culture liquid that has been cultured for 3 days into the second enrichment culture medium (the composition of which is the same as that of the first enrichment culture medium), and culture under the same conditions as those for the first enrichment culture. Filter the second enrichment culture liquid that has been cultured for 3 days with non-woven fabric to obtain the pectinase fermentation coenzyme. First, prepare the culture medium and autoclave, then inoculate the soil microorganisms, and then culture at 25 degrees and 70 rpm, and finally filter with non-woven fabric to obtain the first enrichment culture liquid. The process for preparing the enrichment culture fermentation coenzyme is as follows: inoculate the first enrichment culture liquid that has been cultured for 3 days into the second enrichment culture medium (the composition of which is the same as that of the first enrichment culture medium), and culture under the same conditions as those for the first enrichment culture. Filter the second enrichment culture liquid that has been cultured for 3 days with non-woven fabric to obtain the pectinase fermentation coenzyme.

[0048] (2) Second enrichment culture: First, prepare the culture medium and autoclave, then inoculate the soil microorganisms, and then culture at 25 degrees and 70 rpm, and finally filter with non-woven fabric to obtain the first enrichment culture liquid. The process for preparing the enrichment culture fermentation coenzyme is as follows: inoculate the first enrichment culture liquid that has been cultured for 3 days into the second enrichment culture medium (the composition of which is the same as that of the first enrichment culture medium), and culture under the same conditions as those for the first enrichment culture. Filter the second enrichment culture liquid that has been cultured for 3 days with non-woven fabric to obtain the pectinase fermentation coenzyme.

[0049] (3) Preparation of white rot fungus fermentation coenzyme: First, prepare the culture medium and autoclave, then inoculate the white rot fungus, then culture at 25 degrees and 150 rpm, and finally filter with non-woven fabric to obtain the culture liquid. The specific process is as follows: for the white rot fungus, which is the oyster mushroom, inoculate it into the culture medium containing the spinach powder (2 g / L, the culture medium includes 88 g / L of 80 mesh spinach powder and 10 g / L of glucose), and culture at 25°C and 150 rpm for 12 days. Then, filter the culture supernatant with non-woven fabric to recover the white rot fungus fermentation coenzyme, which can be used as additional fermentation coenzyme.

[0050] (4) Preparation of spinach extract containing spinach plant cells: The two kinds of prepared fermentation coenzymes (pectinase fermentation coenzyme: white rot fungus fermentation coenzyme) were mixed in a ratio of 5:5 to complete the fermentation coenzyme complex treatment; the complex fermentation coenzyme was mixed with the purslane powder in a ratio of 10 times the weight of the purslane powder (the purslane powder was obtained by first immersing purslane leaves / stems in salt water, then immersing twice in purified water, and then drying in a cold air at 10 degrees and pulverizing), and stirring extraction was performed (30°C, stirring at 60 rpm, 2 hours); fermentation coenzyme inactivation was performed at a temperature of 70°C for 1 hour, and after additional stirring extraction (150 rpm, 25°C, 2 hours), filtration was performed through a bag filter having a pore size of 150 μm, the filtrate was recovered, and a purslane extract was obtained.

[0051] Microscopic detection was performed on the purslane extract, and as shown in FIG. 1, it can be seen that the purslane plant cells are intact. Figure 2

[0052] Example 2 This example provides a cosmetic composition, and the preparation process is as follows: In order to understand the soothing and irritation-relieving effects by preparing the emulsion of the present application containing the purslane extract (Example 1) containing purslane plant cells, an emulsion was prepared using the formulation shown in Table 1. The contents in the emulsion formulation are expressed in weight %.

[0053] Table 1 Emulsion formulation of Example 2 to which the purslane extract containing purslane plant cells is added

[0054] Preparation method: The raw materials 1~2 were weighed together, and under normal temperature conditions, the water phase was prepared by stirring with a stirrer while adding raw material 3. The water phase was stirred with an emulsifier while adding the additionally weighed and uniformly mixed raw materials 4~5 (oil phase), and emulsification was performed at 2000 rpm for 10 minutes. After adding raw material 6 to thicken, raw material 7 was finally added, and after uniform stirring, it was stored in a closed state.

[0055] Example 3 This example provides a cosmetic material composition, which is different from Example 2 in that the content of the purslane extract containing purslane plant cells is 0.10 wt%.

[0056] Example 4 This example provides a cosmetic material composition, which is different from Example 2 in that the content of the purslane extract containing purslane plant cells is 90 wt%.

[0057] Comparative Example 1 As shown in FIG. 1, it can be seen that the purslane plant cells are intact. Figure 1 ​As shown, the present comparative example provides a purslane extract not containing purslane plant cells, which is prepared as follows: After preparing the purslane extract containing purslane plant cells in the same manner as in Example 1, all of the purslane plant cells contained were broken by stirring at a high speed of 20,000 rpm, and all of the broken purslane plant cells were removed by using a tube centrifuge. In this way, the purslane extract not containing purslane plant cells was finally prepared.

[0058] Comparative Example 2 The present comparative example provides an emulsion containing a purslane extract not containing purslane plant cells, which is prepared as follows: In the cosmetic material, as a control group for comparison with the skin soothing (stimulus alleviation evaluation) of the purslane plant cells of the present application, the emulsion containing the purslane extract not containing purslane plant cells (Comparative Example 2) was also prepared with the formulation shown in Table 2. The contents in the emulsion formulation are expressed in weight %.

[0059] Table 2 Emulsion formulation of Comparative Example 2 to which the purslane extract not containing purslane plant cells is added

[0060] Preparation method: The raw materials 1~2 were weighed together, and the water phase was prepared by stirring with a stirrer at room temperature while adding the raw material 3. The water phase was stirred with an emulsifier while adding the other raw materials 4~5 (oil phase) which were weighed together and uniformly mixed, and emulsified for 10 minutes at 2,000 rpm. After thickening by adding the raw material 6 thereto, the raw material 7 was finally added, and after uniform stirring, it was stored in a closed state.

[0061] Experimental Example 1: Antioxidant ability of the purslane extract containing purslane plant cells (evaluation of DPPH radical scavenging ability) In order to understand the antioxidant effect of the purslane extract containing purslane plant cells (Example 1) on the skin, the evaluation of DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging ability was performed. Example 1 and Comparative Example 1 were each put into a 24-well plate, and DPPH prepared with a 100 μM ethanol solution was added so that the final volume of the test solution was 200 μl. After the test solution was left to stand for 30 minutes at room temperature and in the dark, the absorbance was measured using an ELISA reader at 570 nm. The DPPH radical scavenging activity (%) was calculated according to Equation 1 below.

[0062] Equation 1: DPPH radical scavenging rate (%) = (absorbance of test group - absorbance of sample) / absorbance of control group x 100 As shown in Table 2, the results indicate that the purslane extract containing purslane plant cells (Example 1) and the purslane extract not containing purslane plant cells (Comparative Example 1) showed higher antioxidant capacity than the positive control group, and the purslane extract containing purslane plant cells (Example 1) showed higher antioxidant capacity than the purslane extract not containing purslane plant cells (Comparative Example 1). Thus, it was confirmed that the purslane extract containing purslane plant cells (Example 1) had higher antioxidant effect than the purslane extract not containing ordinary purslane plant cells (Comparative Example 1). Figure 3 As shown in Table 2, the results indicate that the purslane extract containing purslane plant cells (Example 1) and the purslane extract not containing purslane plant cells (Comparative Example 1) showed higher antioxidant capacity than the positive control group, and the purslane extract containing purslane plant cells (Example 1) showed higher antioxidant capacity than the purslane extract not containing purslane plant cells (Comparative Example 1). Thus, it was confirmed that the purslane extract containing purslane plant cells (Example 1) had higher antioxidant effect than the purslane extract not containing ordinary purslane plant cells (Comparative Example 1).

[0063] Experimental Example 2: Evaluation of Nitric Oxide (NO) Generation Inhibitory Effect of Purslane Extract Containing Purslane Plant Cells To understand the anti-inflammatory effect of the purslane extract containing purslane plant cells on skin, an evaluation of the nitric oxide (NO) generation inhibitory effect was performed. Raw 264.7 (mouse macrophage) cells were treated with Example 1 and Comparative Example 1, respectively, together with lipopolysaccharide (LPS) to induce the generation of nitric oxide (NO). Then, the degree of inhibition of the generation of nitric oxide based on lipopolysaccharide (LPS) by Example 1 and Comparative Example 1 was confirmed. In the experiment, a sample showing an inhibitory effect of 50% or more on the generation of nitric oxide was judged to have an inhibitory effect on the generation of nitric oxide, relative to total NOS inhibitor (L-NMMA) as a positive control group.

[0064] As shown in Table 3, the results indicate that Example 1 and Comparative Example 1 showed higher inhibitory effect on the generation of nitric oxide than the positive control group, and Example 1 showed higher inhibitory effect on the generation of nitric oxide than Comparative Example 1. Thus, it was confirmed that the purslane extract containing purslane plant cells had higher anti-inflammatory efficacy than the purslane extract not containing ordinary purslane plant cells.

[0065] Table 3: Results of inhibitory effect on the generation of nitric oxide of different samples

[0066] Experimental Example 3: Evaluation of TNF-α Expression Inhibitory Ability of Purslane Extract Containing Purslane Plant Cells To understand the anti-inflammatory effect of the purslane extract containing purslane plant cells (Example 1) on skin, an evaluation of the TNF-α expression inhibitory ability, which is one of proinflammatory factors, was performed. Human-derived keratinocytes (HaCaT) were treated with Example 1 and Comparative Example 1, respectively, at a concentration of 1 x 10 5cells / ml were inoculated into 24-well plates and cultured for 24 hours at 12.5 mJ / cm 2 The cells were irradiated with ultraviolet B radiation at an intensity of 100 nm. Subsequently, a purslane extract containing purslane plant cells (Example 1) and a purslane extract without purslane plant cells (Comparative Example 2), 5 μg / ml of 99.5% dichloromethane, and 5 μg / ml of 99% ethyl acetate were added to the plate and incubated for 24 hours. The amount of TNF-α secreted in the culture medium was then measured by immunoassay.

[0067] like Figure 4 As shown, the results showed that the expression of "TNF-α" was inhibited in both the purslane extract containing purslane plant cells (Example 1) and the purslane extract not containing purslane plant cells (Comparative Example 1), and the effect of the purslane extract containing purslane plant cells (Example 1) was more excellent.

[0068] Experimental Example 4: Evaluation of the IL-2 Expression Inhibitory Ability of Portulaca Oleracea Extract Containing Portulaca Oleracea Plant Cells To investigate the anti-inflammatory effects of a raw material, a Portulaca oleracea extract containing Portulaca oleracea plant cells (Example 1), on the skin, we evaluated its ability to inhibit the expression of IL-2, a pro-inflammatory cytokine. The experimental method was the same as in Experimental Example 3, and after 24 hours of culture, the amount of IL-2 secreted in the culture medium was measured by immunoassay.

[0069] like Figure 5 As shown, the results showed that both the purslane extract containing purslane plant cells (Example 1) and the purslane extract without purslane plant cells (Comparative Example 1) suppressed IL-2 expression, with the purslane extract containing purslane plant cells (Example 1) having a superior effect. The results of Experimental Examples 2 to 4 ultimately demonstrated that the purslane extract containing purslane plant cells reduced the production and expression of factors that induce inflammation, and confirmed that the purslane extract containing purslane plant cells (Example 1) had a superior skin-soothing effect compared to the purslane extract without purslane plant cells (Comparative Example 1).

[0070] Experimental Example 5: Evaluation of skin irritation relief of a lotion containing a purslane extract containing purslane plant cells In order to understand the skin soothing effect of the purslane extract containing purslane plant cells (Example 1) on the skin, an evaluation of the skin irritation relief efficacy based on SLS was conducted (Patch test - MEXAMETER ® ). Using MEXAMETER ®Erythema meter measured the erythema value (when measuring, the temperature was 20°C to 25°C, the humidity was 40% to 50%, and the measurement was repeated twice). After placing a 1.5 cm x 1.5 cm size of sterile gauze on the forearm site of the subject, 300 μl of 3% sodium lauryl sulfate (SLS) solution was treated, and irritation was induced for 30 minutes. Then, the sodium lauryl sulfate solution (SLS solution) was washed with running water, and rested for 10 minutes. The emulsion of the spina chia extract (Example 1) added with spina chia plant cells (Example 2) and the emulsion of the spina chia extract (Comparative Example 1) added with no spina chia plant cells (Comparative Example 2) were gently applied to the same site with a sterile cotton swab, and then, after 24 hours, the degree of irritation relief was observed. The results are shown in Table 4 (performed on 4 men aged 20 to 30 years and 6 women aged 20 to 30 years, after evaluation, the average of the average erythema values of 6 except for 2 maximum erythema values and 2 minimum erythema values was marked). The results showed that the emulsion of the spina chia extract (Example 1) added with spina chia plant cells (Example 2) reduced the erythema value of SLS irritation more than the emulsion of the spina chia extract (Comparative Example 1) added with no spina chia plant cells (Comparative Example 2). Therefore, it was known that the spina chia extract added with spina chia plant cells was more effective in irritation relief than the spina chia extract added with no ordinary spina chia plant cells.

[0071] Table 4 Results of the degree of irritation relief of different samples

[0072] Experimental Example 6: For the soil samples of 3 spina chia farms, after crushing and adding spina chia raw materials and secondly enriching and culturing, the non-woven fabric was filtered, and then the pectinase activity was measured, the pectinase activity was measured every 12 hours for 3 days, 3 different soil samples from different places were cultured and the pectinase activity was measured, and the results are shown in Table 6. Figure 6

[0073] From Figure 6 ​As can be seen in Table 1, the pectinase activity of the three samples reached the maximum after 36 hours of cultivation. Therefore, the pectinase coenzyme was prepared using the sample obtained after 36 hours of the second enrichment cultivation. In addition, the three soil samples showed similar enzyme activity in the enrichment culture broth for 72 hours, and it is expected that similar pectinase coenzyme broth will be obtained when re-cultured. The coenzyme obtained after 72 hours of cultivation was mixed with the coenzyme of the Pleurotus ostreatus mycelium culture at a ratio of 5:5 (v / v) and used. The ratio of the two coenzyme liquids was 5:5.

[0074] In the above process, the pectinase activity was measured by adding 15 µl of the microbial-removed culture supernatant to 250 µl of 50 mM Tris-Cl (pH 8.0) buffer to which 0.5% (w / v) pectin was added. Then, it was reacted in a water bath at 50°C for 30 minutes, and after adding 500 µl of 1N NaOH again, it was reacted at 75°C for 10 minutes, and the entire reaction was terminated. To the reaction-terminated reaction liquid, 6000 µl of 1N HCl to which 0.04M 2-thiobarbituric acid was added was added, and it was reacted again at 76°C for 10 minutes. At this time, the absorbance was measured at A550nm with respect to the generated pink color. One pectinase activity unit is the amount of pectinase required to increase the absorbance by 0.01 at A550nm.

[0075] Experimental Example 7: The Pleurotus ostreatus mycelium was cultured for 12 days, and the enzyme activity of the lignin-degrading enzyme group was measured every 2 days. The specific measurement method was as follows: Lignin peroxidase (LiP) enzyme activity: It was confirmed by the method of Tien and Kirk (1983). The activity concentration is the degree to which 1 nmol of veratryl alcohol is oxidized to veratryl aldehyde in 1 minute, which is expressed as absorbance at a wavelength of 310 nm.

[0076] Manganese peroxidase (Mn-dependent peroxidase, MnP) enzyme activity: It was confirmed by the method of Kofujita et al. (1991). The activity concentration is expressed as the absorbance at 465 nm of the substance generated in 1 minute of reaction.

[0077] Laccase (Lac) enzyme activity: Determined using the method of Kofujita et al. (1991). The activity concentration is expressed as the absorbance at 440 nm of the product generated after one minute of reaction using o-phenylendiamine as the substrate.

[0078] The results are as follows Figure 7 As shown, from Figure 7 As can be seen from the results, LiP enzyme activity was absent, and laccase and MnP showed their highest activity on day 10. Therefore, the 10-day culture of Pleurotus ostreatus mycelium was mixed with the prepared pectinase coenzyme. The mixing ratio of the two coenzyme solutions was 5:5.

[0079] The present application is described in detail through the above embodiments and experimental examples, but the scope of rights of the present application is not limited thereto. It is obvious to ordinary technicians in this technical field that various modifications and variations can be made without departing from the scope of the technical ideas of the present application recorded in the claims.

Claims

1. A purslane extract, characterized in that Contains Purslane plant cells.

2. The purslane extract according to claim 1, characterized in that The purslane extract is obtained by treating purslane with an enzymatic hydrolysis method.

3. The purslane extract according to claim 1, characterized in that Based on the mass of the purslane extract, the mass proportion of the purslane plant cells is 0.10wt%~90.00wt%, and can be optionally 0.5wt%~10.00wt%.

4. A use of the Portulaca oleracea extract according to any one of claims 1 to 3, characterized in that: The purslane extract is used as a cosmetic raw material having anti-oxidation, anti-inflammatory and soothing effects on the skin.

5. A cosmetic material composition, characterized in that: The method comprises the purslane extract according to any one of claims 1 to 3.

6. The cosmetic material composition according to claim 5, characterized in that The cosmetic material composition is selected from any one of skin care lotion, gel, facial mask, foam, lotion, facial cream, essence, sunscreen, isolation cream, primer, foundation cream, BB cream, CC cream, concealer, mascara, eyeliner, lip stain and lip gloss.

7. The cosmetic material composition according to claim 5, characterized in that Based on the mass of the cosmetic material composition, the mass proportion of the purslane extract is 0.10wt%~90.00wt%, and can be optionally 1.00wt%~20.00wt%.

8. A method for preparing the Portulaca oleracea extract according to any one of claims 1 to 3, characterized in that: It includes the following steps: Pectin-degrading microorganisms, white-rot fungi and purslane powder are enriched and cultured respectively to obtain pectinase fermentation coenzyme and white-rot fungi fermentation coenzyme, which are then mixed to form a composite fermentation coenzyme. The purslane powder is subjected to enzymatic hydrolysis reaction with the composite fermentation coenzyme, and the enzyme is inactivated to obtain the purslane extract.

9. The method for preparing the Portulaca oleracea extract according to claim 8, wherein: The method for culturing pectinase fermentation coenzyme comprises: inoculating an inoculum containing a pectin-degrading microbial community into a culture medium containing purslane powder and sequentially performing two enrichment cultures, wherein each enrichment culture is performed at 25-27° C. and 50-100 rpm with shaking for 1.5-3 days to obtain pectinase fermentation coenzyme; And / or, the method for culturing the white rot fungus fermentation coenzyme comprises: inoculating an inoculum containing white rot fungi into a culture medium containing purslane powder for culturing, wherein the culturing method is shaking culturing at 25-27° C. and 100-200 rpm for 10-12 days to obtain the white rot fungus fermentation coenzyme.

10. The method for preparing the Portulaca oleracea extract according to claim 8, wherein: The mass ratio of the pectinase fermentation coenzyme and the white rot fungus fermentation coenzyme in the composite fermentation coenzyme is 4-6:4-6, and the mass ratio of the purslane powder to the composite fermentation coenzyme is 8-12:1; And / or, the enzymatic hydrolysis reaction method includes: first stirring at 28-32° C. and 50-80 rpm for 1.5-2.5 hours, and then stirring at 23-27° C. and 120-180 rpm for 1.5-2.5 hours.

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