An oil control acne-removing and pore-shrinking composition, use and skin care product

Abstract

The application belongs to the technical field of skin care, and discloses an oil-controlling, acne-removing and pore-shrinking composition, application and skin care product. The oil-controlling, acne-removing and pore-shrinking composition comprises oyster extract and pomegranate extract. The oyster extract is obtained by extracting 1-8 parts of oysters, the pomegranate extract is obtained by extracting 0.1-5 parts of pomegranates, the composition further comprises 5-15 parts of fishwort extract obtained by extracting fishwort and 0.5-10 parts of rose bataleur flower extract obtained by extracting rose bataleur flowers. The oil-controlling, acne-removing and pore-shrinking composition provided by the application can effectively improve the oil-controlling and bacteriostatic effects of the composition by means of oyster extract and pomegranate extract, so as to achieve the effects of oil control, acne removal and pore shrinkage.

Description

Technical Field

[0001] This application relates to the field of skin care technology, specifically to an oil-controlling, acne-reducing, and pore-shrinking composition, its uses, and skin care products. Background Technology

[0002] Oyster extract, with its high content of zinc, selenium, active peptides, and Omega-3, has become a popular ingredient in skincare products due to its complex effects such as firming the skin, anti-oxidation, regulating oil-water balance, and repairing the skin. Oyster extract can significantly promote collagen synthesis and enhance skin elasticity, while also providing powerful antioxidants to reduce wrinkles and age spots. Its oil-controlling and anti-inflammatory properties can regulate the water-oil balance and tighten pores, while natural amino acids and minerals deeply moisturize and repair the skin barrier, making it especially suitable for sensitive and mature skin.

[0003] Therefore, oyster extract can be used in skincare products with various effects. For example, prior art 1: KR1020070126284 discloses a method for extracting useful components from oysters, which obtains high-purity oyster extract of useful components through ultrasonic treatment, ethanol precipitation, and filtration. Furthermore, it provides a skin-whitening cosmetic composition containing this composition to prevent the formation of melanin and decompose existing melanin.

[0004] Existing technology 1 uses oyster extract to form a whitening composition and achieves a good skin-enhancing effect; however, existing technology 1 only focuses on skin whitening and does not further explore other effects of oyster extract.

[0005] Controlling oil production is crucial for maintaining healthy skin, and its importance is reflected in three aspects: First, excessive sebum secretion can clog pores, leading to inflammatory problems such as acne and blackheads; second, oily skin can easily cause dull skin tone and makeup to look messy, affecting appearance and social confidence; and more importantly, sebum oxidation can accelerate skin aging.

[0006] Existing technologies have not explored the oil-controlling effects of oyster extract, and no effective oil-controlling compositions have been developed based on oyster extract. Summary of the Invention

[0007] One of the purposes of this application is to provide an oil-controlling, acne-removing, and pore-shrinking composition, which is formed around oyster extract and has excellent oil-controlling and acne-removing effects.

[0008] Another objective of this application is to provide the use of an oil-controlling, acne-removing, and pore-shrinking composition, which, when prepared into a skin care product, has excellent oil-controlling and acne-removing effects.

[0009] In addition, this application also provides a skin care product that has oil-controlling and acne-reducing effects.

[0010] To achieve the above objectives, this application provides an oil-controlling, acne-removing, and pore-shrinking composition, comprising oyster extract and pomegranate extract. By weight, the oyster extract is obtained from 1 to 8 parts of oyster extract, and the pomegranate extract is obtained from 0.1 to 5 parts of pomegranate extract.

[0011] This application found that by combining oyster extract and pomegranate extract, the two have a synergistic effect. At the same concentration, the combination of the two has a better oil control and Propionibacterium acnes inhibition effect.

[0012] Preferably, it also includes houttuynia cordata extract obtained from 5 to 15 parts of houttuynia cordata extract and hibiscus flower extract obtained from 0.5 to 10 parts of hibiscus flower extract.

[0013] This application, based on the compound of oyster extract and pomegranate extract, further adds houttuynia cordata extract and hibiscus flower extract, which can significantly enhance the oil control and Propionibacterium acnes inhibition effects of the composition.

[0014] It should be noted that the oyster extract, pomegranate extract, houttuynia cordata extract, and hibiscus flower extract of this application can be obtained from commercially available products, and can also achieve corresponding synergistic effects through compound formulation; more preferably, the oyster extract, pomegranate extract, houttuynia cordata extract, and hibiscus flower extract of this application are prepared by the following method:

[0015] Preferably, the oyster extract and pomegranate extract are obtained by the following method:

[0016] Step a: Crush the oysters into powder and add them to 8-13 BV of water. Stir and extract at 80±5℃ for 2-4 hours, then centrifuge to obtain the oyster extract.

[0017] Step b: Then, the oyster extract is concentrated to obtain a concentrate, wherein the mass ratio of the concentrate to the oyster extract is 1:5~11;

[0018] Step c: After crushing the pomegranate into powder, add it to 10~20 BV of ethanol solution with a concentration of 60%, stir and extract at 60±5℃ for 2~3 hours, and centrifuge to obtain pomegranate extract.

[0019] Step d: Concentrate the pomegranate extract until it is alcohol-free to obtain alcohol-free concentrate A. Mix the concentrate obtained in step b with alcohol-free concentrate A, and then add polyol and water to obtain mixed solution A; the polyol content in mixed solution A is 30 wt%.

[0020] Step e: Dissolve the mixed solution A by stirring and then ultrafiltration.

[0021] The oil-controlling, acne-reducing, and pore-minimizing composition was obtained by the following method:

[0022] Step a: Crush the oysters into powder and add them to 8-13 BV of water. Stir and extract at 80±5℃ for 2-4 hours, then centrifuge to obtain the oyster extract.

[0023] Step b: Then, the oyster extract is concentrated to obtain a concentrate, wherein the mass ratio of the concentrate to the oyster extract is 1:5~11;

[0024] Step c: After crushing the pomegranate into powder, add it to 10~20 BV of ethanol solution with a concentration of 60%, stir and extract at 60±5℃ for 2~3 hours, centrifuge to obtain pomegranate extract; concentrate the pomegranate extract until alcohol-free to obtain alcohol-free concentrate A;

[0025] Step d: After crushing Houttuynia cordata into powder, add it to 10~20 BV of ethanol solution with a concentration of 60%, stir and extract at 60±5℃ for 2~3 hours, centrifuge to obtain Houttuynia cordata extract; concentrate the Houttuynia cordata extract to alcohol-free solution B.

[0026] Step e: After crushing the hibiscus flowers into powder, add them to 10-20 BV of 60% ethanol solution and stir and extract at 60±5℃ for 2-3 hours. Centrifuge to obtain hibiscus flower extract; concentrate the hibiscus flower extract to alcohol-free solution C.

[0027] Step f: Mix the concentrated solution, alcohol-free concentrated solution A, alcohol-free concentrated solution B, and alcohol-free concentrated solution C, then add polyol and water to obtain mixed solution B; the polyol content in mixed solution B is 30 wt%.

[0028] Step g: Dissolve the mixed solution B by stirring and then ultrafiltration.

[0029] In this invention, BV refers to bed volume, abbreviated as BV. In chemical production, resins are commonly used. The amount of solution added to a resin column is determined by the amount of resin packed in it; 1L of resin passing through 1L of solution is called 1BV. This concept has been extended in the plant extraction industry, typically used to represent the weight of extraction solvent added based on the weight of the raw material. It can also be called a multiple, for example, adding 10kg of water to 1kg of raw material for extraction is recorded as 10BV. This method allows for a clear understanding of the relationship between the amount of raw material and the amount of solvent used in product development and production.

[0030] Furthermore, the specific operation of stirring and dissolving is as follows: stirring and dissolving at 600~750 rpm for 0.5~1 h at room temperature; the specific operation of ultrafiltration is as follows: ultrafiltration is performed at an ultrafiltration pressure of 0.20~0.30 MPa, controlling the ultrafiltration temperature to not exceed 35℃, and ultrafiltration membrane pore size to be 0.1~0.45 μm to obtain the filtrate.

[0031] Furthermore, the specific operation of the concentration is as follows: rotary evaporation is performed under the conditions of a temperature of 50~60℃, a pressure of -0.08~-0.1MPa, and a rotation speed of 40~60r / min.

[0032] Preferably, the oil-controlling, acne-removing, and pore-shrinking composition may also contain 2wt% preservative, and then be sterilized at 85°C for 1 hour.

[0033] Furthermore, the preservative is at least one selected from 1,2-hexanediol, p-hydroxyacetophenone, 1,2-pentanediol, octyl glycol, ethylhexylglycerin, and capryloyl hydroxamic acid.

[0034] This application also provides the use of the above-described oil-controlling, acne-reducing, and pore-minimizing composition in the preparation of skin care products.

[0035] This application also discloses a skin care product containing 0.01 to 30 wt% of the above-mentioned oil-controlling, acne-removing, and pore-shrinking composition.

[0036] Beneficial effects

[0037] Compared with the prior art, this application provides an oil-controlling, acne-removing, and pore-shrinking composition. Oyster extract and pomegranate extract can effectively enhance the oil-controlling and antibacterial effects of the composition, thereby achieving the effects of oil control, acne removal, and pore shrinking. Attached Figure Description

[0038] The present invention will be further described below with reference to the accompanying drawings and embodiments;

[0039] Figure 1 This is a picture of the chicken embryo before use in Embodiment 4 of the present invention;

[0040] Figure 2 This is a picture of a chicken embryo after use in Embodiment 4 of the present invention. Detailed Implementation

[0041] The present application will be further described below with reference to embodiments, but this does not constitute any limitation on the present application. Any limited modifications made within the scope of the claims of the present application shall still be within the scope of the claims of the present application.

[0042] To illustrate the technical content of this application in detail, the following description is provided in conjunction with the embodiments.

[0043] In the following examples and comparative examples, the oyster meat and pomegranate were purchased from Xihua Meat and Vegetable Market in Guangzhou, Guangdong Province; the roselle flower (trade name: Hibiscus rosa-sinensis) was purchased from Pocket Doctor Biotechnology Co., Ltd. in Bozhou City; and the houttuynia cordata was purchased from Yuanshengtang Pharmaceutical Co., Ltd. in Bozhou City.

[0044] In the following examples and comparative examples, the specific process of concentration is as follows: rotary evaporation at a temperature of 55±5℃, a pressure of -0.09MPa, and a rotation speed of 50r / min.

[0045] Example 1

[0046] An oil-controlling, acne-reducing, and pore-minimizing composition is prepared using the following steps:

[0047] Step 1: Crush 50g of oysters into powder and add it to 550g of water. Stir and extract at 80±5℃ for 3 hours. Centrifuge to obtain oyster extract and concentrate the oyster extract to 100g concentrate.

[0048] Step 2: Crush 30g of pomegranate into powder and add it to 300g of 60% ethanol solution. Stir and extract at 60±5℃ for 3h, and centrifuge to obtain pomegranate extract. Concentrate the pomegranate extract until it is alcohol-free to obtain 60g of alcohol-free concentrate A.

[0049] Step 3: Mix the concentrated solution with alcohol-free concentrated solution A, add 300g butanediol and 540g water, and then stir at 750rpm for 1 hour at room temperature to dissolve. Then, use an ultrafiltration membrane with a pore size of 0.1um to ultrafilter the solution and obtain the oil-controlling, acne-removing and pore-shrinking composition.

[0050] Example 2

[0051] An oil-controlling, acne-reducing, and pore-minimizing composition is prepared using the following steps:

[0052] Step 1: Crush 20g of oyster meat into powder and add it to 220g of water. Stir and extract at 80±5℃ for 3 hours. Centrifuge to obtain oyster extract and concentrate the oyster extract to 40g concentrate.

[0053] Step 2: Crush 50g of pomegranate into powder and add it to 500g of 60% ethanol solution. Stir and extract at 60±5℃ for 3h, and centrifuge to obtain pomegranate extract. Concentrate the pomegranate extract to alcohol-free solution to obtain 100g of alcohol-free concentrate A.

[0054] Step 3: Mix the concentrated solution with alcohol-free concentrated solution A, add 300g butanediol and 560g water, and then stir at 750rpm for 1 hour at room temperature to dissolve. Then, use an ultrafiltration membrane with a pore size of 0.1um to ultrafilter the solution and obtain the oil-controlling, acne-removing and pore-shrinking composition.

[0055] Example 3

[0056] An oil-controlling, acne-reducing, and pore-minimizing composition is prepared using the following steps:

[0057] Step 1: Crush 70g of oyster meat into powder and add it to 770g of water. Stir and extract at 80±5℃ for 3 hours. Centrifuge to obtain oyster extract and concentrate the oyster extract to 140g concentrate.

[0058] Step 2: Crush 5g of pomegranate into powder and add it to 50g of 60% ethanol solution. Stir and extract at 60±5℃ for 3h, and centrifuge to obtain pomegranate extract. Concentrate the pomegranate extract to alcohol-free solution to obtain 10g of alcohol-free concentrate A.

[0059] Step 3: Mix the concentrated solution with alcohol-free concentrated solution A, add 300g butanediol and 550g water, and then stir at 750rpm for 1 hour at room temperature to dissolve. Then, use an ultrafiltration membrane with a pore size of 0.1um to ultrafilter the solution and obtain the oil-controlling, acne-removing and pore-shrinking composition.

[0060] Example 4

[0061] An oil-controlling, acne-reducing, and pore-minimizing composition is prepared using the following steps:

[0062] Step 1: Crush 50g of oyster meat into powder and add it to 550g of water. Stir and extract at 80±5℃ for 3 hours. Centrifuge to obtain oyster extract and concentrate the oyster extract to 100g concentrate.

[0063] Step 2: Crush 30g of pomegranate into powder and add it to 300g of 60% ethanol solution. Stir and extract at 60±5℃ for 3h, and centrifuge to obtain pomegranate extract. Concentrate the pomegranate extract until it is alcohol-free to obtain 60g of alcohol-free concentrate A.

[0064] Step 3: Crush 80g of Houttuynia cordata into powder and add it to 800g of 60% ethanol solution. Stir and extract at 60±5℃ for 3h. Centrifuge to obtain Houttuynia cordata extract. Concentrate the Houttuynia cordata extract to alcohol-free solution to obtain 160g of alcohol-free concentrate B.

[0065] Step 4: Crush 40g of hibiscus flowers into powder and add them to 400g of 60% ethanol solution. Stir and extract at 60±5℃ for 3h. Centrifuge to obtain hibiscus flower extract. Concentrate the hibiscus flower extract to alcohol-free solution to obtain 80g of alcohol-free concentrate C.

[0066] Step 5: Mix the concentrated solution, alcohol-free concentrated solution A, alcohol-free concentrated solution B and alcohol-free concentrated solution C, add 300g of butanediol and 300g of water, and then stir at 750rpm for 1 hour at room temperature to dissolve. Then, use an ultrafiltration membrane with a pore size of 0.1um to ultrafilter the solution and obtain the oil-controlling, acne-removing and pore-shrinking composition.

[0067] Example 5

[0068] An oil-controlling, acne-reducing, and pore-minimizing composition is prepared using the following steps:

[0069] Step 1: Crush 20g of oyster meat into powder and add it to 220g of water. Stir and extract at 80±5℃ for 3 hours. Centrifuge to obtain oyster extract and concentrate the oyster extract to 40g concentrate.

[0070] Step 2: Crush 10g of pomegranate into powder and add it to 100g of 60% ethanol solution. Stir and extract at 60±5℃ for 3h, and centrifuge to obtain pomegranate extract. Concentrate the pomegranate extract to alcohol-free solution to obtain 20g of alcohol-free concentrate A.

[0071] Step 3: Crush 120g of Houttuynia cordata into powder and add it to 1200g of 60% ethanol solution. Stir and extract at 60±5℃ for 3h. Centrifuge to obtain Houttuynia cordata extract. Concentrate the Houttuynia cordata extract to alcohol-free solution to obtain 240g of alcohol-free concentrate B.

[0072] Step 4: Crush 80g of hibiscus flowers into powder and add them to 800g of 60% ethanol solution. Stir and extract at 60±5℃ for 3h. Centrifuge to obtain hibiscus flower extract. Concentrate the hibiscus flower extract to alcohol-free solution to obtain 160g of alcohol-free concentrate C.

[0073] Step 5: Mix the concentrated solution, alcohol-free concentrated solution A, alcohol-free concentrated solution B and alcohol-free concentrated solution C, add 300g butanediol and 240g water, and then stir at 750rpm for 1 hour at room temperature to dissolve. Then, use an ultrafiltration membrane with a pore size of 0.1um to ultrafilter the solution and obtain the oil-controlling, acne-removing and pore-shrinking composition.

[0074] Example 6

[0075] An oil-controlling, acne-reducing, and pore-minimizing composition is prepared using the following steps:

[0076] Step 1: Crush 20g of oyster meat into powder and add it to 220g of water. Stir and extract at 80±5℃ for 3 hours. Centrifuge to obtain oyster extract and concentrate the oyster extract to 40g concentrate.

[0077] Step 2: Crush 10g of pomegranate into powder and add it to 100g of 60% ethanol solution. Stir and extract at 60±5℃ for 3h, and centrifuge to obtain pomegranate extract. Concentrate the pomegranate extract to alcohol-free solution to obtain 20g of alcohol-free concentrate A.

[0078] Step 3: Crush 60g of Houttuynia cordata into powder and add it to 600g of 60% ethanol solution. Stir and extract at 60±5℃ for 3h. Centrifuge to obtain Houttuynia cordata extract. Concentrate the Houttuynia cordata extract to alcohol-free solution to obtain 120g of alcohol-free concentrate B.

[0079] Step 4: Crush 20g of hibiscus flowers into powder and add them to 200g of 60% ethanol solution. Stir and extract at 60±5℃ for 3h. Centrifuge to obtain hibiscus flower extract. Concentrate the hibiscus flower extract to alcohol-free solution to obtain 40g of alcohol-free concentrate C.

[0080] Step 5: Mix the concentrated solution, alcohol-free concentrated solution A, alcohol-free concentrated solution B and alcohol-free concentrated solution C, add 300g butanediol and 480g water, and then stir at 750rpm for 1 hour at room temperature to dissolve. Then, use an ultrafiltration membrane with a pore size of 0.1um to ultrafilter the solution and obtain the oil-controlling, acne-removing and pore-shrinking composition.

[0081] Example 7

[0082] An oil-controlling, acne-reducing, and pore-minimizing composition is prepared using the following steps:

[0083] Step 1: Crush 50g of oyster meat into powder and add it to 550g of water. Stir and extract at 80±5℃ for 3 hours. Centrifuge to obtain oyster extract and concentrate the oyster extract to 100g concentrate.

[0084] Step 2: Crush 30g of pomegranate into powder and add it to 300g of 60% ethanol solution. Stir and extract at 60±5℃ for 3h, and centrifuge to obtain pomegranate extract. Concentrate the pomegranate extract until it is alcohol-free to obtain 60g of alcohol-free concentrate A.

[0085] Step 3: Crush 80g of Houttuynia cordata into powder and add it to 800g of 60% ethanol solution. Stir and extract at 60±5℃ for 3h. Centrifuge to obtain Houttuynia cordata extract. Concentrate the Houttuynia cordata extract to alcohol-free solution to obtain 160g of alcohol-free concentrate B.

[0086] Step 4: Mix the concentrated solution, alcohol-free concentrated solution A and alcohol-free concentrated solution B, add 300g of butylene glycol and 380g of water, and then stir at 750rpm for 1 hour at room temperature to dissolve. Then, use an ultrafiltration membrane with a pore size of 0.1um to ultrafilter the solution and obtain the oil-controlling, acne-removing and pore-shrinking composition.

[0087] Example 8

[0088] An oil-controlling, acne-reducing, and pore-minimizing composition is prepared using the following steps:

[0089] Step 1: Crush 50g of oyster meat into powder and add it to 550g of water. Stir and extract at 80±5℃ for 3 hours. Centrifuge to obtain oyster extract and concentrate the oyster extract to 100g concentrate.

[0090] Step 2: Crush 30g of pomegranate into powder and add it to 300g of 60% ethanol solution. Stir and extract at 60±5℃ for 3h, and centrifuge to obtain pomegranate extract. Concentrate the pomegranate extract until it is alcohol-free to obtain 60g of alcohol-free concentrate A.

[0091] Step 3: Crush 40g of hibiscus flowers into powder and add them to 400g of 60% ethanol solution. Stir and extract at 60±5℃ for 3h. Centrifuge to obtain hibiscus flower extract. Concentrate the hibiscus flower extract to alcohol-free solution to obtain 80g of alcohol-free concentrate B.

[0092] Step 4: Mix the concentrated solution, alcohol-free concentrated solution A and alcohol-free concentrated solution B, add 300g of butanediol and 460g of water, and then stir at 750rpm for 1 hour at room temperature to dissolve. Then, use an ultrafiltration membrane with a pore size of 0.1um to ultrafilter the solution and obtain the oil-controlling, acne-removing and pore-shrinking composition.

[0093] Comparative Example 1

[0094] An oyster extract was prepared using the following steps:

[0095] Step 1: Crush 80g of oyster meat into powder and add it to 880g of water. Stir and extract at 80±5℃ for 3 hours. Centrifuge to obtain oyster extract and concentrate the oyster extract to 160g concentrate.

[0096] Step 2: Add 300g butanediol and 540g water to the concentrate, then stir at 750rpm for 1 hour at room temperature to dissolve. Then, use an ultrafiltration membrane with a pore size of 0.1um to ultrafilter the solution and obtain the oyster extract.

[0097] Comparative Example 2

[0098] A pomegranate extract was prepared using the following steps:

[0099] Step 1: Crush 80g of pomegranate into powder and add it to 800g of 60% ethanol solution. Stir and extract at 60±5℃ for 3h, and centrifuge to obtain pomegranate extract. Concentrate the pomegranate extract to alcohol-free solution to obtain 160g of alcohol-free concentrate.

[0100] Step 2: Add 300g butanediol and 540g water to the alcohol-free concentrate, then stir at 750rpm for 1 hour at room temperature to dissolve. Then, use an ultrafiltration membrane with a pore size of 0.1um to ultrafilter the solution and obtain pomegranate extract.

[0101] Comparative Example 3

[0102] A houttuynia cordata extract was prepared using the following steps:

[0103] Step 1: Crush 80g of Houttuynia cordata into powder and add it to 800g of 60% ethanol solution. Stir and extract at 60±5℃ for 3h, and centrifuge to obtain Houttuynia cordata extract. Concentrate the Houttuynia cordata extract to alcohol-free solution to obtain 160g of alcohol-free concentrate.

[0104] Step 2: Add 300g butanediol and 540g water to the alcohol-free concentrate, then stir at 750rpm for 1 hour at room temperature to dissolve. Then, use an ultrafiltration membrane with a pore size of 0.1um to ultrafilter the solution and obtain the houttuynia cordata extract.

[0105] Comparative Example 4

[0106] A roselle flower extract was prepared using the following steps:

[0107] Step 1: Crush 80g of hibiscus flowers into powder and add them to 800g of 60% ethanol solution. Stir and extract at 60±5℃ for 3h, and centrifuge to obtain hibiscus flower extract. Concentrate the hibiscus flower extract to alcohol-free solution to obtain 160g of alcohol-free concentrate.

[0108] Step 2: Add 300g butanediol and 540g water to the alcohol-free concentrate, then stir at 750rpm for 1 hour at room temperature to dissolve. Then, use an ultrafiltration membrane with a pore size of 0.1um to ultrafilter the solution and obtain the hibiscus flower extract.

[0109] Comparative Example 5

[0110] A composition is prepared by the following steps:

[0111] Step 1: Crush 80g of Houttuynia cordata into powder and add it to 800g of 60% ethanol solution. Stir and extract at 60±5℃ for 3h. Centrifuge to obtain Houttuynia cordata extract. Concentrate the Houttuynia cordata extract to alcohol-free solution to obtain 160g of alcohol-free concentrate A.

[0112] Step 2: Crush 40g of hibiscus flowers into powder and add them to 400g of 60% ethanol solution. Stir and extract at 60±5℃ for 3h. Centrifuge to obtain hibiscus flower extract. Concentrate the hibiscus flower extract to alcohol-free solution to obtain 80g of alcohol-free concentrate B.

[0113] Step 3: Mix alcohol-free concentrate A and alcohol-free concentrate B, add 300g butanediol and 460g water, and then stir at 750rpm for 1 hour at room temperature to dissolve. Then, use an ultrafiltration membrane with a pore size of 0.1um to ultrafilter the solution and obtain the composition.

[0114] Efficacy testing

[0115] I. 5α-Reductase Inhibition Rate

[0116] Test method:

[0117] 1. Experimental Objective and Principle

[0118] Oily skin has more active sebaceous glands, making the face feel greasy, difficult to clean, and shiny, which affects appearance.

[0119] It can also easily lead to some related skin diseases, such as acne vulgaris and seborrheic dermatitis. Given the relationship between 5α-reductase and sebaceous glands, the inhibitory effect of test samples on 5α-reductase is usually used to reflect the role of sebum regulation, so as to characterize the oil-controlling effect of test samples.

[0120] 2. Test Indicators

[0121] Criteria for determining 5α-reductase inhibition rate: If the 5α-reductase inhibition rate of the sample is higher than that of the negative control and there is a significant difference, then the test sample can be considered to have a certain oil control effect.

[0122] 3. Experimental Materials and Methods

[0123] Experimental materials: testosterone (≥98%), NADPH (90%), methanol (HPLC grade), finasteride (≥98%), buffer: PBS (pH=5.5).

[0124] 3.3 Test Methods

[0125] (1) Treatment of control materials and test samples

[0126] Sample group: Dilute with buffer to a concentration of 1% for the compositions of Examples 1-8 and Comparative Examples 1-5;

[0127] Positive control (finasteride, purity ≥98%): Dissolve in methanol first, then dilute with buffer to a finasteride test concentration of 400 nM;

[0128] Negative control: PBS buffer.

[0129] (2) Experimental operation procedures

[0130] A sample group, a positive control group, a negative control group, and a blank group were set up, with two replicates for each group. Different reagent solutions were added to the three groups, and the mixture was shaken well. Each group was then analyzed by HPLC to determine the testosterone content in each tube.

[0131] (3) Calculation formula

[0132] .

[0133] Examples 1-8 and Comparative Examples 1-5 were tested according to the above method, and the results are shown in Table 1.

[0134] Table 1. Results of 5α-reductase inhibition rate detection in Examples 1-8 and Comparative Examples 1-5

[0135] Group Average 5α-reductase inhibition rate % Example 1 70.29 Example 2 62.42 Example 3 67.26 Example 4 80.63 Example 5 64.15 Example 6 66.30 Example 7 63.25 Example 8 65.48 Comparative Example 1 47.29 Comparative Example 2 50.16 Comparative Example 3 39.27 Comparative Example 4 41.09 Comparative Example 5 43.18 Finasteride (400 nM) 66.24 Negative control (PBS) 6.07

[0136] According to the data in Table 1:

[0137] According to the data from Examples 1-3, the composition formed by combining oyster extract and pomegranate extract in this application has a high 5α-reductase inhibition rate and excellent oil control effect.

[0138] According to the data from Comparative Examples 1 and 2, at the same test concentration, the 5α-reductase inhibition rate of pomegranate extract is higher than that of oyster extract.

[0139] However, based on the data comparison of Examples 2 and 3, it can be seen that when oyster extract and pomegranate extract are combined, Example 3, with a higher pomegranate extract content, exhibits a weaker 5α-reductase inhibitory effect than Example 2, with a lower pomegranate extract content. Furthermore, by comparing the data of Example 1 and Comparative Examples 1 and 2, it can be inferred that this application produces a synergistic effect of enhancing the 5α-reductase inhibitory effect of the combination by combining oyster extract and pomegranate extract. However, this synergistic effect is based on oyster extract as the main ingredient to achieve better synergistic effect, rather than on pomegranate extract, which has a stronger 5α-reductase inhibitory effect.

[0140] A comparison of the data from Examples 4 and 1 shows that adding Houttuynia cordata extract and hibiscus flower extract to the combination of oyster extract and pomegranate extract can further enhance the 5α-reductase inhibitory effect of the composition. However, data from Examples 7 and 8 show that adding Houttuynia cordata extract or hibiscus flower extract alone to the combination of oyster extract and pomegranate extract does not effectively enhance the 5α-reductase inhibitory effect of the composition. The reasons for this may be as follows:

[0141] (1) Due to the addition of houttuynia cordata extract or roselle flower extract, it has an antagonistic effect with oyster extract and pomegranate extract, which have a synergistic effect, resulting in a decrease in the 5α-reductase inhibitory effect of the composition.

[0142] (2) Although the addition of houttuynia cordata extract or roselle flower extract produced a certain 5α-reductase inhibition effect, the concentration of oyster extract and pomegranate extract, which have a synergistic effect in the composition, decreased, resulting in a decrease in their own 5α-reductase inhibition rate. In other words, the addition of houttuynia cordata extract or roselle flower extract alone on the basis of oyster extract and pomegranate extract may only produce a simple effect superposition or a very weak further synergistic effect.

[0143] (3) The combination of Houttuynia cordata extract and roselle flower extract has a certain synergistic effect in enhancing the 5α-reductase inhibition effect of the composition. Therefore, the 5α-reductase inhibition rate of Example 4 is significantly higher than that of Example 1, Example 7 and Example 8.

[0144] To further verify the above possibilities, this application tested the 5α-reductase inhibitory effects of Houttuynia cordata extract and hibiscus flower extract on their own and the 5α-reductase inhibitory effects produced by their combination using Comparative Examples 3-5. It was found that the 5α-reductase inhibitory effects of Houttuynia cordata extract and hibiscus flower extract on their own were weaker than those of oyster extract. However, after Houttuynia cordata extract and hibiscus flower extract were combined, the 5α-reductase inhibitory effects of both showed a weak increase. In other words, the combination of the two has a weak synergistic effect.

[0145] Furthermore, based on the data from Example 7, Example 1, and Comparative Example 3, it can be inferred that: Example 7 added Houttuynia cordata extract alone to the combination of oyster extract and pomegranate extract. The concentration of the combination of oyster extract and pomegranate extract was only half that of Example 1. However, the 5α-reductase inhibition rate decreased significantly after adding Houttuynia cordata extract. It is possible that the addition of Houttuynia cordata extract only produced a simple superposition of effects.

[0146] Based on the data from Example 8, Example 1, and Comparative Example 4, it can be inferred that adding roselle flower extract alone to the combination of oyster extract and pomegranate extract is likely to produce a simple additive effect or a weak synergistic effect. As a result, when the concentration of oyster extract and pomegranate extract in the composition of Example 8 is 66% of that in the composition of Example 1, the 5α-reductase inhibition rate shows a more significant decrease.

[0147] Based on the data from Examples 7, 8 and Comparative Example 5, it is theoretically expected that the 5α-reductase inhibition rate of Example 4 should be weaker than that of Example 1. However, the 5α-reductase inhibition rate of Example 4 is significantly higher than that of Example 1. It is speculated that the addition of Houttuynia cordata extract and hibiscus flower extract to the combination of oyster extract and pomegranate extract may have produced a more significant synergistic effect in enhancing the 5α-reductase inhibition effect of the composition.

[0148] II. Inhibition rate of Propionibacterium acnes

[0149] Test method:

[0150] 1. Experimental Materials and Methods

[0151] 1.1 Instruments and equipment: Anaerobic incubator, vertical high-pressure steam sterilizer.

[0152] 1.2 Reagents: Phosphate buffer, reinforced Clostridium difficile medium (RCM), Propionibacterium acnes.

[0153] 2 Experimental Methods

[0154] 2.1 Sample preparation

[0155] Sample: Dilute with sterile water to a concentration of 5%;

[0156] Blank control: Phosphate buffer;

[0157] Control sample group: Propionibacterium acnes suspension + phosphate buffer.

[0158] 2.2 Test Procedure

[0159] Take a suspension of Propionibacterium acnes and add it to each sample solution and control solution, mix well, and start timing. After the specified reaction time, dilute appropriately. Take 2-3 dilutions and place the sample solution and control solution into two petri dishes respectively. Pour in Clostridium repens (RCM) medium and mix well. After solidification, invert the plates and incubate in an anaerobic incubator for 48 hours, then count the viable bacteria.

[0160] 3. Calculation Formula

[0161] Antibacterial rate = (AB) / A × 100%;

[0162] Where: A - average colony count of the control sample;

[0163] B - Average colony count of the tested sample.

[0164] 4. Data Analysis

[0165] Evaluation criteria: If the antibacterial rate is ≥50%, the product has antibacterial effect; if the antibacterial rate is ≥90%, the product has strong antibacterial effect.

[0166] The compositions of Examples 1-8 and Comparative Examples 1-5 were tested according to the above method, and the results are shown in Table 2.

[0167] Table 2. Results of Propionibacterium acnes inhibition rate of the compositions in Examples 1-8 and Comparative Examples 1-5

[0168] Group Inhibition rate of Propionibacterium acnes % Example 1 82.14 Example 2 85.00 Example 3 79.29 Example 4 ＞99.93 Example 5 83.57 Example 6 85.71 Example 7 93.21 Example 8 81.43 Comparative Example 1 57.14 Comparative Example 2 62.86 Comparative Example 3 67.86 Comparative Example 4 58.57 Comparative Example 5 62.86 control sample group /

[0169] According to the results in Table 2:

[0170] According to the data from Examples 1-3, the composition formed by combining oyster extract and pomegranate extract in this application has a high inhibition rate of Propionibacterium acnes and has excellent acne-removing effects.

[0171] According to the data from Comparative Examples 1 and 2, at the same test concentration, the inhibition rate of Propionibacterium acnes by pomegranate extract is higher than that of oyster extract. However, unlike the results in Table 2, the inhibition effect of Propionibacterium acnes in Example 2 is significantly higher than that in Examples 1 and 3, indicating that pomegranate extract plays a more important role in the inhibition of Propionibacterium acnes.

[0172] A comparison of the data from Example 4 and Example 1 shows that adding Houttuynia cordata extract and hibiscus flower extract to the combination of oyster extract and pomegranate extract can further enhance the inhibitory effect of the composition on Propionibacterium acnes. It should be noted that, due to the requirements of the testing standards, the result of Example 4 shows a greater than sign, and is not a constant value. This is a result of the testing standards.

[0173] Unlike the results in Table 2, the inhibition rates of Propionibacterium acnes in Examples 5 and 6 of this application are higher than those in Example 1. Based on the data from Comparative Examples 1 to 4, it can be inferred that the inhibitory effect of Propionibacterium acnes in this application is a synergistic effect produced by the combination of oyster extract, pomegranate extract, houttuynia cordata extract and hibiscus flower extract.

[0174] Furthermore, according to the data from Example 7, adding Houttuynia cordata extract alone to the combination of oyster extract and pomegranate extract can further enhance the inhibitory effect of the composition on Propionibacterium acnes. However, according to Example 8, adding hibiscus flower extract alone to the combination of oyster extract and pomegranate extract can weaken the inhibitory effect on Propionibacterium acnes. Based on the data from Comparative Examples 3 and 4, it can be inferred that adding Houttuynia cordata extract to the combination of oyster extract and pomegranate extract in Example 7 can produce a further synergistic effect in enhancing the inhibitory effect on Propionibacterium acnes. However, in Example 8, it is possible that the hibiscus flower extract has a simple additive effect or a weak synergistic effect with the oyster extract and pomegranate extract.

[0175] Comparing the data from Comparative Examples 5, 3, and 4, it can be seen that the combination of Houttuynia cordata extract and hibiscus flower extract did not significantly increase the inhibitory effect on Propionibacterium acnes, indicating that the combination of Houttuynia cordata extract and hibiscus flower extract only produces a simple synergistic effect. Furthermore, comparing the data from Examples 4, 7, and 8, it can be seen that the combination of oyster extract, pomegranate extract, Houttuynia cordata extract, and hibiscus flower extract can further significantly enhance the inhibitory effect of the composition on Propionibacterium acnes.

[0176] III. Stimulation Tests

[0177] Test method: Chicken embryo chorioallantoic membrane test

[0178] 1. Experimental Objective and Principle

[0179] The chorioallantoic membrane (CAM) test is an early and widely used in vitro method for assessing eye irritation. The CAM is a respiratory membrane surrounding the chicken embryo. This test utilizes the intact, clear, and transparent vascular system of the mid-stage chorioallantoic membrane in hatched chicken embryos. A certain amount of the test substance is directly exposed to the CAM, and after a period of time, changes in CAM toxicity indicators (such as hemorrhage, coagulation, and vascularization) are observed. These indicators reflect changes in the morphology, color, and permeability of blood vessels and vascular networks, as well as phenomena such as CAM protein denaturation and the degree of damage. These indicators are then combined to obtain a score used to assess the eye irritation of the test substance.

[0180] The purpose of this study is to test the ability of the test substance to cause toxic changes in the chorioallantoic membrane of chicken embryos and to evaluate the elements and processes of the potential eye irritation of the substance being evaluated.

[0181] 2. Experimental group treatments and reagents

[0182] Sample group: Dilute Example 4 with sterile water to prepare a 2% aqueous solution;

[0183] Negative control: 0.9% physiological saline;

[0184] Positive control: 1% sodium dodecyl sulfate (SDS) solution.

[0185] 3. Experimental Procedure

[0186] In this test, six chicken embryos were used in each group. The condition of the chorioallantoic membrane was recorded using a photographic device. A polytetrafluoroethylene (PTFE) resin ring was placed on the chorioallantoic membrane of the chicken embryo, and the image was taken. The test sample was added into the PTFE resin ring, and the time of sample addition was recorded. The air cell was then covered with a moistened plastic wrap, and the chicken embryos were transferred to a constant temperature and humidity incubator for cultivation. The degree of change in each toxic effect was observed.

[0187] 4 Result Calculation

[0188] The endpoint evaluation method was used for the experiment, and the endpoint evaluation (ES) was calculated. The results were retained to two decimal places. The score for each chicken embryo was the sum of the observed bleeding, coagulation and vascularization in each chicken embryo. The average of the mathematical sums obtained from ES-6 chicken embryos was used.

[0189] Calculate the average ES score using the following formula:

[0190] .

[0191] 5. Result Judgment Criteria

[0192] ES≤4, non-irritating;

[0193] 4 < ES ≤ 12, mild irritation;

[0194] 12 < ES < 16, moderately irritating;

[0195] ES≥16, highly irritating / corrosive.

[0196] Test Results

[0197] The sample of Example 4 was tested according to the above test method. ES=2.00, and the result was determined to be non-irritating.

[0198] Example 4: Chicken embryo results before and after use are as follows Figure 1 , Figure 2 As shown, where Figure 1This is a picture of the chicken embryo before use in Example 4. Figure 2 The image shows a chicken embryo after use in Example 4, demonstrating that the technical solution of the present invention is mild and non-irritating.

[0199] IV. Test of the Pore-Shrinking Efficacy of Cosmetics

[0200] 1 Overview

[0201] 1.1 Test Objective

[0202] This test verifies the pore-shrinking effect of the submitted sample by measuring the change in the percentage of pore area in healthy male and female subjects before and after using the sample.

[0203] 2 Test Plan

[0204] 2.1 Subject selection criteria

[0205] Participants were selected from a database, choosing healthy subjects who met the following inclusion and exclusion criteria:

[0206] 2.1.1 Selection Criteria

[0207] Select healthy Chinese men or women who meet the trial requirements;

[0208] Age 18-50 years old;

[0209] They can cooperate well with the test subjects and maintain a regular lifestyle during the research period.

[0210] 2.1.2 Exclusion Criteria

[0211] Anyone meeting any of the following conditions must be excluded from this study:

[0212] Those who have used antihistamines in the past week or immunosuppressants in the past month;

[0213] Those who have used any anti-inflammatory drugs on the test site within the past two months;

[0214] Subjects had clinically unhealed inflammatory skin conditions;

[0215] Patients with insulin-dependent diabetes mellitus;

[0216] Patients with asthma or other chronic respiratory diseases who are currently receiving treatment;

[0217] Those who have received anti-cancer chemotherapy within the past 6 months;

[0218] Patients with immunodeficiency or autoimmune diseases;

[0219] Breastfeeding or pregnant women;

[0220] Patients who have undergone bilateral mastectomy and bilateral axillary lymph node dissection;

[0221] The determination of test results is affected by scars, pigmentation, atrophy, port-wine stains, uneven skin color, folliculitis or other blemishes on the skin test site;

[0222] Researchers participating in other clinical trials;

[0223] Those who have participated in other clinical trials, including patch trials, within the past two months;

[0224] People with highly sensitive constitutions;

[0225] Those who are not participating voluntarily or are unable to complete the prescribed content as required by the experiment;

[0226] The researchers deemed the individual unsuitable to participate in this study.

[0227] 2.1.3 Exit Criteria

[0228] Patients may withdraw from a clinical study due to complications, adverse events, or other reasons during the study period, but the reason for withdrawal should be stated.

[0229] 2.2 Sample information, see Table 3:

[0230] Table 3 Sample Information Table

[0231] serial number Sample Name Testing items Testing basis 1 Acne-clearing, skin-purifying, and astringent multi-effect toner Cosmetic pore-minimizing efficacy test "Operating Instructions for Cosmetics' Pore-Shrinking Efficacy"

[0232] 2.3 Sample Usage Method

[0233] Application area: face.

[0234] Instructions for use: Apply D0 for the first time, test after 30 minutes, then wash off the sample; apply D3 for the second time, wash off, and test again on D7.

[0235] 2.4 Test Methods

[0236] 2.4.1 Instrument measurement parameters and related equipment are shown in Table 4:

[0237] Table 4. Instrument Measurement Parameters and Related Equipment

[0238] Serial Number Measurement parameters Instrument Name Test site Parameter Description 1 Pore ​​area percentage Skin image analysis software (image-Pro Plus v7.0) face A higher VISIA 7 image analysis value indicates a larger pore area ratio.

[0239] 2.4.2 Test procedure, see Table 5:

[0240] Table 5 Test Flowchart

[0241]

[0242] The sample containing Example 4 (with the component ratio shown in Table 6) was tested according to the above test method. The test results are shown in Table 7. It should be noted that the sample containing Example 4 was obtained by mixing all raw materials according to the formula in Table 6 and sterilizing at 85±3℃.

[0243] Table 6. Ingredients of Acne-Clearing, Skin-Cleansing, and Astringent Multi-Effect Toner

[0244] Raw material name Amount added (%) Example 4 1 1,2-Hexanediol 2 pure water 97

[0245] Table 7 Results of the test on the pore-shrinking efficacy of cosmetics

[0246]

[0247] After using the sample for 7 days, the pore area decreased by 6.96%, indicating that using the sample for 7 days had a certain pore-shrinking effect on the skin.

[0248] Application Example 1

[0249] An oil-controlling, acne-reducing, and pore-minimizing product is prepared according to the formula in Table 8. The preparation method is as follows:

[0250] Step 1: Pre-prepared phase: Dissolve phase B in advance and stir until homogeneous; mix phase C in advance and stir until homogeneous, set aside.

[0251] Step 2: Main pot: Add the pure water from phase A to the homogenizer, turn on the homogenizer, and while homogenizing, add the remaining raw materials from phase A sequentially, homogenizing at medium speed for 50 seconds. Stir and heat to 82±2℃, homogenize at medium speed for 2 minutes. Continue until no gel particles remain. Keep warm for 30 minutes, then stir and cool.

[0252] Step 3: Cool down to 50℃, add phases B and C in sequence, and stir well;

[0253] Step 4: Cool down to 45℃, add phase D, and stir well.

[0254] Table 8. Basic Oil-Control, Acne-Clearing, and Pore-Minimizing Product Formulas

[0255]

[0256] The embodiments presented herein are merely selected implementations based on combinations of all possible embodiments. The appended claims should not be limited to the embodiments described herein. Some numerical ranges used in the claims include sub-ranges within them, and variations within these ranges should also be covered by the appended claims.

Claims

1. A composition for controlling oil, removing acne, and shrinking pores, characterized in that, By weight, it includes oyster extract obtained from 1 to 8 parts of oyster meat, pomegranate extract obtained from 0.1 to 5 parts of pomegranate, houttuynia cordata extract obtained from 5 to 15 parts of houttuynia cordata and hibiscus flower extract obtained from 0.5 to 10 parts of hibiscus flower. The oil-controlling, acne-reducing, and pore-minimizing composition was obtained by the following method: Step a: Crush the oyster meat into powder and add it to 8-13 BV of water. Stir and extract at 80±5℃ for 2-4 hours, then centrifuge to obtain the oyster extract. Step b: Then, the oyster extract is concentrated to obtain a concentrate, wherein the mass ratio of the concentrate to the oyster extract is 1:5~11; Step c: After crushing the pomegranate into powder, add it to 10~20 BV of ethanol solution with a concentration of 60%, stir and extract at 60±5℃ for 2~3 hours, centrifuge to obtain pomegranate extract; concentrate the pomegranate extract until alcohol-free to obtain alcohol-free concentrate A; Step d: After crushing Houttuynia cordata into powder, add it to 10~20 BV of ethanol solution with a concentration of 60%, stir and extract at 60±5℃ for 2~3 hours, centrifuge to obtain Houttuynia cordata extract; concentrate the Houttuynia cordata extract to alcohol-free solution B. Step e: After crushing the hibiscus flowers into powder, add them to 10-20 BV of 60% ethanol solution and stir and extract at 60±5℃ for 2-3 hours. Centrifuge to obtain hibiscus flower extract; concentrate the hibiscus flower extract to alcohol-free solution C. Step f: Mix the concentrated solution, alcohol-free concentrated solution A, alcohol-free concentrated solution B, and alcohol-free concentrated solution C, then add polyol and water to obtain mixed solution B; the polyol content in mixed solution B is 20-50 wt%. Step g: Dissolve the mixed solution B by stirring and then ultrafiltration.

2. The oil-controlling, acne-removing, and pore-shrinking composition according to claim 1, characterized in that, The specific operation of stirring and dissolving is as follows: at room temperature, stir and dissolve at 600~750 rpm for 0.5~1 h; the specific operation of ultrafiltration is as follows: under an ultrafiltration pressure of 0.20~0.30 MPa, control the ultrafiltration temperature to not exceed 35℃, and use an ultrafiltration membrane pore size of 0.1~0.45 μm to obtain the filtrate.

3. The oil-controlling, acne-removing, and pore-shrinking composition according to claim 1, characterized in that, The specific operation of concentration is as follows: rotary evaporation is carried out at a temperature of 50~60℃, a pressure of -0.08~-0.1MPa, and a rotation speed of 40~60r / min.

4. Use of the oil-controlling, acne-removing, and pore-minimizing composition as described in any one of claims 1 to 3 to prepare skin care products.

5. A skincare product, characterized in that, Contains 0.01~30wt% of the oil-controlling, acne-reducing, and pore-shrinking composition as described in any one of claims 1~3.

Images