Cell sorting device for tumor cell detection and use method thereof

Abstract

The invention discloses a cell sorting device for tumor cell detection and a use method of the cell sorting device, and belongs to the technical field of cell sorting. The liquid level in a test tube is monitored in real time through an optical liquid level sensor, and a rotating seat drives the test tube to rotate by 90 degrees through a stand column and a fixed disc; the empty test tubes are transferred to one end of the optical liquid level sensor, uninterrupted liquid receiving work is carried out by adjusting the positions of the test tubes in the isolation frame, the test tubes filled with liquid do not need to be disassembled and assembled frequently, and all the test tubes in the isolation frame are moved out of the flow cytometry sorter by holding the grip and pulling the deflection frame downwards. One or more isolation frames are independently moved out of the flow cytometry sorter, and the rotating seats and the test tubes are all taken out of the isolation frames, so that batch detection work can be conveniently carried out on the test tubes, and meanwhile, the situation that all the test tubes in the base are exposed to pollute liquid in other test tubes is prevented; the influence on cell sorting and subsequent detection work is not easily caused.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cell sorting, in particular to a cell sorting device for tumor cell detection and a method of using the same. BACKGROUND

[0002] In tumor research and clinical diagnosis and treatment, efficient and accurate tumor cell detection and sorting technology is crucial for early diagnosis, efficacy evaluation and individualized treatment. Currently, common cell sorting technologies such as flow cytometry and magnetic bead cell sorting, flow cytometry is a precision instrument that can detect and sort small particles with multiple parameters quickly through optical principles, and is widely used in immunology, cell biology, genetic engineering and other fields. In the process of sorting tumor cells using flow cytometry, the cell sample is prepared into a single cell suspension, and specific cell components are labeled with fluorescent dyes. The cell suspension is added to the flow cytometry for automatic sorting. When the cell sorter sorts, different cells are dropped into different test tubes to complete the sorting of the cells. The detection personnel need to constantly replace the test tubes to collect the cell liquid. When replacing the test tubes, the entire test tube rack is usually removed from the flow cytometry, and then the test tubes with high liquid levels are replaced. The openings of all test tubes are exposed, which can easily contaminate the suspensions in other test tubes, affecting the accuracy of cell sorting and subsequent detection. SUMMARY

[0003] The present application aims to provide a cell sorting device for tumor cell detection and a method of using the same to solve the problem of replacing the test tubes, which usually requires removing the entire test tube rack from the flow cytometry, and then replacing the test tubes with high liquid levels. The openings of all test tubes are exposed, which can easily contaminate the suspensions in other test tubes, affecting the accuracy of cell sorting and subsequent detection.

[0004] To achieve the above-mentioned purpose, the present application provides the following technical solutions: A cell sorting device for tumor cell detection comprises a sorting box, a flow cell sorter is installed on the top of the sorting box, a control panel is provided on one side of the flow cell sorter, a base is fixedly installed inside the sorting box, three mounting grooves and three slides are respectively provided in the base, the mounting grooves pass through the front end and the top end of the base, the slides are provided at the bottom of the mounting grooves and the corresponding mounting grooves and slides are connected to each other, slides are respectively provided on both sides of the inner wall of the mounting groove, a card slot is provided at the position above the slide corresponding to the top of the inner wall of the front end of the base, an isolation frame is inserted in the mounting groove, the protrusions on both sides of the isolation frame slide in the slides on both sides, the bottom of the isolation frame is fixedly connected to a slide box, the slide box is slidably connected in the slide, and the bottom of the slide box is fixedly installed with The motor, the output shaft of the motor passes through the bottom of the isolation frame and is fixedly connected to the turntable, the turntable is clamped with a rotating seat, the rotating seat is provided with four arc grooves at equal intervals, the middle of the rotating seat is fixedly connected with a column, two fixed plates are clamped on the outside of the column, four sockets are provided on the fixed plate at equal intervals, and test tubes are inserted in the corresponding two sockets, and the bottom end of the test tube is overlapped with a support assembly, the bottom end of the support assembly is fixed in the arc groove, and the ends of the four support assemblies close to each other are connected to a limiting assembly, the limiting assembly passes through the rotating seat and the column and extends to the inside of the socket, the limiting assembly contacts the outer wall of the test tube, and a groove is provided on one side of the top front end of the isolation frame, a locking assembly is slidably installed in the groove, and the top end of the locking assembly is clamped in the slot.

[0005] As a further solution of the present invention, a rectangular groove is provided on the bottom of the rotating seat, the rectangular block on the turntable is clamped in the rectangular groove, and the turntable is embedded in the bottom of the rotating seat.

[0006] As a further solution of the present invention, three vertical poles are fixedly connected at equal intervals in the middle of the base, and optical liquid level sensors are installed on both sides of the top of the vertical poles. The detection ports of several optical liquid level sensors are respectively pointed to the top of the test tube, and alarms are installed on both sides of the flow cytometer corresponding to the positions above the isolation racks.

[0007] As a further solution of the present invention, the support assembly includes a support block, the inner wall of the support block is designed as an arc surface and overlaps with the bottom end of the test tube, a telescopic piston cylinder is fixed to the bottom of the support block, the bottom end of the telescopic piston cylinder is fixed in the arc groove, the outer wall of the telescopic piston cylinder is sleeved with a first spring, the lower part of the outside of the telescopic piston cylinder is connected to a first one-way valve, and the side of the outside of the telescopic piston cylinder away from the first one-way valve is connected to the bottom end of the limit assembly.

[0008] As a further scheme of the present application, the limiting assembly comprises an air guide pipe, the bottom end of the air guide pipe is communicated with the telescopic piston cylinder, two telescopic rods are connected to the outside of the air guide pipe, a second one-way valve is arranged at the connection between the telescopic rod and the air guide pipe, a clamping plate is fixedly connected to the telescopic end of the telescopic rod, the clamping plate is arc-shaped and in contact with the outer wall of the test tube, a connecting pipe is communicated between the fixed ends of the two telescopic rods, an exhaust valve pipe is communicated with the fixed end of the upper telescopic rod, and the top end of the exhaust valve pipe extends above the column.

[0009] As a further scheme of the present application, the locking assembly comprises a sliding block, the sliding block is slidingly connected in the groove, a clamping block is fixed on the sliding block, the clamping block penetrates through the groove and is clamped in the clamping groove, and the top of the clamping block is arc-shaped on one side of the test tube and is flat on the other side.

[0010] As a further scheme of the present application, the bottom of the sliding block is fixed with two sliding rods, the bottom end of the sliding rod penetrates through the bottom of the sliding block in the groove, a second spring is sleeved on the sliding rod, the top end of the second spring is fixed on the bottom of the sliding block and the bottom is fixed below the inner wall of the groove, an ear plate is fixed on one side of the sliding block, the ear plate penetrates through the groove and slidingly connects one side of the isolation frame, one end of the ear plate extending out of the isolation frame is rotatably connected with a deflection frame through a pin shaft, and the bottom of the deflection frame is fixed with a handle.

[0011] A use method of a cell sorting device for tumor cell detection, the use method comprising the following steps: In the process of sorting tumor cells, a cell sample is prepared into a single cell suspension, and a specific cell component is labeled with a fluorescent dye. Then, the cell suspension is added to the fluid chamber at the top of the flow cytometer. The flow cytometer works to make the cell suspension drop from below, and each drop of liquid contains a single cell. The liquid passes through the laser irradiation mechanism and the charge-coupled mechanism inside the flow cytometer, and the specific cells are guided into different test tubes by electric charge or sound waves. The optical liquid level sensor monitors the liquid level in the test tube in real time. When the liquid level of a test tube in the isolation frame reaches a preset height value, a signal is transmitted to the control panel. The control panel sends a signal to control the motor to work. The motor drives the turntable to rotate by ninety degrees. The rectangular block on the turntable drives the rotating seat to rotate. The rotating seat drives the test tube to rotate by ninety degrees through the column and the fixed disc. The test tube filled with liquid in the fixed disc deviates from the optical liquid level sensor, and the empty test tube is transferred to one end of the optical liquid level sensor. The same adjustment is made for the test tubes in other isolation frames. Therefore, the positions of the multiple test tubes in the isolation frame are adjusted continuously without frequent disassembly and assembly of the test tubes filled with liquid. When all the test tubes in the isolation frame are filled with liquid, the alarm corresponding to the isolation frame position on one side of the flow cytometer works, reminding the personnel to take out the test tube, by holding the handle and pulling the deflection frame downward, the deflection frame drives the slider to move downward in the groove through the ear plate, the slider drives the clamping block to disengage from the clamping groove, so as to release the locking state between the isolation frame and the base, and then pull the handle outward, the deflection frame rotates on one side of the ear plate through the pin shaft, at this time the deflection frame is in a relative horizontal state, so as to pull the isolation frame horizontally to move, the isolation frame drives the sliding box at the bottom to slide in the slide way, and the protruding parts on both sides of the isolation frame slide in the slide grooves on both sides, so that the isolation frame always maintains a horizontal movement state, improving the stability of adjusting the test tubes in the isolation frame, until all the test tubes in the isolation frame are removed from the base and the flow cytometer, by removing one or several isolation frames from the flow cytometer, preventing all the test tubes in the base from being exposed to the outside to contaminate the liquid in other test tubes; When one or several test tubes are taken out of the isolation frame, the exhaust valve pipe in the limiting assembly is opened, because the fixed ends of the two relative extension rods are connected by a connecting pipe, the gas in the extension rod is discharged through the exhaust valve pipe, when the internal gas pressure of the extension rod decreases, the extension end of the extension rod will contract and drive the clamping plate away from the test tube, that is, the locking state of the test tube is released, and the first spring supports the support block, during this process, the external gas enters the extension piston cylinder through the first one-way valve, the extension piston cylinder extends and drives the support block to lift the test tube upward, so as to take the test tube out of the two fixed discs, when installing the test tube, the exhaust valve pipe is closed, the test tube is inserted into the insertion hole in the fixed disc, and the bottom end of the test tube presses the support block, the support block presses the extension piston cylinder to increase the internal gas pressure of the extension piston cylinder, the first one-way valve blocks the gas in the extension piston cylinder from being discharged, and the gas in the extension piston cylinder enters the extension rod through the gas guide pipe, so that the extension rod extends and drives the clamping plate to clamp the test tube, because the second one-way valve is installed between the extension rod and the gas guide pipe, the gas in the extension rod cannot flow back to the extension piston cylinder through the gas guide pipe, ensuring that the clamping force of the clamping plate on the test tube, improving the stability of the test tube installation; When all the test tubes in the isolation frame are taken out and subsequent detection work is carried out, the fixed disc is lifted up, the fixed disc is driven upward by the column to make the rectangular block on the rotating disc disengage from the rectangular groove at the bottom of the rotating seat, the rotating seat and the test tubes are all taken out from the isolation frame, so that batch detection work can be carried out on the test tubes, and the detection efficiency is improved; when the rotating seat is installed, the rotating seat is inserted on the rotating disc, and the rectangular block on the rotating disc is inserted into the rectangular groove at the bottom of the rotating seat, so that the operation convenience is improved; when the isolation frame and the test tubes are pushed into the base for sorting work, the isolation frame is pushed, and the isolation frame slides in the slide way in the base; when the clamping block in the locking assembly contacts one side of the top of the base, the clamping block is pressed and moves downward due to the arc surface design on one side of the clamping block; when the test tubes in the isolation frame are completely located in the flow cytometer, the sliding block is supported by the elastic force of the second spring, the sliding block drives the slide rod and the clamping block to move upward, the clamping block is clamped into the clamping groove, and the purpose of locking the isolation frame and the base is achieved, and the next cell sorting work is carried out.

[0012] Compared with the prior art, the beneficial effects of the present application are: 1、The present application guides specific cells into different test tubes through the flow cytometer, and the optical liquid level sensor monitors the liquid level in the test tube in real time; when the liquid level of a test tube in the isolation frame reaches a preset height value, a signal is transmitted to the control panel, the control panel sends a signal to control the motor to work, the motor drives the rotating disc to rotate by 90 degrees, the rectangular block on the rotating disc drives the rotating seat to rotate, the rotating seat drives the test tube to rotate by 90 degrees through the column and the fixed disc, so that the test tube filled with liquid in the fixed disc deviates from the optical liquid level sensor, and the empty test tube is transferred to one end of the optical liquid level sensor; for the test tubes in other isolation frames, the positions of the test tubes are adjusted in the same way, so that uninterrupted liquid receiving work can be carried out by adjusting the positions of the multiple test tubes in the isolation frame, and the test tubes filled with liquid do not need to be frequently disassembled and assembled, the sorting efficiency is improved, and when all the test tubes in the isolation frame are filled with liquid, the alarm corresponding to the position of the isolation frame on one side of the flow cytometer works to remind the personnel to take out the test tubes; the handle is held and pulled downward to make the deflection frame drive the sliding block to move downward in the groove through the lug plate, the sliding block drives the clamping block to disengage from the clamping groove, so that the locking state between the isolation frame and the base is released, the handle is then pulled outward to keep the isolation frame in a horizontal motion state, the stability of the adjustment of the test tubes in the isolation frame is improved, and all the test tubes in the isolation frame are moved out of the base and the flow cytometer; by moving one or several isolation frames out of the flow cytometer, the fixed disc is lifted up, the fixed disc is driven upward by the column to make the rectangular block on the rotating disc disengage from the rectangular groove at the bottom of the rotating seat, the rotating seat and the test tubes are all taken out from the isolation frame, so that batch detection work can be carried out on the test tubes, the detection efficiency is improved, and all the test tubes in the base are prevented from being exposed to the outside to pollute the liquid in other test tubes, so that the cell sorting and subsequent detection work are not affected; 2, The application opens the exhaust valve pipe in the limiting assembly when taking out one or several test tubes in the isolation frame, because the fixed ends of the two opposite telescopic rods are connected with the connecting pipe, so that the gas in the telescopic rod is discharged through the exhaust valve pipe, when the internal pressure of the telescopic rod decreases, the telescopic end of the telescopic rod will shrink and drive the clamping plate away from the test tube, that is, the locking state of the test tube is released, and the first spring supports the supporting block, in this process, the external gas enters the telescopic piston cylinder through the first one-way valve, the telescopic piston cylinder is elongated to drive the supporting block to lift the test tube upward, so as to take the test tube out of the two fixed discs, when installing the test tube, the exhaust valve pipe is closed, the test tube is inserted into the insertion hole in the fixed disc, so that the bottom end of the test tube presses the supporting block, the supporting block presses the telescopic piston cylinder to increase the internal pressure of the telescopic piston cylinder, the first one-way valve blocks the discharge of the gas in the telescopic piston cylinder, and the gas in the telescopic piston cylinder enters the telescopic rod through the gas guide pipe, so that the telescopic rod is elongated and drives the clamping plate to clamp the test tube, because the second one-way valve is installed between the telescopic rod and the gas guide pipe, the gas in the telescopic rod cannot flow back to the telescopic piston cylinder through the gas guide pipe, so that the clamping force of the clamping plate on the test tube is ensured, the stability of the test tube installation is improved, and then the independent disassembly and assembly of the test tube are facilitated, and the operation convenience is improved. BRIEF DESCRIPTION OF DRAWINGS

[0013] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following will briefly introduce the drawings needed to be used in the description of the embodiments. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creating laborious work.

[0014] Figure 1 It is a three-dimensional structural schematic view of the present application; Figure 2 It is a three-dimensional structural schematic view of the base of the present application; Figure 3 It is a structural schematic view of the connection between the base and the isolation frame of the present application; Figure 4 It is a structural schematic view of the partial section of the base of the present application; Figure 5 It is a structural schematic view of the section of the isolation frame of the present application; Figure 6 It is a structural schematic view of the locking assembly of the present application; Figure 7 It is a structural schematic view of the connection between the stand and the fixed disc of the present application; Figure 8 It is a structural schematic view of the separation between the rotating disc and the rotating seat of the present application; Figure 9 It is a structural schematic view of the supporting assembly of the present application; Figure 10The structural schematic view of the limiting component of the present application; Figure 11 The structural schematic view of the limiting component of the present application Figure 10 The structural schematic view of the limiting component of the present application.

[0015] In the drawings, the components represented by each reference numeral are listed as follows: 1, sorting box; 2, flow cytometry sorter; 3, control panel; 4, base; 5, slide; 6, erection groove; 7, slide groove; 8, clamping groove; 9, vertical rod; 10, optical liquid level sensor; 11, isolation frame; 12, slide box; 13, motor; 14, turntable; 15, rotating seat; 16, arc groove; 17, rectangular groove; 18, stand column; 19, fixed disc; 20, jack; 21, test tube; 22, support assembly; 221, supporting block; 222, telescopic piston cylinder; 223, first spring; 224, first one-way valve; 23, limiting component; 231, air guide pipe; 232, second one-way valve; 233, telescopic rod; 234, clamping plate; 235, connecting pipe; 236, exhaust valve pipe; 24, recess; 25, locking assembly; 251, sliding block; 252, clamping block; 253, slide rod; 254, second spring; 255, ear plate; 256, deflection frame; 257, handle; 26, alarm. DETAILED DESCRIPTION

[0016] The technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the scope of protection of the present application.

[0017] Please refer to Figures 1-11 The present application provides a technical solution: The utility model provides a cell sorting device for tumor cell detection, including sorting box 1, the top of sorting box 1 is equipped with flow cytometry 2, one side of flow cytometry 2 is equipped with control panel 3, the inside fixed mounting of sorting box 1 is equipped with pedestal 4, three erecting grooves 6 and three slideways 5 are set up in pedestal 4 respectively, erecting groove 6 penetrates the front end and the top of pedestal 4, and the bottom of erecting groove 6 is provided with the slideway 5 that corresponds to erecting groove 6 and is communicated with each other, the both sides of the inner wall of erecting groove 6 are provided with the sliding slot 7 respectively, the top of the inner wall of the front end of pedestal 4 is provided with the clamping groove 8 corresponding to the position above slideway 5, the front end top of isolation frame 11 is inserted into erecting groove 6, the recess 24 is seted up in one side of the front end top of isolation frame 11, the locking assembly 25 is slidably installed in recess 24, and the top of locking assembly 25 is clamped in clamping groove 8; the protruding part of isolation frame 11 is slid in the sliding slot 7 of both sides, and the bottom of isolation frame 11 is fixedly connected with sliding box 12, and sliding box 12 is slidably connected in slideway 5; the bottom of sliding box 12 is fixedly installed with motor 13, the output shaft of motor 13 is fixedly connected with rotating disc 14 penetrating the bottom of isolation frame 11, rotating seat 15 is clamped on rotating disc 14, and four arc grooves 16 are seted up on rotating seat 15 at equal intervals; the middle part of rotating seat 15 is fixedly connected with stand 18, two fixed discs 19 are clamped on the outer stand 18, four insertion holes 20 are seted up on fixed disc 19 at equal intervals, and test tube 21 is inserted into corresponding two insertion holes 20, the sliding box 12 at the bottom of isolation frame 11 is slid in slideway 5, and the protruding part of isolation frame 11 is slid in the sliding slot 7 of both sides, so that isolation frame 11 always keeps horizontal motion state, and the stability of adjusting test tube 21 in isolation frame 11 is improved; As a further scheme of the utility model, the bottom of rotating seat 15 is provided with rectangular groove 17, and the rectangular block on rotating disc 14 is clamped in rectangular groove 17, and rotating disc 14 is embedded in the bottom of rotating seat 15; When working, the fixed disc 19 is lifted, the fixed disc 19 drives rotating seat 15 to move up through stand 18, the rectangular block on rotating disc 14 is separated from the rectangular groove 17 in the bottom of rotating seat 15, rotating seat 15 and test tube 21 are all taken out from isolation frame 11, so that batch detection work can be carried out on test tube 21, and the operation convenience is improved.

[0018] As a further scheme of the present application, the bottom end of the test tube 21 is overlapped with the supporting assembly 22, the bottom end of the supporting assembly 22 is fixed in the arc-shaped groove 16, the supporting assembly 22 comprises a supporting block 221, the inner wall of the supporting block 221 is arc-shaped and overlapped with the bottom end of the test tube 21, the bottom of the supporting block 221 is fixed with the telescopic end of a telescopic piston cylinder 222, the bottom end of the telescopic piston cylinder 222 is fixed in the arc-shaped groove 16, the telescopic end of the telescopic piston cylinder 222 is sleeved with a first spring 223, the lower part outside the telescopic piston cylinder 222 is connected with a first one-way valve 224, and the bottom end of the limiting assembly 23 is communicated with the side of the telescopic piston cylinder 222 away from the first one-way valve 224. In operation, the supporting block 221 is supported by the elastic force of the first spring 223, in this process, external gas enters the inside of the telescopic piston cylinder 222 through the first one-way valve 224, the first one-way valve 224 blocks the gas in the telescopic piston cylinder 222 from being discharged, and the telescopic piston cylinder 222 is elongated to drive the supporting block 221 to lift the test tube 21 upward, so as to take the test tube 21 out of the two fixed discs 19.

[0019] As a further scheme of the present application, the end of the four supporting assemblies 22 close to each other is connected with the limiting assembly 23, the limiting assembly 23 penetrates through the rotating seat 15 and the stand column 18 and extends into the insertion hole 20, and the limiting assembly 23 is in contact with the outer wall of the test tube 21. The limiting assembly 23 comprises a gas guide pipe 231, the bottom end of the gas guide pipe 231 is communicated with the telescopic piston cylinder 222, two telescopic rods 233 are connected with the outside of the gas guide pipe 231, a second one-way valve 232 is arranged at the connection between the telescopic rod 233 and the gas guide pipe 231, the telescopic end of the telescopic rod 233 extends through the fixed disc 19 and is fixedly connected with a clamping plate 234 in the insertion hole 20, the clamping plate 234 is arc-shaped and in contact with the outer wall of the test tube 21, a connecting pipe 235 is connected between the fixed ends of the two telescopic rods 233, an exhaust valve pipe 236 is connected to the fixed end of the upper telescopic rod 233 and extends above the stand column 18. The telescopic rod 233 is composed of a sleeve and a sliding rod, the sliding rod is sleeved in the sleeve, the sliding rod in the telescopic rod 233 is fixed with the clamping plate 234, and the sleeve in the telescopic rod 233 is communicated with the exhaust valve pipe 236, the connecting pipe 235 and the gas guide pipe 231. In operation, the gas in the telescopic piston cylinder 222 enters the inside of the telescopic rod 233 through the gas guide pipe 231, so that the telescopic rod 233 is elongated and drives the clamping plate 234 to clamp the test tube 21, and because the second one-way valve 232 is arranged between the telescopic rod 233 and the gas guide pipe 231, the gas in the telescopic rod 233 cannot flow back to the telescopic piston cylinder 222 through the gas guide pipe 231, so that the clamping force of the clamping plate 234 on the test tube 21 is ensured. When the two opposite telescopic rods 233 are locked, the gas in the telescopic rods 233 is discharged through the exhaust valve pipe 236, and the telescopic ends of the telescopic rods 233 are retracted to release the locking of the test tube 21.

[0020] As a further scheme of the present application, the locking assembly 25 comprises a sliding block 251 slidably connected in the groove 24, the sliding block 251 is fixed with a clamping block 252 penetrating the groove 24 and clamped in the clamping groove 8, the top of the clamping block 252 is designed as an arc surface on one side of the test tube 21 and a flat surface on the other side; due to the arc surface design of the clamping block 252, the clamping block 252 is pressed and moved downward to push the isolation frame 11 into the base 4.

[0021] As a further scheme of the present application, the bottom of the sliding block 251 is fixed with two sliding rods 253, the bottom ends of the sliding rods 253 penetrate the bottom of the sliding block 251 sliding in the groove 24, the sliding rods 253 are sleeved with a second spring 254, the top end of the second spring 254 is fixed on the bottom of the sliding block 251 and the bottom is fixed below the inner wall of the groove 24, one side of the sliding block 251 is fixed with an ear plate 255 penetrating the groove 24 and sliding on one side of the isolation frame 11, one end of the ear plate 255 extending out of the isolation frame 11 is rotatably connected with a deflection frame 256 through a pin shaft, the bottom of the deflection frame 256 is fixed with a handle 257; During operation, the sliding block 251 is supported by the elastic force of the second spring 254, so that the sliding block 251 drives the sliding rods 253 and the clamping block 252 to move upward, the clamping block 252 is clamped into the clamping groove 8, and the isolation frame 11 is locked with the base 4; By holding the handle 257 and pulling the deflection frame 256 downward, the deflection frame 256 drives the sliding block 251 to move downward in the groove 24 through the ear plate 255, the sliding block 251 drives the clamping block 252 to disengage from the clamping groove 8, so as to release the locking state between the isolation frame 11 and the base 4, and then the handle 257 is pulled outward, the deflection frame 256 is rotated on one side of the ear plate 255 through the pin shaft, at this time the deflection frame 256 is in a relative horizontal state, so as to horizontally pull the isolation frame 11 to move.

[0022] As a further scheme of the present application, the middle part of the base 4 is fixedly connected with three vertical rods 9 at equal intervals, the two sides of the top of the vertical rod 9 are respectively provided with an optical liquid level sensor 10, the detection ports of the plurality of optical liquid level sensors 10 are respectively directed to the top of the test tube 21, and the alarm 26 is installed on the positions above the plurality of isolation frames 11 on the two sides of the flow cytometer 2; When the optical liquid level sensor 10 is working, it monitors the liquid level in the test tube 21 in real time. When the liquid level in a test tube 21 in the isolation rack 11 reaches a preset height value, a signal is transmitted to the control panel 3, which sends a signal to control the motor 13 to work. The motor 13 drives the rotating disc 14 to rotate by 90 degrees. The rectangular block on the rotating disc 14 drives the rotating seat 15 to rotate. The rotating seat 15 drives the test tube 21 to rotate by 90 degrees through the vertical column 18 and the fixed disc 19. The test tube 21 filled with liquid in the fixed disc 19 deviates from the optical liquid level sensor 10, and the empty test tube 21 is transferred to one end of the optical liquid level sensor 10. When all the test tubes 21 in the isolation rack 11 are filled with liquid, the alarm 26 corresponding to the position of the isolation rack 11 on one side of the flow cytometer 2 works, reminding the personnel to take out the test tube 21.

[0023] A method for using a cell sorting device for tumor cell detection, the method comprising the following steps: When sorting tumor cells, a cell sample is prepared into a single-cell suspension, and specific cell components are labeled with fluorescent dyes. Then, the cell suspension is added to the fluid chamber at the top of the flow cytometer 2. The flow cytometer 2 works to make the cell suspension drop from below, with each drop containing a single cell. The liquid passes through the laser irradiation mechanism and the charge-coupled mechanism inside the flow cytometer 2, and specific cells are guided into different test tubes 21 by electric charge or sound waves. The optical liquid level sensor 10 monitors the liquid level in the test tube 21 in real time. When the liquid level in a test tube 21 in the isolation rack 11 reaches a preset height value, a signal is transmitted to the control panel 3, which sends a signal to control the motor 13 to work. The motor 13 drives the rotating disc 14 to rotate by 90 degrees. The rectangular block on the rotating disc 14 drives the rotating seat 15 to rotate. The rotating seat 15 drives the test tube 21 to rotate by 90 degrees through the vertical column 18 and the fixed disc 19. The test tube 21 filled with liquid in the fixed disc 19 deviates from the optical liquid level sensor 10, and the empty test tube 21 is transferred to one end of the optical liquid level sensor 10. The same adjustment is made for the test tubes 21 in other isolation racks 11. Thus, by adjusting the positions of the multiple test tubes 21 in the isolation rack 11, uninterrupted liquid receiving work is achieved without the need for frequent disassembly and assembly of the test tubes 21 filled with liquid. When all the test tubes 21 in the isolation frame 11 are filled with liquid, the alarm 26 corresponding to the position of the isolation frame 11 on one side of the flow cytometer 2 works to remind the personnel to take out the test tubes 21. By holding the handle 257 and pulling the deflection frame 256 downward, the deflection frame 256 drives the sliding block 251 to move downward in the groove 24 through the lug 255, and the clamping block 252 is driven by the sliding block 251 to disengage from the clamping groove 8, so as to release the locking state between the isolation frame 11 and the base 4. Then, the handle 257 is pulled outward, and the deflection frame 256 is rotated on one side of the lug 255 through the pin shaft, at this time, the deflection frame 256 is in a relative horizontal state, so as to facilitate horizontal pulling of the isolation frame 11 to move. The isolation frame 11 drives the sliding box 12 at the bottom to slide in the slide 5, and the protruding parts on both sides of the isolation frame 11 slide in the slide groove 7 on both sides, so that the isolation frame 11 always maintains a horizontal movement state, improving the stability of the adjustment of the test tubes 21 in the isolation frame 11. Until all the test tubes 21 in the isolation frame 11 are removed from the base 4 and the flow cytometer 2, by removing one or several isolation frames 11 from the flow cytometer 2, it is prevented that all the test tubes 21 in the base 4 are exposed to the outside to pollute the liquid in other test tubes 21; When one or several test tubes 21 are taken out from the isolation frame 11, the exhaust valve pipe 236 in the limiting assembly 23 is opened. Since the fixed ends of the two relative telescopic rods 233 are communicated with the connecting pipe 235, the gas in the telescopic rod 233 is discharged through the exhaust valve pipe 236. When the gas pressure in the telescopic rod 233 decreases, the telescopic end of the telescopic rod 233 will contract and drive the clamping plate 234 away from the test tube 21, that is, the locking state of the test tube 21 is released. The first spring 223 supports the supporting block 221. During this process, the external gas enters the telescopic piston cylinder 222 through the first one-way valve 224. The telescopic piston cylinder 222 is elongated to drive the supporting block 221 to lift the test tube 21 upward, so as to facilitate the removal of the test tube 21 from the two fixed discs 19. When the test tube 21 is installed, the exhaust valve pipe 236 is closed, the test tube 21 is inserted into the insertion hole 20 in the fixed disc 19, and the bottom end of the test tube 21 presses the supporting block 221. The supporting block 221 presses the telescopic piston cylinder 222 downward to increase the gas pressure in the telescopic piston cylinder 222. The first one-way valve 224 blocks the gas in the telescopic piston cylinder 222 from being discharged. The gas in the telescopic piston cylinder 222 enters the telescopic rod 233 through the gas guide pipe 231, so that the telescopic rod 233 is elongated and drives the clamping plate 234 to clamp the test tube 21. Since the second one-way valve 232 is installed between the telescopic rod 233 and the gas guide pipe 231, the gas in the telescopic rod 233 cannot flow back to the telescopic piston cylinder 222 through the gas guide pipe 231, so as to ensure the clamping force of the clamping plate 234 on the test tube 21 and improve the stability of the installation of the test tube 21; When all the test tubes 21 in the isolation frame 11 are taken out and subsequent detection work is carried out, the fixed disc 19 is lifted, the fixed disc 19 is driven by the stand 18 to move the rotating seat 15 upwards, the rectangular block on the rotating disc 14 is separated from the rectangular groove 17 at the bottom of the rotating seat 15, the rotating seat 15 and the test tubes 21 are all taken out from the isolation frame 11, so as to carry out batch detection work on the test tubes 21, improve the detection efficiency, when installing the rotating seat 15, the rotating seat 15 is inserted on the rotating disc 14, the rectangular block on the rotating disc 14 is inserted into the rectangular groove 17 at the bottom of the rotating seat 15, and the operation convenience is improved. When the isolation frame 11 and the test tubes 21 are pushed into the base 4 for sorting work, the isolation frame 11 is pushed to slide in the slide way 5 in the base 4, when the clamping block 252 in the locking assembly 25 contacts one side of the top of the base 4, due to the arc surface design of one side of the clamping block 252, the clamping block 252 is pressed and moves downwards, when the test tubes 21 in the isolation frame 11 are completely located in the flow cytometer 2, the sliding block 251 is supported by the elastic force of the second spring 254, the sliding block 251 drives the slide rod 253 and the clamping block 252 to move upwards, the clamping block 252 is clamped into the clamping groove 8, the purpose of locking the isolation frame 11 and the base 4 is achieved, and the next cell sorting work is carried out.

Claims

1. A cell sorting device for tumor cell detection, comprising a sorting box (1), characterized in that: A flow cell sorter (2) is installed on the top of the sorting box (1), and a control panel (3) is provided on one side of the flow cell sorter (2). A base (4) is fixedly installed inside the sorting box (1), and three mounting grooves (6) and three slideways (5) are respectively provided in the base (4). The mounting groove (6) passes through the front end and the top end of the base (4), and the slideway (5) is provided at the bottom of the mounting groove (6) and the corresponding mounting groove (6) and the slideway (5) are connected to each other. The inner wall of the mounting groove (6) is provided with A slide groove (7) is provided at the top of the inner wall of the front end of the base (4) at a position corresponding to the position above the slideway (5), an isolation frame (11) is inserted into the mounting groove (6), and the protruding parts on both sides of the isolation frame (11) slide in the slide grooves (7) on both sides, and the bottom of the isolation frame (11) is fixedly connected with a slide box (12), and the slide box (12) is slidably connected in the slideway (5), and a motor (13) is fixedly installed at the bottom of the slide box (12), and the output shaft of the motor (13) passes through the bottom of the isolation frame (11). A rotating disc (14) is fixedly connected to the rotating disc (14), a rotating seat (15) is clamped on the rotating disc (14), four arc-shaped grooves (16) are provided on the rotating seat (15) at equal intervals, a column (18) is fixedly connected to the middle of the rotating seat (15), two fixed discs (19) are clamped on the outside of the column (18), four jacks (20) are provided on the fixed disc (19) at equal intervals, test tubes (21) are inserted into the corresponding two jacks (20), the bottom end of the test tube (21) is overlapped with a support assembly (22), and the support assembly (2 2) is fixed in the arc groove (16), and one end of the four supporting components (22) close to each other is connected to the limiting component (23), the limiting component (23) passes through the rotating seat (15) and the column (18) and extends to the inside of the socket (20), the limiting component (23) contacts the outer wall of the test tube (21), and a groove (24) is provided on one side of the top of the front end of the isolation frame (11), and a locking component (25) is slidably installed in the groove (24), and the top end of the locking component (25) is clamped in the clamping groove (8).

2. A cell sorting device for tumor cell detection according to claim 1, characterized in that: A rectangular groove (17) is provided at the bottom of the rotating seat (15), the rectangular block on the rotating disk (14) is clamped in the rectangular groove (17), and the rotating disk (14) is embedded in the bottom of the rotating seat (15).

3. The cell sorting device for tumor cell detection according to claim 1, characterized in that: Three vertical poles (9) are fixedly connected at equal intervals in the middle of the base (4), and optical liquid level sensors (10) are respectively installed on both sides of the top of the vertical poles (9). The detection ports of several optical liquid level sensors (10) are respectively directed to the top of the test tube (21). Alarms (26) are respectively installed on both sides of the flow cell sorter (2) at positions above several isolation racks (11).

4. The cell sorting device for tumor cell detection according to claim 1, characterized in that: The support assembly (22) includes a support block (221), the inner wall of the support block (221) is designed as an arc surface and overlaps the bottom end of the test tube (21), a telescopic piston cylinder (222) is fixed to the bottom of the support block (221), the bottom end of the telescopic piston cylinder (222) is fixed in the arc groove (16), the outer wall of the telescopic piston cylinder (222) is sleeved with a first spring (223), the lower part of the outside of the telescopic piston cylinder (222) is connected to a first one-way valve (224), and the side of the outside of the telescopic piston cylinder (222) away from the first one-way valve (224) is connected to the bottom end of the limit assembly (23).

5. The cell sorting device for tumor cell detection according to claim 4, characterized in that: The limiting assembly (23) includes an air guide tube (231), the bottom end of the air guide tube (231) is connected to the telescopic piston cylinder (222), the air guide tube (231) is externally connected to two telescopic rods (233), a second one-way valve (232) is installed at the connection between the telescopic rod (233) and the air guide tube (231), the telescopic end of the telescopic rod (233) is fixedly connected to a splint (234), the splint (234) is arc-shaped and contacts the outer wall of the test tube (21), a connecting tube (235) is connected between the fixed ends of the two telescopic rods (233), and the fixed end of the upper telescopic rod (233) is connected to an exhaust valve tube (236), the top end of the exhaust valve tube (236) extends to the top of the column (18).

6. The cell sorting device for tumor cell detection according to claim 1, characterized in that: The locking assembly (25) includes a slider (251), the slider (251) is slidably connected in the groove (24), a clamping block (252) is fixed on the slider (251), the clamping block (252) passes through the groove (24) and is clamped in the clamping slot (8), and the top of the clamping block (252) is designed with a curved surface on one side close to the test tube (21) and a flat surface on the other side.

7. A cell sorting device for tumor cell detection according to claim 6, characterized in that: Two slide bars (253) are fixed to the bottom of the slider (251), the bottom ends of the slide bars (253) pass through the bottom of the groove (24), and the outer sleeve of the slide bars (253) is connected to a second spring (254). The top end of the second spring (254) is fixed to the bottom of the slider (251) and the bottom is fixed below the inner wall of the groove (24). An ear plate (255) is fixed to one side of the slider (251), and the ear plate (255) passes through the groove (24) and slides on one side of the isolation frame (11). One end of the ear plate (255) extending out of the isolation frame (11) is rotatably connected to a deflection frame (256) through a pin shaft, and a handle (257) is fixed to the bottom of the deflection frame (256).

8. A method for using a cell sorting device for tumor cell detection, according to any one of claims 1 to 7, characterized in that: The method of use comprises the following steps: When sorting tumor cells, the cell sample is made into a single cell suspension and specific cell components are labeled with fluorescent dyes. Then, the cell suspension is added to the fluid chamber at the top of the flow cytometer (2). The flow cytometer (2) works to make the cell suspension drip from the bottom. Each drop of liquid contains a single cell. The liquid passes through the laser irradiation mechanism and charge coupling mechanism inside the flow cytometer (2), and guides specific cells into different test tubes (21) through charge or sound waves. The optical liquid level sensor (10) monitors the liquid level in the test tube (21) in real time. When the liquid level of a test tube (21) in the isolation rack (11) reaches the preset height value, the signal is transmitted to the control panel (3), and the control panel (3) generates a signal. The signal is sent to control the motor (13) to work, and the motor (13) drives the turntable (14) to rotate ninety degrees. The rectangular block on the turntable (14) drives the rotating seat (15) to rotate. The rotating seat (15) drives the test tube (21) to rotate ninety degrees through the column (18) and the fixed plate (19), so that the test tube (21) filled with liquid in the fixed plate (19) deviates from the optical liquid level sensor (10), and the empty test tube (21) is transferred to one end of the optical liquid level sensor (10). The test tubes (21) in other isolation racks (11) are adjusted in the same way. Therefore, by adjusting the positions of multiple test tubes (21) in the isolation rack (11), uninterrupted liquid connection work is performed, and there is no need to frequently disassemble and assemble the test tubes (21) filled with liquid; When all the test tubes (21) in the isolation rack (11) are filled with liquid, the alarm (26) at the position of the isolation rack (11) on one side of the flow cell sorter (2) works to remind the personnel to take out the test tubes (21). By holding the handle (257) and pulling the deflection rack (256) downward, the deflection rack (256) drives the slider (251) to move downward inside the groove (24) through the ear plate (255), so that the slider (251) drives the block (252) to disengage from the slot (8), thereby releasing the locking state between the isolation rack (11) and the base (4). Then, the handle (257) is pulled outward to rotate the deflection rack (256) on one side of the ear plate (255) through the pin. At this time, the deflection rack (256) The isolation rack (11) is in a relatively horizontal state so as to facilitate the horizontal pulling of the isolation rack (11) for movement. The isolation rack (11) drives the slide box (12) at the bottom to slide in the slideway (5). At the same time, the protruding parts on both sides of the isolation rack (11) slide in the slide grooves (7) on both sides, so that the isolation rack (11) always maintains a horizontal movement state, thereby improving the stability of adjusting the test tubes (21) in the isolation rack (11) until all the test tubes (21) in the isolation rack (11) are moved out of the base (4) and the flow cell sorter (2). By moving one or more isolation racks (11) out of the flow cell sorter (2) separately, all the test tubes (21) inside the base (4) are prevented from being exposed to the outside and causing contamination to the liquid inside other test tubes (21); When one or more test tubes (21) are taken out from the isolation rack (11), the exhaust valve tube (236) in the limit assembly (23) is opened. Since the fixed ends of the two opposite telescopic rods (233) are connected with a connecting tube (235), the internal gas of the telescopic rod (233) is discharged through the exhaust valve tube (236). When the internal air pressure of the telescopic rod (233) decreases, the telescopic end of the telescopic rod (233) contracts and drives the clamping plate (234) away from the test tube (21), that is, the locking state of the test tube (21) is released, and the elastic force of the first spring (223) is used to support the support block (221). During this process, the external gas enters the interior of the telescopic piston cylinder (222) through the first one-way valve (224). The telescopic piston cylinder (222) is extended to drive the support block (221) to push the test tube (21) upward, so that the test tube (21) can be removed from the two fixed plates (19). When the test tube (21) is installed, ), the exhaust valve tube (236) is closed, and the test tube (21) is inserted into the socket (20) in the fixed plate (19), so that the bottom end of the test tube (21) squeezes the support block (221), and the support block (221) presses down the telescopic piston cylinder (222) to increase the internal air pressure, and the first one-way valve (224) blocks the internal air of the telescopic piston cylinder (222) from being discharged, and the internal air of the telescopic piston cylinder (222) enters the telescopic rod (222) through the air guide tube (231). 233), the telescopic rod (233) is extended and drives the clamping plate (234) to clamp the test tube (21). Since the second one-way valve (232) is installed between the telescopic rod (233) and the air guide tube (231), the gas inside the telescopic rod (233) cannot flow back into the telescopic piston cylinder (222) through the air guide tube (231), thereby ensuring the clamping force applied by the clamping plate (234) to the test tube (21) and improving the stability of the installation of the test tube (21); When all the test tubes (21) in the isolation rack (11) are taken out and subsequent testing is carried out, the fixed plate (19) is lifted up, so that the fixed plate (19) drives the rotating seat (15) to move up through the column (18), so that the rectangular block on the turntable (14) is separated from the rectangular groove (17) at the bottom of the rotating seat (15), and the rotating seat (15) and the test tubes (21) are all taken out from the isolation rack (11), so as to facilitate batch testing of the test tubes (21) and improve the testing efficiency. When installing the rotating seat (15), the rotating seat (15) is plugged into the turntable (14) so ​​that the rectangular block on the turntable (14) is inserted into the rectangular groove (17) at the bottom of the rotating seat (15), thereby improving the convenience of operation. Then, the isolation rack (11) and the test tubes (21) are pushed into the base (4). ) when sorting is performed inside the isolation rack (11), the isolation rack (11) is pushed to slide in the slideway (5) in the base (4). When the block (252) in the locking assembly (25) contacts one side of the top of the base (4), the block (252) is compressed and moves downward because one side of the block (252) is designed as an arc surface. When the test tube (21) in the isolation rack (11) is completely located inside the flow cell sorter (2), the slider (251) is supported by the elastic force of the second spring (254), so that the slider (251) drives the slide bar (253) and the block (252) to move upward, so that the block (252) is stuck in the slot (8), thereby achieving the purpose of locking the isolation rack (11) and the base (4) and performing the next cell sorting work.

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