Cell sorter

Abstract

The utility model discloses a cell sorter, which belongs to the technical field of magnetic bead type cell sorting and comprises a box body, and the middle of the upper end of the box body is hinged with a baffle plate; the pull rod is fixedly mounted at the upper end of the baffle; the lower end of the box body is fixedly connected with a baseplate; a central control box is fixedly mounted at the outer end of the box body; the magnetic block is fixedly mounted on the inner wall of the left side in the box body; the two partition plates are symmetrically and fixedly installed on the front side and the rear side in the box body, the interior of the box body is divided into a containing bin and a magnetic field separation bin, and a circulating material moving mechanism is installed on the bottom plate. The circulating material moving mechanism comprises a material moving assembly, a synchronous reverse driving assembly and a bearing assembly, and the central control box is electrically connected with the synchronous reverse driving assembly. Through cooperation of the material moving assembly, the synchronous reverse driving assembly and the bearing assembly, magnetic beads and target cells are combined more completely, and the cell sorting efficiency is improved; the interior of the box body is divided into a plurality of chambers by the partition plates, so that test tubes which are not subjected to cell sorting and test tubes subjected to cell sorting in the box body are separated.

Description

TECHNICAL FIELD

[0001] The utility model relates to the magnetic bead formula cell sorting technical field, concretely relates to a cell sorter. BACKGROUND

[0002] Magnetic bead formula cell sorting is based on the principle that cell surface antigen combines with specific monoclonal antibody connected with magnetic bead. In the additional magnetic field, the cell connected with magnetic bead through antibody is adsorbed and stays in the magnetic field, and the cell without the surface antigen cannot combine with the specific monoclonal antibody connected with magnetic bead and has no magnetism, does not stay in the magnetic field, so that the cell can be separated.

[0003] Chinese patent CN216614691U discloses a kind of device for sorting cell using magnetic bead, including base, the upper end of the base is symmetrically equipped with two L-shaped placement plate, two L-shaped placement plate are fixedly connected with limiting plate between them, the upper end of two L-shaped placement plate is equipped with a plurality of placement holes, the limiting plate is equipped with placement slot corresponding to a plurality of placement holes, each placement slot is equipped with test tube, the upper end of test tube passes through corresponding placement hole, the opposite side of two L-shaped placement plate is equipped with mounting slot, the front and rear inner wall of each mounting slot is equipped with damping sliding slot, each mounting slot is equipped with electromagnet inside. However, the device still has the following problems:

[0004] When cell and magnetic bead are mixed together, manual mode is generally used to take and place, but the temperature of artificial hand can affect the temperature of sample tube, and hand can have bacteria, virus or other contaminants, these substances can enter sample tube, affect experimental result, and the cell purity obtained by the sorting device is not very high after once sorting, the quality of cell sorting is reduced.

[0005] Therefore, the utility model designs a kind of cell sorter to solve above-mentioned problem. UTILITY MODEL CONTENT

[0006] In view of the above-mentioned shortcomings of prior art, the utility model provides a kind of cell sorter.

[0007] To achieve the above object, the utility model is realized by the following technical scheme:

[0008] A kind of cell sorter, including box body,

[0009] Box body upper end middle part is hinged to baffle;

[0010] Pull rod is fixedly installed in the upper end of baffle;

[0011] Box body lower end is fixedly connected with bottom plate;

[0012] The outer end of box body is fixedly installed with central control box;

[0013] The magnetic block is fixedly installed on the left inner wall of the box body;

[0014] Two partitions are symmetrically fixedly installed on the front and back inner sides of the box body, so as to divide the box body into a storage chamber and a magnetic field separation chamber, and a through slot is formed between the two partitions, so that the two chambers are communicated with each other; the storage chamber is used for storing the cell solution test tube to which the antibody labeled magnetic beads are added, and the magnetic field separation chamber is used for separating the cells in the test tube which have been adsorbed with the magnetic beads from other cells;

[0015] The bottom plate is provided with a circulating material moving mechanism for driving the cell sorting of the test tube to which the cell solution is added;

[0016] The circulating material moving mechanism comprises a material moving assembly, a synchronous reverse driving assembly and a bearing assembly, the bottom plate is provided at the lower end thereof with the synchronous reverse driving assembly for controlling the movement of the material moving assembly, the box body is provided at the lower inner end thereof with the material moving assembly for controlling the test tube to change the chamber, and the material moving assembly and the synchronous reverse driving assembly are connected; the bearing assembly is installed on the material moving assembly; and the central control box is electrically connected with the synchronous reverse driving assembly;

[0017] Further, the magnetic block is U-shaped;

[0018] Further, the synchronous reverse driving assembly comprises a motor, a driving gear, a driven gear and a transmission assembly, the motor is fixedly installed on the lower inner end of the box body, the motor is connected with the transmission assembly, the driving gear is rotatably connected on the left lower end of the bottom plate, and the driven gear is rotatably connected on the right lower end of the bottom plate; the motor and the driving gear are connected with the transmission assembly; and the motor is electrically connected with the central control box;

[0019] Further, the transmission assembly comprises a worm gear and a worm, the lower end of the driving gear is fixedly connected with the worm gear, and the output end of the motor is fixedly connected with one end of the worm; and the worm gear and the worm are meshingly connected;

[0020] Further, the material moving assembly comprises a left rotating disc, a right rotating disc, a U-shaped limiting slot and an eight-shaped sliding slot, the left rotating disc is rotatably installed on the left upper end of the bottom plate and is fixedly connected with the driving gear, and the right rotating disc is rotatably installed on the right upper end of the bottom plate and is fixedly connected with the driven gear; a plurality of U-shaped limiting slots are formed on the left rotating disc and the right rotating disc; and the eight-shaped sliding slot is formed on the upper end of the bottom plate;

[0021] Further, the U-shaped limiting slots are uniformly distributed on the left rotating disc and the right rotating disc at equal intervals in a circumferential direction;

[0022] Further, the bearing assembly comprises a sleeve, a snap ring and an oval sliding block, the snap ring is fixedly installed at the middle outer end of the sleeve, and the bottom of the sleeve is fixedly installed with the oval sliding block; the snap ring is in limiting sliding connection with the U-shaped limiting groove, and the oval sliding block is in limiting sliding connection with the splayed sliding groove; the test tube is inserted with the sleeve;

[0023] Further, the inner diameter of the sleeve is larger than the outer diameter of the test tube;

[0024] Further, the middle part of the splayed sliding groove is the junction position of the storage chamber and the magnetic field separation chamber;

[0025] Further, the baffle is provided with an observation window made of transparent material.

[0026] Compared with the prior art, the utility model has the advantages that: 1. Through the cooperation of the material moving assembly, the synchronous reverse driving assembly and the bearing assembly, the magnetic beads are combined with the target cells more completely, so that the finally sorted cell liquid is closer to the requirement, and the efficiency of cell sorting is improved;

[0027] 2. The partition plate divides the inside of the box body into multiple chambers, so that the cell sorting test tubes and the cell sorted test tubes are separated, the practicality of the disinfection device is improved, and use is facilitated;

[0028] 3. Through the baffle arranged on the box body, the pollutants are effectively prevented from entering the test tube, and the quality of cell sorting is ensured. BRIEF DESCRIPTION OF DRAWINGS

[0029] In order to more clearly illustrate the technical scheme in the embodiments of the utility model or the prior art, the following will briefly introduce the drawings needed to be used in the embodiment or the prior art description. Obviously, the drawings in the following description are only some embodiments of the utility model, and for those skilled in the art, other drawings can also be obtained according to these drawings without creative labor.

[0030] Figure 1 It is a perspective view of the cell sorting instrument of the utility model Figure 1 ;

[0031] Figure 2 It is a front view of the cell sorting instrument of the utility model

[0032] Figure 3 It is a perspective view of the cell sorting instrument of the utility model Figure 2 ;

[0033] Figure 4 It is a perspective view along the A-A direction of Figure 2 ;

[0034] Figure 5for cutting along the B-B direction of the figure to show a partial perspective view thereof; Figure 2 for cutting along the B-B direction of the figure to show a partial perspective view thereof;

[0035] Figure 6 for cutting along the B-B direction of the figure to show a partial perspective view thereof;

[0036] The reference numerals in the figures respectively represent:

[0037] 1, box body; 11, placement chamber; 12, magnetic field separation chamber; 2, baffle; 3, pull rod; 4, partition; 5, circulating material moving mechanism; 51, material moving assembly; 511, left rotating disc; 512, right rotating disc; 513, U-shaped limiting groove; 514, eight-character sliding groove; 52, synchronous reverse driving assembly; 521, motor; 522, driving gear; 523, driven gear; 524, worm wheel; 525, worm; 53, bearing assembly; 531, sleeve; 532, snap ring; 533, oval sliding block; 6, magnetic block; 7, bottom plate; 8, test tube; 9, central control box. DETAILED DESCRIPTION

[0038] In order to make the purpose, technical scheme and advantages of the embodiments of the present application more clear, the technical scheme in the embodiments of the present application will be described clearly and completely below in combination with the drawings in the embodiments of the present application. Obviously, the described embodiments are some embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all the other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0039] In the following description, "left", "right", "front", "rear", "upper", and "lower" are oriented in the perspective view.

[0040] Embodiment one: in some embodiments, referring to the Figures 1-6 A cell sorter, comprising a box body 1,

[0041] The upper end of the box body 1 is hingedly connected with a baffle 2;

[0042] A pull rod 3 is fixedly installed at the upper end of the baffle 2; the baffle 2 is provided with an observation window of transparent material for medical staff to observe the sorting progress inside the sorter;

[0043] The box body 1 is fixedly connected with a bottom plate 7 at the lower end;

[0044] The box body 1 is fixedly installed with a central control box 9 at the outer end;

[0045] A magnetic block 6 is fixedly installed at the left inner wall inside the box body 1; the magnetic block 6 is U-shaped;

[0046] Two partition plates 4 are symmetrically fixedly installed at the inside front and back of the box body 1, and divide the inside of the box body 1 into a placing chamber 11 and a magnetic field separation chamber 12, and a through slot is formed between the two partition plates 4, so that the two chambers are communicated with each other; wherein the placing chamber 11 is used for storing the cell solution test tube 8 to which the antibody marked magnetic beads are added, and the magnetic field separation chamber 12 is used for separating the cells in the test tube 8 which have been adsorbed with the magnetic beads from other cells;

[0047] A circulating material moving mechanism 5 for moving the test tube 8 to which the cell solution is added to carry out cell sorting is installed on the bottom plate 7;

[0048] The circulating material moving mechanism 5 comprises a material moving assembly 51, a synchronous reverse driving assembly 52 and a bearing assembly 53, the synchronous reverse driving assembly 52 for controlling the movement of the material moving assembly 51 is installed at the lower end of the bottom plate 7, the material moving assembly 51 for controlling the test tube 8 to change the chamber is installed at the lower end of the inside of the box body 1, and the material moving assembly 51 and the synchronous reverse driving assembly 52 are connected; the bearing assembly 53 is installed on the material moving assembly 51; the central control box 9 is electrically connected with the synchronous reverse driving assembly 52;

[0049] A plurality of bearing assemblies 53 for placing the test tube 8 are installed on the material moving assembly 51;

[0050] In the utility model, the medical staff adds the magnetic beads marked with the antibody into the test tube 8 containing the cell suspension, then pulls the baffle 2 through the pull rod 3 and places the test tube 8 into the bearing assembly 53 in the placing chamber 11, and presses the central control box 9 arranged outside the box body 1 to make the synchronous reverse driving assembly 52 work and drive the material moving assembly 51 to move, the material moving assembly 51 works to make the bearing assembly 53 in the placing chamber 11 move to the magnetic field separation chamber 12, when the bearing assembly 53 enters the magnetic field separation chamber 12, the magnetic block 6 attracts the cells connected with the magnetic beads, and the cells not connected with the magnetic beads are suspended in the upper layer of the solution in the test tube 8; after the test tube 8 moves in the magnetic field separation chamber 12 for a period of time, the medical staff sucks the cells not adsorbed with the magnetic beads in the upper layer of the test tube 8, then the synchronous reverse driving assembly 52 works again to drive the material moving assembly 51 to move, so that the test tube 8 after cell sorting is moved to the placing chamber 11 again;

[0051] In the utility model, through the cooperation of the material moving assembly 51, the synchronous reverse driving assembly 52 and the bearing assembly 53, the combination of the magnetic beads and the target cells is more complete, so that the cell solution after final sorting is more close to the requirement, and the efficiency of cell sorting is improved; the partition plate 4 divides the inside of the box body 1 into a plurality of chambers, so that the test tube 8 not subjected to cell sorting and the test tube 8 after cell sorting are separated in the inside of the box body 1, the practicality of the disinfection device is improved, and the disinfection device is convenient to use; through the baffle 2 arranged on the box body 1, the pollutants are effectively prevented from entering the test tube 8, and the quality of cell sorting is ensured.

[0052] In some embodiments, as shown in FIG. 1, the reverse driving assembly 52 includes a motor 521, a driving gear 522, a driven gear 523, a worm wheel 524 and a worm 525. The motor 521 is fixedly installed on the inner wall of the lower end of the box body 1. The driving gear 522 is rotatably connected to the left side of the lower end of the bottom plate 7. The driven gear 523 is rotatably connected to the right side of the lower end of the bottom plate 7. The driving gear 522 is in meshing connection with the driven gear 523. The lower end of the driving gear 522 is fixedly connected with the worm wheel 524. The output end of the motor 521 is fixedly connected with one end of the worm 525. The worm wheel 524 and the worm 525 are in meshing connection. The motor 521 is electrically connected with the central control box 9. Figures 4-6

[0053] The material moving assembly 51 includes a left rotating disc 511, a right rotating disc 512, a U-shaped limiting groove 513 and an eight-shaped sliding groove 514. The left rotating disc 511 is rotatably installed on the left side of the upper end of the bottom plate 7 and is fixedly connected with the driving gear 522. The right rotating disc 512 is rotatably installed on the right side of the upper end of the bottom plate 7 and is fixedly connected with the driven gear 523. A plurality of U-shaped limiting grooves 513 are formed in the left rotating disc 511 and the right rotating disc 512. The U-shaped limiting grooves 513 are evenly distributed in a circumferential direction of the left rotating disc 511 and the right rotating disc 512. The eight-shaped sliding groove 514 is formed in the upper end of the bottom plate 7. The middle part of the eight-shaped sliding groove 514 is the boundary position between the storage chamber 11 and the magnetic field separation chamber 12.

[0054] The bearing assembly 53 includes a sleeve 531, a clamping ring 532 and an oval sliding block 533. The clamping ring 532 is fixedly installed on the outer end of the middle part of the sleeve 531. The oval sliding block 533 is fixedly installed on the bottom of the sleeve 531. The clamping ring 532 is in limiting sliding connection with the U-shaped limiting groove 513. The oval sliding block 533 is in limiting sliding connection with the eight-shaped sliding groove 514. The inner diameter of the sleeve 531 is greater than the outer diameter of the test tube 8. The test tube 8 is inserted into the sleeve 531.

[0055] In the utility model, the medical staff inserts the test tube 8 containing the cell solution into the inside of the sleeve 531. Then the motor 521 is started by the central control box 9. The motor 521 drives the worm 525 to rotate. The worm 525 drives the worm wheel 524 to rotate. The driving gear 522 rotates together with the worm wheel 524. The driving gear 522 drives the driven gear 523 to rotate in the opposite direction. In this process, the driving gear 522 drives the left rotating disc 511 to rotate. The driven gear 523 drives the right rotating disc 512 to rotate.

[0056] ​When the elliptical sliding block 533 in the placing chamber 11 slides to the middle of the splayed sliding groove 514, the elliptical sliding block 533 drives the clasp 532 to slide from the U-shaped limiting groove 513 on the right rotating disc 512 into the U-shaped limiting groove 513 on the left rotating disc 511 in the magnetic field separation chamber 12; the driving gear 522 continues to rotate, driving the left rotating disc 511 to rotate; when the sleeve 531 completely enters the magnetic field separation chamber 12, the cells combined with the magnetic beads in the test tube 8 are attracted by the magnetic block 6 in the magnetic field separation chamber 12 at this time, and the cells not combined with the magnetic beads are suspended in the upper layer of the cell solution in the test tube 8;

[0057] When the left rotating disc 511 drives the clasp 532 to rotate a whole circle and slide to the middle of the splayed sliding groove 514 again, the medical staff sucks out the upper layer solution of the test tube 8, and the cells combined with the magnetic beads remain in the test tube 8; then the motor 521 works again, so that the elliptical sliding block 533 drives the clasp 532 to slide from the U-shaped limiting groove 513 on the left rotating disc 511 in the magnetic field separation chamber 12 into the U-shaped limiting groove 513 on the right rotating disc 512 in the placing chamber 11 again to complete the operation of the cells, and the medical staff can take out the test tube 8 containing the target cells from the sleeve 531;

[0058] In the utility model, the left rotating disc 511 and the right rotating disc 512 are driven to synchronously and reversely rotate through the driving gear 522 and the driven gear 523, the combination of the magnetic beads and the target cells is more complete, the cell solution finally separated is more close to the requirement, and the efficiency of the cell separation is improved; and the partition plate 4 divides the box body 1 into multiple chambers, not only improves the space utilization, but also separates the test tube 8 not subjected to the cell separation and the test tube 8 subjected to the cell separation in the box body 1, improves the practicality of the disinfection device, and is convenient to use.

[0059] In some embodiments, as shown in Figure 1 As a preferred embodiment of the utility model, the device has compact structure, small volume and is convenient for medical staff to carry.

[0060] The above embodiments are only used to illustrate the technical scheme of the utility model, rather than limit it; although the utility model is described in detail with reference to the foregoing embodiments, those skilled in the art should understand that the technical scheme recorded in the foregoing embodiments can be modified, or some technical features can be replaced equivalently; and the modification or replacement does not make the essence of the corresponding technical scheme deviate from the spirit and scope of the technical scheme of the embodiments of the utility model.

Claims

1. A cell sorting instrument, comprising a housing (1), characterized in that: The upper middle part of the box body (1) is hinged to the baffle (2); The pull rod (3) is fixedly installed on the upper end of the baffle (2); The bottom of the box (1) is fixedly connected to the bottom plate (7); A central control box (9) is fixedly installed on the outer end of the box body (1); The magnetic block (6) is fixedly installed on the inner left side of the box (1); Two partitions (4) are symmetrically fixedly installed on the front and rear sides of the inside of the box (1), dividing the inside of the box (1) into a placement chamber (11) and a magnetic field separation chamber (12), and a through groove is provided between the two partitions (4) to connect the two chambers to each other; the placement chamber (11) is used to store cell solution test tubes (8) that have been added with antibody-labeled magnetic beads, and the magnetic field separation chamber (12) is used to separate the cells in the test tubes (8) that have been adsorbed with magnetic beads and the cells that have not been adsorbed with magnetic beads; A circulating transfer mechanism (5) is installed on the base plate (7) to drive the test tubes (8) containing cell solution for cell sorting. The circulating transfer mechanism (5) includes a transfer component (51), a synchronous reverse drive component (52), and a carrier component (53). The lower end of the base plate (7) is equipped with a synchronous reverse drive component (52) for controlling the movement of the transfer component (51). The lower end of the inner side of the box (1) is equipped with a transfer component (51) for controlling the test tube (8) to change chambers. The transfer component (51) and the synchronous reverse drive component (52) are connected. The carrier component (53) is installed on the transfer component (51). The central control box (9) is electrically connected to the synchronous reverse drive component (52).

2. The cell sorting instrument according to claim 1, characterized in that, The magnetic block (6) is U-shaped.

3. The cell sorting instrument according to claim 2, characterized in that, The synchronous reverse drive assembly (52) includes a motor (521), a driving gear (522), a driven gear (523), and a transmission assembly. The motor (521) is fixedly mounted on the lower inner wall of the housing (1), and the motor (521) is connected to the transmission assembly. The drive gear (522) is rotatably connected to the lower left side of the base plate (7), and the driven gear (523) is rotatably connected to the lower right side of the base plate (7); the motor (521) and the drive gear (522) are connected to the transmission assembly; the motor (521) is electrically connected to the central control box (9).

4. The cell sorting instrument according to claim 3, characterized in that, The transmission assembly includes a worm gear (524) and a worm (525). The lower end of the drive gear (522) is fixedly connected to the worm gear (524), and the output end of the motor (521) is fixedly connected to one end of the worm (525). The worm gear (524) and the worm (525) are meshed together.

5. The cell sorting instrument according to claim 4, characterized in that, The material transfer assembly (51) includes a left turntable (511), a right turntable (512), a U-shaped limiting groove (513), and a figure-eight sliding groove (514). The left turntable (511) is rotatably mounted on the upper left side of the base plate (7) and is fixedly connected to the drive gear (522). The right turntable (512) is rotatably mounted on the upper right side of the base plate (7) and is fixedly connected to the driven gear (523). Multiple U-shaped limiting grooves (513) are provided on both the left turntable (511) and the right turntable (512). A figure-eight sliding groove (514) is provided on the upper end of the base plate (7).

6. The cell sorting instrument according to claim 5, characterized in that, The U-shaped limiting grooves (513) are evenly distributed at equal intervals around the left turntable (511) and the right turntable (512).

7. The cell sorting instrument according to claim 6, characterized in that, The bearing assembly (53) includes a sleeve (531), a retaining ring (532), and an elliptical sliding block (533). The retaining ring (532) is fixedly installed at the outer end of the middle part of the sleeve (531), and the elliptical sliding block (533) is fixedly installed at the bottom of the sleeve (531). The retaining ring (532) is slidably connected to the U-shaped limiting groove (513), and the elliptical sliding block (533) is slidably connected to the figure-eight sliding groove (514). The test tube (8) is inserted into the sleeve (531).

8. The cell sorting instrument according to claim 7, characterized in that, The inner diameter of the sleeve (531) is larger than the outer diameter of the test tube (8).

9. The cell sorting instrument according to claim 8, characterized in that, The middle part of the figure-eight groove (514) is the junction of the placement chamber (11) and the magnetic field separation chamber (12).

10. The cell sorting instrument according to claim 9, characterized in that, The baffle (2) is provided with a transparent observation window.

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