Detection analyzer based on kit

Abstract

The invention provides a detection analyzer based on a kit, and the detection analyzer comprises a housing, the outer wall of which is provided with a warehouse outlet facing a first direction; the machine core module is arranged in the machine shell, and the machine core module is used for accommodating the kit and pre-treating and detecting an object to be detected in the kit; the delivery module is arranged in the machine shell and is connected with the machine core module; the delivery module can reciprocate along the first direction relative to the delivery port so as to drive the machine core module to go in and out of the delivery port. The integration level and the detection efficiency of a detection instrument based on the kit are improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biological detection, and particularly relates to a detection analyzer based on a kit. BACKGROUND

[0002] Detection analyzers are commonly used in the fields of biological and chemical experimental research. For example, a nucleic acid detection analyzer is used to detect nucleic acids in biological detection. Before detecting nucleic acids, cells in a sample to be detected are generally subjected to a pretreatment step such as lysis to obtain relatively pure nucleic acid templates, and then nucleic acid amplification is performed by means such as polymerase chain reaction (PCR), recombinase polymerase isothermal amplification (RPA), loop-mediated isothermal amplification (LAMP), and the like, and the amplified nucleic acids are then detected.

[0003] To reduce contamination caused by contact between reagents and the outside world during detection, the applicant designed an integrated kit, which has cavities corresponding to lysis, pipetting, and amplification. Although the integrated kit can isolate the reagents from the outside world, the operation of the integrated kit requires multiple instruments to complete. That is, lysis, pipetting, quantification, and amplification of the sample in the kit all require different instruments to achieve. This not only increases the workload of the operator and reduces the detection efficiency. When moving the integrated kit, it may also cause the kit to fall over, fall, and the like, resulting in a decrease in the accuracy of the experimental results. It can be seen that the degree of integration and automation of the instruments for processing the integrated kit in the prior art is very low, which seriously affects the detection efficiency and detection accuracy. SUMMARY

[0004] The present application aims to solve the technical problems of low integration and low detection efficiency of detection instruments based on kits. The present application provides a detection analyzer based on a kit, which can improve the integration and detection efficiency of detection instruments based on kits.

[0005] To solve the above technical problems, the embodiments of the present application provide a detection analyzer based on a kit, which comprises: a housing, an ejection port facing a first direction is arranged on an outer wall of the housing; a core module arranged in the housing, the core module being used for accommodating the kit and performing pretreatment and detection on the sample in the kit; an ejection module arranged in the housing and connected with the core module; the ejection module is capable of reciprocating relative to the ejection port along the first direction to drive the core module to enter or exit the ejection port.

[0006] In the optional embodiments of the present application, a bottom plate is arranged at the bottom of the housing, the bottom plate is detachably connected with the housing, and the ejection module comprises: The first guide seat is arranged on the bottom plate, and a first guide groove extending in the first direction is arranged on the first guide seat. The first guide rail is arranged at the bottom of the core module and extends in the first direction, is inserted into the first guide groove, and the outer wall of the first guide rail is matched with the inner wall of the first guide groove. The first guide rail can reciprocate along the first direction relative to the first guide seat. The first driving module is arranged on the bottom plate, and the driving end of the first driving module is connected with the core module. The first driving module is used for driving the core module to reciprocate in the first direction.

[0007] In an optional case of the present application, the kit is internally provided with a lysis section and an amplification tube arranged in a vertical direction. The lysis section is used for containing the test object and pre-treating the test object. The amplification tube is used for amplifying the pre-treated test object under a preset condition.

[0008] In an optional case of the present application, the core module comprises: The mounting plate is arranged at the bottom of the core module. The lysis module is arranged above the mounting plate. The lysis module is internally provided with a lysis cavity. The first temperature controller is arranged in the lysis cavity. The amplification module is arranged on the mounting plate and connected with the bottom of the lysis module. The amplification module is internally provided with an amplification cavity. The amplification cavity is in communication with the lysis cavity. The second temperature controller is arranged in the amplification cavity. When the kit is accommodated in the core module, the lysis section is located in the lysis cavity, and the amplification tube is located in the amplification cavity. The first temperature controller is in contact with the outer wall of the lysis section, and the second temperature controller is in contact with the outer wall of the amplification tube.

[0009] In an optional case of the present application, the kit further comprises a quantitative tube arranged in parallel with the lysis section. When the kit is accommodated in the core module, the quantitative tube is located in the lysis cavity. The third temperature controller is arranged in the lysis cavity and in contact with the outer wall of the quantitative tube. The third temperature controller is used for controlling the temperature in the quantitative tube.

[0010] In an optional case of the present application, the kit comprises a plurality of input channels and at least one output channel, and a regulating valve arranged between the input channels and the output channel. The lysis section is part of one of the input channels, the quantitative tube is part of another input channel, and the amplification tube is in communication with the output end of the output channel. The regulating valve is internally provided with a plurality of conveying channels and can reciprocate along the length direction of the regulating valve. When the regulating valve is moved to a specific position, the corresponding conveying channel can connect the two input channels or can connect one of the input channels with one of the output channels.

[0011] In an optional solution of the present application, the movement module further includes a regulating module connected to the regulating valve, the regulating module is used to drive the regulating valve to move to a specific position along the length direction, and the regulating module includes: The mounting bracket is provided on the movement module; The movable bracket is connected to the mounting bracket and can move back and forth relative to the mounting bracket along the length direction; the movable bracket is provided with two pins, and the end faces of each pin are respectively in contact with the two end faces of the regulating valve; The second driving module is arranged on the mounting bracket. The driving end of the second driving module is connected to the movable bracket. The second driving module is used to drive the movable bracket to move back and forth relative to the mounting bracket along the length direction.

[0012] In an optional solution of the present application, a pressurizing piston is provided in at least one input channel, and the pressurizing piston is used to drive the pressurizing piston to move toward the delivery channel to drive the liquid in the input channel to flow into the delivery channel.

[0013] In an optional solution of the present application, a base plate is provided at the bottom of the casing, a frame is provided on the base plate, and at least one piston drive module is provided on the frame. The piston drive module is used to drive the pressurizing piston in the corresponding input channel to move toward the delivery channel.

[0014] In an optional solution of the present application, the piston drive module includes: A third driving module is provided on the frame and is located above the core module, with a driving end of the third driving module facing downward and capable of reciprocating in a vertical direction; A piston pressure column is connected to the driving end of the third driving module; the projection of the piston pressure column in the vertical direction falls on the upper end surface of the pressurizing piston; A limiting pressure block is provided at the bottom end of the piston pressure column, and the projection of the limiting pressure block in the vertical direction falls on the top surface of the main shell of the reagent box; The pressure block spring is arranged between the limiting pressure block and the piston pressure column. The pressure block spring and the limiting pressure block are used to apply downward pressure to the main shell of the reagent box, so that the reagent box is pressed against and fits the contact surface inside the movement module.

[0015] Compared with the prior art, the present invention has the following beneficial effects: The embodiment of the present invention is to set a discharge module inside the detection analyzer and connect the core module to the discharge module, so that the core module can be pushed out of the casing. It is convenient for the operator to place the test kit inside the core module or remove the test kit from the inside of the core module. Compared with the operation method of the prior art that requires manual opening of the analyzer protective cover, the present application can reduce the manual operation of the operator and improve the automation level of the detection analyzer. It prevents the operator from contacting objects outside the test kit, effectively reducing the risk of the test kit being contaminated. It improves the integration of the detection analyzer and improves the detection efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] To more clearly illustrate the specific embodiments of this application or the technical solutions in the prior art, the following briefly introduces the drawings required for the specific embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of this application. For those skilled in the art, other drawings can be derived from these drawings without inventive effort.

[0017] Figure 1 The figure shows the overall appearance of the detection analyzer provided by one embodiment of the present invention; Figure 2 FIG2 shows an internal structure view of a detection analyzer provided by an embodiment of the present invention; Figure 3 The figure shows a structural view of the ejection module and the movement module provided in one embodiment of the present invention, so as to demonstrate the connection relationship between the ejection module and the movement module; Figure 4 A structural view of a regulating module and a lysis module provided in one embodiment of the present invention is shown to illustrate the connection relationship between the regulating module and the lysis module; Figure 5 A cross-sectional view of a reagent kit and a lysis module provided in one embodiment of the present invention is shown, wherein the amplification module is hidden to show the internal structures of the lysis module and the amplification module; Figure 6 The figure shows a reagent kit, an amplification module, and an optical detection assembly provided in one embodiment of the present invention, so as to demonstrate the connection relationship between the amplification module, the reagent kit, and the optical detection assembly; Figure 7 1. It shows a structural view of a piston drive module provided by one embodiment of the present invention; Figure 8 The figure shows the overall structure of the kit provided by one embodiment of the present invention; Figure 9 A cross-sectional view of a kit provided by one embodiment of the present invention is shown to illustrate the lysis channel, the injection channel, and the quantitative channel; Figure 10 Shown Figure 9 A partial enlarged view of part A in the middle, showing the internal communication passage a of the reagent box when the regulating valve is in the first position; Figure 11 A cross-sectional view of a kit provided by another embodiment of the present invention is shown to illustrate the quantitative channel, the pipetting channel, and the first channel; Figure 12 Shown Figure 11 A partial enlarged view of portion B in the middle section, showing the internal communication passage b of the reagent box when the regulating valve is in the second position; Figure 13A sectional view of the kit provided by another embodiment of the present application is shown to show the storage channel, the replenishment channel and the second channel; Figure 14 A sectional view of the kit provided by another embodiment of the present application is shown to show the storage channel, the replenishment channel and the second channel; Figure 13 A sectional view of the kit provided by another embodiment of the present application is shown to show the storage channel, the replenishment channel and the second channel.

[0018] Wherein, the reference signs are as follows: 1. main housing, 2. connecting column, 3. amplification tube, 4. storage member, 5. storage space, 6. adjusting valve, 7. conveying channel, 8. pipetting channel, 9. liquid injection channel, 10. replenishment channel, 11. adjusting channel, 12. input channel, 13. lysis channel, 14. pressurizing section, 15. lysis section, 16. feed piston, 17. feed hole, 18. piston cover, 19. metering channel, 20. metering tube, 21. waste tube, 22. sealing cover, 23. storage channel, 24. replenishment piston, 25. piston press block, 26. pressure zone, 27. storage tube, 28. output channel, 29. first channel, 30. second channel, 31. extension tube, 32. machine housing, 33. ejection port, 34. core module, 35. kit, 36. ejection module, 37. bottom plate, 38. first guide seat, 39. first guide slot, 40. first guide rail, 41. first drive module, 42. motor, 43. actuator, 44. synchronous belt, 45. synchronous pulley, 46. mounting plate, 47. lysis module, 48. lysis cavity, 50. amplification module, 51. amplification cavity, 52. temperature bath block, 53. adjusting module, 54. mounting support, 55. moving support, 56. pin column, 57. second drive module, 58. pressurizing piston, 59. machine frame, 60. piston drive module, 61. third drive module, 62. piston press column, 63. limit press block, 64. press block spring, 65. containing cavity, 66. clearance hole, 68. optical detection assembly, 69. excitation light assembly, 70. excitation light channel, 73. electromagnet, 77. Peltier, 78. finned radiator, 79. cooling fan, 80. door, 81. guide rail support, 82. mounting back plate, 83. second guide seat, 84. second guide slot, 85. second guide rail, 86. heating aluminum block, 87. heating rod, 88. heat conducting block. DETAILED DESCRIPTION

[0019] The following detailed description together with the accompanying drawings will provide a fuller understanding of the application. Although the application is described with reference to the preferred embodiment, those skilled in the art will readily appreciate that the detailed description and drawings are by way of illustration only. Merely by way of example, the description of the application is not intended to limit the scope of the application to particular embodiments described herein. The description of the application together with the drawings will provide those of ordinary skill in the art with a complete understanding of the application. The application is not limited to the particular embodiments described herein. The drawings are not necessarily to scale, emphasis instead being placed upon illustrating the principles of the application. The detailed description is presented only for the purpose of providing a thorough understanding of the application. Those skilled in the art will recognize that the description and specific examples are not intended to limit the application to the specific forms disclosed. Rather, they are included to disclose some embodiments of the application. One skilled in the art will readily recognize from the disclosure herein, that other alternatives to the examples and preferred embodiments described herein can be employed and that many modifications, variations and changes in detail can be

[0020] It should be noted that in this specification and the accompanying drawings, similar reference numerals and letters in different drawings represent similar elements, so once an element is defined in one drawing, it need not be further defined and explained in subsequent drawings.

[0021] In the description of the present embodiments, it should be noted that the terms "upper", "lower", "inner", "bottom", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship in which the product of the application is usually placed, which is only for the convenience of describing the application and simplifying the description, and does not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the application.

[0022] The terms "first", "second", etc. are only used for differentiation in description and cannot be understood as indicating or implying relative importance.

[0023] In the description of the present embodiments, it should also be noted that, unless otherwise explicitly specified and limited, the terms "provided", "connected", "connected" should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium, or it can be connected inside two elements. For those skilled in the art, the specific meaning of the above terms in the present embodiments can be understood according to the specific circumstances.

[0024] In order to make the objects, technical solutions and advantages of the present application clearer, the embodiments of the present application will be further described in detail below with reference to the drawings.

[0025] In order to make the objects, technical solutions and advantages of the present application clearer, the embodiments of the present application will be further described in detail below with reference to the drawings. Please refer to Figures 1 to 14 , Figure 1The figure shows the overall appearance of a detection analyzer provided by one embodiment of the present invention. Figure 2 The figure shows the internal structure of the detection analyzer provided by one embodiment of the present invention. Figure 3 The figure shows a structural view of a bin ejection module and a movement module provided by an embodiment of the present invention, so as to demonstrate the connection relationship between the bin ejection module and the movement module. Figure 4 A structural view of an adjustment module and a lysis module provided in one embodiment of the present invention is shown to illustrate the connection relationship between the adjustment module and the lysis module. Figure 5 A cross-sectional view of a reagent kit and a lysis module according to an embodiment of the present invention is shown, wherein the amplification module is hidden to show the internal structures of the lysis module and the amplification module. Figure 6 The reagent kit, amplification module and optical detection assembly provided by one embodiment of the present invention are shown to demonstrate the connection relationship between the amplification module, the reagent kit and the optical detection assembly. Figure 7 A structural view of a piston drive module provided by an embodiment of the present invention is shown. Figure 8 The figure shows the overall structure of the kit provided by one embodiment of the present invention. Figure 9 A cross-sectional view of a kit provided in accordance with an embodiment of the present invention is shown to illustrate a lysis channel, an injection channel, and a quantitative channel. Figure 10 Shown Figure 9 A partial enlarged view of part A in the figure shows the internal communication passage a of the reagent box when the regulating valve is in the first position. Figure 11 A cross-sectional view of a reagent kit provided by another embodiment of the present invention is shown to illustrate the quantitative channel, the pipetting channel and the first channel. Figure 12 Shown Figure 11 A partial enlarged view of part B in the figure shows the internal connecting passage b of the reagent box when the regulating valve is in the second position. Figure 13 A cross-sectional view of a reagent kit provided in accordance with yet another embodiment of the present invention is shown to illustrate the liquid storage channel, the liquid replenishment channel, and the second channel. Figure 14 Shown Figure 13 A partial enlarged view of part C in the middle shows the internal connecting passage c of the reagent kit when the regulating valve is in the third position.

[0026] The embodiment of the present invention provides a detection analyzer based on a kit, such as Figure 1 and Figure 2 As shown, the detection analyzer includes a housing 32, a core module 34 and a bin module 36. The outer wall of the housing 32 is provided with a first direction ( Figures 1 to 6The machine core module 34 is arranged inside the machine shell 32, and is used to accommodate the reagent box 35 and pre-treat and detect the to-be-tested substance in the reagent box 35. The ejection module 36 is arranged inside the machine shell 32 and connected with the machine core module 34; the ejection module 36 is capable of reciprocating relative to the ejection opening 33 along the first direction N, so as to drive the machine core module 34 to enter or exit the ejection opening 33.

[0027] By arranging the ejection module 36 inside the detection analyzer and connecting the machine core module 34 with the ejection module 36, the machine core module 34 can be pushed out of the machine shell 32. This facilitates the operator to place the reagent box 35 into the machine core module 34 or remove the reagent box 35 from the machine core module 34. Compared with the operation mode of manually opening the protective cover of the analyzer in the prior art, the present application can reduce the manual operation of the operator and improve the automation level of the detection analyzer. The operator is prevented from contacting the items outside the reagent box 35, effectively reducing the risk of contamination of the reagent box 35. Compared with the cumbersome operation of multiple instruments in the prior art, the detection analyzer provided by the present application realizes the experimental operation of the reagent box 35 through each module, improves the instrument integration and detection efficiency, reduces the detection error caused by switching instruments, and improves the detection accuracy.

[0028] Further, as shown in FIG. 1, the detection analyzer 1 comprises a machine shell 32, an ejection opening 33, a machine core module 34, and an ejection module 36. Figure 2 and Figure 3As shown, the bottom of the casing 32 is provided with a bottom plate 37, and the bottom plate 37 is detachably connected with the casing 32. The warehouse module 36 comprises a first guide seat 38, a first guide rail 40 and a first driving module 41. The first guide seat 38 is arranged on the bottom plate 37, and the first guide seat 38 is provided with a first guide groove 39 extending along the first direction N. The first guide rail 40 is arranged at the bottom of the core module 34 and extends along the first direction N. The first guide rail 40 is inserted into the first guide groove 39, and the outer wall of the first guide rail 40 is matched with the inner wall of the first guide groove 39. The first guide rail 40 can reciprocate along the first direction N relative to the first guide seat 38. The first driving module 41 is arranged on the bottom plate 37, and the driving end of the first driving module 41 is connected with the core module 34. The first driving module 41 is used to drive the core module 34 to reciprocate along the first direction N. Specifically, the number of the first guide seat 38 and the first guide rail 40 is both two. The bottom of the core module 34 is provided with two U-shaped guide rail supports 81, and the first guide rail 40 is connected with the guide rail support 81. The guide rail support 81 comprises two vertical parts and a horizontal part extending along the first direction N. The upper end of each vertical part is connected with the lower surface of the mounting plate 46. The upper surface of the first guide rail 40 is connected with the lower surface of the horizontal part and is matched with the lower surface. The space between the two first guide rails 40 is formed to facilitate the arrangement of the heat dissipation module below the mounting plate 46. The first guide rail 40 and the first guide groove 39 can also be applied with lubricating grease to reduce the friction between the first guide rail 40 and the first guide groove 39.

[0029] Further, as Figure 3As shown, the first driving module 41 comprises a motor 42 and an actuator 43 connected with the core module 34, and the motor 42 is configured to drive the actuator 43 to drive the core module 34 to reciprocate relative to the motor 42 along the first direction N. The structure of the actuator 43 is not limited herein, and any device capable of driving the core module 34 to reciprocate along the first direction N in and out of the outlet 33 is within the protection scope of the present application. For example, the actuator 43 can comprise a synchronous belt 44 and a synchronous belt 44 wheel, or a screw-nut mechanism, or a gear and rack mechanism. Specifically, when the actuator 43 comprises the synchronous belt 44 and two synchronous belt 44 wheels, each synchronous belt 44 wheel is arranged on the bottom plate 37, and one of the synchronous belt 44 wheels is connected with the driving end of the motor 42; the synchronous belt 44 is sleeved on the outer periphery of each synchronous belt 44 wheel, and the length direction of the synchronous belt 44 extends along the first direction N; the core module 34 is connected with a point on the synchronous belt 44, and when the motor 42 drives the synchronous belt 44 to reciprocate, the core module 34 can reciprocate along the first direction N with the synchronous belt 44. When the actuator 43 comprises a screw and a nut, the screw is connected with the driving end of the motor 42 and extends along the first direction N, the nut is arranged on the core module 34, and the screw and the nut are threadedly connected; when the motor 42 drives the screw to reciprocate, the core module 34 reciprocates along the first direction N with the nut. When the actuator 43 comprises a gear and a rack, the gear is connected with the driving end of the motor 42, the rack is arranged on the core module 34 and extends along the first direction N, the rack is engaged with the gear, and when the motor 42 drives the gear to reciprocate, the core module 34 reciprocates along the first direction N with the rack.

[0030] Further, as shown in Figure 5 、 Figure 9 、 Figure 11 and Figure 13 , the kit 35 is internally provided with the lysis section 15 and the amplification tube 3 which are arranged in a vertical direction, the lysis section 15 is configured to accommodate the to-be-tested substance and pre-process the to-be-tested substance, and the amplification tube 3 is configured to amplify the pre-processed to-be-tested substance under a preset condition.

[0031] Further, as shown in Figure 3As shown, the core module 34 includes a mounting plate 46, a lysis module 47, and an amplification module 50. The mounting plate 46 is arranged at the bottom of the core module 34. The lysis module 47 is arranged above the mounting plate 46, and the lysis module 47 is internally provided with a lysis cavity 48, and the lysis cavity 48 is internally provided with a first temperature controller. The amplification module 50 is arranged on the mounting plate 46 and connected to the bottom of the lysis module 47; the amplification module 50 is internally provided with an amplification cavity 51, the amplification cavity 51 is in communication with the lysis cavity 48, and the amplification cavity 51 is internally provided with a second temperature controller. When the kit 35 is received in the core module 34, the lysis section 15 is located in the lysis cavity 48, and the first temperature controller is in contact with the outer wall of the lysis section 15; the amplification tube 3 is located in the amplification cavity 51, and the second temperature controller is in contact with the outer wall of the amplification tube 3. Specifically, the first temperature controller and the second temperature controller are both aluminum blocks, the first temperature controller is provided with a groove matched with the lysis section 15, and the inner wall of the groove is in contact with the outer wall of the lysis section 15. The second temperature controller is provided with a receiving cavity 65 matched with the outer wall of the amplification tube 3, and the inner wall of the receiving cavity 65 is in contact with the outer wall of the amplification tube 3.

[0032] Further, as shown in Figure 2 and Figure 6 , the first temperature controller includes a heating aluminum block 86, a heating rod 87, and a first sensor. The heating aluminum block is provided with a first heating surface, which is in contact with the outer wall of the lysis section 15 to transfer heat to the lysis section 15. The heating rod 87 is in communication with the control system of the detection analyzer, and the heating section of the heating rod 87 is connected to the heating aluminum block 86. The heating rod 87 is used to heat the heating aluminum block 86 according to the command of the control system of the detection analyzer; the heating aluminum block 86 acts as a heat reservoir for the heating rod 87 to store heat and transfer heat to the lysis section 15 through the first heating surface, so as to continuously transfer heat to the lysis section 15 when the heating rod 87 stops heating. The first sensor is connected to the heating aluminum block 86, and the first sensor is a temperature sensor for measuring the temperature of the heating aluminum block 86 and feeding back to the control system of the detection analyzer. The control system can control the heating rod 87 to heat when the temperature of the heating aluminum block 86 is lower than the lower limit of the preset temperature range, and control the heating rod 87 to stop heating when the temperature of the heating aluminum block 86 is higher than the upper limit of the preset temperature range. In addition, the lysis module 47 is provided with a gap on the side wall close to the lysis section 15, and the heating aluminum block 86 is inserted into the gap and extends into the inside of the lysis cavity 48.

[0033] Further, as shown in Figure 5 and 6As shown, the second temperature controller comprises a thermal bath block 52, a Peltier 77 and a second sensor. The top surface of the thermal bath block 52 is provided with a receiving cavity 65, and the outer wall of the amplification tube 3 is fitted with the inner wall of the receiving cavity 65 to exchange heat with the substance in the amplification tube 3. The Peltier 77 is arranged below the thermal bath block, and the first surface of the Peltier 77 is fitted with the bottom surface of the thermal bath block 52. The Peltier 77 is used to heat or cool the thermal bath block. The second sensor is connected with the Peltier 77, and the second sensor is a temperature sensor used to measure the temperature of the thermal bath block and feed back to the control system of the detection analyzer. The control system can cool when the temperature of the thermal bath block 52 is higher than the upper limit of the preset temperature range, and heat when the temperature of the thermal bath block 52 is lower than the lower limit of the preset temperature range, so that the temperature of the thermal bath block 52 is kept within the preset temperature range. In other embodiments, the control system can also control the temperature of the thermal bath block 52 to be constant or variable according to the preset program, so that the nucleic acid in the amplification tube 3 is subjected to constant temperature amplification or programmed temperature amplification.

[0034] Further, as shown in Figure 5 and Figure 9 The kit 35 also comprises a quantification tube 20 arranged in parallel with the lysis section 15. When the kit 35 is received in the core module 34, the quantification tube 20 is located in the lysis cavity 48, and the lysis cavity 48 is provided with a third temperature controller which is fitted with the outer wall of the quantification tube 20. Specifically, the third temperature controller comprises a heat conduction block 88, a Peltier 77 and a third sensor. The heat conduction block 88 is provided with a second heating surface which is fitted with the outer wall of the quantification tube 20 to exchange heat with the substance inside the quantification tube 20. The Peltier 77 is arranged on the lysis module 47, and the first surface of the Peltier 77 is fitted with the heat conduction block. The Peltier 77 is used to heat or cool the heat conduction block 88. The third sensor is connected with the Peltier 77, and the third sensor is used to measure the temperature of the heat conduction block 88 and feed back to the control system of the detection analyzer. The control system is used to control the Peltier 77 to heat when the temperature of the heat conduction block 88 is lower than the lower limit of the preset temperature range, and control the Peltier 77 to cool when the temperature of the heat conduction block 88 is higher than the upper limit of the preset temperature range, so that the temperature of the heat conduction block 88 is kept within the preset temperature range. Specifically, since the sample has been heated by the first temperature controller in the lysis section 15, but after the sample is transported into the quantification tube 20, the sample needs to be first cooled by the Peltier 77 to stabilize the temperature of the sample within the preset temperature range. Generally, the sample can be output into the amplification tube 3 when the temperature of the sample is stabilized within the preset range, and heating is not needed at this time.

[0035] Furthermore, for the second and third thermostats, a finned heat sink 78 is provided on one side of the second surface of the Peltier 77. The mounting end surface of the finned heat sink 78 is in contact with the second surface of the Peltier 77. A cooling fan 79 is provided on the side of the finned heat sink 78 facing away from the Peltier 77. The cooling fan 79 is used to drive external air through the finned heat sink 78 to exchange heat with the finned heat sink 78. Specifically, the cooling fan 79 blows air toward the interior of the finned heat sink 78. After entering the finned heat sink 78, the air exchanges heat with the fins. When the Peltier 77 is heating the finned heat sink 78, heat is transferred from the fins to the air, cooling the fins. When the Peltier 77 is cooling the finned heat sink 78, heat is transferred from the air to the fins, heating the fins. Specifically, a clearance opening is provided on the mounting plate 46, and the Peltier 77 of the second temperature controller is disposed within the clearance opening of the mounting plate 46, with the corresponding finned heat sink 78 disposed below the mounting plate 46. A clearance opening is also provided on the mounting back plate 82, and the Peltier 77 of the second temperature controller is disposed within the clearance opening of the mounting back plate 82, with the corresponding finned heat sink disposed on the side of the mounting back plate 82 away from the cracking module 47.

[0036] Further, if Figure 5 、 Figure 9 、 Figure 11 and Figure 13 As shown, the reagent kit 35 includes multiple input channels 12 and at least one output channel 28, and a regulating valve 6 provided between the input channels 12 and the output channels 28; the lysis section 15 is a part of one of the input channels 12, and the amplification tube 3 is connected to the output end of the output channel 28; the regulating valve 6 is provided with multiple delivery channels 7, and the regulating valve 6 can be adjusted along its own length direction ( Figures 1 to 6 The regulating valve 6 is configured to reciprocate in the direction M (as shown in the center); when the regulating valve 6 moves to a specific position, the corresponding delivery channel 7 can connect two of the input channels 12, or can connect one of the input channels 12 to one of the output channels 28. The specific number of input channels 12 and output channels 28 of the reagent kit 35 is not limited here. Any reagent kit 35 having a lysis section 15 and an amplification tube 3 and compatible with the lysis module 47 and amplification module 50 of this application is within the scope of protection of this application. To facilitate understanding by those skilled in the art, the following examples are listed to introduce the reagent kit 35 involved in this application.

[0037] Example 1 like Figure 8As shown, the kit 35 comprises a main housing 1 and a regulating valve 6. The main housing 1 is internally configured with a regulating channel 11 extending in the horizontal direction, a plurality of input channels 12 are arranged above the regulating channel 11, and a plurality of output channels 28 are arranged below the regulating channel 11. The regulating valve 6 is arranged in the regulating channel 11 and is movable in the regulating channel 11; the regulating valve 6 is provided with a plurality of delivery channels 7; when the regulating valve 6 is moved to a specific position, the corresponding delivery channel 7 can connect two of the input channels 12 or can connect one of the input channels 12 with one of the output channels 28. By arranging the regulating channel 11 in the main housing 1, arranging the input channels 12 above the regulating channel 11, and arranging the output channels 28 below the regulating channel 11, and moving the regulating valve 6 in the regulating channel 11 can achieve the connection between two of the input channels 12 or the connection between one of the input channels 12 and one of the output channels 28. Thus, the liquid transfer, reagent mixing, and reagent output can be achieved in a fully closed environment. Compared with the prior art, the kit 35 provided by the present application integrates a plurality of cavities in the same main housing 1, and the cavities can be selectively connected, thereby improving the integration level of the kit 35. The experimental operation steps are reduced, the biological detection efficiency is improved, the contact time between the reagents and the outside world is reduced, and the reagent pollution caused by reagent transfer is avoided.

[0038] It is worth mentioning that the kit 35 provided by the present application can be used for nucleic acid detection, but does not exclude the use of the kit 35 in other biological detection, biological experiments, and chemical experiments. Any kit 35 having the same or substantially same structure as the kit 35 provided by the present application, whether used for one or more of reagent quantification, reagent mixing, reagent transfer, and reagent culture, is within the protection scope of the present application. In some large-scale biological detection or chemical detection, a plurality of reaction reagents or culture reagents are included, and different reagents can be respectively stored in a plurality of input channels 12, or different reagents can be respectively quantified and processed. The number of the input channels 12 and the output channels 28 is not limited herein, and a person skilled in the art can set the number of the input channels 12 and the output channels 28 according to the number of reagents and the processing steps in biological detection or chemical detection.

[0039] Further, as shown in Figure 11 and Figure 13 , the input channel 12 comprises a lysis channel 13, a quantification channel 19, and a storage channel 23 arranged in the horizontal direction (specifically, the width direction of the kit 35, as shown in the Y direction in Figures 8 to 13 , the lysis channel 13 is arranged above the quantification channel 19, and the storage channel 23 is arranged below the quantification channel 19. Figure 13As shown in FIG. 1, the main housing 1 comprises a plurality of channels, including a sample input channel 12, a sample transfer channel 7, a sample lysis channel 13, a sample quantification channel 19, a sample storage channel 23, and a sample output channel 28. In some embodiments, the sample input channel 12 is configured to receive a sample to be tested, and the sample transfer channel 7 is configured to transfer the sample to be tested to the sample lysis channel 13. In some embodiments, the sample lysis channel 13 is configured to receive and lyse the sample to be tested, and the sample quantification channel 19 is configured to determine a preset volume of the sample to be tested and transfer the sample to the sample output channel 28. In some embodiments, the sample storage channel 23 is configured to store and deliver a liquid reagent to the sample output channel 28. Specifically, the liquid reagent can be the same as the solvent of the sample to be tested, or can be a reagent that generates a biochemical reaction with the sample to be tested, or can be a buffer or pure water. The operator can add the corresponding liquid reagent into the sample storage channel 23 according to the experimental requirements. It is particularly pointed out that the number of sample storage channels 23 can be multiple, and the multiple sample storage channels 23 can respectively store different types and functions of liquid reagents, and the multiple liquid reagents are used for different steps in the same biochemical experiment. In other embodiments, the sample lysis channel 13 can be used for sample storage, heat treatment, biological culture and other processing steps, and the sample storage channel 23 can also be used for storing reagents and / or processing reagents (such as heating, light irradiation, etc.). Specifically, the sample to be tested is a flowable fluid sample, which can be a sample aqueous solution and suspension, or a substance that can be mixed with other reagents into a fluid in the kit 35 provided by the present application, that is, the sample to be tested needs to be able to flow inside the sample input channel 12, the sample transfer channel 7 and the sample output channel 28.

[0040] Further, as shown in FIG. 1, Figure 9 and Figure 11 the sample lysis channel 13 comprises a pressurization section 14 and a lysis section 15 arranged in sequence from top to bottom, and the pressurization section 14 is provided with a feeding piston 16. Specifically, the cross section (i.e. the cross section in the horizontal direction, the same below) of the pressurization section 14 and the feeding piston 16 is circular, and the cross-sectional area of the pressurization section 14 is smaller than that of the lysis section 15, and a step is formed between the pressurization section 14 and the lysis section 15, which can limit the lowest height of the feeding piston 16 moving downward. The bottom surface of the lysis section 15 is provided with an output port for communicating with the sample transfer channel 7, and the feeding piston 16 is used to increase the air pressure in the sample lysis channel 13 to inject the sample to be tested into the sample transfer channel 7. In some embodiments, the sample to be tested is a biological cell or a biological cell metabolite containing a target nucleic acid sequence, and the side wall of the lysis section 15 is configured to transfer heat to the sample lysis channel 13 to heat the sample to be tested, so that the sample to be tested is lysed to release the target nucleic acid sequence. Preferably, in order to facilitate heat transfer, the main housing 1 can be integrally processed by using a material that is easy to conduct heat. In order to save materials, the main housing 1 can be processed in different regions, and the side wall of the lysis section 15 can be processed by using a material that is easy to conduct heat. In other embodiments, the sample to be tested can be a solution or suspension of biological material or synthetic material, or a solution or suspension of ore or alloy powder, and the lysis section 15 can be used for lysing, dissolving, decomposing and other pretreatment of the sample to be tested, to prepare for the next step of the experiment.

[0041] Further, as shown in FIG. 1, Figures 8 to 13As shown, the feed piston 16 is constructed with a Figures 8 to 13 The feed hole 17 is connected to the reagent box 35 (as shown in the X direction in the middle), and the feed hole 17 is used to add the analyte to the cleavage channel 13; the upper end of the feed hole 17 is provided with a piston cover 18 for sealing the feed hole 17. Specifically, when the reagent box 35 is not activated, the piston cover 18 tightly covers the feed hole 17 to ensure that the internal space of the reagent box 35 is isolated from the outside world and prevent the interior of the reagent box 35 from being contaminated. When the analyte needs to be added to the reagent box 35, the piston cover 18 is opened and the analyte is quickly injected into the feed hole 17. The piston cover 18 is then restored to the state of sealing the feed hole 17. On the one hand, it can prevent the interior of the reagent box 35 from being contaminated. On the other hand, when the piston pushes the analyte downward to be injected into the delivery channel 7, the piston cover 18 can prevent the cleavage channel 13 from depressurizing, ensuring that the air pressure in the cleavage channel 13 increases to output the analyte downward.

[0042] Furthermore, if Figure 13 As shown, the delivery channel 7 includes a pipetting channel 8, an injection channel 9 and a rehydration channel 10 arranged at intervals. When the regulating valve 6 moves to the first position, one end of the pipetting channel 8 is connected to the lysis channel 13, and the other end is connected to the quantitative channel 19. Figure 10 As shown in the middle passage a. When the regulating valve 6 moves to the first position, the feed piston 16 moves downward to increase the air pressure inside the cleavage channel 13, so that the analyte inside the cleavage channel 13 is injected into the pipetting channel 8 from the output port at the lower end of the cleavage channel 13, and enters the quantitative channel 19 along the pipetting channel 8 to measure the preset volume of the analyte. When the regulating valve 6 moves to the second position, one end of the injection channel 9 is connected to the quantitative tube 20, and the other end is connected to the output channel 28, as shown in the middle passage a. Figure 12 As shown in the middle passage b. Since the air pressure in the quantitative channel 19 is increased when the feed piston 16 injects the analyte into the quantitative channel 19, when the quantitative tube 20 is connected to the output channel 28 through the injection channel 9, the air pressure in the quantitative channel 19 is greater than the air pressure in the output channel 28. Therefore, the analyte in the quantitative channel 19 can automatically flow into the output channel 28 under the action of the air pressure difference, realizing the reagent transfer of the preset volume of the analyte. When the regulating valve 6 moves to the third position, one end of the rehydration channel 10 is connected to the liquid storage channel 23, and the other end is connected to the output channel 28, as shown in the third position. Figure 14 As shown in the middle passage c, it is convenient to mix the liquid reagent with the aforementioned preset volume of the analyte.

[0043] Furthermore, if Figure 9 and Figure 11As shown, the quantification channel 19 comprises a quantification tube 20 and a waste tube 21 arranged in parallel along the horizontal direction; the volume of the quantification tube 20 is equal to the preset volume of the sample to be measured, and the upper end of the quantification tube 20 is in communication with the waste tube 21. When the adjusting valve 6 is moved to the first position, the lower end of the quantification tube 20 is in communication with the pipetting channel 8. The sample to be measured output by the pipetting channel 8 first enters the quantification tube 20, and after the quantification tube 20 is filled with liquid, the sample to be measured overflows from the top end of the quantification tube 20 into the waste tube 21. When the quantification tube 20 is filled with liquid, the preset volume of the sample to be measured is obtained, and at the same time, the excess sample to be measured liquid overflows into the waste tube 21 and is separated from the sample to be measured liquid in the quantification tube 20. When the liquid in the quantification tube 20 flows out from the lower end of the quantification tube 20, the gas in the quantification channel 19 can enter the quantification tube 20, and the excess sample to be measured is retained in the waste tube 21, which can ensure that the volume of the sample to be measured in the quantification tube 20 remains unchanged. When the adjusting valve 6 is moved to the second position, the lower end of the quantification tube 20 is in communication with the injection channel 9, so that the preset volume of the sample to be measured obtained by the quantification tube 20 can flow into the output channel 28, realizing the reagent transfer of the preset volume of the sample to be measured. Preferably, the quantification tube 20 is arranged near the side wall of the quantification channel 19, and the side wall of the quantification channel 19 is configured to be able to transfer heat to the quantification tube 20. Specifically, in order to facilitate heat transfer, the main housing 1 can be integrally processed by using a material easy to conduct heat. In order to save materials, the main housing 1 can also be processed in different regions, and the region near the side wall of the quantification tube 20 is processed by using a material easy to conduct heat.

[0044] Further, the liquid storage channel 23 is provided with a liquid supplement piston 24, and a pressure area 26 is arranged below the liquid supplement piston 24. A liquid storage tube 27 is arranged below the pressure area 26, and the liquid storage tube 27 stores a buffer solution. Specifically, the pressure area 26 and the liquid storage tube 27 are both circular in cross section (i.e. the interface in the horizontal direction, the same below), and the cross-sectional area of the liquid storage tube 27 is smaller than that of the pressure area 26. The bottom surface of the pressure area 26 can define the lowest position of the downward movement of the liquid storage channel 23. The cross-sectional area of the liquid storage tube 27 is set to be smaller than that of the pressure area 26, so as to increase the flow rate of the buffer solution output by the liquid storage tube 27, and facilitate the agitation of the buffer solution downstream to mix well with the liquid or powder reagent downstream. When the adjusting valve 6 is moved to the third position, the liquid storage tube 27 is in communication with the liquid supplement channel 10, and the liquid supplement piston 24 is used to increase the air pressure in the liquid supplement channel 10, so as to inject the liquid reagent in the liquid storage channel 23 into the liquid supplement channel 10. Preferably, the liquid storage tube 27 is arranged close to the side wall of the main housing 1, and the side wall of the main housing 1 close to the liquid storage tube 27 is configured to transfer heat to the liquid storage tube 27. Specifically, the main housing 1 can be integrally machined from a material that is easy to conduct heat. Alternatively, the main housing 1 can be machined in different regions, and the side wall close to the liquid storage tube 27 can be machined from a material that is easy to conduct heat. Similarly, the input channel 12 and the output channel 28 that need to be heated can also be machined from a material that is easy to conduct heat near the side wall thereof, including machining the main housing 1 from a material that is easy to conduct heat, and machining the side wall of the input channel 12 and the output channel 28 from a material that is easy to conduct heat.

[0045] Further, as shown in Figure 11 and Figure 13 , the output channel 28 includes a first channel 29 and a second channel 30 arranged in a spaced manner and extending in the vertical direction. When the adjusting valve 6 is moved to the second position, the liquid injection channel 9 is in communication with the first channel 29, so as to transport the preset volume of the analyte in the dosing tube 20 to the downstream for culture through the first channel 29. When the adjusting valve 6 is moved to the third position, the liquid supplement channel 10 is in communication with the second channel 30, so as to flow the liquid reagent in the liquid storage channel 23 into the second channel 30 through the liquid supplement channel 10, and flow to the downstream to mix with the analyte through the second channel 30.

[0046] Further, as shown in Figure 13As shown, the main housing 1 is provided with a connecting column 2 extending in the vertical direction, and the first channel 29 and the second channel 30 are constructed inside the connecting column 2 and pass through the connecting column 2. The lower part of the connecting column 2 is provided with an amplification tube 3 communicating with the first channel 29 and the second channel 30, and the amplification tube 3 is used for amplifying the to-be-tested substance. Specifically, the amplification tube 3 is made of transparent material, and specifically, it is a PCR tube (Polymerase Chain Reaction Tube, which is a special container used for carrying out polymerase chain reaction in molecular biology experiments). After a preset volume of to-be-tested substance enters the amplification tube 3 through the first channel 29, the reagent and the to-be-tested substance in the amplification tube 3 can be cultured and amplified according to the culture conditions. The culture conditions can be incubation, light irradiation, stirring and addition of liquid reagent, etc. In this embodiment, the first lyophilized reagent is stored in the amplification tube 3, and the second lyophilized reagent is stored in the liquid supplement channel 10. When the liquid reagent passes through the liquid supplement channel 10, it can flush the second lyophilized reagent in the liquid supplement channel 10 and deliver the second lyophilized reagent into the amplification tube 3.

[0047] Further, as shown in Figure 13 The amplification tube 3 and the connecting column 2 are further provided with a storage member 4, the storage member 4 is sleeved on the connecting column 2, and the mouth of the amplification tube 3 is sleeved on the storage member 4. The lower end of the connecting column 2 is provided with an extension tube 31, and the first channel 29 passes through the extension tube 31 in the vertical direction. The storage member 4 is provided with a storage space 5 and a clearance hole 66 arranged in sequence and communicating with each other in the vertical direction, the extension tube 31 is inserted into the clearance hole 66, and the extension tube 31 and the clearance hole 66 are sealed. The storage member 4 is further provided with a third channel communicating with the clearance hole 66, the upper end of the storage space 5 communicates with the second channel 30 and the lower end communicates with the third channel, and the lower end of the third channel communicates with the amplification tube 3. In this embodiment, the first lyophilized reagent is stored in the amplification tube 3, and the second lyophilized reagent can also be stored in the storage space 5. When the liquid reagent passes through the storage space 5, it can flush the second lyophilized reagent into the amplification tube 3. Specifically, the storage of the second lyophilized reagent in the liquid supplement channel 10 or the storage space 5 needs to be selected according to the solubility, fluidity and other physical and chemical properties of the second lyophilized reagent. When a large dose of second lyophilized reagent is needed, the second lyophilized reagent can also be stored in the liquid supplement channel 10 and the storage space 5.

[0048] Further, the amplification tube 3 is provided with a stirring device, which is used to rotate in the alternating magnetic field environment to stir the reagent in the amplification tube 3. Specifically, the stirring device is a magnetic stirrer, and when the amplification tube 3 is placed in the alternating magnetic field, the magnetic stirrer can vibrate in the amplification tube 3 to mix the various reagents in the amplification tube 3.

[0049] Further, as shown in Figure 11 And Figure 13As shown, the upper part of the liquid storage channel 23 is provided with a piston pressing block 25, which is detachably connected with the main shell 1. The piston pressing block 25 is used to block the liquid supplement piston 24 from separating from the liquid supplement channel 10. The upper end of the quantitative channel 19 is provided with a liquid outlet, which is covered with a sealing cover 22. The piston pressing block 25 is provided with an extension, the lower surface of which is in pressure contact with the upper surface of the sealing cover 22. After the biological detection is completed, the piston pressing block 25 can be detached from the main shell 1, and the excess to-be-detected substance in the quantitative channel 19 can be taken out, so as to recycle the main shell 1.

[0050] Embodiment 2 Different from embodiment 1, in the present fact example, the number of the input channels 12 of the kit 35 is two, which are the lysis channel 13 and the quantitative channel 19 (as shown in Figure 5 Embodiment 2 omits the liquid storage channel 23, the liquid supplement channel 10 on the regulating valve 6, the storage member 4 and the second channel 30, and can be applied to experiments that do not need to add liquid reagents, supplement reagents or add freeze-dried reagents. For example, in nucleic acid detection, only the to-be-detected substance in the lysis channel 13 is lysed to release nucleic acid fragments, and then a preset volume of the to-be-detected substance is measured by the quantitative tube 20 and is transported into the amplification tube 3 for amplification culture.

[0051] For the kit 35 provided in embodiment 1 and embodiment 2, the present application further provides a regulating module 53 for driving the regulating valve 6 to run along the length direction M thereof. As shown in Figure 3 and Figure 4As shown, specifically, the core module 34 further comprises an adjusting module 53 connected with the adjusting valve 6, the adjusting module 53 being used to drive the adjusting valve 6 to move to a specific position along the length direction M. The adjusting module 53 comprises a mounting bracket 54, a moving bracket 55 and a second driving module 57. The mounting bracket 54 is arranged on the core module 34. The moving bracket 55 is connected with the mounting bracket 54 and can reciprocate along the length direction M relative to the mounting bracket 54. The moving bracket 55 is provided with two pin columns 56, and the end faces of the pin columns 56 respectively abut the two end faces of the adjusting valve 6. The second driving module 57 is arranged on the mounting bracket 54, and the driving end of the second driving module 57 is connected with the moving bracket 55. The second driving module 57 is used to drive the moving bracket 55 to reciprocate along the length direction M. Specifically, the mounting bracket 54 can be directly connected with the core module 34 or indirectly connected with the core module 34. Any connection mode that can keep the mounting bracket 54 and the core module 34 in a relative position fixedly unchanged is within the protection scope of the present application. In the embodiment, the core module 34 further comprises a mounting back plate 82, the mounting back plate 82 being fixedly connected with the mounting plate 46. The mounting plate 46 is horizontally placed, and the mounting back plate 82 is vertically placed. The cracking module 47 and the mounting bracket 54 are both arranged on the mounting back plate 82. It is worth mentioning that the mounting bracket 54 is provided with a second guide seat 83, and the second guide seat 83 is provided with a second guide groove 84 extending along the length direction M. The moving bracket 55 is provided with a second guide rail 85 extending along the length direction M, the second guide rail 85 being arranged in the second guide groove 84 and being capable of reciprocating along the length direction M in the second guide groove 84. The outer wall of the second guide rail 85 is in close contact with the inner wall of the second guide groove 84, and grease can be added between the outer wall of the second guide rail 85 and the inner wall of the second guide groove 84 to reduce the friction between the second guide rail 85 and the second guide groove 84.

[0052] Further, the second driving module 57 is a cylinder, an electric cylinder, an electric push rod or a linear stepping motor, and other power mechanisms capable of driving the moving bracket 55 to move linearly.

[0053] Further, as shown in FIG. 1, the adjusting valve 6 is arranged on the cracking module 47, and the cracking module 47 is arranged on the mounting back plate 82. The cracking module 47 is provided with a first guide seat 71, and the first guide seat 71 is provided with a first guide groove 72 extending along the length direction M. The adjusting valve 6 is provided with a first guide rail 73 extending along the length direction M, the first guide rail 73 being arranged in the first guide groove 72 and being capable of reciprocating along the length direction M in the first guide groove 72. The outer wall of the first guide rail 73 is in close contact with the inner wall of the first guide groove 72, and grease can be added between the outer wall of the first guide rail 73 and the inner wall of the first guide groove 72 to reduce the friction between the first guide rail 73 and the first guide groove 72. Figure 5 、 Figure 9 、 Figure 11 and Figure 13As shown, the at least one input channel 12 of the kit 35 is provided with a pressurizing piston 58, which is used to drive the pressurizing piston 58 to move towards the delivery channel 7 to drive the liquid in the input channel 12 to flow into the delivery channel 7. The kit 35 provided in Embodiment 1 has two pressurizing pistons 58, which are the feeding piston 16 in the lysis channel 13 and the replenishing piston 24 in the liquid storage channel 23. The kit 35 provided in Embodiment 2 has one pressurizing piston 58, which is the feeding piston 16. In other embodiments, there can be more pressurizing pistons 58, each of which is used to push the reagent in the input channel 12 to which it belongs into the delivery channel 7.

[0054] Corresponding to the pressurizing pistons 58 in the kit 35, the detection analyzer further comprises at least one piston driving module 60, the number of the piston driving modules 60 is equal to the number of the pressurizing pistons 58, and each piston driving module 60 corresponds to one pressurizing piston 58. The piston driving module 60 is used to apply a downward pushing force to the pressurizing piston 58. The bottom of the casing 32 is provided with a bottom plate 37, the bottom plate 37 is provided with a rack 59, each piston driving module 60 is arranged on the rack 59 and located above the lysis module 47, and the piston driving module 60 is used to drive the pressurizing piston 58 in the corresponding input channel 12 to move towards the delivery channel 7.

[0055] Further, as shown in Figure 7 The piston driving module 60 comprises a third driving module 61 and a piston pressing column 62. The third driving module 61 is arranged on the rack 59 and located above the movement module 34, the driving end of the third driving module 61 faces downward and can move reciprocatingly in the vertical direction. The piston pressing column 62 is connected to the driving end of the third driving module 61, and the projection of the piston pressing column 62 in the vertical direction falls on the upper end surface of the corresponding pressurizing piston 58. When the third driving module 61 drives the piston pressing column 62 to move downward, the third driving module 61 can apply a downward pressure to the pressurizing piston 58 to push the pressurizing piston 58 downward.

[0056] Further, as shown in Figure 7As shown, the piston driving module 60 further comprises a limiting block 63 and a block spring 64. The limiting block 63 is arranged at the bottom end of the piston pressing column 62, and the projection of the limiting block 63 in the vertical direction falls on the top surface of the main shell 1 of the reagent box 35. The block spring 64 is arranged between the limiting block 63 and the piston pressing column 62, and the block spring 64 and the limiting block 63 are used to apply a downward pressure to the main shell 1 of the reagent box 35, so that the reagent box 35 is pressed against and fitted with the contact surface inside the movement core module 34. Specifically, after the reagent box 35 is placed inside the movement core module 34, the ejection module 36 drives the movement core module 34 to move to the inside of the shell, and is located below the piston driving module 60. The third driving module 61 drives the piston pressing column 62 to move the limiting block 63 downward, so that the limiting block 63 is in contact with the upper surface of the main shell 1 of the reagent box 35. Then, the piston pressing column 62 continues to move downward, and before the piston pressing column 62 is in contact with the pressing piston 58, the block spring 64 is compressed and applies a downward pressure to the reagent box 35, so that the amplification tube 3 is fitted with the inner wall of the containing cavity 65, and the outer wall of the lysis section 15 is fitted with the first heating surface, and the outer wall of the quantitative tube 20 is fitted with the second heating surface. When it is necessary to push the pressing piston 58 to move downward, the piston driving module 60 continues to move downward to compress the block spring 64 to the limit position, and at the same time, the pressing piston 58 is moved downward to increase the pressure in the lysis section 15, and the to-be-tested substance is pushed into the quantitative tube 20. It can be understood that the third driving module 61 is a cylinder, an electric cylinder, an electric push rod or a linear stepper motor, and other power mechanisms capable of driving the moving support 55 to move linearly. Similarly to the second driving module 57, any power mechanism capable of driving the piston pressing column 62 to move linearly is within the protection scope of the present application.

[0057] Further, as shown in Figure 5 and Figure 6 , the amplification module 50 further comprises an optical detection assembly 68 and an excitation light assembly 69, and the detection end of the optical detection assembly 68 and the emission end of the excitation light assembly 69 both face the amplification tube 3; the number of the optical detection assembly 68 is two, and the two optical detection assemblies 68 are oppositely arranged; the excitation light assembly 69 is arranged on the same side of the two optical detection assemblies 68, and the extension line of the emission end is perpendicular to the extension line of each detection end. Specifically, as shown in Figure 5 , the extension line of the emission end of the excitation light assembly 69 extends along a first direction N, and the extension line of the optical detection assembly 68 extends along a length direction M, and the first direction N is perpendicular to the length direction M.

[0058] Further, as shown in Figure 5 , the outer wall of the amplification module 50 is provided with two optical detection channels 67 extending along the length direction M Figure 5The two optical detection channels on both sides of the amplification module 50 are coaxial with an excitation light channel 70 extending along the first direction N, and the excitation light channel 70 is perpendicular to the optical detection channels. The bath block 52 is provided with a corresponding accommodation hole 66 corresponding to each optical detection channel and excitation light channel 70; the optical detection channels and the excitation light channel 70 are in communication with the accommodation cavity 65 through the corresponding accommodation hole 66. Each optical detection channel and excitation light channel is coaxial with the corresponding accommodation hole 66. The detection end is inserted into the optical detection channel, and the emission end is inserted into the excitation light channel 70.

[0059] Further, as shown in Figure 5 and Figure 6 The machine core module 34 further includes a mixing assembly, and the mixing assembly includes a plurality of electromagnets 73. The outer wall of the amplification module 50 is further provided with a plurality of mounting holes, and each electromagnet 73 is mounted on the corresponding mounting hole. The amplification tube 3 has a magnetic stirring rod, and each electromagnet 73 is used to provide an alternating magnetic field to make the magnetic stirring rod rotate in the amplification tube 3. The mounting hole is arranged at intervals with the detection hole.

[0060] Preferably, in some embodiments, the mixing assembly includes at least two electromagnets 73, which are arranged at intervals along the circumference of the mounting hole, preferably symmetrically along the circumference of the mounting hole. The so-called symmetric arrangement means that the at least two electromagnets 73 are uniformly distributed along the circumference of the mounting hole. By cooperating with the plurality of electromagnets 73 to apply an alternating magnetic field to the region where the to-be-detected object is located, the motion path of the magnetic stirring rod in the to-be-detected object can be controlled to facilitate more thorough mixing of the to-be-detected object.

[0061] Illustratively, in some embodiments, the mixing assembly includes three electromagnets 73, which are uniformly distributed along the circumference of the corresponding mounting hole. When performing the mixing operation, the three electromagnets 73 are sequentially energized, so that the three electromagnets 73 sequentially generate magnetic fields and sequentially attract the magnetic stirring rod in the to-be-detected object, so that the magnetic stirring rod in the to-be-detected object can make rotational motion along the circumference of the mounting hole, thereby improving the mixing effect and efficiency.

[0062] It can be understood that in other optional embodiments, the number of electromagnets 73 is not limited to three, for example, the number of electromagnets 73 can also be four, five, six, etc.

[0063] Further, as shown in Figure 1 The monitoring analyzer further includes a warehouse door 80 connected with the ejection module 36 or the machine core module 34. When the machine core module 34 moves to the inside of the machine shell 32, the warehouse door 80 covers the ejection port 33.

[0064] While the application has been illustrated and described in connection with certain preferred embodiments thereof, it will be readily apparent to those of ordinary skill in the art that various changes in form and detail can be made therein without departing from the spirit and scope of the application. It is intended to encompass all such changes and alterations in the details thereof.

Claims

1. A detection analyzer based on a kit, characterized in that, The detection analyzer comprises: A housing, an outer wall of which is provided with an outlet facing a first direction; A core module is provided inside the housing, and is used to receive the test kit and perform pre-processing and detection on the test object in the test kit; The outlet module is arranged inside the housing and connected to the core module; the outlet module can reciprocate along the first direction relative to the outlet port to drive the core module to enter and exit the outlet port.

2. The detection analyzer according to claim 1, wherein: The bottom of the housing is provided with a bottom plate, and the bottom plate is detachably connected to the housing. The outgoing module includes: a first guide seat, disposed on the bottom plate, wherein the first guide seat is provided with a first guide groove extending along the first direction; a first guide rail, disposed at the bottom of the movement module and extending along the first direction, the first guide rail being inserted into the first guide groove, the outer wall of the first guide rail being adapted to the inner wall of the first guide groove, and the first guide rail being capable of reciprocatingly sliding relative to the first guide seat along the first direction; The first driving module is provided on the bottom plate. The driving end of the first driving module is connected to the movement module. The first driving module is used to drive the movement module to move back and forth along the first direction.

3. The detection analyzer according to claim 1 or 2, characterized in that: The kit is provided with a lysis section and an amplification tube spaced apart in a vertical direction. The lysis section is used to accommodate the analyte and pre-treat the analyte, and the amplification tube is used to amplify the pre-treated analyte under preset conditions.

4. The detection analyzer according to claim 3, wherein: The core module includes: A mounting plate, provided at the bottom of the movement module; A cracking module is provided above the mounting plate, wherein a cracking chamber is provided inside the cracking module, and a first temperature controller is provided inside the cracking chamber; an amplification module, disposed on the mounting plate and connected to the bottom of the lysis module; an amplification chamber is provided inside the amplification module, the amplification chamber is communicated with the lysis chamber, and a second temperature controller is provided inside the amplification chamber; When the test kit is housed in the core module, the lysis section is located in the lysis chamber, and the amplification tube is located in the amplification chamber; and the first temperature controller is in contact with the outer wall of the lysis section, and the second temperature controller is in contact with the outer wall of the amplification tube.

5. The detection analyzer according to claim 4, characterized in that: The test kit also includes a quantitative tube arranged in parallel with the cracking section. When the test kit is stored in the movement module, the quantitative tube is located in the cracking chamber. A third temperature controller is provided in the cracking chamber. The third temperature controller is in contact with the outer wall of the quantitative tube. The third temperature controller is used to control the temperature in the quantitative tube.

6. The detection analyzer according to claim 5, characterized in that: The test kit includes multiple input channels and at least one output channel, and a regulating valve arranged between the input channels and the output channels; the lysis section is part of one of the input channels, the quantitative tube is part of another input channel, and the amplification tube is connected to the output end of the output channel; multiple delivery channels are provided inside the regulating valve, and the regulating valve can move back and forth along its own length direction; the regulating valve is configured so that when it moves to a specific position, the corresponding delivery channel can connect two of the input channels, or can connect one of the input channels with one of the output channels.

7. The detection analyzer according to claim 6, characterized in that: The movement module further includes a regulating module connected to the regulating valve, the regulating module being used to drive the regulating valve to move to the specific position along the length direction, and the regulating module including: A mounting bracket is provided on the movement module; a movable bracket connected to the mounting bracket and capable of reciprocating relative to the mounting bracket along the longitudinal direction; the movable bracket is provided with two pins, the end faces of each pin respectively abutting against the two end faces of the regulating valve; The second driving module is provided on the mounting bracket, the driving end of the second driving module is connected to the movable bracket, and the second driving module is used to drive the movable bracket to reciprocate relative to the mounting bracket along the length direction.

8. The detection analyzer according to claim 6, characterized in that: A pressurizing piston is provided in at least one of the input channels, and the pressurizing piston is used to drive the pressurizing piston to move toward the delivery channel, so as to drive the liquid in the input channel to flow into the delivery channel.

9. The detection analyzer according to claim 8, characterized in that: A base plate is provided at the bottom of the casing, a frame is provided on the base plate, and at least one piston driving module is provided on the frame. The piston driving module is used to drive the pressurizing piston in the corresponding input channel to move toward the delivery channel.

10. The detection analyzer according to claim 9, characterized in that: The piston drive module comprises: A third driving module is provided on the frame and located above the core module, with a driving end of the third driving module facing downward and capable of reciprocating in a vertical direction; A piston pressure column is connected to the driving end of the third driving module; the projection of the piston pressure column in the vertical direction falls on the upper end surface of the pressurizing piston; A limiting pressure block is provided at the bottom end of the piston column, wherein the projection of the limiting pressure block in the vertical direction falls on the top surface of the main housing of the reagent box; The pressure block spring is arranged between the limit pressure block and the piston pressure column. The pressure block spring and the limit pressure block are used to apply downward pressure to the main shell of the reagent box, so that the reagent box is pressed against and fits the contact surface inside the movement module.

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