Mesenchymal stem cell culture and separation device

Abstract

The invention discloses a mesenchymal stem cell culture and separation device, and relates to the technical field of cell separation. Comprising a frame, a culture dish and two first telescopic pieces are arranged on a bottom plate of the frame, a second telescopic piece is arranged at the bottom of a top plate of the frame, a push plate assembly is detachably connected to the second telescopic piece, and a plurality of push plates are evenly arranged below the push plate assembly; a culture block is placed on the culture dish, and a plurality of culture cavities matched with the push plates are formed in the culture block; sliding grooves are formed in two side plates of the frame, sliding blocks are arranged in the sliding grooves in a sliding mode, the two sliding blocks are fixedly connected with the tops of the two first telescopic pieces correspondingly, and elastic columns are arranged on the sides, facing the culture dishes, of the sliding blocks; inserting holes matched with the two elastic columns are formed in the two sides of the culture block. The multi-cavity culture block is adopted to increase the adsorption area, a large number of cells are convenient to culture, meanwhile, the push plate assembly is matched to facilitate separation, and the structure is simple; the culture blocks and the push plate assembly are detachable, so that cleaning is convenient.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cell separation, in particular to a mesenchymal stem cell culture and separation device. BACKGROUND

[0002] Mesenchymal stem cells (MSCs) are a kind of multipotent stem cells belonging to mesoderm, mainly existing in connective tissue and interstitial tissue of organs. They have characteristics such as multi-directional differentiation potential, hematopoietic support, promoting stem cell implantation, immune regulation and self-replication. Mesenchymal stem cells have made significant breakthroughs in clinical applications to solve various blood system diseases, cardiovascular diseases, cirrhosis, nervous system diseases, knee meniscus partial resection injury repair, autoimmune diseases and other aspects, and have saved more lives of patients. In addition, mesenchymal stem cells have long-term development prospects in the repair of the nervous system and more.

[0003] At present, the separation of mesenchymal stem cells is mostly carried out by the adhesion method. For example, the separation devices disclosed in Chinese patents CN116716163A and CN113881565A use multiple culture plates to increase the adsorption area inside the incubator during cell culture, which is convenient for large-scale cell culture. However, the above structure is complex and not convenient to clean. SUMMARY

[0004] The main purpose of the present application is to provide a mesenchymal stem cell culture and separation device to solve the above problems.

[0005] To achieve the above purpose, the present application provides a mesenchymal stem cell culture and separation device, which comprises a frame, a culture dish and two first telescopic members are arranged on the bottom plate of the frame, a drain pipe is arranged on the culture dish, and an electromagnetic valve is arranged on the drain pipe; a second telescopic member is arranged at the bottom of the top plate of the frame, a push plate assembly is detachably connected to the second telescopic member, and a plurality of push plates are uniformly arranged below the push plate assembly; a culture block is placed on the culture dish, and a plurality of culture cavities matched with the push plates are arranged on the culture block; a sliding groove is arranged on each side plate of the frame, a sliding block is slidingly arranged in the sliding groove, and the top of each first telescopic member is fixedly connected to the sliding block on the side facing the culture dish; and an elastic column is arranged on the side of the sliding block facing the culture dish.

[0006] Further, a vibration assembly is arranged on the push plate assembly.

[0007] Further, the vibration assembly uses a vibrator or a vibration motor.

[0008] Further, the length of the push plate is greater than the length of the culture cavity.

[0009] Further, the top of the push plate assembly is provided with a T-shaped clamping groove, and the end of the second telescopic member is provided with a clamping plate matched with the T-shaped clamping groove.

[0010] Further, the elastic column comprises a sleeve and a sliding column slidingly arranged in the sleeve, and the sleeve is provided with a spring.

[0011] Further, the sliding column is provided with a limiting plate.

[0012] Further, the first telescopic member and the second telescopic member are air cylinders, electric cylinders or hydraulic cylinders.

[0013] The present application has the following beneficial effects:

[0014] The present application adopts the culture block in the form of multiple cavities to increase the adsorption area, facilitates the culture of a large number of cells, and separates the cells conveniently with the push plate assembly, and the culture block and the push plate assembly are in a detachable form, facilitating cleaning. BRIEF DESCRIPTION OF DRAWINGS

[0015] Fig. 1 It is a whole schematic view of the mesenchymal stem cell culture and separation device.

[0016] Fig. 2 It is a sectional view of the mesenchymal stem cell culture and separation device.

[0017] Wherein, 1-frame; 2-sliding groove; 3-elastic column; 4-sliding block; 5-first telescopic member; 6-culture dish; 7-drainage pipe; 8-solenoid valve; 9-culture plate; 10-culture block; 11-push plate; 12-push plate assembly; 13-second telescopic member; 14-vibration assembly; 15-clamping plate; 16-T-shaped clamping groove; 17-jack; 18-culture cavity; 19-sliding column; 20-limiting plate; 21-sleeve; 22-spring. DETAILED DESCRIPTION

[0018] In order to achieve the above-mentioned purposes and effects, the technical means and structure adopted by the present application are described in detail in combination with the preferred embodiments of the present application with reference to the accompanying drawings.

[0019] As Figs. 1-2As shown, the mesenchymal stem cell culture separation device provided in the application comprises a frame 1, a culture dish 6 and two first telescopic members 5 are arranged on the bottom plate of the frame 1, a drain pipe 7 is arranged on the culture dish 6, and an electromagnetic valve 8 is arranged on the drain pipe 7; a second telescopic member 13 is arranged at the bottom of the top plate of the frame 1, a push plate assembly 12 is detachably connected to the second telescopic member 13, and a plurality of push plates 11 are uniformly arranged below the push plate assembly 12; a culture block 10 is placed on the culture dish 6, a plurality of culture cavities 18 matched with the push plates 11 are arranged on the culture block 10, and the length of the push plates 11 is greater than the length of the culture cavities 18; a sliding groove 2 is arranged on each side plate of the frame 1, a sliding block 4 is slidably arranged in the sliding groove 2, and the top of each first telescopic member 5 is fixedly connected with the sliding block 4; and a resilient column 3 is arranged on the side of the sliding block 4 facing the culture dish 6; and a plug hole 17 matched with the two resilient columns 3 is arranged on each side of the culture block 10.

[0020] In use, the push plates 11 of the push plate assembly 12 are inserted into the culture cavities 18 of the culture block 10, then the push plate assembly 12 is installed on the second telescopic member 13, and the resilient columns 3 are inserted into the plug holes of the culture block 10. The culture solution containing cells is placed in the culture dish 6, the two first telescopic members 5 are controlled to drive the culture block 10 to rise, so that the distance between the bottom of the culture block 10 and the culture dish 6 is 0.5 mm, then the second telescopic member 13 is controlled to drive the push plate assembly 12 to rise, the push plates 11 rise to generate negative pressure in the culture cavities 18, and then the culture solution in the culture dish 6 is sucked, when the second telescopic member 13 rises to the highest point, the first telescopic member 5 is controlled to descend, so that the bottom of the culture block 10 is attached to the culture dish 6. After a certain period of culture, the mesenchymal stem cells are attached to the inner wall of the culture cavity 18. After the attachment is completed, the two first telescopic members 5 are controlled to drive the culture block 10 to rise, so that the distance between the bottom of the culture block 10 and the culture dish 6 is 5-10 mm, the culture solution in the culture cavities 18 is discharged to the culture dish 6, and at the same time, the electromagnetic valve 8 is opened to discharge the culture solution in the culture dish 6. After the discharge is completed, the second telescopic member 13 is controlled to drive the push plate assembly 12 to descend, so that the push plates 11 scrape off the mesenchymal stem cells on the inner wall of the culture cavities 18 and fall onto the culture dish. As a preferred embodiment, the culture plate 9 can be placed in the culture dish in advance, and the mesenchymal stem cells can fall onto the culture plate 9, which is convenient for taking out.

[0021] In cleaning, the resilient columns 3 can be moved to both sides, the push plate assembly 12 and the culture block 10 can be integrally moved forward, the push plate assembly 12 and the culture block 10 can be integrally detached, and then the push plate assembly 12 can be taken out of the culture block 10, so that the cleaning can be performed, which is simple and convenient.

[0022] In another embodiment, the push plate assembly 12 is provided with a vibration assembly 14, which is a vibrator or a vibration motor. The cells attached to the lower end of the push plate 11 are detached by vibration of the vibration assembly 14.

[0023] In another embodiment, the top of the push plate assembly 12 is provided with a T-shaped clamping slot 16, and the end of the second telescopic member 13 is provided with a clamping plate 15 matching the T-shaped clamping slot 16, so as to realize quick assembly and disassembly.

[0024] In another embodiment, the elastic column 3 comprises a sleeve 21 and a sliding column 19 slidingly arranged in the sleeve 21, the sleeve 21 is provided with a spring 22, and the sliding column is provided with a limiting plate 20. When disassembling, the sliding column 19 is detached from the insertion hole 17 by pushing the limiting plate to both sides, so as to disassemble. By using the spring arrangement, the culture block 10 can move left and right, which facilitates the vibration assembly 14 to transmit the vibration to the lower end of the push plate 11.

[0025] In another embodiment, the first telescopic member 5 and the second telescopic member 13 can be air cylinders, electric cylinders or hydraulic cylinders.

[0026] The above description is only the preferred embodiment of the present application, not all embodiments, anyone should know that the structural changes made under the inspiration of the present application, any technical solution with the same or similar to the present application, all belong to the protection scope of the present application.

Claims

1. A mesenchymal stem cell culture and separation device, characterized in that: The invention comprises a frame, a culture dish and two first telescopic parts are provided on the bottom plate of the frame, a drainage pipe is provided on the culture dish, and a solenoid valve is provided on the drainage pipe; a second telescopic part is provided on the bottom of the top plate of the frame, a push plate assembly is detachably connected to the second telescopic part, and a plurality of push plates are evenly provided below the push plate assembly; a culture block is placed on the culture dish, and a plurality of culture cavities adapted to the push plates are provided on the culture block; a slide groove is provided on both side plates of the frame, a slider is provided for sliding in the slide groove, and the two sliders are fixedly connected to the tops of the two first telescopic parts respectively, and an elastic column is provided on the side of the slider facing the culture dish; and a socket adapted to the two elastic columns is provided on both sides of the culture block.

2. The mesenchymal stem cell culture and separation device according to claim 1, wherein: A vibration component is provided on the push plate component.

3. The mesenchymal stem cell culture and separation device according to claim 2, characterized in that: The vibration component adopts a vibrator or a vibration motor.

4. The mesenchymal stem cell culture and separation device according to claim 1, wherein: The length of the push plate is greater than the length of the culture chamber.

5. The mesenchymal stem cell culture and separation device according to claim 1, wherein: A T-shaped slot is provided on the top of the push plate assembly, and a card plate adapted to the T-shaped slot is provided on the end of the second telescopic member.

6. The mesenchymal stem cell culture and separation device according to claim 1, wherein: The elastic column comprises a sleeve and a sliding column slidably arranged in the sleeve, and a spring is arranged in the sleeve.

7. The mesenchymal stem cell culture and separation device according to claim 6, characterized in that: A limit plate is provided on the sliding column.

8. The mesenchymal stem cell culture and separation device according to claim 1, wherein: The first telescopic member and the second telescopic member are pneumatic cylinders, electric cylinders or hydraulic cylinders.

Images