Fish scale collagen peptide extraction and filtration device and preparation method

Abstract

The application discloses a fish scale collagen peptide extraction and filtration device and a preparation method. The device comprises a tank body with an open bottom. A transonic vortex airflow field generating block is rotatably sleeved to the inner wall of the tank body near the top. A spiral electric field assembly is installed at the axis below the transonic vortex airflow field generating block. A material atomization assembly is installed at the top of the spiral electric field assembly. A wind vibration assembly is installed at the inner wall of the bottom port of the tank body. A cylindrical filter membrane is sleeved to the outer periphery of the spiral electric field assembly and connected between the wind vibration assembly and the material atomization assembly. An air cavity is arranged at the top of the tank body. A ceramic tube penetrates the middle part of the spiral electric field assembly. The application relates to the technical field of protein peptide extraction. The application solves the problems of low separation efficiency, difficult balance between target peptide purity and recovery rate, incomplete centrifugal separation, easy blocking and damage of membrane filtration, and poor synergy of each link in traditional fish scale collagen peptide extraction and filtration.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of protein peptide extraction, in particular to a fish scale collagen peptide extraction and filtration device and a preparation method. BACKGROUND

[0002] Collagen peptides have the characteristics of small molecules, easy absorption, and excellent biological activity, and the application demand in the fields of food health care, cosmetics, biological medicine, etc. is continuously growing. Fish scales, as a large amount of waste generated by the aquatic processing industry, are rich in natural collagen inside, and are widely available and low-cost, making them an ideal raw material for large-scale extraction of collagen peptides. It is of great significance to promote the resource utilization of aquatic waste and the development of collagen peptide industry.

[0003] Currently, the extraction and filtration of fish scale collagen peptides generally rely on the traditional process route of "enzymolysis-centrifugation-membrane filtration": first, the fish scales are subjected to enzymolysis treatment by protease to obtain a mixed system containing target collagen peptides and various impurities (such as unenzymolyzed macromolecular proteins, gums, mucopolysaccharides, etc.); then, preliminary separation is performed by centrifugal equipment to try to remove part of the macromolecular impurities; finally, the target peptide is purified by flat membrane or spiral membrane filtration. However, this traditional process route has long been limited by the core bottlenecks of "low separation efficiency" and "difficulty in balancing target peptide purity and recovery rate" in practical application, and it is difficult to meet the needs of the industry for high-quality and high-efficiency production. The specific problems are as follows:

[0004] From the perspective of centrifugal separation, fish scale enzymolysis liquid has high viscosity due to the presence of gum, mucopolysaccharide and other components, which makes the traditional centrifugal equipment face significant limitations in the separation process. On the one hand, the viscous liquid increases the resistance of centrifugal separation, resulting in longer time for the equipment to complete one separation operation, and the overall processing efficiency is low; on the other hand, small molecule target collagen peptides are easily wrapped by macromolecular impurity particles in the system, and it is difficult to achieve complete physical separation. A large amount of impurities remain in the liquid after preliminary centrifugation, which not only cannot reduce the burden of the subsequent purification process, but also has a negative impact on the subsequent filtration effect.

[0005] In the subsequent membrane filtration link, the "passive penetration" mode adopted by the traditional process further aggravates the technical pain points. The core of membrane filtration relies on the liquid to pass through the membrane pores under the action of external pressure to realize separation, but due to incomplete centrifugal separation in the early stage, impurities remaining in the liquid are easy to adhere to the membrane surface and block the membrane pores, resulting in a significant decrease in filtration efficiency with running time, and frequent shutdown is required for cleaning or replacing the membrane module, which seriously disrupts the continuous production process. More importantly, there is a difficult to reconcile contradiction between the target peptide purity and the recovery rate in the traditional membrane filtration: if a membrane assembly with a smaller pore size is selected to improve the purity of the target peptide, it will cause part of the small molecule target peptide to be intercepted by the membrane, resulting in a significant decrease in recovery rate; if a membrane assembly with a larger pore size is selected to reduce the loss of target peptides, part of the small molecule impurities will penetrate the membrane pores, resulting in the final product purity being difficult to meet the quality requirements of high-end markets for high-purity collagen peptides.

[0006] In addition, the synergy of the two core links of centrifugation and membrane filtration in the traditional process is insufficient, further amplifying the efficiency and quality problems. Incomplete centrifugal separation of the liquid directly enters the membrane filtration link, accelerating the membrane pore blockage and membrane assembly loss, shortening the service life of the membrane; and to make up for the lack of separation effect, some production processes need to additionally increase steps such as "secondary centrifugation" or "multi-stage membrane filtration", which not only prolongs the overall production cycle, but also causes additional loss of target collagen peptides due to multiple treatments, forming a vicious cycle of "low efficiency-poor purity-reduced recovery".

[0007] In summary, the traditional fish scale collagen peptide extraction and filtration process cannot break through the core bottlenecks of "low separation efficiency" and "difficulty in balancing target peptide purity and recovery rate" due to incomplete centrifugal separation, passive and inefficient membrane filtration, and poor synergy between links, which seriously restricts the high-quality development of the fish scale collagen peptide industry, and a new type of extraction and filtration technology scheme that can realize efficient separation and balance target peptide purity and recovery rate is urgently needed to solve the long-standing technical pain points in the industry. SUMMARY

[0008] In order to solve the problems of low separation efficiency, difficulty in balancing target peptide purity and recovery rate, incomplete centrifugal separation, membrane filtration prone to blockage and loss, and poor synergy between links in the traditional fish scale collagen peptide extraction and filtration, the purpose of the present application is to provide a fish scale collagen peptide extraction and filtration device and preparation method.

[0009] In order to achieve the above object, the technical scheme adopted by the present application is as follows: a fish scale collagen peptide extraction and filtration device, comprising a tank body with an open bottom, a transonic vortex airflow field generating block rotatably sleeved on the inner wall of the tank body near the top, a spiral electric field assembly installed at the axis below the transonic vortex airflow field generating block, a material atomization assembly installed on the top of the spiral electric field assembly, a wind vibration assembly installed on the inner wall of the bottom port of the tank body, a cylindrical filter membrane sleeved on the outer periphery of the spiral electric field assembly connected between the wind vibration assembly and the material atomization assembly, an air cavity provided at the top inside the tank body, a ceramic tube penetrating the middle part of the spiral electric field assembly, and the ceramic tube vertically upwardly penetrates the top of the transonic vortex airflow field generating block.

[0010] Preferably, the top of the tank body is fixedly connected with a motor, the output end of the motor vertically downwardly penetrates the top of the tank body in a sealed manner, and the output end is fixedly connected with a plurality of connection rods arranged in an annular array, the bottom of the connection rod is fixedly connected with the top of the transonic vortex airflow field generating block, a plurality of arc-shaped grooves are vertically and horizontally formed on the outer wall of the transonic vortex airflow field generating block, and the transonic vortex airflow field generating block is a circular block.

[0011] Preferably, the spiral electric field assembly comprises two spiral electrode bands fixedly sleeved on the outer wall of the ceramic tube, forming a double spiral electrode; a sleeve is sleeved on the outer periphery of the two spiral electrode bands, the ceramic tube is fixedly connected at both ends of the sleeve, the ceramic tube is an aluminum nitride ceramic tube, the cylindrical filter membrane is sleeved on the outer periphery of the sleeve, the outer wall of the sleeve near the bottom is fixedly connected with a first connection rod, the end of the first connection rod away from the sleeve is fixedly connected with the inner wall of the tank body, the outer periphery of the sleeve near the bottom is sleeved with a discharge port, the first connection rod is fixedly connected with the outer wall of the discharge port, and the inner wall of the discharge port near the top is fixedly sleeved with the outer wall of the cylindrical filter membrane near the bottom.

[0012] Preferably, the material atomization assembly comprises an annular cavity fixedly sleeved on the top outer wall of the sleeve, the annular cavity is connected with a fish scale enzyme solution supply pump, the bottom of the annular cavity is fixedly installed with a plurality of ultrasonic atomization nozzles arranged in an annular array, for atomizing the viscous fish scale enzyme solution into micron-sized droplets through ultrasonic high-frequency vibration; and the plurality of ultrasonic atomization nozzles are located on the outer periphery of the cylindrical filter membrane.

[0013] Preferably, the wind vibration assembly comprises a wind vibration cylinder fixedly sleeved on the outer wall of the cylindrical filter membrane, a bottom port of the wind vibration cylinder is arranged close to a top port of the discharge port, an outer wall close to the bottom of the wind vibration cylinder is fixedly connected with a plurality of vibration isolators arranged in an annular array, one end of the vibration isolators away from the wind vibration cylinder is fixedly connected with a second connecting rod, one end of the second connecting rod away from the vibration isolators is fixedly connected with the inner wall of the tank body, and an outer wall close to the top of the wind vibration cylinder is fixedly sleeved with a horn-shaped material guide cover; the wind vibration assembly further comprises a first annular plate rotatably sleeved on the inner wall of the tank body, the inner wall of the first annular plate is fixedly connected with a plurality of impeller blades arranged in an annular array, one end of the plurality of impeller blades away from the first annular plate is fixedly connected with a second annular plate, the second annular plate is located below the bottom port edge of the horn-shaped material guide cover, the inner wall of the second annular plate is fixedly connected with a plurality of rectangular fan plates arranged in an annular array, and an outer wall of the wind vibration cylinder below the horn-shaped material guide cover is fixedly connected with a plurality of elastic steel sheets arranged in an annular array; and the top of the first annular plate and the second annular plate close to the side of the impeller blades is arranged in an inverted bevel.

[0014] Preferably, the gap between the inner wall of the cylindrical filter membrane and the outer wall of the sleeve is 2-5 mm, and the cylindrical filter membrane is in a tension state.

[0015] A fish scale collagen peptide extraction and filtration preparation method comprises the following steps:

[0016] S1, the spiral electric field assembly applies a pulsed electric field, the annular cavity is connected with a fish scale enzymatic solution supply pump, and the bottom port of the ceramic tube is connected with a cooling gas compressor to provide cooling gas with high pressure;

[0017] S2, the cooling gas enters the air cavity through the ceramic tube, and at the same time, the transonic vortex air flow field generating block rotates, the high-pressure gas is discharged from the bottom port of the arc-shaped groove in a transonic state under the assistance of the transonic vortex air flow field generating block, and the transonic vortex air flow field is formed;

[0018] S3, the material atomization assembly sprays the viscous fish scale enzymatic solution downward in a mist state, and the liquid drops with large mass are quickly thrown to the inner wall of the tank body, gathered on the wall surface, and flow downward;

[0019] The target collagen peptides with small mass are subjected to small centrifugal force and are dragged by the gas viscous force, and are stayed near the vortex center area;

[0020] S4, the spiral electric field assembly actively drags and transports the liquid drops to the direction of the tank body axis and downward, so that the target peptide liquid drops stayed near the vortex center area pass through the cylindrical filter membrane, are adsorbed on the outer wall of the sleeve and are transported downward at the same time, are discharged from the discharge port, and are collected;

[0021] S5, the heat generated by the spiral electric field assembly can be taken away by the ceramic tube cooling gas, and at the same time, the cooling gas entering the tank body can also continuously keep low temperature, so as to ensure the quality of the target peptides.

[0022] S6, the cylindrical filter membrane intercepts impurities escaping from the vortex gas flow, the vortex gas flow passes downward through the wind vibration assembly with the impurities, the wind vibration drives the cylindrical filter membrane to vibrate, impurities adhered to the outer wall of the cylindrical filter membrane are shaken off, and the target peptide is ensured to pass normally.

[0023] Compared with the prior art, the present application has the following beneficial effects:

[0024] 1. The present application realizes the fusion of transonic vortex gas flow field centrifugal separation and helical traveling wave electric field dielectrophoresis traction separation double active separation technology. Firstly, the transonic vortex gas flow field can exert differential centrifugal force on the micron-sized droplets after ultrasonic atomization, quickly separate the impurities with significant mass difference from the target peptide, and enrich the target peptide in the vortex center area. Secondly, the helical traveling wave electric field can generate directional dielectrophoresis traction force on the polarized target peptide droplets, like a "conveyor belt", actively dragging the target peptide to the outer wall of the sleeve and downward, avoiding the separation lag problem caused by "passive penetration" in traditional filtration, and the double mechanism synergistically reduces the mixing time of the target peptide and impurities, effectively improves the separation efficiency, and greatly improves the purity and filtration efficiency of the target collagen peptide.

[0025] 2. The wind vibration assembly of the present application utilizes the kinetic energy of the vortex gas flow to drive the impeller to rotate, and through the cooperation of the rectangular fan plate and the elastic steel sheet, the cylindrical filter membrane is vibrated at a high frequency. On the one hand, it can shake off the impurities adhered to the outer wall of the filter membrane in time, avoiding the decline of filtration efficiency caused by membrane hole blockage; on the other hand, it can bounce the target peptide that is intercepted by the filter membrane and make it pass through the membrane body again, avoiding the loss of target components caused by membrane interception.

[0026] 3. The cooling gas is continuously delivered to the inside of the tank body through the ceramic pipe. The aluminum nitride ceramic pipe not only has excellent insulation performance, but also has a high thermal conductivity, which can quickly absorb the heat generated by the spiral electrode during operation. At the same time, the cooling gas in the tank body maintains a low temperature environment inside the tank body at all times, which can effectively inhibit the denaturation reaction of collagen peptide, avoid the problem of reduced activity of target components caused by traditional heating evaporation or mechanical friction heating, and ensure the biological activity and quality stability of the final product.

[0027] 4. The wind vibration assembly of the present application does not need to be equipped with an additional driving motor, but directly utilizes the kinetic energy of the transonic vortex gas flow to drive the impeller to rotate, realizing the secondary utilization of gas flow energy and saving a lot of energy consumption cost. BRIEF DESCRIPTION OF DRAWINGS

[0028] The present application will be further described in detail below in combination with the drawings and specific embodiments:

[0029] Figure 1 It is a structural schematic diagram of the overall section of the present application;

[0030] Figure 2 Structure diagram of the transonic vortex air flow field generating block of the present application;

[0031] Figure 3 Structure diagram of the spiral electric field assembly of the present application;

[0032] Figure 4 Structure diagram of the material atomization assembly of the present application;

[0033] Figure 5 Structure diagram of the wind vibration assembly of the present application.

[0034] In the figure: 1, tank body; 2, transonic vortex air flow field generating block; 201, motor; 202, connecting rod; 203, arc-shaped slot; 3, spiral electric field assembly; 301, spiral electrode belt; 302, sleeve; 303, first connecting rod; 304, discharge port; 4, material atomization assembly; 401, annular cavity; 402, ultrasonic atomization nozzle; 5, wind vibration assembly; 501, wind vibration cylinder; 502, vibration isolator; 503, second connecting rod; 504, horn-shaped material guide cover; 505, first annular plate; 506, impeller blade; 507, second annular plate; 508, rectangular sector plate; 509, elastic steel sheet; 6, cylindrical filter membrane; 7, air cavity; 8, ceramic tube. DETAILED DESCRIPTION

[0035] The following specific embodiments illustrate the embodiments of the present application, and those skilled in the art can easily understand other advantages and effects of the present application from the content disclosed in the specification.

[0036] Please refer to Figures 1 to 5 It should be understood that the structures, proportions, sizes, etc. shown in the drawings attached to the specification are only used to illustrate the content disclosed in the specification for those skilled in the art to understand and read, and are not used to limit the defined conditions under which the present application can be implemented, so they do not have technical significance. Any modification of the structure, change of the proportional relationship or adjustment of the size, without affecting the effects that can be produced by the present application and the purposes that can be achieved, should still fall within the scope of the technical content disclosed by the present application. At the same time, the terms such as "upper", "lower", "left", "right", "middle" and "one" used in the specification are only for the convenience of clear description, and are not used to limit the scope in which the present application can be implemented, and the change or adjustment of the relative relationship, without substantially changing the technical content, is also considered as the scope in which the present application can be implemented.

[0037] The technical scheme is provided by the application: the embodiment provides a fish scale collagen peptide extraction and filtration device, which comprises a tank body 1 provided with an open bottom, a transonic vortex airflow field generating block 2 is rotatably sleeved to the inner wall of the tank body 1 close to the top, a spiral electric field assembly 3 is installed at the axis below the transonic vortex airflow field generating block 2, a material atomization assembly 4 is installed at the top of the spiral electric field assembly 3, a wind vibration assembly 5 is installed on the inner wall of the bottom port of the tank body 1, a cylindrical filter membrane 6 sleeved on the outer periphery of the spiral electric field assembly 3 is connected between the wind vibration assembly 5 and the material atomization assembly 4, a gas cavity 7 is arranged at the top in the tank body 1, a ceramic tube 8 penetrates the middle part of the spiral electric field assembly 3, the ceramic tube 8 vertically rotates through the top of the transonic vortex airflow field generating block 2, and the bottom port of the ceramic tube 8 is connected with a compressor to provide a source gas with high pressure, which can be high-purity nitrogen.

[0038] The motor 201 is fixedly connected to the top of the tank body 1, the output end of the motor 201 vertically and downward penetrates the top of the tank body 1 in a sealing manner, and a plurality of connecting rods 202 arranged in an annular array are fixedly connected to the output end, the bottom of the connecting rod 202 is fixedly connected to the top of the transonic vortex airflow field generating block 2, a plurality of arc-shaped grooves 203 are vertically and downward arranged on the outer wall of the transonic vortex airflow field generating block 2, and the transonic vortex airflow field generating block 2 is a circular block; the motor 201 drives the transonic vortex airflow field generating block 2 to rotate, and the cooling gas is discharged from the bottom port of the arc-shaped groove 203 in a transonic manner, the airflow spirally downward along the inner wall of the tank body 1, a forced vortex is formed, that is, a high-speed, stable and symmetrical vortex airflow field is formed in the tank body 1, and different quality atomized droplets in the vortex field receive a huge centrifugal force, the liquid droplets with large mass, mainly containing un-enzymolyzed macromolecular proteins, gum, mucopolysaccharide and other impurities, receive a huge centrifugal force, are rapidly thrown to the inner wall of the tank body 1, are gathered on the wall surface, flow downward, the target collagen peptides with small mass receive a small centrifugal force, are dragged by the gas viscous force, and are kept near the vortex center area, so that the active separation based on the mass is realized.

[0039] The helical electric field assembly 3 comprises two helical electrode bands 301 fixedly sleeved on the outer wall of the ceramic tube 8, forming double helical electrodes; the outer periphery of the two helical electrode bands 301 is sleeved with a sleeve 302, the ceramic tube 8 is fixedly penetrated at both ends of the sleeve 302, the ceramic tube 8 is an aluminum nitride ceramic tube, the cylindrical filter membrane 6 is sleeved on the outer periphery of the sleeve 302, the outer wall of the sleeve 302 close to the bottom is fixedly connected with a first connecting rod 303, the end of the first connecting rod 303 away from the sleeve 302 is fixedly connected with the inner wall of the tank body 1, the outer wall of the sleeve 302 close to the bottom is sleeved with a discharge port 304, the first connecting rod 303 is fixedly penetrated on the outer wall of the discharge port 304, the inner wall of the discharge port 304 close to the top is fixedly sleeved on the outer wall of the cylindrical filter membrane 6 close to the bottom; the two helical electrode bands 301 have the same pitch and are parallel in space but not in contact; the starting ends of the two helical electrode bands 301 are connected to a double-channel pulse power source outside the cavity through extremely fine platinum wires, when the double-channel power source applies high-voltage pulses with a phase difference of 90° to the two helical electrode bands 301, the voltage transient values on the two bands change high and low, and the voltage difference in space and time composes a point with extremely high electric field strength between the two bands and the surrounding space, which moves along the direction of the helical line over time, thereby forming a rotating forward traveling wave electric field; the traveling wave electric field generates a strong dielectrophoresis traction on the polarized droplets, like a conveyor belt, actively dragging the droplets to the direction of the two helical electrode bands 301 and transporting them downstream of the electrodes, so that the target peptide droplets staying near the vortex center region pass through the cylindrical filter membrane 6, are adsorbed on the outer wall of the sleeve 302, and are transported downward at the same time, and are discharged from the discharge port 304 for collection; the heat generated by the two helical electrode bands 301 can be taken away by the cooling gas in the ceramic tube 8, avoiding the target peptide from being deteriorated by heat; because the cooling gas in the ceramic tube 8 flows continuously, the heat absorbed is not enough to significantly increase the heat of the cooling gas, so the cooling gas entering the tank body 1 also continuously maintains a low temperature, ensuring the quality of the target peptide.

[0040] The material atomization assembly 4 comprises a ring-shaped cavity 401 fixedly sleeved on the top outer wall of the sleeve 302, the ring-shaped cavity 401 is connected with a fish scale enzyme solution supply pump, the bottom of the ring-shaped cavity 401 is fixedly installed with a plurality of ultrasonic atomizing nozzles 402 arranged in a ring array, for atomizing the viscous fish scale enzyme solution into micron-sized droplets through ultrasonic high-frequency vibration; the plurality of ultrasonic atomizing nozzles 402 are located on the outer periphery of the cylindrical filter membrane 6.

[0041] The wind vibration assembly 5 comprises a wind vibration cylinder 501 fixedly sleeved on the outer wall of the cylindrical filter membrane 6, the bottom port of the wind vibration cylinder 501 is arranged close to the top port of the discharge port 304, the outer wall close to the bottom of the wind vibration cylinder 501 is fixedly connected with a plurality of vibration isolators 502 arranged in an annular array, the vibration isolators 502 are fixedly connected with the second connecting rods 503 at the ends away from the wind vibration cylinder 501, the ends of the second connecting rods 503 away from the vibration isolators 502 are fixedly connected with the inner wall of the tank body 1, and the outer wall close to the top of the wind vibration cylinder 501 is fixedly sleeved with a horn-shaped material guide cover 504; the wind vibration assembly 5 further comprises a first annular plate 505 rotatably sleeved on the inner wall of the tank body 1, the inner wall of the first annular plate 505 is fixedly connected with a plurality of impeller blades 506 arranged in an annular array, the plurality of impeller blades 506 are fixedly connected with a second annular plate 507 at the ends away from the first annular plate 505, the second annular plate 507 is located below the bottom port edge of the horn-shaped material guide cover 504, the inner wall of the second annular plate 507 is fixedly connected with a plurality of rectangular fan plates 508 arranged in an annular array, the outer wall of the wind vibration cylinder 501 below the horn-shaped material guide cover 504 is fixedly connected with a plurality of elastic steel sheets 509 arranged in an annular array, and the top of the first annular plate 505 and the second annular plate 507 close to the side of the impeller blade 506 is arranged in an inverted inclined angle; the first annular plate 505, the impeller blade 506 and the second annular plate 507 form an impeller, the vortex airflow passes through the gaps between the plurality of impeller blades 506 downward, and then drives the impeller to rotate, so that the plurality of rectangular fan plates 508 fan the plurality of elastic steel sheets 509, the plurality of elastic steel sheets 509 generate wind vibration, the wind vibration cylinder 501 generates wind vibration, the wind vibration drives the cylindrical filter membrane 6 to vibrate, the impurities adhered to the outer wall of the cylindrical filter membrane 6 are shaken off, and the target peptide is ensured to pass normally; the vibration characteristics can also bounce the target peptide intercepted by the cylindrical filter membrane 6 again, and then pass through the cylindrical filter membrane 6 again, which is like a vibrating screen for screening.

[0042] The gap between the inner wall of the cylindrical filter membrane 6 and the outer wall of the sleeve 302 is 2-5 mm, and the cylindrical filter membrane 6 is in a tension state; the cylindrical filter membrane 6 is used to intercept impurities escaping from the vortex airflow.

[0043] Working principle: when the fish scale collagen peptide extraction and filtration device is used, first, the compressor is connected with the bottom port of the ceramic pipe 8, high-pressure high-purity nitrogen gas is introduced into the ceramic pipe 8 as cooling gas, and the motor 201 is started, the motor 201 drives the transonic vortex airflow field generating block 2 to rotate in the tank body 1 through the connecting rod 202. The cooling gas flows out from the top of the ceramic pipe 8 into the air chamber 7, under the rotating action of the transonic vortex airflow field generating block 2, the cooling gas is discharged from the bottom port of the arc-shaped groove 203 in a transonic posture, and forms a high-speed, stable and symmetrical vortex airflow field along the inner wall of the tank body 1.

[0044] Then, the fish scale enzymatic hydrolysate supply pump is started to deliver the fish scale enzymatic hydrolysate into the annular cavity 401, and the ultrasonic atomizing nozzle 402 at the bottom of the annular cavity 401 atomizes the viscous fish scale enzymatic hydrolysate into micron-sized droplets through ultrasonic high-frequency vibration, and the droplets enter the vortex airflow field in the tank body 1. Under the action of the centrifugal force of the vortex airflow field, the liquid droplets with large mass are subjected to large centrifugal force, and the impurities such as unhydrolyzed macromolecular proteins, gums and mucopolysaccharides are thrown to the inner wall of the tank body 1, and then flow downward after gathering on the wall; the liquid droplets with small mass are subjected to small centrifugal force, and are dragged by the gas viscous force to stay near the vortex center region.

[0045] Subsequently, the double-channel pulse power is connected to the two spiral electrode strips 301 through platinum wires, high-voltage pulses with a phase difference of 90° are applied to the two spiral electrode strips 301, and a rotating forward traveling wave electric field is formed. The traveling wave electric field generates a strong dielectrophoresis traction force on the polarized target peptide droplets, drags them to the direction of the spiral electrode strips 301 and transports them downstream of the electrode, so that the target peptide droplets pass through the cylindrical filter membrane 6 and are adsorbed on the outer wall of the sleeve 302, and then flow downward along the outer wall of the sleeve 302 and are discharged from the discharge port 304 for collection. At the same time, the heat generated by the spiral electrode strips 301 is taken away by the cooling gas flowing in the ceramic tube 8, so as to ensure that the temperature in the tank body 1 is continuously low, and to avoid the target peptide from being deteriorated by heat.

[0046] In the process of the vortex airflow flowing downward, the airflow passes through the gap between the impeller blades 506 to drive the rotation of the impeller composed of the first annular plate 505, the impeller blades 506 and the second annular plate 507, the second annular plate 507 drives the rotation of the rectangular sector plate 508 on the inner wall of the second annular plate 507, the rectangular sector plate 508 fans the elastic steel sheet 509, the elastic steel sheet 509 generates wind vibration, the wind vibration is transmitted to the wind vibration cylinder 501, and the wind vibration cylinder 501 drives the frequency vibration of the cylindrical filter membrane 6. The frequency vibration on the one hand shakes off the impurities adhered to the outer wall of the cylindrical filter membrane 6 to ensure that the target peptide passes through normally, and on the other hand bounces up the target peptide intercepted by the cylindrical filter membrane 6 again to pass through the cylindrical filter membrane 6 again, thereby improving the recovery rate of the target peptide.

[0047] Finally, the impurities thrown to the inner wall of the tank body 1 flow downward along the inner wall of the tank body 1, are guided by the horn-shaped material guide cover 504, are discharged from the opening at the bottom of the tank body 1, and the whole process of extracting and filtering the fish scale collagen peptide is completed.

[0048] The above embodiments only exemplarily illustrate the principles and effects of the present application, and are not used to limit the present application. Any person skilled in the art can modify or change the above embodiments without departing from the spirit and scope of the present application. Therefore, all equivalent modifications or changes completed by those skilled in the art without departing from the spirit and technical thought disclosed by the present application should be covered by the claims of the present application.

Claims

1. A fish scale collagen peptide extraction filter device comprising a tank body (1) with an open bottom, characterized in that, The inner wall of the tank body (1) near the top is rotatably sleeved with a transonic vortex airflow field generating block (2), the tank body (1) is installed with a spiral electric field assembly (3) at the axis below the transonic vortex airflow field generating block (2), the top of the spiral electric field assembly (3) is installed with a material atomization assembly (4), the inner wall of the bottom port of the tank body (1) is installed with a wind vibration assembly (5), the wind vibration assembly (5) and the material atomization assembly (4) are connected with a cylindrical filter membrane (6) sleeved on the outer periphery of the spiral electric field assembly (3), the top of the inside of the tank body (1) is provided with an air cavity (7), the middle of the spiral electric field assembly (3) penetrates a ceramic tube (8), the ceramic tube (8) vertically upwardly penetrates the top of the transonic vortex airflow field generating block (2); the spiral electric field assembly (3) comprises two spiral electrode bands (301) fixedly sleeved on the outer wall of the ceramic tube (8), forming double spiral electrodes, high-voltage pulses with a phase difference of 90° are applied to the two spiral electrode bands (301), forming a rotating forward traveling wave electric field.

2. The fish scale collagen peptide extraction and filtration device according to claim 1, characterized in that: The top of the tank body (1) is fixedly connected with a motor (201), the output end of the motor (201) vertically downwardly penetrates the top of the tank body (1) in a sealing manner, and the output end is fixedly connected with a plurality of connection rods (202) arranged in an annular array, the bottom of the connection rod (202) is fixedly connected with the top of the transonic vortex airflow field generating block (2), a plurality of arc-shaped grooves (203) are vertically and downwardly formed in the outer wall of the transonic vortex airflow field generating block (2), and the transonic vortex airflow field generating block (2) is a circular block.

3. The fish scale collagen peptide extraction and filtration device according to claim 1, characterized in that: The outer periphery of the two spiral electrode bands (301) is sleeved with a sleeve pipe (302), the ceramic tube (8) is fixedly penetrated in both ends of the sleeve pipe (302), the ceramic tube (8) is an aluminum nitride ceramic tube, the cylindrical filter membrane (6) is sleeved on the outer periphery of the sleeve pipe (302), the outer wall of the sleeve pipe (302) near the bottom is fixedly connected with a first connection rod (303), one end of the first connection rod (303) away from the sleeve pipe (302) is fixedly connected with the inner wall of the tank body (1), the outer periphery of the sleeve pipe (302) near the bottom is sleeved with a discharge port (304), the first connection rod (303) is fixedly penetrated in the outer wall of the discharge port (304), and the inner wall of the discharge port (304) near the top is fixedly sleeved with the outer wall of the cylindrical filter membrane (6) near the bottom.

4. The fish scale collagen peptide extraction and filtration device according to claim 3, characterized in that: The material atomization assembly (4) comprises an annular cavity (401) fixedly sleeved on the top outer wall of the sleeve pipe (302), the annular cavity (401) is connected with a fish scale enzyme solution supply pump, the bottom of the annular cavity (401) is fixedly installed with a plurality of ultrasonic atomization nozzles (402) arranged in an annular array, for atomizing the viscous fish scale enzyme solution into micron-sized droplets through ultrasonic high-frequency vibration; a plurality of the ultrasonic atomization nozzles (402) are located on the outer periphery of the cylindrical filter membrane (6).

5. The fish scale collagen peptide extraction and filtration device according to claim 3, characterized in that: The wind vibration assembly (5) comprises a wind vibration cylinder (501) fixedly sleeved on the outer wall of the cylindrical filter membrane (6), the bottom port of the wind vibration cylinder (501) is arranged close to the top port of the discharge port (304), the outer wall close to the bottom of the wind vibration cylinder (501) is fixedly connected with a plurality of vibration isolators (502) arranged in an annular array, the vibration isolator (502) away from the wind vibration cylinder (501) is fixedly connected with a second connecting rod (503), the end of the second connecting rod (503) away from the vibration isolator (502) is fixedly connected with the inner wall of the tank body (1), and the outer wall close to the top of the wind vibration cylinder (501) is fixedly sleeved with a horn-shaped material guide cover (504); the wind vibration assembly (5) further comprises a first annular plate (505) rotatably sleeved on the inner wall of the tank body (1), the inner wall of the first annular plate (505) is fixedly connected with a plurality of impeller blades (506) arranged in an annular array, the ends of the plurality of impeller blades (506) away from the first annular plate (505) are fixedly connected with a second annular plate (507), the second annular plate (507) is located below the bottom port edge of the horn-shaped material guide cover (504), the inner wall of the second annular plate (507) is fixedly connected with a plurality of rectangular fan plates (508) arranged in an annular array, the outer wall of the wind vibration cylinder (501) below the horn-shaped material guide cover (504) is fixedly connected with a plurality of elastic steel sheets (509) arranged in an annular array, and the top of the first annular plate (505) and the second annular plate (507) close to the side of the impeller blade (506) is arranged in an inverted inclined angle.

6. The fish scale collagen peptide extraction and filtration device according to claim 1, characterized in that: The gap between the inner wall of the cylindrical filter membrane (6) and the outer wall of the sleeve (302) is 2-5 mm, and the cylindrical filter membrane (6) is in a tension state.

7. A method for extracting and filtering fish scale collagen peptides, characterized in that: The fish scale collagen peptide extraction and filtration device comprises the following steps: S1, the spiral electric field assembly (3) applies a pulsed electric field, the annular cavity (401) is connected with a fish scale enzymatic hydrolysate supply pump, and the bottom port of the ceramic tube (8) is connected with a cooling gas compressor to provide cooling gas with high pressure; S2, the cooling gas enters the air cavity (7) through the ceramic tube (8), and the transonic vortex flow field generating block (2) rotates at the same time, the high-pressure gas is discharged from the bottom port of the arc-shaped groove (203) in a transonic state under the assistance of the rotation of the transonic vortex flow field generating block (2), and a transonic vortex flow field is formed; S3, the material atomization assembly (4) sprays the viscous fish scale enzymatic hydrolysate downward in a mist state, the liquid drops with large mass are quickly thrown to the inner wall of the tank body (1) and gather on the wall surface to flow downward; The target collagen peptide with small mass is subjected to small centrifugal force and is dragged by the gas viscous force to stay near the vortex center region; S4, the spiral electric field assembly (3) actively drags and transports the liquid drops to the direction of the axis of the tank body (1) and downward, so that the target peptide liquid drops staying near the vortex center region pass through the cylindrical filter membrane (6) and are adsorbed on the outer wall of the sleeve (302) while being transported downward, and are discharged from the discharge port (304) for collection. S5, the heat generated by the helical electric field assembly (3) can be taken away by the cooling gas in the ceramic tube (8), and the cooling gas entering the tank (1) can also continuously maintain low temperature, ensuring the quality of the target peptide; S6, the cylindrical filter membrane (6) intercepts impurities escaping from the vortex gas flow, and the vortex gas flow passes through the wind-shaking assembly (5) with impurities, which drives the cylindrical filter membrane (6) to vibrate, shakes off the impurities adhering to the outer wall of the cylindrical filter membrane (6), and ensures that the target peptide passes normally.

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