Organic water-soluble fertilizer as well as preparation method and application thereof

Abstract

The invention relates to the technical field of agricultural liquid fertilizers, and particularly discloses an organic water-soluble fertilizer as well as a preparation method and application thereof. The organic water-soluble fertilizer disclosed by the invention specifically comprises the following components: 8-14g / L of bacillus velezensis fermentation liquor; 80 to 150g / L of seaweed zymolyte; 80-150 g / L of a seaweed aqueous extract; the solvent is water; the pH (Potential of Hydrogen) value is 4.5 to 6.5; a preparation method of the bacillus velezensis fermentation liquor comprises the step of fermenting a fennel extracting solution and soybean enzymatic hydrolysate according to the weight ratio of (0.1-0.5): (1-10) by utilizing bacillus velezensis to obtain the bacillus velezensis fermentation liquor. By utilizing the technical scheme, the organic water-soluble fertilizer with high content of organic matters, high content of nitrogen, phosphorus and potassium and low content of water-insoluble substances is obtained, and the application prospect of the water-soluble fertilizer is widened.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of agricultural liquid fertilizer, and particularly relates to an organic water-soluble fertilizer and a preparation method and application thereof. BACKGROUND

[0002] In current agricultural production, long-term and large-scale use of chemical fertilizer leads to increasingly serious problems such as soil compaction and acidification, and organic fertilizer is paid attention to due to its ability to improve soil structure and increase soil fertility. As a new type of fertilizer, water-soluble fertilizer has the characteristics of high nutrient content, good solubility and high utilization rate, but traditional organic water-soluble fertilizer has problems such as low organic matter content and unbalanced nutrients.

[0003] The common organic water-soluble fertilizer on the market mainly has the following preparation methods: 1. Animal-derived organic matter (such as fish protein, amino acid, etc.) is prepared by enzymatic process, and this kind of fertilizer has high nitrogen content but lacks phosphorus and potassium; 2. Plant-derived organic matter (such as soybean meal, straw, etc.) is prepared by fermentation process, and this kind of fertilizer has low organic matter content and poor water solubility; 3. Organic-inorganic compound water-soluble fertilizer prepared by chemical synthesis, although this kind of fertilizer has balanced nutrients, it lacks real organic components. In addition, there is also a method of directly dissolving seaweed acid, but the nutrient content does not meet the standard.

[0004] The existing organic water-soluble fertilizer has the following defects: insufficient organic matter content (usually less than 150 g / L), unreasonable macronutrient ratio (N+P2O5+K2O is usually less than 50 g / L), high water-insoluble content (usually more than 80 g / L), and unstable pH value, which seriously affects the fertilizer efficiency and use convenience. SUMMARY

[0005] In order to solve the above technical problems, the present application provides an organic water-soluble fertilizer and a preparation method and application thereof.

[0006] In a first aspect, the present application provides an organic water-soluble fertilizer, which specifically comprises the following components at the following concentrations: Bacillus velezensis fermentation broth 30-40 g / L; seaweed enzymatic hydrolysate 80-150 g / L; seaweed water extract 80-150 g / L; solvent is water; pH value is 4.5-6.5. The preparation method of the Bacillus velezensis fermentation broth is as follows: using Bacillus velezensis to ferment anise extract solution and soybean enzymatic hydrolysate with a weight ratio of 0.1-0.5:1-10 to obtain.

[0007] Seaweed extract is explored as a raw material for organic fertilizer due to its rich active substances, but how to effectively convert it into high-quality water-soluble fertilizer is still a technical problem. In particular, seaweed-derived organic water-soluble fertilizer is difficult to meet the requirements of high organic matter content and reasonable macronutrient ratio at the same time in the prior art.

[0008] The technical solution provided in this application achieves a water-soluble fertilizer with high nutrient efficiency (increasing the bioavailability of organic matter and NPK) and physical stability (low water-insoluble matter content and stable pH) through the triple mechanism of microbial metabolic transformation by Bacillus Velez, decomposition of anti-nutritional factors, generation of active substances, enzymatic bioactivation, release of small molecule nutrients, and seaweed colloid stabilization, chelation, slow release, and buffering.

[0009] Among them, Bacillus velezensis can secrete a variety of enzymes, such as cellulase, protease, and lipase, which break down large organic molecules in fennel extract and soybean hydrolysate, such as polysaccharides, proteins, and lipids, to produce small peptides, amino acids, organic acids, etc., thereby improving nutrient bioavailability. Fennel extract contains volatile oils and phenolic substances, which are converted into more easily absorbed active ingredients after fermentation, with both antibacterial and growth-promoting effects. Soybean hydrolysate is rich in oils, proteins, and polysaccharides, and after fermentation, it produces oligopeptides and oligosaccharides, which provide carbon, nitrogen, and energy sources, enhance bacterial resistance, promote spore formation, and can be further fermented by the strain into organic acids, which lower the pH and chelate minerals, increasing nutrient solubility.

[0010] The seaweed hydrolysate of the present application can break down fucoidan into fucoidan oligosaccharides, release free amino acids and monosaccharides, and improve bioavailability; the seaweed water extract contains active polysaccharides and easily forms a three-dimensional network colloid, which is beneficial to improving element and pH stability.

[0011] Preferably, the organic water-soluble fertilizer specifically comprises the following components in the following concentrations: 32-38 g / L of Bacillus Velez fermentation broth; 80-100 g / L of seaweed hydrolysate; 130-150 g / L of seaweed water extract; and the solvent is water.

[0012] In a specific embodiment, the organic water-soluble fertilizer specifically includes the following components in concentrations: 35 g / L of Bacillus Velez fermentation broth; 90 g / L of seaweed hydrolysate; 140 g / L of seaweed water extract; and the solvent is water.

[0013] Preferably, the preparation method of the Bacillus Velez fermentation broth is: using Bacillus Velez to ferment fennel extract and soybean enzymatic hydrolyzate in a weight ratio of 0.2-0.4:4-7 to obtain the broth.

[0014] Preferably, in the method for preparing the Bacillus Velez fermentation broth: The preparation method of the fennel extract is as follows: crushing the dried fennel seeds, and sealingly extracting them in hot water or ethanol at 55-70° C. for 3-5 hours. After filtration and concentration to a density of 1.05-1.3g / cm 3 ; The preparation method of the soybean enzymatic solution is as follows: after soybean is crushed, it is placed in 0.01-0.03wt% alkaline protease aqueous solution, hydrolyzed at 40-60℃ and pH=7.5-8.5 for 6-8h, filtered after enzyme inactivation, concentrated to a density of 1.05-1.3g / cm 3 .

[0015] Preferably, the preparation method of the B. velezensis fermentation liquor is as follows: corresponding weight ratios of anise extract solution and soybean enzymatic solution are mixed, 0.8-1.2 times of water is added to obtain a fermentation material; 0.003-0.009 parts of B. velezensis with a bacterial content of 1-9×10 8 CFU / ml is uniformly dispersed in 1-1.4 parts of the fermentation material, fermentation is carried out at 30-50℃ for 24-48h, a filtrate is obtained by filtration, and the B. velezensis fermentation liquor is obtained by sterilization.

[0016] Preferably, the preparation method of the B. velezensis fermentation liquor is as follows: corresponding weight ratios of anise extract solution and soybean enzymatic solution are mixed, 0.8-1.2 times of water is added to obtain a fermentation material; 0.003-0.009 parts of B. velezensis with a bacterial content of 1-9×10 8 CFU / ml is uniformly dispersed in 1-1.4 parts of the fermentation material, fermentation is carried out at 30-50℃ for 24-48h, a filtrate is obtained by filtration, and the B. velezensis fermentation liquor is obtained by sterilization.

[0017] Preferably, the preparation method of the seaweed water extract is as follows: seaweed powder is soaked in 8-12 times of water, decocted for 100-180min, a filtrate is obtained by filtration, and dried to obtain the seaweed water extract.

[0018] Preferably, the preparation method of the seaweed enzymatic solution is as follows: seaweed powder is soaked in 8-12 times of enzymatic solution, extracted at 30-40℃ for 120-180min; a filtrate is obtained by filtration, and dried to obtain the seaweed enzymatic solution; the enzymatic solution is composed of cellulase, pectinase, papain and water at a weight ratio of 0.005-0.015:0.01-0.05:0.07-0.11:1000.

[0019] Preferably, in the preparation method of the seaweed enzymatic solution, the weight ratio of cellulase, pectinase, papain and water is 0.007-0.012:0.02-0.04:0.08-0.10:1000.

[0020] In a specific embodiment, in the preparation method of the seaweed enzymatic solution, the weight ratio of cellulase, pectinase, papain and water is 0.01:0.03:0.09:1000.

[0021] Preferably, the performance parameters of the organic water-soluble fertilizer are: organic matter content ≥ 150 g / L; total amount of N+P2O5+K2O ≥ 60 g / L; water-insoluble substance ≤ 50 g / L; pH value is 4.5-6.5.

[0022] In a second aspect, the application provides a preparation method of the above-mentioned organic water-soluble fertilizer, which specifically comprises the following steps in sequence: weighing respective amounts of each raw material component, then adding the seaweed water extract, seaweed enzymatic hydrolysate and bacillus velezensis fermentation liquor into water respectively; and adjusting the pH value to 4.5-6.5.

[0023] In summary, the technical solution of the application has the following effects: The embodiments of the application utilize bacillus velezensis to ferment fennel extract and soybean enzymatic hydrolysate to obtain bacillus velezensis fermentation liquor, which is uniformly dispersed in water according to a specific concentration together with seaweed water extract and seaweed enzymatic hydrolysate, thereby obtaining an organic water-soluble fertilizer with high organic matter content, high nitrogen, phosphorus and potassium content, low water-insoluble substance content and stable pH value.

[0024] The water-soluble organic fertilizer prepared by the application has an organic matter content of more than 150 g / L, which is significantly higher than that of conventional organic water-soluble fertilizer; the total amount of N+P2O5+K2O is ≥ 60 g / L, achieving balanced nutrient supply; the water-insoluble substance content is ≤ 50 g / L, with excellent dissolution performance; the pH value is stable within the range of 4.5-6.5, with wide applicability; the seaweed-derived active substances are retained intact, with additional functions such as growth promotion and stress resistance. DETAILED DESCRIPTION

[0025] The application will be further described in detail below in combination with examples, comparative examples and performance test experiments, and these examples should not be understood as limiting the scope of the application. Examples

[0026] Examples 1-5 Examples 1-5 respectively provide an organic water-soluble fertilizer and a preparation method thereof.

[0027] The differences between the above examples are that the concentrations of the components in the organic water-soluble fertilizer are different, as shown in Table 1.

[0028] The preparation method of the organic water-soluble fertilizer in the above examples is as follows: (1) The preparation of Bacillus velezensis: After 3 times of activation, the test strain (Bacillus velezensis BGB-89R, for details, see the patent application filed by the applicant on December 29, 2023, with the application number CN202311856789.6) stored at -80℃ was transferred to LB culture medium for culture at 30℃ and 180 rpm until the logarithmic phase. The growth of the strain was detected by a nucleic acid protein detector, the bacterial content was estimated by OD value, and the sporulation rate was calculated by dilution and plating. When the sporulation rate reached more than 90%, the culture was centrifuged and concentrated, and then diluted with sterile water to a bacterial content of 5×10 8 CFU / ml. The sample was placed in a 6℃ refrigerator for later use.

[0029] (2) The preparation method of Bacillus velezensis fermentation broth is as follows: The preparation method of anise extract is as follows: dry anise seeds are ground, immersed in hot water at 60℃ for 4h, filtered, and concentrated to a density of 1.15g / cm 3 .

[0030] The preparation method of soybean enzymatic hydrolysate is as follows: soybean is ground and placed in 0.02wt% alkaline protease aqueous solution, hydrolyzed at pH=8.0 and 50℃ for 7h, filtered after enzyme inactivation, and concentrated to a density of 1.15g / cm 3 .

[0031] Anise extract and soybean enzymatic hydrolysate with a weight ratio of 0.3kg:5.5kg are mixed uniformly, 1 times the amount of water is added, and 0.006kg of Bacillus velezensis prepared above is uniformly dispersed in 1.2kg of the mixture to be fermented. Fermentation is carried out at 40℃ for 36h, the filtrate is obtained by filtration, and the Bacillus velezensis fermentation broth is obtained by sterilization at 121℃.

[0032] (3) The preparation method of seaweed water extract is as follows: seaweed powder (particle size ≤100 mesh) is soaked in 10 times the amount of water, decocted for 150min, filtered, and dried to obtain the extract.

[0033] (4) The preparation method of seaweed enzymatic hydrolysate is as follows: seaweed powder (particle size ≤100 mesh) is soaked in 10 times the amount of enzyme solution, extracted at 35℃ for 150min, filtered, and dried to obtain the hydrolysate. The enzyme solution is composed of cellulase, pectinase, papain, and water with a weight ratio of 0.01:0.03:0.09:1000.

[0034] (5) The corresponding weights of each raw material component are weighed, and then the seaweed water extract, seaweed enzymatic hydrolysate, and Bacillus velezensis fermentation broth are added to water respectively. The pH value is adjusted to 5.5.

[0035] Table 1 Concentrations of each component in Examples 1-5 and Comparative Examples 1-2 Examples 6-9 Examples 6-9 respectively provide an organic water-soluble fertilizer and a preparation method thereof.

[0036] The difference between the above examples and Example 3 is specifically as follows: the preparation method of the Bacillus velezensis fermentation broth is different, and is specifically as follows.

[0037] In Example 6: Bacillus velezensis with a bacterial content of 5 x 10 8 CFU / ml is used to ferment anise extract solution and soybean enzymatic hydrolysate with a weight ratio of 0.1:10.

[0038] In Example 7: Bacillus velezensis with a bacterial content of 5 x 10 8 CFU / ml is used to ferment anise extract solution and soybean enzymatic hydrolysate with a weight ratio of 0.5:1.

[0039] In Example 8: Bacillus velezensis with a bacterial content of 5 x 10 8 CFU / ml is used to ferment anise extract solution and soybean enzymatic hydrolysate with a weight ratio of 0.2:7.

[0040] In Example 9: Bacillus velezensis with a bacterial content of 5 x 10 8 CFU / ml is used to ferment anise extract solution and soybean enzymatic hydrolysate with a weight ratio of 0.4:4.

[0041] In the above examples, other process parameters are the same as those in Example 3.

[0042] Examples 10-15 Examples 10-15 respectively provide an organic water-soluble fertilizer and a preparation method thereof.

[0043] The difference between the above examples and Example 3 is specifically as follows: the preparation method of the seaweed enzymatic hydrolysate is different, and is specifically as follows.

[0044] In Example 10: In the preparation method of the seaweed enzymatic hydrolysate: the enzymatic hydrolysate is composed of cellulase, pectinase, papain, and water with a weight ratio of 0.005:0.05:0.07:1000.

[0045] In Example 11: In the preparation method of the seaweed enzymatic hydrolysate: the enzymatic hydrolysate is composed of cellulase, pectinase, papain, and water with a weight ratio of 0.015:0.01:0.11:1000.

[0046] In Example 12: the preparation method of the seaweed enzymatic hydrolysate is as follows: the enzymatic hydrolysate is composed of cellulase, pectinase, papain and water in a weight ratio of 0.007:0.04:0.07:1000.

[0047] In Example 13: the preparation method of the seaweed enzymatic hydrolysate is as follows: the enzymatic hydrolysate is composed of cellulase, pectinase, papain and water in a weight ratio of 0.10:0.03:0.01:1000.

[0048] In Example 14: the preparation method of the seaweed enzymatic hydrolysate is as follows: the enzymatic hydrolysate is composed of cellulase, pectinase, xylanase and water in a weight ratio of 0.01:0.03:0.09:1000.

[0049] In Example 15: the preparation method of the seaweed enzymatic hydrolysate is as follows: the enzymatic hydrolysate is composed of cellulase, pectinase, papain, xylanase and water in a weight ratio of 0.01:0.03:0.06:0.03:1000.

[0050] In the above examples, other process parameters are the same as those in Example 3.

[0051] Comparative Example Comparative Example 1-2 Comparative Example 1-2 respectively provides an organic water-soluble fertilizer and a preparation method thereof.

[0052] The above comparative examples differ from Example 3 in that the concentrations of the components in the organic water-soluble fertilizer are different, as shown in Table 1.

[0053] In the above comparative examples, other process parameters are the same as those in Example 3.

[0054] Comparative Example 3-5 Comparative Example 3-5 respectively provides an organic water-soluble fertilizer and a preparation method thereof.

[0055] The above comparative examples differ from Example 3 in that the concentrations of the components in the organic water-soluble fertilizer are different, as shown in Table 1.

[0056] In Comparative Example 3: an equal amount of Bacillus licheniformis fermentation broth is used to replace the Bacillus velezensis fermentation broth; the preparation method of the Bacillus licheniformis fermentation broth is as follows: 0.3 kg of fennel extract and 5.5 kg of soybean enzymatic hydrolysate are mixed uniformly, 1 times the amount of water is added, and a fermentation material is obtained; 0.006 kg of Bacillus licheniformis with a content of 5×10 8Bacillus licheniformis (Bacillus licheniformis is BGB-85F, for details, see the patent application filed by the applicant on May 5, 2022, application number CN202210483248.2) with a concentration of 5×10

[0057] In Comparative Example 4, the preparation method of the Bacillus velezensis fermentation broth was as follows: Bacillus velezensis with a concentration of 5×10 8 The Bacillus velezensis with a concentration of 5×10

[0058] In Comparative Example 5, the preparation method of the Bacillus velezensis fermentation broth was as follows: Bacillus velezensis with a concentration of 5×10 8 The Bacillus velezensis with a concentration of 5×10

[0059] In the above comparative examples, other process parameters were the same as those in Example 3.

[0060] Performance test Performance of active ingredients: The organic matter, N, P2O5, K2O and seed germination index in the organic water-soluble fertilizer were detected according to the detection method specified in NY / T525-2021 Organic Fertilizer; The detection results are shown in Table 2.

[0061] Table 2: Performance test results of active ingredients in organic water-soluble fertilizers in examples and comparative examples According to Table 4, by comparing the detection results of the examples and the comparative examples, it can be seen that in Comparative Example 1, the water-soluble fertilizer did not add Bacillus velezensis fermentation broth, but an equal amount of seaweed enzyme hydrolysate and seaweed water extract instead of Bacillus velezensis fermentation broth. In Comparative Example 2, the concentration of seaweed enzyme hydrolysate and seaweed water extract was not matched. In Comparative Example 3, an equal amount of Bacillus licheniformis fermentation broth was used instead of Bacillus velezensis fermentation broth, and the comprehensive performance of the prepared organic water-soluble fertilizer was poor. In Comparative Example 4, the preparation method of the Bacillus velezensis fermentation broth was as follows: Bacillus velezensis with a concentration of 5×10

[0062] In contrast, the embodiments of the present application utilize B. velezensis to ferment anise extract solution and soybean enzymatic hydrolysate in a weight ratio of 0.1-0.5:1-10 to obtain B. velezensis fermentation, and seaweed water extract and seaweed enzymatic hydrolysate are uniformly dispersed in water according to a specific concentration to obtain an organic water-soluble fertilizer with an organic matter content of ≥150 g / L, a total amount of N+P2O5+K2O of ≥60 g / L, water-insoluble matter of ≤50 g / L, a pH value of 4.5-6.5, and a seed germination index of greater than 75%.

[0063] Although the present application has been described in detail with general description and specific embodiments above, some modifications or improvements can be made on the basis of the present application, which is obvious to those skilled in the art. Therefore, these modifications or improvements made on the basis of not deviating from the spirit of the present application, all belong to the scope of protection claimed by the present application.

Claims

1. An organic water-soluble fertilizer, characterized by, Specifically comprising the following concentrations of components: Bacillus velezensis fermentation broth 30-40 g / L; seaweed enzymatic hydrolysate 80-150 g / L; seaweed water extract 80-150 g / L; solvent is water; pH value is 4.5-6.

5. The preparation method of the Bacillus velezensis fermentation broth is as follows: using Bacillus velezensis to ferment anise extract liquid and soybean enzymatic hydrolysate in a weight ratio of 0.1-0.5:1-10 to obtain.

2. The organic water soluble fertilizer according to claim 1, characterized in that, Specifically comprising the following concentrations of components: Bacillus velezensis fermentation broth 32-38 g / L; seaweed enzymatic hydrolysate 80-100 g / L; seaweed water extract 130-150 g / L; solvent is water.

3. The organic water soluble fertilizer according to claim 1, characterized in that, The preparation method of the Bacillus velezensis fermentation broth is as follows: using Bacillus velezensis to ferment anise extract liquid and soybean enzymatic hydrolysate in a weight ratio of 0.2-0.4:4-7 to obtain.

4. The organic water soluble fertilizer according to claim 1, characterized in that, The preparation method of the Bacillus velezensis fermentation broth is as follows: The preparation method of the fennel extract is as follows: grinding dried fennel seeds, closed extraction in 55-70 DEG C hot water or ethanol for 3-5 hours, filtering, concentrating to the density of 1.05-1.3 g / cm 3 ; The preparation method of the soybean enzymatic hydrolysate is as follows: soybean is crushed, and then placed in 0.01-0.03wt% alkaline protease aqueous solution, and hydrolyzed at pH=7.5-8.5 and 40-60℃ for 6-8h. After enzyme inactivation, filtration is performed, and concentration is performed to a density of 1.05-1.3g / cm 3 .

5. The organic water soluble fertilizer according to claim 4, characterized in that, The preparation method of the Bacillus velezensis fermentation liquor is as follows: taking aniline extraction liquid and soybean enzymolysis liquid in a corresponding weight ratio, adding 0.8-1.2 times of water to obtain a fermentation material; taking 0.003-0.009 parts of Bacillus velezensis with a bacterial content of 1-9×10 8 CFU / ml in a weight part, uniformly dispersing in 1-1.4 parts of the fermentation material, and carrying out fermentation at 30-50°C for 24-48h to obtain a filtrate, and the Bacillus velezensis fermentation liquor is obtained after sterilization.

6. The organic water soluble fertilizer according to claim 1, characterized in that, The preparation method of the seaweed water extract is as follows: soaking seaweed powder in 8-12 times the amount of water, decocting for 100-180 min, filtering to obtain the filtrate, and drying to obtain the seaweed water extract.

7. The organic water soluble fertilizer according to claim 1, characterized in that, The preparation method of the seaweed enzymatic hydrolysate is as follows: soaking seaweed powder in 8-12 times the amount of enzymatic hydrolysate, extracting at 30-40°C for 120-180 min; filtering to obtain the filtrate, and drying to obtain the seaweed enzymatic hydrolysate; the enzymatic hydrolysate is composed of cellulase, pectinase, papain, and water in a weight ratio of 0.005-0.015:0.01-0.05:0.07-0.11:1000.

8. The organic water soluble fertilizer according to claim 7, characterized in that, In the preparation method of the seaweed enzymatic hydrolysate, the weight ratio of cellulase, pectinase, papain, and water is 0.007-0.012:0.02-0.04:0.08-0.10:1000.

9. The organic water soluble fertilizer according to claim 1, characterized in that, The performance parameters of the organic water-soluble fertilizer are as follows: organic matter content ≥150 g / L; total amount of N+P2O5+K2O ≥60 g / L; water-insoluble substance ≤50 g / L; pH value is 4.5-6.

5.

10. The method for preparing an organic water-soluble fertilizer according to any one of claims 1 to 9, wherein Specifically comprising the following steps: weighing the respective weights of each raw material component, then adding seaweed water extract, seaweed enzymatic hydrolysate, and Bacillus velezensis fermentation broth into water, respectively; and adjusting the pH value to 4.5-6.5.

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