Microbial agent capable of degrading long-chain fats in kitchen waste and preparation method thereof

Abstract

The application discloses a kind of degradable kitchen garbage long-chain fat microbial inoculant and preparation method thereof, belong to kitchen garbage processing technical field, specifically related to the preparation of liquid bacterial solution by mixing bacillus subtilis liquid, bacillus licheniformis liquid and Candida lipolytica liquid, then mixed with conditioner including coconut husk, rice husk and biological activated carbon, to prepare microbial inoculant, in the preparation of biological activated carbon, modified solution containing sodium alginate, calcium nitrate and peanut shell are mixed, after calcination carbonization, to prepare biological activated carbon;In the preparation of biological activated carbon, amino compound can also be added, amino compound includes 2-aminothiazole hydrochloride and amino-(2,4-dihydroxy-phenyl)-acetic acid.The application provides a kind of microbial inoculant with good microbial survival rate, long storage time and good oil degradation effect and preparation method thereof.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of kitchen waste treatment, and particularly relates to a microbial agent capable of degrading long-chain fat of kitchen waste and a preparation method thereof. BACKGROUND

[0002] Kitchen waste, also known as organic waste or perishable waste, refers to perishable biomass household waste such as food material waste, leftover food, expired food, melon rind, fruit pits, flowers and green plants, and has the characteristics of easy rotting, low heat value, and rich organic matter content. Conventional landfill and incineration are difficult to properly handle, are more likely to cause environmental pollution, and have certain safety hazards. At present, kitchen waste is mainly treated by using biological degradation technology, that is, according to the characteristics of the market breeding of some microorganisms with degradation function, using professional biological fermentation equipment, high-speed fermentation at a suitable temperature, converting kitchen waste into relatively stable humus, removing carbon dioxide and water vapor, and finally converting into organic fertilizer. In the microbial treatment of kitchen waste, the aerobic composting technology has gradually become the main way of kitchen waste resource regeneration. The types of microorganisms and enzyme activity are the main factors affecting the composting time and the quality of organic fertilizer, and mainly have a degradation effect on protein, oil, starch and cellulose. However, the existing technology still has the problem of poor degradation effect. Therefore, developing microorganisms that can efficiently degrade the main components of kitchen waste and determining the optimal degradation conditions can better serve agriculture, urban pollution control and other aspects, and maximize resource utilization. SUMMARY

[0003] The application aims to provide a microbial agent capable of degrading oil in kitchen waste, which has good survival rate of microorganisms, long storage time and good oil degradation effect, and a preparation method thereof.

[0004] The technical scheme adopted by the application to achieve the above-mentioned purpose is as follows:

[0005] A microbial agent comprises: liquid bacterial solution and conditioning agent, the use amount of the liquid bacterial solution is 50-100wt% of the conditioning agent; the conditioning agent comprises bioactive carbon, and the bioactive carbon has a carbon compound of alginate and calcium. The liquid bacterial solution and the conditioning agent are mixed, the conditioning agent is a substance with pores, high specific surface area and adsorption capacity, and the liquid bacterial solution is dispersed in the conditioning agent, so that the structure of the obtained microbial agent can be improved, and the degradation of organic matter in kitchen waste can be improved.

[0006] Preferably, the bioactive carbon further has a carbon compound of an amino compound; or, the bioactive carbon further has a carbon compound of peanut shells.

[0007] Preferably, the conditioning agent further comprises coconut husk and / or rice husk.

[0008] More preferably, the use amount of rice husk is 40-60wt% of the coconut shell; or, the use amount of bioactive carbon is 80-120wt% of the coconut shell.

[0009] Preferably, the liquid bacterial solution contains at least one of bacillus subtilis, bacillus licheniformis and candida oleophila.

[0010] The application discloses a preparation method of a microbial agent, comprising: mixing a liquid bacterial solution with a conditioning agent to obtain the microbial agent; the use amount of the liquid bacterial solution is 50-100wt% of the conditioning agent; the conditioning agent comprises bioactive carbon, and the bioactive carbon is prepared by mixing a modified solution, calcium nitrate and peanut shells and then carbonizing the mixture; the modified solution contains sodium alginate, and the content of the sodium alginate in the modified solution is 5-20wt%.

[0011] Preferably, the solvent of the modified solution is an ethanol aqueous solution, and the content of ethanol in the ethanol aqueous solution is 30-70wt%.

[0012] Preferably, the liquid bacterial solution is obtained by mixing bacillus subtilis liquid, bacillus licheniformis liquid and candida oleophila liquid, and the bacillus subtilis liquid, the bacillus licheniformis liquid and the candida oleophila liquid are mixed in a volume ratio of 1:0.1-10:0.1-10.

[0013] Preferably, the modified solution further contains an amino compound, and the amino compound comprises 2-aminothiazole hydrochloride and / or amino-(2,4-dihydroxy-phenyl)-acetic acid. In the application, the peanut shells, the sodium alginate, the calcium nitrate, the 2-aminothiazole hydrochloride and / or the amino-(2,4-dihydroxy-phenyl)-acetic acid are used to prepare the bioactive carbon, the calcium in the sodium alginate and the calcium nitrate forms a cross-linking structure, and then the 2-aminothiazole hydrochloride and / or the amino-(2,4-dihydroxy-phenyl)-acetic acid is used to form the bioactive carbon containing calcium alginate, 2-aminothiazole hydrochloride and / or amino-(2,4-dihydroxy-phenyl)-acetic acid, and the bioactive carbon can improve the storage performance of the prepared microbial agent, improve the survival rate of the microbial agent after storage, and improve the degradation rate of oil in kitchen garbage when the microbial agent is applied to the kitchen garbage treatment.

[0014] More preferably, the use amount of the 2-aminothiazole hydrochloride is 10-30wt% of the sodium alginate, and the use amount of the amino-(2,4-dihydroxy-phenyl)-acetic acid is 4-24wt% of the sodium alginate.

[0015] Preferably, the preparation of the microbial agent comprises the preparation of the modified solution.

[0016] More preferably, in the preparation of the modified solution, the sodium alginate is added into the ethanol aqueous solution to obtain the modified solution.

[0017] More preferably, in the preparation of the modified solution, the content of ethanol in the aqueous ethanol solution is 30-70wt%.

[0018] More preferably, in the preparation of the modified solution, the alginate is sodium alginate, and the content of sodium alginate in the modified solution is 5-20wt%.

[0019] More preferably, in the preparation of the modified solution, an amino compound is added. The amino compound is 2-aminothiazole hydrochloride and amino-(2,4-dihydroxy-phenyl)-acetic acid.

[0020] More preferably, in the preparation of the modified solution, the use amount of 2-aminothiazole hydrochloride is 10-30wt% of the sodium alginate, and the use amount of amino-(2,4-dihydroxy-phenyl)-acetic acid is 4-24wt% of the sodium alginate.

[0021] Preferably, the preparation of the microbial agent includes the preparation of the bioactive carbon.

[0022] More preferably, in the preparation of the bioactive carbon, the peanut shell is cleaned, crushed and sieved to obtain peanut shell powder, then the modified solution and calcium nitrate are added, and the mixture is placed at 20-40℃ for 6-24h, and then dried and calcined to obtain the bioactive carbon.

[0023] More preferably, in the preparation of the bioactive carbon, the sieving is through a 40-60 mesh sieve.

[0024] More preferably, in the preparation of the bioactive carbon, the use amount of the modified solution is measured by the amount of alginate therein, and the use amount of the alginate is 10-40wt% of the peanut shell powder.

[0025] More preferably, in the preparation of the bioactive carbon, the use amount of calcium nitrate is 5-20wt% of the peanut shell powder.

[0026] More preferably, in the bioactive carbon, calcium cyclamate can also be added to the modified solution, and the use amount of calcium cyclamate is 1-10wt% of the sodium alginate. After the use of 2-aminothiazole hydrochloride and amino-(2,4-dihydroxy-phenyl)-acetic acid in the preparation of the bioactive carbon, calcium cyclamate can be further added, and the bioactive carbon can be obtained through further carbonization under the mixing action of calcium alginate. Under the mixing action of coconut coir and / or rice husk, the storage performance of the prepared microbial agent and the survival rate of the microbial agent after storage can be further improved, and after being applied to kitchen waste treatment, the degradation rate of oil in kitchen waste can also be improved.

[0027] Preferably, the preparation of the microbial agent includes a liquid culture medium.

[0028] More preferably, in the liquid culture medium, the protein peptone is 5-20 g / L, the yeast extract is 2-10 g / L, the sodium chloride is 5-20 g / L, the magnesium chloride is 0.05-0.5 g / L, the ferrous sulfate heptahydrate is 0.005-0.05 g / L, and the solvent is water, and the pH is adjusted to neutral.

[0029] Preferably, the preparation of the microbial agent includes preparation of the strain expansion liquid.

[0030] More preferably, in the preparation of the strain expansion liquid, the kitchen waste degrading bacteria is inoculated into the liquid culture medium, and the inoculation amount is 1-10 wt% of the liquid culture medium, and the inoculated liquid culture medium is cultured at 20-40℃ for 12-24h to obtain the seed liquid, and then the seed liquid is inoculated into the same liquid culture medium in the same inoculation amount to obtain the strain expansion liquid.

[0031] More preferably, in the preparation of the strain expansion liquid, the inoculation amount of the kitchen waste degrading bacteria is 1-10 wt% of the liquid culture medium.

[0032] More preferably, in the preparation of the strain expansion liquid, the kitchen waste degrading bacteria includes Bacillus subtilis, Bacillus licheniformis, and Candida lipolytica.

[0033] Preferably, the preparation of the microbial agent includes preparation of the liquid bacterial liquid.

[0034] More preferably, in the preparation of the liquid bacterial liquid, the strain expansion liquid is mixed to obtain the liquid bacterial liquid.

[0035] More preferably, in the preparation of the liquid bacterial liquid, the strain expansion liquid includes Bacillus subtilis expansion liquid, Bacillus licheniformis expansion liquid, and Candida lipolytica expansion liquid, and the Bacillus subtilis expansion liquid, the Bacillus licheniformis expansion liquid, and the Candida lipolytica expansion liquid are mixed in a volume ratio of 1:0.1-10:0.1-10.

[0036] Preferably, in the preparation of the microbial agent, the liquid bacterial liquid is mixed with the conditioning agent to obtain the microbial agent.

[0037] More preferably, in the preparation of the microbial agent, the conditioning agent includes coconut husk, rice husk, and bioactive carbon.

[0038] More preferably, in the preparation of the microbial agent, the use amount of the rice husk in the conditioning agent is 40-60 wt% of the coconut husk.

[0039] More preferably, in the preparation of the microbial agent, the use amount of the bioactive carbon in the conditioning agent is 80-120 wt% of the coconut husk.

[0040] More preferably, in the preparation of the microbial agent, the use amount of the liquid bacterial liquid is 50-100 wt% of the conditioning agent.

[0041] This invention discloses the use of the above-mentioned microbial agent in the degradation of kitchen waste.

[0042] This invention utilizes a method of preparing a liquid bacterial solution by mixing Bacillus subtilis, Bacillus licheniformis, and Candida lipolytica, and then mixing this solution with a conditioner containing coconut coir, rice husks, and bioactive carbon to prepare a microbial agent. In the preparation of the bioactive carbon, a modified solution containing sodium alginate, calcium nitrate, and peanut shells are mixed and then calcined and carbonized to obtain the bioactive carbon. Furthermore, amino compounds, including 2-aminothiazole hydrochloride and amino-(2,4-dihydroxyphenyl)-acetic acid, can be added during the preparation of the bioactive carbon. Therefore, this invention provides the following beneficial effects: high microbial survival rate, good storage performance of the prepared microbial agent, and good grease degradation rate when applied to kitchen waste treatment. Thus, this invention is a microbial agent and its preparation method for degrading kitchen waste grease, characterized by high microbial survival rate, long storage time, and good grease degradation effect. Attached Figure Description

[0043] Figure 1 This is an electron micrograph of bioactive carbon.

[0044] Figure 2 This is a graph showing the effective viable bacterial survival rate.

[0045] Figure 3 This is a graph showing the rate of oil degradation. Detailed Implementation

[0046] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0047] The concepts involved in this application will first be described with reference to the accompanying drawings. It should be noted that the following descriptions of various concepts are only for the purpose of making the content of this application easier to understand and do not constitute a limitation on the scope of protection of this application; furthermore, the embodiments and features in the embodiments of this application can be combined with each other unless otherwise specified. This application will now be described in detail with reference to the accompanying drawings and embodiments.

[0048] Example 1: A method for preparing a microbial inoculant

[0049] Preparation of modified solution: the alginate was added into the ethanol aqueous solution to obtain the modified solution. The content of ethanol in the ethanol aqueous solution was 40wt%, the alginate was sodium alginate, and the content of sodium alginate in the modified solution was 10wt%.

[0050] Preparation of bioactive carbon: the peanut shell was cleaned, crushed and sieved to obtain peanut shell powder, then the modified solution and calcium nitrate were added, and the mixture was placed at 30℃ for 12h, and then dried and calcined to obtain bioactive carbon. The sieving was through a 50 mesh sieve. The use amount of the modified solution was measured by the amount of alginate therein, the use amount of alginate was 30wt% of the peanut shell powder, and the use amount of calcium nitrate was 10wt% of the peanut shell powder.

[0051] Liquid medium: 10g / L of proteose peptone, 5g / L of yeast extract, 10g / L of sodium chloride, 0.2g / L of magnesium chloride, 0.01g / L of ferrous sulfate heptahydrate, solvent is water, and pH is adjusted to neutral.

[0052] Preparation of strain expansion liquid: the kitchen waste degrading bacteria was inoculated into the liquid medium and cultured at 37℃ for 16h to obtain the seed liquid, then the same inoculation amount was inoculated into the same new liquid medium to expand the culture, and cultured at 37℃ for 16h to obtain the strain expansion liquid. The inoculation amount of the kitchen waste degrading bacteria was 10wt% of the liquid medium. The kitchen waste degrading bacteria included Bacillus subtilis, Bacillus licheniformis and Candida lipolytica. The Bacillus subtilis had a preservation number of GDMCC1.5711. The Bacillus licheniformis had a preservation number of GDMCC 1.5713. The Candida lipolytica had a preservation number of GDMCC 2.3.

[0053] Preparation of liquid bacterial solution: the strain expansion liquid was mixed to obtain the liquid bacterial solution. The strain expansion liquid included Bacillus subtilis expansion liquid, Bacillus licheniformis expansion liquid and Candida lipolytica expansion liquid, which were mixed in a volume ratio of 1:1:1.

[0054] Preparation of microbial inoculant: the liquid bacterial solution was mixed with the conditioner to obtain the microbial inoculant. The conditioner included coconut husk, rice husk and bioactive carbon, the use amount of rice husk in the conditioner was 50wt% of the coconut husk, the use amount of bioactive carbon in the conditioner was 100wt% of the coconut husk, and the use amount of the liquid bacterial solution was 100wt% of the conditioner.

[0055] Example 2: a preparation method of a microbial inoculant

[0056] Compared with example 1, the difference of this example is the preparation of the modified solution.

[0057] Preparation of the modified solution: the alginate and the amino compound are mixed in the aqueous ethanol solution to obtain the modified solution. The content of ethanol in the aqueous ethanol solution is 40wt%, the alginate is sodium alginate, and the content of sodium alginate in the modified solution is 10wt%; the amino compound is 2-aminothiazole hydrochloride and amino-(2,4-dihydroxy-phenyl)-acetic acid, the usage amount of 2-aminothiazole hydrochloride is 20wt% of sodium alginate, and the usage amount of amino-(2,4-dihydroxy-phenyl)-acetic acid is 12wt% of sodium alginate.

[0058] Example 3: a preparation method of a microbial agent

[0059] The difference between this example and example 2 is the preparation of the modified solution.

[0060] Preparation of the modified solution: the alginate and the amino compound are mixed in the aqueous ethanol solution to obtain the modified solution. The content of ethanol in the aqueous ethanol solution is 40wt%, the alginate is sodium alginate, and the content of sodium alginate in the modified solution is 10wt%; the amino compound is 2-aminothiazole hydrochloride and amino-(2,4-dihydroxy-phenyl)-acetic acid, the usage amount of 2-aminothiazole hydrochloride is 15wt% of sodium alginate, and the usage amount of amino-(2,4-dihydroxy-phenyl)-acetic acid is 10wt% of sodium alginate.

[0061] Example 4: a preparation method of a microbial agent

[0062] The difference between this example and example 2 is the preparation of the modified solution.

[0063] Preparation of the modified solution: the alginate and the amino compound are mixed in the aqueous ethanol solution to obtain the modified solution. The content of ethanol in the aqueous ethanol solution is 40wt%, the alginate is sodium alginate, and the content of sodium alginate in the modified solution is 10wt%; the amino compound is 2-aminothiazole hydrochloride, amino-(2,4-dihydroxy-phenyl)-acetic acid and calcium cyclamate, the usage amount of 2-aminothiazole hydrochloride is 20wt% of sodium alginate, the usage amount of amino-(2,4-dihydroxy-phenyl)-acetic acid is 12wt% of sodium alginate, and the usage amount of calcium cyclamate is 7wt% of sodium alginate.

[0064] Example 5: a preparation method of a microbial agent

[0065] The difference between this example and example 2 is the preparation of the modified solution.

[0066] Preparation of the modified solution: the alginate and the amino compound were mixed in the ethanol aqueous solution to obtain the modified solution. The content of ethanol in the ethanol aqueous solution was 40wt%, the alginate was sodium alginate, and the content of sodium alginate in the modified solution was 10wt%; the amino compound was 2-aminothiazole hydrochloride, amino-(2,4-dihydroxy-phenyl)-acetic acid and calcium cyclamate, the usage amount of 2-aminothiazole hydrochloride was 20wt% of sodium alginate, the usage amount of amino-(2,4-dihydroxy-phenyl)-acetic acid was 12wt% of sodium alginate, and the usage amount of calcium cyclamate was 3wt% of sodium alginate.

[0067] Preparation method of a microbial agent

[0068] This comparative example is different from Example 1 in the preparation of the modified solution.

[0069] Preparation of the modified solution: the alginate and the amino compound were mixed in the ethanol aqueous solution to obtain the modified solution. The content of ethanol in the ethanol aqueous solution was 40wt%, the alginate was sodium alginate, and the content of sodium alginate in the modified solution was 10wt%; the amino compound was 2-aminothiazole hydrochloride, the usage amount of 2-aminothiazole hydrochloride was 20wt% of sodium alginate.

[0070] Preparation method of a microbial agent

[0071] This comparative example is different from Example 1 in the preparation of the modified solution.

[0072] Preparation of the modified solution: the alginate and the amino compound were mixed in the ethanol aqueous solution to obtain the modified solution. The content of ethanol in the ethanol aqueous solution was 40wt%, the alginate was sodium alginate, and the content of sodium alginate in the modified solution was 10wt%; the amino compound was amino-(2,4-dihydroxy-phenyl)-acetic acid, the usage amount of amino-(2,4-dihydroxy-phenyl)-acetic acid was 12wt% of sodium alginate.

[0073] Preparation method of a microbial agent

[0074] This comparative example is different from Example 1 in the preparation of the modified solution.

[0075] Preparation of the modified solution: the alginic acid salt and the amino compound were mixed in the ethanol aqueous solution to obtain the modified solution. The content of ethanol in the ethanol aqueous solution was 40wt%, the alginic acid salt was sodium alginate, the content of sodium alginate in the modified solution was 10wt%; the amino compound was 2-aminothiazole hydrochloride and amino-(2,4-dihydroxy-phenyl)-acetic acid, the usage amount of 2-aminothiazole hydrochloride was 5wt% of sodium alginate, the usage amount of amino-(2,4-dihydroxy-phenyl)-acetic acid was 2wt% of sodium alginate.

[0076] Test example:

[0077] 1. Electron microscope characterization

[0078] The morphology of the bioactive carbon prepared in Example 1 was characterized by SEM, and the results are shown in FIG. 1. As shown in FIG. 1, the surface of the bioactive carbon is rough and uneven, and there are large concave-convex structures and pore structures on the surface. Figure 1

[0079] 2. Storage performance test

[0080] The microbial inoculants prepared in the examples and the comparative examples were packaged with packaging bags and vacuumized, and then stored at 4℃. After 180 days of storage, the effective viable cell count in the inoculants was determined. The viable cell count was determined by dilution and coating method, and the total viable cell count in the inoculants was determined. The effective viable cell count on the 0th day was taken as 100%, and the survival rate of the effective viable cell after 180 days of testing was determined. The results are shown in Table 1. Figure 2 ​S1 is Example 1, S2 is Example 2, S3 is Example 3, S4 is Example 4, S5 is Example 5, D1 is Comparative Example 1, D2 is Comparative Example 2, and D3 is Comparative Example 3. The present application is prepared by mixing Bacillus subtilis liquid, Bacillus licheniformis liquid, and Candida lipolytica liquid to obtain a liquid microbial solution, and then mixing the liquid microbial solution with a conditioner containing coconut husk, rice husk, and bioactive carbon to obtain a microbial agent. In the preparation of the bioactive carbon, a modified solution containing sodium alginate, calcium nitrate, and peanut shells is mixed and carbonized by calcination to obtain the bioactive carbon. In the preparation of the bioactive carbon, an amino compound can also be added. The amino compound includes 2-aminothiazole hydrochloride and amino-(2,4-dihydroxy-phenyl)-acetic acid. The amino compound is mixed with sodium alginate, calcium nitrate, and peanut shells and then carbonized by calcination to obtain the bioactive carbon. After the bioactive carbon is prepared by adding 2-aminothiazole hydrochloride and amino-(2,4-dihydroxy-phenyl)-acetic acid, the bioactive carbon is applied to the preparation of the microbial agent. The obtained microbial agent has a higher effective viable bacteria survival rate after long-term storage. When only one of 2-aminothiazole hydrochloride or amino-(2,4-dihydroxy-phenyl)-acetic acid is used to obtain the bioactive carbon, the effective viable bacteria survival rate of the obtained microbial agent does not significantly improve after long-term storage. Even when both 2-aminothiazole hydrochloride and amino-(2,4-dihydroxy-phenyl)-acetic acid are used to prepare the bioactive carbon, if the amount of 2-aminothiazole hydrochloride and amino-(2,4-dihydroxy-phenyl)-acetic acid is too low, the effective viable bacteria survival rate of the obtained microbial agent does not significantly improve after long-term storage. When the amount of 2-aminothiazole hydrochloride and amino-(2,4-dihydroxy-phenyl)-acetic acid is increased within a certain range, the effective viable bacteria survival rate of the obtained microbial agent after long-term storage can be improved. In addition to 2-aminothiazole hydrochloride and amino-(2,4-dihydroxy-phenyl)-acetic acid, calcium cyclamate can also be added. After the bioactive carbon is prepared from 2-aminothiazole hydrochloride, amino-(2,4-dihydroxy-phenyl)-acetic acid, and calcium cyclamate, the effective viable bacteria survival rate of the obtained microbial agent after long-term storage is further improved.

[0081] 3. Oil degradation rate

[0082] The simulated kitchen garbage includes cabbage, rice, apple peel, fatty pork, soybean oil, sodium chloride, and water. The mass fraction of cabbage is 40 wt%, the mass fraction of rice is 20 wt%, the mass fraction of apple peel is 10 wt%, the mass fraction of fatty pork is 10 wt%, the mass fraction of soybean oil is 3 wt%, the mass fraction of sodium chloride is 0.05 wt%, and the balance is water. The cabbage, rice, apple peel, and fatty pork are all crushed.

[0083] The determination of oil in the simulated kitchen garbage adopts the method of acid hydrolysis and ether extraction. First, the simulated kitchen garbage is air-dried in a uniform mixing state. 2 g of the air-dried sample is taken, then 8 mL of water and 10 mL of concentrated hydrochloric acid are added, and the mixture is stirred and digested at 70°C. After 1 h, 10 mL of ethanol is added, then 25 mL of ether is added, and the mixture is stirred and allowed to stand until the solution is naturally layered. The upper clear liquid is taken, dried to constant weight, and weighed. The weight of the oil is recorded as the initial oil content. The microbial inoculum prepared in each example and the comparative example is added to the simulated kitchen garbage. The microbial inoculum is 250 g, and the first day of the simulated kitchen garbage is 500 g. The simulated kitchen garbage is added continuously for 7 days, 500 g per day. On the 8th day, the oil content of the treated kitchen garbage is determined, and the oil degradation rate is calculated. The results are shown in Table 1. Figure 3S1 is Example 1, S2 is Example 2, S3 is Example 3, S4 is Example 4, S5 is Example 5, D1 is Comparative Example 1, D2 is Comparative Example 2, and D3 is Comparative Example 3. The present application is prepared by mixing Bacillus subtilis liquid, Bacillus licheniformis liquid, and Candida lipolytica liquid to obtain a liquid microbial solution, and then mixing the liquid microbial solution with a conditioner containing coconut husk, rice husk, and bioactive carbon to obtain a microbial agent. In the preparation of the bioactive carbon, a modified solution containing sodium alginate, calcium nitrate, and peanut shells is mixed and carbonized by calcination to obtain the bioactive carbon. In the preparation of the bioactive carbon, an amino compound can also be added. The amino compound includes 2-aminothiazole hydrochloride and amino-(2,4-dihydroxy-phenyl)-acetic acid. The amino compound is mixed with sodium alginate, calcium nitrate, and peanut shells and then carbonized by calcination to obtain the bioactive carbon. After the bioactive carbon is prepared by adding 2-aminothiazole hydrochloride and amino-(2,4-dihydroxy-phenyl)-acetic acid, the bioactive carbon is applied to the preparation of the microbial agent, and the obtained microbial agent has a higher oil degradation rate. When only one of 2-aminothiazole hydrochloride or amino-(2,4-dihydroxy-phenyl)-acetic acid is used to obtain the bioactive carbon, the oil degradation rate of the obtained microbial agent does not significantly improve. Even if both 2-aminothiazole hydrochloride and amino-(2,4-dihydroxy-phenyl)-acetic acid are used to prepare the bioactive carbon, the oil degradation rate of the obtained microbial agent does not significantly improve when the amount of 2-aminothiazole hydrochloride and amino-(2,4-dihydroxy-phenyl)-acetic acid is too low. When the amount of 2-aminothiazole hydrochloride and amino-(2,4-dihydroxy-phenyl)-acetic acid is increased, the oil degradation rate of the obtained microbial agent can be improved. In addition to 2-aminothiazole hydrochloride and amino-(2,4-dihydroxy-phenyl)-acetic acid, calcium cyclamate can also be added. After the bioactive carbon is prepared from 2-aminothiazole hydrochloride, amino-(2,4-dihydroxy-phenyl)-acetic acid, and calcium cyclamate, the oil degradation rate of the obtained microbial agent is further improved.

[0084] The above-described examples and / or embodiments are merely used to illustrate the preferred examples and / or embodiments of the present application and do not limit the embodiments of the present application in any form. Any person skilled in the art can make some changes or modifications to other equivalent examples without departing from the scope of the technical means disclosed in the present application, but should be considered as the same technology or embodiment as the present application.

[0085] The principles and implementation manners of the present application are described herein by using specific examples, and the above example descriptions are only used to help understand the method of the present application and its core idea. The above descriptions are only preferred embodiments of the present application, and it should be pointed out that, due to the limited nature of the language expression, there are objectively infinite specific structures, and for ordinary skilled persons in the technical field, some improvements, refinements or changes can be made without departing from the principles of the present application, and the above technical features can also be combined in an appropriate manner; these improvements, refinements, changes or combinations, or the direct application of the inventive concept and technical solution to other occasions without improvement, shall be regarded as the protection scope of the present application.

Claims

1. A microbial inoculant, comprising: The liquid bacterial solution and the conditioner are used in an amount of 50-100 wt% of the conditioner. The conditioner includes bioactive carbon containing alginate and calcium carbides. The bioactive carbon also contains amino carbides and peanut shell carbides. The conditioning agent also includes coconut coir and rice husk; the amount of rice husk used is 40-60 wt% of coconut coir; or the amount of bio-activated carbon used is 80-120 wt% of coconut coir. The liquid bacterial solution was prepared by mixing Bacillus subtils solution, Bacillus licheniformis solution, and Candida lipolytica solution; the preservation number of Bacillus subtils is GDMCC 1.5711, the preservation number of Bacillus licheniformis is GDMCC 1.5713, and the preservation number of Candida lipolytica is GDMCC 2.

3. In the preparation of bioactive carbon, peanut shells are cleaned, crushed, and sieved to obtain peanut shell powder. Then, a modification solution and calcium nitrate are added, and the mixture is allowed to stand at 20-40℃ for 6-24 hours. After drying and calcination, bioactive carbon is obtained. In the preparation of the modification solution, alginate is added to an ethanol-water solution and mixed to obtain the modification solution. The alginate is sodium alginate, and the content of sodium alginate in the modification solution is 5-20 wt%. Amino compounds are added during the preparation of the modified solution. The amino compounds are 2-aminothiazole hydrochloride and amino-(2,4-dihydroxy-phenyl)-acetic acid. The amount of 2-aminothiazole hydrochloride used is 10-30 wt% of sodium alginate, and the amount of amino-(2,4-dihydroxy-phenyl)-acetic acid used is 4-24 wt% of sodium alginate. The amount of modified solution used is measured by the amount of alginate, which is 10-40 wt% of peanut shell powder.

2. A method for preparing the microbial inoculant as described in claim 1, comprising: The liquid bacterial solution is mixed with the conditioner to prepare a microbial inoculum; The amount of liquid bacterial solution used is 50-100 wt% of the conditioner; the conditioner includes bio-activated carbon, which is prepared by mixing modified solution, calcium nitrate and peanut shells and then carbonizing them. The modified solution contains sodium alginate, and the content of sodium alginate in the modified solution is 5-20 wt%.

3. The method for preparing a microbial inoculant according to claim 2, characterized in that, The solvent of the modified solution is an aqueous ethanol solution, and the content of ethanol in the aqueous ethanol solution is 30-70 wt%.

4. The method for preparing a microbial inoculant according to claim 2, characterized in that, The liquid bacterial solution is composed of Bacillus subtilis solution, Bacillus licheniformis solution and Candida lipolyticis solution, which are mixed in a volume ratio of 1:0.1-10:0.1-10.

5. The use of the microbial agent as described in claim 1 in the degradation of kitchen waste.

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