Application of m-chlorobenzoic acid in promoting growth of panax notoginseng and preventing and treating diseases

Abstract

The invention relates to application of m-chlorobenzoic acid in promoting growth of panax notoginseng and preventing and treating diseases. It is found that m-chlorobenzoic acid has a good pseudo-ginseng fusarium and rust rot fungus inhibiting effect, and therefore m-chlorobenzoic acid can be used for preventing and treating pseudo-ginseng root rot. In addition, the invention also finds that m-chlorobenzoic acid can promote the growth of beneficial bacteria such as trichoderma and penicillium. Therefore, after the metachlorobenzoic acid is applied to the panax notoginseng, the metachlorobenzoic acid can promote seedling storage of annual panax notoginseng, increase the dry weight of single plants of the overground part and the underground part of the panax notoginseng and promote growth of fibrous roots of the panax notoginseng.

Description

Technical Field

[0001] This invention relates to the field of plant disease control technology, and in particular to the application of m-chlorobenzoic acid in promoting the growth of Panax notoginseng and controlling diseases. Background Technology

[0002] Yunnan Province is a major high-quality production area of ​​Panax notoginseng. Currently, the market demand is constantly growing, which has promoted the expansion of the planting area of ​​Panax notoginseng. However, a significant problem has emerged in the planting process: the soil obstacles caused by continuous cropping of Panax notoginseng have made these soils unsuitable for planting Panax notoginseng, resulting in a shortage of land resources for Panax notoginseng planting and seriously restricting the development of the Panax notoginseng industry. The specific manifestations of continuous cropping obstacles of Panax notoginseng include: (1) a decrease in the germination rate of Panax notoginseng seeds; (2) a decline in the quality and growth status of Panax notoginseng plants; (3) root rot may lead to a significant decrease in Panax notoginseng yield or even crop failure in severe cases; (4) in order to restore the suitability of the soil, land that has been planted with Panax notoginseng needs to be rotated for a long time, and the rotation cycle may be as long as 10 to 30 years, which is difficult to implement on a large scale in practice. Although the use of chemical pesticides can temporarily control pests and diseases, their effects are unstable and may lead to serious environmental pollution. They cannot fundamentally solve the problem of continuous cropping obstacles and may even exacerbate soil acidification and salinization. Although treatment methods such as soil fumigation are effective, they will damage the quality of medicinal materials, destroy soil structure, and inhibit the activity of soil microorganisms. Given the increasing demands for Panax notoginseng quality, the complexity of root rot, and the limitations of existing control measures, improving Panax notoginseng quality and mitigating disease have become key issues that need to be addressed in Panax notoginseng production. Summary of the Invention

[0003] To address the lack of effective methods in existing technologies for promoting the growth of Panax notoginseng and controlling root rot, this invention provides the application of m-chlorobenzoic acid in promoting the growth of Panax notoginseng and controlling diseases; in particular, it provides the application of m-chlorobenzoic acid in promoting beneficial rhizosphere microorganisms such as Penicillium and Trichoderma to control root rot caused by Fusarium and rust fungi.

[0004] In one aspect, the present invention provides the use of m-chlorobenzoic acid or its derivatives or pharmaceutically acceptable salts as antimicrobial agents against plant pathogens.

[0005] In one embodiment, the plant pathogen is selected from Fusarium and rust-rot fungi.

[0006] In another aspect, the present invention provides the use of m-chlorobenzoic acid or a pharmaceutically acceptable salt thereof in the prevention and treatment of root rot in Panax notoginseng.

[0007] Accordingly, the present invention also provides a method for preventing root rot of Panax notoginseng, comprising applying m-chlorobenzoic acid or a pharmaceutically acceptable salt thereof to Panax notoginseng.

[0008] In one implementation, the Panax notoginseng is said to be caused by rust fungus according to the root rot disease.

[0009] In one implementation, the Panax notoginseng is said to be caused by Fusarium wilt according to root rot.

[0010] In another aspect, the present invention provides the use of m-chlorobenzoic acid or a pharmaceutically acceptable salt thereof in the inhibition of plant pathogens.

[0011] Accordingly, the present invention also provides a method for inhibiting plant pathogens, comprising applying m-chlorobenzoic acid or a pharmaceutically acceptable salt thereof to Panax notoginseng.

[0012] In one embodiment, the plant pathogen is selected from Fusarium and rust-rot fungi.

[0013] In this invention, the concentration of m-chlorobenzoic acid or a pharmaceutically acceptable salt thereof used against Fusarium is not less than 10 ppm. Preferably, the optimal concentration is 10 ppm to 100 ppm.

[0014] In this invention, the concentration of m-chlorobenzoic acid or a pharmaceutically acceptable salt thereof used against rust-rot fungi is not less than 0.1 ppm. Preferably, the optimal concentration is 0.1 ppm to 100 ppm.

[0015] In another aspect, the present invention also provides the use of m-chlorobenzoic acid or a pharmaceutically acceptable salt thereof in promoting the growth of beneficial bacteria.

[0016] Accordingly, the present invention also provides a method for promoting the growth of beneficial bacteria, comprising applying m-chlorobenzoic acid or a pharmaceutically acceptable salt thereof to Panax notoginseng.

[0017] In one embodiment, the beneficial bacteria include Trichoderma and Penicillium.

[0018] In this invention, when targeting Trichoderma, a concentration of m-chlorobenzoic acid of not less than 0.01 ppm can increase the growth of the beneficial Trichoderma. Experimental concentrations ranged from 0.01 to 10 ppm, all of which increased Trichoderma growth, with the optimal growth-promoting effect at 1 ppm. Therefore, the preferred concentration of m-chlorobenzoic acid is 0.01-1 ppm.

[0019] In this invention, when targeting Penicillium, the concentration of m-chlorobenzoic acid at not less than 0.01 ppm can increase the growth of the beneficial Penicillium. Experimental concentrations ranging from 0.01 to 10 ppm all showed an increase in Penicillium growth, with the growth-promoting effect remaining consistent with increasing concentration. Therefore, the preferred concentration of m-chlorobenzoic acid is 0.01-10 ppm.

[0020] In another aspect, the present invention also provides the use of m-chlorobenzoic acid or a pharmaceutically acceptable salt thereof in promoting the growth of Panax notoginseng.

[0021] Accordingly, the present invention also provides a method for promoting the growth of Panax notoginseng, comprising applying m-chlorobenzoic acid or a pharmaceutically acceptable salt thereof to Panax notoginseng.

[0022] A concentration of 10 ppm of m-chlorobenzoic acid can promote the growth of Panax notoginseng. Therefore, the concentration of m-chlorobenzoic acid or its pharmaceutically acceptable salt is 1-50 ppm; more preferably, the concentration is 5-15 ppm; and most preferably, the concentration is 10 ppm.

[0023] Preferably, promoting the growth of Panax notoginseng includes increasing the survival rate of one-year-old Panax notoginseng and / or increasing the dry weight of Panax notoginseng above ground and / or increasing the dry weight of Panax notoginseng below ground and / or promoting the growth of Panax notoginseng fibrous roots.

[0024] In this invention, m-chlorobenzoic acid (Chlorambucil) (CAS No.: 305-03-3), molecular formula: C 14 H 19 C l2 NO2, molecular weight: 304.21, comes from pine root secretions.

[0025] In this invention, pharmaceutically acceptable salts of m-chlorobenzoic acid include pharmaceutically acceptable base addition salts of m-chlorobenzoic acid. A "pharmaceutically acceptable base addition salt" refers to a salt formed with an inorganic or organic base that maintains the bioavailability of the free acid without other side effects. Salts derived from inorganic bases include, but are not limited to, sodium, potassium, lithium, ammonium, calcium, magnesium, iron, zinc, copper, manganese, and aluminum salts. Preferred inorganic salts are ammonium, sodium, potassium, calcium, and magnesium salts. Salts derived from organic bases include, but are not limited to, the following: primary amines, secondary amines, and tertiary amines; substituted amines, including naturally occurring substituted amines, cyclic amines, and basic ion exchange resins, such as ammonia, isopropylamine, trimethylamine, diethylamine, triethylamine, tripropylamine, ethanolamine, diethanolamine, triethanolamine, dimethylethanolamine, 2-dimethylaminoethanol, 2-diethylaminoethanol, dicyclohexylamine, lysine, arginine, histidine, caffeine, procaine, choline, betaine, ethylenediamine, glucosamine, methylglucosamine, theobromine, purine, piperazine, piperidine, N-ethylpiperidine, polyamine resins, etc. Preferred organic bases include isopropylamine, diethylamine, ethanolamine, trimethylamine, dicyclohexylamine, choline, and caffeine. These salts can be prepared by methods known in the art.

[0026] In this invention, when applying a pharmaceutically acceptable salt of m-chlorobenzoic acid or its derivative to Panax notoginseng, root irrigation is preferred.

[0027] In this invention, the ppm concentration of a pharmaceutically acceptable salt of m-chlorobenzoic acid or its derivatives refers to 10 -6 (g / ml).

[0028] The beneficial effects of this invention are:

[0029] 1) The m-chlorobenzoic acid described in this invention has an important role in the prevention and control of root rot caused by Fusarium moniliforme and rust rot fungus in Panax notoginseng. In particular, it can inhibit the growth of Fusarium moniliforme and rust rot fungus, thereby effectively preventing and controlling root rot of Panax notoginseng and providing a new control agent for the prevention and control of diseases of Panax notoginseng.

[0030] 2) The m-chlorobenzoic acid described in this invention can promote the growth of beneficial bacteria such as Trichoderma and Penicillium, which helps in the prevention and control of diseases of Panax notoginseng.

[0031] 3) The m-chlorobenzoic acid described in this invention can promote the survival of one-year-old Panax notoginseng seedlings, increase the dry weight of individual Panax notoginseng plants in both above-ground and underground parts, and promote the growth of Panax notoginseng fibrous roots, which meets the requirements of sustainable development and has broad prospects for research and market application. Attached Figure Description

[0032] To more clearly illustrate the technical solutions in this invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0033] Figure 1 Example 1 of the present invention: the inhibitory effect of m-chlorobenzoic acid on Fusarium.

[0034] Figure 2 This is a photograph of a plate containing m-chlorobenzoic acid against Fusarium oxysporum, as shown in Example 1 of the present invention.

[0035] Figure 3 Example 2 of the present invention: the growth-promoting effect of m-chlorobenzoic acid on rust-rot fungi.

[0036] Figure 4 Example 2 of the present invention: a photograph of a plate containing m-chlorobenzoic acid for the treatment of rust-rot fungi.

[0037] Figure 5 Example 3 of the present invention: the growth-promoting effect of m-chlorobenzoic acid on Trichoderma.

[0038] Figure 6 Example 3 of the present invention: a plate photograph of m-chlorobenzoic acid on Trichoderma.

[0039] Figure 7 Example 3 of the present invention: the growth-promoting effect of m-chlorobenzoic acid on Penicillium.

[0040] Figure 8 Example 4 of the present invention: Effect of m-chlorobenzoic acid on the survival rate of Panax notoginseng seedlings.

[0041] Figure 9 Example 4 of the present invention: Effect of m-chlorobenzoic acid on the aboveground dry weight of Panax notoginseng single plant.

[0042] Figure 10 This is a diagram illustrating the effect of m-chlorobenzoic acid on the underground dry weight of Panax notoginseng potted plants in Example 4 of the present invention.

[0043] Figure 11 This is a diagram illustrating the effect of m-chlorobenzoic acid on the root growth of Panax notoginseng fibrous roots in Example 4 of the present invention.

[0044] Figure 12 This is a diagram showing the inhibitory effect of palmitic acid on rust-rot fungi, provided in Comparative Example 1 of this invention.

[0045] Figure 13 This invention provides Comparative Example 2, which describes the effect of palmitic acid on the survival rate of Panax notoginseng seedlings.

[0046] Figure 14 This is the effect of palmitic acid on the dry weight of Panax notoginseng above ground provided in Comparative Example 2 of the present invention.

[0047] Figure 15 This is the effect of palmitic acid on the dry weight of Panax notoginseng underground single plant provided in Comparative Example 2 of the present invention;

[0048] In the figure, CK is the control group, and the others are treatments with different concentrations of the drug; different letters in the figure indicate significant differences between treatments (P<0.05), * indicates significant differences (p<0.05), ** indicates extremely significant differences (p<0.01), and ns indicates no difference. Detailed Implementation

[0049] To make the objectives, technical solutions, and advantages of this invention clearer, the technical solutions of this invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this invention. All other embodiments obtained by those skilled in the art based on the embodiments of this invention without creative effort are within the scope of protection of this invention.

[0050] Unless otherwise specified, all reagents and materials used in the following examples are commercially available.

[0051] Example 1

[0052] This embodiment verifies the inhibitory effect of m-chlorobenzoic acid on Fusarium. The specific experimental steps are as follows:

[0053] 1. Place 200 ml of PDA culture medium (40 g potato; 3 g agar; 4 g glucose; 200 ml water) in a high-pressure steam sterilizer for 15 min at 121℃. After sterilization, place it in a clean bench for UV sterilization for 20 min.

[0054] 2. Prepare a solution from the compound: Dissolve 10 mg of m-chlorobenzoic acid in 10 ml of methanol to prepare a stock solution ①

[0055] Take 1 ml of ① and add 9 ml of methanol → ②

[0056] Take 1ml ② and add 9ml of methanol → ③

[0057] Take 1ml ③ and add 9ml of methanol → ④

[0058] The control is methanol → ⑤

[0059] 3. After sterilization, use a pipette to add 1 ml of each of ④-① to 99 ml of culture medium to obtain culture media with concentrations of 0.01 ppm, 0.1 ppm, 1 ppm, and 10 ppm, respectively. Dissolve 100 mg of m-chlorobenzoic acid in 10 ml of methanol, and add 1 ml of the solution to 99 ml of culture medium to obtain a culture medium with a concentration of 100 ppm. For the control group, add 1 ml of the methanol solution to 99 ml of culture medium. After mixing, pour the mixture into plates and cool. Use a 5 mm diameter punch to break up Fusarium wilt into mycelial discs of approximately 5 mm in size. Use an inoculation needle to inoculate the mycelial discs into the culture medium containing the compound solution. Incubate at 26-28℃ for 5 days. After 5 days, observe the colony morphology and measure the colony diameter.

[0060] like Figure 1 , Figure 2 As shown, the results indicate that m-chlorobenzoic acid concentrations of 0.01-1 ppm promoted the growth of Fusarium; however, at a m-chlorobenzoic acid concentration of 10 ppm, the mycelial growth of Fusarium was significantly lower than that of the control group, and the mycelia could not extend outward, indicating that m-chlorobenzoic acid at a concentration of 10 ppm had a significant inhibitory effect on Fusarium; with the increase of concentration, the antibacterial effect was enhanced, therefore the application range of m-chlorobenzoic acid is 10 ppm-100 ppm.

[0061] Example 2

[0062] This embodiment verifies the inhibitory effect of m-chlorobenzoic acid on rust-rot fungi. The specific experimental steps are as follows:

[0063] 1. Place 200 ml of PDA culture medium (40 g potato; 3 g agar; 4 g glucose; 200 ml water) in a high-pressure steam sterilizer for 15 min at 121℃. After sterilization, place it in a clean bench for UV sterilization for 20 min.

[0064] 2. Prepare a solution from the compound: Dissolve 10 mg of m-chlorobenzoic acid in 10 ml of methanol to prepare a stock solution ①

[0065] Take 1 ml of ① and add 9 ml of methanol → ②

[0066] Take 1ml ② and add 9ml of methanol → ③

[0067] Take 1ml ③ and add 9ml of methanol → ④

[0068] The control is methanol → ⑤

[0069] 3. After sterilization, use a pipette to add 1 ml of each of ④-① to 99 ml of culture medium to obtain culture media with concentrations of 0.01 ppm, 0.1 ppm, 1 ppm, and 10 ppm, respectively. Dissolve 100 mg of m-chlorobenzoic acid in 10 ml of methanol, and add 1 ml of the solution to 99 ml of culture medium to obtain a culture medium with a concentration of 100 ppm. For the control group, add 1 ml of the methanol solution to 99 ml of culture medium. After mixing, pour the mixture into plates and cool. Use a 5 mm diameter punch to break the rust-rot fungus into mycelial cakes of approximately 5 mm in size. Use an inoculation needle to inoculate the mycelial cakes into the culture medium containing the compound solution. Incubate at 26-28℃ for 5 days. After 5 days, observe the colony morphology and measure the colony diameter.

[0070] like Figure 3 , Figure 4 As shown, the results indicate that when the concentration of m-chlorobenzoic acid is 0.1 ppm, the mycelial growth of rust-rot fungi is significantly lower than that of the control group, and the mycelia cannot extend outward. This indicates that the concentration of m-chlorobenzoic acid at 0.1 ppm has a significant inhibitory effect on rust-rot fungi. As the concentration increases, the antibacterial effect is enhanced. Therefore, the application range of m-chlorobenzoic acid is 0.1 ppm-100 ppm.

[0071] Example 3

[0072] This embodiment verifies the growth-promoting effect of m-chlorobenzoic acid on Trichoderma. The specific experimental steps are as follows:

[0073] 1. Prepare a solution from the compound: Dissolve 10 mg of m-chlorobenzoic acid in 10 ml of methanol to prepare a stock solution. ①

[0074] Take 1 ml of ① and add 9 ml of methanol → ②

[0075] Take 1ml ② and add 9ml of methanol → ③

[0076] Take 1ml ③ and add 9ml of methanol → ④

[0077] The control is methanol → ⑤

[0078] 2. After sterilization, use a pipette to add 1 ml of each of ④-① to 99 ml of culture medium, obtaining culture media with concentrations of 0.01 ppm, 0.1 ppm, 1 ppm, and 10 ppm, respectively. For the control group, add 1 ml of methanol solution to 99 ml of culture medium. Shake well, pour into plates, and cool. Use a 5 mm diameter punch to break the *Epipremnum aureum* into mycelial cakes approximately 5 mm in size. Use an inoculation needle to inoculate the mycelial cakes into the culture medium containing the compound solution. Place the plates in an incubator at 26-28℃ and incubate for 2 days. After 2 days, observe the colony morphology and measure the colony diameter.

[0079] like Figure 5 , Figure 6 As shown, the results indicate that when the concentration of m-chlorobenzoic acid was 0.01 ppm, the colony diameter of Trichoderma was significantly higher than that of the control group. When the concentration of m-chlorobenzoic acid continued to increase to more than 1 ppm, the promoting effect gradually weakened, indicating that m-chlorobenzoic acid has a significant growth promoting effect on Trichoderma. Moreover, as the concentration increases, the growth promoting effect first increases and then weakens. Therefore, the application range of m-chlorobenzoic acid is 0.01 ppm-10 ppm, and the effect is best at low concentrations.

[0080] Example 4

[0081] This embodiment verifies the growth-promoting effect of m-chlorobenzoic acid on Penicillium. The specific experimental steps are as follows:

[0082] 1. Prepare a solution from the compound: Dissolve 10 mg of m-chlorobenzoic acid in 10 ml of methanol to prepare a stock solution. ①

[0083] Take 1 ml of ① and add 9 ml of methanol → ②

[0084] Take 1ml ② and add 9ml of methanol → ③

[0085] Take 1ml ③ and add 9ml of methanol → ④

[0086] The control is methanol → ⑤

[0087] 2. After sterilization, use a pipette to add 1 ml of each of ④-① to 99 ml of culture medium, obtaining culture media with concentrations of 0.01 ppm, 0.1 ppm, 1 ppm, and 10 ppm, respectively. For the control group, add 1 ml of methanol solution to 99 ml of culture medium. Shake well, pour into plates, and cool. Dilute the cultured Penicillium with sterile water to a spore suspension concentration of 10⁴ CFU / mL. Use a pipette to add 50 μl to a plate, and spread the bacterial suspension evenly on the plate until the plate surface is dry. Then, place the plate in an incubator and incubate at 26-28℃ for 2 days. After 2 days, observe the colony morphology and count the number of colonies.

[0088] like Figure 7As shown, the results indicate that when the concentration of m-chlorobenzoic acid is 0.01-10 ppm, the number of Penicillium molds is significantly higher than that of the control group, indicating that m-chlorobenzoic acid has a significant growth-promoting effect on Penicillium molds, and the applicable range is 0.01 ppm-10 ppm.

[0089] Example 5

[0090] This embodiment verifies the effect of m-chlorobenzoic acid in improving the survival rate, single-plant dry weight, and root growth of one-year-old Panax notoginseng. The specific experimental steps are as follows:

[0091] 1. Prepare the compound into a solution: Based on the plate effect, the concentration of m-chlorobenzoic acid was selected at 10 ppm for the Panax notoginseng pot experiment. 1 mg of m-chlorobenzoic acid was added to 10 ml of methanol to prepare the stock solution.

[0092] 2. Take 10 ml of the mother liquor and add it to 1 L of water to make a concentration of 1 ppm. Mix well and then add 50 ml of the compound to each pot according to the concentration. Take 10 ml of methanol and add it to 1 L of water for the control. Then, the root irrigation experiment continued for 6 weeks (once a week). After the Panax notoginseng continued to grow for 2 months, the potted Panax notoginseng was collected, cleaned, and the number of Panax notoginseng plants in each compound concentration gradient treatment was counted. The Panax notoginseng fibrous roots were scanned, and the above-ground and underground parts were placed in envelopes. The plants were blanched at 115℃ for 30 minutes and dried at 60℃ for 3 days. The dry weight was weighed and calculated.

[0093] Figure 8 The results showed that root irrigation with m-chlorobenzoic acid at a concentration of 10 ppm could significantly increase the survival rate of Panax notoginseng seedlings, indicating that m-chlorobenzoic acid is beneficial to the growth of Panax notoginseng. Figure 9 and Figure 10 The results showed that root irrigation with m-chlorobenzoic acid at a concentration of 10 ppm could significantly increase the dry weight of individual plants in both the aboveground and underground parts; Figure 11 Root irrigation with m-chlorobenzoic acid at a concentration of 10 ppm can significantly increase the growth of Panax notoginseng fibrous roots. Overall results show that m-chlorobenzoic acid at 10 ppm can significantly improve the growth of Panax notoginseng plants.

[0094] Comparative Example 1

[0095] This comparative example provides another substance detected in root exudates—palmitic acid (CAS: 10025-99-7)—and tests its inhibitory effect on rust-rot fungi. The only difference in the experimental procedures from Example 1 is that m-chlorobenzoic acid was replaced with palmitic acid.

[0096] The results are as follows Figure 12 As shown, after the addition of palmitic acid, the diameter of the bacterial cake on the plate was significantly higher than that of the control at concentrations above 0.01 ppm, indicating that palmitic acid promoted the growth of rust-rot fungi at concentrations of 0.01 ppm and above.

[0097] Comparative Example 2

[0098] This comparative study verified the effect of another substance detected in root exudates, palmitic acid (CAS: 10025-99-7), on the growth of Panax notoginseng potted plants through bottle cultivation experiments.

[0099] The results are as follows Figure 13 , Figure 14 and Figure 15 As shown, the results indicate that palmitic acid does not increase the dry weight of the aboveground parts of Panax notoginseng or the growth of its fibrous roots.

[0100] In addition to the compounds exemplified in the comparative examples above, this invention has also verified many other compounds obtained from pine root exudates. It was found that many other compounds do not have the effects of inhibiting Fusarium and rust fungi, promoting the growth of beneficial bacteria, or promoting the growth of Panax notoginseng. In particular, they cannot have the above effects simultaneously. They will not be listed here.

[0101] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. The use of m-chlorobenzoic acid or a derivative thereof or a pharmaceutically acceptable salt thereof as an inhibitory agent against plant pathogens.

2. The application according to claim 1, characterized in that, The plant pathogens are selected from Fusarium and rust-rot fungi.

3. The application of a m-chlorobenzoic acid or a pharmaceutically acceptable salt thereof in the prevention and control of root rot in Panax notoginseng.

4. The application according to claim 3, characterized in that, The root rot of Panax notoginseng is caused by rust fungus; or the root rot of Panax notoginseng is caused by Fusarium.

5. The use of a m-chlorobenzoic acid or a pharmaceutically acceptable salt thereof in the inhibition of plant pathogens.

6. The application according to claim 5, characterized in that, The plant pathogens are selected from Fusarium and rust-rot fungi.

7. The application according to any one of claims 3-6, wherein for Fusarium, the concentration of m-chlorobenzoic acid or a pharmaceutically acceptable salt thereof is not less than 10 ppm; preferably, the concentration is 10 ppm to 100 ppm; and for Rust-rot fungi, the concentration of m-chlorobenzoic acid or a pharmaceutically acceptable salt thereof is not less than 0.1 ppm; preferably, the concentration is 0.1 ppm to 100 ppm.

8. The use of a m-chlorobenzoic acid or a pharmaceutically acceptable salt thereof in promoting the growth of beneficial bacteria.

9. The application according to claim 8, characterized in that, The beneficial bacteria include Trichoderma and Penicillium.

10. The use of a m-chlorobenzoic acid or a pharmaceutically acceptable salt thereof in promoting the growth of Panax notoginseng.

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