Green pesticide insecticidal composition and application thereof

Abstract

The invention belongs to the field of agricultural chemistry, and particularly relates to a green pesticide insecticidal composition and application thereof. Active components of the pesticide composition comprise veratrine and 6-methylcoumarin, and the weight ratio of the veratrine to the 6-methylcoumarin is (20: 1)-(1: 10). The insecticidal composition disclosed by the invention belongs to a plant source insecticidal composition and is environment-friendly, and the active ingredients have an obvious synergistic effect, so that the use amount of medicaments can be reduced, and the generation of drug resistance of pests can be delayed.

Description

Technical Field

[0001] This invention belongs to the field of agricultural chemistry, specifically relating to a green pesticide insecticidal composition and its application. Background Technology

[0002] Aphids are common pests in agricultural production. For a long time, aphid control has primarily relied on chemical pesticides, with neonicotinoid pesticides being the preferred choice due to their unique mechanism of action. However, the long-term use of single pesticides has led to a significant increase in aphid resistance. Implementing green pest control is a crucial means of ensuring high-quality agricultural development. Research and practice have proven that the scientific use of plant-derived pesticides is a key measure in building green plant protection technologies, offering advantages such as reduced dosage and increased efficiency.

[0003] Veratrine is a plant-derived insecticidal active substance extracted from the natural plant Veratrum nigrum. It is characterized by being pollution-free, residue-free, and having a long-lasting effect. It also enhances plant immunity, promotes crop growth, and controls both pests and diseases, making it a new generation of highly effective, low-toxicity, broad-spectrum insecticide. Coumarin compounds, as a class of plant secondary metabolites, are widely found in the roots, stems, and leaves of plants in the Asteraceae and Apiaceae families. They possess a wide range of biological activities, including antiviral, antibacterial, and anticancer properties, as well as highly effective insecticidal effects. They can serve as a potential insecticide to effectively control aphid infestations.

[0004] This invention provides a green pesticide insecticidal composition containing veratrine and coumarin compounds. Under appropriate ratios, the composition exhibits a significant synergistic effect, effectively reducing the amount of pesticide used, minimizing the environmental harm of chemical pesticides, and helping to delay the development of pesticide resistance. There are no prior art reports on the combined use of veratrine and coumarin compounds. Summary of the Invention

[0005] To address the problems existing in the prior art, the present invention provides the following technical solution:

[0006] A green pesticide insecticidal composition, the active ingredients of which include veratrine and 6-methylcoumarin, wherein the weight ratio of veratrine and 6-methylcoumarin is 20:1 to 1:10.

[0007] The insecticidal composition of the present invention comprises veratrine and 6-methylcoumarin in a weight ratio of 10:1 to 1:10. Preferably, the weight ratio of veratrine and 6-methylcoumarin is 1:5 or 1:1.

[0008] This invention provides a green pesticide insecticidal composition, further comprising agriculturally acceptable adjuvants, with the active ingredient comprising 1-20% by weight. Preferably, the active ingredient comprises 1-10% by weight. More preferably, the active ingredient comprises 1-5% by weight.

[0009] The additives described in this invention include one or more of the following: dispersants, wetting agents, emulsifiers, disintegrants, stabilizers, synergists, penetrants, or fillers.

[0010] The insecticidal composition of the present invention can be prepared into wettable powder, water-dispersible granules, or pellets.

[0011] The insecticidal composition described in this invention is used for the control of crop aphids, particularly peach aphids.

[0012] Compared with the prior art, the present invention has the following beneficial effects:

[0013] The insecticidal composition of the present invention belongs to plant-derived insecticidal compositions, which are environmentally friendly, and the active ingredients have a significant synergistic effect, which can reduce the amount of pesticide used and help delay the development of pesticide resistance in pests.

[0014] When the weight ratio of veratrine to 6-methylcoumarin is in the range of 1:10-20:1, the composition exhibits a significant synergistic effect on aphids, which can effectively reduce the dosage of active ingredients and improve the control effect. Detailed Implementation

[0015] The specific embodiments of the present invention will be described in detail below with reference to the examples, but it should be understood that the scope of protection of the present invention is not limited to the specific embodiments.

[0016] Indoor bioassay: Indoor insecticidal toxicity test of veratrine and 6-methylcoumarin combination against aphids.

[0017] Experimental subject: Peach aphid, collected from the experimental base and artificially reared for more than five generations in the insect rearing room; fourth instar nymphs of uniform size and strong vitality were selected for later use.

[0018] Experimental Methods: The insecticidal activity of the pesticide was determined using the leaf immersion method. A series of pesticide solutions of varying concentrations were prepared. Leaf discs containing peach aphids were immersed in the pesticide solution for 5 seconds, then removed. Excess pesticide on the leaf discs and around the aphids was absorbed with filter paper, and the discs were cultured under identical rearing conditions. Each treatment consisted of 30 aphids, with three replicates. Survival status was checked 48 hours after treatment. A larval death was defined as no reaction when lightly touched with a brush. The number of deaths and survivors was recorded, and the corrected mortality rate was calculated.

[0019] The corrected mortality rates for each treatment were converted into probability values, and the concentrations were converted into logarithmic values. The toxicity equation and the median LC50 of inhibition were calculated using the least squares method. The toxicity index and co-toxicity coefficient (CTC) of the agent were calculated according to Sun Yunpei's method.

[0020] When CTC ≤ 80, the composition exhibits an antagonistic effect; when 80 < CTC < 120, the composition exhibits an additive effect; and when CTC ≥ 120, the composition exhibits a synergistic effect.

[0021] Actual Toxicity Index (ATI) = (LC50 of Standard Reagent / LC50 of Test Reagent) × 100

[0022] Theoretical Toxicity Index (TTI) = Toxicity Index of Agent A × Percentage of A in the Mixture + Toxicity Index of Agent B × Percentage of B in the Mixture

[0023] Co-toxicity coefficient (CTC) = [Actual toxicity index (ATI) of mixture / Theoretical toxicity index (TTI) of mixture] × 100.

[0024] The results of the toxicity test are shown in Table 1 below.

[0025] Table 1. Indoor insecticidal toxicity determination of veratrine and 6-methylcoumarin combination against aphids.

[0026] As shown in Table 1, when the weight ratio of veratrine to 6-methylcoumarin in the insecticidal composition of the present invention is in the range of 1:20-20:1, the composition exhibits different synergistic effects on aphids. When the weight ratio of veratrine to 6-methylcoumarin is in the range of 1:10-20:1, the composition exhibits a significant synergistic effect on aphids, which can effectively reduce the dosage of active ingredients and improve the control effect.

[0027] Field efficacy testing

[0028] Experimental subject: Tobacco peach aphid

[0029] Test reagents:

[0030] 2% Veratrine·6-Methylcoumarin Wettable Powder (containing the following ingredients in weight percentage: veratrine 1%, 6-methylcoumarin 1%, calcium dodecylbenzenesulfonate 4%, sodium lignosulfonate 2%, bleaching powder BX 2%, silica 5%, kaolin to make up 100%).

[0031] 1% Veratrine Wettable Powder (containing the following ingredients in weight percentage: veratrine 1%, calcium dodecylbenzenesulfonate 4%, sodium lignosulfonate 2%, bleaching powder BX 2%, silica 5%, kaolin to make up 100%).

[0032] 1% 6-Methylcoumarin wettable powder (containing the following ingredients in weight percentage: 6-methylcoumarin 1%, calcium dodecylbenzenesulfonate 4%, sodium lignosulfonate 2%, bleaching powder BX 2%, silica 5%, kaolin to make up 100%).

[0033] Experimental Methods: The experiment was conducted in a tobacco plantation where the tobacco plants were uniformly growing. A plot-based efficacy test was performed. At the initial flowering stage of the tobacco plants, during the peak period of peach aphid infestation, a single, uniform spray was applied to the entire plant using a manual sprayer. The experiment included pesticide treatments, with four replicates per treatment, arranged in a randomized block design. Each plot was 35 m², and a blank control was included.

[0034] Five samples were taken from each plot, and 20 tobacco plants were continuously surveyed and marked at each point. The number of live insects on the tobacco plants was counted. Field surveys were conducted before and 14 days after the pesticide application. The control effect was calculated based on the insect population reduction rate after the pesticide application.

[0035] Insect population reduction rate % = (Number of insects before application - Number of insects after application) / Number of insects before application × 100

[0036] Control effect % = (Pest population reduction rate in the treatment area - Pest population reduction rate in the control area) / (100 - Pest population reduction rate in the control area) × 100.

[0037] Table 2. Field control efficacy of 2% veratrine·6-methylcoumarin wettable powder against tobacco aphid.

[0038] As shown in Table 2, compared with application alone, the 2% veratrine·6-methylcoumarin wettable powder prepared in this invention can significantly improve the field control effect of the pesticide on tobacco aphids, effectively reduce the dosage of active ingredients, and reduce pollution from chemical pesticides.

[0039] Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this invention shall be included within the scope of protection of this invention. All percentages in the formulations are by weight. The processing techniques for each formulation of the compositions of this invention are existing technologies and may vary depending on different circumstances.

Claims

1. A green pesticide insecticidal composition, characterized in that, The active ingredients include veratrine and 6-methylcoumarin, with a weight ratio of 20:1 to 1:

10.

2. The insecticidal composition according to claim 1, characterized in that, The weight ratio of veratrine to 6-methylcoumarin is 10:1 to 1:

10.

3. The insecticidal composition according to claim 2, characterized in that, The weight ratio of veratrine to 6-methylcoumarin is 1:5 and 1:

1.

4. The insecticidal composition according to any one of claims 1-3, characterized in that, It also contains agriculturally acceptable adjuvants, with the active ingredient comprising 1-20% by weight.

5. The insecticidal composition according to claim 4, characterized in that, The additives include one or more of the following: dispersants, wetting agents, emulsifiers, disintegrants, stabilizers, synergists, penetrants, or fillers.

6. The insecticidal composition according to claim 5, characterized in that, The composition can be prepared into a dosage form such as a wettable powder, a water-dispersible granule, or a pellet.

7. Use of the insecticidal composition according to any one of claims 1-6 for the control of crop aphids.

8. The use according to claim 7, characterized in that, Used for the control of peach aphids.

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