Composite peptide nutritional premixed feed capable of reducing fat deposition and preparation method of composite peptide nutritional premixed feed

Abstract

The invention discloses a composite peptide nutritional premix feed for reducing fat deposition and a preparation method thereof, and relates to the technical field of feed additives, in order to reduce fat deposition of livestock and poultry, composite peptide is added into the premix feed, and the composite peptide is a protein hydrolysate, is small in molecular weight and can be directly absorbed through an intestinal peptide transfer channel, so that the fat deposition of livestock and poultry is reduced. In order to further reduce gastrointestinal tract infection in the animal feeding process, an anti-mildew and antibacterial agent is further added, probiotics are subjected to microencapsulation treatment, and the content of free amino acid is increased, so that the content of the free amino acid is increased, and the content of the free amino acid is increased. The probiotics are prevented from being inactivated due to the influence of the mildew-proof antibacterial agent in the preservation process, the gastric acid resistance of the probiotics can be effectively improved through microencapsulation treatment, the survival rate of the probiotics in the intestinal tract is improved, the intestinal flora is improved, and the disease resistance of livestock and poultry is improved.

Description

Technical Field

[0001] This invention relates to the field of feed additive technology, specifically to a compound peptide nutritional premixed feed that reduces fat deposition and its preparation method. Background Technology

[0002] With the development of my country's animal husbandry, the country has gradually moved beyond the stage of solely pursuing fattening and has begun to focus on improving the meat quality of livestock and poultry, reducing fat deposition, and enhancing muscle quality to increase palatability. Therefore, it is necessary to improve animal feed to reduce fat deposition and improve meat quality. Summary of the Invention

[0003] The purpose of this invention is to provide a compound peptide nutritional premixed feed that reduces fat deposition and its preparation method, so as to solve the problems raised in the prior art.

[0004] To achieve the above objectives, the present invention provides the following technical solution: a compound peptide nutritional premixed feed for reducing fat deposition, having the following technical features, wherein, by weight, the compound peptide nutritional premixed feed for reducing fat deposition is composed of 0.23-1.5 parts of compound vitamins, 10-60 parts of compound peptides, 50-150 parts of probiotic microcapsules, 20-30 parts of anti-mold and antibacterial agent, and 800-1000 parts of purified water.

[0005] Furthermore, by weight, the complex vitamins consist of 0.05-0.2 parts vitamin B1, 0.1-1 part vitamin B2, 0.05-0.2 parts vitamin B6, and 0.03-0.1 parts vitamin B... 12 composition.

[0006] Furthermore, by weight, the complex peptide is composed of 5-30 parts bovine lactoferrin peptide, 2-12 parts glutathione, 2-12 parts soybean protein peptide and 1-6 parts casein peptide.

[0007] Furthermore, the preparation method of the probiotic microcapsules includes the following steps: A1. Mix skim milk with distilled water, heat to boiling for sterilization, cool to 37±0.5℃, inoculate with probiotics, and after the skim milk coagulates, continue subculturing 2-3 times. Then cool to 2-4℃, centrifuge to separate the bacteria and obtain bacterial sludge. A2. Add sodium alginate to distilled water to prepare sodium alginate aqueous solution. Mix it evenly with bacterial sludge and add it to salad oil. Add Tween 80 and continue stirring and emulsifying for 10-30 minutes. Then, add calcium chloride aqueous solution dropwise. After the addition is complete, let it stand for 30-90 minutes. Centrifuge to separate the precipitate and wash it 2-3 times with deionized water to obtain probiotic microcapsules.

[0008] Furthermore, in step A1, the probiotics are lactic acid bacteria.

[0009] Furthermore, in step A2, the amounts of each component added, by weight, are 60-85 parts sodium alginate aqueous solution, 5-20 parts bacterial mud, 60-80 parts salad oil, 0.15-0.3 parts Tween 80, and 60-80 parts calcium chloride aqueous solution. The sodium alginate aqueous solution has a sodium alginate concentration of 1.5-2 wt%, and the calcium chloride aqueous solution has a calcium chloride concentration of 2-4 wt%.

[0010] Furthermore, the preparation method of the antifungal and antibacterial agent includes the following steps: B1. After heating the attapulgite clay to 350~380℃ and calcining it for 1~2 hours, the water of crystallization and organic matter in it are removed to obtain calcined attapulgite clay. B2. Disperse calcined attapulgite clay in a 0.5-2 mol / L sulfuric acid solution, stir and react for 2-4 hours at room temperature, centrifuge to separate the precipitate, wash with deionized water until the washing solution is neutral, and dry to constant weight to obtain activated attapulgite clay. B3. Mix activated attapulgite clay with anhydrous ethanol, stir and disperse evenly, add KH550 and deionized water, adjust the pH to 4-5, stir and react for 2-6 hours, centrifuge to separate the precipitate, wash with anhydrous ethanol 2-3 times, disperse the precipitate again in dimethyl sulfoxide, sonicate to disperse evenly, add 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and 2,5-thiophene dicarboxylic acid, heat to 85-105℃, stir and react for 2-4 hours, centrifuge to separate the precipitate, dry to constant weight to obtain thiophene-modified attapulgite clay; B4. Thiophene-modified attapulgite clay is dispersed in an aqueous acetic acid solution with a concentration of 1-2 wt%. After ultrasonic dispersion, chitosan oligosaccharide and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide are added. The mixture is heated to 75-85℃ and stirred for 12-24 hours. The precipitate is then separated by centrifugation and redispersed in deionized water. Acetic acid is added to adjust the pH to 4.5-5.5. Ferrous sulfate, hydrogen peroxide, and ascorbic acid are added. The mixture is heated to 40-42℃ and stirred for 0.5-1 hour. Naringin is then added. The mixture is kept at this temperature under a nitrogen atmosphere for 12-24 hours. The precipitate is then separated by centrifugation to obtain an antifungal and antibacterial agent.

[0011] Furthermore, in step B3, the amounts of each component added, by weight, are 10 parts activated attapulgite clay, 50-100 parts anhydrous ethanol, 5-15 parts KH550, 10-20 parts deionized water, 0.03-0.05 parts 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, and 0.5-3 parts 2,5-thiophene dicarboxylic acid.

[0012] Furthermore, in step B4, the amounts of each component added, by weight, are 10 parts of thiophene-modified attapulgite clay, 5-20 parts of chitosan oligosaccharide, 0.03-0.06 parts of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, 0.05-0.15 parts of ferrous sulfate, 15-30 parts of hydrogen peroxide, 0.1-0.3 parts of ascorbic acid, and 0.3-3 parts of naringenin.

[0013] Furthermore, a method for preparing a compound peptide nutritional premixed feed that reduces fat deposition includes the following steps: The compound peptides, antifungal and antibacterial agents, and purified water are mixed and dispersed by ultrasonic vibration. Probiotic microcapsules and compound vitamins are then added and stirred until evenly mixed. The mixture is then packaged to obtain a compound peptide nutritional premixed feed that reduces fat deposition.

[0014] Compared with the prior art, the beneficial effects of the present invention are: To reduce fat deposition in livestock and poultry, this invention provides a premixed feed containing complex peptides and active probiotics. The added complex peptides are protein hydrolysates with small molecular weights, allowing for direct absorption through intestinal peptide transport channels. This avoids competition from free amino acids, significantly improving protein synthesis efficiency and increasing muscle mass. Furthermore, this invention specifies the types of complex peptides. The added bovine lactoferrin peptide can activate macrophage phagocytic function, promote lymphocyte proliferation, and increase immunoglobulins. The small molecule protein peptides can also effectively promote the proliferation of probiotics and lactic acid bacteria, improving the balance of intestinal flora and further reducing gastrointestinal infections and diarrhea rates in livestock and poultry. Based on this, in order to reduce the number of times feed is added daily, the present invention adds an anti-mold and antibacterial agent to the premixed feed prepared by the present invention, so that the premixed feed prepared by the present application can still inhibit the growth of pathogenic bacteria in a certain humid environment after being mixed with feed, thus preventing the feed from spoiling. In preparing the antifungal and antibacterial agent, this invention uses attapulgite clay as a raw material, which is calcined and acidified to expand the interlayer spacing and increase its surface area. In subsequent steps, KH550 hydrolysis is used to further introduce amino groups onto the surface of the attapulgite clay. Then, it is mixed with 2,5-thiophene dicarboxylic acid containing antibacterial activity, thereby introducing thiophene groups and free carboxyl groups onto the surface of the attapulgite clay. This is then further mixed with chitosan oligosaccharides, and the amidation reaction between carboxyl and amino groups is used to load chitosan oligosaccharides with antibacterial activity onto the surface of the attapulgite clay. Finally, it is treated with hydrogen peroxide... Under the action of ascorbic acid, hydrogen peroxide decomposes to generate free radicals, thereby loading the natural antibacterial substance naringenin containing polyphenol groups onto the surface of attapulgite clay, enhancing its antibacterial and antifungal activity. In order to avoid the influence of the antifungal and antibacterial agent prepared in this invention on the probiotics in this application, the probiotics are also microencapsulated. This prevents the probiotics from being inactivated by the antifungal and antibacterial agent during storage. Furthermore, microencapsulation can effectively improve the probiotics' resistance to gastric acid, increase their survival rate in the intestine, effectively improve the preservation of feed in hot and humid weather, and effectively reduce the number of times feed is added daily. Detailed Implementation

[0015] Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this invention.

[0016] Example 1. A method for preparing a compound peptide nutritional premix feed that reduces fat deposition, comprising the following steps: By weight, 10 parts of compound peptide, 20 parts of anti-mold and antibacterial agent and 800 parts of purified water are mixed and dispersed by ultrasonic vibration. After being mixed evenly, 50 parts of probiotic microcapsules and 0.2 parts of compound vitamins are added and stirred evenly. After being packaged, compound peptide nutritional premixed feed that reduces fat deposition is obtained. The complex peptide is composed of 5 parts bovine lactoferrin peptide, 2 parts glutathione, 2 parts soybean protein peptide and 1 part casein peptide. The complex vitamin consists of 0.05 parts vitamin B1, 0.1 parts vitamin B2, 0.05 parts vitamin B6, and 0.03 parts vitamin B1. 12 composition; The preparation method of the probiotic microcapsules includes the following steps: A1. Skim milk and distilled water were mixed to prepare a skim milk with a concentration of 11wt%. The mixture was heated to boiling and kept at that temperature for 15 minutes for sterilization. After cooling to 37±0.5℃, lactic acid bacteria were inoculated into it. After the skim milk coagulated, it was passaged 3 times. After cooling to 4℃, it was centrifuged to obtain bacterial sludge. A2. Add sodium alginate to distilled water to prepare a sodium alginate aqueous solution with a concentration of 1.5 wt%. Mix 60 parts of sodium alginate aqueous solution with 5 parts of bacterial sludge by weight, add it to 60 parts of salad oil, add 0.15 parts of Tween 80, continue stirring and emulsifying for 30 minutes, then add 60 parts of calcium chloride aqueous solution dropwise. After the addition is completed, let it stand for 90 minutes, centrifuge to separate the precipitate, and wash it twice with deionized water to obtain probiotic microcapsules. The preparation method of the antifungal and antibacterial agent includes the following steps: B1. After heating the attapulgite clay to 350℃ and calcining it for 1 hour, the water of crystallization and organic matter in it are removed to obtain calcined attapulgite clay. B2. Calcined attapulgite clay was dispersed in a 0.5 mol / L sulfuric acid solution. After stirring and reacting for 2 hours at room temperature, the precipitate was separated by centrifugation, washed with deionized water until the washing solution was neutral, and then dried to constant weight to obtain activated attapulgite clay. B3. By weight, 10 parts of activated attapulgite clay and 50 parts of anhydrous ethanol were mixed and stirred until evenly dispersed. Then, 5 parts of KH550 and 10 parts of deionized water were added to adjust the pH to 4.5. After stirring and reacting for 3 hours, the precipitate was separated by centrifugation. The precipitate was washed twice with anhydrous ethanol and then dispersed again in dimethyl sulfoxide. After being ultrasonically dispersed until evenly dispersed, 0.05 parts of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and 0.5 parts of 2,5-thiophene dicarboxylic acid were added. The mixture was heated to 95°C and stirred for 3 hours. After centrifugation, the precipitate was separated and dried to constant weight to obtain thiophene-modified attapulgite clay. B4. By weight, 10 parts of thiophene-modified attapulgite clay were dispersed in a 1.5 wt% acetic acid aqueous solution. After ultrasonic dispersion, 5 parts of chitosan oligosaccharide and 0.06 parts of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide were added. The mixture was heated to 85°C and stirred for 24 hours. The precipitate was then separated by centrifugation and redispersed in deionized water. Acetic acid was added to adjust the pH to 5. 0.05 parts of ferrous sulfate, 15 parts of hydrogen peroxide, and 0.1 parts of ascorbic acid were added. The mixture was heated to 42°C and stirred for 1 hour. 0.3 parts of naringenin were added. The mixture was kept at this temperature for 12 hours under a nitrogen atmosphere. The precipitate was then separated by centrifugation to obtain the antifungal and antibacterial agent.

[0017] Example 2. A method for preparing a compound peptide nutritional premix feed that reduces fat deposition, comprising the following steps: Compared with Example 1, this example increases the content of the complex peptide component, while the remaining steps remain unchanged; By weight, 60 parts of compound peptide, 20 parts of anti-mold and antibacterial agent and 800 parts of purified water are mixed and dispersed by ultrasonic vibration. After being mixed evenly, 50 parts of probiotic microcapsules and 0.2 parts of compound vitamins are added and stirred evenly. After being packaged, compound peptide nutritional premixed feed that reduces fat deposition is obtained. The complex peptide is composed of 30 parts bovine lactoferrin peptide, 12 parts glutathione, 12 parts soybean protein peptide and 6 parts casein peptide. The complex vitamin consists of 0.05 parts vitamin B1, 0.1 parts vitamin B2, 0.05 parts vitamin B6, and 0.03 parts vitamin B1. 12 composition.

[0018] Example 3. A method for preparing a compound peptide nutritional premix feed that reduces fat deposition, comprising the following steps: Compared with Example 2, this example increases the content of probiotic components, while the remaining steps remain unchanged; By weight, 60 parts of compound peptide, 20 parts of anti-mold and antibacterial agent and 800 parts of purified water are mixed and ultrasonically dispersed and mixed evenly. Then, 150 parts of probiotic microcapsules and 0.2 parts of compound vitamins are added and stirred evenly. After mixing, the mixture is packaged to obtain compound peptide nutritional premixed feed that reduces fat deposition. The complex peptide is composed of 30 parts bovine lactoferrin peptide, 12 parts glutathione, 12 parts soybean protein peptide and 6 parts casein peptide. The complex vitamin consists of 0.05 parts vitamin B1, 0.1 parts vitamin B2, 0.05 parts vitamin B6, and 0.03 parts vitamin B1. 12 Composition. Example 4. A method for preparing a compound peptide nutritional premixed feed that reduces fat deposition, comprising the following steps: Compared with Example 3, this example increases the content of the antifungal and antibacterial agent component, while the remaining steps remain unchanged; By weight, 60 parts of compound peptide, 30 parts of anti-mold and antibacterial agent and 800 parts of purified water are mixed and dispersed by ultrasonic vibration. After being mixed evenly, 150 parts of probiotic microcapsules and 0.2 parts of compound vitamins are added and stirred evenly. After being packaged, compound peptide nutritional premixed feed that reduces fat deposition is obtained. The complex peptide is composed of 30 parts bovine lactoferrin peptide, 12 parts glutathione, 12 parts soybean protein peptide and 6 parts casein peptide. The complex vitamin consists of 0.05 parts vitamin B1, 0.1 parts vitamin B2, 0.05 parts vitamin B6, and 0.03 parts vitamin B1. 12 composition.

[0019] Example 5. A method for preparing a compound peptide nutritional premixed feed that reduces fat deposition, comprising the following steps: Compared with Example 4, this example increases the amount of 2,5-thiophene dicarboxylic acid added in step B3, while the other steps remain unchanged; By weight, 60 parts of compound peptide, 30 parts of anti-mold and antibacterial agent and 800 parts of purified water are mixed and dispersed by ultrasonic vibration. After being mixed evenly, 150 parts of probiotic microcapsules and 0.2 parts of compound vitamins are added and stirred evenly. After being packaged, compound peptide nutritional premixed feed that reduces fat deposition is obtained. The complex peptide is composed of 30 parts bovine lactoferrin peptide, 12 parts glutathione, 12 parts soybean protein peptide and 6 parts casein peptide. The complex vitamin consists of 0.05 parts vitamin B1, 0.1 parts vitamin B2, 0.05 parts vitamin B6, and 0.03 parts vitamin B1. 12 composition; The preparation method of the antifungal and antibacterial agent includes the following steps: B1. After heating the attapulgite clay to 350℃ and calcining it for 1 hour, the water of crystallization and organic matter in it are removed to obtain calcined attapulgite clay. B2. Calcined attapulgite clay was dispersed in a 0.5 mol / L sulfuric acid solution. After stirring and reacting for 2 hours at room temperature, the precipitate was separated by centrifugation, washed with deionized water until the washing solution was neutral, and then dried to constant weight to obtain activated attapulgite clay. B3. By weight, 10 parts of activated attapulgite clay and 50 parts of anhydrous ethanol were mixed and stirred until evenly dispersed. Then, 5 parts of KH550 and 10 parts of deionized water were added, and the pH was adjusted to 4.5. After stirring and reacting for 3 hours, the precipitate was separated by centrifugation. The precipitate was washed twice with anhydrous ethanol and then dispersed again in dimethyl sulfoxide. After being ultrasonically dispersed until evenly dispersed, 0.05 parts of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and 3 parts of 2,5-thiophene dicarboxylic acid were added. The mixture was heated to 95°C and stirred for 3 hours. After centrifugation and separation of the precipitate, the mixture was dried to constant weight to obtain thiophene-modified attapulgite clay. B4. By weight, 10 parts of thiophene-modified attapulgite clay were dispersed in a 1.5 wt% acetic acid aqueous solution. After ultrasonic dispersion, 5 parts of chitosan oligosaccharide and 0.06 parts of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide were added. The mixture was heated to 85°C and stirred for 24 hours. The precipitate was then separated by centrifugation and redispersed in deionized water. Acetic acid was added to adjust the pH to 5. 0.05 parts of ferrous sulfate, 15 parts of hydrogen peroxide, and 0.1 parts of ascorbic acid were added. The mixture was heated to 42°C and stirred for 1 hour. 0.3 parts of naringenin were added. The mixture was kept at this temperature for 12 hours under a nitrogen atmosphere. The precipitate was then separated by centrifugation to obtain the antifungal and antibacterial agent.

[0020] Example 6. A method for preparing a compound peptide nutritional premix feed that reduces fat deposition, comprising the following steps: Compared with Example 5, this example increases the amount of chitosan oligosaccharide added in step B4, while the other steps remain unchanged; By weight, 60 parts of compound peptide, 30 parts of anti-mold and antibacterial agent and 800 parts of purified water are mixed and dispersed by ultrasonic vibration. After being mixed evenly, 150 parts of probiotic microcapsules and 0.2 parts of compound vitamins are added and stirred evenly. After being packaged, compound peptide nutritional premixed feed that reduces fat deposition is obtained. The complex peptide is composed of 30 parts bovine lactoferrin peptide, 12 parts glutathione, 12 parts soybean protein peptide and 6 parts casein peptide. The complex vitamin consists of 0.05 parts vitamin B1, 0.1 parts vitamin B2, 0.05 parts vitamin B6, and 0.03 parts vitamin B1. 12 composition; The preparation method of the antifungal and antibacterial agent includes the following steps: B1. After heating the attapulgite clay to 350℃ and calcining it for 1 hour, the water of crystallization and organic matter in it are removed to obtain calcined attapulgite clay. B2. Calcined attapulgite clay was dispersed in a 0.5 mol / L sulfuric acid solution. After stirring and reacting for 2 hours at room temperature, the precipitate was separated by centrifugation, washed with deionized water until the washing solution was neutral, and then dried to constant weight to obtain activated attapulgite clay. B3. By weight, 10 parts of activated attapulgite clay and 50 parts of anhydrous ethanol were mixed and stirred until evenly dispersed. Then, 5 parts of KH550 and 10 parts of deionized water were added, and the pH was adjusted to 4.5. After stirring and reacting for 3 hours, the precipitate was separated by centrifugation. The precipitate was washed twice with anhydrous ethanol and then dispersed again in dimethyl sulfoxide. After being ultrasonically dispersed until evenly dispersed, 0.05 parts of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and 3 parts of 2,5-thiophene dicarboxylic acid were added. The mixture was heated to 95°C and stirred for 3 hours. After centrifugation and separation of the precipitate, the mixture was dried to constant weight to obtain thiophene-modified attapulgite clay. B4. By weight, 10 parts of thiophene-modified attapulgite clay were dispersed in a 1.5 wt% acetic acid aqueous solution. After ultrasonic dispersion, 20 parts of chitosan oligosaccharide and 0.06 parts of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide were added. The mixture was heated to 85°C and stirred for 24 hours. The precipitate was then separated by centrifugation and redispersed in deionized water. Acetic acid was added to adjust the pH to 5. 0.05 parts of ferrous sulfate, 15 parts of hydrogen peroxide, and 0.1 parts of ascorbic acid were added. The mixture was heated to 42°C and stirred for 1 hour. 0.3 parts of naringenin were added. The mixture was kept at this temperature for 12 hours under a nitrogen atmosphere. The precipitate was then separated by centrifugation to obtain the antifungal and antibacterial agent.

[0021] Example 7. A method for preparing a compound peptide nutritional premixed feed that reduces fat deposition, comprising the following steps: Compared with Example 6, this example increases the amount of naringenin added in step B4, while the other steps remain unchanged; By weight, 60 parts of compound peptide, 30 parts of anti-mold and antibacterial agent and 800 parts of purified water are mixed and dispersed by ultrasonic vibration. After being mixed evenly, 150 parts of probiotic microcapsules and 0.2 parts of compound vitamins are added and stirred evenly. After being packaged, compound peptide nutritional premixed feed that reduces fat deposition is obtained. The complex peptide is composed of 30 parts bovine lactoferrin peptide, 12 parts glutathione, 12 parts soybean protein peptide and 6 parts casein peptide. The complex vitamin consists of 0.05 parts vitamin B1, 0.1 parts vitamin B2, 0.05 parts vitamin B6, and 0.03 parts vitamin B1. 12 composition; The preparation method of the antifungal and antibacterial agent includes the following steps: B1. After heating the attapulgite clay to 350℃ and calcining it for 1 hour, the water of crystallization and organic matter in it are removed to obtain calcined attapulgite clay. B2. Calcined attapulgite clay was dispersed in a 0.5 mol / L sulfuric acid solution. After stirring and reacting for 2 hours at room temperature, the precipitate was separated by centrifugation, washed with deionized water until the washing solution was neutral, and then dried to constant weight to obtain activated attapulgite clay. B3. By weight, 10 parts of activated attapulgite clay and 50 parts of anhydrous ethanol were mixed and stirred until evenly dispersed. Then, 5 parts of KH550 and 10 parts of deionized water were added, and the pH was adjusted to 4.5. After stirring and reacting for 3 hours, the precipitate was separated by centrifugation. The precipitate was washed twice with anhydrous ethanol and then dispersed again in dimethyl sulfoxide. After being ultrasonically dispersed until evenly dispersed, 0.05 parts of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and 3 parts of 2,5-thiophene dicarboxylic acid were added. The mixture was heated to 95°C and stirred for 3 hours. After centrifugation and separation of the precipitate, the mixture was dried to constant weight to obtain thiophene-modified attapulgite clay. B4. By weight, 10 parts of thiophene-modified attapulgite clay were dispersed in a 1.5 wt% acetic acid aqueous solution. After ultrasonic dispersion, 20 parts of chitosan oligosaccharide and 0.06 parts of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide were added. The mixture was heated to 85°C and stirred for 24 hours. The precipitate was then separated by centrifugation and redispersed in deionized water. Acetic acid was added to adjust the pH to 5. 0.05 parts of ferrous sulfate, 15 parts of hydrogen peroxide, and 0.1 parts of ascorbic acid were added. The mixture was heated to 42°C and stirred for 1 hour. Then, 3 parts of naringenin were added. The mixture was kept at this temperature for 12 hours under a nitrogen atmosphere. The precipitate was then separated by centrifugation to obtain the antifungal and antibacterial agent.

[0022] Comparative Example 1. A method for preparing a compound peptide nutritional premix feed that reduces fat deposition, comprising the following steps: Compared with Example 1, this comparative example did not prepare probiotic microcapsules, but only added lactic acid bacteria sludge, and the other steps remained unchanged; By weight, 10 parts of compound peptide, 20 parts of anti-mold and antibacterial agent and 800 parts of purified water are mixed and dispersed by ultrasonic vibration. After being mixed evenly, 50 parts of probiotic mud and 0.2 parts of compound vitamins are added and stirred evenly. After being packaged, compound peptide nutritional premixed feed that reduces fat deposition is obtained. The complex peptide is composed of 5 parts bovine lactoferrin peptide, 2 parts glutathione, 2 parts soybean protein peptide and 1 part casein peptide. The complex vitamin consists of 0.05 parts vitamin B1, 0.1 parts vitamin B2, 0.05 parts vitamin B6, and 0.03 parts vitamin B1. 12 composition; The method for preparing the probiotic sludge includes the following steps: A1. Mix skim milk with distilled water to prepare a skim milk solution with a concentration of 11wt%. Heat to boiling and keep warm for 15 minutes to sterilize. Then cool to 37±0.5℃ and inoculate with lactic acid bacteria. After the skim milk solidifies, continue to passage 3 times. Then cool to 4℃ and centrifuge to obtain probiotic sludge.

[0023] Comparative Example 2. A method for preparing a compound peptide nutritional premixed feed that reduces fat deposition, comprising the following steps: Compared with Example 1, no ascorbic acid was added in step B4 of this comparative example; By weight, 10 parts of compound peptide, 20 parts of anti-mold and antibacterial agent and 800 parts of purified water are mixed and dispersed by ultrasonic vibration. After being mixed evenly, 50 parts of probiotic microcapsules and 0.2 parts of compound vitamins are added and stirred evenly. After being packaged, compound peptide nutritional premixed feed that reduces fat deposition is obtained. The complex peptide is composed of 5 parts bovine lactoferrin peptide, 2 parts glutathione, 2 parts soybean protein peptide and 1 part casein peptide. The complex vitamin consists of 0.05 parts vitamin B1, 0.1 parts vitamin B2, 0.05 parts vitamin B6, and 0.03 parts vitamin B1. 12 composition; The preparation method of the probiotic microcapsules includes the following steps: A1. Skim milk and distilled water were mixed to prepare a skim milk with a concentration of 11wt%. The mixture was heated to boiling and kept at that temperature for 15 minutes for sterilization. After cooling to 37±0.5℃, lactic acid bacteria were inoculated into it. After the skim milk coagulated, it was passaged 3 times. After cooling to 4℃, it was centrifuged to obtain bacterial sludge. A2. Add sodium alginate to distilled water to prepare a sodium alginate aqueous solution with a concentration of 1.5 wt%. Mix 60 parts of sodium alginate aqueous solution with 5 parts of bacterial sludge by weight, add it to 60 parts of salad oil, add 0.15 parts of Tween 80, continue stirring and emulsifying for 30 minutes, then add 60 parts of calcium chloride aqueous solution dropwise. After the addition is completed, let it stand for 90 minutes, centrifuge to separate the precipitate, and wash it twice with deionized water to obtain probiotic microcapsules. The preparation method of the antifungal and antibacterial agent includes the following steps: B1. After heating the attapulgite clay to 350℃ and calcining it for 1 hour, the water of crystallization and organic matter in it are removed to obtain calcined attapulgite clay. B2. Calcined attapulgite clay was dispersed in a 0.5 mol / L sulfuric acid solution. After stirring and reacting for 2 hours at room temperature, the precipitate was separated by centrifugation, washed with deionized water until the washing solution was neutral, and then dried to constant weight to obtain activated attapulgite clay. B3. By weight, 10 parts of activated attapulgite clay and 50 parts of anhydrous ethanol were mixed and stirred until evenly dispersed. Then, 5 parts of KH550 and 10 parts of deionized water were added to adjust the pH to 4.5. After stirring and reacting for 3 hours, the precipitate was separated by centrifugation. The precipitate was washed twice with anhydrous ethanol and then dispersed again in dimethyl sulfoxide. After being ultrasonically dispersed until evenly dispersed, 0.05 parts of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and 0.5 parts of 2,5-thiophene dicarboxylic acid were added. The mixture was heated to 95°C and stirred for 3 hours. After centrifugation, the precipitate was separated and dried to constant weight to obtain thiophene-modified attapulgite clay. B4. By weight, 10 parts of thiophene-modified attapulgite clay were dispersed in a 1.5 wt% acetic acid aqueous solution. After ultrasonic dispersion, 5 parts of chitosan oligosaccharide and 0.06 parts of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide were added. The mixture was heated to 85°C and stirred for 24 hours. The precipitate was then separated by centrifugation and redispersed in deionized water. Acetic acid was added to adjust the pH to 5. 0.05 parts of ferrous sulfate and 15 parts of hydrogen peroxide were added. The mixture was heated to 42°C and stirred for 1 hour. 0.3 parts of naringenin were added. The mixture was kept at this temperature for 12 hours under a nitrogen atmosphere. The precipitate was then separated by centrifugation to obtain the antifungal and antibacterial agent.

[0024] The compound peptide nutritional premixed feed for reducing fat deposition prepared in Examples 1 to 7 and Comparative Examples 1 to 2 of this application was diluted with purified water at a ratio of 1:25. The diluted solution was then mixed with the feed at a mass ratio of 1:2 to obtain the test feed. Another ratio 3 was set up, and the comparison ratio 3 was used to test the feed mixed with only water; The feed consists of 55 parts corn, 20 parts wheat, 10 parts wheat bran, 5 parts soybean meal, 1 part cottonseed meal and 1.5 parts corn gluten meal. Cherry Valley ducks were used as the test group, with 100 ducks in each group. They were fed the test feed prepared in Examples 1-7 and Comparative Examples 1-2 respectively. The feeding and observation period was 28 days. During this period, the ducks were raised on wire mesh, with free access to feed and water. The test feed was changed every 24 hours, and they were exposed to continuous light for 24 hours. Ventilation and disinfection were carried out according to conventional methods. After the experimental period, their average daily weight gain, subcutaneous fat thickness and diarrhea rate were tested. The test results are shown in the table below.

[0025] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A compound peptide premixed feed for reducing fat deposition, characterized in that, By weight, the compound peptide nutritional premixed feed for reducing fat deposition consists of 0.23-1.5 parts compound vitamins, 10-60 parts compound peptides, 50-150 parts probiotic microcapsules, 20-30 parts anti-mold and antibacterial agent, and 800-1000 parts purified water.

2. The compound peptide nutritional premixed feed for reducing fat deposition according to claim 1, characterized in that: The complex vitamins, by weight, consist of 0.05-0.2 parts vitamin B1, 0.1-1 part vitamin B2, 0.05-0.2 parts vitamin B6, and 0.03-0.1 parts vitamin B1. 12 composition.

3. The compound peptide premixed feed for reducing fat deposition according to claim 1, characterized in that: The complex peptide, by weight, consists of 5-30 parts bovine lactoferrin peptide, 2-12 parts glutathione, 2-12 parts soybean protein peptide, and 1-6 parts casein peptide.

4. The compound peptide nutritional premixed feed for reducing fat deposition according to claim 1, characterized in that: The preparation method of the probiotic microcapsules includes the following steps: A1. Mix skim milk with distilled water, heat to boiling for sterilization, cool to 37±0.5℃, inoculate with probiotics, and after the skim milk coagulates, continue subculturing 2-3 times. Then cool to 2-4℃, centrifuge to separate the bacteria and obtain bacterial sludge. A2. Add sodium alginate to distilled water to prepare sodium alginate aqueous solution. Mix it evenly with bacterial sludge and add it to salad oil. Add Tween 80 and continue stirring and emulsifying for 10-30 minutes. Then, add calcium chloride aqueous solution dropwise. After the addition is complete, let it stand for 30-90 minutes. Centrifuge to separate the precipitate and wash it 2-3 times with deionized water to obtain probiotic microcapsules.

5. The compound peptide nutritional premixed feed for reducing fat deposition according to claim 4, characterized in that: In step A1, the probiotics are lactic acid bacteria.

6. The compound peptide premixed feed for reducing fat deposition according to claim 4, characterized in that: In step A2, by weight, the amounts of each component added are 60-85 parts sodium alginate aqueous solution, 5-20 parts bacterial mud, 60-80 parts salad oil, 0.15-0.3 parts Tween 80 and 60-80 parts calcium chloride aqueous solution. The sodium alginate aqueous solution has a sodium alginate concentration of 1.5-2 wt%, and the calcium chloride aqueous solution has a calcium chloride concentration of 2-4 wt%.

7. The compound peptide nutritional premixed feed for reducing fat deposition according to claim 1, characterized in that: The preparation method of the antifungal and antibacterial agent includes the following steps: B1. After heating the attapulgite clay to 350~380℃ and calcining it for 1~2 hours, the water of crystallization and organic matter are removed to obtain calcined attapulgite clay. B2. Disperse calcined attapulgite clay in a 0.5-2 mol / L sulfuric acid solution, stir and react for 2-4 hours at room temperature, centrifuge to separate the precipitate, wash with deionized water until the washing solution is neutral, and dry to constant weight to obtain activated attapulgite clay. B3. Mix activated attapulgite clay with anhydrous ethanol, stir and disperse evenly, add KH550 and deionized water, adjust the pH to 4-5, stir and react for 2-6 hours, centrifuge to separate the precipitate, wash with anhydrous ethanol 2-3 times, disperse the precipitate again in dimethyl sulfoxide, sonicate to disperse evenly, add 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and 2,5-thiophene dicarboxylic acid, heat to 85-105℃, stir and react for 2-4 hours, centrifuge to separate the precipitate, dry to constant weight to obtain thiophene-modified attapulgite clay; B4. Thiophene-modified attapulgite clay is dispersed in an aqueous acetic acid solution with a concentration of 1-2 wt%. After ultrasonic dispersion, chitosan oligosaccharide and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide are added. The mixture is heated to 75-85℃ and stirred for 12-24 hours. The precipitate is then separated by centrifugation and redispersed in deionized water. Acetic acid is added to adjust the pH to 4.5-5.

5. Ferrous sulfate, hydrogen peroxide, and ascorbic acid are added. The mixture is heated to 40-42℃ and stirred for 0.5-1 hour. Naringin is then added. The mixture is kept at this temperature under a nitrogen atmosphere for 12-24 hours. The precipitate is then separated by centrifugation to obtain an antifungal and antibacterial agent.

8. The compound peptide premixed feed for reducing fat deposition according to claim 7, characterized in that: In step B3, the amounts of each component added, by weight, are 10 parts activated attapulgite clay, 50-100 parts anhydrous ethanol, 5-15 parts KH550, 10-20 parts deionized water, 0.03-0.05 parts 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and 0.5-3 parts 2,5-thiophene dicarboxylic acid.

9. The compound peptide nutritional premixed feed for reducing fat deposition according to claim 7, characterized in that: In step B4, the amounts of each component added, by weight, are as follows: 10 parts thiophene-modified attapulgite clay, 5-20 parts chitosan oligosaccharide, 0.03-0.06 parts 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, 0.05-0.15 parts ferrous sulfate, 15-30 parts hydrogen peroxide, 0.1-0.3 parts ascorbic acid, and 0.3-3 parts naringenin.

10. A method for preparing a compound peptide nutritional premixed feed for reducing fat deposition as described in any one of claims 1 to 9, characterized in that, Includes the following steps: The compound peptides, antifungal and antibacterial agents, and purified water are mixed and dispersed by ultrasonic vibration. Probiotic microcapsules and compound vitamins are then added and stirred until evenly mixed. The mixture is then packaged to obtain a compound peptide nutritional premixed feed that reduces fat deposition.

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