A blood protein oligopeptide solid beverage, a preparation method and application thereof

Abstract

The application belongs to the technical field of food, and particularly relates to a blood protein oligopeptide solid beverage and a preparation method and application thereof. The blood protein oligopeptide solid beverage is mainly prepared from the following components by weight: 30-50 parts of blood protein oligopeptide; 15-25 parts of resistant dextrin and ferrous lactate complex; 20-30 parts of pH and enzyme double-response microsphere carrier; 8-12 parts of sea buckthorn fruit powder and vitamin C liposome; 15-25 parts of collagen peptide; and 0.5-1 part of silicon dioxide. The pH and enzyme double-response microsphere carrier is mainly composed of chitosan, pectin and trypsin cut polypeptide. The collagen peptide, blood protein oligopeptide and pH and enzyme double-response microsphere carrier are used to prepare microspheres. The blood protein oligopeptide is prepared by a fermentation method, and the pH and enzyme double-response microsphere carrier method is combined, so that the iron absorption rate and the stability of active ingredients are significantly improved.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of food technology, and particularly relates to a blood protein oligopeptide solid beverage, a preparation method thereof and application thereof. BACKGROUND

[0002] Solid beverage refers to a solid product with moisture not higher than 5 grams per 100 grams of finished product, in the form of powder, granules or blocks, such as bean crystal powder, milk powder, instant coffee, chrysanthemum crystal, etc., which is prepared by taking sugar, milk and dairy products, eggs or egg products, fruit juice or edible plant extracts as main raw materials and adding appropriate amounts of auxiliary materials or food additives.

[0003] Blood protein oligopeptide can improve iron deficiency anemia, increase hemoglobin, quickly provide nutrients required for the synthesis of blood cells, promote the synthesis of hemoglobin, platelets and white blood cells, has a protective effect on acute alcoholic liver damage and antioxidant function, and promotes the repair and regeneration of liver cells. It also has a protective effect on the damage of skeletal muscle and myocardium after exercise and has a significant anti-fatigue effect.

[0004] Collagen peptides can whiten the skin, remove spots and wrinkles, shrink pores, and moisturize, and can also promote bone formation and prevent osteoporosis. In addition, it also has the effects of protecting the gastric mucosa and preventing ulcers.

[0005] At present, the iron absorption rate of blood protein oligopeptide solid beverage is low, and traditional iron agents such as ferrous lactate and ferrous sulfate are easily oxidized to Fe 3+ in gastric acid with pH 1-2, which is difficult to be absorbed by the intestinal tract, and the absorption rate is usually ≤20%. Lack of targeted release: the existing technology directly mixes iron agents with peptide powder without intestinal directional release design, resulting in a large loss of iron in the stomach. Hemoglobin peptides are easily hydrolyzed in storage or in gastric acid environment, with a degradation rate of ≥20% in 6 months, resulting in loss of function. Traditional formula directly adds VC powder, which is rapidly oxidized in the presence of water and metal ions, and the retention rate is ≤30% in 3 months. SUMMARY

[0006] (I) Technical problems to be solved

[0007] In view of the above-mentioned shortcomings and deficiencies of the prior art, the present application provides a blood protein oligopeptide solid beverage, which is prepared by a fermentation method to prepare blood protein oligopeptide, and a pH and enzyme dual-responsive microsphere carrier method, which significantly improves the iron absorption rate and the stability of active ingredients.

[0008] Correspondingly, the present application also provides a preparation method of the blood protein oligopeptide solid beverage and application thereof in the preparation of products for improving iron deficiency anemia.

[0009] (ii) Technical solutions In order to achieve the above-mentioned purpose, the main technical solutions adopted by the present application include:

[0010] In a first aspect, the present application provides a blood protein oligopeptide solid beverage, which is mainly made of the following components by weight:

[0011] 30-50 parts of blood protein oligopeptide;

[0012] 15-25 parts of resistant dextrin and ferrous lactate complex;

[0013] 10-70 parts of pH and enzyme dual-responsive microsphere carrier;

[0014] 8-12 parts of sea buckthorn fruit powder and vitamin C liposome;

[0015] 15-25 parts of collagen peptide;

[0016] 0.5-1 part of silicon dioxide;

[0017] The pH and enzyme dual-responsive microsphere carrier is mainly composed of chitosan, pectin and trypsin cut polypeptide; the collagen peptide, blood protein oligopeptide and pH and enzyme dual-responsive microsphere carrier are used to make microspheres.

[0018] The resistant dextrin and ferrous lactate complex and the pH and enzyme dual-responsive microsphere carrier in the present application can double shield iron ions;

[0019] The blood protein peptide in the present application can first activate intestinal iron transport proteins, and then the released iron absorption efficiency is improved by 2 times. The liposome: the phospholipid bilayer isolates oxygen and metal ions, the release rate of VC in the stomach is ≤10%, and the release rate in the intestine is ≥90%; the positive charge of chitosan and the negative charge of the peptide are electrostatically adsorbed, reducing the contact with pepsin.

[0020] The sea buckthorn fruit powder containing organic acids and flavonoids in the present application can neutralize the metallic taste, and the mogroside can replace artificial sweeteners.

[0021] Alternatively, the preparation method of the blood protein oligopeptide includes the following steps: animal blood protein protease enzymolysis to obtain a blood protein hydrolysate, mixing carbon source and nitrogen source, inoculating plant lactobacillus and yeast fermentation, centrifugal separation to take supernatant, ultrafiltration to obtain blood protein oligopeptide liquid with a molecular weight of 500-1000 Da, and drying the obtained powder to obtain the blood protein oligopeptide.

[0022] More specifically, animal blood protein is added to protease for enzymolysis to obtain a blood protein hydrolysate, carbon source and nitrogen source are added and mixed, and the pH is adjusted to 6.0-7.0; plant lactobacillus and yeast are inoculated in the form of a mixed bacteria with an effective bacteria number ratio of 1:2-5, and the inoculation amount of the mixed bacteria is 1x10 6CFU / g; control fermentation conditions: temperature 35~38℃, time 48~72 hours, pH maintained at 5.5~6.5; after fermentation, the fermentation broth is inactivated at 60~65℃ for 15~20 minutes; after centrifugal separation, the supernatant is obtained, and blood protein oligopeptide liquid with a molecular weight of 500~1000 Da is obtained by ultrafiltration; the powder obtained by spray drying is the blood protein oligopeptide.

[0023] The market-purchased Lactobacillus plantarum has a preservation number of GDMCC NO.1.2868.

[0024] The market-purchased yeast has a preservation number of CICC 31796.

[0025] Optionally, the microspheres are mainly composed of an inner layer, an intermediate layer, and an outer layer from inside to outside.

[0026] The inner layer is mainly composed of collagen peptides and blood protein oligopeptides.

[0027] The intermediate layer is mainly composed of trypsin-digested polypeptides.

[0028] The outer layer is mainly composed of pectin and chitosan.

[0029] Optionally, the preparation method of the microspheres comprises the following steps: mixing blood protein oligopeptides and collagen peptides, dissolving them in deionized water, and drying to obtain peptide particles; dissolving the peptide particles in a trypsin-digested polypeptide solution, drying, adding to a chitosan solution to coat a chitosan layer, adjusting the pH, adding a pectin solution to coat a pectin layer as an outer layer, and drying to obtain the microspheres.

[0030] Optionally, the preparation method of the microspheres comprises the following steps: mixing blood protein oligopeptides and collagen peptides, dissolving them in deionized water, and drying to obtain peptide particles; dissolving the peptide particles in a trypsin-digested polypeptide solution, drying, adding to a chitosan solution with a pH of 5.0 to coat chitosan, adjusting the pH to 3.5, adding a pectin solution to coat a pectin layer as an outer layer, and drying to obtain the microspheres.

[0031] Optionally, the sequence of the trypsin-digested polypeptide is GPRPQ.

[0032] Optionally, the preparation method of the resistant dextrin and ferrous lactate complex comprises: ultrasonic treatment of ferrous lactate and resistant dextrin in a pH 4.5 phosphate buffer for 20~40 minutes, and then freeze-drying to form a resistant dextrin and ferrous lactate complex.

[0033] More specifically, the ferrous lactate and resistant dextrin are ultrasonically treated at 300~500W for 20~40 minutes in a pH 4.5 phosphate buffer, and then freeze-dried to form a porous carrier, which is a resistant dextrin and ferrous lactate complex.

[0034] Optionally, the preparation method of the sea buckthorn fruit powder and vitamin C liposome comprises the following steps: dissolving vitamin C, soybean lecithin and cholesterol in ethanol, evaporating into a film, and ultrasonic after hydration to obtain liposomes, and then mixing with sea buckthorn fruit powder to obtain the sea buckthorn fruit powder and vitamin C liposome.

[0035] More specifically, vitamin C, soybean lecithin and cholesterol are dissolved in ethanol at a weight ratio of 1:3-5:1-2, evaporated into a film by rotary evaporation, and ultrasonic after hydration to obtain liposomes, and then mixed with sea buckthorn fruit powder.

[0036] In a second aspect, the present application provides a preparation method of the blood protein oligopeptide solid beverage in any of the above-mentioned schemes, which comprises the following steps:

[0037] S1: preparing microspheres of collagen peptide, blood protein oligopeptide, pH and enzyme double-responsive microsphere carrier;

[0038] S2: dry mixing resistant dextrin and ferrous lactate complex, microspheres, sea buckthorn fruit powder and vitamin C liposome, adding nano-silicon dioxide, and homogenizing to obtain the blood protein oligopeptide solid beverage.

[0039] In a third aspect, the present application also provides the use of the blood protein oligopeptide solid beverage in any of the above-mentioned schemes in the preparation of an iron deficiency anemia improving product.

[0040] (Three) beneficial effects

[0041] The beneficial effects of the present application are:

[0042] The solid beverage in the present application can realize synergistic and efficient absorption of iron and peptides, solve the problem of low absorption rate, the active ingredients in the present application are embedded to improve stability, have zero stimulation to the stomach, and the taste is masked by a natural method. The iron absorption rate is increased to ≥50%,

[0043] Among them, the blood protein oligopeptide generated by fermentation forms a soluble complex with resistant dextrin to promote iron transcellular transport;

[0044] Among them, the microspheres release peptides to activate DMT1 in the intestinal tract, and the liposome VC reduces Fe 3+ to Fe 2+ , and the absorption efficiency is doubled. DETAILED DESCRIPTION

[0045] In order to explain the possible application scenarios, technical principles, specific schemes that can be implemented, purposes and effects that can be achieved, etc. of the present application, the following will be described in detail in combination with the specific embodiments listed. The embodiments described in this paper are only used to more clearly illustrate the technical solutions of the present application, and therefore only serve as examples, and cannot limit the protection scope of the present application.

[0046] Example 1

[0047] The embodiment provides a preparation method of blood protein oligopeptide, and steps are as follows:

[0048] S1, 10% (mass concentration) porcine hemoglobin solution is added into alkaline protease, the enzyme amount is 100 U / mL porcine hemoglobin solution, stirring hydrolysis is carried out at 50 DEG C for 4 hours, after enzyme inactivation, centrifugation is carried out to obtain supernatant;

[0049] S2, 2% (mass) glucose and 0.5% (mass) yeast extract are added into the supernatant, plant lactobacillus and yeast bacteria are inoculated, the effective bacteria number ratio is 1:4, the inoculation amount of the compound bacteria is 1x10 6 CFU / g porcine hemoglobin, pH is adjusted to 6.0, air is introduced at 0.205 VVM under micro-aerobic conditions, fermentation is carried out at 37 DEG C for 60 hours to obtain fermentation liquor, the fermentation liquor is purified by using a 1 kDa membrane ultrafiltration membrane to obtain an ultrafiltration liquor, then the ultrafiltration is carried out by using a 500 Da membrane to obtain blood protein oligopeptide material with a molecular weight cut-off of 500-1000 Da, and the product obtained by spray drying is the blood protein oligopeptide, the ferrihematin retention rate of which is 96.73%.

[0050] The plant lactobacillus and the yeast bacteria are purchased, the preservation number of the plant lactobacillus is GDMCC NO. 1.2868, and the preservation number of the yeast bacteria is CICC 31796.

[0051] Embodiment 2

[0052] The pH and enzyme double-response microsphere carrier is mainly composed of chitosan, pectin and trypsin cleavage polypeptide; the collagen peptide, the blood protein oligopeptide and the pH and enzyme double-response microsphere carrier are used to prepare microspheres.

[0053] The embodiment provides a preparation method of a solid beverage, and steps are as follows:

[0054] S1, the microspheres are prepared:

[0055] S11, 30 parts by weight of blood protein oligopeptide and 20 parts by weight of collagen peptide are dissolved in deionized water (total concentration 10% w / v, g / ml), and hydrochloric acid is used to adjust the pH to 7.0; spray drying is carried out, the inlet air temperature is 120 DEG C, the outlet air temperature is 60 DEG C, and peptide core microparticles with a particle size of 50-100 mu m are obtained;

[0056] S12, 7 parts by weight of trypsin cleavage polypeptide (the sequence is GPRPQ) is dissolved in pH 5.0 phosphate buffer (phosphate buffer) at a concentration of 2% w / v (g / ml), and then the peptide core microparticles are immersed in the solution, stirring is carried out at 25 DEG C for 1 hour, liquid is removed by centrifugal separation, fluidized bed drying is carried out at 45 DEG C, an intermediate layer coating is formed, and intermediate layer microparticles are prepared;

[0057] S13 chitosan solution with concentration of 1% w / v (g / ml) is prepared: chitosan is dissolved in acetic acid with concentration of 1% w / v (g / ml), and hydrochloric acid is added to adjust pH to 5.0;

[0058] Pectin solution with concentration of 1% w / v (g / ml) is prepared: pectin is dissolved in water to prepare the solution;

[0059] The intermediate layer microparticles are immersed in the chitosan solution, and the amount of chitosan added is 25% of the total mass of the microspheres. After stirring at 25°C for 1 hour, centrifugal washing is performed to remove the unabsorbed chitosan. The pH of the system is adjusted to 3.5, and the pectin solution is added. The amount of pectin added is 15% of the total mass of the microspheres, and stirring is performed for 1 hour. Centrifugal separation is performed, and freeze-drying is performed to obtain intact microspheres with a particle size of 80-150 μm;

[0060] S2 Preparation of resistant dextrin and ferrous lactate complex: ferrous lactate and resistant dextrin are mixed in a weight ratio of 1:8, and then phosphate buffer with pH 4.5 is added. The amount of phosphate buffer added is 40 times the mass of ferrous lactate. Ultrasonic treatment is performed at 400 W for 20 minutes, and then freeze-drying is performed to form a porous carrier, which is the resistant dextrin and ferrous lactate complex;

[0061] S3 Preparation of sea buckthorn powder and vitamin C liposomes: vitamin C, soy lecithin, and cholesterol are dissolved in ethanol in a weight ratio of 1:5:1.5. The mass concentration of soy lecithin in ethanol is 30%. Rotary evaporation is performed to form a film, and then hydration and ultrasonic treatment are performed to obtain liposomes with an average particle size of 200 nm. Then, the sea buckthorn powder and the vitamin C liposomes are mixed in a weight ratio of 1:1.

[0062] S4 Dry mixing of 25 parts by weight of resistant dextrin and ferrous lactate complex, all the obtained microspheres, 10 parts of sea buckthorn powder and vitamin C liposomes, and 0.5 parts of nano-silicon dioxide is performed, and then homogenization is performed to obtain a blood protein oligopeptide solid beverage.

[0063] Example 3

[0064] The present embodiment provides a preparation method of a solid beverage, and the steps are as follows:

[0065] S1 Preparation of microspheres:

[0066] S11 40 parts by weight of blood protein oligopeptide and 20 parts by weight of collagen peptide are dissolved in deionized water (concentration of 10% w / v, g / ml) to adjust the pH to 7.0. Spray drying is performed at an inlet temperature of 120°C and an outlet temperature of 60°C to obtain peptide core microparticles with a particle size of 50-100 μm.

[0067] S12 trypsin cut polypeptide (sequence GPRPQ) 10 parts by weight, dissolved in a phosphate buffer solution at pH 5.0 at a concentration of 2% (w / v, g / ml), then the peptide core microparticles were immersed in the solution, stirred at 25°C for 1 hour, centrifuged to remove the liquid, fluidized bed dried at 45°C to form an intermediate layer coating, and intermediate layer microparticles were prepared;

[0068] S13 chitosan solution with a concentration of 1% (w / v, g / ml) was prepared: chitosan was dissolved in acetic acid with a mass concentration of 1%, and the pH was adjusted to 5.0;

[0069] Pectin solution with a concentration of 1% (w / v, g / ml) was prepared: pectin was dissolved in water to prepare;

[0070] The intermediate layer microparticles were immersed in the chitosan solution, and the amount of chitosan added was 22% of the total mass of the microspheres. After stirring at 25°C for 1 hour, centrifugal washing was performed to remove the unabsorbed chitosan. Hydrochloric acid was used to adjust the pH of the system to 3.5, and pectin solution was added, with the amount of pectin added being 20% of the total mass of the microspheres. Stirring was performed for 1 hour. Centrifugal separation and freeze-drying were performed to obtain intact microspheres with a particle size of 80-150 μm.

[0071] S2 Preparation of resistant dextrin and ferrous lactate complex: Ferrous lactate and resistant dextrin were mixed at a weight ratio of 1:10 in a phosphate buffer solution at pH 4.5. The amount of phosphate buffer solution added was limited to 50 times the mass of ferrous lactate. Ultrasonic treatment was performed at 500W for 40 minutes, followed by freeze-drying to form a porous carrier of resistant dextrin and ferrous lactate complex.

[0072] S3 Preparation of sea buckthorn powder and vitamin C liposomes: More specifically, vitamin C, soy lecithin and cholesterol were dissolved in ethanol at a weight ratio of 1:3:2, with the mass concentration of soy lecithin in ethanol being 30%. Rotary evaporation was performed to form a film, and after hydration, ultrasonic treatment was performed to obtain liposomes with a particle size of 200 nm. Then, sea buckthorn powder and vitamin C were mixed at a weight ratio of 1:3.

[0073] S4 Dry mixing of 15 parts by weight of resistant dextrin and ferrous lactate complex, all the mass of microspheres obtained, sea buckthorn powder and vitamin C liposomes 12 parts, and adding 0.5 parts of nano-silicon dioxide, and homogenizing to obtain a blood protein oligopeptide solid beverage.

[0074] Example 4

[0075] The present embodiment provides a method for preparing a solid beverage, the steps of which are as follows:

[0076] S1 Preparation of microspheres:

[0077] S11 50 parts by weight of Sanguis Oligopeptide and 25 parts by weight of collagen peptide are dissolved in deionized water (concentration 10% w / v, g / ml), and hydrochloric acid is used to adjust the pH to 7.0; spray drying is performed with an inlet temperature of 120°C and an outlet temperature of 60°C to obtain peptide core microparticles with a particle size of 50-100 μm;

[0078] S12 10 parts by weight of trypsin-digested polypeptide (sequence GPRPQ) are stirred at a concentration of 2% (w / v, g / ml) at 25°C for 1 hour, and centrifugal separation is performed to remove the liquid, and fluidized bed drying is performed at 45°C to form an intermediate layer coating to obtain intermediate layer microparticles;

[0079] S13 A chitosan solution with a concentration of 1% (w / v, g / ml) is prepared: chitosan is dissolved in 1% acetic acid to form a solution, and the pH is adjusted to 5.0;

[0080] The intermediate layer microparticles are immersed in the chitosan solution, and the amount of chitosan added is 20% of the total mass of the microspheres, and stirring is performed at 25°C for 1 hour, and then centrifugal washing is performed to remove the unabsorbed chitosan; hydrochloric acid is used to adjust the pH of the system to 3.5, and a pectin solution is added, and the amount of pectin added is 18% of the total mass of the microspheres, and stirring is performed for 1 hour; centrifugal separation is performed, and freeze-drying is performed to obtain intact microspheres with a particle size of 80-150 μm;

[0081] S2 Preparation of resistant dextrin and ferrous lactate complex: after mixing ferrous lactate and resistant dextrin at a weight ratio of 1:15, a phosphate buffer solution is added at pH 4.5, and the amount of phosphate buffer solution added needs to be limited to 30 times the mass of ferrous lactate, and ultrasonic treatment is performed at 300W for 30 minutes, and then freeze-drying is performed to form a porous carrier which is a resistant dextrin and ferrous lactate complex.

[0082] S3 Preparation of sea buckthorn fruit powder and vitamin C liposomes: more specifically, vitamin C, soy lecithin and cholesterol are dissolved in ethanol at a weight ratio of 1:4:1, and the mass concentration of soy lecithin in ethanol is 30%, and rotary evaporation is performed to form a film, and after hydration, ultrasonic treatment is performed to obtain liposomes with a particle size of 200 nm, and then the liposomes are mixed with sea buckthorn fruit powder, and the weight ratio of sea buckthorn fruit powder to vitamin C is 1:2.

[0083] S4 After dry mixing 20 parts by weight of resistant dextrin and ferrous lactate complex, all the obtained microspheres, sea buckthorn fruit powder and vitamin C liposomes 8 parts, and adding 0.8 parts of nano-silicon dioxide, homogenization is performed to obtain a sanguis oligopeptide solid beverage.

[0084] Example 5

[0085] The present embodiment provides the use of the sanguis oligopeptide solid beverage in examples 2-4 in the preparation of a product for improving iron deficiency anemia.

[0086] In order to verify the unexpected technical effects of the embodiments of the present application, the following experiments were conducted.

[0087] 1. Iron absorption rate comparison experiment, the purpose is to verify the synergistic effect of double-response microspheres and blood protein oligopeptide.

[0088] Method: Caco-2 cells were seeded in Transwell chambers (pore size 0.4 μm), and cultured for 21 days to form a complete monolayer (TEER value ≥ 300 Ω·cm²). The iron transport amount (μg / mg protein) of Caco-2 cells was detected.

[0089] Place the sample to be tested (such as a solution) in a standard cuvette, and prepare a reference solution (usually pure solvent or blank matrix) to ensure that the optical paths of the two are consistent.

[0090] The measured sample absorbance A is directly used as the relative absorption rate value, or converted according to the formula: relative absorption rate (%) = (A / Amax) × 100%; Amax is the maximum absorbance of the sample under the same conditions, used for normalization to represent the absorption intensity.

[0091] Group A: solid beverage in Example 2 of the present application;

[0092] Group B: solid beverage prepared by replacing ferrous lactate with the same number of moles of commercially available ferrous sulfate according to the method of Example 2 of the present application;

[0093] Group C: solid beverage prepared by replacing microspheres with blood protein oligopeptide and trypsin-cleaved polypeptide according to the method of Example 2 of the present application without step S1;

[0094] Group D: solid beverage prepared by not adding ferrous lactate in step S2 according to the method of Example 2 of the present application;

[0095] Group E: solid beverage prepared by not adding blood protein oligopeptide in step S1 according to the method of Example 2 of the present application;

[0096] Group F: solid beverage prepared by replacing microspheres with trypsin-cleaved polypeptide according to the method of Example 2 of the present application without step S1.

[0097] Group G: solid beverage prepared by not adding collagen peptide in step S1 according to the method of Example 2 of the present application.

[0098] Table 1

[0099] Group Iron transport amount (μg / mg) Relative absorption rate (%) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 A 12.5 ± 1.2 100 B 4.8 ± 0.6 38.4 C 4.3 ± 0.2 34.4 D 2.1 ± 0.3 16.8 E 7.2 ± 0.9 57.6 F 4.6 ± 0.5 36.8 G 7.0 ± 0.8 56.0

[0100] From the data of table 1, it can be known that the iron absorption rate of group A is significantly higher than that of other groups (p<0.01), which indicates that the iron absorption rate of ferrous lactate used in the application is significantly higher than that of commercially available ferrous sulfate. The preparation of the microspheres of the application can also significantly improve the iron absorption rate.

[0101] From the comparison of the data of groups C and F, it can be known that when no microspheres are used, the addition of blood protein oligopeptide has no promoting effect on the relative absorption rate. From the comparison of the data of groups A and E, it can be known that under the premise of using microspheres, the addition of blood protein oligopeptide has a significant promoting effect on the relative absorption rate, which indicates that the microspheres and blood protein oligopeptide have a synergistic effect on improving the iron absorption rate.

[0102] In addition, from the comparison of the data of groups A, E and G, it can be known that the collagen peptide and blood protein oligopeptide have a high correlation and a synergistic effect on improving the iron transport amount and the relative absorption rate.

[0103] Finally, it should be pointed out that the above embodiments are only used to illustrate the technical solutions of the application, but not to limit the same; although the application has been described in detail with reference to the above embodiments, those skilled in the art should understand that they can still modify the technical solutions recorded in the above embodiments, or make equivalent replacement for part or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the application.

Claims

1. A hemoprotein oligopeptide solid beverage, characterized in that, It is mainly composed of the following components in parts by weight: 30-50 parts of hemoprotein oligopeptides; 15-25 parts of resistant dextrin and ferrous lactate complex; 10-70 portions of pH and enzyme dual-responsive microsphere carriers; 8-12 parts of sea buckthorn fruit powder and vitamin C liposomes; 15-25 parts collagen peptides; 0.5 to 1 part silica; The pH and enzyme dual-responsive microsphere carrier is composed of chitosan, pectin and trypsin-cleaved peptides; the collagen peptides, hemoprotein oligopeptides and pH and enzyme dual-responsive microsphere carrier are made into microspheres; The preparation method of the blood protein oligopeptide includes the following steps: enzymatic hydrolysis of animal blood protein with protease to obtain blood protein hydrolysate, adding carbon source and nitrogen source and mixing, inoculating with Lactobacillus plantarum and yeast for fermentation, centrifuging and separating, taking the supernatant, ultrafiltration to obtain blood protein oligopeptide liquid with molecular weight of 500~1000 Da, and drying to obtain the powder as the blood protein oligopeptide. The microspheres are composed of an inner layer, a middle layer, and an outer layer from the inside out; The inner layer is composed of collagen peptides and hemoprotein oligopeptides. The intermediate layer is composed of trypsin-cleaved polypeptides; The outer layer is composed of pectin and chitosan; The preparation method of the microspheres includes the following steps: mixing hemoprotein oligopeptides and collagen peptides, dissolving them in deionized water, and drying to obtain peptide particles; dissolving the peptide particles in a trypsin-digested polypeptide solution, drying them, adding them to a chitosan solution to coat them and form a chitosan layer, adjusting the pH and adding a pectin solution to coat them with a pectin layer as the outer layer, and drying them to obtain microspheres.

2. The hemoprotein oligopeptide solid beverage as described in claim 1, characterized in that: The sequence of the trypsin-cleaved polypeptide is GPRPQ.

3. The hemoprotein oligopeptide solid beverage as described in claim 1, characterized in that, The method for preparing the resistant dextrin and ferrous lactate complex includes: ultrasonically treating ferrous lactate and resistant dextrin in phosphate buffer and then freeze-drying to form the resistant dextrin and ferrous lactate complex.

4. The hemoprotein oligopeptide solid beverage as described in claim 1, characterized in that, The preparation method of the sea buckthorn fruit powder and vitamin C liposomes includes: dissolving vitamin C, soybean lecithin and cholesterol in ethanol, evaporating to form a film and hydrating it, then sonicating it to obtain liposomes, and then mixing it with sea buckthorn fruit powder to obtain the sea buckthorn fruit powder and vitamin C liposomes.

5. The method for preparing the hemoprotein oligopeptide solid beverage according to any one of claims 1-4, characterized in that, It includes the following steps: S1 forms microspheres from collagen peptides, hemoprotein oligopeptides, and pH and enzyme dual-responsive microsphere carriers. S2 involves dry mixing resistant dextrin and ferrous lactate complex, microspheres, sea buckthorn fruit powder, and vitamin C liposomes, then adding nano-silica and homogenizing to obtain a hemoprotein oligopeptide solid beverage.

6. The use of the hemoprotein oligopeptide solid beverage as described in any one of claims 1-4 in the preparation of products for improving iron deficiency anemia.