Composition for repairing skin barrier as well as preparation method and application thereof

Abstract

The invention provides a composition for repairing a skin barrier as well as a preparation method and application thereof, and relates to the technical field of cosmetics. The invention discloses a composition for repairing a skin barrier. The composition comprises ceramide NP1, ceramide AP, ceramide EOP, asiaticoside and DNA sodium. The composition is used for repairing skin from related proteins such as endogenous and endogenous injury, inflammatory factor inhibition, light injury, skin cuticle barrier and the like, and can deeply resist inflammation, relieve and maintain stability in an endogenous and endogenous two-way manner, and repair the damaged skin barrier in a multi-level and multi-dimensional manner, so that the skin problems such as skin dryness, roughness, desquamation, redness and swelling, pruritus and the like are improved.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and more specifically, to a composition for repairing the skin barrier and a method for preparing the same. Background Technology

[0002] The skin barrier is the outermost defense system of the skin, mainly composed of the stratum corneum (physical barrier) and the sebum film (chemical barrier). Its health directly affects the skin's appearance, sensitivity, moisturizing ability, and resistance to external stimuli.

[0003] The stratum corneum, located on the outermost layer of the skin, is the main component affecting barrier function. Keratinocytes, an important component of the stratum corneum, are formed by the division of epidermal stem cells located in the basal layer. During migration and differentiation, they synthesize and secrete another key component for repairing the stratum corneum—intercellular lipids. The two are layered together, with keratinocytes acting as "bricks" and intercellular lipids as "mortar," forming the "brick wall" barrier structure of the stratum corneum, providing a permeability barrier for the human body.

[0004] Keratinocytes, acting as the "bricks" of the skin barrier, have important barrier functions in both their cytoplasm and cell membrane. Intercellular lipids, also known as structural lipids, which form the "mortar" of this wall, are mainly composed of ceramides, free fatty acids, and cholesterol. Ceramides account for more than 50% of the composition and are the most important component of the "mortar."

[0005] The primary function of human skin is to establish an effective barrier, isolating the body from harmful external factors. A healthy skin barrier is a vital line of defense, protecting against allergens, harmful substances, and preventing moisture loss. Therefore, in a sense, a healthy skin barrier equates to beautiful, natural skin. However, under the stimulation of internal and external factors such as ultraviolet radiation, age, improper skincare, inferior skincare products and drug abuse, and various stresses, the skin barrier can be damaged, leading to insufficient self-defense capabilities and making the skin highly sensitive and prone to damage. Simultaneously, physical and chemical factors and microbial invasion can induce or exacerbate inflammatory responses, resulting in various skin diseases. Many skin diseases can lead to the destruction of the stratum corneum barrier function, and this barrier damage is itself a cause or aggravating factor for some skin diseases.

[0006] Existing research has shown that impaired skin barrier function not only causes or exacerbates inflammatory skin diseases, but also forms the basis for many common clinical skin conditions. Many skin-related problems are related to the skin barrier, such as sensitive skin, atopic dermatitis, chronic actinic dermatitis, psoriasis, and acne. Therefore, restoring the barrier function of the stratum corneum is crucial for maintaining skin health and alleviating and preventing skin diseases. Repairing the skin barrier is a key focus in the research and treatment of skin-related problems and a key consideration for major cosmetic companies in product development.

[0007] Currently, ceramides are commonly used as a key active ingredient in cosmetics for repairing the skin barrier function, primarily by increasing moisture in the stratum corneum to achieve barrier repair. However, due to difficulties in solubility in formulations, they are not widely used. Furthermore, this approach mainly focuses on repairing the physical barrier, and compositions used for this purpose are primarily based on mimicking the skin's natural lipid structure, mostly using ceramides as the core, combined with oils, moisturizers, and other ingredients. Their main functions are water retention, moisturizing, and soothing. While this approach can improve the skin barrier function to some extent, it cannot fundamentally address the problem of repairing the skin's structural structure. It also suffers from drawbacks such as poor stability, limited efficacy, single target, slow onset of action, short-lasting repair effects, and inability to maintain long-term barrier homeostasis. Summary of the Invention

[0008] The purpose of this invention is to provide a composition for repairing the skin barrier, which can repair the skin from the perspective of endogenous and exogenous damage, inhibition of inflammatory factors, photodamage, and related proteins of the skin stratum corneum barrier, thereby improving skin problems such as dryness, roughness, peeling, redness, swelling, and itching.

[0009] Another objective of this invention is to provide a method for preparing a composition for repairing the skin barrier, which is prepared into an emulsion through microemulsification, and can deeply reduce inflammation and soothe the skin, stabilize the skin from both internal and external sources, and repair the damaged skin barrier in a multi-level and multi-dimensional manner.

[0010] Another object of the present invention is to provide an application of a skin barrier repair composition in the preparation of cosmetics for repairing the skin barrier, which repairs the skin barrier while providing the skin with various nutrients required for barrier repair.

[0011] This invention is achieved through the following technical solution: In a first aspect, the present invention provides a composition for repairing the skin barrier, comprising ceramide NP1, ceramide AP, ceramide EOP, asiaticoside and sodium DNA.

[0012] Furthermore, the composition also includes 1,3-propanediol, butylene glycol, xanthan gum, Tween-80, and hydrogenated lecithin.

[0013] Furthermore, the composition comprises, by weight parts, the following components: 0.01-5 parts of ceramide NP1, 0.01-3 parts of ceramide AP, 0.001-1 parts of ceramide EOP, 0.01-1 parts of asiaticoside, 0.01-1 parts of sodium DNA, 10-50 parts of 1,3-propanediol, 5-20 parts of butylene glycol, 0.05-0.3 parts of xanthan gum, 0.5-5 parts of Tween-80, and 0.05-2 parts of hydrogenated lecithin.

[0014] Secondly, the present invention provides a method for preparing a composition for repairing the skin barrier, comprising the following steps: S1. Ceramide NP1, ceramide AP, ceramide EOP, asiaticoside and 1,3-propanediol are mixed and heated to obtain phase A; S2. Mix butanediol, xanthan gum, Tween-80, hydrogenated lecithin and purified water, heat and stir until completely dissolved to obtain phase B; S3. Add phase A to phase B, homogenize, and cool to obtain a mixed phase: S4. Add sodium DNA to the mixed phase, stir and mix to obtain the composition.

[0015] Furthermore, in S1 and S2, the temperature is heated to 80-85℃; in S3, the temperature is homogenized for 3-5 minutes at a rotation speed of 10000-30000 r / min and then cooled to 35-40℃.

[0016] Thirdly, the present invention provides the use of the above-mentioned skin barrier repair composition in the preparation of cosmetics for repairing the skin barrier.

[0017] Furthermore, the dosage forms of cosmetics include one or more of the following: aqueous solutions, lotions, serums, creams, ointments, powders, films, and gels.

[0018] Furthermore, the cosmetic product includes the above-described skin barrier repair composition and cosmetic-acceptable excipients.

[0019] Furthermore, the excipients include one or more of the following: emulsifiers, co-emulsifiers, skin conditioning agents, whitening agents, colorants, moisturizers, solubilizers, surfactants, preservatives, fragrances, skin moisturizers, anti-acne agents, film-forming agents, thickeners, pH adjusters, buffers, stabilizers, and ultraviolet absorbers.

[0020] Emulsifiers include one or more of glyceryl stearate, lecithin, polysorbate, cetearyl alcohol ether-20, and PEG-100 stearate. Co-emulsifiers include one or more of cholesterol, stearyl alcohol, beeswax, and sucrose stearate. Skin conditioning agents include one or more of sodium hyaluronate, panthenol, and squalane. Whitening agents include one or more of vitamin C derivatives, arbutin, kojic acid, dipotassium glycyrrhizate, and niacinamide. Coloring agents include one or more of iron oxide, titanium dioxide, chromium oxide green, and ultramarine. Moisturizers include one or more of glycerin, sorbitol, butylene glycol, and natural moisturizing factor (NMF). Solubilizers include one or more of PEG-40 hydrogenated castor oil, PPG-26-butanol polyether-26, octyl-dodecyl maltol, and PEG-6 caprylic / capric glyceride. Surfactants include one or more of sodium lauryl sulfate, cocamide DEA, lauryl glucoside, sodium cocoamphoacetate, and polyquaternium-7. Preservatives include one or more of phenoxyethanol, methylisothiazolinone, parabens, sodium benzoate, and glutaraldehyde. Fragrances include one or more of lavender essential oil, rosemary essential oil, vanillin, citronellol, and linalool. Emollients include one or more of squalane, mineral oil, isononyl isononanoate, sweet almond oil, and jojoba oil. Anti-acne agents include one or more of salicylic acid, benzoyl peroxide, nicotinamide, and tea tree oil. Film-forming agents include polyvinylpyrrolidone (PVP) or triethylhexyl triazine. Thickeners include one or more of carboxymethyl cellulose, xanthan gum, hydroxyethyl cellulose, carbomer, and gelatin. pH adjusters include one or more of citric acid, sodium hydroxide, potassium hydroxide, phosphoric acid, and L-arginine. Buffers include one or more of sodium lactate, phosphate, and sodium citrate. Stabilizers include one or more of disodium EDTA, butylated hydroxytoluene, ascorbate palmitate, tocopherol, and silicone oil. UV absorbers include one or more of butyl methoxydibenzoylmethane, ethylhexyl methoxycinnamate, and hexyl diethylaminohydroxybenzoylbenzyl benzoate.

[0021] This invention uses ceramides, asiaticoside, and sodium DNase as the main active ingredients in a composition. It rapidly repairs and stabilizes the skin barrier from multiple layers and dimensions, both internally and externally. It not only locks in moisture, moisturizes, and strengthens the "brick wall" structure of the stratum corneum, but also repairs cellular DNA damage caused by various factors such as ultraviolet radiation. It penetrates layer by layer, deeply repairs, provides deep anti-inflammatory and soothing effects, promotes skin cell regeneration, and rapidly repairs the skin barrier at multiple targets, maintaining long-term barrier homeostasis and fundamentally solving skin barrier problems. Experimental results show that this composition has a better skin barrier repair effect than ceramides alone. This composition can be used to prepare various cosmetics with skin barrier repair functions, including serums, lotions, freeze-dried powders, creams, masks, and lotions. It provides dual-source stabilization and multi-layered repair of the skin barrier, thereby improving skin problems such as roughness, flaking, itching, and dryness.

[0022] Various products based on the compositions described in this invention can be used on the skin of the face, eyes, neck, hands, feet, and the whole body. They can promote DNA repair, provide deep anti-inflammatory and soothing effects, promote skin cell regeneration, reduce photodamage and photoaging caused by ultraviolet rays, prevent skin moisture loss, increase the moisture content of the stratum corneum, rapidly repair the skin barrier through multiple targets, maintain the health and integrity of the skin barrier function, and maintain long-term barrier homeostasis.

[0023] The composition used in this invention is rich in ceramide NP1, ceramide AP, ceramide EOP, sodium DNA, asiaticoside, and other ingredients. It repairs the skin by addressing internal and external damage, inhibition of inflammatory factors, photodamage, and related proteins in the skin's stratum corneum barrier. It can work in multiple levels and dimensions, providing bidirectional stabilization, anti-allergy and soothing, and rapid repair of the damaged skin barrier, thereby improving skin problems such as dryness, roughness, peeling, redness, swelling, and itching.

[0024] The stratum corneum's brick-and-mortar structure is a crucial component of the skin barrier, and the barrier function of a normal epidermis depends on the quality of its "bricks" and "mortar." Ceramides are a major component of the stratum corneum's lipids, directly affecting the barrier's tightness and strength, playing a vital role in stratum corneum structural stability, signal transduction, cell proliferation and differentiation, epidermal barrier repair, and immune regulation. Currently, 12 different subtypes of ceramides have been identified in the human stratum corneum, including ceramide NP, NS, AS, AP, and EOP. Changes in both the quality and quantity of ceramides can alter the lipid structure of the stratum corneum, changing skin barrier function and further leading to related skin diseases. The moisturizing function of the stratum corneum is also closely related to ceramides. Ceramides are highly effective moisturizers; their structure contains numerous hydrophilic groups, exhibiting a strong affinity for water. They can promote epidermal hydration, reduce epidermal water evaporation, enhance epidermal cell cohesion, and prevent dry, flaky skin. Ceramides, as important lipids in the epidermis, possess multiple functions including moisturizing, adhesion, barrier function, immune regulation, anti-allergy, and anti-aging. In particular, as lipid second messengers, they play various roles such as regulating cell cycle and differentiation, and inducing apoptosis. They are key signaling molecules in intracellular oxidative stress pathways and participate in many pathophysiological processes. Abnormal ceramide levels can affect the skin barrier function. Factors influencing ceramide levels in the skin include age, climate, season, and excessive cleansing. Numerous studies have shown that in problem skin, or under the influence of seasonal factors, the levels of ceramides EOP and ceramide NP are reduced in the skin. Research indicates that supplementing with appropriate multi-ceramide complexes is a very direct way to repair the skin barrier. This method can significantly increase skin hydration, improve transepidermal water loss, and simultaneously improve skin texture, wrinkles, and redness.

[0025] Asiaticoside is produced from the whole plant of Centella asiatica through processes such as crushing, extraction, elution, and decolorization. Both asiaticoside and hydroxyasiaticoside are isolated from asiaticoside. Asiaticoside plays a unique role in both pharmaceuticals and cosmetics. It promotes wound healing, inhibits scar hyperplasia, and possesses various pharmacological effects including antibacterial, anti-inflammatory, and anti-tumor properties. It can be used to treat external injuries, surgical wounds, burns, scars, and scleroderma. Centella asiatica extract is also a popular ingredient in the cosmetics industry, exhibiting the following benefits: 1. Asiaticoside helps promote collagen formation in the dermis, improves the skin barrier, alleviates allergies, and keeps the skin healthy and stable, providing repair benefits. 2. Asiaticoside has significant anti-inflammatory effects, inhibiting inflammatory responses, reducing inflammatory symptoms, and helping to promote the repair and healing of skin wounds, providing anti-inflammatory and soothing effects. 3. Asiaticoside is rich in various natural antioxidants, which can neutralize free radicals, reduce oxidative stress, protect cells from oxidative damage, and help delay aging.

[0026] Sodium DNA, also known as PDRN, is a sodium salt form of natural DNA, typically obtained through extraction from biological tissues such as fish semen and animal tissues. It is a nucleic acid derivative containing abundant nucleotide fragments (such as adenine, guanine, cytosine, and thymine) and their associated phosphate backbone. Sodium DNA can act on multiple layers of the skin (epidermis, dermis, and extracellular matrix), achieving repair, anti-aging, and moisturizing functions through a series of biological mechanisms. The nucleotide fragments (such as guanine and adenine) provided by sodium DNA can penetrate into skin cells and participate in intracellular DNA repair mechanisms. This helps repair DNA strand breaks or mutations in skin cells caused by environmental stressors such as ultraviolet radiation and pollution, restoring gene integrity. The phosphate groups in sodium DNA can capture free radicals, reducing the accumulation of reactive oxygen species (ROS) and effectively resisting oxidative damage caused by ultraviolet radiation and pollution, thereby delaying skin aging. Sodium DNA can also regulate the release of skin inflammatory factors (such as IL-6 and TNF-α), alleviating inflammatory responses, making it particularly suitable for sensitive skin or post-operative repair. Sodium DNA forms a transparent film on the skin surface, reducing transepidermal water loss (TEWL) and thus locking in moisture. By repairing keratinocytes, sodium DNA enhances the integrity of the skin barrier, reducing the penetration of external irritants. In summary, sodium DNA has a wide range of applications in skincare products, covering multiple aspects such as repairing DNA damage, anti-oxidation, anti-inflammatory and soothing effects, moisturizing, and barrier repair, providing comprehensive protection for skin health.

[0027] The technical solution of the present invention has at least the following advantages and beneficial effects: 1. The composition of the present invention is rich in components. The composition uses ceramides, asiaticoside, and sodium DNase as the main active ingredients, and the ceramides include ceramide NP1, ceramide AP, and ceramide EOP. At the same time, it is treated with microemulsion technology, which makes the entire composition rich in components and easy to penetrate the skin. It can be used to repair the skin caused by exogenous damage, inflammation suppression, and the repair of related proteins such as the skin stratum corneum barrier, thereby improving skin problems such as roughness, peeling, redness, swelling, itching, and dryness.

[0028] 2. The composition described in this invention can rapidly repair the skin barrier at multiple levels and targeting multiple points, maintaining long-term barrier homeostasis and fundamentally solving skin barrier problems. This composition can directly replenish the lipids of the stratum corneum with various subtypes of ceramides, strengthening the stratum corneum's structural integrity and enhancing the skin barrier from the outside in. Short-chain ceramides can reach the active epidermis and dermis, exerting their effects in the deeper layers of the skin. The asiaticoside in the composition has anti-inflammatory and soothing properties, while sodium DNA can act on multiple layers of the skin (epidermis, dermis, and extracellular matrix), repairing DNA damage caused by various factors such as ultraviolet radiation, promoting skin cell regeneration from the inside out. In summary, the active ingredients in the composition, such as ceramides, asiaticoside, and sodium DNA, work synergistically to repair the skin barrier layer by layer, addressing multiple targets and rapidly repairing the skin barrier to maintain barrier homeostasis.

[0029] 3. Products such as serums, lotions, freeze-dried powders, creams, masks, lotions, and facial cleansers can be prepared using this composition. While providing the skin with various nutrients needed for barrier repair, it can also deeply reduce inflammation, soothe, stabilize the skin from both internal and external sources, and repair the skin barrier in multiple layers and dimensions, thereby improving skin problems such as roughness, peeling, itching, and dryness. Attached Figure Description

[0030] Figure 1 A flowchart for testing the efficacy of the composition for repairing the skin barrier provided by this invention; Figure 2 The images are microscopic images of the tissue morphology of experimental examples of the present invention, wherein the first row is group BC, the second row is group NC, the third row is group PC1, the fourth row is experimental group A, and the fifth row is experimental group B. Figure 3 This is a bar chart showing the content of sunburn cells in the tissue morphology of the experimental examples of the present invention; Figure 4 The image shows the fluorescence detection of LOR in the experimental example of this invention, wherein the first row is group BC, the second row is group NC, the third row is group PC1, the fourth row is experimental group A, and the fifth row is experimental group B. Figure 5 This is a bar chart showing the LOR content of the experimental examples of the present invention; Figure 6 The image shows the fluorescence detection of SPT1 in the experimental example of this invention, wherein the first row is group BC, the second row is group NC, the third row is group PC1, the fourth row is experimental group A, and the fifth row is experimental group B. Figure 7 This is a bar chart showing the SPT1 content in the experimental examples of the present invention. Figure 8The image shows the fluorescence detection of NF-κB in the experimental examples of this invention. The first row is group BC, the second row is group NC, the third row is group PC2, the fourth row is experimental group A, and the fifth row is experimental group B. Figure 9 This is a bar chart showing the NF-κB content in the experimental examples of this invention. Figure 10 The image shows the fluorescence detection of TNF-α in the experimental examples of this invention. The first row is group BC, the second row is group NC, the third row is group PC2, the fourth row is experimental group A, and the fifth row is experimental group B. Figure 11 This is a bar chart showing the TNF-α content in the experimental examples of this invention; Figure 12 The images are microscopic images of the CPD in the experimental examples of the present invention, wherein the first row is group BC, the second row is group NC, the third row is group PC3, the fourth row is experimental group A, and the fifth row is experimental group B. Figure 13 This is a bar chart showing the CPD content in the experimental examples of this invention. Detailed Implementation

[0031] The present invention will be further described below with reference to specific embodiments, but the embodiments do not limit the present invention in any way. Unless otherwise stated, the raw materials and reagents used in the embodiments of the present invention are conventionally purchased raw materials and reagents.

[0032] Example 1 This embodiment provides a method for preparing a composition for repairing the skin barrier, comprising the following steps: Phase A comprises the following raw materials in parts by weight: 0.01 parts ceramide NP1, 0.01 parts ceramide AP, 0.001 parts ceramide EOP, 0.01 parts asiaticoside, and 10 parts 1,3-propanediol; Phase B comprises the following raw materials in parts by weight: 5 parts butylene glycol, 0.05 parts xanthan gum, 0.5 parts Tween-80, 0.05 parts hydrogenated lecithin, and 40 parts purified water; Phase C comprises the following raw materials in parts by weight: 0.01 parts sodium DNA. S1. Mix the A-phase raw materials and heat to 80℃ to obtain the A-phase; S2. Mix the B phase raw materials and heat to 80°C, stirring until completely dissolved to obtain the B phase; S3. Add phase A to phase B, stir until homogenized, homogenize at 10000 r / min for 3 min, and cool to 35℃ to obtain the mixed phase: S4. Add the C phase raw material to the mixed phase, stir and mix to obtain the composition.

[0033] Example 2 This embodiment provides a method for preparing a composition for repairing the skin barrier, comprising the following steps: Phase A comprises the following raw materials in parts by weight: 5 parts ceramide NP1, 3 parts ceramide AP, 1 part ceramide EOP, 1 part asiaticoside, and 50 parts 1,3-propanediol; Phase B comprises the following raw materials in parts by weight: 20 parts butylene glycol, 0.3 parts xanthan gum, 5 parts Tween-80, 2 parts hydrogenated lecithin, and 70 parts purified water; Phase C comprises the following raw material in parts by weight: 1 part sodium DNA. S1. Mix the A-phase raw materials and heat to 85°C to obtain the A-phase; S2. Mix the B phase raw materials and heat to 85°C, stirring until completely dissolved to obtain the B phase; S3. Add phase A to phase B, stir until homogenized, homogenize at 30000 r / min for 5 min, and cool to 40℃ to obtain the mixed phase: S4. Add the C phase raw material to the mixed phase, stir and mix to obtain the composition.

[0034] Example 3 This embodiment provides a method for preparing a composition for repairing the skin barrier, comprising the following steps: Phase A comprises the following raw materials in parts by weight: 1 part ceramide NP1, 1 part ceramide AP, 0.5 part ceramide EOP, 0.5 part asiaticoside, and 30 parts 1,3-propanediol; Phase B comprises the following raw materials in parts by weight: 15 parts butylene glycol, 0.15 parts xanthan gum, 3 parts Tween-80, 1 part hydrogenated lecithin, and 50 parts purified water; Phase C comprises the following raw materials in parts by weight: 0.5 parts sodium DNA. S1. Mix the A-phase raw materials and heat to 82℃ to obtain the A-phase; S2. Mix the B phase raw materials and heat to 82°C, stirring until completely dissolved to obtain the B phase; S3. Add phase A to phase B, stir until homogeneous, homogenize at 20000 r / min for 4 min, and cool to 38℃ to obtain the mixed phase: S4. Add the C phase raw material to the mixed phase, stir and mix to obtain the composition.

[0035] Test case Since skin redness is clinically visible after UV irradiation, an in vitro 3D skin model was used as the research subject. An in vitro damage model was established using UVB irradiation. The soothing efficacy was analyzed by detecting changes in biological indicators after treatment with the active ingredient. The detection indicators included CPD (cold dermal lesions), tissue morphology (sunbrun cell), inflammatory signaling pathway protein NF-κB, inflammatory factor TNF-α, serine palmitoyltransferase (SPT1), and LOR (lower irritation level). The detection methods included immunohistochemistry (IHC), hematoxylin-eosin staining (HE), and immunofluorescence (IF).

[0036] The experimental groups are as follows: Table 1

[0037] Among them, WY14643 is a positive control for detecting tissue morphology, LOR, and SPT1; dexamethasone is a positive control for detecting NF-κB and TNF-α; and VE is a positive control for detecting CPD. Sample A is the composition prepared in Example 1, and sample B is a composition containing only ceramides.

[0038] When performing statistical analysis using the t-test method, significance compared to the BC group is indicated by #, with P-value < 0.05 indicated by # and P-value < 0.01 indicated by ##. Compared to the NC group, significance is indicated by *, with P-value < 0.05 indicated by * and P-value < 0.01 indicated by **.

[0039] 1. Results of tissue morphology testing Table 2

[0040] Figure 2 The images were taken using an upright microscope (Olympus, BX53) at a magnification of 400×, with the cell nuclei in blue.

[0041] Figure 3 Analysis showed that the number of Sunburn cells increased in the NC group compared to the BC group, indicating that the stimulation conditions were effective in this test. The number of Sunburn cells decreased in the PC1 group compared to the NC group, indicating that the positive control test was effective. Compared to the NC group, the number of Sunburn cells decreased in experimental groups A and B, with inhibition rates of 47.25% and 25.00%, respectively.

[0042] The results showed that after the test sample was used to model the disease, the sunburn cell count decreased significantly, indicating that the test sample had a repairing effect.

[0043] 2. LOR detection results Table 3

[0044] Figure 4 The images were taken using a fluorescence microscope (Olympus, BX43) at a magnification of 200×. Blue fluorescence represents cell nuclei, and green fluorescence represents LOR (Leakage Reduction). The stronger the green fluorescence, the higher the LOR content.

[0045] Figure 5 Analysis showed that the LOR content in the NC group was significantly lower than that in the BC group, indicating that the stimulation conditions in this test were effective. The LOR content in the PC1 group was significantly higher than that in the NC group, indicating that the positive control test was effective. Compared with the NC group, the LOR content in experimental groups A and B was significantly higher, with increases of 175.86% and 68.97%, respectively.

[0046] The results showed that the test sample increased the content of LOR protein after treatment, indicating that the test sample has the effect of repairing the skin barrier.

[0047] 3. SPT1 test results Table 4

[0048] Figure 6 The images were taken using a fluorescence microscope (Olympus, BX43) at a magnification of 200×. Blue fluorescence represents cell nuclei, and green fluorescence represents SPT1. The stronger the green fluorescence, the higher the SPT1 content.

[0049] Figure 7 Analysis showed that the SPT1 level in the NC group was significantly lower than that in the BC group, indicating that the stimulation conditions in this test were effective. The SPT1 level in the PC1 group was significantly higher than that in the NC group, indicating that the positive control test was effective. The SPT1 levels in experimental groups A and B were significantly higher than those in the NC group, with increases of 180.65% and 64.52%, respectively.

[0050] The results showed that SPT1 expression increased after treatment with the sample, indicating that the sample had a repairing effect.

[0051] 4. NF-κB detection results Table 5

[0052] Figure 8 The images were taken using a fluorescence microscope (Olympus, BX43) at a magnification of 200×. Blue fluorescence represents cell nuclei, and red fluorescence represents NF-κB. The stronger the red fluorescence, the higher the NF-κB content.

[0053] Figure 9 Analysis showed that the NF-κB content in the NC group was significantly higher than that in the BC group, indicating that the stimulation conditions in this test were effective. The NF-κB content in the PC2 group was significantly lower than that in the NC group, indicating that the positive control test was effective. Compared with the NC group, the NF-κB content in experimental groups A and B was significantly lower, with inhibition rates of 62.94% and 39.16%, respectively.

[0054] The results showed that after the test substance was applied to the model, the secretion of NF-κB decreased, indicating that the sample had a soothing effect by inhibiting the expression of inflammatory factors.

[0055] 5. TNF-α detection results Table 6

[0056] Figure 10 The images were taken using a fluorescence microscope (Olympus, BX43) at a magnification of 200×. Blue fluorescence represents cell nuclei, and green fluorescence represents TNF-α. The stronger the green fluorescence, the higher the TNF-α content.

[0057] Figure 11 Analysis showed that the TNF-α level in the NC group was significantly higher than that in the BC group, indicating that the stimulation conditions in this test were effective. The TNF-α level in the PC2 group was significantly lower than that in the NC group, indicating that the positive control test was effective. Compared with the NC group, the TNF-α levels in experimental groups A and B were significantly lower, with inhibition rates of 50.00% and 23.02%, respectively.

[0058] The results showed that the expression of TNF-α decreased after treatment with the test substance, indicating that it has a sunburn prevention effect.

[0059] 6. CPD test results Table 7

[0060] Figure 12 The images were taken using an upright microscope (Olympus, BX53) at a magnification of 400×. The brown color represents DNA damage cyclobutane pyrimidine dimers (CPDs). The lighter the brown color, the lower the content of DNA damage cyclobutane pyrimidine dimers (CPDs).

[0061] Figure 13Analysis showed that the CPD content in the NC group was significantly higher than that in the BC group, indicating that the stimulation conditions in this test were effective. The CPD content in the PC3 group was significantly lower than that in the NC group, indicating that the positive control test was effective. Compared with the NC group, the CPD content in experimental groups A and B was significantly lower, with inhibition rates of 25.32% and 8.90%, respectively.

[0062] The results showed that, compared with the NC group, the CPD positivity rate of the epidermis decreased after treatment with the test substance, indicating that the test substance has the effect of protecting the skin from damage caused by ultraviolet rays and has an antioxidant effect.

[0063] In summary, the composition prepared by ceramide, asiaticoside, and sodium DNase of the present invention exhibits higher inhibition rates against sunburn cells, NF-κB, TNF-α, and CPD, and higher enhancement rates against LOR and SPT1, compared to compositions prepared using only ceramide. This indicates that the composition of the present invention has better repair effects on the skin barrier and provides better soothing, sunburn protection, and antioxidant effects.

[0064] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A composition for repairing the skin barrier, characterized in that, It includes ceramide NP1, ceramide AP, ceramide EOP, asiaticoside, and sodium DNA.

2. The composition according to claim 1, characterized in that, The composition also includes 1,3-propanediol, butylene glycol, xanthan gum, Tween-80, and hydrogenated lecithin.

3. The composition according to claim 2, characterized in that, By mass, it includes the following components: 0.01-5 parts of ceramide NP1, 0.01-3 parts of ceramide AP, 0.001-1 parts of ceramide EOP, 0.01-1 parts of asiaticoside, 0.01-1 parts of sodium DNA, 10-50 parts of 1,3-propanediol, 5-20 parts of butylene glycol, 0.05-0.3 parts of xanthan gum, 0.5-5 parts of Tween-80, and 0.05-2 parts of hydrogenated lecithin.

4. The method for preparing the composition for repairing the skin barrier as described in claim 3, characterized in that, Includes the following steps: S1. Ceramide NP1, ceramide AP, ceramide EOP, asiaticoside and 1,3-propanediol are mixed and heated to obtain phase A; S2. Mix butanediol, xanthan gum, Tween-80, hydrogenated lecithin and purified water, heat and stir until completely dissolved to obtain phase B; S3. Add phase A to phase B, homogenize, and cool to obtain a mixed phase: S4. Add sodium DNA to the mixed phase, stir and mix to obtain the composition.

5. The method for preparing the composition for repairing the skin barrier according to claim 4, characterized in that: In S1 and S2, heat to 80-85℃; in S3, homogenize for 3-5 minutes at a speed of 10000-30000 r / min, and then cool to 35-40℃.

6. The use of the skin barrier repair composition according to any one of claims 1-3 in the preparation of cosmetics for repairing the skin barrier.

7. The application according to claim 6, characterized in that, The dosage forms of the cosmetics include one or more of the following: aqueous solutions, lotions, serums, creams, ointments, powders, films, and gels.

8. The application according to claim 6, characterized in that, The cosmetic comprises the skin barrier repair composition according to any one of claims 1-4 and cosmetically acceptable excipients.

9. The application according to claim 8, characterized in that, The excipients include one or more of the following: emulsifiers, co-emulsifiers, skin conditioning agents, whitening agents, colorants, moisturizers, solubilizers, surfactants, preservatives, fragrances, skin moisturizers, anti-acne agents, film-forming agents, thickeners, pH adjusters, buffers, stabilizers, and ultraviolet absorbers.

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