Preparation method of biological fertilizer for ecological restoration of soil

Abstract

The invention discloses a preparation method of a biological fertilizer for ecological restoration of soil. The preparation method comprises the following steps: step 1, collecting branches and leaves trimmed from shiny-leaved yellowhorn trees, shells removed when shiny-leaved yellowhorn seeds are collected and cakes formed by processing edible oil; 2, putting all the collected shiny-leaved yellowhorn wastes into a mixing and crushing machine for mixing and crushing; 3, natural infectious microbes in the crushed materials are eliminated in a steam disinfection mode; step 4, adding a combined fermentation inoculant into the disinfected crushed material; step 5, controlling the temperature and humidity of the fermentation product; step 6, fermenting the fermented product under a ventilated and breathable condition; 7, detecting the content of nutritional ingredients of the fermented feed; and 8, adjusting the proportion of N, P and K. The invention belongs to the technical field of soil remediation, particularly relates to a preparation method of a biological fertilizer for ecological remediation of soil, and aims to at least solve the problem that the improvement effect of a traditional microbial fertilizer on soil is poor.

Description

Technical Field

[0001] This invention belongs to the field of soil remediation technology, and in particular relates to a method for preparing a soil ecological remediation bio-fertilizer. Background Technology

[0002] In recent decades, with the rapid advancement of chemical technology, chemical fertilizers have quickly become dominant in agricultural production. However, the excessive use of chemical fertilizers has led to a series of problems. First, the efficiency of chemical fertilizer production in agriculture has declined significantly, while the amount used continues to rise. Second, soil compaction, decreased fertility, and acidification have occurred. Third, excessive use of chemical fertilizers has caused environmental pollution. The United Nations' report, "The State of the World's Soil Resources," highlights ten major threats to soil function: soil erosion, loss of soil organic carbon, nutrient imbalance, soil acidification, soil pollution, waterlogging, soil compaction, surface hardening, soil salinization, and loss of soil biodiversity. Soil pollution and degradation caused by the excessive application of pesticides and fertilizers have played a significant role.

[0003] Currently, there are many bio-fertilizers on the market, but their soil remediation effects are poor, mainly due to the single strain of microorganisms and insufficient fertilizer efficiency. The fruit shells, branches, leaves, and oilseed cakes of *Xanthoceras sorbifolium* contain a variety of nutrients, including abundant protein, more than 16 kinds of amino acids, and 12 kinds of trace elements essential to the human body. Multiple microorganisms can be cultured together to provide more nutrients. Furthermore, the fruit shells and oilseed cakes account for 75% of the harvest of *Xanthoceras sorbifolium*, which has not yet been utilized, making them an ideal substrate for producing bio-fertilizers. Summary of the Invention

[0004] In view of the above situation and to overcome the shortcomings of the prior art, the purpose of this invention is to provide a method for preparing soil ecological restoration bio-fertilizer, which aims to at least solve the problem of poor soil improvement effect of traditional microbial fertilizers.

[0005] The technical solution adopted in this invention is as follows: A method for preparing a soil ecological restoration bio-fertilizer according to this invention includes the following steps:

[0006] Step 1:

[0007] Collect branches and leaves pruned from the *Xanthoceras sorbifolium* tree, as well as the fruit shells removed when harvesting *Xanthoceras sorbifolium* seeds and the cake residue formed from processing edible oil;

[0008] Step Two:

[0009] All collected waste from *Sapindus mukorossi* fruit was put into a mixing and pulverizing machine and mixed and pulverized to obtain pulverized material;

[0010] Step 3:

[0011] Steam sterilization is used to eliminate natural bacteria in the pulverized material;

[0012] Step Four:

[0013] Add a combination of fermentation agents to the sterilized pulverized material to obtain fermented material;

[0014] Step 5:

[0015] Control the temperature and humidity of the fermentation material;

[0016] Step Six:

[0017] Place the fermenting material in a well-ventilated environment for 30 days to ferment;

[0018] Step Seven:

[0019] The nutrient content of the fermented feed was tested.

[0020] Step 8:

[0021] Add inorganic nutrients based on the test data in step seven, and adjust the ratio of N, P, and K.

[0022] Furthermore, in step five, the humidity is 50%-60% and the temperature is greater than or equal to 55°C.

[0023] Furthermore, the detection data in step seven includes total nutrient content, organic matter content, number of viable bacteria, moisture content, pH value, and ascarid mortality rate.

[0024] Furthermore, in step eight, the ratio of N, P, and K is 6:3:1.

[0025] A soil ecological restoration bio-fertilizer, wherein the combined fermentation agent is composed of Bacillus subtilis, Pichia pastoris, endophytic fungi Pseudomonas aeruginosa and Aspergillus niger in a ratio of 4:2:2:2.

[0026] After adopting the above technical solution, the beneficial effects of the present invention are as follows: It provides a method for preparing soil ecological restoration bio-fertilizer, which utilizes the organic matter and nutrients in the fruit shell and cake of *Xanthoceras sorbifolium* to effectively improve soil structure when used as fertilizer. At the same time, a combined fermentation agent composed of *Bacillus subtilis*, *Pichia pastoris*, endophytic fungi *Pseudomonas aeruginosa*, and *Aspergillus niger* is added for fermentation, so that the microorganisms and nutrients in the fermented fertilizer meet the standards for soil restoration. The ratio of N, P, and K is adjusted to obtain a bio-fertilizer that can effectively improve the soil and thus promote plant growth. Attached Figure Description

[0027] Figure 1 This is a process flow diagram of the preparation method of a soil ecological restoration bio-fertilizer according to the present invention. Detailed Implementation

[0028] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0029] Example 1:

[0030] This invention discloses a method for preparing a soil ecological restoration bio-fertilizer, comprising the following steps:

[0031] Step 1:

[0032] Collect branches and leaves pruned from the *Xanthoceras sorbifolium* tree, as well as the fruit shells removed when harvesting *Xanthoceras sorbifolium* seeds and the cake residue formed from processing edible oil;

[0033] Step Two:

[0034] All collected waste from *Sapindus mukorossi* fruit was put into a mixing and pulverizing machine and mixed and pulverized to obtain pulverized material;

[0035] Step 3:

[0036] Steam sterilization is used to eliminate natural bacteria in the pulverized material;

[0037] Step Four:

[0038] Add a combined fermentation agent to the sterilized pulverized material. The combined fermentation agent consists of Bacillus subtilis, Pichia pastoris, endophytic fungi Pseudomonas aeruginosa, and Aspergillus niger to obtain the fermented product. The ratio of the combined fermentation agent is 4:2:2:2.

[0039] Step 5:

[0040] Control the temperature and humidity of the fermentation material, with humidity at 50%-60% and temperature at 55℃ or higher.

[0041] Step Six:

[0042] Place the fermenting material in a well-ventilated environment for 30 days to ferment;

[0043] Step 7: Test the nutritional content of the fermented feed. The test data include total nutrient content, organic matter content, number of viable bacteria, moisture, pH value and ascarid mortality rate.

[0044] Step 8: Add inorganic nutrients based on the test data in Step 7, and adjust the ratio of N, P, and K to 6:3:1.

[0045] As demonstrated by the experiment in Example 1, when the product design meets the following standards: total nutrient content ≥10%, organic matter content ≥25%, effective live bacteria count ≥0.2 billion / gram, moisture content ≤30%, pH value between 5.5 and 7.5, and ascarid mortality rate ≥95%, ecological restoration of the soil can be achieved, and the product is considered qualified.

[0046] The present invention and its embodiments have been described above. This description is not restrictive, and the actual structure is not limited thereto. In conclusion, if those skilled in the art are inspired by this description and, without departing from the spirit of the invention, design similar structures and embodiments without creative effort, all such designs should fall within the protection scope of the present invention.

Claims

1. A method for preparing a soil ecological restoration bio-fertilizer, characterized in that, Includes the following steps: Step 1: Collect branches and leaves pruned from the *Xanthoceras sorbifolium* tree, as well as the fruit shells removed when harvesting *Xanthoceras sorbifolium* seeds and the cake residue formed from processing edible oil; Step Two: All collected waste from *Sapindus mukorossi* fruit was put into a mixing and pulverizing machine and mixed and pulverized to obtain pulverized material; Step 3: Steam sterilization is used to eliminate natural bacteria in the pulverized material; Step Four: Add a combination of fermentation agents to the sterilized pulverized material to obtain fermented material; Step 5: Control the temperature and humidity of the fermentation material; Step Six: Place the fermenting material in a well-ventilated environment for 30 days to ferment; Step Seven: The nutrient content of the fermented feed was tested. Step 8: Add inorganic nutrients based on the test data in step seven, and adjust the ratio of N, P, and K.

2. The method for preparing soil ecological restoration bio-fertilizer according to claim 1, characterized in that: The humidity in step five is 50%-60%, and the temperature is greater than or equal to 55℃.

3. The method for preparing soil ecological restoration bio-fertilizer according to claim 1, characterized in that: The data detected in step seven include total nutrient content, organic matter content, number of viable bacteria, moisture content, pH value, and ascarid mortality rate.

4. The method for preparing soil ecological restoration bio-fertilizer according to claim 1, characterized in that: In step eight, the ratio of N, P, and K is 6:3:

1.

5. The soil ecological restoration bio-fertilizer according to any one of claims 1-4, wherein the combined fermentation agent is composed of Bacillus subtilis, Pichia pastoris, endophytic fungus Pseudomonas aeruginosa, and Aspergillus niger in a ratio of 4:2:2:2.

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