Skin cleaning composition for mildly shrinking pores and removing blackheads, handmade soap as well as preparation method and application of handmade soap

Abstract

The invention discloses a skin cleaning composition for mildly shrinking pores and removing blackheads, handmade soap as well as a preparation method and application thereof, the skin cleaning composition comprises an oil phase and further comprises the following components in parts by weight: 5-10 parts of bakuchiol and 10-20 parts of ethyl linoleate; 5-10 parts of keratinase and 10-20 parts of silicon dioxide microspheres; 5 to 10 parts of porphyridium polysaccharide; according to the invention, propionibacterium acnes can be inhibited, and inflammation caused by bacterial infection can be reduced from the source; sebum composition is adjusted, and excessive sebum secretion is inhibited; mild cutin removal is achieved, chemical stimulation is avoided, accumulated cutin and dirt are removed by penetrating into pores, and generation of blackheads is reduced; under the synergistic effect of multiple angles, the effects of shrinking pores and removing blackheads can be well achieved, and the traditional Chinese medicine composition has a good application prospect in the field of daily chemical products.

Description

Technical Field

[0001] This invention relates to the field of daily cosmetics and cleaning products, specifically to a gentle handmade soap that shrinks pores and removes blackheads, and its preparation method. Background Technology

[0002] Blackheads (open comedones) are generally considered to be plug-like substances composed of sebum, dead skin cells, and bacteria. They are primarily caused by excessive sebum secretion from the sebaceous glands, abnormal keratinization of the hair follicles, and oxidation. The formation process involves excess sebum accumulating in the pores, enlarging them as the sebum encapsulates the skin, becoming a whitehead. When the whitehead breaks through the skin and oxidizes upon contact with air, it turns black. This is accompanied by the proliferation of Propionibacterium acnes, triggering an inflammatory response, forming blackheads, and in more severe cases, developing into acne.

[0003] There are numerous blackhead removal products on the market, with varying degrees of effectiveness. Traditional blackhead removal shampoos and conditioners are generally developed using highly acidic systems, resulting in significant irritation. To avoid this drawback, many researchers are using plant extracts to replace highly acidic substances in the development of gentler blackhead removal products. Their main mechanism is mostly to inhibit sebaceous gland enlargement, reduce oil secretion, and suppress Propionibacterium acnes to achieve the goal of removing blackheads.

[0004] Chinese invention patent application CN117462423A discloses a blackhead removal composition, a blackhead removal skincare product, and its preparation method, comprising the following components: 13-18 parts of Ili hydromica, 2.5-7.5 parts of plant liposomes, 4.5-7 parts of sodium stearate, 0.2-0.4 parts of Moringa seed oil, and 0.1-0.3 parts of asiaticoside. The plant liposomes are liposome-encapsulated extracts of witch hazel, sheep's milk root, burdock root, white willow bark, chrysanthemum, magnolia, schisandra fruit, Japanese raisin tree fruit, and peppermint. The key to this solution is that the small-molecule oils of Moringa seed oil and Ili hydromica reduce pore oil; the plant liposomes increase the moisture content of the stratum corneum, strengthen the skin barrier, and improve skin oil production, thereby reducing the likelihood of blackhead formation; finally, asiaticoside plays an antibacterial, cleansing, and anti-inflammatory role.

[0005] Chinese invention patent CN116270371B discloses a gentle pore-shrinking blackhead removal composition, blackhead-removing liquid, nose strips, and daily chemical products, comprising soapwort extract, peppermint leaf extract, salvia miltiorrhiza extract, and clove extract in a ratio of 5-10:15-25:0.6-1.2:0.6-1.2. This composition removes blackheads by gently shrinking pores, while simultaneously preventing blackhead recurrence through antibacterial action, sebum inhibition, and improved penetration of active ingredients into tissues. Clove extract enhances the skin penetration of the other three ingredients, improving their efficacy; salvia miltiorrhiza extract neutralizes the irritation of soapwort extract, making the composition gentle and non-irritating.

[0006] Chinese invention patent CN109055048B discloses a pure natural moisturizing and acne-reducing handmade soap, its preparation method, and its uses. The soap comprises: 20-30 parts shea butter and / or sweet almond oil and / or macadamia nut oil; 41-45 parts olive oil and / or grapeseed oil and / or sesame oil; 20-30 parts coconut oil and / or avocado oil and / or jojoba oil; 1.5-3 parts tea tree essential oil and / or lavender essential oil and / or ravensara leaf essential oil; 0.5-1 part white willow bark extract and / or natural salicylic acid; and 0.1-0.5 parts eucalyptus leaf oil and / or melaleuca truncatum essential oil and / or angelica essential oil. This handmade soap provides better moisturizing and acne-reducing effects for the skin.

[0007] The plant liposome components in the aforementioned invention patent or application document CN117462423A are difficult to apply to handmade soap products due to the influence of the pH environment of soap products on the stability of liposomes; CN116270371B lacks long-term lipid regulation, focuses on inhibiting sebaceous gland volume but does not change the composition of sebum, and the soapwort extract relies on saponification reaction, which has the risk of causing side effects. At the same time, the antibacterial components do not target the biofilm of Propionibacterium acnes and cannot penetrate to the deep layer; CN109055048B uses the method of directly compounding plant essential oils, but the essential oils are easy to volatilize during the handmade soap making process, resulting in a reduction in the content of effective ingredients in the final product. Summary of the Invention

[0008] The technical problem this invention aims to solve is how handmade soap can regulate sebum composition while inhibiting excessive sebum secretion, effectively control sebum production over a long period, and effectively inhibit bacteria through a complex antibacterial mechanism, while avoiding skin damage caused by chemical peels.

[0009] The skin cleansing composition according to some embodiments of this application includes, by weight, parts of... 5-10 parts of psoralen and 10-20 parts of ethyl linoleate.

[0010] 5-10 parts keratinase, 10-20 parts silica microspheres.

[0011] 5-10 parts of *Porphyra yezoensis* polysaccharide.

[0012] 10-15 parts of Dendrobium officinale peptide and 10-15 parts of cinnamaldehyde nanoemulsion.

[0013] The skin cleansing compositions according to some embodiments of this application also include 5 to 10 parts of European blueberry fruit extract.

[0014] The Dendrobium officinale peptide was prepared according to the skin cleansing composition described in some embodiments of this application, comprising: Add Dendrobium officinale powder to deionized water and treat with alkali for 30-60 minutes. The pH of the alkali treatment is 9.0-10.0 and the temperature is 90-100℃.

[0015] The first enzymatic hydrolysis was carried out by adding a complex glycosidase, wherein the mass ratio of cellulase, pectinase and amylase in the complex glycosidase was 1:(1~3):(3~5), the pH was 6.0~7.0, the temperature was 37~45℃, and the time was 1~2h.

[0016] A second enzymatic hydrolysis was performed by adding a complex protease, wherein the mass ratio of alkaline protease to papain in the complex protease was (1~3):1, the pH was 7.0~8.0, the temperature was 45~55℃, and the time was 2~4h.

[0017] Enzyme inactivation, filtration, concentration, freeze-drying, and other processes are used to obtain Dendrobium officinale peptides.

[0018] According to some embodiments of the skin cleansing composition of this application, wherein the preparation of the Dendrobium officinale peptide involves: According to some embodiments of the skin cleansing composition of this application, the mass ratio of cellulase, pectinase and amylase in the complex glycosidase is 1:2:4.

[0019] According to some embodiments of this application, the skin cleansing composition has a pH of 6.5, a temperature of 40°C, and a time of 2 hours.

[0020] According to some embodiments of the skin cleansing composition of this application, the mass ratio of alkaline protease to papain in the complex protease is 2:1.

[0021] According to some embodiments of this application, the skin cleansing composition has a pH of 7.5, a temperature of 50°C, and a time of 3 hours.

[0022] According to some embodiments of the skin cleansing composition of this application, the mass of the complex glycosidase is 1% to 3% of the mass of Dendrobium officinale powder.

[0023] According to the skin cleansing compositions in some embodiments of this application, the mass of the complex protease is 1% to 3% of the mass of Dendrobium officinale powder.

[0024] According to the skin cleansing compositions in some embodiments of this application, the Dendrobium officinale powder is obtained by pulverizing fresh or dried Dendrobium officinale and passing it through a 40-100 mesh sieve.

[0025] The *Plasma gondii* polysaccharide was prepared according to the skin cleansing compositions in some embodiments of this application, including... Alginate lyase was added to a phosphate buffer suspension of defatted Porphyromonas pulveratum powder for lysis. The lysis temperature was 45-50℃, the lysis time was 4-8 hours, and the pH was 7.0-8.0.

[0026] Enzyme inactivation, membrane separation, and collection of the filtrate, with a molecular weight cutoff of 3 kDa.

[0027] The filtrate was separated and purified using a Sephadex G-25 gel chromatography column with deionized water as the mobile phase, and the main peak fraction was collected.

[0028] The main peak fraction was frozen and dried to obtain polysaccharide from *Plasmodium purpureum*.

[0029] According to some embodiments of the skin cleansing composition of this application, wherein the preparation of the *Porphyra yezoensis* polysaccharide involves: The defatted purple algae powder is prepared according to the skin cleansing composition in some embodiments of this application, including... The purple algae was crushed and passed through a 50-70 mesh sieve to obtain purple algae powder.

[0030] The *Porphyra yezoensis* powder was extracted by reflux in hexane solvent to obtain the extract.

[0031] The extract was centrifuged and dried to obtain defatted purple algae powder.

[0032] The skin cleansing composition according to some embodiments of this application is passed through a 60-mesh sieve.

[0033] According to some embodiments of the skin cleansing composition of this application, the reflux extraction temperature is 40~60°C and the time is 1~3t.

[0034] According to some embodiments of the skin cleansing composition of this application, the reflux extraction temperature is 50°C and the time is 2t.

[0035] According to some embodiments of the skin cleansing composition of this application, the mass-to-volume ratio of the purple algae powder to the n-hexane solvent is 1:10.

[0036] According to some embodiments of the skin cleansing composition of this application, the centrifugation speed is 4000 rpm and the time is 15 min.

[0037] According to the skin cleansing compositions in some embodiments of this application, the phosphate buffer suspension of defatted Porphyra yezoensis powder is prepared by adding defatted Porphyra yezoensis powder to 0.1M phosphate buffer.

[0038] According to the skin cleansing composition in some embodiments of this application, a 2% (w / v) suspension of *Porphyra yezoensis* was prepared.

[0039] According to some embodiments of the skin cleansing compositions of this application, the molecular weight of the polysaccharide from *Plasmodium purpureum* is 1-5 kDa.

[0040] According to some embodiments of the skin cleansing composition of this application, wherein the preparation of the cinnamaldehyde nanoemulsion includes... Cinnamaldehyde, Span 80, and medium-chain triglycerides were mixed to obtain the oil phase.

[0041] Tween 80 dissolves in deionized water to obtain an aqueous phase.

[0042] The oil phase is added to the aqueous phase to obtain an emulsion. The emulsion is subjected to high-speed shearing, heating, and cooling to obtain the first cinnamaldehyde nanoemulsion.

[0043] According to some embodiments of the skin cleansing composition of this application, wherein the preparation of the cinnamaldehyde nanoemulsion includes: The preparation of the cinnamaldehyde nanoemulsion according to the skin cleansing composition of some embodiments of this application further includes... The cinnamaldehyde nanoemulsion is obtained by homogenization, wherein the average particle size of the cinnamaldehyde nanoemulsion is 50~80nm and the PDI < 0.2.

[0044] According to some embodiments of the skin cleansing composition of this application, cinnamaldehyde, Span 80, and medium-chain triglycerides are mixed in a water bath at 40-60°C until completely dissolved to obtain an oil phase.

[0045] According to some embodiments of the present application, the skin cleansing composition comprises cinnamaldehyde, Span 80, and medium-chain triglycerides mixed in a water bath at 50°C until completely dissolved to obtain an oil phase.

[0046] According to some embodiments of the present application, the skin cleansing composition is prepared by dissolving Tween 80 in deionized water and stirring at 40-60°C until transparent, thus obtaining an aqueous phase.

[0047] According to some embodiments of the present application, the skin cleansing composition is prepared by dissolving Tween 80 in deionized water and stirring at 40-60°C until transparent, thus obtaining an aqueous phase.

[0048] According to some embodiments of the skin cleansing composition of this application, the shearing speed is 9,000~11,000 rpm, the temperature is 40~60°C, and the time is 4~6 min.

[0049] According to some embodiments of the skin cleansing composition of this application, the shearing speed is 10,000 rpm, the temperature is 50°C, and the time is 5 min.

[0050] According to some embodiments of this application, the skin cleansing composition is heated to 80-90°C and then cooled to 20-30°C.

[0051] According to some embodiments of this application, the skin cleansing composition is heated to 85°C and then cooled to 25°C.

[0052] The skin cleansing compositions according to some embodiments of this application are homogenized using a high-pressure homogenizer.

[0053] According to some embodiments of the skin cleansing composition of this application, the keratinase is derived from Bacillus licheniformis and has an enzyme activity >800 U / g at 37°C.

[0054] According to some embodiments of the skin cleansing composition of this application, the porous silica microspheres have an average pore size of 10-20 nm and a specific surface area of ​​45-65 m². 2 / g, coefficient of friction 0.10~0.20.

[0055] According to some embodiments of the skin cleansing composition of this application, the anthocyanin content of the European blueberry fruit extract is >15%.

[0056] Skin cleansing compositions according to some embodiments of this application include handmade soaps.

[0057] According to some embodiments of the skin cleansing composition of this application, the handmade soap further includes an oil phase, which is 300-500 parts by weight.

[0058] According to some embodiments of the skin cleansing compositions of this application, the oil phase includes one or more of coconut oil, palm oil, olive oil, sweet almond oil, grapeseed oil, shea butter, avocado oil, tea seed oil, jojoba oil, rice bran oil, walnut oil, evening primrose oil, and flaxseed oil.

[0059] According to some embodiments of the skin cleansing composition of this application, the handmade soap further includes sodium hydroxide, in parts by weight, 30 to 60 parts.

[0060] According to some embodiments of the skin cleansing composition of this application, the handmade soap further includes 2 to 5 parts of disodium EDTA.

[0061] The method for preparing the handmade soap according to some embodiments of this application includes: S1. Add sodium hydroxide to water to obtain an alkaline solution, pour it into the oil phase at room temperature, stir, and obtain a soap solution.

[0062] S2. Add psoralen, ethyl linoleate, keratinase, porous silica microspheres, Dendrobium officinale peptide, cinnamaldehyde nanoemulsion, and Pseudocystis polysaccharide to the soap solution.

[0063] S3. The soap solution is molded to obtain the skin cleansing composition.

[0064] According to the skin cleansing composition in some embodiments of this application, step S3 further includes the addition of European blueberry fruit extract.

[0065] This invention also relates to a method for preparing Dendrobium officinale peptides to address the problem of skin antibacterial activity, the method comprising: Add Dendrobium officinale powder to deionized water and treat with alkali for 30-60 minutes. The pH of the alkali treatment is 9.0-10.0 and the temperature is 90-100℃. The first enzymatic hydrolysis was carried out by adding a complex glycosidase, wherein the mass ratio of cellulase, pectinase and amylase in the complex glycosidase was 1:(1~3):(3~5), the pH was 6.0~7.0, the temperature was 37~45℃, and the time was 1~2h. A second enzymatic hydrolysis was performed by adding a complex protease, wherein the mass ratio of alkaline protease to papain in the complex protease was (1~3):1, the pH was 7.0~8.0, the temperature was 45~55℃, and the time was 2~4h. Enzyme inactivation, filtration, concentration, freeze-drying, and other processes are used to obtain Dendrobium officinale peptides.

[0066] According to the method for preparing Dendrobium officinale peptides in some embodiments of this application, the mass ratio of cellulase, pectinase and amylase in the complex glycosidase is 1:2:4.

[0067] According to the method for preparing Dendrobium officinale peptides in some embodiments of this application, the pH is 6.5, the temperature is 40°C, and the time is 2 hours.

[0068] According to the method for preparing Dendrobium officinale peptides in some embodiments of this application, the mass ratio of alkaline protease to papain in the complex protease is 2:1.

[0069] According to the method for preparing Dendrobium officinale peptides in some embodiments of this application, the pH is 7.5, the temperature is 50°C, and the time is 3 hours.

[0070] According to the method for preparing Dendrobium officinale peptides in some embodiments of this application, the mass of the complex glycosidase is 1% to 3% of the mass of Dendrobium officinale powder.

[0071] According to the method for preparing Dendrobium officinale peptides in some embodiments of this application, the mass of the complex protease is 1% to 3% of the mass of Dendrobium officinale powder.

[0072] According to the method for preparing Dendrobium officinale peptides in some embodiments of this application, Dendrobium officinale powder is obtained by pulverizing fresh or dried Dendrobium officinale and passing it through a 40-100 mesh sieve.

[0073] Beneficial effects: This invention avoids the drawbacks of traditional acidic blackhead removal products that can easily damage the skin. By adding functional phases that regulate oil, deeply inhibit bacteria, gently exfoliate, and alleviate inflammation to the basic cleansing phase, it achieves a gentle and long-lasting effect in preventing and removing blackheads.

[0074] in: Oil-controlling group: Bakuchiol reduces dihydrotestosterone (DHT) production by inhibiting 5α-reductase activity, thus controlling oil production at its source. Ethyl linoleate replenishes linoleic acid in sebum, maintaining skin barrier function and regulating skin permeability. It also reverses abnormal follicular keratinization and reduces the thickness of the keratinocyte layer.

[0075] Bio-enzyme-physical synergistic exfoliation group: *Plasmodium purpureum* polysaccharides enhance the stability of keratinase, increasing its activity retention rate in a soap-based high-pH environment (pH 8.5~10.0). Keratinase selectively hydrolyzes keratin in keratin plugs, avoiding damage to the living cell layer. Porous silica microspheres soften and remove keratin through capillary adsorption and micro-friction, improving blackhead removal rate.

[0076] Targeted antibacterial group: Dendrobium officinale peptides inhibit the gene expression of Propionibacterium acnes, block the quorum sensing system, and inhibit biofilm matrix formation. Simultaneously, it promotes type I collagen synthesis and shrinks pores. Cinnamaldehyde nanoemulsions with a particle size of 50-80nm penetrate the biofilm matrix, disrupting the bacterial membrane phospholipid bilayer, and synergistically with Dendrobium officinale peptides to make the antibacterial effect more thorough.

[0077] Anti-inflammatory and repairing group: *Plasmodium purpureus* polysaccharide inhibits lipoxygenase (LOX) and reduces the synthesis of inflammatory mediators leukotrienes (LTs). When combined with *Blueberry* fruit extract, the inhibition rate is increased. *Blueberry* fruit extract inhibits the release of inflammatory factors, alleviates abnormal sebum secretion, abnormal follicular keratosis, and pore dilation caused by inflammation, and counteracts potential irritation from cleansing.

[0078] In this invention, multiple functional components work synergistically: Bakuchiol reduces DHT production and decreases sebum secretion; ethyl linoleate regulates sebum composition and improves follicular keratosis, providing a target site for keratinase; Porphyrin polysaccharide enhances the stability of keratinase in soap base and improves the keratin-reducing effect of keratinase; after keratinase softens keratin plugs, porous silica microspheres remove lipids through physical adsorption, avoiding the irritation of traditional acid exfoliation, and the natural glycerin produced by the saponification reaction also enhances the stability of keratinase.

[0079] Dendrobium officinale peptides inhibit the formation of biofilms of Propionibacterium acnes, while cinnamaldehyde nanoemulsions penetrate the biofilm to kill deep-seated bacteria. The synergistic effect of the two significantly reduces the number of viable bacteria in the biofilm. Dendrobium officinale peptides also have the effect of shrinking pores.

[0080] European blueberry extract inhibits the release of inflammatory factors, preventing recurrence of sebum secretion and follicular keratosis caused by inflammation. The combination of purple algae polysaccharide and European blueberry fruit extract further enhances the inhibitory effect of LTs.

[0081] The present invention, through the combined action of multiple components, fundamentally solves the blackhead problem caused by abnormal sebum secretion / composition, the growth of Propionibacterium acnes on the skin surface, and inflammation, while avoiding the potential risks of damaging the sebum barrier, damaging skin cells, and recurring blackheads. The resulting synergistic effect far exceeds the sum of single components or known combinations. Detailed Implementation

[0082] The present invention will be further described below with reference to the embodiments. It should be noted that the embodiments do not constitute a limitation on the scope of protection of the present invention.

[0083] To overcome the technical problems in the prior art, this invention provides a gentle handmade soap that shrinks pores and removes blackheads, and its preparation method. The resulting handmade soap product is gentle and non-irritating, effectively regulates sebum secretion, gently and precisely exfoliates, penetrates the biofilm of Propionibacterium acnes to thoroughly inhibit bacteria, suppresses skin inflammation, and has a four-fold synergistic network of oil control, antibacterial, anti-inflammatory, and cleansing, which has a significant effect on preventing and cleaning blackheads, thus achieving the goal of controlling blackheads from the root.

[0084] The present invention relates to a gentle, pore-minimizing, blackhead-removing handmade soap, comprising... The cleaning phase consists of 800-1000 parts of base oil, sodium hydroxide, and water, of which 300-500 parts are base oil, 30-60 parts are sodium hydroxide, and the remainder is water. The base oil is composed of one or more of the following: coconut oil, palm oil, olive oil, sweet almond oil, grapeseed oil, shea butter, avocado oil, tea seed oil, jojoba oil, rice bran oil, walnut oil, evening primrose oil, and flaxseed oil.

[0085] The oil-regulating functional phase consists of 15-30 parts, including 10-20 parts of ethyl linoleate and 5-10 parts of psoralen.

[0086] The antibacterial functional phase consists of 20-30 parts, including 10-15 parts of Dendrobium officinale peptide and 10-15 parts of cinnamaldehyde nanoemulsion.

[0087] The anti-inflammatory component consists of 10-20 parts, including 5-10 parts of *Plasmodium purpureum* polysaccharide and 5-10 parts of European blueberry fruit extract.

[0088] The exfoliating functional phase consists of 15-30 parts, including 5-10 parts of keratinase and 10-20 parts of porous silica microspheres. The auxiliary phase consists of 2-5 parts of disodium EDTA.

[0089] This invention avoids the drawbacks of traditional acidic blackhead removal products that can easily damage the skin. By adding functional phases that regulate oil, deeply inhibit bacteria, gently exfoliate, and alleviate inflammation to the basic cleansing phase, it achieves a gentle and long-lasting effect in preventing and removing blackheads.

[0090] Oil control adjustment group: Psoralen reduces the production of dihydrotestosterone (DHT) by inhibiting 5α-reductase activity, thus controlling oil production at the source of hormones.

[0091] Ethyl linoleate replenishes linoleic acid in sebum, maintaining skin barrier function and regulating skin permeability. It also reverses abnormal follicular keratosis and reduces the thickness of the keratinocyte layer.

[0092] Bio-enzyme-physical synergistic keratolysis group: The polysaccharide of *Plasmodium purpureum* enhances the stability of keratinase and improves the retention rate of keratinase activity in a soap-based high pH environment (pH 8.5~10.0).

[0093] Keratinase selectively hydrolyzes keratin in keratin plugs, avoiding damage to the living cell layer.

[0094] Porous silica microspheres soften keratin through capillary adsorption and micro-friction, thus improving the blackhead removal rate.

[0095] Targeted antibacterial group: Dendrobium officinale peptides inhibit gene expression in Propionibacterium acnes, block the quorum sensing system, and suppress biofilm matrix formation. Simultaneously, they promote type I collagen synthesis and shrink pores.

[0096] Cinnamaldehyde nanoemulsions with a particle size of 50-80nm penetrate the biofilm matrix, disrupt the bacterial membrane phospholipid bilayer, and work synergistically with Dendrobium officinale peptides to make the antibacterial effect more thorough.

[0097] Anti-inflammatory and repair group: Purple spirulina polysaccharide inhibits lipoxygenase (LOX) and reduces the synthesis of inflammatory mediators leukotrienes (LTs). The inhibition rate is increased when combined with European blueberry fruit extract.

[0098] European blueberry fruit extract inhibits the release of inflammatory factors, reduces abnormal sebum secretion, abnormal follicular keratosis, and enlarged pores caused by inflammation, and counteracts potential irritation from cleansing.

[0099] In one method of the present invention, the preparation of the Dendrobium officinale peptide includes... S110. Select fresh or dried Dendrobium officinale and crush it using a pulverizer, then pass it through a 40-100 mesh sieve.

[0100] S120. The obtained powder is mixed with deionized water, sodium hydroxide is added to adjust the pH to 9.0~10.0, and the mixture is treated with alkali at 90~100℃ for 30~60 min.

[0101] S130. A complex glycosidase consisting of cellulase, pectinase and amylase is used to enzymatically hydrolyze the solution. The mass ratio of cellulase, pectinase and amylase is 1:2:4, the pH is 6.0~7.0, the temperature is 37~45℃, the time is 1~2h, and the total amount used is 1%~3% of the mass of the raw material Dendrobium officinale.

[0102] S140. Add 1% to 3% of the mass of the raw material Dendrobium officinale to a compound protease for enzymatic hydrolysis. The mass ratio of alkaline protease to papain in the compound protease is 2:1, the pH is 7.0 to 8.0, the temperature is 45 to 55℃, and the time is 2 to 4 hours.

[0103] S150. After enzymatic hydrolysis, the enzyme is inactivated by heating, centrifugation and filtration are performed, and the solution with a solid content of 20-25% is obtained after membrane concentration.

[0104] S160. Dendrobium officinale peptide powder was obtained by freeze-drying.

[0105] Dendrobium officinale peptides inhibit the gene expression of Propionibacterium acnes, block the quorum sensing system, and inhibit biofilm matrix formation. Simultaneously, they promote type I collagen synthesis and shrink pores. In one method of this invention, the preparation of the cinnamaldehyde nanoemulsion includes... S210. Cinnamaldehyde, medium-chain triglycerides (MCT), and Span 80 are mixed in a 50°C water bath and magnetically stirred (500 rpm) until completely dissolved to form the oil phase.

[0106] S220. Tween 80 is added to deionized water, heated to 50°C and stirred until transparent to form the aqueous phase.

[0107] S230. Slowly pour the oil phase into the water phase and maintain a high-speed shear at 50°C (10,000 rpm for 5 minutes).

[0108] S240 is heated to 85°C and then rapidly cooled to 25°C, initiating a phase transition to form a nanoemulsion.

[0109] S250. The particle size was further reduced by a high-pressure homogenizer (15,000 psi, 3 cycles) to obtain a homogeneous emulsion. The average particle size of the nanoemulsion was 50-80 nm, and the dispersion PDI was <0.2.

[0110] Cinnamaldehyde nanoemulsions, with particle sizes of 50-80 nm, penetrate the biofilm matrix, disrupting the bacterial membrane phospholipid bilayer. This, combined with Dendrobium officinale peptides, enhances the antibacterial effect. While Dendrobium officinale peptides inhibit biofilm matrix formation, experiments show that adding Dendrobium officinale peptides alone is insufficient to effectively disrupt the biofilm formed by *C. acnes*. However, adding cinnamaldehyde nanoemulsions alone allows them to penetrate deep into the biofilm pores, disrupting membrane integrity by inserting their hydrophobic ends into the bacterial membrane phospholipid layer, thus exerting an antibacterial effect. The synergistic effect of both further reduces the biofilm's penetration barrier against antibacterial agents, resulting in better antibacterial rates.

[0111] In one method of the present invention, the preparation of the *Porphyra yezoensis* polysaccharide includes... S310. Select dried *Porphyra yezoensis* and pulverize it through a 60-mesh sieve. Extract the pulverized *Porphyra yezoensis* powder by reflux extraction with n-hexane solvent (1:10 w / v) at 50°C for 2 hours to remove lipid-soluble impurities. Centrifuge at 4000 rpm for 15 minutes. Collect the precipitate after centrifugation and air dry it naturally.

[0112] S320. Dissolve defatted *Porphyra yezoensis* powder in 0.1M phosphate buffer (pH 6.5~7.0) to obtain a suspension with a concentration of 2% (w / v). Then add alginate lyase, wherein the mass ratio of alginate lyase to defatted *Porphyra yezoensis* powder is 0.005:1. React at 45~50℃ for 4~8 hours at pH 7.0~8.0.

[0113] S330 is inactivated by heating in a 100℃ water bath for 10 minutes.

[0114] S340. Use an ultrafiltration membrane with a molecular weight cutoff of 3 kDa to fractionate the enzymatic hydrolysate and collect the filtrate.

[0115] S350. Using a Sephadex G-25 column (2.6×100cm) with deionized water as the mobile phase, the main peak fraction was collected and freeze-dried to obtain *Plasmodium purpureum* polysaccharide with a molecular weight of 1000~5000 Da.

[0116] Experiments showed that Porphyra yezoensis polysaccharide enhances the stability of keratinase and improves the retention rate of keratinase activity in a soap-based high pH environment (pH 8.5~10.0).

[0117] Preferably, the keratinase used in this invention is derived from Bacillus licheniformis, and its enzyme activity at 37°C is >800 U / g. The porous silica microspheres used in this invention have an average pore size of 10-20 nm and a specific surface area of ​​45-65 m². 2 / g, coefficient of friction 0.10~0.20. The anthocyanin content in the European blueberry fruit extract used in this invention is >15%.

[0118] Based on the above formula, the present invention also provides a method for preparing a gentle handmade soap that shrinks pores and removes blackheads, the specific steps of which are as follows: S1. Mix the base oil thoroughly according to a certain ratio. Place the solid oil in a 50°C water bath and heat and stir until completely melted, then mix well.

[0119] S2. Slowly add sodium hydroxide to water and stir until completely dissolved. Cool to room temperature (25~30℃). Slowly pour the alkali solution into the oil phase and continue stirring (500~1000 rpm) until the soap solution reaches a suitable consistency.

[0120] S3. Add psoralen, ethyl linoleate, keratinase, porous silica microspheres, Dendrobium officinale peptide, cinnamaldehyde nanoemulsion, Pseudocystis polysaccharide, and Vaccinium bracteatum extract in sequence, and homogenize (2000 rpm, 2 minutes).

[0121] S4. Pour the soap liquid into a silicone mold and let it stand at room temperature for 24-48 hours. After unmolding, place it in a cool, dry and ventilated place to cure for 4-8 weeks to obtain the handmade soap product.

[0122] In some specific embodiments, the psoralen, ethyl linoleate, keratinase, porous silica microspheres, and blueberry extract used in Examples 1-10 below were purchased from commercially available products. Dendrobium officinale peptides, Pseudolarix amabilis polysaccharides, and cinnamaldehyde nanoemulsions can also be purchased from commercially available products. The present invention preferably prepares them according to the following method: Preparation of Dendrobium officinale peptides: S110. Fresh Dendrobium officinale is selected and crushed using a pulverizer, then passed through a 40-mesh sieve.

[0123] S120. The powder is mixed with deionized water, sodium hydroxide is added to adjust the pH to 10.0, and the mixture is treated with alkali at 90℃ for 60 min.

[0124] S130. Add 1%–3% by mass of a compound glycosidase to the raw material (Dendrobium officinale powder) to the solution for enzymatic hydrolysis. The mass ratio of cellulase, pectinase, and amylase in the compound glycosidase is 1:2:4. The pH is 6.5, the temperature is 40℃, and the time is 2 hours.

[0125] S140. Add 1% to 3% of the raw material mass of a complex protease for enzymatic hydrolysis. The mass ratio of alkaline protease to papain in the complex protease is 2:1, the pH is 7.5, the temperature is 50℃, and the time is 3h.

[0126] S150. After enzymatic hydrolysis, the enzyme is inactivated by heating, centrifugation and filtration are performed, and the solution is concentrated through a membrane to obtain a solution with 25% solids.

[0127] S160. Dendrobium officinale peptide powder was obtained by freeze-drying.

[0128] Preparation of cinnamaldehyde nanoemulsion: S210. Mix cinnamaldehyde, MCT, and Span80 in a 50°C water bath and stir magnetically (500 rpm) until completely dissolved to form the oil phase.

[0129] Add S220.Tween80 to deionized water, heat to 50°C and stir until transparent to form the aqueous phase.

[0130] S230. Slowly pour the oil phase into the aqueous phase and maintain a high-speed shearing at 50°C (10,000 rpm, 5 min).

[0131] S240 is heated to 85°C and then rapidly cooled to 25°C, initiating a phase transition to form a nanoemulsion.

[0132] S250. The particle size was further reduced by a high-pressure homogenizer (15,000 psi, 3 cycles) to obtain a homogeneous emulsion. The nanoemulsion had an average particle size of 64.2 nm and a PDI of 0.16.

[0133] Preparation of polysaccharides from *Porphyra yezoensis*: S310. Select dried purple algae and crush it through a 60-mesh sieve.

[0134] S320. The pulverized *Plasmodium purpureum* powder was extracted by reflux extraction with hexane solvent (1:10 w / v) at 50°C for 2 hours to remove lipid-soluble impurities. The mixture was then centrifuged at 4000 rpm for 15 min. The precipitate was collected after centrifugation and air-dried naturally.

[0135] S330. Dissolve defatted *Porphyra yezoensis* powder in 0.1 M phosphate buffer (pH 7.0) to prepare a suspension with a powder concentration of 2%, thus obtaining a defatted *Porphyra yezoensis* powder solution. Add alginate lyase at a mass ratio of 0.005:1 to the defatted *Porphyra yezoensis* powder, react at 50°C for 8 hours, and maintain the pH at 7.0.

[0136] S340 is inactivated by heating in a 100℃ water bath for 10 minutes.

[0137] S350. Use an ultrafiltration membrane with a molecular weight cutoff of 3 kDa to fractionate the enzymatic hydrolysate and collect the filtrate.

[0138] S360. Using a Sephadex G-25 column (2.6×100cm) with deionized water as the mobile phase, the main peak fraction was collected and freeze-dried to obtain the polysaccharide of *Plasmodium purpureum*, with a weight-average molecular weight of 1358.24.

[0139] Examples 1-14: Examples 1-14 were implemented according to the handmade soap preparation method described in this invention. The cleaning phase consisted of 150 parts coconut oil, 150 parts palm oil, 300 parts olive oil, 100 parts sweet almond oil, 100 parts sodium hydroxide, and 200 parts deionized water.

[0140] The differences in formulations of the oil-regulating phase, antibacterial phase, anti-inflammatory phase, and exfoliating phase are shown in the table below. Handmade soap samples 1-14 of each embodiment were obtained according to the handmade soap preparation method described above, and were diluted with deionized water at a ratio of 1:10 to prepare sample solutions 1-14.

[0141] Experiment 1: Effects of sebum secretion inhibition and sebum composition regulation (SZ95 human sebaceous gland cell model): Sebaceous gland cells were arranged at 1×10⁻⁶ cells per pore. 4 Cells were seeded in 96-well plates and cultured overnight. After treatment, seven replicates were set up. Wells without cells served as the blank group, and wells with cells but no treatment served as the control group. After treatment with 0.1% sample solution 1, 2, 3, 13, and 14 for 48 hours, CCK-8 solution was added and incubated at 37°C for 2 hours. The absorbance at 450 nm was then measured.

[0142] The results showed that after 48 hours, there was no significant change in cell viability between the sample solution treatment groups and the control group, indicating good safety.

[0143] After treatment with 0.1% sample solutions 1, 2, 3, 13, and 14, the cells were centrifuged (3000 rpm, 10 min) to remove cell debris and supernatant. The cells were then stained with 10 g / mL Nile Red and 15 g / mL fluorescein diacetate diluted with phosphate-buffered saline (PBS), respectively, and incubated at 37°C in the dark for 10 min. After washing twice with PBS, the fluorescence intensity of Nile Red and fluorescein diacetate was detected using a multi-functional microplate reader (excitation wavelength 485 nm / emission wavelength 565 nm, excitation wavelength 494 nm / emission wavelength 523 nm).

[0144] The results are expressed as the ratio of Nile Red to fluorescein diacetate fluorescence intensity, and the percentage of the treatment group to the control group is used to represent the lipid synthesis rate. Simultaneously, GC-MS was used to determine the proportion of linoleic acid (C18:2) in the total fatty acids of the supernatant.

[0145] The results are shown in the table below. In the Nile Red staining experiment, in groups 1 and 14 without the addition of psoralen and ethyl linoleate, sebaceous cells secreted more fat, and the proportion of linoleic acid (C18:2) was lower. In group 2 with the addition of ethyl linoleate, although the level of fat secretion did not change significantly, the proportion of linoleic acid increased significantly. In group 3 with the addition of psoralen, the level of fat secretion decreased significantly, but the proportion of linoleic acid did not change significantly. In group 13, the level of fat secretion by sebaceous cells decreased significantly, while the proportion of linoleic acid increased.

[0146] Experimental Example 2: Skin keratinization and blackhead removal effects: (3×3) cm samples were taken from pig skin. 2 Seven pieces of excised skin were prepared. An artificial sebum was prepared using 50% palmitate glyceryl ester, 20% hexadecyl palmitic acid, 20% squalene, and 10% cholesterol. Stratum corneum collected from pig ear skin was ground into particles of 10-50 μm. The artificial sebum and stratum corneum particles were mixed in a 5:1 ratio, and 1g was evenly applied to the excised pig skin to simulate blackheads. Sample solutions 3, 4, 5, 6, 12, 13, and 14 were then massaged in a clockwise circular motion for 1 minute, followed by rinsing the skin three times with physiological saline. Paraffin sections of the skin were prepared using standard methods and stained with hematoxylin and eosin (HE). ImageJ image processing was used to analyze the stratum corneum thickness in the sections, and the skin color depth before and after sample treatment was compared to evaluate the blackhead removal rate. The results are shown below. Compared to groups 4 and 14 without keratinase and porous silica microspheres, the stratum corneum thickness of the sample solution group 13 was significantly reduced, and the blackhead removal rate was significantly improved. Furthermore, the effect was superior to that of sample solution group 5 (with porous microspheres only) and sample solution group 6 (with keratinase only). Meanwhile, compared with sample group 13, the lack of ethyl linoleate in sample group 3 and the lack of Porphyromonas auricula polysaccharide in sample group 12 showed a significant decrease in stratum corneum thickness and blackhead removal rate, indicating that ethyl linoleate and Porphyromonas auricula polysaccharide have a promoting effect on maintaining the stability of keratinase in soap base.

[0147] Experiment 3: Antibacterial effect: Brain and heart extract broth BHI medium containing 1% concentration of sample solutions 7, 8, 9, 13, and 14 was prepared. After sterilization, *C. acnes* bacteria activated to the logarithmic growth phase were inoculated into the medium at an inoculation rate of 2.0% (V / V) and anaerobic cultured (37℃, 5% CO2) for 48 hours. (Bacterial concentration adjusted to 1×10⁻⁶) 6CFU / mL samples were inoculated into different culture media and incubated at a constant temperature with shaking for 12 h (37℃, 200 rpm). Each group was repeated three times, with a negative control group without sample solution and a blank control group without inoculation. 200 μL of culture was aspirated into each well of a 96-well plate to determine A600, and the inhibition rate was calculated using the formula: Inhibition rate (%) = [1 - (Experimental group A600 - Blank group A600) ÷ (Negative group A600 - Blank group A600)] × 100%.

[0148] C. acnes cryopreservation was inoculated onto BHI agar plates and anaerobically cultured (37℃, 5% CO2) for 48 hours. Single colonies were picked and inoculated onto BHI liquid medium and anaerobically shaken (120 rpm) until OD600 = 0.6. The bacterial culture was then diluted to 1×10⁻⁶. 6 CFU / mL was inoculated into 6-well plates (2 mL per well) and anaerobically cultured for 72 hours to form a biofilm. Sample solutions 7, 8, 9, 13, and 14 were prepared to a final concentration of 1% and anaerobically incubated at 37°C for 24 hours. An equal volume of sterile PBS was added to the control group. The biofilm amount in each group was measured by crystal violet method to determine OD570; the higher the absorbance value, the higher the biofilm amount.

[0149] The antibacterial rate and OD570 results are shown below. Compared with the samples 7 and 14 without the addition of Dendrobium officinale peptide or cinnamaldehyde nanoemulsion, the sample groups 8 and 9 with added Dendrobium officinale peptide alone showed a significant improvement in the antibacterial rate against C. acnes. The sample group 9 with added Dendrobium officinale peptide alone had difficulty in effectively destroying the biofilm formed by C. acnes, while the sample group 8 with added cinnamaldehyde nanoemulsion alone could penetrate deep into the biofilm pores and exert its antibacterial effect by inserting the hydrophobic end into the phospholipid layer of the bacterial membrane to destroy the membrane integrity. The sample group 13 with the synergistic effect of the two further reduced the biofilm's permeation barrier to antibacterial agents and also showed better performance in terms of antibacterial rate.

[0150] Experiment 4: Blackhead Removal Efficacy Test: Oily skin was selected (the test area on the nose side had an oil content greater than 90μg / cm² before washing). 2Ninety-six subjects, aged 18-45 years, with noticeable pores on both cheeks and visible blackheads on both sides of the nose, were divided into 12 groups of 8 people each. They used samples 1-14, applying the samples once daily for cleansing for 28 days. Skin oil content was measured before using the test product, and full-face photos were taken on days 7 and 28. The number of blackheads on both sides of the nose was also assessed and counted. The number of subjects developing erythema after use was recorded, and the rate of change of each data point relative to before using the test product was calculated. The evaluation results were compared using statistical tests to determine if there were statistically significant differences. Test areas: number of blackheads on both sides of the nose, oil content on both sides of the nose (left / right), probability of erythema (mildness), and subject self-evaluation of the whole face (satisfaction).

[0151] The results are shown in the table below. Regarding facial oil, all sample groups showed varying degrees of improvement in facial oil content, with the order being: Sample 13 > Samples 10, 11, 12 > Samples 4, 5, 6 > Samples 7, 8, 9 > Samples 1, 2, 3 > Sample 14. This may be due to: 1) the synergistic regulation of sebaceous cells by psoralen and ethyl linoleate, reducing DHT production and sebum secretion; 2) the gentle exfoliation and pore-cleansing effects of keratinase and porous microspheres, which reduce sebum blockage, increased pressure within sebaceous glands, and stimulation of further sebum secretion; and 3) the inhibition of Propionibacterium acnes growth by Dendrobium officinale peptides and cinnamaldehyde nanoemulsion, preventing the accelerated decomposition of triglycerides in sebum and stimulating sebaceous gland cells to secrete sebum. Ultimately, Sample 13, with the synergistic effect of multiple components, demonstrated superior oil control compared to other experimental groups.

[0152] Regarding blackhead improvement, samples 11 and 13 were superior to samples 10 and 12, and significantly superior to samples 1, 2, and 3 (lacking sebum-regulating phase), samples 4, 5, and 6 (lacking keratin-improving phase), samples 7, 8, and 9 (lacking antibacterial phase), and the control group (handmade soap base). Excessive sebum secretion, accumulation and blockage at the hair follicle opening, excessive keratinization of keratinocytes, and inflammatory reactions caused by bacterial decomposition are all factors that contribute to severe blackheads. Multiple targets working together are needed for more effective blackhead improvement. Meanwhile, regarding the incidence of erythema, samples 10, 11, 12, and 14 all showed mild erythema. These were groups lacking *Plasmodium styracifolium* polysaccharide and / or *Vaccinium bracteatum* fruit extract. It is speculated that some individuals with sensitive skin experienced mild discomfort while keratinization was being improved. The combination of *Vaccinium bracteatum* fruit extract and *Plasmodium styracifolium* polysaccharide reduced the inflammatory response by inhibiting the release of inflammatory mediators, thus decreasing the incidence of erythema.

[0153] This invention utilizes Dendrobium officinale peptides and cinnamaldehyde nanoemulsion to inhibit Propionibacterium acnes, reducing inflammation caused by bacterial infection at its source; ethyl linoleate regulates sebum composition, and psoralen inhibits excessive sebum secretion; keratinase gently exfoliates, and porous microspheres physically adsorb to avoid chemical irritation, deeply removing accumulated keratin and dirt from pores, reducing blackhead formation; and Pseudocystis polysaccharides and Bilberry fruit extract alleviate skin inflammation. This invention, through synergistic effects from multiple angles, effectively shrinks pores and removes blackheads, showing promising application prospects in the daily chemical products field.

[0154] In summary, the handmade soap provided by this invention has excellent pore-shrinking and blackhead-removing effects, is fast-acting, does not damage the skin, and does not cause allergic reactions, making it suitable for facial cleansing applications. Finally, it should be noted that the above examples are merely a few specific embodiments of this invention. All derivatives that can be directly derived or conceived by those skilled in the art from the content disclosed in this invention should be considered within the scope of protection of this invention.

Claims

1. A skin cleansing composition, characterized in that, By weight, including 5-10 parts of psoralen and 10-20 parts of ethyl linoleate; 5-10 parts keratinase, 10-20 parts silica microspheres; 5-10 parts of *Porphyra yezoensis* polysaccharide; 10-15 parts of Dendrobium officinale peptide and 10-15 parts of cinnamaldehyde nanoemulsion.

2. The skin cleansing composition according to claim 1, characterized in that, It also includes 5-10 parts of European blueberry fruit extract; Preferably, the anthocyanin content in the European blueberry fruit extract is >15%; preferably, the keratinase is derived from Bacillus licheniformis, and its enzyme activity at 37°C is >800 U / g; preferably, the silica microspheres have an average pore size of 10-20 nm and a specific surface area of ​​45-65 m². 2 / g, friction coefficient 0.10~0.

20.

3. The skin cleansing composition according to claim 1, characterized in that, Preparation of the Dendrobium officinale peptide includes Add Dendrobium officinale powder to deionized water and treat with alkali for 30-60 minutes. The pH of the alkali treatment is 9.0-10.0 and the temperature is 90-100℃. The first enzymatic hydrolysis was carried out by adding a complex glycosidase, wherein the mass ratio of cellulase, pectinase and amylase in the complex glycosidase was 1:(1~3):(3~5), the pH was 6.0~7.0, the temperature was 37~45℃, and the time was 1~2h. A second enzymatic hydrolysis was performed by adding a complex protease, wherein the mass ratio of alkaline protease to papain in the complex protease was (1~3):1, the pH was 7.0~8.0, the temperature was 45~55℃, and the time was 2~4h. Enzyme inactivation, filtration, concentration, freeze-drying, and other processes are used to obtain Dendrobium officinale peptides.

4. The skin cleansing composition according to claim 3, characterized in that, in, In the preparation of the Dendrobium officinale peptide: preferably, the mass ratio of cellulase, pectinase and amylase in the complex glycosidase is 1:2:4; preferably, the pH is 6.5, the temperature is 40℃, and the time is 2h; preferably, the mass ratio of alkaline protease and papain in the complex protease is 2:1; preferably, the pH is 7.5, the temperature is 50℃, and the time is 3h. Preferably, the mass of the compound glycosidase is 1% to 3% of the mass of Dendrobium officinale powder; preferably, the mass of the compound protease is 1% to 3% of the mass of Dendrobium officinale powder; preferably, the Dendrobium officinale powder is obtained by pulverizing fresh or dried Dendrobium officinale and passing it through a 40-100 mesh sieve.

5. The skin cleansing composition according to claim 1, characterized in that, Preparation of the aforementioned *Porphyra yezoensis* polysaccharide includes: Alginate lyase was added to a phosphate buffer suspension of defatted Porphyromonas pulveratum powder for lysis. The lysis temperature was 45-50℃, the lysis time was 4-8 hours, and the pH was 7.0-8.

0. Enzyme inactivation, membrane separation, and collection of the filtrate, with a molecular weight cutoff of 3 kDa; The filtrate was separated and purified using a Sephadex G-25 gel chromatography column with deionized water as the mobile phase, and the main peak fraction was collected. The main peak fraction was frozen and dried to obtain polysaccharide from *Plasmodium purpureum*.

6. The skin cleansing composition according to claim 5, characterized in that, in, In the preparation of the *Porphyra yezoensis* polysaccharide: the preparation of the defatted *Porphyra yezoensis* powder includes pulverizing *Porphyra yezoensis*, passing it through a 50-70 mesh sieve to obtain *Porphyra yezoensis* powder; and refluxing the *Porphyra yezoensis* powder in n-hexane solvent to obtain an extract; The extract was centrifuged and dried to obtain defatted Porphyra yezoensis powder; Preferably, the material is passed through a 60-mesh sieve; preferably, the reflux extraction temperature is 40~60℃ and the time is 1~3t; more preferably, the reflux extraction temperature is 50℃ and the time is 2t; preferably, the mass-to-volume ratio of the *Porphyra yezoensis* powder to the hexane solvent is 1:10; preferably, the centrifugation speed is 4000rpm and the time is 15min; preferably, the phosphate buffer suspension of defatted *Porphyra yezoensis* powder is prepared by adding defatted *Porphyra yezoensis* powder to 0.1M phosphate buffer; more preferably, a 2% (w / v) *Porphyra yezoensis* suspension is prepared; preferably, the molecular weight of *Porphyra yezoensis* polysaccharide is 1~5kDa.

7. The skin cleansing composition according to claim 1, characterized in that, Preparation of the cinnamaldehyde nanoemulsion includes Cinnamaldehyde, Span 80, and medium-chain triglycerides were mixed to obtain the oil phase. Tween 80 dissolves in deionized water to form an aqueous phase. The oil phase is added to the aqueous phase to obtain an emulsion. The emulsion is subjected to high-speed shearing, heating, and cooling to obtain the first cinnamaldehyde nanoemulsion.

8. The skin cleansing composition according to claim 7, characterized in that, in, In the preparation of the cinnamaldehyde nanoemulsion: the preparation of the cinnamaldehyde nanoemulsion further includes homogenizing the first cinnamaldehyde nanoemulsion to obtain the cinnamaldehyde nanoemulsion, wherein the average particle size of the cinnamaldehyde nanoemulsion is 50~80nm and PDI<0.

2. Preferably, cinnamaldehyde, Span 80, and medium-chain triglycerides are mixed in a water bath at 40-60°C until completely dissolved to obtain an oil phase; more preferably, cinnamaldehyde, Span 80, and medium-chain triglycerides are mixed in a water bath at 50°C until completely dissolved to obtain an oil phase; preferably, Tween 80 is dissolved in deionized water and stirred at 40-60°C until transparent to obtain an aqueous phase; more preferably, Tween 80 is dissolved in deionized water and stirred at 50°C until transparent to obtain an aqueous phase. Preferably, the shearing speed is 9,000~11,000 rpm, the temperature is 40~60℃, and the time is 4~6 min; more preferably, the shearing speed is 10,000 rpm, the temperature is 50℃, and the time is 5 min; preferably, the temperature is raised to 80~90℃ and cooled to 20~30℃; preferably, the temperature is raised to 85℃ and cooled to 25℃; preferably, homogenization is performed by a high-pressure homogenizer.

9. The skin cleansing composition according to any one of claims 1-8, characterized in that, The soap includes handmade soap, which further includes an oil phase, comprising 300-500 parts by weight. Preferably, the oil phase includes one or more of the following: coconut oil, palm oil, olive oil, sweet almond oil, grapeseed oil, shea butter, avocado oil, tea seed oil, jojoba oil, rice bran oil, walnut oil, evening primrose oil, and flaxseed oil. Preferably, the handmade soap further includes sodium hydroxide, in parts by weight, 30-60 parts; Preferably, the handmade soap further includes 2-5 parts of disodium EDTA.

10. A method for preparing the handmade soap of claim 9, comprising: S1. Add sodium hydroxide to water to obtain an alkaline solution, pour it into the oil phase at room temperature, stir, and obtain a soap solution; S2. Add psoralen, ethyl linoleate, keratinase, porous silica microspheres, Dendrobium officinale peptide, cinnamaldehyde nanoemulsion, and Pseudocystis polysaccharide to the soap solution; S3. The soap solution is molded to obtain the skin cleansing composition; Preferably, step S3 further includes the addition of European blueberry fruit extract.

11. A method for preparing Dendrobium officinale peptides, characterized in that, include Add Dendrobium officinale powder to deionized water and treat with alkali for 30-60 minutes. The pH of the alkali treatment is 9.0-10.0 and the temperature is 90-100℃. The first enzymatic hydrolysis was carried out by adding a complex glycosidase, wherein the mass ratio of cellulase, pectinase and amylase in the complex glycosidase was 1:(1~3):(3~5), the pH was 6.0~7.0, the temperature was 37~45℃, and the time was 1~2h. A second enzymatic hydrolysis was performed by adding a complex protease, wherein the mass ratio of alkaline protease to papain in the complex protease was (1~3):1, the pH was 7.0~8.0, the temperature was 45~55℃, and the time was 2~4h. Enzyme inactivation, filtration, concentration, freeze-drying, and other processes are used to obtain Dendrobium officinale peptides.

12. The method for preparing Dendrobium officinale peptides according to claim 11, characterized in that, Preferably, the mass ratio of cellulase, pectinase and amylase in the compound glycosidase is 1:2:4; Preferably, the pH is 6.5, the temperature is 40°C, and the time is 2 hours; Preferably, the mass ratio of alkaline protease to papain in the complex protease is 2:1; Preferably, the pH is 7.5, the temperature is 50°C, and the time is 3 hours; Preferably, the mass of the compound glycosidase is 1% to 3% of the mass of Dendrobium officinale powder; Preferably, the mass of the compound protease is 1% to 3% of the mass of Dendrobium officinale powder; Preferably, the Dendrobium officinale powder is obtained by crushing fresh or dried Dendrobium officinale and passing it through a 40-100 mesh sieve.

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