High-integration multi-mode fluorescence dynamics test system and method

Abstract

The invention discloses a high-integration multi-mode fluorescence dynamics testing system and method, and relates to an optical detection and imaging technology. A high-integration and multi-mode test system composed of a laser beam modulation module, an LED beam modulation module, a multi-mode function configuration module, a sample upright excitation module, a sample side excitation module, a sample inverted excitation module, a digital image acquisition module, a spectral resolution acquisition module and a time domain response acquisition module is built. The use state of each sub-module of the multi-mode function configuration module is switched; a high-integration and multi-mode test system is controlled to carry out digital image, spectral resolution and time domain response acquisition in any one of a single / double-beam laser upright excitation mode, a single / double-beam laser side excitation mode, a single-beam LED upright excitation mode, a single-beam LED side excitation mode and a single-beam laser inverted excitation mode. A multi-mode test function is integrally designed, the limitation of single-mode detection is solved, the efficiency is improved, and the total cost of the system is reduced.

Description

Technical Field

[0001] This invention relates to the field of optical detection and imaging technology, and in particular to a highly integrated optical testing platform applicable to various types of luminescent materials. Specifically, it relates to an integrated system that combines functions such as laser / LED multi-source excitation, upright / lateral / inverted multi-modal excitation structure, image acquisition, spectral analysis, and time-resolved detection. Background Technology

[0002] In recent years, luminescent materials have been increasingly widely used in life sciences, materials science, environmental monitoring, information storage, and optoelectronic devices. Different types of luminescent materials, such as fluorescent dyes, quantum dots, upconversion nanoparticles, and long-afterglow materials, exhibit complex and diverse luminescence behaviors, which urgently require analysis of their excitation response characteristics using sophisticated optical methods, including multi-dimensional information such as their luminescence images, emission spectra, and fluorescence lifetimes. However, most existing optical testing equipment has one or more of the following prominent problems: (1) Single-mode design: Most current equipment only supports upright excitation or side excitation, which cannot adapt to the detection needs of different morphological samples (such as attached cells, solution systems, transparent cavities, microfluidic chips, etc.); (2) Module separation redundancy: Image acquisition, spectrum acquisition, time-domain lifetime acquisition and other functions need to be completed by independent equipment, which makes it difficult to synchronize data between equipment, cumbersome to calibrate, and lengthy experimental procedures; (3) Poor flexibility of light source and excitation: Existing equipment generally only supports single-wavelength laser or LED light sources, which cannot select the optimal excitation band and beam shape according to the excitation spectrum of the material, and it is difficult to achieve polarization control; (4) Lack of integrated structure: The system structure is complex and lacks a unified control platform, which leads to low experimental efficiency, error accumulation, serious repeated testing, and difficulty in achieving visualization, intelligent or high-throughput detection expansion.

[0003] Therefore, there is an urgent need to develop a new type of testing system with multimodal excitation, flexible light source control, imaging-spectral-lifetime linkage acquisition, and high integration to meet the pressing needs of comprehensive analysis of modern multi-type luminescent materials. Summary of the Invention

[0004] The purpose of this invention is to address the shortcomings of existing technologies by providing a highly integrated multimodal fluorescence dynamics testing system and method, relating to the field of optical detection and imaging technology. It overcomes the deficiencies of existing technologies by implementing a multimodal excitation path switching design, enabling the coordinated acquisition of multiple information such as image spatial distribution, emission spectrum characteristics, and luminescence dynamics, greatly improving the completeness, analytical depth, and research efficiency of experimental data, and achieving multidimensional fusion of optical information.

[0005] To achieve the above objectives, the present invention provides the following technical solution:

[0006] A highly integrated multimodal fluorescence dynamics testing system and method includes: a first laser beam modulation module, a second laser beam modulation module, an LED beam modulation module, a multimodal function configuration module, a sample upright excitation module, a sample side excitation module, a sample inverted excitation module, a digital image acquisition module, a spectral resolution acquisition module, and a time-domain response acquisition module. By switching the usage status of each sub-module of the multimodal function configuration module, digital image, spectral resolution, and time-domain response acquisition are performed in any of the following modes: single / dual-beam laser upright excitation mode, single / dual-beam laser side excitation mode, single-beam LED upright excitation mode, single-beam LED side excitation mode, and single-beam laser inverted excitation mode.

[0007] The single / dual-beam laser upright excitation mode includes a first laser beam modulation module, a second laser beam modulation module, a multi-modal function configuration module, a sample upright excitation module, a digital image acquisition module, a spectral resolution acquisition module, and a time-domain response acquisition module; the single / dual-beam laser side excitation mode includes a first laser beam modulation module, a second laser beam modulation module, a multi-modal function configuration module, a sample side excitation module, a digital image acquisition module, a spectral resolution acquisition module, and a time-domain response acquisition module; the single-beam LED upright excitation mode includes an LED beam modulation module, a multi-modal function configuration module, a sample upright excitation module, a digital image acquisition module, a spectral resolution acquisition module, and a time-domain response acquisition module; The system comprises a digital image acquisition module, a spectral resolution acquisition module, and a time-domain response acquisition module; the single-beam LED-side excitation mode includes an LED beam modulation module, a multi-modal function configuration module, a sample-side excitation module, a digital image acquisition module, a spectral resolution acquisition module, and a time-domain response acquisition module; the single-beam laser inverted excitation mode includes a first laser beam modulation module, a multi-modal function configuration module, a sample inverted excitation module, a digital image acquisition module, a spectral resolution acquisition module, and a time-domain response acquisition module; the camera imaging, emission spectrum, and time-domain response acquisition functions for any mode are achieved by changing the sub-modules in the multi-modal function configuration module;

[0008] In the single / dual-beam laser upright excitation mode, single / dual-beam laser side excitation mode, and single-beam laser inverted excitation mode, the first laser beam modulation module includes a first laser source unit, a first wavelength selection component, a first beam quality control unit, and a first polarization state control unit. The first laser source unit generates a continuous / pulsed laser beam output with a wavelength matched to the ground state absorption peak of the sample to excite specific energy level transitions; the first wavelength selection component is used to purify the laser, and the first beam quality control unit optimizes the beam profile, stability, and spatial coherence; the first polarization state control unit modulates the polarization state of the laser to match the optical anisotropy characteristics or polarization selective response of the sample, and finally inputs it into the multi-mode function configuration module; the second laser beam modulation module includes a second laser source unit, a second wavelength selection component, a second beam quality control unit, and a second polarization state control unit. The second laser source unit generates a continuous / pulsed laser beam output with a wavelength that matches the ground state absorption peak of the sample to excite specific energy level transitions; the second wavelength selection component is used to purify the laser, and the beam profile, stability and spatial coherence are optimized by the second beam quality control unit; the second polarization state control unit modulates the polarization state of the laser to match the optical anisotropy characteristics or polarization selective response of the sample, and finally inputs it into the multi-mode function configuration module.

[0009] In the single-beam LED upright excitation mode and the single-beam LED side excitation mode, the LED beam modulation module includes a broadband light source unit, a third wavelength selection component, and a third beam quality control unit. The broadband light source unit provides stable and adjustable broadband or narrow-spectrum LED output under non-laser conditions; the third wavelength selection component precisely defines the excitation band and avoids interference from non-target wavelengths; the third beam quality control unit shapes the divergent light of the LED into a beam profile, illumination angle, and beam intensity distribution suitable for exciting the sample, and finally inputs it into the multi-mode function configuration module.

[0010] The multimodal function configuration module contains several sub-modules (first sub-module, second sub-module, third sub-module, fourth sub-module, fifth sub-module, sixth sub-module, and seventh sub-module). Each sub-module houses an optical path deflection component for altering the propagation direction of the excitation light and emission signal. The third sub-module is used to directionally import the emission signal into the digital image acquisition module, enabling the capture and digital processing of emission image signals from the sample under different excitation modes. The second sub-module is used to directionally import the emission signal into the spectral resolution acquisition module, enabling high-sensitivity acquisition of emission spectral data from the sample in the excitation state and real-time analysis of the wavelength response characteristics generated during emission. The first sub-module is used to directionally import the emission signal into the time-domain response acquisition module, enabling the acquisition and analysis of various time-domain characteristics of the light signal, such as rise time and lifetime, as it evolves over time.

[0011] In the single / dual-beam laser upright excitation mode and the single-beam LED upright excitation mode, the sample upright excitation module includes an upright objective lens unit and an upright three-dimensional sample manipulation platform. The excitation light, after passing through the multi-modal function configuration module, is focused by the upright objective lens unit to excite the sample held by the upright three-dimensional sample manipulation platform. The emitted light enters the multi-modal function configuration module through the same upright objective lens unit and is directed to imaging acquisition, spectral resolution, time-domain response, and other functional modules according to experimental requirements. The upright objective lens unit is positioned above the sample and can be equipped with various types of objectives, such as high / low numerical aperture objectives, long / short working distance objectives, air / water immersion / oil immersion objectives, total internal reflection objectives, and super-resolution structured light compatible objectives, depending on the excitation mode. This supports focusing of the incident excitation light and high-sensitivity capture of the emission signal. The upright three-dimensional sample manipulation platform supports XYZ three-axis electric or piezoelectric displacement control and has high-precision scanning and positioning capabilities. This module is designed to support single-beam or dual-beam excitation configurations and can be flexibly connected to image acquisition, spectral analysis or time response subsystems. It is suitable for luminescent systems of various samples such as thin film solid materials, droplets, microscopic slides, microfluidic chips, biological cells, and tissue sections.

[0012] In the single / dual-beam laser-side excitation mode and the single-beam LED-side excitation mode, the sample-side excitation module includes a first reflector, a second reflector, a sample dynamic stirring unit, and a lateral excitation sample carrying platform. The excitation light from the multi-modal function configuration module / second laser beam modulation module, after being incident on the sample, passes through the first and second reflectors to laterally excite the sample in the sample carrying platform. The sample then enters the multi-modal function configuration module via the same first reflector and is directed to imaging acquisition, spectral resolution, and time-domain response modules according to experimental requirements. The sample dynamic stirring unit is used to stir the sample in real time, ensuring that the liquid sample remains uniformly distributed or dynamically mixed during detection. The lateral excitation sample carrying platform is a sample-accommodating structure designed to accommodate lateral incident excitation paths, enabling high-throughput detection or in-situ dynamic reaction system characterization. This module design supports single-beam or dual-beam excitation configurations and can be flexibly integrated with image acquisition, spectral analysis, or time-response subsystems, making it suitable for luminescent systems with dynamic changes, such as particulate dispersions, fluorescent solutions, and reaction mixtures.

[0013] In the single-beam laser inverted excitation mode, the sample inverted excitation module includes a third reflecting mirror, an inverted objective lens unit, and an inverted three-dimensional sample manipulation platform. Excitation light from the multi-modal functional configuration module is focused by the inverted objective lens unit via the third reflecting mirror and then introduced from the bottom of the system or obliquely into the upper inverted three-dimensional sample manipulation platform. The inverted objective lens unit is located below the sample and can be equipped with various types of objectives, such as high / low numerical aperture objectives, long / short working distance objectives, air / water immersion / oil immersion objectives, total internal reflection objectives, and super-resolution structured light compatible objectives, depending on the excitation mode. This supports focusing of the incident excitation light and high-sensitivity capture of the emission signal. The inverted three-dimensional sample manipulation platform is used to fix and move the sample position, supporting XYZ axis electric adjustment and micro-precision positioning. This module design supports single-beam excitation configuration and can be flexibly integrated with image acquisition, spectral analysis, or time response subsystems. It is suitable for luminescent systems such as live cell bottom attachment culture, cuvette bottom detection, microfluidic chips, and bottom injection observation.

[0014] The digital image acquisition module includes a fourth wavelength selection component, a first focusing lens, and an imaging sensing unit. The emission signal from the multimodal function configuration module undergoes spectral purification via the fourth wavelength selection component, is further spatially focused by the first focusing lens, and finally coupled into the imaging sensing unit for image acquisition and digital processing. The fourth wavelength selection component preferably employs a bandpass filter, long / short pass filter, variable filter group, or acousto-optic / electro-optic tunable filter system, etc., and the specific configuration can be flexibly adjusted according to the application wavelength. The first focusing lens preferably employs a single lens, compound lens, or adjustable focus lens system, optimized based on the characteristics of the acquired signal. The imaging sensing unit preferably uses a high-sensitivity camera such as a research-grade CMOS, sCMOS, EMCCD, or CCD to adapt to different resolution and sensitivity requirements.

[0015] The spectral resolution acquisition module includes a fifth wavelength selection component, a second focusing mirror, a first optical signal transmission interface, and a spectral analysis unit. The emitted signal from the multimodal function configuration module undergoes spectral purification via the fifth wavelength selection component, is further spatially focused by the second focusing mirror, and is then transmitted to the spectral analysis unit via the first optical signal transmission interface. Preferably, the fifth wavelength selection component employs a bandpass filter, long / short pass filter, variable filter group, or acousto-optic / electro-optic tunable filter system, with the specific configuration adjustable according to the application wavelength. The second focusing mirror preferably employs a single lens, compound lens, or adjustable focus lens system, optimized based on the characteristics of the acquired signal. The first optical signal transmission interface preferably uses a multimode fiber, single-mode fiber, liquid-core optical guide, or free-space coupler structure for efficient transmission of the optical signal to the subsequent analysis device. The spectral analysis unit preferably employs a diffraction grating spectrometer, Fourier transform spectrometer, multichannel spectrometer, or CMOS array spectral sensor to achieve wavelength resolution and intensity acquisition of the emitted signal.

[0016] The time-domain response acquisition module includes a sixth wavelength selection component, a third focusing mirror, a second optical signal transmission interface, and a time-resolved photon detection unit. The emitted signal from the multimodal function configuration module undergoes spectral purification via the sixth wavelength selection component, is further spatially focused by the third focusing mirror, and is transmitted to the time-resolved photon detection unit via the second optical signal transmission interface. This unit is used to acquire the dynamic response characteristics of the emitted signal on a nanosecond to second timescale, including rise time, emission lifetime, and transient dynamic changes. The sixth wavelength selection component preferably employs a bandpass filter, long / short pass filter, variable filter group, acousto-optic / electro-optic tunable filter system, etc., and the specific configuration can be flexibly adjusted according to the application wavelength band; the third focusing lens preferably employs a single lens, compound lens, or adjustable focus lens system, and the selection is made according to the characteristics of the acquired signal; the second optical signal transmission interface preferably employs a multimode fiber, single-mode fiber, liquid-core optical guide, or free-space coupler structure, etc., for efficient transmission of optical signals to the subsequent analysis device; the time-resolved photon detection unit preferably employs an avalanche photodiode (APD), single-photon counting module (SPCM), time-correlated single-photon counting system (TCSPC), high-speed photomultiplier tube (PMT), etc., for achieving high-precision dynamic acquisition of time-domain luminescence response;

[0017] Furthermore, the first and second laser source units preferably employ continuous wave lasers, modulated pulsed lasers, semiconductor lasers, fiber lasers, solid-state lasers, femtosecond / picosecond ultrafast lasers, etc.; the first and second wavelength selection components preferably employ bandpass filters, long / short pass filters, variable filter groups, acousto-optic / electro-optic tunable filter systems, etc.; the first and second beam quality control units preferably employ beam collimators, beam expanders / contractors, spatial filters, beam shapers (such as cylindrical mirrors, DOE diffraction optical elements), etc.; the first and second polarization state control units preferably employ polarizers, waveplates (1 / 4λ, 1 / 2λ), polarization rotators, liquid crystal polarization modulators, or electro-optic polarizers, etc.

[0018] Furthermore, the broadband light source unit preferably employs a high-brightness single-band LED, a multi-band composite LED, a chip-integrated LED array, an organic light-emitting diode (OLED), or a semiconductor narrow-band light-emitting element; the third wavelength selection component preferably employs a bandpass filter, a long / short pass filter, a variable filter group, an acousto-optic / electro-optic tunable filter system, etc.; the third beam quality control unit preferably employs a beam collimator, a beam expander / contractor system, a spatial filter, a beam shaper (such as a cylindrical mirror, a DOE diffractive optical element), etc.

[0019] Furthermore, in the single / dual-beam laser upright excitation mode, the fourth submodule is equipped with a dichroic mirror to reflect the excitation light output from the first laser beam modulation module to the sample upright excitation module, and transmit the emission signal to the digital image / spectral resolution / temporal response acquisition module; the fifth submodule is equipped with a dichroic mirror to reflect the excitation light output from the second laser beam modulation module to the sample upright excitation module, and transmit the emission signal to the digital image / spectral resolution / temporal response acquisition module; the sixth and seventh submodules are both removed.

[0020] Furthermore, in the single / dual-beam laser-side excitation mode, the fourth submodule is equipped with a dichroic mirror to reflect the excitation light output from the first laser beam modulation module to the fifth submodule and transmit the emission signal from the fifth submodule to the digital image / spectral resolution / temporal response acquisition module; the fifth submodule is equipped with a reflector to reflect the excitation light / emission signal from the fourth / sixth submodule; the sixth submodule is equipped with a reflector to reflect the excitation light / emission signal from the fifth submodule / sample-side excitation module; and the seventh submodule is equipped with a reflector to reflect the excitation light output from the second laser beam modulation module to the sample-side excitation module.

[0021] Furthermore, in the single-beam LED upright excitation mode, the fifth submodule is equipped with a dichroic mirror to reflect the excitation light output from the LED beam modulation module to the sample upright excitation module, and transmit the emission signal to the digital image / spectral resolution / time domain response acquisition module; the fourth, sixth, and seventh submodules are all removed.

[0022] Furthermore, in the single-beam LED-side excitation mode, the fifth submodule is equipped with a dichroic mirror to transmit the excitation light output from the LED beam modulation module to the sixth submodule and reflect the emission signal to the digital image / spectral resolution / temporal response acquisition module; the sixth submodule is equipped with a reflector to reflect the excitation light / emission signal from the fifth submodule / sample-side excitation module; the fourth and seventh submodules are both removed.

[0023] Furthermore, in the single-beam laser inverted excitation mode, the fourth submodule is equipped with a dichroic mirror to transmit the excitation light output from the first laser beam modulation module to the sample inverted excitation module and reflect the emission signal to the digital image / spectral resolution / temporal response acquisition module; the fifth, sixth, and seventh submodules are all removed.

[0024] Furthermore, the luminescent materials include, but are not limited to: fluorescent dyes, quantum dots, upconversion nanoparticles, rare earth-doped phosphors, long afterglow materials, perovskite nanocrystals, two-dimensional luminescent materials, carbon-based luminescent materials, metal / covalent-organic frameworks, and other organic / inorganic luminescent materials with excitation-responsive capabilities.

[0025] A highly integrated multimodal fluorescence kinetics testing method, characterized by comprising the following steps:

[0026] S1: Excitation mode selection: Based on the luminescence characteristics of the tested luminescent material, sample morphology, and experimental requirements, the system can be set to one of the following modes through the multi-mode function configuration module: single / dual-beam laser upright excitation mode, single / dual-beam laser side excitation mode, single-beam LED upright excitation mode, single-beam LED side excitation mode, or single-beam laser inverted excitation mode.

[0027] S2: Light Source Modulation Configuration: When laser excitation is selected, the first laser beam modulation module, the first laser source unit, the second laser source unit, the first wavelength selection component, the second wavelength selection component, the first beam quality control unit, the second beam quality control unit, the first polarization state control unit, and the second polarization state control unit are configured sequentially to achieve excitation light output with specific wavelength, polarization, and beam shape; when LED excitation is selected, the broadband source unit, the third wavelength selection component, and the third beam quality control unit in the LED beam modulation module are configured to output excitation light with the required wavelength and shape;

[0028] S3: Sample handling and adaptation: Depending on the sample type and structure, fix the sample on the upright three-dimensional sample handling platform, the inverted three-dimensional sample handling platform, or the lateral excitation sample carrying platform; if dynamic mixing is required, activate the sample dynamic stirring unit to homogenize the sample, ensuring the stability and representativeness of the experiment.

[0029] S4: Optical Path Configuration and Excitation Implementation: The modulated excitation light is configured with the input path of the excitation beam through the sub-modules (sub-modules 4 to 7) in the multimodal function configuration module, and imported into the selected sample excitation module (sample upright excitation module, sample side excitation module, sample inverted excitation module). The light is then focused onto the sample to be tested through the corresponding objective lens / optical path assembly to achieve excitation. The output path of the emission signal is configured with the sub-modules (sub-modules 1 to 3) in the multimodal function configuration module, and the digital image acquisition module, spectral resolution acquisition module, or time domain response acquisition module is selected to be imported to achieve acquisition.

[0030] S5: Signal Processing and Data Output: In the image acquisition path, the emitted light sequentially passes through the fourth wavelength selection component and the first focusing lens, and enters the imaging sensing unit to complete digital image acquisition and processing; in the spectral resolution path, the emitted signal sequentially passes through the fifth wavelength selection component, the second focusing lens, and the first optical signal transmission interface to enter the spectral analysis unit for spectral feature extraction; in the time response path, the emitted signal passes through the sixth wavelength selection component, the third focusing lens, and the second optical signal transmission interface to enter the time-resolved photon detection unit to obtain time-domain dynamic characteristics such as emission lifetime and rise time;

[0031] In step S1, the excitation method required for the experiment is determined by setting the excitation path through a multimodal configuration module based on factors such as the photophysical properties of the luminescent material being tested (e.g., excitation wavelength, excitation type, structural symmetry), the sample support method (e.g., solution, solid film, cells, tissue), and the experimental objective (e.g., imaging, spectral analysis, lifetime determination). Preferably, for attached cells or thin film materials, upright or inverted excitation modes are preferred; for dispersed solutions, nanoparticles, and colloidal systems, lateral excitation modes are preferred; for optical processes requiring multiple excitations, a dual-beam mode is used; and for the above application scenarios, continuous / pulsed / ultrafast light source modes are selected as needed.

[0032] In step S1, the excitation mode is adjusted according to the material's excitation wavelength range, photostability, and excitation efficiency. Preferably, a laser excitation mode is used for narrowband excitation requirements; an LED excitation mode is used for broadband excitation requirements; and a dual-source composite excitation path can be set for multi-band co-excitation requirements.

[0033] In step S2, the laser output power of the first and second laser source units can be adjusted by hardware current regulation, electro-modulation, or optical attenuation, and the wavelength selection range covers the ultraviolet to near-infrared band, suitable for matching the absorption characteristics of different luminescent materials; the first, second, and third wavelength selection components have the ability to quickly switch bands, realizing the individual extraction, composite tuning, and wavelength switching of multi-band laser sources; the first, second, and third beam quality control units realize the size control of the laser spot, energy uniformity optimization, spatial coherence, and coupling adjustment, which can further enhance the irradiation efficiency of the light source and the consistency of sample excitation; the first and second polarization state control units realize the control between linear polarization, circular polarization, and elliptic polarization, supporting the response control of the excitation polarization state to anisotropic luminescent materials, as well as polarization-dependent luminescence measurement;

[0034] In step S3, the upright three-dimensional sample manipulation platform supports XYZ three-axis electric displacement and micro / nano-level precision fine-tuning, and is compatible with various carrier structures such as microscope slides, culture dishes, multi-well plates, thin film materials, and organ fragments. Optional components include sample clamps, sample platform adapters, positioning frames, and multi-well supports. It can be further integrated with an electric focusing module and a temperature control module to achieve long-term stable observation. The inverted three-dimensional sample manipulation platform supports XYZ three-axis electric displacement and micro / nano-level precision fine-tuning, and is compatible with various container structures, including bottom-attached cell culture dishes, transparent bottom reaction dishes, etc. The system includes cuvettes, microfluidic chip bottom detection, and glass-bottomed porous plates, and can integrate auxiliary functions such as temperature control modules, gas control modules, and biocompatible fixtures according to experimental needs. The lateral excitation sample carrying platform supports the fixation of cuvettes, transparent sample vials, glass reaction chambers, flow cells, and closed liquid microcavities, and is compatible with sample containment structures designed for lateral incident excitation paths, enabling high-throughput detection or in-situ dynamic reaction system characterization. The sample dynamic stirring unit can be implemented through magnetic stirring structures, electromagnetic oscillators, ultrasonic exciters, and microfluidic driven pumps, enabling dynamic system detection under in-situ observation. The magnetic stirring structure is compatible with conventional cuvettes and liquid reaction flasks, and can be used with room-temperature magnetic stirrers; electromagnetic oscillators or ultrasonic exciters are used for rapid homogenization of nano-dispersions and colloidal systems; the microfluidic driven pump is suitable for liquid mixing and dynamic loading in microfluidic channels.

[0035] In step S4, the multimodal function configuration module includes sub-modules (first to seventh sub-modules) containing multiple optical path deflection components, preferably motorized rotating mirrors, acousto-optic switches, fiber optic switchers, programmable deflection prisms, manual optical slider structures, rotating apertures, or optical switch arrays. The combined configuration of these sub-modules controls the incident path direction, angle, and beam splitting state of the excitation light, supporting dynamic switching of the excitation light between multiple incident directions (upright, lateral, inverted) to adapt to different excitation modes.

[0036] In step S5, the imaging sensing unit supports advanced imaging functions such as multi-frame continuous acquisition (time-series images), region imaging (ROI), and HDR acquisition; the spectral analysis unit supports common functions such as spectral intensity normalization, peak position identification, spectral smoothing, background subtraction, multi-peak fitting, and component separation; the time-resolved photon detection unit is preferably down to the nanosecond level and supports emission rise time testing, lifetime measurement, energy transfer monitoring, long afterglow detection, TADF / phosphorescence dynamics research, etc., and supports common functions such as signal background removal and lifetime distribution fitting.

[0037] Compared with the prior art, the technical effects and advantages of the present invention are as follows:

[0038] 1. Multi-modal excitation path switching design to improve adaptability: The system integrates upright excitation, lateral excitation and inverted excitation paths, and realizes dynamic optical path reconstruction through adjustable sub-modules (reflectors, dichroic mirrors, etc.), which can adapt to a variety of experimental samples and excitation requirements, including liquid samples, solid films, attached cells, biological tissues, etc.

[0039] 2. Supports multi-source excitation of laser / LED, with high degree of freedom in spectral control; the system is compatible with both laser and LED excitation sources, and is equipped with multi-band selection components and beam quality control modules, enabling complex optical control modes such as multi-wavelength excitation, dual-beam superposition excitation, and polarization state control, thereby improving the excitation efficiency and specificity analysis capabilities of luminescent materials.

[0040] 3. Three-channel linkage acquisition enables multi-dimensional fusion of optical information; This system simultaneously acquires emission signals in three dimensions: imaging, spectrum, and lifetime, enabling the coordinated acquisition of multiple information such as image spatial distribution, emission spectrum characteristics, and emission dynamics, which greatly improves the completeness, analysis depth, and research efficiency of experimental data.

[0041] 4. Modular integrated platform with compact structure and strong scalability: The system adopts a unified optical platform and control module architecture, with interoperability and high integration of functional components. It can be used as a benchtop system or developed into a portable modular platform, facilitating future expansion into a high-throughput detection system or an intelligent automatic acquisition platform. Compared with traditional split-type spectrometers, lifetime meters, microscopes, and other equipment, this system has a more compact structure, and the optical paths between modules do not require complex alignment, significantly improving system stability, reducing experimental errors, and increasing experimental efficiency.

[0042] 5. Significantly reduced costs, enhancing scientific research accessibility and industrialization potential: This system uses modular standard optical components (such as mirrors, filters, focusing lenses, etc.) to build its core architecture. The core components have a high localization rate, replacing expensive imported lifespan meters / fluorescence imaging platforms and other equipment. While ensuring complete functionality, it significantly reduces the overall cost of the machine, making it suitable for promotion to small and medium-sized research institutions, teaching units, and enterprise R&D platforms.

[0043] 6. Adaptable to the detection of various types of luminescent materials: The system is compatible with a variety of typical and cutting-edge luminescent materials, including typical fluorescent materials such as organic small molecule fluorescent dyes, quantum dots, and rare earth-doped luminescent particles; typical phosphorescent materials such as long afterglow materials, TADF systems, and phosphors; and emerging luminescent materials such as perovskite luminescent materials, carbon quantum dots, two-dimensional materials, and MOF / COF photosensitive structures. It has strong adaptability and evolution potential for the evaluation and application research of next-generation luminescent materials. Attached Figure Description

[0044] Figure 1This is a schematic diagram of the optical path structure of a highly integrated multimodal fluorescence dynamics testing system according to Embodiment 1 of the present invention.

[0045] Figure 2 This is a schematic diagram of the optical path structure of the single / dual-beam laser upright excitation mode in Embodiment 1 of the present invention. Figures a and c are schematic diagrams of the optical path structure for digital image acquisition, spectral resolution acquisition, and time-domain response acquisition functions under the single / dual-beam laser upright excitation mode, respectively. Figure d is a schematic diagram of the light transmission mode corresponding to the optical path deflection component of the sub-module within the multi-modal function configuration module in the optical path structure of the digital image acquisition, spectral resolution acquisition, and time-domain response acquisition functions.

[0046] Figure 3 This is a schematic diagram of the optical path structure of the single / dual-beam laser-excited mode in Embodiment 1 of the present invention. Figures a and c are schematic diagrams of the optical path structure for digital image acquisition, spectral resolution acquisition, and time-domain response acquisition functions under the single / dual-beam laser upright excitation mode, respectively. Figure d is a schematic diagram of the light transmission mode corresponding to the optical path deflection component of the sub-module within the multi-modal function configuration module in the optical path structure of the digital image acquisition, spectral resolution acquisition, and time-domain response acquisition functions.

[0047] Figure 4 This is a schematic diagram of the optical path structure of the single-beam LED upright excitation mode in Embodiment 1 of the present invention. Figures a and c are schematic diagrams of the optical path structures for digital image acquisition, spectral resolution acquisition, and time-domain response acquisition functions under single / dual-beam laser upright excitation modes, respectively. Figure d is a schematic diagram of the light transmission mode corresponding to the optical path deflection component of the sub-module within the multi-modal function configuration module in the optical path structure of the digital image acquisition, spectral resolution acquisition, and time-domain response acquisition functions.

[0048] Figure 5 This is a schematic diagram of the optical path structure of the single-beam LED-side excitation mode in Embodiment 1 of the present invention. Figures a and c are schematic diagrams of the optical path structure for digital image acquisition, spectral resolution acquisition, and time-domain response acquisition functions under single / dual-beam laser upright excitation modes, respectively. Figure d is a schematic diagram of the light transmission mode corresponding to the optical path deflection component of the sub-module within the multi-modal function configuration module in the optical path structure for digital image acquisition, spectral resolution acquisition, and time-domain response acquisition functions.

[0049] Figure 6 This is a schematic diagram of the optical path structure of the single-beam laser inverted excitation mode in Embodiment 1 of the present invention. Figures a and c are schematic diagrams of the optical path structures for digital image acquisition, spectral resolution acquisition, and time-domain response acquisition functions under single / dual-beam laser upright excitation modes, respectively. Figure d is a schematic diagram of the light transmission mode corresponding to the optical path deflection component of the sub-module within the multi-modal function configuration module in the optical path structure of the digital image acquisition, spectral resolution acquisition, and time-domain response acquisition functions.

[0050] Figure 7 Figure 3 shows the spectral curves under single / dual-beam laser upright excitation modes in Embodiment 3 of the present invention. Specifically, Figure a shows the visible light spectrum of a glass slide based on the NaYF4:Nd / Yb / Tm luminescent system under 808 nm continuous laser excitation; Figure b shows the visible light spectrum of a glass slide based on the NaYF4:Yb / Er luminescent system under 980 nm continuous laser excitation; and Figure c shows the visible light spectrum of a mixed system of NaYF4:Yb / Er and NaYF4:Nd / Yb / Tm under 808 nm & 980 nm dual continuous laser excitation.

[0051] Figure 8 The results of fluorescence dynamics analysis under the single-beam laser inverted excitation mode in Example 4 of this invention are shown. Figure a shows the near-infrared spectrum of the glass slide based on the NaYF4:Tm luminescent system under 808 nm continuous laser excitation; Figure b shows the fluorescence rise time test and fitting curve of this system; Figure c shows the fluorescence lifetime test and fitting curve of this system.

[0052] Figure 9 The results of fluorescence kinetic analysis under the single-beam laser-excited mode in Example 5 of this invention are shown. Figure a shows the visible light spectrum of the NaYF4:Er@NaYF4 luminescent system under 1532 nm continuous laser excitation; Figure b shows the fluorescence rise time / lifetime test and fitting curve for the 650 nm emission peak; Figure c shows the fluorescence rise time / lifetime test and fitting curve for the 810 nm emission peak; and Figure d shows the fluorescence rise time / lifetime test and fitting curve for the 980 nm emission peak.

[0053] Figure 10 The dynamic luminescence variation spectrum curves under the single-beam laser-side excitation mode in Embodiment 6 of the present invention show that the luminescence of the NaGdF4:Yb / Er system based on pyridine-2-carboxylic acid modification gradually increases with the increase of stirring time.

[0054] Figure 11 This is a schematic diagram of camera imaging under the single-beam laser upright excitation mode in Embodiment 7 of the present invention. Figure a shows the camera imaging result of the NaYF4:Er luminescent system under bright field conditions; Figure b shows the camera imaging result of the NaYF4:Er luminescent system under 980 nm laser excitation.

[0055] exist Figures 1-6 In the middle, each is marked as:

[0056] 1. First laser beam modulation module; 2. Second laser beam modulation module; 3. LED beam modulation module; 4. Multimodal function configuration module; 5. Sample upright excitation module; 6. Sample side excitation module; 7. Sample inverted excitation module; 8. Digital image acquisition module; 9. Spectral resolution acquisition module; 10. Time domain response acquisition module;

[0057] 11. First laser source unit; 12. First wavelength selection component; 13. First beam quality control unit; 14. First polarization state control unit;

[0058] 21. Second laser source unit; 22. Second wavelength selection component; 23. Second beam quality control unit; 24. Second polarization state control unit;

[0059] 31. Broadband light source unit; 32. Third wavelength selection component; 33. Third beam quality control unit;

[0060] 41. First submodule; 42. Second submodule; 43. Third submodule; 44. Fourth submodule; 45. Fifth submodule; 46. Sixth submodule; 47. Seventh submodule;

[0061] 51. Upright objective lens unit; 52. Upright three-dimensional sample manipulation platform;

[0062] 61. First reflecting mirror; 62. Sample dynamic stirring unit; 63. Lateral excitation sample carrying platform; 64. Second reflecting mirror;

[0063] 71. Third reflecting mirror; 72. Inverted objective lens unit; 73. Inverted three-dimensional sample manipulation platform;

[0064] 81. Fourth wavelength selection component; 82. First focusing lens; 83. Imaging sensing unit;

[0065] 91. Fifth wavelength selection component; 92. Second focusing lens; 93. First optical signal transmission interface; 94. Spectral analysis unit;

[0066] 101. Sixth wavelength selection component; 102. Third focusing lens; 103. Second optical signal transmission interface; 104. Time-resolved photon detection unit. Detailed Implementation

[0067] The present invention will be further described below. It should be noted that the following embodiments are based on the present technical solution and provide detailed implementation methods and specific operating procedures, but the scope of protection of the present invention is not limited to the following embodiments. In the accompanying drawings, the same or similar reference numerals are used to denote the same or similar elements or elements having the same or similar functions. The embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0068] Example 1

[0069] like Figure 1 As shown, the highly integrated multimodal fluorescence dynamics testing system provided in this embodiment of the invention includes: a first laser beam modulation module 1, a second laser beam modulation module 2, an LED beam modulation module 3, a multimodal function configuration module 4, a sample upright excitation module 5, a sample side excitation module 6, a sample inverted excitation module 7, a digital image acquisition module 8, a spectral resolution acquisition module 9, and a time-domain response acquisition module 10. By switching the usage status of each sub-module of the multimodal function configuration module, digital image, spectral resolution, and time-domain response acquisition can be performed in any of the following modes: single / dual-beam laser upright excitation mode, single / dual-beam laser side excitation mode, single-beam LED upright excitation mode, single-beam LED side excitation mode, and single-beam laser inverted excitation mode.

[0070] Figure 2 (a)-(c) show the optical path structure of the single / dual-beam laser upright excitation mode, respectively configured with digital image, spectral resolution and time domain response acquisition functions; Figure 2(d) in the diagram represents the optical path structure of the digital image acquisition, spectral resolution acquisition, and time-domain response acquisition functions in this mode, showing the light transmission method corresponding to the optical path deflection component of the sub-module within the multi-mode function configuration module. The first laser beam modulation module 1 and the second laser beam modulation module 2 include a near-infrared continuous / pulse tunable laser (first laser source unit 11, second laser source unit 21), and tunable filter groups (first wavelength selection component 12, second wavelength selection component 22), an achromatic beam expander lens group (first beam quality control unit 13, second beam quality control unit 23), and a polarization state control group consisting of a polarizer + half-wave plate + quarter-wave plate (first polarization state control unit 14, second polarization state control unit 24), arranged sequentially along the direction of the laser beam emitted by the laser. The laser with stable output power is purified by the filters and spatially corrected by the lens group. The polarization state control group can output any polarization state (linear polarization, circular polarization, elliptical polarization) into the multi-mode function configuration module 4. The sixth submodule 46 and the seventh submodule 47 are removed. The fourth submodule 44 and the fifth submodule 45 are equipped with dichroic mirrors to reflect the modulated beam output to the sample upright excitation module 5. After being focused by the imaging objective (upright objective lens unit 51), the fluorescent sample held on the upright three-dimensional sample manipulation platform 52 is uprightly excited. The emitted fluorescence is collected by the same imaging objective (upright objective lens unit 51), transmitted through the fourth submodule 44 and the fifth submodule 45, and reflected by the mirrors configured in the third submodule 43, the second submodule 42 and the first submodule 41, respectively, and enters the digital image acquisition module 8, the spectral resolution acquisition module 9 and the time domain response acquisition module 10. The transmitted signal from the input digital image acquisition module 8 is wavelength-selected by the adjustable filter group (fourth wavelength selection component 81), focused by the achromatic focusing lens (first focusing lens 82), and then input into the CCD high-sensitivity camera to complete image acquisition and digital processing. The transmitted signals from the input spectral resolution acquisition module 9 and time-domain response acquisition module 10 are wavelength-selected by the adjustable filter group (fifth wavelength selection component 91, sixth wavelength selection component 101), focused by the achromatic focusing lens (second focusing lens 92, third focusing lens 102), and then coupled into the multimode fiber (first optical signal transmission interface 93, second optical signal transmission interface 103), and input to the spectrometer (spectral analysis unit 94) and the single-photon counter (time-resolved photon detection unit 104) to complete the wavelength resolution, intensity acquisition, and time-domain luminescence response acquisition of the transmitted signal.

[0071] Figure 3 (a)-(c) show the optical path structure of the single / dual-beam laser side excitation mode, respectively configured with digital image, spectral resolution and time domain response acquisition functions; Figure 3(d) in the diagram represents the optical path structure of the digital image acquisition, spectral resolution acquisition, and time-domain response acquisition functions in this mode, showing the light transmission method corresponding to the optical path deflection component of the sub-module within the multi-mode function configuration module. The first laser beam modulation module 1 and the second laser beam modulation module 2 include a near-infrared continuous / pulse tunable laser (first laser source unit 11, second laser source unit 21), and tunable filter groups (first wavelength selection component 12, second wavelength selection component 22), an achromatic beam expander lens group (first beam quality control unit 13, second beam quality control unit 23), and a polarization state control group consisting of a polarizer + half-wave plate + quarter-wave plate (first polarization state control unit 14, second polarization state control unit 24), arranged sequentially along the direction of the laser beam emitted by the laser. The laser with stable output power is purified by the filters and spatially corrected by the lens group. The polarization state control group can output any polarization state (linear polarization, circular polarization, elliptical polarization) into the multi-mode function configuration module 4. The fourth submodule 44 is equipped with a dichroic mirror, and the fifth to seventh submodules 47 are equipped with reflectors. The output modulated beam is excited by the fourth to seventh submodules 44 to 47, the first reflector 61, and the second reflector 64 in a cuvette fixed in a lateral excitation sample support platform 63. A magnetic stirrer (sample dynamic stirring unit 62) is provided at the bottom. The emitted fluorescence is reflected / transmitted by the first reflector 61 and the submodules (fourth to seventh submodules 47), and then reflected by the reflectors configured in the third submodule 43, the second submodule 42, and the first submodule 41, respectively, and enters the digital image acquisition module 8, the spectral resolution acquisition module 9, and the time domain response acquisition module 10. The transmitted signal from the input digital image acquisition module 8 is wavelength-selected by the adjustable filter group (fourth wavelength selection component 81), focused by the achromatic focusing lens (first focusing lens 82), and then input into the CCD high-sensitivity camera to complete image acquisition and digital processing. The transmitted signals from the input spectral resolution acquisition module 9 and time-domain response acquisition module 10 are wavelength-selected by the adjustable filter group (fifth wavelength selection component 91, sixth wavelength selection component 101), focused by the achromatic focusing lens (second focusing lens 92, third focusing lens 102), and then coupled into the multimode fiber (first optical signal transmission interface 93, second optical signal transmission interface 103), and input to the spectrometer (spectral analysis unit 94) and the single-photon counter (time-resolved photon detection unit 104) to complete the wavelength resolution, intensity acquisition, and time-domain luminescence response acquisition of the transmitted signal.

[0072] Figure 4 (a)-(c) show the optical path structure of the single-beam LED upright excitation mode, respectively configured with digital image, spectral resolution and time domain response acquisition functions; Figure 4(d) in the diagram represents the optical path structure of the digital image acquisition, spectral resolution acquisition, and time-domain response acquisition functions in this mode, and is a schematic diagram of the light transmission mode corresponding to the optical path deflection component of the sub-module within the multi-modal function configuration module. The LED beam modulation module 3 includes an LED white light source (broadband light source unit 31), and an adjustable filter group (third wavelength selection component 32) and an achromatic beam expander lens group (third beam quality control unit 33) placed sequentially along the direction of the laser beam emitted by the laser. The white light source outputs stable white light, which enters the multi-modal function configuration module 4 after the filter selects the wavelength and the lens group spatially corrects it. The fourth sub-module 44, the sixth sub-module 46, and the seventh sub-module 47 are removed, and the fifth sub-module 45 is equipped with a dichroic mirror to reflect the output modulated beam to the sample upright excitation module 5. After being focused by the imaging objective (upright objective lens unit 51), the fluorescent sample held on the upright three-dimensional sample manipulation platform 52 is uprightly excited. The emitted fluorescence is collected by the same imaging objective (upright objective unit 51), transmitted through the fifth submodule 45, and reflected by the mirrors configured in the third submodule 43, the second submodule 42 and the first submodule 41, respectively, and enters the digital image acquisition module 8, the spectral resolution acquisition module 9 and the time domain response acquisition module 10. The transmitted signal from the input digital image acquisition module 8 is wavelength-selected by the adjustable filter group (fourth wavelength selection component 81), focused by the achromatic focusing lens (first focusing lens 82), and then input into the CCD high-sensitivity camera to complete image acquisition and digital processing. The transmitted signals from the input spectral resolution acquisition module 9 and time-domain response acquisition module 10 are wavelength-selected by the adjustable filter group (fifth wavelength selection component 91, sixth wavelength selection component 101), focused by the achromatic focusing lens (second focusing lens 92, third focusing lens 102), and then coupled into the multimode fiber (first optical signal transmission interface 93, second optical signal transmission interface 103), and input to the spectrometer (spectral analysis unit 94) and the single-photon counter (time-resolved photon detection unit 104) to complete the wavelength resolution, intensity acquisition, and time-domain luminescence response acquisition of the transmitted signal.

[0073] Figure 5 (a)-(c) show the optical path structure of the single-beam LED side excitation mode, respectively configured with digital image, spectral resolution and time domain response acquisition functions; Figure 5(d) in the diagram represents the optical path structure of the digital image acquisition, spectral resolution acquisition, and time-domain response acquisition functions in this mode, showing the light transmission method corresponding to the optical path deflection component of the sub-module within the multi-modal function configuration module. The LED beam modulation module 3 includes an LED white light source (broadband light source unit 31), and an adjustable filter group (third wavelength selection component 32) and an achromatic beam expander lens group (third beam quality control unit 33) placed sequentially along the direction of the laser beam emitted by the laser. The white light source outputs stable white light through the filter selection band and the lens group spatial correction before entering the multi-modal function configuration module 4. The fourth sub-module 44 and the seventh sub-module 47 are removed, and the fifth sub-module 45 is equipped with a dichroic mirror. The modulated beam transmitted through the mirror configured in the sixth sub-module 46 and the first mirror 61 excites the cuvette sample fixed in the lateral excitation sample support platform 63. A magnetic stirrer (sample dynamic stirring unit 62) is equipped at the bottom. The emitted fluorescence is reflected by the first reflector 61, the sixth submodule 46, and the fifth submodule 45, and then reflected by the reflectors configured in the third submodule 43, the second submodule 42, and the first submodule 41, respectively, and enters the digital image acquisition module 8, the spectral resolution acquisition module 9, and the time domain response acquisition module 10. The transmitted signal from the input digital image acquisition module 8 is wavelength-selected by the adjustable filter group (fourth wavelength selection component 81), focused by the achromatic focusing lens (first focusing lens 82), and then input into the CCD high-sensitivity camera to complete image acquisition and digital processing. The transmitted signals from the input spectral resolution acquisition module 9 and time-domain response acquisition module 10 are wavelength-selected by the adjustable filter group (fifth wavelength selection component 91, sixth wavelength selection component 101), focused by the achromatic focusing lens (second focusing lens 92, third focusing lens 102), and then coupled into the multimode fiber (first optical signal transmission interface 93, second optical signal transmission interface 103), and input to the spectrometer (spectral analysis unit 94) and the single-photon counter (time-resolved photon detection unit 104) to complete the wavelength resolution, intensity acquisition, and time-domain luminescence response acquisition of the transmitted signal.

[0074] Figure 6 (a)-(c) show the optical path structure of the single-beam laser inverted excitation mode, respectively configured with digital image, spectral resolution and time domain response acquisition functions; Figure 6(d) in the diagram represents the optical path structure of the digital image acquisition, spectral resolution acquisition, and time-domain response acquisition functions in this mode, specifically the light transmission method corresponding to the optical path deflection component of the sub-module within the multi-mode function configuration module. The first laser beam modulation module 1 includes a near-infrared continuous / pulse tunable laser (first laser source unit 11), and tunable filter group (first wavelength selection component 12), achromatic beam expander lens group (first beam quality control unit 13), and polarization state control group (first polarization state control unit 14) consisting of a polarizer + half-wave plate + quarter-wave plate, arranged sequentially along the direction of the laser beam emitted by the laser. The laser with stable output power is purified by the filter group and spatially corrected by the lens group. The polarization state control group can output any polarization state (linear polarization, circular polarization, elliptical polarization) into the multi-mode function configuration module 4. Submodules 45 through 47 are removed. Submodule 44 is equipped with a dichroic mirror, transmitting the modulated beam to the inverted sample excitation module 7. After being reflected by the third mirror 71 and focused by the imaging objective (inverted objective unit 72), the fluorescent sample held on the inverted three-dimensional sample manipulation platform 73 is excited. The emitted fluorescence is collected by the same imaging objective (inverted objective unit 72), and after being reflected by the third mirror 71 and the fourth submodule 44, it is reflected by the mirrors configured in the third submodule 43, the second submodule 42, and the first submodule 41, respectively, and enters the digital image acquisition module 8, the spectral resolution acquisition module 9, and the time domain response acquisition module 10. The transmitted signal from the input digital image acquisition module 8 is wavelength-selected by the adjustable filter group (fourth wavelength selection component 81), focused by the achromatic focusing lens (first focusing lens 82), and then input into the CCD high-sensitivity camera to complete image acquisition and digital processing. The transmitted signals from the input spectral resolution acquisition module 9 and time-domain response acquisition module 10 are wavelength-selected by the adjustable filter group (fifth wavelength selection component 91, sixth wavelength selection component 101), focused by the achromatic focusing lens (second focusing lens 92, third focusing lens 102), and then coupled into the multimode fiber (first optical signal transmission interface 93, second optical signal transmission interface 103), and input to the spectrometer (spectral analysis unit 94) and the single-photon counter (time-resolved photon detection unit 104) to complete the wavelength resolution, intensity acquisition, and time-domain luminescence response acquisition of the transmitted signal.

[0075] Example 2

[0076] This embodiment provides a highly integrated multimodal fluorescence dynamics testing method, including the following steps:

[0077] S1: Excitation mode selection: Based on the luminescence characteristics of the tested luminescent material, sample morphology, and experimental requirements, the system can be set to one of the following modes through the multi-mode function configuration module 4: single / dual-beam laser upright excitation mode, single / dual-beam laser side excitation mode, single-beam LED upright excitation mode, single-beam LED side excitation mode, or single-beam laser inverted excitation mode;

[0078] The method for determining the required excitation mode for the experiment involves setting the excitation path through the multimodal configuration module 4, based on factors such as the photophysical properties of the luminescent material being tested (e.g., excitation wavelength, excitation type, structural symmetry), the sample support method (e.g., solution, solid film, cells, tissue), and the experimental objective (e.g., imaging, spectral analysis, lifetime determination). Preferably, for attached cells or thin film materials, an upright or inverted excitation mode is preferred; for dispersed solutions, nanoparticles, and colloidal systems, a lateral excitation mode is preferred; for optical processes requiring multiple excitations, a dual-beam mode is used; and for the above application scenarios, the light source can be selected in continuous / pulsed / ultrafast mode as needed.

[0079] The excitation mode is adjusted according to the material's excitation wavelength range, photostability, and excitation efficiency. Preferably, laser excitation is used for narrowband excitation requirements; LED excitation is used for broadband excitation requirements; and a dual-source composite excitation path can be set for multi-band co-excitation requirements.

[0080] S2: Light source modulation configuration: When laser excitation is selected, the first laser source unit 11, the second laser source unit 21, the first wavelength selection component 12, the second wavelength selection component 22, the first beam quality control unit 13, the second beam quality control unit 23, the first polarization state control unit 14, and the second polarization state control unit 24 in the first laser beam modulation module 1 and the second laser beam modulation module 2 are configured in sequence to achieve excitation light output with specific wavelength, polarization, and beam shape; when LED excitation is selected, the broadband source unit 31, the third wavelength selection component 32, and the third beam quality control unit 33 in the LED beam modulation module 3 are configured to output excitation light with the required wavelength and shape;

[0081] The laser output power of the first laser source unit 11 and the second laser source unit 21 can be adjusted by hardware current regulation, electro-modulation, or optical attenuation. The wavelength selection range covers the ultraviolet to near-infrared band, suitable for matching the absorption characteristics of different luminescent materials. The first wavelength selection component 12, the second wavelength selection component 22, and the third wavelength selection component 32 have the ability to quickly switch bands, realizing the individual extraction, composite tuning, and wavelength switching of multi-band laser sources. The first beam quality control unit 13, the second beam quality control unit 23, and the third beam quality control unit 33 realize the control of laser spot size, energy uniformity optimization, spatial coherence, and coupling adjustment, which can further enhance the irradiation efficiency of the light source and the consistency of sample excitation. The first polarization state control unit 14 and the second polarization state control unit 24 realize the control between linear polarization, circular polarization, and elliptic polarization, supporting the response control of the excitation polarization state to anisotropic luminescent materials, as well as polarization-dependent luminescence measurement.

[0082] S3: Sample handling and adaptation: Depending on the sample type and structure, fix the sample on the upright three-dimensional sample handling platform 52, the inverted three-dimensional sample handling platform 73, or the laterally excited sample carrying platform 63; if dynamic mixing is required, activate the sample dynamic stirring unit 62 to homogenize the sample, ensuring the stability and representativeness of the experiment.

[0083] The upright three-dimensional sample manipulation platform 52 supports XYZ three-axis electric displacement and micro / nano-level precision fine-tuning, and is compatible with various carrier structures such as microscope slides, culture dishes, multi-well plates, thin film materials, and organ fragments. Optional components include sample clamps, sample platform adapters, positioning frames, and porous supports. It can be further integrated with electric focusing modules and temperature control modules to achieve long-term stable observation. The inverted three-dimensional sample manipulation platform 73 supports XYZ three-axis electric displacement and micro / nano-level precision fine-tuning, and is compatible with various container structures, including bottom-attached cell culture dishes, transparent bottom reaction dishes, cuvettes, microfluidic chip bottom detection, and glass-bottomed multi-well plates. It can also be integrated with auxiliary functions such as temperature control modules, gas control modules, and biocompatible clamps according to experimental needs. The lateral excitation sample carrying platform 63 supports the fixation of cuvettes, transparent sample vials, glass reaction chambers, flow cells, and closed liquid microcavities, and is adapted to sample containment structures designed for lateral incident excitation paths, enabling high-throughput detection or in-situ dynamic reaction system characterization. The sample dynamic stirring unit 62... Dynamic system detection under in-situ observation can be achieved through magnetic stirring structures, electromagnetic oscillators, ultrasonic exciters, and microfluidic driven pumps. Magnetic stirring structures are compatible with conventional cuvettes and liquid reaction flasks, and can be used with room-temperature magnetic stirrers; electromagnetic oscillators or ultrasonic exciters are used for rapid homogenization of nano-dispersions and colloidal systems; microfluidic driven pumps are suitable for liquid mixing and dynamic loading in microfluidic channels.

[0084] S4: Optical Path Configuration and Excitation Implementation: The modulated excitation light is set into the input path of the excitation beam through the fourth sub-module 44 to the seventh sub-module 47 in the multimodal function configuration module 4, and imported into the selected sample upright excitation module 5, sample side excitation module 6, and sample inverted excitation module 7. The light is then focused onto the sample to be tested through the corresponding objective lens / optical path components to achieve excitation. The output path of the emission signal is set through the first sub-module 41 to the third sub-module 43 in the multimodal function configuration module 4, and the digital image acquisition module 8, spectral resolution acquisition module 9, or time domain response acquisition module 10 is selected to be imported to achieve acquisition.

[0085] In the multimodal function configuration module 4, the first submodule 41 to the seventh submodule 47 contain multiple optical path deflection components. In this embodiment, motorized rotating mirrors, acousto-optic switches, fiber optic switchers, programmable deflection prisms, manual optical slider structures, rotating apertures, or optical switch arrays are used. The combined configuration between submodules is used to control the incident path direction, angle, and beam splitting state of the excitation light, supporting dynamic switching of the excitation light between multiple incident directions (upright, lateral, and inverted) to adapt to different excitation modes.

[0086] The digital image acquisition module 8, the spectral resolution acquisition module 9, and the time-domain response acquisition module 10 can selectively import signals through the control system according to the acquisition purpose set in the experiment. The system supports three modes: serial acquisition, parallel synchronous acquisition, and time-series acquisition. In serial acquisition mode, the signal is sequentially acquired through multiple modules, such as for spectral + lifetime joint analysis. In parallel synchronous acquisition mode, the signal is sequentially acquired through multiple modules (for spectral + lifetime joint analysis). In time-series acquisition mode, the acquisition modules are activated by polling according to the experimental settings. Specifically, in this embodiment:

[0087] Serial acquisition: By switching the positions of the reflectors / dichroic mirrors before submodules 41-43, the signals are sequentially entered into different acquisition modules.

[0088] Parallel synchronous acquisition: Before the signal enters submodules 41-43, a beam splitter (such as a three-way beam splitter cube or fiber beam splitter) is set up to distribute the signal to the three paths simultaneously in proportion.

[0089] Time series acquisition: High-speed optical switches (such as acousto-optic tunable filters (AOTF) or electro-optic modulators (EOM)) are used to quickly switch the signal path to different acquisition modules. It should be noted whether the system is equipped with such high-speed switching devices.

[0090] S5: Signal Processing and Data Output: In the image acquisition path, the emitted light sequentially passes through the fourth wavelength selection component 81 and the first focusing lens 82, and enters the imaging sensing unit 83 to complete digital image acquisition and processing; in the spectral resolution path, the emitted signal sequentially passes through the fifth wavelength selection component 91, the second focusing lens 92, and the first optical signal transmission interface 93 to enter the spectral analysis unit 94 for spectral feature extraction; in the time response path, the emitted signal passes through the sixth wavelength selection component 101, the third focusing lens 102, and the second optical signal transmission interface 103 to enter the time-resolved photon detection unit 104 to obtain time-domain dynamic characteristics such as emission lifetime and rise time;

[0091] The imaging sensing unit 83 supports advanced imaging functions such as multi-frame continuous acquisition (time-series images), region of interest (ROI) imaging, and HDR acquisition; the spectral analysis unit 94 supports common functions such as spectral intensity normalization, peak identification, spectral smoothing, background subtraction, multi-peak fitting, and component separation; the time-resolved photon detection unit 104 is preferably down to the nanosecond level and supports emission rise time testing, lifetime measurement, energy transfer monitoring, long afterglow detection, TADF / phosphorescence dynamics research, and other common functions such as signal background removal and lifetime distribution fitting.

[0092] Example 3

[0093] Rare earth-doped upconversion nanoparticles with compositions of NaYF4@NaYF4:Yb / Tm / Nd(90 / 4 / 1%)@NaYF4:Yb / Nd(80 / 20%) and NaYF4:Yb / Er(18 / 2%) were prepared as either independent thin film powder samples or mixed samples. The optical path deflection component of the submodule in the multimodal function configuration module 4 was adjusted to switch to the single / dual-beam laser upright excitation mode. Spectra were acquired using a visible light spectrometer under independent / co-excitation by continuous near-infrared lasers at 808 nm and 980 nm. Figure 7 (a) shows the main emission peaks (455 / 475 nm) of the NaYF4@NaYF4:Yb / Tm / Nd(90 / 4 / 1 %)@NaYF4:Yb / Nd(80 / 20 %) luminescent system under 808 nm continuous laser excitation. The sharp peak positions at 455 nm and 475 nm indicate that the system can efficiently achieve upconversion luminescence in the ultraviolet-visible band under near-infrared excitation, verifying the ability of single-beam laser inverted excitation mode to excite and collect spectra of specific rare earth doped systems. Figure 7(b) shows the main emission peaks (545 / 655 nm) of the NaYF4:Yb / Er(18 / 2%) luminescent system under 980 nm continuous laser excitation. The peak positions of 545 nm (green light) and 655 nm (red light) correspond to the characteristic energy level transitions of Er³⁺ ions, indicating that laser excitation and spectral acquisition under this mode can accurately capture the luminescent characteristics of different rare earth ions, and the spectral signal has a high signal-to-noise ratio and complete peak shape. Figure 7 (c) shows the above-mentioned hybrid luminescent system. Under the co-excitation of continuous near-infrared lasers at 808 nm and 980 nm, the main emission peaks exhibit a distinctive hybrid spectrum. The peak positions are exactly the superposition of 455 nm and 475 nm in (a) and 545 nm and 655 nm in (b), with no obvious peak position shift or signal interference. This directly proves that the dual-beam laser inverted excitation mode can realize the spectral collaborative acquisition under the co-excitation of multiple systems, and verifies the system's compatibility with multi-source composite excitation scenarios and the accuracy of its spectral resolution.

[0094] Experiments show that the present invention exhibits superior excitation specificity, spectral resolution, and multi-source co-excitation compatibility for different rare-earth-doped upconversion materials under single / dual-beam laser inverted excitation modes.

[0095] Example 4

[0096] Rare earth-doped upconversion nanoparticles with a composition of NaYF4:Tm(15%) were used to prepare thin film powder samples. The optical path deflection component of the submodule 4 of the multimodal function configuration module was adjusted to switch to the single-beam laser inverted excitation mode. Spectroscopy was acquired using a near-infrared spectrometer under 808nm near-infrared laser excitation. Figure 8 (a) shows the main emission peaks (1450 / 1650 nm) of the NaYF4:Tm(15%) luminescent system in the 1200–1700 nm range. The distinct peak positions at 1450 nm and 1650 nm verify that the system can produce upconversion luminescence in the near-infrared band under near-infrared excitation, demonstrating the system's high sensitivity in acquiring near-infrared emission signals under single-beam laser inverted excitation mode, and the spectral resolution is sufficient to distinguish characteristic peaks in adjacent bands. Time-domain response analysis was performed on the 1650 nm emission peak by switching filters. Figure 8 (b) and Figure 8 (c) shows the fluorescence rise curve and lifetime decay curve of NaYF4:Tm(15%) nanoparticles, respectively. Figure 8 (b) shows a slow fluorescence rise curve, with a fitted fluorescence rise time of 510 μs, indicating that Tm in this system... 3+The transition process of ions from the ground state to the luminescence energy level has dynamic characteristics of energy transfer or intermediate state accumulation, indicating that the system's time-domain response acquisition module can accurately capture the rising dynamics of the luminescence signal. Figure 8 (c) shows a rapid fluorescence decay curve of the 1650 nm emission peak, with a fitted fluorescence lifetime of 96 μs, illustrating its fluorescence kinetics; corresponding to Tm 3+ The radiative transition process of the ion excited state indicates that the system can effectively obtain the decay kinetics information of short-lived fluorescence, and the time resolution is sufficient to distinguish the different kinetic stages of rise and decay.

[0097] Experiments show that the system of this invention, under single-beam laser inverted excitation mode, possesses excellent near-infrared spectral resolution and precise characterization of time-domain dynamics (rise time and lifetime) of rare-earth-doped upconversion materials, providing a basis for in-depth research on Tm. 3+ The energy level transition mechanism of ions and the luminescence dynamics process provide experimental support.

[0098] Example 5

[0099] Rare-earth-doped upconversion nanoparticles with a composition of NaYF4:Er(70%)@NaYF4 were placed in a standard quartz cuvette. The optical path deflection component of the submodule in the multimodal function configuration module 4 was adjusted to switch to the single-beam laser-side excitation mode. Spectra were acquired using a visible light spectrometer under 1532 nm near-infrared laser excitation. Figure 9 Image (a) shows the main emission peaks (650 / 810 / 980 nm) of the NaYF4:Er(70%)@NaYF4 luminescent system in the 400–1100 nm range. The three characteristic peaks at 650 nm, 810 nm, and 980 nm are clearly defined, with no obvious baseline drift or clutter interference, verifying the efficient excitation capability of the single-beam laser-side excitation mode for liquid samples (held in a quartz cuvette) and the high-resolution acquisition performance of the visible light spectrometer. Time-domain response analysis was performed on the 650 / 810 / 980 nm emission peaks by switching filters. Figure 9 (b) Figure 9 (c) and Figure 9 Figure (d) shows the fluorescence rise curves and lifetime decay curves of NaYF4:Er(70%)@NaYF4 nanoparticles at 650 / 810 / 980 nm, respectively. Figure 9 In (b), the fluorescence rise time and lifetime of the fitted 650 nm emission peak are 3.5 ms and 1.2 ms, respectively; Figure 9 The fluorescence rise time and lifetime of the fitted 810 nm emission peak in (c) are 3.3 ms and 1.1 ms, respectively. Figure 9The fluorescence rise time and lifetime of the (d) fitted 980 nm emission peak were 3.1 ms and 0.7 ms, respectively.

[0100] The experiment verified the applicability of the single-beam laser side-excitation mode to liquid dispersion systems, as well as the spectral resolution and temporal response analysis accuracy of the system in multi-wavelength emission scenarios.

[0101] Example 6

[0102] Rare earth-doped upconversion nanoparticles with a composition of NaGdF4:Yb / Er (18 / 2%) were placed in a standard sample vial and modified with pyridine-2-carboxylic acid ligands. The optical path deflection component of the submodule 4 of the multimodal functional configuration module was switched to the single-beam laser side excitation mode, and a magnetic stirrer was added. The magnetic stirring device was then turned on to thoroughly stir the sample to be tested. Figure 10 This paper presents the dynamic luminescence spectrum of NaGdF4:Yb / Er(18 / 2%) rare-earth-doped upconversion nanoparticles under single-beam laser-side excitation mode, showing the changes in nanoparticle luminescence over stirring time. Under different stirring durations (0 h, 0.5 h, 1 h), the spectral peak positions remained stable, indicating that the single-beam laser-side excitation mode combined with dynamic stirring ensures a stable excitation path and sample environment, and that spectral acquisition is unaffected by stirring, demonstrating strong system compatibility and reliability. With increasing stirring time, the intensity of each characteristic emission peak continuously increases, with the enhancement rate initially rapid and then slowing down, indicating more complete binding of the pyridine-2-carboxylic acid ligand to the upconversion nanoparticles, significantly enhancing the luminescence intensity. The entire spectrum exhibits a stable baseline, clear peak shapes, and no extraneous peaks / noise interference, demonstrating that the single-beam laser-side excitation mode maintains high signal-to-noise ratio spectral acquisition quality even under dynamic stirring conditions.

[0103] Example 7

[0104] Rare earth-doped upconversion microrods with a composition of NaYF4:Yb / Er (18 / 2%) were used to prepare single-particle dispersed samples. The optical path deflection component of the submodule 4 of the multimodal function configuration module was switched to the single-beam laser upright excitation mode, and the acquisition end was adjusted to a scientific research CCD camera. Figure 11 (a) in the image is a bright-field image of the camera with the laser off. Figure 11 Image (b) shows the camera fluorescence image with the laser on, mainly displaying green light and exhibiting good luminescence. The positions of each particle are consistent with bright-field imaging. Figure 1 One-to-one correspondence.

[0105] This invention integrates multimodal testing functions, overcomes the limitations of single-mode detection, improves efficiency and reduces the overall system cost, making the study of luminescence dynamics more efficient, convenient and economical. It is of great value for enhancing my country's research capabilities in life sciences, materials science, nanotechnology and integrated optical systems.

[0106] For those skilled in the art, various corresponding changes and modifications can be made based on the above technical solutions and concepts, and all such changes and modifications should be included within the protection scope of the claims of this invention.

Claims

1. A highly integrated multimodal fluorescence dynamics testing system, characterized in that... include: Light source module: includes at least one of a first laser beam modulation module (1), a second laser beam modulation module (2) and an LED beam modulation module (3), for generating modulated excitation light; Multimodal function configuration module (4): includes multiple configurable optical sub-modules for receiving excitation light from the light source module and switching the excitation light to different excitation paths; Sample excitation module: includes at least one of sample upright excitation module (5), sample side excitation module (6) and sample inverted excitation module (7), for receiving excitation light from the multimodal function configuration module (4) and irradiating the sample, while collecting the emitted light generated by the sample and guiding the emitted light back to the multimodal function configuration module (4). Signal acquisition module: including at least one of digital image acquisition module (8), spectral resolution acquisition module (9) and time domain response acquisition module (10); The multimodal function configuration module (4) can switch the emitted light from the sample excitation module to at least one of the signal acquisition modules; The system dynamically reconstructs the optical path by configuring the optical sub-module in the multimodal function configuration module (4), performs digital image, spectral resolution and time domain response acquisition, and realizes multiple excitation-probe combination modes.

2. The highly integrated multimodal fluorescence dynamics testing system as described in claim 1, characterized in that... By operating the multimodal function configuration module (4), at least one of the following excitation modes can be selectively implemented: Single-beam laser upright excitation mode: including first laser beam modulation module (1), multi-mode function configuration module (4), sample upright excitation module (5), digital image acquisition module (8), spectral resolution acquisition module (9), and time domain response acquisition module (10); Dual-beam laser upright excitation mode: including a first laser beam modulation module (1), a second laser beam modulation module (2), a multi-mode function configuration module (4), a sample upright excitation module (5), a digital image acquisition module (8), a spectral resolution acquisition module (9), and a time domain response acquisition module (10); Single-beam laser-side excitation mode: including first laser beam modulation module (1), multi-mode function configuration module (4), sample-side excitation module (6), digital image acquisition module (8), spectral resolution acquisition module (9), and time domain response acquisition module (10); Dual-beam laser-side excitation mode: including a first laser beam modulation module (1), a second laser beam modulation module (2), a multi-mode function configuration module (4), a sample-side excitation module (6), a digital image acquisition module (8), a spectral resolution acquisition module (9), and a time-domain response acquisition module (10); Single-beam LED upright excitation mode: including LED beam modulation module (3), multi-mode function configuration module (4), sample upright excitation module (5), digital image acquisition module (8), spectral resolution acquisition module (9), and time domain response acquisition module (10); Single-beam LED-side excitation mode: including LED beam modulation module (3), multi-mode function configuration module (4), sample-side excitation module (6), digital image acquisition module (8), spectral resolution acquisition module (9), and time domain response acquisition module (10); Single-beam laser inverted excitation mode: including laser beam modulation module (1), multi-mode function configuration module (4), sample inverted excitation module (7), digital image acquisition module (8), spectral resolution acquisition module (9), and time domain response acquisition module (10); The functions of arbitrary modal camera imaging, emission spectrum and time domain response acquisition are achieved by changing the sub-modules in the multimodal function configuration module (4).

3. The highly integrated multimodal fluorescence dynamics testing system as described in claim 2, characterized in that... In the single-beam laser upright excitation mode, the double-beam laser upright excitation mode, the single-beam laser side excitation mode, the double-beam laser side excitation mode, and the single-beam laser inverted excitation mode, the laser beam modulation module (1) includes a first laser source unit (11), a first wavelength selection component (12), a first beam quality control unit (13), and a first polarization state control unit (14); the first laser source unit (11) generates a continuous / pulsed laser beam output with a wavelength that matches the ground state absorption peak of the sample to be tested, so as to excite a specific energy level transition; the first wavelength selection component (12) is used to purify the laser, and the beam profile, stability, and spatial coherence are optimized by the first beam quality control unit (13); The first polarization state modulation unit (14) modulates the polarization state of the laser to match the optical anisotropy characteristics or polarization selective response of the sample, and finally inputs it into the multi-mode function configuration module (4); the second laser beam modulation module (2) includes a second laser source unit (21), a second wavelength selection component (22), a second beam quality control unit (23) and a second polarization state modulation unit (24); the second laser source unit (21) generates a continuous / pulsed laser beam output with a wavelength that matches the ground state absorption peak of the sample to be tested, so as to excite specific energy level transitions; the second wavelength selection component (22) is used to purify the laser and optimize the beam profile, stability and spatial coherence via the second beam quality control unit (23); The second polarization state modulation unit (24) modulates the polarization state of the laser to match the optical anisotropy characteristics or polarization selective response of the sample, and finally inputs it into the multi-mode function configuration module (4). The first laser source unit (11) and the second laser source unit (21) adopt continuous wave laser, modulated pulse laser, semiconductor laser, fiber laser, solid-state laser, and femtosecond / picosecond ultrafast laser; The first wavelength selection component (12) and the second wavelength selection component (22) employ bandpass filters, long / short pass filters, variable filter groups, and acousto-optic / electro-optic tuned filter systems; the first beam quality control unit (13) and the second beam quality control unit (23) employ beam collimators, beam expanders / contractors, spatial filters, and beam shapers; the first polarization state control unit (14) and the second polarization state control unit (24) employ polarizers, waveplates, polarization rotators, liquid crystal polarization modulators, or electro-optic polarizers.

4. The highly integrated multimodal fluorescence dynamics testing system as described in claim 2, characterized in that... In the single-beam LED upright excitation mode and the single-beam LED side excitation mode, the LED beam modulation module (3) includes a broadband light source unit (31), a third wavelength selection component (32), and a third beam quality control unit (33); the broadband light source unit (31) provides stable and adjustable broadband or narrow spectrum LED output under non-laser conditions; the third wavelength selection component (32) precisely defines the excitation band and avoids interference from non-target wavelengths; The third beam quality control unit (33) shapes the divergent light of the LED into a beam profile, irradiation angle and beam intensity distribution suitable for exciting the sample, and finally inputs it into the multimodal function configuration module (4). The broadband light source unit (31) adopts a high-brightness single-band LED, a multi-band composite LED, a chip-integrated LED array, an organic light-emitting diode, or a semiconductor narrow-spectrum light-emitting element; the third wavelength selection component (32) adopts a bandpass filter, a long / short pass filter, a variable filter group, and an acousto-optic / electro-optic tuned filter system; the third beam quality control unit (33) adopts a beam collimator, a beam expander / contractor system, a spatial filter, and a beam shaper.

5. The highly integrated multimodal fluorescence dynamics testing system as described in claim 2, characterized in that... The multimodal function configuration module (4) contains a first submodule (41) to a seventh submodule (47). The submodules contain optical path deflection components for changing the propagation direction of excitation light and emission signal, and employ dichroic mirrors, reflectors, beam splitters, and electro-optical switches. The third submodule (43) inside the multimodal function configuration module (4) is used to directionally import the emission signal to the digital image acquisition module (8) to capture and digitize the emission image signal of the sample under different excitation modes. The second submodule (42) inside the multimodal function configuration module (4) is used to directionally import the emission signal to the spectral resolution acquisition module (9) to achieve high-sensitivity acquisition of the emission spectrum data of the sample in the excitation state and real-time analysis of the wavelength response characteristics generated during the emission process. The first sub-module (41) inside the multimodal function configuration module (4) is used to directionally import the luminous signal to the time domain response acquisition module (10) to realize the acquisition and analysis of various time characteristics of the luminous rise time and lifetime light signal evolution process over time; In the single-beam laser upright excitation mode and the dual-beam laser upright excitation mode, the fourth sub-module (44) is equipped with a dichroic mirror to reflect the excitation light output by the first laser beam modulation module (1) to the sample upright excitation module (5) and transmit the emission signal to the digital image / spectral resolution / temporal response acquisition module; the fifth sub-module (45) is equipped with a dichroic mirror to reflect the excitation light output by the second laser beam modulation module (2) to the sample upright excitation module (5) and transmit the emission signal to the digital image / spectral resolution / temporal response acquisition module; the sixth sub-module (46) and the seventh sub-module (47) are both removed; In the single-beam laser-side excitation mode and the dual-beam laser-side excitation mode, the fourth submodule (44) is equipped with a dichroic mirror to reflect the excitation light output from the first laser beam modulation module (1) to the fifth submodule (45) and transmit the emission signal from the fifth submodule (45) to the digital image / spectral resolution / temporal response acquisition module; the fifth submodule (45) is equipped with a reflector to reflect the excitation light / emission signal from the fourth submodule (44) / sixth submodule (46); the sixth submodule (46) is equipped with a reflector to reflect the excitation light / emission signal from the fifth submodule (45) / sample-side excitation module (6); the seventh submodule (47) is equipped with a reflector to reflect the excitation light output from the second laser beam modulation module (2) to the sample-side excitation module (6). In the single-beam LED upright excitation mode, the fifth sub-module (45) is equipped with a dichroic mirror to reflect the excitation light output by the LED beam modulation module (3) to the sample upright excitation module (5) and transmit the emission signal to the digital image / spectral resolution / time domain response acquisition module; the fourth sub-module (44), the sixth sub-module (46) and the seventh sub-module (47) are all removed; In the single-beam LED-side excitation mode, the fifth submodule (45) is equipped with a dichroic mirror to transmit the excitation light output from the LED beam modulation module (3) to the sixth submodule (46) and reflect the emission signal to the digital image / spectral resolution / temporal response acquisition module; the sixth submodule (46) is equipped with a reflector to reflect the excitation light / emission signal from the fifth submodule (45) / sample-side excitation module (6); the fourth submodule (44) and the seventh submodule (47) are both removed; In the single-beam laser inverted excitation mode, the fourth sub-module (44) is equipped with a dichroic mirror to transmit the excitation light output by the first laser beam modulation module (1) to the sample inverted excitation module (7) and reflect the emission signal to the digital image / spectral resolution / temporal response acquisition module; the fifth sub-module (45), the sixth sub-module (46) and the seventh sub-module (47) are all removed.

6. The highly integrated multimodal fluorescence dynamics testing system as described in claim 2, characterized in that... In the single / dual-beam laser upright excitation mode and the single-beam LED upright excitation mode, the sample upright excitation module (5) includes an upright objective lens unit (51) and an upright three-dimensional sample manipulation platform (52); the excitation light after passing through the multi-modal function configuration module (4) is focused by the upright objective lens unit (51) to excite the sample held by the upright three-dimensional sample manipulation platform (52), and the emitted light enters the multi-modal function configuration module (4) through the same upright objective lens unit (51), and is directionally imported into the imaging acquisition, spectral resolution, and temporal response function modules according to experimental requirements; the upright objective lens unit (51) is set above the sample, and according to different excitation modes The module is equipped with various types of objectives, including high / low numerical aperture objectives, long / short working distance objectives, air / water immersion / oil immersion objectives, total internal reflection objectives, and super-resolution structured light compatible objectives, supporting the focusing of incident excitation light and high-sensitivity capture of luminescence signals. The upright three-dimensional sample manipulation platform (52) supports XYZ three-axis electric or piezoelectric displacement control and has high-precision scanning and positioning functions. The module is designed to support single-beam or dual-beam excitation configurations and can be flexibly connected to image acquisition, spectral analysis, or time response subsystems. It is suitable for luminescence systems of various samples, including thin film solid materials, droplets, microscopic slides, microfluidic chips, biological cells, and tissue sections. In the single / dual-beam laser-side excitation mode and the single-beam LED-side excitation mode, the sample-side excitation module (6) includes a first reflector (61), a second reflector (64), a sample dynamic stirring unit (62), and a lateral excitation sample carrying platform (63). The excitation light incident from the multi-modal function configuration module (4) / second laser beam modulation module (2) passes through the first reflector (61) / second reflector (64) to laterally excite the sample in the sample carrying platform (63), and enters the multi-modal function configuration module (4) through the same first reflector (61), and is determined according to experimental requirements. The module is designed to import imaging acquisition, spectral resolution, and time-domain response functions; the sample dynamic stirring unit (62) is used to stir the sample in real time, so that the liquid sample is kept uniformly distributed or dynamically mixed during the detection process; the lateral excitation sample carrying platform (63) is a sample containing structure adapted to the lateral incident excitation path design, realizing high-throughput detection or in-situ dynamic reaction system characterization; the module is designed to support single-beam or dual-beam excitation configuration, and can be flexibly connected to image acquisition, spectral analysis or time response subsystems, and is suitable for particulate dispersions, fluorescent solutions, and luminescent systems with dynamic change processes in the reaction mixture; In the single-beam laser inverted excitation mode, the sample inverted excitation module (7) includes a third reflecting mirror (71), an inverted objective lens unit (72), and an inverted three-dimensional sample manipulation platform (73). The excitation light from the multi-modal function configuration module (4) is focused by the inverted objective lens unit (72) via the third reflecting mirror (71) and then introduced from the lower part of the system or obliquely into the upper inverted three-dimensional sample manipulation platform (73). The inverted objective lens unit (72) is located below the sample and can be equipped with various types of objectives, such as high / low numerical aperture objectives, long / short working distance objectives, air / water immersion / oil immersion objectives, total internal reflection objectives, and super-resolution structured light compatible objectives, depending on the excitation mode. This supports the focusing of the incident excitation light and the high-sensitivity capture of the emission signal. The inverted three-dimensional sample manipulation platform (73) is used to fix and move the sample position, and supports electric adjustment and micro-precision positioning of the XYZ axis. The module is designed to support single-beam excitation configuration and can be flexibly connected to image acquisition, spectral analysis or time response subsystems. It is suitable for live cell bottom attachment culture, cuvette bottom detection, microfluidic chip, and bottom injection observation of luminescence system.

7. The highly integrated multimodal fluorescence dynamics testing system as described in claim 1, characterized in that... The digital image acquisition module (8) includes a fourth wavelength selection component (81), a first focusing lens (82), and an imaging sensing unit (83). The emission signal from the multimodal function configuration module (4) is spectrally purified by the fourth wavelength selection component (81), further spatially focused by the first focusing lens (82), and finally coupled into the imaging sensing unit (83) for image acquisition and digital processing. The fourth wavelength selection component (81) uses a bandpass filter, a long / short pass filter, a variable filter group, or an acousto-optic / electro-optic tuned filter system. The specific configuration can be flexibly adjusted according to the application band. The first focusing lens (82) uses a single lens, a compound lens, or a tunable lens system, selected according to the characteristics of the acquired signal. The imaging sensing unit (83) uses a research-grade CMOS, sCMOS, EMCCD, or CCD high-sensitivity camera to adapt to different resolution and sensitivity requirements. The spectral resolution acquisition module (9) includes a fifth wavelength selection component (91), a second focusing lens (92), a first optical signal transmission interface (93), and a spectral analysis unit (94). The emission signal from the multimodal function configuration module (4) is spectrally purified by the fifth wavelength selection component (91), further spatially focused by the second focusing lens (92), and transmitted to the spectral analysis unit (94) through the first optical signal transmission interface (93). The fifth wavelength selection component (91) employs a bandpass filter, a long / short pass filter, a variable filter group, and an acoustic... The optical / electro-optical tuned filtering system can be flexibly adjusted according to the application wavelength; the second focusing lens (92) adopts a single lens, compound lens or adjustable focusing lens system, which is selected according to the characteristics of the acquired signal; the first optical signal transmission interface (93) adopts a multimode fiber, single-mode fiber, liquid core optical guide or free space coupler structure, which is used to efficiently transmit optical signals to the subsequent analysis device; the spectral analysis unit (94) adopts a diffraction grating spectrometer, Fourier transform spectrometer, multichannel spectrometer or CMOS array type spectral sensor, which is used to realize wavelength resolution and intensity acquisition of the emitted signal; The time-domain response acquisition module (10) includes a sixth wavelength selection component (101), a third focusing lens (102), a second optical signal transmission interface (103), and a time-resolved photon detection unit (104). The emission signal from the multimodal function configuration module (4) is spectrally purified by the sixth wavelength selection component (101), further spatially focused by the third focusing lens (102), and transmitted to the time-resolved photon detection unit (104) through the second optical signal transmission interface (103) to acquire the dynamic response characteristics of the emission signal in the nanosecond to second timescale, including rise time, emission lifetime, and transient dynamic changes; wherein, the sixth wavelength selection component (101) A bandpass filter, long / short pass filter, variable filter group, and acousto-optic / electro-optic tuned filter system are adopted, and the specific configuration can be flexibly adjusted according to the application band; the third focusing lens (102) adopts a single lens, compound lens or adjustable focus lens system, and is selected according to the characteristics of the acquired signal; the second optical signal transmission interface (103) adopts a multimode fiber, single-mode fiber, liquid core optical guide or free space coupler structure, which is used to efficiently transmit optical signals to the subsequent analysis device; the time-resolved photon detection unit (104) adopts an avalanche photodiode, a single photon counting module, a time-correlated single photon counting system and a high-speed photomultiplier tube, which is used to realize high-precision dynamic acquisition of time-domain luminescence response.

8. The application of the highly integrated multimodal fluorescence dynamics testing system as described in claim 1 in optical imaging and spectral / temporal resolution analysis of various luminescent materials, wherein the luminescent materials include: Fluorescent dyes, quantum dots, upconversion nanoparticles, rare earth-doped phosphors, long afterglow materials, perovskite nanocrystals, two-dimensional luminescent materials, carbon-based luminescent materials, metal / covalent-organic frameworks, and other organic / inorganic luminescent materials with excitation-responsive capabilities.

9. A highly integrated multimodal fluorescence kinetics testing method, characterized in that... Includes the following steps: S1: Excitation mode selection: Based on the luminescence characteristics of the tested luminescent material, sample morphology and experimental requirements, the system is set to one of the following modes through the multi-mode function configuration module (4): single-beam laser upright excitation mode, double-beam laser upright excitation mode, single-beam laser side excitation mode, double-beam laser side excitation mode, single-beam LED upright excitation mode, single-beam LED side excitation mode, and single-beam laser inverted excitation mode; S2: Light source modulation configuration: When laser excitation is selected, the first laser source unit (11), the second laser source unit (21), the first wavelength selection component (12), the second wavelength selection component (22), the first beam quality control unit (13), the second beam quality control unit (23), the first polarization state control unit (14), and the second polarization state control unit (24) in the first laser beam modulation module (1) and the second laser beam modulation module (2) are configured in sequence to realize the excitation light output with specific wavelength, polarization and beam shape; when LED excitation is selected, the broadband source unit (31), the third wavelength selection component (32) and the third beam quality control unit (33) in the LED beam modulation module (3) are configured to output the excitation light with the required wavelength and shape; S3: Sample handling and adaptation: Depending on the sample type and structure, fix the sample on the upright three-dimensional sample handling platform (52), the inverted three-dimensional sample handling platform (73), or the lateral excitation sample carrying platform (63); if dynamic mixing is required, activate the sample dynamic stirring unit (62) to homogenize the sample and ensure the stability and representativeness of the experiment. S4: Optical path configuration and excitation implementation: The above-modulated excitation light is set through the fourth sub-module (44) to the seventh sub-module (47) in the multimodal function configuration module (4) to set the input path of the excitation beam, and imported into the selected sample upright excitation module (5), sample side excitation module (6), and sample inverted excitation module (7), and the light is focused onto the sample to be tested through the corresponding objective lens / optical path component to achieve excitation; the output path of the luminescence signal is set through the first sub-module (41) to the third sub-module (43) in the multimodal function configuration module (4), and the digital image acquisition module (8), spectral resolution acquisition module (9) or time domain response acquisition module (10) is selected to achieve acquisition; S5: Signal processing and data output: In the image acquisition path, the emitted light passes through the fourth wavelength selection component (81) and the first focusing lens (82) in sequence, and enters the imaging sensing unit (83) to complete digital image acquisition and processing; in the spectral resolution path, the emitted light passes through the fifth wavelength selection component (91), the second focusing lens (92), and the first optical signal transmission interface (93) in sequence to enter the spectral analysis unit (94) for spectral feature extraction; in the time response path, the emitted light passes through the sixth wavelength selection component (101), the third focusing lens (102), and the second optical signal transmission interface (103) to enter the time-resolved photon detection unit (104) to obtain the emission lifetime and rise time.

10. The highly integrated multimodal fluorescence kinetics testing method as described in claim 9, characterized in that... In step S1, the excitation mode selection is based on the photophysical properties of the luminescent material being tested, the sample carrying method, and the experimental objective. The excitation path is set through the multimodal function configuration module (4). For attached cells or thin film materials, upright or inverted excitation modes are preferred. For dispersed solutions, nanoparticles, and colloidal systems, lateral excitation modes are preferred. For optical processes requiring multiple excitations, dual-beam mode is used. The above application scenarios select continuous / pulsed / ultrafast light source modes as needed. In step S1, the excitation mode is adjusted according to the excitation wavelength range, photostability, and excitation efficiency of the material; Laser excitation mode is used for narrowband excitation requirements; LED excitation is used for broadband excitation requirements; and a dual-source composite excitation path is set for multi-band co-excitation requirements. In step S2, the laser output power of the first laser source unit (11) and the second laser source unit (21) is adjusted by hardware current regulation, electro-modulation or optical attenuation, and the wavelength selection range covers the ultraviolet to near-infrared band, which is suitable for matching the absorption characteristics of different luminescent materials; the first wavelength selection component (12), the second wavelength selection component (22), and the third wavelength selection component (32) have the ability to quickly switch bands, realize the individual extraction, composite tuning and wavelength switching of multi-band laser sources; the first beam quality control unit (13), the second beam quality control unit (23), and the third beam quality control unit (33) realize the size control of the laser spot, energy uniformity optimization, spatial coherence and coupling adjustment, further enhancing the irradiation efficiency of the light source and the consistency of sample excitation; the first polarization state control unit (14) and the second polarization state control unit (24) realize the control between linear polarization, circular polarization and elliptic polarization, support the response control of the excitation polarization state to anisotropic luminescent materials, and polarization-dependent luminescence measurement; In step S3, the upright three-dimensional sample manipulation platform (52) supports XYZ triaxial electric displacement and micro / nano-level precision fine-tuning, and is compatible with various carrier structures such as microscope slides, culture dishes, multi-well plates, thin film materials, and organ fragments. It can be equipped with sample clamps, sample platform adapters, positioning frames, and multi-well support components, and further integrates electric focusing modules and temperature control modules to achieve long-term stable observation. The inverted three-dimensional sample manipulation platform (73) supports XYZ triaxial electric displacement and micro / nano-level precision fine-tuning, and is compatible with various container structures, including bottom-attached cell culture dishes, transparent bottom reaction dishes, cuvettes, microfluidic chip bottom detection, and glass-bottom multi-well plates. It also integrates temperature control modules, gas control modules, and biological control modules according to experimental requirements. The device is compatible with the auxiliary functions of the clamp; the lateral excitation sample carrying platform (63) supports the fixation of cuvettes, transparent sample bottles, glass reaction chambers, flow cells, and closed liquid microcavities, and is adapted to the sample containment structure designed for lateral incident excitation paths, so as to realize high-throughput detection or in-situ dynamic reaction system characterization; the sample dynamic stirring unit (62) is realized through a magnetic stirring structure, an electromagnetic oscillator, an ultrasonic exciter, and a microfluidic drive pump, so as to detect dynamic systems under in-situ observation; the magnetic stirring structure is compatible with conventional cuvettes and liquid reaction bottles, and can be used with room temperature magnetic balls; the electromagnetic oscillator or ultrasonic exciter is used for rapid homogenization of nano-dispersions and colloidal systems; the microfluidic drive pump is suitable for liquid mixing and dynamic loading in microfluidic channels; In step S4, the multimodal function configuration module (4) includes multiple optical path deflection components in the first sub-module (41) to the seventh sub-module (47), such as motorized rotating mirrors, acousto-optic switches, fiber optic switchers, programmable deflection prisms, manual optical slider structures, rotating apertures, or optical switch arrays. The combination configuration between the sub-modules is used to control the incident path direction, angle, and beam splitting state of the excitation light, support the dynamic switching of the excitation light between multiple incident directions, and adapt to different excitation modes. In step S4, the digital image acquisition module (8), the spectral resolution acquisition module (9), and the time domain response acquisition module (10) selectively import signals through the control system according to the acquisition purpose set in the experiment. The system supports three modes: serial acquisition, parallel synchronous acquisition, and time series acquisition. In the serial acquisition mode, the signal is sequentially separated and acquired by multiple modules for spectral and lifetime joint analysis. In the parallel synchronous acquisition mode, the signal is sequentially separated and acquired by multiple modules for spectral and lifetime joint analysis. In the time series acquisition mode, the acquisition modules are activated by polling according to the experimental settings. In step S5, the imaging sensing unit (83) supports multi-frame continuous acquisition, regional imaging, and HDR acquisition functions; the spectral analysis unit (94) supports spectral intensity normalization, peak position identification, spectrum smoothing, background subtraction, multi-peak fitting, and component separation functions; the time-resolved photon detection unit (104) is down to the nanosecond level and supports emission rise time testing, lifetime measurement, energy transfer monitoring, long afterglow detection, TADF / phosphorescence dynamics research, and supports signal background removal and lifetime distribution fitting functions.

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