A rapid detection device for sperm motility and a detection method thereof

Abstract

The present application relates to biological detection equipment technical field, disclose a kind of rapid detection device and detection method of sperm motility, including base, microscope and incubator, the incubator is provided with incubation tank, the incubator top is provided with fixture, drive assembly and cover plate.The present application is fixed sperm storage bottle by fixture, then sperm storage bottle is immersed into incubation tank using drive assembly, liquefaction can be realized, and drive assembly can drive sperm storage bottle to be intermittently and briefly inclined inside incubation tank, water immersion sensor can be used to monitor sperm at this time Liquefaction is completed, and drive assembly is used again to move sperm storage bottle to outside incubation tank, compared with artificial observation liquefaction degree, the present application is more accurate, and the present application avoids frequently taking sperm storage bottle from incubation tank, ensures the stability of liquefaction environment, and also can take out sperm in the first time after liquefaction, avoid liquefaction time too long or too short, improve the accuracy of subsequent sperm motility detection.

Description

Technical Field

[0001] This invention relates to the field of biological detection equipment technology, specifically to a rapid sperm motility detection device and its detection method. Background Technology

[0002] Sperm motility testing typically employs specialized equipment combined with laboratory methods. Its main purpose is to assess sperm motility, concentration, and morphology. The main steps include sperm collection, liquefaction, and observation. Observation primarily involves manually observing sperm motility using a standard optical microscope combined with a camera, supplemented by image analysis software.

[0003] Current testing procedures typically involve placing sperm in a sealed bottle, then immersing the bottle in a 37°C constant-temperature water bath. Liquefaction usually takes 30-60 minutes. However, determining the degree of liquefaction currently requires manual observation after removing the bottle. This manual observation is highly experience-dependent and prone to misjudgment, leading to the removal of incompletely liquefied sperm for observation, thus reducing the accuracy of the results. Furthermore, frequent manual removal of the bottle to check the liquefaction level is cumbersome and can disrupt the constant temperature environment in the water bath, interfering with the liquefaction process. In addition, for special sperm that are difficult to liquefy, the liquefaction time is even longer. It is difficult for operators to remove the sperm for observation immediately after liquefaction is complete. If the liquefaction time is prolonged, it will reduce sperm motility and further affect the accuracy of the results. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention provides a rapid sperm motility detection device and method, which has advantages such as solving the problems of insufficient or excessive liquefaction time, poor accuracy, and damage to the liquefaction environment caused by human operation in the sperm liquefaction process of existing detection equipment.

[0005] To solve the above-mentioned technical problems, the present invention provides the following technical solution: a rapid sperm motility detection device, comprising a base, a microscope, and an incubator, wherein an incubator is provided with an incubation groove, and a clamp, a drive assembly, and a cover plate are provided above the incubator. The clamp is used to hold a sperm storage bottle, and the cover plate is used to seal the sperm storage bottle. A water immersion sensor is fixedly installed at the inner edge of the cover plate. The drive assembly is installed on one side of the incubator and is used to drive the clamp to move vertically and also to drive the clamp to rotate.

[0006] Before sperm liquefaction, the sperm storage bottle is held in place by clamps and the cap is sealed on the sperm storage bottle;

[0007] During sperm liquefaction, the drive assembly operates, forcing the clamp and sperm storage bottle to move down into the insulated tank for liquefaction. The operation of the drive assembly also causes the clamp to swing intermittently to an inclined state, forcing the sperm at the bottom of the sperm storage bottle to flow towards the cover plate. When the sperm flows to contact the water immersion sensor, the drive assembly operates again, forcing the clamp and sperm storage bottle to move out of the insulated tank.

[0008] Preferably, the clamp includes a vertically arranged ring, and the side wall of the ring is provided with a fastening bolt, which passes through the ring and is threadedly connected to the ring.

[0009] Preferably, the driving assembly includes a vertical slide fixed to the side wall of the insulation box, a vertical slide seat is provided on the vertical slide seat, a bracket is fixed on the vertical slide seat, the bracket extends above the insulation groove, the bracket is L-shaped, and the clamp is fixed to the bottom end of the bracket.

[0010] Preferably, a protective shell is fixed on the bracket, a main motor is fixed on the top of the bracket, a vertical shaft is fixed to the output shaft of the main motor and extends into the protective shell, the vertical shaft is connected to the bracket through a main bearing seat, a main bevel gear is fixed to the bottom end of the vertical shaft, a horizontal shaft is rotatably connected to the bottom end of the bracket, one end of the horizontal shaft extends into the protective shell and a secondary bevel gear is fixed thereon, the secondary bevel gear meshes with the main bevel gear, and the other end of the horizontal shaft is fixedly connected to the outer wall of the ring.

[0011] Preferably, grippers are installed on both sides of the cover plate, and the cover plate is fixed to the ring by the grippers. The grippers include a vertical rod, a claw hook at the bottom of the vertical rod, and an inclined rod at the top of the vertical rod. The vertical rod is hinged to the edge of the cover plate, and a torsion spring is also installed between the inclined rod and the cover plate.

[0012] Preferably, the cover plate is provided with a suction assembly, which includes a pipette installed on the cover plate. The bottom end of the pipette extends into the sperm storage bottle. When the sperm is not completely liquefied, the suction assembly operates to help mix the sperm. When the sperm is completely liquefied, the suction assembly is also used to extract the liquefied sperm for placement on a glass slide.

[0013] Preferably, the straw passes through the cover plate and is rotatably connected to the cover plate. The bottom end of the straw is arc-shaped, and the top end of the straw extends above the cover plate and is fixed with a piston cylinder. A piston is provided inside the piston cylinder, and a spring is fixed between the piston and the inner top wall of the piston cylinder. An insertion rod is also fixed to the top of the piston. The top end of the insertion rod passes through the piston cylinder and is fixed with a top plate. The suction assembly also includes an auxiliary motor fixed to the cover plate. A crankshaft is fixed to the output shaft of the auxiliary motor. The crankshaft is mounted on the cover plate through a secondary bearing seat. A movable rod is provided on the crankshaft. The bottom end of the movable rod is movably connected to the crankshaft. A vertically arranged push rod is hinged to the top end of the movable rod. The top end of the push rod faces the bottom of the top plate. A sleeve is provided around the push rod. The sleeve is fixed to the cover plate by a fixing bracket. A first bevel gear is fixed to the end of the crankshaft, and a second bevel gear is fixed to the straw. The second bevel gear meshes with the first bevel gear.

[0014] Preferably, the fixing frame is provided with a vertical slide groove, and a strip slider is slidably connected to the vertical slide groove. The top end of the strip slider faces the bottom of the top plate, and a connecting rod is provided between the bottom end of the strip slider and the inclined rod. The two ends of the connecting rod are respectively hinged to the inclined rod and the strip slider.

[0015] Preferably, the piston cylinder sidewall is provided with a one-way port, the heat preservation box is also provided with a cleaning tank, a pump body is fixed inside the cleaning tank, an output pipe is fixed at the output end of the pump body, and the end of the output pipe is matched with the one-way port.

[0016] The present invention also discloses a method for detecting sperm motility, which includes sperm collection, liquefaction and microscopic observation, wherein the liquefaction step uses the aforementioned rapid sperm motility detection device.

[0017] Compared with the prior art, the present invention provides a rapid sperm motility detection device and its detection method, which has the following beneficial effects:

[0018] 1. This rapid sperm motility detection device and method, by setting up a drive component, a clamp, a cover plate, and a water immersion sensor installed on the cover plate, uses the clamp to fix the sperm storage bottle, and then uses the drive component to immerse the sperm storage bottle in a heat preservation tank to achieve liquefaction. The drive component can drive the sperm storage bottle to tilt intermittently and briefly inside the heat preservation tank. At this time, the water immersion sensor can detect the liquefied sperm, and the drive component can then move the sperm storage bottle out of the heat preservation tank again. This method is more accurate than manual observation of the liquefaction degree, and avoids frequent removal of the sperm storage bottle from the heat preservation tank, ensuring a stable liquefaction environment. Furthermore, it can remove the sperm immediately after liquefaction, avoiding excessively long or short liquefaction time, thus improving the accuracy of subsequent sperm motility detection.

[0019] 2. This rapid sperm motility detection device and method, by setting a suction component on the cover plate, allows for the removal of unhealthy sperm that leads to incomplete liquefaction when the collected sperm is activated. The suction component is then used to draw the sperm out through a pipette and reinject it into the bottom of the bottle, achieving a physical mixing effect. This mixing method is relatively gentle and avoids damage to sperm and uneven distribution of sperm due to centrifugation compared to manual shaking by the operator. This improves the accuracy of the test results and avoids misdiagnosis. The suction component also facilitates the extraction of liquefied sperm, making it easier to prepare slides for subsequent microscopic observation, thus making it more convenient to use.

[0020] 3. This rapid sperm motility detection device and its detection method, by setting up a one-way port, a cleaning tank, a pump body and an output pipe, when the pump body is running, pumps the cleaning solution in the cleaning tank to the output pipe, then pumps it into the piston cylinder, and finally flows out from the bottom of the pipette, which facilitates rinsing the pipette and avoids the interference of experimental results by residual sperm mixing with the sperm to be tested later. Attached Figure Description

[0021] Figure 1 This is a three-dimensional structural diagram of the sperm motility rapid detection device of the present invention;

[0022] Figure 2 This is a three-dimensional structural diagram of the insulated box of the present invention;

[0023] Figure 3 This is a cross-sectional view of the cover plate of the present invention;

[0024] Figure 4 For the present invention Figure 3 Enlarged view of part A;

[0025] Figure 5 This is a schematic diagram of the three-dimensional structure of the cover plate of the present invention;

[0026] Figure 6 This is a partial cross-sectional view of the driving component of the present invention;

[0027] Figure 7 This is a schematic diagram of the suction assembly of the present invention;

[0028] Figure 8 For the present invention Figure 7 Enlarged view of part B;

[0029] Figure 9 This is a schematic diagram of the operation of the driving component of the present invention;

[0030] Figure 10 This is a top view of the sperm motility rapid detection device of the present invention.

[0031] In the diagram: 1. Base; 2. Microscope; 3. Insulation box; 4. Insulation tank; 5. Clamp; 51. Ring; 52. Fastening bolt; 6. Drive assembly; 61. Vertical slide; 62. Vertical slide block; 63. Bracket; 64. Protective shell; 65. Main motor; 66. Vertical shaft; 67. Main bearing seat; 68. Main bevel gear; 69. Horizontal shaft; 610. Secondary bevel gear; 7. Cover plate; 70. Gripper; 701. Vertical rod; 702. Claw hook; 703. Diagonal rod; 704. Torsion spring; 8. Water immersion sensor 9. Suction assembly; 91. Suction tube; 92. Piston cylinder; 93. Piston; 94. Spring; 95. Insert rod; 96. Top plate; 97. Auxiliary motor; 98. Crankshaft; 99. Auxiliary bearing seat; 910. Movable rod; 911. Push rod; 912. Sleeve; 913. First bevel gear; 914. Second bevel gear; 915. Fixing frame; 916. Vertical slide; 917. Strip slider; 918. Connecting rod; 919. One-way nozzle; 10. Cleaning tank; 11. Pump body; 12. Output pipe. Detailed Implementation

[0032] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0033] As described in the background section, there are shortcomings in the existing technology. In order to solve the above-mentioned technical problems, this application proposes a sperm motility rapid detection device and its detection method.

[0034] Example 1: Please refer to Figures 1-5 A rapid sperm motility detection device includes a base 1, a microscope 2, and an incubator 3. The incubator 3 is provided with an incubation tank 4. A clamp 5, a drive assembly 6, and a cover plate 7 are provided above the incubator 3. The clamp 5 is used to hold a sperm storage bottle, and the cover plate 7 is used to seal the sperm storage bottle. A water immersion sensor 8 is fixedly installed on the inner edge of the cover plate 7. The drive assembly 6 is installed on one side of the incubator 3 and is used to drive the clamp 5 to move vertically and also to drive the clamp 5 to rotate.

[0035] Before sperm liquefaction, the sperm storage bottle is held by clamp 5 and the sperm storage bottle is sealed with cover plate 7;

[0036] During sperm liquefaction, the drive assembly 6 operates, forcing the clamp 5 and sperm storage bottle to move down into the heat preservation tank 4 for liquefaction. The operation of the drive assembly 6 also drives the clamp 5 to swing intermittently to an inclined state, forcing the sperm at the bottom of the sperm storage bottle to flow towards the cover plate 7. When the sperm flows to contact the water immersion sensor 8, the drive assembly 6 operates again, forcing the clamp 5 and sperm storage bottle to move out of the heat preservation tank 4.

[0037] The insulated tank 4 is filled with water. When the insulated box 3 is running, it forces the water temperature in the insulated tank 4 to be maintained at 37 degrees. The sperm storage bottle is existing technology. The drive component 6 is controlled by a PLC system. When the drive component 6 is running, the drive clamp 5 swings every 5 minutes, forcing the sperm storage bottle to tilt until the height of the bottle mouth is lower than the bottom of the bottle, and maintains this position for a period of time, such as 1 second. The cover plate 7 is equipped with a rubber ring that matches the sperm storage bottle.

[0038] In use, the sperm storage bottle is held by clamp 5, and then the cover plate 7 is used to seal the sperm storage bottle. At this time, the sperm storage bottle is in a vertical position. Then, the drive component 6 operates, driving clamp 5 to move vertically downward, moving the sperm storage bottle down into the heat preservation tank 4, and then the liquefaction process begins. After that, the drive component 6 operates every once in a while, driving clamp 5 and sperm storage bottle to swing, keeping the sperm storage bottle tilted and holding it for a short time. When the water immersion sensor 8 does not detect liquid, it indicates that the sperm liquefaction is insufficient, so the drive component 6 drives clamp 5 and sperm storage bottle to reset. If the water immersion sensor 8 detects liquid, it indicates that the sperm liquefaction is sufficient, so the drive component 6 operates, forcing clamp 5 and sperm storage bottle to reset and move upward to the outside of the heat preservation tank 4.

[0039] By setting up a drive assembly 6, a clamp 5, a cover plate 7, and a water immersion sensor 8 on the cover plate 7, the sperm storage bottle is fixed by the clamp 5, and then the drive assembly 6 is used to immerse the sperm storage bottle into the heat preservation tank 4 to achieve liquefaction. The drive assembly 6 can drive the sperm storage bottle to tilt intermittently and briefly inside the heat preservation tank 4. At this time, the water immersion sensor 8 can detect the liquefied sperm, and the drive assembly 6 can be used again to move the sperm storage bottle out of the heat preservation tank 4. This method is more accurate than manual observation of the liquefaction degree, and avoids frequently removing the sperm storage bottle from the heat preservation tank 4, ensuring the stability of the liquefaction environment. In addition, the sperm can be removed immediately after liquefaction, avoiding liquefaction time that is too long or too short, thus improving the accuracy of subsequent sperm motility testing.

[0040] Example 2: See Figure 6 Unlike the above embodiments, the clamp 5 includes a vertically arranged ring 51, and the side wall of the ring 51 is provided with a fastening bolt 52, which passes through the ring 51 and is threadedly connected to the ring 51.

[0041] The inner diameter of the ring 51 matches the diameter of the sperm storage bottle. In use, the sperm storage bottle is vertically inserted into the ring 51, and then the fastening bolt 52 is rotated to hold the sperm storage bottle, thereby fixing the sperm storage bottle. After fixing, the bottom of the sperm storage bottle faces down and the opening at the top faces up.

[0042] Example 3, see Figures 2-6 Unlike the above embodiments, the driving component 6 includes a vertical slide 61 fixed to the side wall of the insulation box 3, a vertical slide seat 62 is provided on the vertical slide 61, a bracket 63 is fixed on the vertical slide seat 62, the bracket 63 extends above the insulation groove 4, the bracket 63 is L-shaped, and the clamp 5 is fixed to the bottom end of the bracket 63.

[0043] The vertical slide 61 is an electric slide. When in use, the electric slide drives the vertical slide 62 to slide downwards. The vertical slide 62 drives the support 63 to move downwards. The support 63 drives the clamp 5 and the sperm storage bottle to move downwards, so that the sperm storage bottle is immersed in the heat preservation tank 4 to realize the liquefaction process.

[0044] Example 4, see Figure 6 and Figure 9 Unlike the above embodiments, a protective shell 64 is fixed on the bracket 63, a main motor 65 is fixed on the top of the bracket 63, a vertical shaft 66 is fixed to the output shaft of the main motor 65 and extends into the protective shell 64, the vertical shaft 66 is connected to the bracket 63 through a main bearing seat 67, a main bevel gear 68 is fixed to the bottom end of the vertical shaft 66, a horizontal shaft 69 is rotatably connected to the bottom end of the bracket 63, one end of the horizontal shaft 69 extends into the protective shell 64 and a secondary bevel gear 610 is fixed thereon, the secondary bevel gear 610 meshes with the main bevel gear 68, and the other end of the horizontal shaft 69 is fixedly connected to the outer wall of the ring 51.

[0045] The protective shell 64 is sealed at the connection with the bracket 63, thus providing protection for the vertical shaft 66 and the horizontal shaft 69. When it is necessary to detect the degree of sperm liquefaction, the main motor 65 is started. When the main motor 65 is running, it drives the vertical shaft 66 and the main bevel gear 68 to rotate. When the main bevel gear 68 rotates, it drives the secondary bevel gear 610 to rotate. When the secondary bevel gear 610 rotates, it drives the horizontal shaft 69 to rotate. When the horizontal shaft 69 rotates, it drives the clamp 5 to rotate, causing the sperm storage bottle to flip to an incline. At this time, the height of the cover plate 7 of the sperm storage bottle is lower than the bottom of the bottle, so the sperm at the bottom of the bottle flows towards the cover plate 7. If liquefaction is incomplete, the sperm has poor fluidity and cannot contact the water immersion sensor 8 on the cover plate 7 within a unit time. Then the main motor 65 runs again, controlling the sperm storage bottle to reset and continue liquefaction. If liquefaction is complete, the main motor 65 controls the sperm storage bottle to reset, and at the same time, the vertical slide 61 controls the sperm storage bottle to move upward to the outside of the heat preservation tank 4.

[0046] By setting the main motor 65, the sperm storage bottle can be driven to rotate and tilt inside the heat preservation tank 4, which, together with the water immersion sensor 8, can detect the fluidity of the sperm, which is helpful in judging the degree of sperm liquefaction. After the sperm is completely liquefied, the sperm can be driven to leave the heat preservation tank 4 in time, which has a high degree of automation.

[0047] Example 5, see Figures 5-7 Unlike the above embodiments, the cover plate 7 is equipped with claws 70 on both sides. The cover plate 7 is fixed to the ring 51 by the claws 70. The claws 70 include a vertical rod 701, a claw hook 702 set at the bottom of the vertical rod 701, and an inclined rod 703 set at the top of the vertical rod 701. The vertical rod 701 is hinged to the edge of the cover plate 7. A torsion spring 704 is also installed between the inclined rod 703 and the cover plate 7.

[0048] When in use, manually press the inclined rod 703 part on the two grippers 70 to make the grippers 70 swing, then place the cover plate 7 on the sperm storage bottle, release the grippers 70, the elastic force of the torsion spring 704 makes the grippers 70 return to their original position, the hook on the grippers 70 clamps the edge of the ring 51 on the clamp 5, and fixes the cover plate 7 on the sperm storage bottle, which is conducive to the installation of the cover plate 7.

[0049] Example 6, see Figures 2-8 Unlike the above embodiments, the cover plate 7 is provided with a suction assembly 9. The suction assembly 9 includes a pipette 91 installed on the cover plate 7. The bottom end of the pipette 91 extends into the sperm storage bottle. When the sperm liquefaction is incomplete, the suction assembly 9 operates to assist in mixing the sperm. When the sperm liquefaction is complete, the suction assembly 9 is also used to extract the liquefied sperm for placement on a glass slide. The pipette 91 passes through the cover plate 7 and is rotatably connected to the cover plate 7. The bottom end of the pipette 91 is arc-shaped. The top end of the pipette 91 extends above the cover plate 7 and is fixed with a piston cylinder 92. A piston 93 is provided inside the piston cylinder 92. A spring 94 is fixed between the piston 93 and the inner top wall of the piston cylinder 92. An insertion rod 95 is also fixed to the top of the piston 93. The top end of the insertion rod 95 passes through the piston cylinder 92. The suction assembly 9 also includes a top plate 96 fixedly attached to the cover plate 7. An auxiliary motor 97 is fixed to the output shaft of the auxiliary motor 97, which is connected to a crankshaft 98. The crankshaft 98 is mounted on the cover plate 7 via a secondary bearing seat 99. A movable rod 910 is mounted on the crankshaft 98, with its bottom end movably connected to the crankshaft 98. A vertically mounted push rod 911 is hinged to the top of the movable rod 910, with its top end facing the bottom of the top plate 96. A sleeve 912 is fitted over the push rod 911, and the sleeve 912 is fixed to the cover plate 7 via a fixing bracket 915. A first bevel gear 913 is fixed to the end of the crankshaft 98, and a second bevel gear 914 is fixed to the suction tube 91, meshing with the first bevel gear 913.

[0050] When the cover plate 7 is installed, the straw 91 is inserted into the sperm storage bottle. If the sperm does not liquefy completely within the normal time, physical means are needed to assist liquefaction in order to ensure the subsequent observation of sperm activity. At this time, the auxiliary motor 97 is started. When the auxiliary motor 97 is running, it drives the crankshaft 98 to rotate. When the crankshaft 98 rotates, it drives the bottom end of the movable rod 910 to move back and forth. The top end of the movable rod 910 pushes the push rod 911 to move vertically back and forth. When the push rod 911 moves upward, it pushes the top plate 96. The top plate 96 drives the insertion rod 95 and the piston 93 to move upward, generating suction and drawing the sperm into the straw. In step 91, the piston 93 moves upward and compresses the spring 94. When the push rod 911 moves downward, the elastic force of the spring 94 pushes the piston 93 back to its original position, generating a thrust to re-inject the sperm into the bottom of the bottle. At the same time, when the crankshaft 98 rotates, it also drives the first bevel gear 913 to rotate. When the first bevel gear 913 rotates, it drives the second bevel gear 914 to rotate. When the second bevel gear 914 rotates, it drives the straw 91 and the piston cylinder 92 to rotate. When the straw 91 rotates, the position of its bottom end changes continuously. Therefore, the sperm drawn into the straw 91 is re-injected into different positions at the bottom of the bottle, which has a mixing effect.

[0051] By setting the suction component 9 on the cover plate 7, if the collected sperm is unhealthy and liquefaction is incomplete, the suction component 9 can be activated to draw out the sperm through the pipette 91 and reinject it into the bottom of the bottle, achieving a physical mixing effect. This mixing method is relatively gentle and can avoid damaging the sperm and uneven distribution of sperm due to centrifugation compared to the manual shaking method used by the operator, thus improving the accuracy of the test results and avoiding misdiagnosis.

[0052] Example 7, see Figure 7 Unlike the above embodiments, the fixing frame 915 is provided with a vertical slide groove 916, and a strip slider 917 is slidably connected to the vertical slide groove 916. The top end of the strip slider 917 faces the bottom of the top plate 96, and a connecting rod 918 is provided between the bottom end of the strip slider 917 and the inclined rod 703. The two ends of the connecting rod 918 are respectively hinged to the inclined rod 703 and the strip slider 917.

[0053] After liquefaction is complete, press the inclined rod 703 on the two grippers 70 to tilt the rod 703. At this time, the grippers 70 disengage from the clamp 5. Continue pressing, and when the inclined rod 703 tilts, it pushes the connecting rod 918. The other end of the connecting rod 918 pushes the strip slider 917. After the strip slider 917 moves upward, it pushes the top plate 96 upward. Similarly, when the top plate 96 moves upward, the bottom end of the pipette 91 generates suction, which draws out the sperm. At this time, the operator moves the cover plate 7 onto the glass slide, releases the grippers 70, and the top plate 96 returns to its original position. The sperm overflowing from the bottom end of the pipette 91 drips onto the glass slide, which is convenient for subsequent observation on the microscope 2. The operation is convenient and improves the efficiency of the experiment.

[0054] Example 8, see Figure 10 Unlike the above embodiments, the piston cylinder 92 has a one-way port 919 on its side wall, and the insulation box 3 is also provided with a cleaning tank 10. A pump body 11 is fixed inside the cleaning tank 10, and an output pipe 12 is fixed at the output end of the pump body 11. The end of the output pipe 12 matches the one-way port 919.

[0055] In the initial state, there is a cavity between the piston 93 and the bottom of the piston cylinder 92. The one-way port 919 corresponds to the position of the cavity. The one-way port 919 only allows the medium to enter the piston cylinder 92 under pressure. In use, after the entire liquefaction operation is completed, the output pipe 12 is inserted into the one-way port 919 to achieve connection. Then, the pump body 11 is started. When the pump body 11 is running, the cleaning liquid in the cleaning tank 10 is pumped to the output pipe 12 and then pumped into the piston cylinder 92. Finally, it flows out from the bottom of the pipette 91, which is convenient for rinsing the pipette 91 and avoids the residual sperm from mixing with the sperm to be tested later and interfering with the experimental results.

[0056] Example 9: A method for detecting sperm motility, comprising sperm collection, liquefaction, and microscopic observation, wherein the liquefaction step uses a rapid sperm motility detection device from the above examples.

[0057] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A rapid sperm motility detection device, comprising a base, a microscope, and an incubator, characterized in that: The insulated box is provided with an insulated groove. A clamp, a drive assembly, and a cover plate are provided on the top of the insulated box. The clamp is used to hold the sperm storage bottle, and the cover plate is used to seal the sperm storage bottle. A water immersion sensor is fixedly installed on the inner edge of the cover plate. The drive assembly is installed on one side of the insulated box. The drive assembly is used to drive the clamp to move vertically and also to drive the clamp to rotate. Before sperm liquefaction, the sperm storage bottle is held in place by clamps and the cap is sealed on the sperm storage bottle; During sperm liquefaction, the drive assembly operates, forcing the clamp and sperm storage bottle to move down into the insulated tank for liquefaction. The operation of the drive assembly also causes the clamp to swing intermittently to an inclined state, forcing the sperm at the bottom of the sperm storage bottle to flow towards the cover plate. When the sperm flows to contact the water immersion sensor, the drive assembly operates again, forcing the clamp and sperm storage bottle to move out of the insulated tank.

2. The sperm motility rapid detection device according to claim 1, characterized in that: The clamp includes a vertically arranged ring, and a fastening bolt is provided on the side wall of the ring. The fastening bolt passes through the ring and is threadedly connected to the ring.

3. The sperm motility rapid detection device according to claim 2, characterized in that: The drive assembly includes a vertical slide fixed to the side wall of the insulation box, a vertical slide base is provided on the vertical slide base, a bracket is fixed on the vertical slide base, the bracket extends above the insulation groove, the bracket is L-shaped, and the clamp is fixed to the bottom end of the bracket.

4. The sperm motility rapid detection device according to claim 3, characterized in that: A protective shell is fixed on the bracket, and a main motor is fixed on the top of the bracket. The output shaft of the main motor is fixed with a vertical shaft and extends into the protective shell. The vertical shaft is connected to the bracket through a main bearing seat. A main bevel gear is fixed at the bottom of the vertical shaft. A horizontal shaft is rotatably connected to the bottom of the bracket. One end of the horizontal shaft extends into the protective shell and is fixed with a secondary bevel gear. The secondary bevel gear meshes with the main bevel gear. The other end of the horizontal shaft is fixedly connected to the outer wall of the ring.

5. The sperm motility rapid detection device according to claim 4, characterized in that: The cover plate is equipped with grippers on both sides, and the cover plate is fixed to the ring by the grippers. The grippers include a vertical rod, a claw hook at the bottom of the vertical rod, and an inclined rod at the top of the vertical rod. The vertical rod is hinged to the edge of the cover plate, and a torsion spring is also installed between the inclined rod and the cover plate.

6. The sperm motility rapid detection device according to claim 5, characterized in that: The cover plate is provided with a suction assembly, which includes a pipette installed on the cover plate. The bottom end of the pipette extends into the sperm storage bottle. When the sperm is not completely liquefied, the suction assembly operates to help mix the sperm. When the sperm is completely liquefied, the suction assembly is also used to extract the liquefied sperm for the preparation of a glass slide.

7. The sperm motility rapid detection device according to claim 6, characterized in that: The straw passes through the cover plate and is rotatably connected to it. The bottom end of the straw is arc-shaped, and the top end of the straw extends above the cover plate and is fixed to a piston cylinder. A piston is provided inside the piston cylinder, and a spring is fixed between the piston and the inner top wall of the piston cylinder. An insert rod is also fixed to the top of the piston, and the top end of the insert rod passes through the piston cylinder and is fixed to a top plate. The suction assembly also includes an auxiliary motor fixed to the cover plate. A crankshaft is fixed to the output shaft of the auxiliary motor. The crankshaft is mounted on the cover plate through a secondary bearing seat. A movable rod is provided on the crankshaft. The bottom end of the movable rod is movably connected to the crankshaft. A vertically arranged push rod is hinged to the top end of the movable rod. The top end of the push rod faces the bottom of the top plate. A sleeve is provided around the push rod. The sleeve is fixed to the cover plate by a fixing bracket. A first bevel gear is fixed to the end of the crankshaft, and a second bevel gear is fixed to the straw. The second bevel gear meshes with the first bevel gear.

8. The sperm motility rapid detection device according to claim 7, characterized in that: The fixed frame is provided with a vertical slide groove, and a strip slider is slidably connected to the vertical slide groove. The top of the strip slider faces the bottom of the top plate, and a connecting rod is provided between the bottom of the strip slider and the inclined rod. The two ends of the connecting rod are respectively hinged to the inclined rod and the strip slider.

9. The sperm motility rapid detection device according to claim 7, characterized in that: The piston cylinder has a one-way port on its side wall, and the insulation box is also equipped with a cleaning tank. A pump body is fixed inside the cleaning tank, and an output pipe is fixed at the output end of the pump body. The end of the output pipe matches the one-way port.

10. A method for detecting sperm motility, characterized in that: This sperm motility detection method includes sperm collection, liquefaction, and microscopic observation, wherein the liquefaction step uses a sperm motility rapid detection device as described in any one of claims 1-9.

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