Angelica pandshurica leaf aromatic product with anti-depression effect and preparation method thereof

Abstract

The invention discloses a pandan leaf aromatic product with an anti-depression effect and a preparation method of the pandan leaf aromatic product. The pandan leaves are extracted through supercritical carbon dioxide fluid under the conditions of specific temperature and pressure, anti-depression aroma components of the pandan leaves are effectively extracted, the pandan leaf extract rich in aroma and having the anti-depression effect is obtained, and the pandan leaf extract can be widely applied to the fields of cosmetics, medicine and the like after being compounded with citrus essential oil and cananga odorata essential oil. The product is natural and safe, and provides a new choice for emotion regulation and adjuvant therapy of depression.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of medicine and cosmetics, and particularly relates to a kind of pandanus amaryllifolius aromatic product with anti-depression effect and a preparation method thereof. BACKGROUND

[0002] Depression, as a common mental disorder, seriously affects the physical and mental health and quality of life of patients. In recent years, aromatherapy as an auxiliary treatment for depression has received more and more attention. Studies have shown that aromatic substances can act on the brain limbic system through the olfactory system, and then affect mood and psychological state. The aroma of some plants can positively affect people's mood, for example, lavender essential oil is reported to have a certain soothing effect and can reduce anxiety. However, current aromatic products mostly focus on improving indoor odor, body odor or simply refreshing the mind, and lack research on anti-depression. Even some products claim to have a certain soothing effect, but the therapeutic effect is limited.

[0003] Pandanus amaryllifolius, as a plant with unique aroma and rich nutritional value, is widely used in food seasoning, traditional medicine and natural perfume making in Southeast Asia. With the increasing demand for natural and healthy products, the development and utilization value of pandanus amaryllifolius is increasingly prominent. However, there is currently a lack of research on the anti-depression effect of pandanus amaryllifolius aromatic products.

[0004] Therefore, it is of great practical significance to develop a pandanus amaryllifolius aromatic product with clear anti-depression effect, effective retention of active ingredients and rich fragrance, and a preparation method thereof. SUMMARY

[0005] In view of the shortcomings of the prior art, the present application provides a pandanus amaryllifolius aromatic product with anti-depression effect and a preparation method thereof.

[0006] The technical scheme of the present application is as follows:

[0007] A pandanus amaryllifolius aromatic product with anti-depression effect contains pandanus amaryllifolius essential oil, citrus essential oil and ylang ylang essential oil, and the pandanus amaryllifolius essential oil is obtained by supercritical carbon dioxide fluid extraction.

[0008] Preferably, the volume ratio of pandanus amaryllifolius essential oil, citrus essential oil and ylang ylang essential oil is 10:1 to 2:0.5 to 1.

[0009] Preferably, the pandanus amaryllifolius aromatic product further contains water; and the volume ratio of pandanus amaryllifolius essential oil, citrus essential oil, ylang ylang essential oil and water is 10:1 to 2:0.5 to 1:80 to 100.

[0010] Preferably, the extraction method of pandanus amaryllifolius essential oil comprises the following steps:

[0011] (1) Take fresh leaves of the plantain, wash and dry, then cut into small pieces to increase the contact area with supercritical fluid and improve the extraction efficiency;

[0012] (2) Add an ethanol aqueous solution to the small pieces of the plantain leaves, mix well to obtain a mixture; the ethanol aqueous solution contains acetic acid;

[0013] (3) Put the mixture into an extraction kettle, and pass a supercritical carbon dioxide fluid to extract, the extraction pressure is 25-35 MPa, the extraction temperature is 35-55℃, and the extraction time is 2-3 hours;

[0014] (4) The mixed fluid after extraction is first introduced into a separation kettle I, in which the separation pressure is 13-15 MPa and the separation temperature is 40-50℃, so that the extract with reduced solubility is separated from the supercritical carbon dioxide fluid. Then, the fluid is introduced into a separation kettle II, in which the separation pressure is 8-9 MPa and the separation temperature is 30-40℃, so that the remaining extract is separated and precipitated from the fluid, and the precipitated product is the target extract.

[0015] Preferably, the ethanol aqueous solution contains 5-10 vol% acetic acid.

[0016] Preferably, the flow rate of the supercritical carbon dioxide fluid is 10-30 L / h, so that the fluid can fully contact with the plant raw material and improve the mass transfer efficiency.

[0017] Preferably, in step (2), the ethanol aqueous solution accounts for 10-15% of the mass of the plantain leaves. The ethanol aqueous solution is an ethanol aqueous solution with a volume fraction of 70-90%.

[0018] Preferably, the extraction method of the citrus essential oil and the ylang essential oil comprises the following steps:

[0019] (1) Take fresh citrus peels or ylang whole plants, wash and dry, then cut into small pieces;

[0020] (2) Add an ethanol aqueous solution to the small pieces, mix well to obtain a mixture; the ethanol aqueous solution contains acetic acid;

[0021] (3) Put the mixture into an extraction kettle, and pass a supercritical carbon dioxide fluid to extract, the extraction pressure is 25-35 MPa, the extraction temperature is 35-55℃, and the extraction time is 2-3 hours;

[0022] (4) The mixed fluid after extraction is first introduced into a separation kettle I, in which the separation pressure is 10-15 MPa and the separation temperature is 40-50℃, then introduced into a separation kettle II, in which the separation pressure is 8-9 MPa and the separation temperature is 30-40℃, to obtain the citrus essential oil or the ylang essential oil.

[0023] The preparation method of the patchouli leaf fragrance product comprises the following steps: mixing the components, and stirring uniformly to obtain the patchouli leaf fragrance product.

[0024] In another aspect, the present application relates to the use of a composition containing patchouli leaf essential oil in the preparation of an anti-depression product.

[0025] Compared with the prior art, the present application has the beneficial results that:

[0026] The present application selectively extracts the aromatic active components of patchouli leaf, citrus and ylang-ylang by using supercritical carbon dioxide fluid extraction method, so as to ensure the pure fragrance and anti-depression effect of the product. Animal experiments show that after the continuous use of the product for 14 days, the immobile time of the experimental animals in the forced swimming test and tail suspension test is significantly shortened, which indicates that the depression of the experimental animals is obviously relieved.

[0027] The product of the present application has strong fragrance memory and rich fragrance levels, and can be used as daily perfume. DETAILED DESCRIPTION

[0028] In order to better understand the technical content of the present application, the present application will be further described below in combination with specific examples.

[0029] Example 1: Preparation of patchouli leaf fragrance product

[0030] (1) Fresh patchouli leaves are washed, dried and cut into small pieces with a length of 0.5-2 cm.

[0031] (2) 15% (by mass) of 70-90% (by volume) ethanol aqueous solution is added to the patchouli leaf pieces, and the mixture is obtained after uniform mixing. The ethanol aqueous solution contains 10% (by volume) acetic acid.

[0032] (3) The mixture is placed into an extraction kettle, and supercritical carbon dioxide fluid is introduced for extraction. The extraction pressure is 25 MPa, the extraction temperature is 55°C, the extraction time is 3 hours, and the flow rate of the supercritical carbon dioxide fluid is 20 L / h.

[0033] (4) The mixed fluid after extraction is first introduced into separation kettle I with a separation pressure of 15 MPa and a separation temperature of 50°C, and then introduced into separation kettle II with a separation pressure of 8 MPa and a separation temperature of 40°C. Finally, patchouli leaf essential oil is obtained.

[0034] (5) 10 ml of patchouli leaf essential oil is added to 80 ml of deionized water, and the mixture is stirred uniformly to obtain the patchouli leaf fragrance product.

[0035] Example 2: Preparation of patchouli leaf fragrance product

[0036] (1) Fresh patchouli leaves are washed, dried and cut into small pieces with a length of 0.5-2 cm.

[0037] (2) Add 70-90% ethanol aqueous solution with 10% acetic acid to the small pieces of leaves of Pogostemonis Herba, and mix well to obtain a mixture.

[0038] (3) Put the mixture into an extraction kettle, and extract with supercritical carbon dioxide fluid. The extraction pressure is 25 MPa, the extraction temperature is 55°C, the extraction time is 3 hours, and the flow rate of the supercritical carbon dioxide fluid is 20 L / h.

[0039] (4) The mixed fluid after extraction is first introduced into separation kettle I with a separation pressure of 15 MPa and a separation temperature of 50°C, and then introduced into separation kettle II with a separation pressure of 8 MPa and a separation temperature of 40°C. Finally, the essential oil of Pogostemonis Herba is obtained.

[0040] The extraction steps of the citrus essential oil (raw material is the pericarp of Citrus bergamia) and the ylang-ylang essential oil (raw material is the whole plant of Cananga odorata) are the same as those of the essential oil of Pogostemonis Herba.

[0041] (5) Add 1 ml of the citrus essential oil and 0.5 ml of the ylang-ylang essential oil to 10 ml of the essential oil of Pogostemonis Herba. Then, add the mixture to 80 ml of deionized water, and stir well to obtain the Pogostemonis Herba fragrance product.

[0042] Example 3 Preparation of Pogostemonis Herba fragrance product

[0043] (1) Take fresh Pogostemonis Herba, wash and dry, and cut into small pieces with a length of 0.5-2 cm.

[0044] (2) Add 70-90% ethanol aqueous solution with 10% acetic acid to the small pieces of leaves of Pogostemonis Herba, and mix well to obtain a mixture.

[0045] (3) Put the mixture into an extraction kettle, and extract with supercritical carbon dioxide fluid. The extraction pressure is 25 MPa, the extraction temperature is 55°C, the extraction time is 3 hours, and the flow rate of the supercritical carbon dioxide fluid is 20 L / h.

[0046] (4) The mixed fluid after extraction is first introduced into separation kettle I with a separation pressure of 15 MPa and a separation temperature of 50°C, and then introduced into separation kettle II with a separation pressure of 8 MPa and a separation temperature of 40°C. Finally, the essential oil of Pogostemonis Herba is obtained.

[0047] The extraction steps of the citrus essential oil (raw material is the pericarp of Citrus bergamia) and the ylang-ylang essential oil (raw material is the whole plant of Cananga odorata) are the same as those of the essential oil of Pogostemonis Herba.

[0048] (5) Take 10 ml of patchouli leaf extract, add 1 ml of citrus oil and 0.5 ml of ylang-ylang oil. Then add the mixture to 80 ml of deionized water, and stir well to obtain a patchouli leaf fragrance product.

[0049] Example 4 Preparation of patchouli leaf fragrance product

[0050] (1) Take fresh patchouli leaves, wash and dry, and cut into small pieces of 0.5-2 cm in length.

[0051] (2) Add 70-90% ethanol aqueous solution with a volume fraction of 15% of the mass of the patchouli leaves to the small pieces of patchouli leaves, and mix well to obtain a mixture. The ethanol aqueous solution contains 10% acetic acid.

[0052] (3) Put the mixture into an extraction kettle, and pass supercritical carbon dioxide fluid to extract, with an extraction pressure of 25 MPa, an extraction temperature of 55°C, an extraction time of 3 hours, and a supercritical carbon dioxide fluid flow rate of 20 L / h.

[0053] (4) The extracted mixture first enters separation kettle I, with a separation pressure of 15 MPa and a separation temperature of 50°C, and then enters separation kettle II, with a separation pressure of 8 MPa and a separation temperature of 40°C. Finally, patchouli leaf essential oil is obtained.

[0054] The extraction steps of citrus oil (raw material: bergamot peel) and ylang-ylang oil (raw material: ylang-ylang whole plant) are similar to those of patchouli leaf essential oil, except that the separation pressure of separation kettle I is 10 MPa and the separation temperature is 40°C, and then separation kettle II is entered, with a separation pressure of 8 MPa and a separation temperature of 30°C.

[0055] (5) Take 10 ml of patchouli leaf extract, add 1 ml of citrus oil and 0.5 ml of ylang-ylang oil. Then add the mixture to 80 ml of deionized water, and stir well to obtain a patchouli leaf fragrance product.

[0056] Example 5 Preparation of patchouli leaf fragrance product

[0057] (1) Take fresh patchouli leaves, wash and dry, and cut into small pieces of 0.5-2 cm in length.

[0058] (2) Add 70-90% ethanol aqueous solution with a volume fraction of 15% of the mass of the patchouli leaves to the small pieces of patchouli leaves, and mix well to obtain a mixture. The ethanol aqueous solution contains 10% acetic acid.

[0059] (3) Put the mixture into an extraction kettle, and pass supercritical carbon dioxide fluid to extract, with an extraction pressure of 35 MPa, an extraction temperature of 35°C, an extraction time of 3 hours, and a supercritical carbon dioxide fluid flow rate of 20 L / h.

[0060] (4) The mixed fluid after extraction is first introduced into separator I at a separation pressure of 13 MPa and a separation temperature of 40°C, and then introduced into separator II at a separation pressure of 9 MPa and a separation temperature of 30°C. Finally, the patchouli leaf essential oil is obtained.

[0061] The extraction steps of the citrus essential oil (raw material: bergamot peel) and the ylang-ylang essential oil (raw material: ylang-ylang whole plant) are the same as those of the patchouli leaf essential oil.

[0062] (5) 10 ml of the patchouli leaf essential oil is taken, 1 ml of the citrus essential oil and 0.5 ml of the ylang-ylang essential oil are added. The mixture is then added to 80 ml of deionized water, and stirred uniformly to obtain the patchouli leaf fragrance product.

[0063] Example 6: Preparation of patchouli leaf fragrance product

[0064] (1) Fresh patchouli leaves are washed, dried and cut into small pieces of 0.5-2 cm in length.

[0065] (2) 10% by volume of 70-90% ethanol aqueous solution based on the mass of the patchouli leaves is added to the patchouli leaf pieces, and the mixture is stirred uniformly to obtain a mixture. The ethanol aqueous solution contains 5% by volume of acetic acid.

[0066] (3) The mixture is introduced into an extraction kettle, and supercritical carbon dioxide fluid is introduced for extraction at an extraction pressure of 25 MPa, an extraction temperature of 55°C, an extraction time of 1.5 hours and a supercritical carbon dioxide fluid flow rate of 20 L / h.

[0067] (4) The mixed fluid after extraction is first introduced into separator I at a separation pressure of 15 MPa and a separation temperature of 50°C, and then introduced into separator II at a separation pressure of 8 MPa and a separation temperature of 40°C. Finally, the patchouli leaf essential oil is obtained.

[0068] The extraction steps of the citrus essential oil (raw material: bergamot peel) and the ylang-ylang essential oil (raw material: ylang-ylang whole plant) are the same as those of the patchouli leaf essential oil.

[0069] (5) 10 ml of the patchouli leaf essential oil is taken, 1 ml of the citrus essential oil and 0.5 ml of the ylang-ylang essential oil are added. The mixture is then added to 100 ml of deionized water, and stirred uniformly to obtain the patchouli leaf fragrance product.

[0070] Example 7:

[0071] In this example, the patchouli leaf essential oil is extracted by steam distillation. The extraction conditions are as follows: 5 times the weight of water is added, and distillation is carried out at 120°C for 5 hours. The extraction steps of the citrus essential oil (raw material: bergamot peel) and the ylang-ylang essential oil (raw material: ylang-ylang whole plant) are the same as those of the patchouli leaf essential oil.

[0072] Take 10 ml of patchouli leaf essential oil, add 1 ml of citrus essential oil and 0.5 ml of ylang essential oil. Then add this mixture to 80 ml of deionized water, stir evenly, and you get a patchouli leaf fragrance product.

[0073] Example 8: Preparation of patchouli leaf fragrance product

[0074] (1) Take fresh patchouli leaves, wash and dry, then cut into small pieces of 0.5-2 cm in length.

[0075] (2) Add 70-90% ethanol aqueous solution with a volume fraction of 15% of the mass of patchouli leaves to the patchouli leaf pieces, mix evenly, and obtain a mixture.

[0076] (3) Put the mixture into an extraction kettle and pass supercritical carbon dioxide fluid for extraction, with an extraction pressure of 25 MPa, an extraction temperature of 55°C, an extraction time of 3 hours, and a supercritical carbon dioxide fluid flow rate of 20 L / h.

[0077] (4) After extraction, the mixed fluid first enters separation kettle I at a separation pressure of 15 MPa and a separation temperature of 50°C, and then enters separation kettle II at a separation pressure of 8 MPa and a separation temperature of 40°C. Finally, patchouli leaf essential oil is obtained.

[0078] The extraction steps of citrus essential oil (raw material is bergamot peel) and ylang essential oil (raw material is ylang whole plant) are the same as those of patchouli leaf essential oil.

[0079] (5) Take 10 ml of patchouli leaf essential oil, add 1 ml of citrus essential oil and 0.5 ml of ylang essential oil. Then add this mixture to 80 ml of deionized water, stir evenly, and you get a patchouli leaf fragrance product.

[0080] Experiment 1: Animal experiment

[0081] Test method:

[0082] Select KM male mice, SPF level, body weight 20±1.1 g, single cage feeding, adapt to feeding for 1 week, and select mice with close indicators through forced swimming test and tail suspension test for experiment. Randomly select 5 mice as normal group. Other mice are established into depression model by chronic unpredictable mild stress model (CUMS) + solitary feeding (single mouse single cage feeding). Mice receive a random stimulus every day, and the stimulus does not repeat for two adjacent days. The stimulus includes: fasting and water deprivation for 24 h, cold water swimming (4°C, 5 min), hot water swimming (45°C, 5 min), day-night rhythm reversal, shaking (5 min), tail clamping (3 min), and continuous stimulation for 28 days. The success criteria of the model are: after 28 days of stimulation, compared with the mice before modeling, the immobile time of forced swimming test and tail suspension test is significantly prolonged, and the body weight is significantly reduced.

[0083] The mice with successful modeling were randomly divided into a model group and a treatment group, 5 mice in each group. The mice in the model group were not given treatment and were allowed to drink water and eat normally. The mice in the treatment group were given atomization treatment with the fragrance product. 500 μL of the fragrance product was atomized with an atomizer to release it into the environment of the cage, and the mice were given the product once every 8 hours, for 14 consecutive days. The mice were allowed to drink water and eat normally during the treatment.

[0084] Forced swimming test (FST):

[0085] The mice were placed in a transparent round bucket with a diameter of 10 cm x height of 25 cm, and the bucket was filled with water to a depth of 15 cm. The water temperature was 22-25°C. The activity of the mice was recorded for 6 minutes. If the mouse floated on the water surface without moving or without obvious struggling (only slight limb movement), it was considered to be in a stationary state. The cumulative immobility time of the mice in the last 4 minutes was counted.

[0086] Tail suspension test (TST):

[0087] The mice were fixed at a distance of 1.5 cm from the tail tip with adhesive tape, and the mice were hung upside down on a hook with their heads pointing downward, 30 cm from the ground. The activity of the mice was recorded for 6 minutes. If the mouse did not struggle significantly when it was hung upside down, it was considered to be in a stationary state. The tail suspension immobility time was counted for the last 4 minutes.

[0088] The body weight of the mice in each group was recorded before and after the experiment. All results were expressed as mean ± standard deviation, and the significance of the differences between the groups was compared using analysis of variance.

[0089] Experimental results:

[0090] After 14 days of treatment, the body weight of the mice in the treatment group was significantly increased compared with the model group (P<0.05), and tended to be normal. The forced swimming immobility time of the mice was significantly reduced (P<0.05), and the tail suspension immobility time of the mice was significantly reduced (P<0.05).

[0091] Among the treatment groups, the treatment effects of Examples 2-5 were good, the body weight was significantly higher than that of the model group and close to that of the normal group, the forced swimming immobility time was significantly lower than that of Examples 7 and 8 (P<0.01), and the tail suspension immobility time was significantly lower than that of Examples 7 and 8 (P<0.01). By comparing Example 1, Example 2, and Example 8, it was found that the use of an aqueous solution of ethanol containing acetic acid for treatment and the supercritical fluid extraction of the extract of the leaves of the plant Pothos chinensis resulted in a synergistic effect when combined with citrus essential oil and ylang ylang essential oil.

[0092] Among Examples 2-5, Example 4 had the best antidepressant effect. The forced swimming immobility time and the tail suspension immobility time were both significantly lower than those of the other treatment groups.

[0093] Table 1 Comparison of depression indicators of mice in each group after the experiment

[0094]

[0095]

[0096] * represents significant difference (P<0.05) compared with the model group

[0097] The above merely describes some embodiments of the present application and is not intended to limit the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall fall within the protection scope of the present application.

Claims

1. A pandan leaf aromatic product with antidepressant effects, characterized in that, It contains pandan leaf essential oil, citrus essential oil and ylang-ylang essential oil, wherein the pandan leaf essential oil is obtained by supercritical carbon dioxide fluid extraction.

2. The pandanus leaf aromatic product according to claim 1, characterized in that, The volume ratio of pandan leaf essential oil, citrus essential oil, and ylang-ylang essential oil is 10:1~2:0.5~1.

3. The pandanus leaf aromatic product according to claim 1, characterized in that, The pandan leaf aromatic product also contains water; the volume ratio of pandan leaf essential oil, citrus essential oil, ylang-ylang essential oil, and water is 10:1~2:0.5~1:80~100.

4. The pandanus leaf aromatic product according to claim 1, characterized in that, The extraction method for pandan leaf essential oil includes the following steps: (1) Take fresh pandan leaves, wash and dry them, then cut them into small pieces; (2) Add an ethanol-water solution to the small pieces of Panax notoginseng leaves, mix well, and obtain a mixture; the ethanol-water solution contains acetic acid; (3) Place the mixture into the extraction vessel, introduce supercritical carbon dioxide fluid for extraction, the extraction pressure is 25-35 MPa, the extraction temperature is 35-55℃, and the extraction time is 2-3 hours; (4) The extracted mixed fluid first enters separation vessel I, with a separation pressure of 13-15 MPa and a separation temperature of 40-50℃, and then enters separation vessel II, with a separation pressure of 8-9 MPa and a separation temperature of 30-40℃, to obtain pandan leaf essential oil.

5. The pandanus leaf aromatic product according to claim 4, characterized in that, The ethanol-water solution contains 5-10 wt% acetic acid; in step (2), the ethanol-water solution accounts for 10%-15% of the mass of the pandanus leaves.

6. The pandanus leaf aromatic product according to claim 1, characterized in that, The extraction methods for citrus essential oil and ylang-ylang essential oil include the following steps: (1) Take fresh citrus peel or whole ylang-ylang plant, wash and dry it, then cut it into small pieces; (2) Add an ethanol-water solution to the small segment and mix well to obtain a mixture; the ethanol-water solution contains acetic acid; (3) Place the mixture into the extraction vessel, introduce supercritical carbon dioxide fluid for extraction, the extraction pressure is 25-35 MPa, the extraction temperature is 35-55℃, and the extraction time is 2-3 hours; (4) The extracted mixed fluid first enters the separation vessel I, with a separation pressure of 10-15 MPa and a separation temperature of 40-50℃, and then enters the separation vessel II, with a separation pressure of 8-9 MPa and a separation temperature of 30-40℃, to obtain citrus essential oil or ylang-ylang essential oil.

7. The method for preparing the pandan leaf aromatic product according to any one of claims 1 to 6, characterized in that, Includes the following steps: Mix all the ingredients and stir until homogeneous to obtain the pandan leaf aromatic product.

8. Application of compositions containing pandan leaf essential oil in the preparation of antidepressant products.

9. The application according to claim 8, characterized in that, The pandan leaf essential oil was obtained through supercritical carbon dioxide fluid extraction.

10. The application according to claim 8, characterized in that, The composition is selected from the pandan leaf aromatic products according to any one of claims 1 to 6.

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