Plate washer cleaning head and plate washer needle blocking detection method

Abstract

The invention provides a plate washing machine cleaning head and a plate washing machine needle blocking detection method.The plate washing machine cleaning head comprises a cleaning head body, a needle set and a detection assembly, a detection module is installed on the cleaning head body, the detection module is electrically connected with the needle set, and the detection module is used for detecting needle blocking when the needle set washes a microporous reaction plate. Liquid level characterization parameters of liquid in the microporous reaction plate are detected through a needle group; the plate washer needle blocking detection method adopts the plate washer cleaning head to carry out needle blocking detection. Due to the fact that the detection module can conduct needle blocking detection in real time in the microplate washing process, the situation that the microplate is washed when the needle set is in the needle blocking fault state can be avoided, a worker can eliminate the needle blocking fault in time, the needle set keeps the normal liquid injection and suction functions all the time, and the reliability and the washing effect of washing the microplate through the washing head are improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of medical devices, in particular to a washing head of a plate washer and a method for detecting a blocked needle of the plate washer. BACKGROUND

[0002] Enzyme-linked immunoassay is one of many clinical immunoassay techniques, and the washing of a micro-hole reaction plate is a key step in enzyme-linked immunoassay. In the washing step, repeated injection and suction of washing liquid is required to remove interfering substances remaining in the micro-holes. At present, a plate washer is usually used to clean the micro-hole reaction plate. During the washing process, if the plate washer is blocked, the liquid suction and injection of the plate washer will not be complete, which directly affects the washing quality. Therefore, it is necessary to detect the blocked needle of the plate washer.

[0003] In related technologies, a detection device is provided for detecting the blocked needle of the plate washer. The detection device is equipped with a washing liquid tank. During detection, the probe of the plate washer is inserted into the washing liquid tank, and whether the plate washer is blocked is determined according to the change of the liquid in the washing liquid tank. However, this detection method can only be used before and after the use of the plate washer, and cannot evaluate whether the plate washer is blocked during the washing process. Therefore, the washing effect of the plate washer on the micro-plate is still affected by the blocked needle during the washing process of the micro-plate. SUMMARY

[0004] The present application aims to at least solve one of the technical problems existing in the prior art. To this end, the present application provides a washing head of a plate washer, which can detect the blocked needle in real time when washing the micro-hole reaction plate, and improve the washing effect of the washing head of the plate washer on the micro-hole reaction plate.

[0005] According to the washing head of the plate washer provided by the first embodiment of the present application, the washing head of the plate washer comprises:

[0006] The washing head body is internally provided with a liquid suction channel and a liquid injection channel;

[0007] The needle group is connected to the liquid suction channel and the liquid injection channel, and can inject and suck liquid to the micro-hole reaction plate to wash the micro-hole reaction plate;

[0008] The detection assembly comprises a detection module, which is installed on the washing head body and electrically connected to the needle group. The detection module is used to detect the liquid level representation parameter of the liquid in the micro-hole reaction plate through the needle group when the needle group washes the micro-hole reaction plate.

[0009] The washing head of the plate washer according to the embodiment of the present application has at least the following beneficial effects:

[0010] The detection module in the application can detect the needle blockage in real time during the microplate washing process, avoids the needle group from washing the microplate in the fault state of needle blockage, and enables the staff to eliminate the needle blockage in time, so that the needle group always maintains the normal liquid injection and liquid suction function, and the reliability and washing effect of the washing head for washing the microplate are improved.

[0011] According to some embodiments of the application, the needle group comprises a liquid injection needle and a liquid suction needle, the liquid suction needle is in communication with the liquid suction channel, the liquid injection needle is in communication with the liquid injection channel, the liquid suction needle comprises a liquid suction end for sucking liquid in the micro-hole reaction plate, the liquid injection needle comprises a liquid injection end for injecting liquid into the micro-hole reaction plate, and the liquid suction end is lower than the liquid injection end.

[0012] The liquid suction needle is electrically connected with the detection module, the liquid injection needle is isolated from the detection module, and the detection module can detect the liquid level representation parameter of the liquid in the micro-hole reaction through the liquid suction needle.

[0013] According to some embodiments of the application, the liquid injection channel and the liquid suction channel are located on the same side of the detection module.

[0014] Alternatively, the liquid injection channel and the liquid suction channel are located on opposite sides of the detection module, respectively.

[0015] According to some embodiments of the application, the liquid injection channel and the liquid suction channel are located on the same side of the detection module, the detection module has a first through hole for the liquid suction needle to pass through, and the liquid suction needle is electrically connected with the inner wall of the first through hole.

[0016] The detection module also has a second through hole for the liquid injection needle to pass through, and there is a gap between the liquid injection needle and the inner wall of the second through hole; or the detection module is located on the side of the liquid injection needle, and there is a gap between the detection module and the liquid injection needle.

[0017] According to some embodiments of the application, the inside of the washing head body is provided with a mounting cavity, a first mounting channel and a second mounting channel, the two ends of the first mounting channel are in communication with the mounting cavity and the liquid suction channel respectively, and the two ends of the second mounting channel are in communication with the mounting cavity and the liquid injection channel respectively.

[0018] Part of the liquid suction needles are arranged in the first mounting cavity, part of the liquid injection needles are arranged in the second mounting cavity, and the detection module is accommodated in the mounting cavity.

[0019] According to some embodiments of the application, the plate washing machine washing head comprises a plurality of needle groups, and each needle group comprises a liquid injection needle and a liquid suction needle.

[0020] The liquid suction channel comprises a main flow channel, a plurality of branch flow channels and a plurality of liquid suction branches. The branch flow channels are arranged at intervals along the extension direction of the main flow channel. One end of each branch flow channel is in communication with the main flow channel, and the other end of each branch flow channel is in communication with at least one liquid suction branch. The end of the liquid suction branch forms a liquid suction port. Each liquid suction port is in communication with one liquid suction needle. The liquid suction ports are arranged along a first direction and / or a second direction. The first direction is parallel to the extension direction of the main flow channel. The first direction intersects the second direction.

[0021] According to some embodiments of the present application, the plate washer cleaning head has a first working state, a second working state and a third working state. When the plate washer cleaning head is in the first working state, the needle group performs one of a liquid suction action, a liquid injection action and a position switching action. When the plate washer cleaning head is in the second working state, the detection module performs a re-inspection action. When the plate washer cleaning head is in the third working state, the plate washer cleaning head alarms.

[0022] The detection assembly further comprises a control module in communication connection with the detection module. The control module is configured to switch the plate washer cleaning head between the first working state, the second working state and the third working state according to the detection result of the detection module.

[0023] According to the plate washer needle blocking detection method provided by the second embodiment of the present application, the plate washer cleaning head in the first embodiment is used for needle blocking detection, which comprises the following steps:

[0024] When the micro-hole reaction plate is washed, the needle group performs a liquid suction action and a liquid injection action on the micro-hole reaction plate.

[0025] The detection module detects a liquid level representation parameter H1 of the liquid in the micro-hole reaction plate when the needle group completes the liquid suction action, and a liquid level representation parameter H2 of the liquid in the micro-hole reaction plate when the needle group completes the liquid injection action.

[0026] When H1 is greater than a first preset value, it is determined that the needle group is blocked. When H2 is less than a second preset value, it is determined that the needle group is blocked.

[0027] According to some embodiments of the present application, the needle group comprises a liquid injection needle and a liquid suction needle. The liquid injection needle is used to perform the liquid injection action, and the liquid suction needle is used to perform the liquid suction action. The liquid suction needle is electrically connected with the detection module, and the liquid injection needle is isolated from the detection module. The plate washer needle blocking detection method comprises the following steps:

[0028] After the liquid suction needle completes the liquid suction action, the detection module measures a liquid level representation parameter H1 through the liquid suction needle; after the liquid injection needle completes the liquid injection action, the detection module measures a liquid level representation parameter H2 through the liquid suction needle.

[0029] According to some embodiments of the present application, the liquid level representation parameter H1 and the liquid level representation parameter H2 are set as the capacitance value of the liquid suction needle detected by the detection module; the plate washer needle blocking detection method comprises:

[0030] Before the liquid suction needle performs the liquid suction action, the detection module obtains a capacitance value C0 through the liquid suction needle;

[0031] After the liquid suction needle completes the liquid suction action, the detection module obtains a capacitance value C1 through the liquid suction needle; if the result of C1-C0 is greater than a first preset difference value, it is determined that the liquid suction needle is blocked;

[0032] After the liquid injection needle completes the liquid injection action, the detection module obtains a capacitance value C2 through the liquid suction needle; if the result of C2-C1 is less than a second preset difference value, it is determined that the liquid injection needle is blocked.

[0033] According to some embodiments of the present application, the plate washer needle blocking detection method comprises:

[0034] After it is determined that the liquid suction needle is blocked, the detection module at least performs n times of actions of obtaining the capacitance value C1 through the liquid suction needle;

[0035] If the result of C1-C0 is greater than a first preset difference value, the plate washer cleaning head alarms;

[0036] If the result of C1-C0 is less than a first preset difference value, the determination result of the liquid suction needle being blocked is eliminated, or the liquid injection needle performs the liquid injection action; and / or,

[0037] After it is determined that the liquid injection needle is blocked, the plate washer cleaning head alarms.

[0038] According to some embodiments of the present application, the plate washer needle blocking detection method comprises:

[0039] When the microwell reaction plate is washed, the liquid suction needle and the liquid injection needle are synchronously raised or lowered, and the liquid suction needle continuously maintains the liquid suction state;

[0040] The needle group has an initial height; when the needle group is at the initial height, the detection module obtains the capacitance value C0 through the liquid suction needle;

[0041] When the needle group drops to a first preset height, the liquid suction needle performs the liquid suction action and sucks liquid in the microwell reaction plate; after the liquid suction needle completes the liquid suction action, the detection module performs a needle blockage detection action on the liquid suction needle and obtains the capacitance value C1;

[0042] If the detection result is that the liquid suction needle is not blocked, the needle group rises to a second preset height, the liquid injection needle starts to perform the liquid injection action and injects liquid into the microwell reaction plate; after the liquid injection needle completes the liquid injection action, the detection module performs a needle blockage detection action on the liquid injection needle and obtains the capacitance value C2, and if the needle blockage detection result is that the liquid injection needle is not blocked.

[0043] Other features and advantages of the present application will be set forth in the following description, and in part will become apparent to those skilled in the art from the description, or can be learned by practice of the application. BRIEF DESCRIPTION OF DRAWINGS

[0044] The present application will be further described below in conjunction with the accompanying drawings and examples, in which:

[0045] Figure 1 An exploded schematic view of a cleaning head of a plate washer provided by the present application;

[0046] Figure 2 A schematic view of the cooperation between a needle group and a mounting channel in the cleaning head of the plate washer and a schematic view of a liquid suction channel;

[0047] Figure 3 A schematic view of the cooperation between a liquid suction channel, a liquid injection channel, a detection module and a needle group in an embodiment;

[0048] Figure 4 A schematic view of the cooperation between a liquid suction channel, a liquid injection channel, a detection module and a needle group in another embodiment;

[0049] Figure 5 A schematic view of the positions of a liquid suction needle, a liquid injection needle and a detection module in an embodiment;

[0050] Figure 6 A schematic view of the positions of a liquid suction needle, a liquid injection needle and a detection module in another embodiment;

[0051] Figure 7 A schematic view of the positions of a liquid suction needle, a liquid injection needle and a detection module in still another embodiment;

[0052] Figure 8 A schematic view of the positions of a liquid suction needle, a liquid injection needle and a detection module in yet another embodiment;

[0053] Figure 9 A sectional view of a cross section of the cleaning head of the plate washer passing through a liquid injection channel;

[0054] Figure 10 Another sectional view of the cleaning head section passing through the liquid suction passage for the plate washer.

[0055] Reference signs:

[0056] Cleaning head body 100, liquid suction passage 110, main flow channel 111, branch flow channel 112, liquid suction branch 113, liquid suction port 114, liquid injection passage 120, mounting cavity 130, first mounting passage 140, second mounting passage 150, threading hole 160, wire pressing plate 170, liquid suction interface 180, liquid injection interface 190, sealing silica gel 101, cover plate 102;

[0057] Needle group 200, liquid injection needle 210, liquid injection end 211, liquid suction needle 220, liquid suction end 221;

[0058] Detection assembly 300, detection module 310, first through hole 311, second through hole 312, control module 320. DETAILED DESCRIPTION

[0059] The embodiments of the present application will be described in detail below, examples of which are shown in the drawings, wherein the same or similar notations represent the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the drawings are exemplary only, for the purpose of explaining the present application, and should not be understood as a limitation of the present application.

[0060] In the description of the present application, it should be understood that the orientation description, such as the orientation or position relationship indicated by up, down, etc. is based on the orientation or position relationship shown in the drawings, and is only for the purpose of facilitating the description of the present application and simplifying the description, and therefore cannot be understood as indicating or implying that the device or element indicated must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation of the present application.

[0061] In the description of the present application, the plural refers to two or more. If there is a description of first, second, etc., it is only for the purpose of distinguishing technical features, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of indicated technical features or implicitly indicating the sequence of indicated technical features.

[0062] In the description of the present application, unless otherwise explicitly limited, the words such as setting, mounting, connecting, etc. should be broadly understood, and the person skilled in the art can reasonably determine the specific meaning of the above words in the present application in combination with the specific content of the technical solution.

[0063] The technical solutions of the present application will be described below in conjunction with the drawings. Obviously, the following described embodiments are part of the embodiments of the present application, not all embodiments.

[0064] During the washing process of the micro-hole reaction plate by the plate washer, the plate washer needs to repeatedly add and suck and discard the washing liquid. However, due to the influence of the debris in the micro-hole reaction plate and the impurities in the sample, the plate washer is prone to needle blockage, which leads to the situations of no addition, insufficient addition, no suction, and incomplete suction during the washing process, directly affecting the washing quality of the micro-hole reaction plate. In some existing methods, a detection device for detecting the needle blockage of the plate washer is arranged, but the detection device needs to be additionally provided with a washing liquid tank, and the probe of the plate washer is inserted into the washing liquid tank during detection, and whether the plate washer is blocked is judged according to the liquid change in the washing liquid tank. However, this detection method can only be detected before and after the use of the plate washer, and cannot evaluate whether the plate washer is blocked during the washing process. Therefore, the washing effect of the plate washer on the micro plate is still affected by the needle blockage during the washing process of the micro plate.

[0065] In view of the above situation, the present application provides a plate washer cleaning head (hereinafter referred to as cleaning head), which can detect the needle blockage in real time during the washing process of the micro-hole reaction plate (hereinafter referred to as micro plate), so as to improve the washing quality of the cleaning head on the micro plate.

[0066] Reference Figure 1 The cleaning head includes a cleaning head body 100, a needle group 200, and a detection assembly 300. The inside of the cleaning head body 100 is provided with a liquid suction channel 110 and a liquid injection channel 120. The needle group 200 communicates with the liquid suction channel 110 and the liquid injection channel 120. The liquid injection channel 120 can be externally connected to an input pipeline. The input pipeline introduces the washing liquid into the liquid suction channel 110, and then the needle group 200 adds the washing liquid to the micro plate. The liquid suction channel 110 is externally connected to an output pipeline. The washing liquid in the micro plate sucked by the needle group 200 enters the output pipeline through the liquid suction channel 110, and then is discharged from the output pipeline.

[0067] The micro plate is usually provided with a plurality of reaction cups. During the washing of the micro plate by the cleaning head, the needle group 200 alternately sucks and injects liquid to the micro plate. During the liquid injection process of the needle group 200, the washing liquid is injected into the reaction cup of the micro plate, and the washing liquid cleans the micro plate. During the liquid suction process of the needle group 200, the liquid in the reaction cup of the micro plate is sucked out. After multiple liquid suction and injection, the washing of the micro plate is realized.

[0068] The detection assembly 300 is used for needle blocking detection of the needle group 200 in the microplate washing process. Specifically, the detection assembly 300 includes a detection module 310, which is installed on the cleaning head body 100, and the detection module 310 is electrically connected with the needle group 200, so that the detection module 310 and the needle group 200 are electrically connected and information interaction is realized. Since the needle group 200 and the detection module 310 are both installed on the cleaning head body 100, when the needle group 200 washes the microplate, the detection module 310 can also detect the liquid level representation parameter of the liquid in the microplate (which can be the liquid level representation parameter of the liquid in the reaction cup) by using the needle group 200, so that real-time needle blocking detection is realized in the microplate washing process, and the needle group 200 is prevented from being blocked in the microplate washing process, so that the normal liquid injection and liquid suction are affected, and the washing quality of the microplate is affected.

[0069] The detection module 310 is configured to form a device with liquid level detection function with the needle group 200. For example, the detection module 310 is configured as a sensor with detection function, a circuit board with integrated detection circuit, etc. Since the detection module 310 detects the liquid level in the microplate by using the needle group 200, when the needle group 200 performs liquid injection and liquid suction, the needle group 200 is close to the liquid in the microplate, and the detection of the liquid level in the microplate by the detection module 310 is more convenient. The liquid level representation parameter is used to represent the liquid level in the microplate. When the detection module 310 uses different detection methods for liquid level detection, the type of the liquid level representation parameter is different. The detection method of the detection module 310 for the liquid level is not limited to capacitive liquid level detection, resistive liquid level detection, ultrasonic liquid level detection, etc. For example, for capacitive liquid level detection, the needle group 200 is usually configured as a hollow conductor to facilitate the passage of washing liquid. When the needle group 200 contacts the liquid in the microplate, the liquid fills in the needle group 200, which is equivalent to form a capacitor. When the liquid amount in the needle group 200 changes, the capacitance value changes accordingly. Therefore, the capacitance value of the needle group 200 measured by the detection module 310 can represent the liquid level in the microplate, and the liquid level representation parameter is the capacitance value measured by the detection module 310. Of course, when the detection module 310 uses resistive liquid level detection, the liquid level representation parameter is the resistance value measured by the detection module 310. When the detection module 310 uses ultrasonic liquid level detection, the liquid level representation parameter is the time value measured by the detection module 310.

[0070] It can be understood that when the needle group 200 completes the liquid suction action, if the needle group 200 is not blocked, the liquid level characteristic parameter of the washing liquid in the microplate should be lower than the first preset value. If the liquid level characteristic parameter detected by the detection module 310 exceeds the first preset value, it means that the needle group 200 is blocked. At this time, the staff can replace and maintain the blocked needle group 200 to restore the liquid suction function of the needle group 200. Similarly, when the needle group 200 completes the liquid injection action, if the needle group 200 is not blocked, the liquid level characteristic parameter of the washing liquid in the reaction cup of the microplate should be higher than the second preset value. If the liquid level characteristic parameter detected by the detection module 310 does not reach the second preset value, it means that the needle group 200 is blocked. At this time, the staff can replace and maintain the blocked needle group 200 to restore the liquid injection function of the needle group 200.

[0071] It should be noted that the second preset value is set to be lower than the full load liquid level (the liquid level of the liquid when the reaction cup is full of liquid) of the reaction cup. Similarly, the first preset value can be the empty load liquid level (i.e. the liquid level of the liquid when the liquid in the reaction cup is sucked out) of the reaction cup, or can be set to be higher than the empty load liquid level of the reaction cup according to the washing demand of the microplate. For example, for the case that the first preset value is set to be less than the full load liquid level and the first preset value is set to be the empty load liquid level, when the needle group 200 completes the liquid injection action to the reaction cup, the reaction cup is full of washing liquid, and when the needle group 200 completes the liquid suction action to the reaction cup, the liquid in the reaction cup is sucked out. In this case, the washing of the microplate is more thorough, which is beneficial to improve the washing quality of the microplate.

[0072] Since the detection module 310 in the application can detect the blockage of the needle group 200 in real time during the washing of the microplate, the needle group 200 in the fault state of blockage is avoided to wash the microplate, thereby causing the situations of no injection, incomplete injection, no liquid suction or incomplete liquid suction in the washing process, and directly affecting the washing effect of the microplate. The application detects the blockage of the needle group 200 in real time. When the needle group 200 is blocked, the staff can replace and maintain the needle group 200 in time to eliminate the blockage fault, so that the needle group 200 always maintains the normal liquid injection and suction function, and improves the reliability and washing effect of the cleaning head in washing the microplate.

[0073] The needle group 200 generally comprises a liquid injection needle 210 and a liquid suction needle 220, the liquid suction needle 220 is arranged side by side with the liquid injection needle 210, the liquid injection needle 210 is communicated with the liquid injection channel 120 in the cleaning head main body 100 and is used for injecting liquid into the reaction cup of the microplate, and the liquid suction needle 220 is communicated with the liquid suction channel 110 in the cleaning head main body 100 and is used for sucking liquid in the reaction cup of the microplate; that is, when the needle group 200 washes the microplate, the liquid suction needle 220 performs a liquid suction action, and the liquid injection needle 210 performs a liquid injection action. The liquid suction action of the liquid suction needle 220 and the liquid injection action of the liquid injection needle 210 are alternately performed in sequence and repeatedly, and the washing of the microplate is completed through multiple liquid injection and suction.

[0074] In some embodiments, the detection module 310 detects the liquid level representation parameter of the liquid in the microplate through the liquid suction needle 220 to detect whether the liquid suction needle 220 is blocked, and detects the liquid level representation parameter of the liquid in the microplate through the liquid injection needle 210 to detect whether the liquid injection needle 210 is blocked. Taking the capacitive liquid level detection as an example, the liquid level representation parameter is the capacitance value detected by the detection module 310. After the liquid injection needle 210 completes the liquid injection action, the detection module 310 detects the capacitance value of the liquid injection needle 210. If the capacitance value of the liquid injection needle 210 is greater than a set value, it indicates that the liquid injection amount of the liquid injection needle 210 reaches the set amount, and if the capacitance value of the liquid injection needle 210 is less than the set value, it indicates that the liquid injection amount of the liquid injection needle 210 does not reach the set amount, and the liquid injection needle 210 has a risk of being blocked. Similarly, after the liquid suction needle 220 completes the liquid suction action, the detection module 310 detects the capacitance value of the liquid suction needle 220. If the capacitance value of the liquid suction needle 220 is less than the set value, it indicates that the liquid suction amount of the liquid suction needle 220 reaches the set amount, and the liquid suction of the liquid suction needle 220 is smooth, and if the capacitance value of the liquid suction needle 220 is greater than the set value, it indicates that the liquid suction amount of the liquid suction needle 220 does not reach the set amount, and the liquid suction needle 220 has a risk of being blocked. In this way, the detection module 310 detects the liquid level of the microplate by using the liquid suction needle 220 and the liquid injection needle 210 respectively, so as to determine whether the liquid suction needle 220 and the liquid injection needle 210 are blocked.

[0075] It should be noted that the above-mentioned set value can be set to a specific numerical value, can also be set to a numerical range, and can also be a value determined according to the capacitance value detected by the liquid suction needle 220 in a certain state during the liquid injection and suction process. For example, during the liquid injection process, the set value is determined according to the capacitance value detected when the liquid injection needle 210 is located in the reaction cup and has not started liquid injection (for example, the capacitance value in this state is -2000); during the liquid suction process, the set value is determined according to the capacitance value detected when the liquid injection needle 210 is located above the reaction cup and does not contact the liquid (for example, the capacitance value in this state is +2000). In this way, the set value is determined according to the actual liquid suction capacity of the liquid suction needle 220 and the actual liquid injection capacity of the liquid injection needle 210 in the current liquid injection and suction stage, so as to reduce the influence of the liquid, impurities and other residues in the liquid suction needle 220 and the liquid injection needle 210 on the precision of the blockage detection and the probability of false judgment.

[0076] In another embodiment, the detection module 310 only detects the clogging of the needle set 200 through one of the liquid injection needle 210 or the liquid suction needle 220. Taking the detection module 310 detecting the clogging of the needle set 200 through the liquid suction needle 220 as an example, specifically, the liquid suction needle 220 includes a liquid suction end 221 for sucking the liquid in the microplate, the liquid injection needle 210 includes a liquid injection end 211 for injecting liquid into the microplate, the liquid suction end 221 is located at the lower end of the liquid suction needle 220, the liquid injection end 211 is located at the lower end of the liquid injection needle 210, and the liquid suction end 221 is lower than the liquid injection end 211. Thus, since the needle set 200 can be translated or lifted synchronously with the cleaning head, the liquid suction needle 220 is electrically connected with the detection module 310, and the liquid injection needle 210 is isolated from the detection module 310. When the cleaning head is lowered towards the microplate, the liquid suction needle 220 approaches the microplate first and contacts the liquid in the microplate, and the detection module 310 can directly detect the liquid level characterization parameter by using the liquid suction needle 220. The liquid injection needle 210 does not contact the liquid in the microplate, and the detection module 310 detects the liquid level characterization parameter by using the liquid suction needle 220 is not affected by the liquid injection needle 210.

[0077] For example, taking the capacitive liquid level detection as an example, when the detection module 310 detects the liquid level characterization parameter by using the liquid suction needle 220, after the liquid suction needle 220 completes the liquid suction action, the detection module 310 detects the capacitance value of the liquid suction needle 220. When the capacitance value of the liquid suction needle 220 is less than a set value, it indicates that the liquid suction amount of the liquid suction needle 220 reaches the set amount, and the liquid suction needle 220 sucks smoothly. When the capacitance value of the liquid suction needle 220 is greater than the set value, it indicates that the liquid in the microplate is not completely sucked, the liquid suction amount of the liquid suction needle 220 does not reach the set amount, and the liquid suction needle 220 has a risk of clogging. After the liquid injection needle 210 completes the liquid injection action, the detection module 310 detects the capacitance value of the liquid suction needle 220. When the capacitance value of the liquid suction needle 220 is greater than the set value, it indicates that the liquid injection amount of the liquid injection needle 210 into the microplate reaches the set amount, and the liquid injection needle 210 injects smoothly. When the capacitance value of the liquid suction needle 220 is less than the set value, it indicates that the liquid injection amount of the liquid injection needle 210 into the microplate does not reach the set amount, and the liquid injection needle 210 has a risk of clogging. It should be noted that the set value can be set to a specific value, or can be set to a range of values.

[0078] The detection assembly 300 further includes a control module 320, which is communicatively connected with the detection module 310. The control module 320 is electrically connected with the detection module 310 and can exchange information with the detection module 310. The communication connection mode is not limited to wire connection, Bluetooth, WiFi, infrared, etc. The cleaning head has multiple working states, and the control module 320 can switch the cleaning head between different working states. The control module 320 can be integrated into a control system such as an industrial computer or a workstation.

[0079] Specifically, the cleaning head has a first working state, a second working state and a third working state, and the control module 320 can switch the cleaning head between the first working state, the second working state and the third working state according to the detection result of the detection module 310. When the cleaning head is in the first working state, the needle group 200 performs one of the liquid injection action, the liquid suction action and the position switching. For example, when the needle group 200 performs the liquid injection action, the liquid injection needle 210 injects liquid into the reaction cup of the microplate; when the needle group 200 performs the liquid suction action, the liquid suction needle 220 sucks the liquid in the reaction cup of the microplate; and when the needle group 200 performs the position switching, the needle group 200 is lifted or translated. When the needle group 200 is lifted, the liquid injection needle 210 or the liquid suction needle 220 can be close to or away from the reaction cup of the microplate, so as to facilitate the needle group 200 to perform the liquid injection and suction actions. When the needle group 200 is translated, it can be moved to different positions of the microplate, and then the liquid injection and suction actions can be performed on the reaction cups in other areas of the microplate. When the cleaning head is in the first working state, the microplate is washed. During this process, the control module 320 controls the needle group 200 to repeatedly inject and suck liquid into the reaction cup of the microplate, and through the position switching, the needle group 200 can be close to or away from the reaction cup and be switched to the reaction cup at different positions to suck liquid, thereby completing the washing of the entire microplate.

[0080] When the needle group 200 completes the liquid injection action or the liquid suction action, the control module 320 controls the detection module 310 to perform the needle blocking detection. If the detection module 310 detects that the needle group 200 is blocked, the control module 320 controls the cleaning head to switch to the second working state. When the cleaning head is in the second working state, the detection module 310 performs the re-detection action, i.e., after the detection module 310 first determines that the needle group 200 is blocked, the detection module 310 re-detects the determination result at least once to ensure the correctness of the detection result. For example, if the detection module 310 determines that the liquid suction needle 220 is blocked after the first liquid level detection, the control module 320 controls the detection module 310 to perform the liquid level detection action again and determine whether the liquid suction needle 220 is blocked again, so as to reduce the determination error through the re-detection and reduce the probability of misjudgment caused by bubbles, impurities and other interference factors.

[0081] When the needle group 200 completes the liquid injection action or the liquid suction action, the control module 320 controls the detection module 310 to perform the needle blocking detection. If the detection module 310 detects that the needle group 200 is blocked, the control module 320 controls the cleaning head to switch to the third working state. When the cleaning head is in the third working state, the cleaning head alarms. The alarm mode can be set to at least one of buzzing, playing the fault type, flashing the alarm light, so as to prompt the staff to troubleshoot and eliminate the needle blocking fault.

[0082] It should be noted that the third working state can be arranged after the liquid injection action and the liquid suction action in the first working state, or can be arranged after the second working state. For example, when the detection module 310 determines that the liquid injection needle 210 is blocked, the control module 320 controls the cleaning head to switch to the third working state, so that the cleaning head alarms and reminds the staff to troubleshoot the blockage of the liquid injection needle 210; or when the detection module 310 determines that the liquid suction needle 220 is blocked, the control module 320 controls the cleaning head to switch to the second working state, so that the detection module 310 performs a re-detection action to detect whether the liquid suction needle 220 is blocked again. If the re-detection result is that the liquid suction needle 220 is blocked, the control module 320 controls the cleaning head to switch to the third working state, so that the cleaning head alarms and reminds the staff to troubleshoot the blockage of the liquid suction needle 220.

[0083] For the case where the detection module 310 only uses the liquid suction needle 220 to detect the blockage, the detection module 310 only interacts with the liquid suction needle 220, so the liquid suction needle 220 needs to be electrically connected to the detection module 310 and in communication with the liquid suction channel 110, and the liquid injection needle 210 needs to be isolated from the detection module 310 and in communication with the liquid injection channel 120. Based on this, in an embodiment, as shown in Figure 2 , the liquid injection channel 120 and the liquid suction channel 110 are located on the same side of the detection module 310, and both the liquid injection channel 120 and the liquid suction channel 110 are located above the detection module 310. The upper end of the liquid suction needle 220 is in communication with the liquid injection channel 120, the liquid suction needle 220 penetrates through the detection module 310 and is connected to the detection module 310, and the upper end of the liquid injection needle 210 is in communication with the liquid injection channel 120. The detection module 310 avoids the liquid injection channel 120 and the liquid injection needle 210. In this case, since the liquid injection channel 120 and the liquid suction channel 110 are both located above the cleaning head body, the flow channel is concentrated in the upper part of the cleaning head, and the electrical elements and lines are concentrated in the lower part of the cleaning head body, so the flow channel and the lines are more convenient to arrange.

[0084] In another embodiment, as shown in Figure 3 , the liquid injection channel 120 and the liquid suction channel 110 are located on the same side of the detection module 310, and both the liquid injection channel 120 and the liquid suction channel 110 are located below the detection module 310. The upper end of the liquid suction needle 220 is connected to the detection module 310, the liquid suction channel 110 is located between the detection module 310 and the liquid injection channel 120 and is in communication with the liquid suction needle 220, the upper end of the liquid injection needle 210 is in communication with the liquid injection channel 120, and the liquid injection needle 210 is located below the detection module 310 as a whole, so that the liquid injection needle 210 is isolated from the detection module 310.

[0085] Alternatively, in another embodiment, as shown in Figure 4As shown, the liquid injection channel 120 and the liquid suction channel 110 are located on opposite sides of the detection module 310, the liquid suction channel 110 is located above the detection module 310, and the liquid injection channel 120 is located below the detection module 310. The upper end of the liquid suction needle 220 is in communication with the liquid suction channel 110, and the upper end of the liquid injection needle 210 is in communication with the liquid injection channel 120. The liquid injection needle 210 is located below the detection module 310 as a whole, so that the liquid injection needle 210 is isolated from the detection module 310. The liquid suction needle 220 penetrates through the detection module 310 and is connected to the detection module 310.

[0086] The connection mode of the liquid suction needle 220 and the detection module 310 is not limited. The liquid suction needle 220 can be welded to the detection module 310, or the liquid suction needle 220 can be connected to the detection module 310 through a wire, or the detection module 310 can be provided with a through hole for the liquid suction needle 220 to penetrate through, and the circumferential side of the liquid suction needle 220 can be in contact with the detection module 310 to realize electrical conduction.

[0087] For the avoidance mode of the detection module 310 to the liquid injection needle 210, in an embodiment, the detection module 310 has a through hole for the liquid injection needle 210 to penetrate through. There is a gap between the inner wall of the through hole and the liquid injection needle 210. The liquid injection needle 210 is not in contact with the detection module 310, so that the detection module 310 avoids the liquid injection needle 210. Alternatively, in another embodiment, the liquid injection needle 210 is located on the side of the detection module 310 as a whole. There is a gap between the detection module 310 and the liquid injection needle 210, so that the detection module 310 avoids the liquid injection needle 210.

[0088] Specifically, as shown in the embodiment shown in Figure 1 and Figure 5 , the liquid injection channel 120 and the liquid suction channel 110 are located on the same side of the detection module 310. The liquid injection channel 120 and the liquid suction channel 110 are both located above the detection module 310. The upper end of the liquid suction needle 220 is in communication with the liquid injection channel 120, and the upper end of the liquid injection needle 210 is in communication with the liquid injection channel 120. The detection module 310 has a first through hole 311 for the liquid suction needle 220 to penetrate through. The liquid suction needle 220 is electrically connected to the inner wall of the first through hole 311. The detection module 310 has a second through hole 312 for the liquid injection needle 210 to penetrate through. There is a gap between the liquid injection needle 210 and the inner wall of the second through hole 312. The electrical connection between the liquid suction needle 220 and the detection module 310 is realized, and the liquid injection needle 210 is isolated from the detection module 310.

[0089] As shown in Figure 1 , Fig. 6, and Figure 7In the shown embodiment, the liquid injection channel 120 and the liquid suction channel 110 are located on the same side of the detection module 310, and the liquid injection channel 120 and the liquid suction channel 110 are both located above the detection module 310. The upper end of the liquid suction needle 220 is in communication with the liquid injection channel 120, and the upper end of the liquid injection needle 210 is in communication with the liquid injection channel 120. The detection module 310 has a first through hole 311 through which the liquid suction needle 220 passes. The liquid suction needle 220 is electrically connected to the inner wall of the first through hole 311. The liquid injection needle 210 is located on the side of the detection module 310 and has a gap between the liquid injection needle 210 and the detection module 310. The liquid suction needle 220 is electrically connected to the detection module 310, and the liquid injection needle 210 is isolated from the detection module 310. As shown in Figure 6 The detection module 310 is at least partially in the shape of a U. Each needle group 200 includes a liquid injection needle 210 and a liquid suction needle 220. The arrangement direction of the liquid injection needle 210 and the liquid suction needle 220 in each needle group 200 is the same. As shown in Figure 7 The detection module 310 is at least partially in the shape of a strip. The liquid suction needles 220 in adjacent needle groups 200 are arranged towards each other. The liquid injection needles 210 are located on the outside of the detection module 310.

[0090] In other embodiments, as shown in Figure 8 The liquid injection needle 210 in part of the needle groups 200 can pass through a second through hole 312 of the detection module 310 and have a gap between the liquid injection needle 210 and the second through hole 312. The liquid injection needle 210 in part of the needle groups 200 is located on the side of the detection module 310 and has a gap between the liquid injection needle 210 and the detection module 310. In this way, the liquid injection needle 210 in all needle groups 200 is isolated from the detection module 310.

[0091] Referring to Figure 2 , Figure 9 and Figure 10The inside of the cleaning head body 100 is provided with a mounting cavity 130 for accommodating the detection module 310, and is also provided with a first mounting channel 140 for accommodating the liquid suction needle 220 and a second mounting channel 150 for accommodating the liquid injection needle 210. The first mounting channel 140 is in communication with the mounting cavity 130 and the liquid suction channel 110, and the second mounting channel 150 is in communication with the mounting cavity 130 and the liquid injection channel 120. Part of the liquid suction needle 220 is arranged in the first mounting cavity 130, and part of the liquid injection needle 210 is arranged in the second mounting cavity 130. Specifically, the upper end of the liquid suction needle 220 is in communication with the liquid suction channel 110 through the first mounting channel 140, the liquid suction needle 220 penetrates the detection module 310 arranged in the mounting cavity 130, and the lower end of the liquid suction needle 220 penetrates out of the outside of the first mounting channel 140 and forms a liquid suction end 221. The upper end of the liquid injection needle 210 is in communication with the liquid injection channel 120, and the lower end of the liquid injection needle 210 penetrates out of the outside of the second mounting channel 150 and forms a liquid injection end 211. The detection module 310 is arranged inside the cleaning head body 100, which on the one hand protects the detection module 310 and isolates it from the outside environment, reducing the influence of the external environment on the detection module 310, and on the other hand facilitates the connection of the detection module 310 and the liquid suction needle 220. The liquid suction needle 220 penetrates the detection module 310 to realize electrical conduction between the two, and the assembly is more convenient.

[0092] Further, the connection mode of the detection module 310 and the cleaning head body 100 is not limited to the threaded connection of the detection module 310 and the cleaning head body 100, so that the detection module 310 is stable in the mounting cavity 130. Further, when the detection module 310 is installed in the cleaning head body 100, the detection module 310 is first arranged in the mounting cavity 130, and then the threaded fastener is locked between the detection module 310 and the cleaning head body 100, so that the detection module 310 is locked in the mounting cavity 130. Then, the mounting cavity 130 is filled with glue, and the detection module 310 is sealed in the mounting cavity 130, improving the isolation effect between the detection module 310 and the external environment, avoiding the entry of water vapor, impurities and the like into the mounting cavity 130.

[0093] The cleaning head body 100 is also provided with a wire hole 160 in communication with the mounting cavity 130. A wire can be arranged in the wire hole 160. One end of the wire is inserted into the mounting cavity 130 and connected to the detection module 310, and the other end is connected to the control module 320 to realize communication connection between the detection module 310 and the control module 320. Figure 1In the shown embodiment, the threading hole 160 is arranged at the bottom of the cleaning head body 100, and the lower end of the threading hole 160 is provided with an opening, through which the wire can be placed into the threading hole 160, so that the connection of the wire is more convenient; the cleaning head further comprises a wire pressing plate 170 connected to the bottom of the cleaning head body 100, which covers the opening of the threading hole 160 to restrict the wire in the threading hole 160, and the connection mode of the wire pressing plate 170 and the cleaning head body 100 is not limited to threaded connection, clamping, bonding, etc.

[0094] It should be noted that the cleaning head usually comprises a plurality of needle groups 200, and when washing the microplate, the liquid suction needle 220 and the liquid injection needle 210 in each needle group 200 suck and inject liquid into the same reaction cup of the microplate, and the liquid suction needles 220 in the plurality of needle groups 200 simultaneously perform the liquid suction action, and the liquid injection needles 210 simultaneously perform the liquid injection action, so that the plurality of needle groups 200 can simultaneously suck and inject liquid into a plurality of reaction cups in the microplate. In an embodiment, the plurality of needle groups 200 are arranged in multiple rows and multiple columns, and the spacing between adjacent needle groups 200 and the arrangement mode of the needle groups 200 match the arrangement mode of the reaction cups in the microplate.

[0095] In an embodiment, referring to Figure 2 , the liquid suction channel 110 comprises a main flow channel 111, a plurality of branch flow channels 112, and a plurality of liquid suction branches 113, the branch flow channels 112 are arranged at intervals along the extension direction of the main flow channel 111, one end of each branch flow channel 112 is in communication with the main flow channel 111, and the other end is in communication with at least one liquid suction branch 113, the end of the liquid suction branch 113 forms a liquid suction port 114, each liquid suction port 114 is in communication with one liquid suction needle 220, and the liquid suction ports 114 are arranged along the first direction and / or the second direction, the first direction is parallel to the extension direction of the main flow channel 111, the first direction intersects the second direction, and in some embodiments, the first direction can be perpendicular to the second direction. The liquid sucked by each liquid suction needle 220 enters the liquid suction branch 113, and then flows into the branch flow channel 112 through different liquid suction branches 113, the liquid in different branch flow channels 112 flows to the main flow channel 111, and then is discharged from the cleaning head body 100 through the main flow channel 111; referring to Figure 1 , the cleaning head body 100 is provided with a liquid suction interface 180 and a liquid injection interface 190, the liquid suction interface 180 is in communication with the main flow channel 111, the liquid suction interface 180 is connected to a negative pressure source to provide a liquid suction force, and the liquid injection interface 190 is connected to a liquid supply pipe to input washing liquid into the liquid injection channel 120.

[0096] It can be understood that, due to the multi-stage shunt provided by the liquid suction channel 110, the suction force in the main flow channel 111 is uniformly diffused into different liquid suction branches 113, so that the suction force of each liquid suction needle 220 is uniform; on the one hand, when the needle group 200 washes the microplate, the suction force of the liquid suction needle 220 in each needle group 200 is uniform, so that each liquid suction needle 220 can suck the liquid in the different reaction cups of the microplate with a more balanced suction force, reducing the probability of incomplete liquid suction caused by insufficient suction force of the liquid suction needle 220, and helping to improve the washing effect of the microplate; on the other hand, since the suction force of each liquid suction needle 220 is uniform, the probability of uneven liquid amount in the reaction cup of the microplate after the liquid suction needle 220 sucks liquid due to the large suction force of some liquid suction needles 220 and the small suction force of some liquid suction needles 220 can be reduced, thereby reducing the misjudgment probability of the needle blocking detection of the detection module 310 when detecting the liquid level of the microplate through the liquid suction needle 220, and improving the needle blocking detection precision.

[0097] Referring to Figure 1 The upper side of the liquid suction channel 110 is provided with a sealing silica gel 101, the sealing silica gel 101 seals the liquid suction channel 110, and the upper side of the sealing silica gel 101 is provided with a cover plate 102, the cover plate 102 covers and seals the sealing silica gel 101, and is fixed to the top of the cleaning head main body 100, so as to fix the sealing silica gel 101 and optimize the sealing of the liquid suction channel 110 by the sealing silica gel 101.

[0098] The application also provides a plate washing machine needle blocking detection method (hereinafter referred to as needle blocking detection method), and the above-mentioned cleaning head is used for needle blocking detection. The needle blocking detection method comprises:

[0099] When the cleaning head washes the microplate, the needle group 200 performs liquid injection and liquid suction actions on the microplate, the needle group 200 injects washing liquid into the reaction cup of the microplate when performing the liquid injection action, and the needle group 200 sucks the liquid in the reaction cup of the microplate when performing the liquid suction action; when the cleaning head washes the microplate, the detection module 310 detects the liquid level representation parameter H1 of the liquid in the microplate when the needle group 200 completes the liquid suction action, and detects the liquid level representation parameter H2 of the liquid in the microplate after the needle group 200 completes the liquid injection action, both H1 and H2 can indicate the liquid level representation parameter of the liquid in the reaction cup of the microplate.

[0100] If H1 is less than the first preset value, it indicates that the needle set 200 smoothly absorbs liquid and absorbs the preset liquid amount, and if H1 is greater than the first preset value, it indicates that the needle set 200 does not absorb the preset liquid amount, and it is determined that the needle set 200 is blocked. At this time, the staff can replace and maintain the blocked needle set 200 to restore the liquid absorption function of the needle set 200. If H2 is greater than the second preset value, it indicates that the needle set 200 smoothly injects liquid and injects the preset liquid amount of washing liquid into the microplate, and if H2 is less than the second preset value, it indicates that the needle set 200 does not inject the preset liquid amount of washing liquid into the microplate, and it is determined that the needle set 200 is blocked. At this time, the staff can replace and maintain the blocked needle set 200 to restore the liquid injection function of the needle set 200. The first preset value and the second preset value can be set to a numerical value or a numerical range.

[0101] In this way, by detecting the blockage of the needle set 200 in real time during the washing of the microplate, when the needle set 200 is blocked, the staff can replace and maintain the needle set 200 in time to eliminate the blockage failure and keep the needle set 200 always normal liquid injection and absorption functions, thereby improving the reliability and washing effect of the cleaning head in washing the microplate.

[0102] The needle set 200 includes the liquid injection needle 210 and the liquid absorption needle 220. The liquid injection needle 210 is used to perform the liquid injection action, and the liquid absorption needle 220 is used to perform the liquid absorption action. The liquid absorption end 221 of the liquid absorption needle 220 is lower than the liquid injection end 211 of the liquid injection needle 210. Both the liquid absorption needle 220 and the liquid injection needle 210 have a probability of being blocked, which affects the liquid absorption and injection functions of the needle set 200. Therefore, the liquid injection needle 210 and the liquid absorption needle 220 need to be detected for blockage. In an embodiment, the detection module 310 detects the liquid level representation parameter of the microplate by using the liquid absorption needle 220, and realizes the blockage detection of the liquid injection needle 210 and the liquid absorption needle 220. In this case, the blockage detection method includes the following steps: after the liquid absorption needle 220 completes the liquid absorption action, the detection module 310 measures the liquid level representation parameter H1 by using the liquid absorption needle 220; and after the liquid injection needle 210 completes the liquid injection action, the detection module 310 measures the liquid level representation parameter H2 by using the liquid absorption needle 220.

[0103] It should be noted that the detection module 310 completes the blockage detection of the liquid absorption needle 220 and the liquid injection needle 210 by using the liquid absorption needle 220. On the one hand, the liquid injection needle 210 does not need to be connected with the detection module 310, which can simplify the structure of the cleaning head. On the other hand, the liquid absorption needle 220 and the liquid injection needle 210 in each needle set 200 can change positions synchronously, and the relative positions of the two are kept constant. The liquid absorption end 221 is always lower than the liquid injection end 211, and the liquid absorption end 221 contacts the liquid in the reaction cup before the liquid injection end 211. It is more convenient to detect the liquid level representation parameter by using the liquid absorption needle 220. Moreover, the setting mode of the needle set 200 meets the washing needs of the cleaning head for the microplate, and there is no need to modify the structure of the needle set 200 and the microplate, thereby reducing the design complexity and production cost.

[0104] In an embodiment, the liquid level representation parameter H1 and the liquid level representation parameter H2 are set as capacitance values, and the detection module 310 detects the needle blockage by detecting the capacitance values of the liquid suction needle 220. Specifically, the needle blockage detection method includes: before the liquid suction needle 220 performs a liquid suction action, the detection module 310 obtains a capacitance value C0 through the liquid suction needle 220; after the liquid suction needle 220 completes the liquid suction action, the detection module 310 obtains a capacitance value C1 through the liquid suction needle 220, if the result of C1-C0 is less than a first preset difference value, it indicates that the liquid suction of the liquid suction needle 220 is smooth, and the preset liquid amount is sucked, if the result of C1-C0 is greater than the first preset difference value, it indicates that the liquid suction of the liquid suction needle 220 is blocked, and the preset liquid amount is not sucked, then it is determined that the liquid suction needle 220 is blocked; after the liquid injection needle 210 completes the liquid injection action, the detection module 310 obtains a capacitance value C2 through the liquid suction needle 220, if the result of C2-C1 is greater than a second preset difference value, it indicates that the liquid injection of the liquid injection needle 210 is smooth, and the preset liquid amount can be injected into the microplate, if the result of C2-C1 is less than the second preset difference value, it indicates that the liquid injection of the liquid injection needle 210 is blocked, and the preset liquid amount is not injected into the microplate, then it is determined that the liquid injection needle 210 is blocked. The first preset difference value and the second preset difference value can be set as a numerical value, or a numerical range.

[0105] To remind the staff to remove the needle blockage fault, in an embodiment, the needle blockage detection method further includes: after determining that the needle group 200 is blocked, the cleaning head alarms to alert the staff to troubleshoot the needle blockage fault; specifically, when it is determined that the liquid suction needle 220 is blocked and / or the liquid injection needle 210 is blocked, the cleaning head alarms, and the staff handles the needle blockage fault according to the alarm information. Further, when the cleaning head is in an alarm state, in addition to alerting the staff, it can also display fault information to help the staff quickly eliminate the needle blockage fault, and the fault information includes the position of the blocked liquid injection needle 210 or liquid suction needle 220, the degree of blockage, etc.

[0106] In addition, to avoid false judgment of needle blockage and thus affect the washing efficiency of the microplate, in an embodiment of the present application, after determining the needle blockage, a recheck measure is provided to reduce the probability of false judgment; specifically, the needle blockage detection method further includes: after determining that the liquid suction needle 220 is blocked, the cleaning head performs a recheck action. The recheck action includes:

[0107] The liquid suction needle 220 performs n times of liquid suction actions, and after each liquid suction action is completed, the detection module 310 obtains a capacitance value C1 through the liquid suction needle 220, where n is a positive integer. If, during the n times of liquid suction actions of the liquid suction needle 220, the detection module 310 obtains a capacitance value C1 in a certain detection, and the result of C1-C0 is less than the first preset difference value, the clogging determination result of the liquid suction needle 220 is eliminated, the rechecking action is ended, and the detection module 310 at least performs n times of actions of obtaining a capacitance value C1 through the liquid suction needle 220 in this process. If, after the liquid suction needle 220 completes the n times of liquid suction actions, any capacitance value C1 detected by the detection module 310 has a result of C1-C0 greater than the first preset difference value, the clogging determination result of the liquid suction needle 220 is maintained, the rechecking action is ended, and the cleaning head alarms. The detection module 310 performs n times of actions of obtaining a capacitance value C1 through the liquid suction needle 220 in this process. n is not limited to 1, 2, 3, etc.

[0108] Further, in the clogging rechecking step of the liquid suction needle 220, if the clogging determination result of the liquid suction needle 220 is eliminated, the cleaning head can continue to perform the washing step on the microplate, which can specifically be that the liquid suction needle 220 changes position, the liquid injection needle 210 performs a liquid injection action, etc.

[0109] In another embodiment, the liquid suction needle 220 continuously maintains a liquid suction state, that is, the liquid suction needle 220 continuously performs a liquid suction action. In this case, the rechecking action includes that the detection module 310 at least performs n times of actions of obtaining a capacitance value C1 through the liquid suction needle 220. During the n times of actions of obtaining a capacitance value C1 through the liquid suction needle 220 performed by the detection module 310, if the detection module 310 obtains a capacitance value C1 in a certain detection, and the result of C1-C0 is less than the first preset difference value, the clogging determination result of the liquid suction needle 220 is eliminated, the rechecking action is ended; if the detection module 310 completes the n times of actions of obtaining a capacitance value C1 through the liquid suction needle 220, any capacitance value C1 detected by the detection module 310 has a result of C1-C0 greater than the first preset difference value, the clogging determination result of the liquid suction needle 220 is maintained, the rechecking action is ended, and the cleaning head alarms. n is not limited to 1, 2, 3, etc.

[0110] It should be noted that, for the case that the liquid suction needle 220 continuously maintains a liquid suction state, when the cleaning head performs the rechecking action, the detection module 310 performs the actions of obtaining capacitance values C1 adjacent to each other at a preset time interval. For example, when the liquid suction needle 220 is determined to be clogged, after a time t elapses, the detection module 310 performs an action of obtaining a capacitance value C1 through the liquid suction needle 220; if the result of C1-C0 is greater than the first preset difference value, after a time t elapses, the detection module 310 performs the next action of obtaining a capacitance value C1 through the liquid suction needle 220.

[0111] Further, for the case that the liquid suction needle 220 continuously keeps the liquid suction state, in order to avoid that the liquid injection needle 210 injects liquid to the reaction cup of the microplate, the liquid suction needle 220 sucks the liquid in the reaction cup, and affects the washing of the microplate, the needle plug detection method of the present application further comprises: when the washing head washes the microplate, the needle group 200 performs a position transformation action, so that the liquid suction needle 220 avoids the reaction cup of the microplate when the liquid injection needle 210 performs the liquid injection action. Specifically, the position transformation action comprises lifting the needle group 200, and the liquid suction needle 220 and the liquid injection needle 210 in the needle group 200 are synchronously lifted or lowered, the liquid suction end 221 of the liquid suction needle 220 is lower than the liquid injection end 211 of the liquid injection needle 210, when the needle group 200 is lowered, the liquid suction end 221 first contacts the liquid in the reaction cup and sucks the liquid in the reaction cup, after the liquid suction needle 220 completes the liquid suction action and rises, the liquid injection needle 210 starts to perform the liquid injection action, the liquid injection needle 210 injects liquid to the reaction cup in a suspended manner (the lowest point of the liquid injection end 211 is higher than the highest point of the reaction cup), at this time the liquid suction end 221 has exited the reaction cup, and the liquid injection needle 210 can avoid injecting liquid to the reaction cup of the microplate while the liquid suction needle 220 sucks the liquid in the reaction cup.

[0112] In a specific embodiment, the needle group 200 has an initial height, when the needle group 200 is at the initial height, the needle group 200 does not start to perform the liquid suction action and the liquid injection action, at this time, the detection module 310 obtains the capacitance value C0 through the liquid suction needle 220. When the needle group 200 is lowered to the first preset height, the liquid suction needle 220 starts to perform the liquid suction action and sucks the liquid in the reaction cup of the microplate; after the liquid suction needle 220 completes the liquid suction action, the detection module 310 performs the needle blocking detection action on the liquid suction needle 220, that is, obtains the capacitance value C1 through the liquid suction needle 220, if the result of C1-C0 is greater than the first preset difference, it is determined that the liquid suction needle 220 is blocked, if the result of C1-C0 is less than the first preset difference, it is determined that the liquid suction needle 220 is not blocked. If the detection result is that the liquid suction needle 220 is blocked, the cleaning head alarms, or the cleaning head performs the rechecking action; if the detection result is that the liquid suction needle 220 is not blocked, the needle group 200 is raised to the second preset height, the second preset height is higher than the first preset height, so that the liquid suction end 221 exits the reaction cup of the microplate, the liquid injection needle 210 starts to perform the liquid injection action and injects liquid into the reaction cup of the microhole; after the liquid injection needle 210 completes the liquid injection action, the detection module 310 performs the needle blocking detection action on the liquid injection needle 210, that is, obtains the capacitance value C2 through the liquid suction needle 220, if the result of C2-C1 is less than the second preset difference, it is determined that the liquid injection needle 210 is blocked, if the result of C2-C1 is greater than the second preset difference, it is determined that the liquid injection needle 210 is not blocked. If the detection result is that the liquid injection needle 210 is blocked, the cleaning head alarms; if the detection result is that the liquid injection needle 210 is not blocked, the needle group 200 continues to perform the liquid suction action at the first preset height; or the needle group 200 performs the position conversion action, the needle group 200 moves to other areas of the microplate through translation, and then performs the liquid suction action and the liquid injection action on other reaction cups; or the cleaning head ends the washing of the microplate.

[0113] It should be noted that the "liquid suction action" in the present application refers to the process of the liquid suction needle 220 sucking the liquid in the reaction cup of the microplate when the needle group 200 is at the first preset height, when the liquid suction needle 220 is not at the first preset height, it is considered that the liquid suction needle 220 does not start to perform the liquid suction action or the liquid suction action has been completed. Similarly, the "liquid injection action" in the present application refers to the process of the liquid injection needle 210 injecting liquid into the reaction cup of the microplate when the needle group 200 is at the second preset height, when the liquid injection needle 210 is at the second preset height, the liquid injection needle 210 does not inject liquid, it is considered that the liquid injection needle 210 does not start to perform the liquid injection action or the liquid injection action has been completed.

[0114] In addition, when the injection needle 210 performs the injection action, the needle group 200 is at the second preset height, when the injection needle 210 completes the injection action and the detection module 310 starts to perform the needle blocking detection action on the injection needle 210, the injection needle 210 stops injection, and the needle group 200 is lowered from the second preset height to the detection height, at this time, the suction end 221 of the suction needle 220 enters the reaction cup of the microplate again, so that the suction end 221 can be in contact with the liquid in the reaction cup, and the detection module 310 can obtain the capacitance value C2 through the suction needle 220; the detection height can be the same as the first preset height, or higher than the first preset height. When the detection result is that the injection needle 210 is not blocked, and before the needle group 200 continues to perform the suction action, the needle group 200 is switched to the first preset height, so that the suction needle 220 performs the suction action at the first preset height.

[0115] The "position conversion action" in the application includes: the lifting action of the needle group 200 before and after the suction action and the injection action, and the moving action of the needle group 200 after completing the washing of a certain area of the microplate. By setting the moving action of the needle group 200, the cleaning head can wash different areas of the microplate; by setting the lifting action of the needle group 200, the position switching of the needle group 200 at the first preset height, the second preset height and the detection height is realized, which plays a role in avoiding the reaction cup during the injection of the suction needle 220, and the detection module 310 uses the suction needle 220 to detect the blocking of the suction needle 220 and the injection needle 210.

[0116] In the description of the present application, the description of the terms "one embodiment", "some embodiments", "exemplary embodiment", "example", "specific example", or "some examples" means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In the present application, the exemplary description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0117] The embodiments of the application are described in detail above in combination with the drawings, but the application is not limited to the above embodiments, and various changes can be made within the knowledge of those skilled in the art without departing from the purpose of the application.

Claims

1. A cleaning head for a plate washing machine, characterized in that, include: The main body of the cleaning head has a liquid suction channel and a liquid injection channel inside; The needle assembly connects the liquid aspiration channel and the liquid injection channel, and is capable of injecting and aspirating liquid into the microporous reaction plate to wash the microporous reaction plate; The detection component includes a detection module, which is installed on the main body of the cleaning head and electrically connected to the needle assembly. The detection module is used to detect the liquid level characterization parameters of the liquid in the microporous reaction plate through the needle assembly when the needle assembly washes the microporous reaction plate.

2. The cleaning head for a plate washing machine according to claim 1, characterized in that, The needle assembly includes an injection needle and an aspiration needle. The aspiration needle is connected to the aspiration channel, and the injection needle is connected to the injection channel. The aspiration needle includes an aspiration end, which is used to aspirate liquid from the microporous reaction plate. The injection needle includes an injection end, which is used to inject liquid into the microporous reaction plate. The aspiration end is lower than the injection end. The aspiration needle is electrically connected to the detection module, while the injection needle is isolated from the detection module. The detection module can detect the liquid level characterization parameters of the liquid inside the micropore reaction through the aspiration needle.

3. The cleaning head for a plate washing machine according to claim 2, characterized in that, The injection channel and the aspiration channel are located on the same side of the detection module; Alternatively, the injection channel and the suction channel are located on opposite sides of the detection module.

4. The cleaning head for a plate washing machine according to claim 2, characterized in that, The injection channel and the aspiration channel are located on the same side of the detection module. The detection module has a first through hole through which the aspiration needle passes, and the aspiration needle is electrically connected to the inner wall of the first through hole; wherein, The detection module also has a second through hole through which the injection needle passes, and there is a gap between the injection needle and the inner wall of the second through hole; or, the detection module is located on the side of the injection needle, and there is a gap between the detection module and the injection needle.

5. The washing head for a plate washing machine according to any one of claims 2 to 4, characterized in that, The cleaning head body has an internal mounting cavity, a first mounting channel and a second mounting channel. The two ends of the first mounting channel are respectively connected to the mounting cavity and the liquid suction channel, and the two ends of the second mounting channel are respectively connected to the mounting cavity and the liquid injection channel. Part of the aspiration needle is inserted into the first mounting cavity, part of the injection needle is inserted into the second mounting cavity, and the detection module is accommodated in the mounting cavity.

6. The washing head for a plate washing machine according to any one of claims 2 to 4, characterized in that, The plate washing machine cleaning head includes multiple sets of needle groups, and each needle group includes an injection needle and a suction needle. The liquid aspiration channel includes a main channel, multiple branch channels, and multiple aspiration branches. The branch channels are arranged at intervals along the extension direction of the main channel. One end of each branch channel is connected to the main channel, and the other end of each branch channel is connected to at least one aspiration branch. The end of each aspiration branch forms a suction port, and each suction port is connected to a suction needle. The suction ports are arranged along a first direction and / or a second direction. The first direction is parallel to the extension direction of the main channel, and the first direction intersects with the second direction.

7. The cleaning head for a plate washing machine according to claim 1, characterized in that, The plate washing machine cleaning head has a first working state, a second working state, and a third working state. When the plate washing machine cleaning head is in the first working state, the needle group performs one of the following actions: liquid aspiration, liquid injection, and position switching. When the plate washing machine cleaning head is in the second working state, the detection module performs a re-inspection action. When the plate washing machine cleaning head is in the third working state, the plate washing machine cleaning head alarms. The detection component also includes a control module, which is communicatively connected to the detection module. The control module is configured to switch the plate washing head between the first working state, the second working state, and the third working state based on the detection result of the detection module.

8. A method for detecting pin blockage in a plate washing machine, characterized in that, The plate washer cleaning head according to any one of claims 1-7 is used for needle blockage detection, including: During the washing of the microporous reaction plate, the needle assembly performs liquid aspiration and liquid injection actions on the microporous reaction plate; The detection module detects the liquid level characterization parameter H1 of the liquid in the microporous reaction plate when the needle group completes the liquid aspiration action, and the liquid level characterization parameter H2 of the liquid in the microporous reaction plate when the needle group completes the liquid injection action. When H1 is greater than the first preset value, the needle group is determined to be blocked; when H2 is less than the second preset value, the needle group is determined to be blocked.

9. The method for detecting pin blockage in a plate washing machine according to claim 8, characterized in that, The needle assembly includes an injection needle and an aspiration needle. The injection needle is used to perform the injection action, and the aspiration needle is used to perform the aspiration action. The aspiration needle is electrically connected to the detection module, while the injection needle is isolated from the detection module. The plate washer needle blockage detection method includes: After the aspiration needle completes the aspiration action, the detection module measures the liquid level characterization parameter H1 through the aspiration needle; after the injection needle completes the injection action, the detection module measures the liquid level characterization parameter H2 through the aspiration needle.

10. The method for detecting pin blockage in a plate washing machine according to claim 9, characterized in that, The liquid level characterization parameter H1 and the liquid level characterization parameter H2 are set as the capacitance values ​​of the aspiration needle detected by the detection module; The method for detecting pin blockage in the plate washing machine includes: Before the aspiration needle performs the aspiration action, the detection module obtains the capacitance value C0 through the aspiration needle; After the aspiration needle completes the aspiration action, the detection module obtains the capacitance value C1 through the aspiration needle. If the result of C1-C0 is greater than the first preset difference, it is determined that the aspiration needle is blocked. After the injection needle completes the injection action, the detection module obtains the capacitance value C2 through the aspiration needle. If the result of C2-C1 is less than the second preset difference, it is determined that the injection needle is blocked.

11. The method for detecting pin blockage in a plate washing machine according to claim 10, characterized in that, The method for detecting pin blockage in the plate washing machine includes: After determining that the suction needle is blocked, the detection module performs the action of obtaining the capacitance value C1 through the suction needle at least n times; If the result of C1-C0 is greater than the first preset difference, the cleaning head of the plate washing machine will alarm. If the result of C1-C0 is less than the first preset difference, the needle blockage determination result of the aspiration needle is eliminated, or the injection needle performs the injection action; and / or, The plate washer cleaning head alarms after it is determined that the injection needle is blocked.

12. The method for detecting pin blockage in a plate washing machine according to claim 10 or 11, characterized in that, The method for detecting pin blockage in the plate washing machine includes: During the washing of the microporous reaction plate, the aspiration needle and the injection needle rise or fall synchronously, and the aspiration needle continuously maintains the aspiration state; The needle assembly has an initial height. When the needle assembly is at the initial height, the detection module obtains the capacitance value C0 through the aspiration needle. When the needle assembly descends to the first preset height, the aspiration needle performs the aspiration action and draws liquid from the microporous reaction plate; after the aspiration needle completes the aspiration action, the detection module performs a needle blockage detection action on the aspiration needle and obtains the capacitance value C1. If the detection result indicates that the aspiration needle is not blocked, the needle assembly rises to the second preset height, the injection needle begins to perform the injection action, and injects liquid into the microporous reaction plate; after the injection needle completes the injection action, the detection module performs a blockage detection action on the injection needle and obtains the capacitance value C2. If the blockage result indicates that the injection needle is not blocked.

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