Method for identifying lodging resistance of sugarcane variety

Abstract

The invention discloses a sugarcane variety lodging resistance identification method which comprises the following steps: collecting stem base diameter data, stem middle diameter data, stem height data, stem hardness data, root system development degree data and leaf size and weight data; and establishing a lodging resistance evaluation model based on multiple linear regression analysis according to the stem base diameter data, the stem middle diameter data, the stem height data, the stem hardness data, the root system development degree data and the leaf size and weight data. According to the method, a plurality of key indexes influencing the lodging-resistant capability of the sugarcane plants are comprehensively considered, the key indexes comprise the morphological index, the mechanical index, the root system index and the like, and compared with a traditional identification method with a single index or few indexes, the lodging-resistant characteristics of the sugarcane plants can be more comprehensively reflected. By constructing the lodging-resistant capability evaluation model, the lodging-resistant capability evaluation result can be quickly obtained only by substituting the measured data into the model, so that the identification efficiency is greatly improved.

Description

Technical Field

[0001] This invention relates to the field of agricultural planting technology, and in particular to a method for identifying lodging resistance in sugarcane varieties. Background Technology

[0002] Sugarcane, as one of the most important sugar and cash crops in southern my country, relies heavily on mechanization throughout its production process to determine its competitiveness. However, frequent lodging has become a major bottleneck limiting the efficiency of sugarcane combine harvesters and leading to losses in both yield and sugar content. Therefore, establishing a precise and repeatable system for identifying the lodging resistance of sugarcane varieties is not only a prerequisite for breeding highly lodging-resistant varieties but also a crucial common technological requirement for improving sugarcane yield and sugar quality, and promoting the green, efficient, and sustainable development of the industry. Currently, traditional methods for identifying sugarcane lodging resistance have many shortcomings. For example, relying solely on measuring the lodging angle (such as the method in GB / T5262-2008, which uses a protractor to measure the lodging angle and classifies the degree of lodging of sugarcane based on the lodging angle: lodging angle between 0-30° is considered no lodging, lodging angle between 30-60° is considered moderate lodging, and lodging angle greater than 60° is considered severe lodging) is problematic in practice. Due to significant individual differences within the same variety, it requires extensive sampling, making the measurement process cumbersome and complex, and is greatly affected by environmental factors, resulting in inaccurate measurement results. Similarly, using only the ratio of the base stem diameter to the middle stem diameter (LRC value) to assess lodging resistance (when LRC > 0.98, the sugarcane has strong lodging resistance; when 0.95 < LRC ≤ 0.98, the sugarcane has moderate lodging resistance; when LRC ≤ 0.95, the sugarcane has weak lodging resistance) provides a single evaluation indicator and cannot comprehensively and accurately reflect the lodging resistance of sugarcane varieties in different growing environments and stages. Summary of the Invention

[0003] The main objective of this invention is to provide a method for identifying the lodging resistance of sugarcane varieties, aiming to solve the problem that existing methods for identifying sugarcane lodging resistance have a single evaluation index and cannot comprehensively and accurately reflect the lodging resistance of sugarcane varieties.

[0004] To achieve the above objectives, this invention proposes a method for identifying the lodging resistance of sugarcane varieties, comprising the following steps: collecting data on stem base diameter, stem mid-section diameter, stem height, stem hardness, root system development, and leaf size and weight. The stem base and mid-section diameter data reflect the stem's supporting capacity; the stem height data reflects the sugarcane plant's center of gravity; the stem hardness data reflects the stem's strength; the root system development data reflects the sugarcane plant's grip on the soil; and the leaf size and weight data reflect the resistance the sugarcane plant experiences in the wind. Based on the stem base diameter, stem mid-section diameter, stem height, stem hardness, root system development, and leaf size and weight data, a lodging resistance evaluation model based on multiple linear regression analysis is established.

[0005] Preferably, the sampling process for the stem base diameter data and the stem middle diameter data is as follows: when the sugarcane has grown to 6-8 months, the stem base diameter is measured at a distance of 2-5cm from the ground, and the stem middle diameter is measured at a distance of 100-160cm from the ground. Each plant is measured 3 times, and the average value is taken.

[0006] Preferably, the sampling process for the stem height data is as follows: using a length measuring tool, the length is measured vertically from the base of the sugarcane plant to the base of the first fully unfolded leaf counted downwards from the top of the sugarcane plant, and the stem height value is recorded.

[0007] Preferably, the stem hardness data is measured using a plant stem strength tester. The stem hardness is measured at the middle of each sugarcane stem, and the measurement is repeated 3 times for each sugarcane, and the average value is taken.

[0008] Preferably, the sampling process for the root system development data is as follows: dig up the complete root system of the sugarcane plant, wash off the soil on the root system, take root system images using an image acquisition device, analyze the root system images, and obtain the total root length, root surface area, and root volume parameters.

[0009] Preferably, select the 3rd to 5th fully unfolded leaves from the top of the sugarcane plant, measure the length and width of the leaves with a ruler, calculate the leaf area, cut off the measured leaves, weigh them using an electronic balance with an accuracy of 0.01g, record the leaf weight, measure 3 leaves from each plant, and take the average value.

[0010] Preferably, the lodging resistance evaluation model is established as follows: , where Y represents the lodging resistance evaluation value, a1, a2, a3, a4, a5, a6, a7 are regression coefficients, b is a constant term, X1 is the stem base diameter, X2 is the stem middle diameter, X3 is the stem height, X4 is the stem hardness, X5 is the total root length, X6 is the leaf area, and X7 is the leaf weight.

[0011] Preferably, the lodging resistance evaluation value Y is calculated based on the lodging resistance evaluation model, and the lodging resistance of sugarcane plants is graded based on the calculated lodging resistance evaluation value Y.

[0012] Preferably, the lodging resistance level is established as three levels: when Y≥80, the lodging resistance is determined to be strong; when 60≤Y<80, the lodging resistance is determined to be medium; and when Y<60, the lodging resistance is determined to be weak.

[0013] Compared with the prior art, the beneficial effects of the present invention are as follows: 1. The method for identifying lodging resistance of sugarcane varieties described in this invention comprehensively considers multiple key indicators affecting the lodging resistance of sugarcane varieties, including morphological indicators, mechanical indicators, and root system indicators. Compared with traditional identification methods that use only one or a few indicators, this method can more comprehensively reflect the lodging resistance characteristics of sugarcane varieties.

[0014] 2. The lodging resistance identification method for sugarcane varieties described in this invention, through scientific measurement methods and a lodging resistance evaluation model constructed based on a large amount of sample data, can accurately calculate the lodging resistance evaluation value of sugarcane varieties and accurately classify them, reducing the errors caused by human and environmental factors in traditional methods and improving the accuracy and reliability of the identification results.

[0015] 3. The measurement methods and equipment used in this invention are commonly used in the agricultural field, are relatively simple to operate, easy to master, and do not require complex professional skills or expensive equipment, which is conducive to their widespread application in actual production and scientific research. Furthermore, by constructing a lodging resistance evaluation model, the lodging resistance evaluation results can be quickly obtained simply by substituting the measured data into the model, greatly improving the evaluation efficiency. Detailed Implementation

[0016] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0017] It should be understood that, when used in this specification and the appended claims, the terms "comprising" and "including" indicate the presence of the described features, integrals, steps, operations, elements and / or components, but do not exclude the presence or addition of one or more other features, integrals, steps, operations, elements, components and / or collections thereof.

[0018] It should also be understood that the terminology used in this specification is for the purpose of describing particular embodiments only and is not intended to limit the invention. As used in this specification and the appended claims, the singular forms “a,” “an,” and “the” are intended to include the plural forms unless the context clearly indicates otherwise.

[0019] It should also be further understood that the term "and / or" as used in this specification and the appended claims refers to any combination of one or more of the associated listed items and all possible combinations, and includes such combinations.

[0020] Each sugarcane variety possesses unique characteristics, including stem diameter, stem height, stem hardness, root system development, leaf size and length, etc. These traits reflect the variety's lodging resistance. Therefore, this invention provides a method for identifying the lodging resistance of sugarcane varieties, comprising the following steps: First, data on stem base diameter, stem mid-section diameter, stem height, stem stiffness, root system development, and leaf size and weight were collected. Stem base and mid-section diameter data reflect the stem's supporting capacity; stem height reflects the sugarcane plant's center of gravity; stem stiffness reflects the stem's mechanical strength; root system development reflects the sugarcane plant's grip on the soil; and leaf size and weight reflect the resistance the sugarcane plant experiences in the wind. Based on these data, a lodging resistance evaluation model using multiple linear regression analysis was established. The lodging resistance evaluation value Y was calculated using this model, and the calculated Y value was used to classify the sugarcane plant's lodging resistance.

[0021] (1) Preparation of experimental materials The two sugarcane varieties, Guitang 42 (resistant to lodging) and Guitang 52 (prone to lodging), were selected. Fifty plants of each variety were planted, for a total of 100 plants, in an experimental field with the same soil conditions, climate, and cultivation management. (2) Index measurement Measurement of stem base diameter and stem mid-section diameter: Using a vernier caliper with an accuracy of 0.01 mm, when the sugarcane has grown to 6-8 months, the stem base diameter was measured at a point 2-5 cm above the ground, and the stem mid-section diameter was measured at a point 100-160 cm above the ground. Each plant was measured 3 times, and the average value was taken. The stem base diameter and stem mid-section diameter of 20 plants of each variety were measured and the data were recorded. Stem height measurement: While measuring the diameter at the base and middle of the stem, use a ruler or measuring rod to measure the stem height of the same batch of plants. Measure vertically from the base of the sugarcane plant to the base of the first fully unfolded leaf, accurate to 1 cm, and record the data. Stalk hardness measurement: A plant stalk strength tester was used to measure the stalk hardness in the middle of each sugarcane stalk. The probe of the tester was pressed vertically into the middle of the sugarcane stalk, and pressure was slowly applied until the stalk showed obvious deformation or broke. The stalk hardness value displayed on the tester was read, and the unit was N. Each plant was measured 3 times, the average value was taken, and the data was recorded. Measurement of Root System Development: When sugarcane reaches 9 months of growth, the root systems of 10 plants of each variety are excavated, and images of the root systems are taken using image acquisition equipment (such as a digital camera). Professional root system analysis software (such as the WinRHIZO root system analysis system) is used to analyze the root system images, obtaining parameters such as total root length, root surface area, and root volume to comprehensively assess the root system development. The total root length is used as the primary evaluation indicator. Leaf size and weight data measurement: While measuring the root system, select the 3rd to 5th fully unfolded leaves from the top of 10 plants of each variety, measure the length and width of the leaves with a ruler, calculate the leaf area, cut off the measured leaves, dry them and weigh them using an electronic balance with an accuracy of 0.01g, record the leaf weight, measure 3 leaves from each plant, take the average value and record the data. Based on all the above data, a lodging resistance evaluation model was constructed: By measuring the above indicators of a large number of sugarcane plants of different varieties and combining this with statistical analysis of actual field lodging conditions, a lodging resistance evaluation model based on multiple linear regression analysis was established. Let the lodging resistance evaluation value be Y, the stem base diameter be X1 (mm), the stem mid-section diameter be X2 (mm), the stem height be X3 (cm), the stem hardness be X4 (N), the total root length be X5 (cm), the leaf area be X6 (cm²), and the leaf weight be X7 (g). Then, the lodging resistance evaluation model is established as: Y = Where a1, a2, a3, a4, a5, a6, and a7 are the regression coefficients for each indicator, and b is the constant term. Statistical analysis was performed on the above 100 sample data to determine the specific values ​​of the regression coefficients and the constant term.

[0022] All data from 100 sugarcane plants were collected and analyzed using statistical analysis software (such as SPSS) to determine the regression coefficients a1, a2, a3, a4, a5, a6, a7, and the constant term b in the lodging resistance evaluation model. The data analysis yielded regression coefficients a1=0.482, a2=0.347, a3=-0.176, a4=0.253, a5=0.121, a6=-0.042, a7=-0.019, and the constant term b=22.531. Therefore, the lodging resistance evaluation model is transformed into: Y=0.482X1+0.347X2-0.176X3+0.253X4+0.121X5-0.042X6-0.019X7+22.531.

[0023] (3) The sampling data for each variety are shown in the table below: Table 1. Data collected on Guitang No. 42 (partial) Table 2. Data collected on Guitang No. 52 (partial) Table 3 Summary of total data for Guitang No. 42 and Guitang No. 52 Lodging resistance grading: Various measurement data of each sugarcane variety are input into the constructed lodging resistance evaluation model to calculate the lodging resistance evaluation value Y. The lodging resistance of the sugarcane plants is then graded based on the calculated evaluation value Y. The lodging resistance grading is constructed into three levels: when Y ≥ 80, the lodging resistance is determined to be strong; when 60 ≤ Y < 80, the lodging resistance is determined to be medium; and when Y < 60, the lodging resistance is determined to be weak. (4) Repeatability test Field Validation: The lodging performance of sugarcane plants of various varieties under natural growth conditions was observed in the experimental field. A random sample of 10% was used, and the lodging resistance grading results obtained using the identification method of this invention, within an allowable error range of ±0.1 mm for stem diameter and ±1.5 N for stem hardness, were compared with the actual growth conditions for verification. After one growing season of observation, it was found that the lodging resistance grading results obtained by the identification method of this invention were highly consistent with the actual lodging situation in the field, further proving the accuracy and reliability of the identification method of this invention.

[0024] In summary, the lodging resistance identification method for sugarcane varieties provided by this invention comprehensively considers multiple key indicators affecting the lodging resistance of sugarcane varieties, including morphological, mechanical, and root system indicators. Compared with traditional identification methods that rely on a single or few indicators, this method can more comprehensively reflect the lodging resistance characteristics of sugarcane varieties. Through scientific measurement methods and a lodging resistance evaluation model constructed based on a large amount of sample data, the lodging resistance evaluation value of sugarcane varieties can be accurately calculated and accurately graded, reducing errors caused by human and environmental factors in traditional methods and improving the accuracy and reliability of the identification results. Furthermore, the method is relatively simple to operate, easy to master, and does not require complex professional techniques or expensive equipment, making it suitable for widespread application in actual production and scientific research. By constructing the lodging resistance evaluation model, the lodging resistance evaluation results can be quickly obtained simply by substituting the measured data into the model, greatly improving the identification efficiency.

[0025] The above description is merely a preferred embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structural transformations made using the contents of the present invention under the inventive concept of the present invention, or direct / indirect applications in other related technical fields, are included within the patent protection scope of the present invention.

Claims

1. A method for identifying lodging resistance in sugarcane varieties, characterized in that, The process includes the following steps: collecting data on stem base diameter, stem mid-section diameter, stem height, stem stiffness, root system development, and leaf size and weight. Stem base and mid-section diameter data reflect the stem's supporting capacity; stem height data reflects the sugarcane plant's center of gravity; stem stiffness data reflects stem strength; root system development data reflects the sugarcane plant's grip on the soil; and leaf size and weight data reflect the resistance the sugarcane plant experiences in the wind. Based on these data, a lodging resistance evaluation model based on multiple linear regression analysis is established.

2. The method for identifying lodging resistance of sugarcane varieties according to claim 1, characterized in that, The sampling process for the stem base diameter data and the stem middle diameter data is as follows: when the sugarcane has grown to 6-8 months, the stem base diameter is measured at a distance of 2-5cm from the ground, and the stem middle diameter is measured at a distance of 100-160cm from the ground. Each plant is measured 3 times, and the average value is taken.

3. The method for identifying lodging resistance of sugarcane varieties according to claim 1, characterized in that, The sampling process for the stem height data is as follows: using a length measuring tool, the length is measured vertically from the base of the sugarcane plant to the base of the first fully unfolded leaf counted downwards from the top of the sugarcane plant, and the stem height value is recorded.

4. The method for identifying lodging resistance of sugarcane varieties according to claim 1, characterized in that, The stem hardness data was measured using a plant stem strength tester. The stem hardness was measured at the middle of each sugarcane stem, and each sugarcane was measured three times, and the average value was taken.

5. The method for identifying lodging resistance of sugarcane varieties according to claim 1, characterized in that, The sampling process for the root system development data is as follows: dig up the complete root system of the sugarcane plant, wash off the soil on the root system, take root system images using image acquisition equipment, analyze the root system images, and obtain the total root length, root surface area, and root volume parameters.

6. The method for identifying lodging resistance of sugarcane varieties according to claim 1, characterized in that, Select the 3rd to 5th fully unfolded leaves from the top of the sugarcane plant. Use a ruler to measure the length and width of the leaves and calculate the leaf area. Cut off the measured leaves and weigh them using an electronic balance with an accuracy of 0.01g. Record the weight of the leaves. Measure 3 leaves from each plant and take the average value.

7. The method for identifying lodging resistance of sugarcane varieties according to claim 1, characterized in that, The lodging resistance evaluation model is established as follows: , where Y represents the lodging resistance evaluation value, a1, a2, a3, a4, a5, a6, a7 are regression coefficients, b is a constant term, X1 is the stem base diameter, X2 is the stem middle diameter, X3 is the stem height, X4 is the stem hardness, X5 is the total root length, X6 is the leaf area, and X7 is the leaf weight.

8. The method for identifying lodging resistance of sugarcane varieties according to claim 1, characterized in that, Based on the lodging resistance evaluation value Y calculated by the lodging resistance evaluation model, the lodging resistance of sugarcane varieties is graded.

9. The method for identifying lodging resistance of sugarcane varieties according to claim 1, characterized in that, The lodging resistance level is established in three levels: when Y≥80, the lodging resistance is determined to be strong; when 60≤Y<80, the lodging resistance is determined to be medium; and when Y<60, the lodging resistance is determined to be weak.

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