A medfly attractant

Abstract

The present invention relates to a kind of medfly attractant.The present invention finds that geranyl acetate, D-limonene and trans-2-octene-1-al can all attract medfly adults, in particular, geranyl acetate and D-limonene can synergistically attract medfly adults, more particularly, geranyl acetate, D-limonene and trans-2-octene-1-al can further synergistically attract medfly adults.

Description

Technical Field

[0001] This invention relates to the field of attractants, and in particular to a wolfberry fruit fly attractant. Background Technology

[0002] Goji berry fruit fly ( Neoceratitis asiatica The goji berry fruit fly (Lycium barbarum) is a fruit-boring pest that damages the medicinal herb wolfberry. Because the larvae feed inside the fruit throughout their entire life cycle, they are difficult to control, severely impacting the quality and yield of goji berries. However, the adult male and female goji berry fruit flies, after emerging from the soil, are exposed to the environment for extended periods and can be considered a key target for control in production.

[0003] Most existing control methods are chemical control, but chemical pesticides are prone to causing resistance, and chemical pesticides will bring a series of problems such as environmental pollution and pesticide residues.

[0004] Therefore, screening for volatile compounds that attract wolfberry fruit flies is beneficial for achieving green pest control. Summary of the Invention

[0005] One aspect of the present invention provides the use of at least one of geraniol acetate, D-limonene, and trans-2-octen-1-ol in attracting adult fruit flies.

[0006] The second invention provides a composition comprising geraniol acetate and D-limonene.

[0007] In one specific embodiment, the mass ratio of geraniol acetate to D-limonene is 1:1.

[0008] In one embodiment, the composition further includes trans-2-octen-1-ol.

[0009] In one specific embodiment, the mass ratio of geraniol acetate, D-limonene, and trans-2-octen-1-ol is 1:1:1.

[0010] In one embodiment, the composition further includes a pesticide-acceptable carrier.

[0011] In one specific embodiment, the pesticide-acceptable carrier is a solvent, and the solvent is an alkane.

[0012] In one specific embodiment, the pesticide-acceptable carrier is a sustained-release carrier.

[0013] In one specific embodiment, the sustained-release carrier is at least one of a rubber-tip carrier (e.g., a desulfurized rubber stopper carrier), a cotton core carrier, and a microtubule carrier.

[0014] The third invention provides the application of the combination according to any one of the second invention in attracting adult wolfberry fruit flies.

[0015] The beneficial effects of the present invention are as follows: The present invention has found that geraniol acetate, D-limonene, and trans-2-octen-1-ol can all attract adult fruit flies of the wolfberry plant. In particular, geraniol acetate and D-limonene can play a synergistic role in attracting adult fruit flies of the wolfberry plant. More specifically, geraniol acetate, D-limonene, and trans-2-octen-1-ol can play an even more synergistic role in attracting adult fruit flies of the wolfberry plant. Attached Figure Description

[0016] Figure 1 The behavioral responses of adult female and male *Bacillus hygrophila* to geraniol acetate, D-limonene, and trans-2-octen-1-ol were shown.

[0017] Figure 2 The results show the number of wolfberry fruit flies captured in the field on the first day after each lure was suspended.

[0018] Figure 3 The results show the number of wolfberry fruit flies captured in the field on the 7th day after each lure was suspended. Detailed Implementation

[0019] The present invention will be further described in detail below through preferred embodiments, but these embodiments do not constitute a limitation thereof.

[0020] Unless otherwise specified, the reagents and other materials used in the embodiments of this invention can be purchased commercially.

[0021] From June to August 2024, wolfberry fruit fly pupae were collected from the Zhongning wolfberry planting base in Ningxia Hui Autonomous Region (the wolfberry variety was Ningqi No. 5, and the trees were 5 years old). The wolfberry fruit fly pupae were placed in petri dishes (3 cm high and 20 cm in diameter) containing about 3 cm of sandy loam soil. The petri dishes were then placed in a net cage (50 cm long × 50 cm wide × 50 cm high) and reared in an artificial climate chamber (Ningbo Saifu Experimental Instrument Co., Ltd., PRX-450 C).

[0022] The rearing conditions for the wolfberry fruit fly were: photoperiod 14 L:10 D, temperature (25 ± 2) degrees Celsius, and relative humidity 40% ± 10%. After the adults emerged, the unmated female and male adults were placed in separate net cages (50 cm long × 50 cm wide × 50 cm high) and fed with an aqueous solution of yeast extract and sucrose in a mass ratio of 1 g: 3 g dissolved in 10 ml of water.

[0023] Example 1: Indoor Y-type olfactory instrument test

[0024] The behavioral responses of female and male adult fruit flies to geranyl acetate, D-limonene, and trans-2-octen-1-ol standards were tested using a Y-type olfactometer.

[0025] Geranilide acetate solution: Geranilide acetate is dissolved in n-hexane to prepare a geranilide acetate solution with a concentration of 1 μg / μL.

[0026] D-Limonene Solution: Dissolve D-limonene in n-hexane to prepare a D-limonene solution with a concentration of 1 μg / μL.

[0027] trans-2-octen-1-ol solution: Dissolve trans-2-octen-1-ol in n-hexane to prepare a trans-2-octen-1-ol solution with a concentration of 1 μg / μL.

[0028] Take 10 μL of geraniol acetate solution and drop it onto clean filter paper (1 cm × 5 cm). Place the filter paper at the end of one arm of a Y-shaped olfactometer, and place filter paper with an equal amount of n-hexane at the end of the other arm. Use an atmospheric sampler to pump activated carbon-filtered air into the main arm of the Y-shaped olfactometer at an airflow rate of 300 mL / min. Place one male or female wolfberry fly in the main arm of the Y-shaped olfactometer at a time, and observe the behavior of the test insects for 5 minutes. If the wolfberry fly enters a side arm and stays for 1 minute or more beyond 1 / 3 of the side arm's length, it indicates that the test insect has made a clear choice of the odor source connected to that side arm; otherwise, it is recorded as no choice. After every 5 tests, the experimental group (test compound) and the blank control group (n-hexane) are swapped to eliminate the influence of position effect on the test insects. Replace the clean Y-shaped tube after every 10 tests. After the experiment, wash the Y-shaped tube with water and dry it in a 180°C oven for 2 hours. The indoor temperature for the behavioral experiment was 25 ± 1 degrees Celsius, and the humidity was 55% ± 5%. The selection results were then statistically analyzed, and the selection rate was calculated. Forty adult female or male fruit flies were used in each treatment. The results are shown below. Figure 1 .

[0029] The geraniol acetate solution was replaced with D-limonene solution, and all other tests were the same as above to determine the attraction effect of D-limonene on female or male adult fruit flies. The results are shown below. Figure 1 .

[0030] The geraniol acetate solution was replaced with trans-2-octen-1-ol solution, and all other tests were the same as above, to determine the attraction effect of D-limonene on female or male adult fruit flies. The results are shown below. Figure 1 .

[0031] according to Figure 1 The results showed that at a dosage of 10 micrograms, geraniol, D-limonene, and trans-2-octen-1-ol had a strong attraction to both male and female adult fruit flies.

[0032] Example 2: Field core decoy test

[0033] Geraniol acetate, D-limonene, and trans-2-octen-1-ol were each prepared into a 1 μg / μL solution using hexane as a solvent.

[0034] Using n-hexane as a negative control, 100 μL of n-hexane was dropped onto a blank desulfurized rubber stopper carrier measuring 100 mm × 200 mm to create a core, which was designated as CK.

[0035] 100 μL of the 1 μg / μL geranyl acetate solution prepared above was added to a blank desulfurized rubber stopper carrier to prepare the inducer S1.

[0036] 100 μL of the 1 μg / μL D-limonene solution prepared above was added to a blank desulfurized rubber stopper carrier to prepare the inducer S2.

[0037] 100 μL of the 1 μg / μL trans-2-octen-1-ol solution prepared above was added to a blank desulfurized rubber stopper carrier to prepare the inducer S3.

[0038] The 1 μg / µL geranyl acetate solution and the 1 μg / µL D-limonene solution prepared above were mixed at a volume ratio of 1:1. Then, 100 µL of the mixture was added to a blank desulfurized rubber stopper carrier to prepare the inducer S4.

[0039] The 1 μg / µL geranyl acetate solution and the 1 μg / µL D-limonene solution prepared above were mixed at a volume ratio of 1:2. Then, 100 µL of the mixture was added to a blank desulfurized rubber stopper carrier to prepare the inducer S5.

[0040] The 1 μg / µL geranyl acetate solution and the 1 μg / µL D-limonene solution prepared above were mixed at a volume ratio of 2:1. Then, 100 µL of the mixture was added to a blank desulfurized rubber stopper carrier to prepare the inducer S6.

[0041] The 1 μg / µL geranyl acetate solution and the 1 μg / µL trans-2-octen-1-ol solution prepared above were mixed in a 1:1 volume ratio. Then, 100 µL of the mixture was added to a blank desulfurized rubber stopper carrier to prepare the inducer S7.

[0042] The 1 μg / µL geranyl acetate solution and the 1 μg / µL trans-2-octen-1-ol solution prepared above were mixed at a volume ratio of 1:2. Then, 100 µL of the mixture was added to a blank desulfurized rubber stopper carrier to prepare the inducer S8.

[0043] The 1 μg / µL geranyl acetate solution and the 1 μg / µL trans-2-octen-1-ol solution prepared above were mixed at a volume ratio of 2:1. Then, 100 µL of the mixture was added to a blank desulfurized rubber stopper carrier to prepare the inducer S9.

[0044] The 1 μg / µL D-limonene solution and 1 μg / µL trans-2-octen-1-ol solution prepared above were mixed at a volume ratio of 1:1. Then, 100 µL of the mixture was added to a blank desulfurized rubber stopper carrier to prepare the inducer S10.

[0045] The 1 μg / µL D-limonene solution and 1 μg / µL trans-2-octen-1-ol solution prepared above were mixed at a volume ratio of 1:2. Then, 100 µL of the mixture was added to a blank desulfurized rubber stopper carrier to prepare the inducer S11.

[0046] The 1 μg / µL D-limonene solution and 1 μg / µL trans-2-octen-1-ol solution prepared above were mixed at a volume ratio of 2:1. Then, 100 µL of the mixture was added to a blank desulfurized rubber stopper carrier to prepare the inducer S12.

[0047] The 1 μg / µL geranyl acetate solution, 1 μg / µL D-limonene solution and 1 μg / µL trans-2-octen-1-ol solution prepared above were mixed in a volume ratio of 1:1:1. Then, 100 µL of the mixture was added to a blank desulfurized rubber stopper carrier to prepare the inducer S13.

[0048] The 1 μg / µL geranyl acetate solution, 1 μg / µL D-limonene solution and 1 μg / µL trans-2-octen-1-ol solution prepared above were mixed in a volume ratio of 1:1:2. Then, 100 µL of the mixture was added to a blank desulfurized rubber stopper carrier to prepare the inducer S14.

[0049] The 1 μg / µL geranyl acetate solution, 1 μg / µL D-limonene solution and 1 μg / µL trans-2-octen-1-ol solution prepared above were mixed in a volume ratio of 1:1:3. Then, 100 µL of the mixture was added to a blank desulfurized rubber stopper carrier to prepare the inducer S15.

[0050] The experiment was conducted in July 2025 at a wolfberry base in Golmud City, Qinghai Province. The prepared lures were placed in the center of bottle-shaped traps (6 cm in diameter at the bottom and 8 cm in length). The bottle-shaped traps were suspended from wolfberry tree branches, approximately 1 m above the ground, with adjacent traps spaced 10 m apart. Hexane lures were used as a control. Each treatment (each lure) was replicated three times in randomized arrangements. The number of adult wolfberry flies attracted was investigated 1 day and 7 days after setup. The number of wolfberry flies attracted by different treatments was statistically analyzed. The results are shown below. Figure 2 and Figure 3 .

[0051] according to Figure 2 and Figure 3 The results showed that geraniol acetate, D-limonene, and trans-2-octen-1-ol could all attract adult wolfberry fruit flies; combining geraniol acetate and D-limonene in a 1:1 mass ratio (S4) significantly increased the attraction of adult wolfberry fruit flies. P The attraction effect of <0.05% on adult fruit flies of the wolfberry tree was 1.49 times (1 day) and 1.39 times (7 days) of S1, and 1.72 times (1 day) and 1.67 times (7 days) of S2, indicating that the two can have a synergistic effect when combined in a 1:1 mass ratio. Combining geraniol acetate, D-limonene, and trans-2-octen-1-ol in a 1:1:1 mass ratio (S13) can further significantly improve the effect. P The attraction effect of < 0.05 on adult wolfberry fruit flies was 2.76 times (1 day) and 2.82 times (7 days) of S1, 3.18 times (1 day) and 3.39 times (7 days) of S2, 4.77 times (1 day) and 5.91 times (7 days) of S3, and 1.85 times (1 day) and 2.03 times (7 days) of S4. This indicates that the three can further play a synergistic role when combined in a mass ratio of 1:1:1.

Claims

1. A composition for attracting adult fruit flies of the wolfberry tree, comprising geraniol acetate, D-limonene and trans-2-octen-1-ol, wherein the mass ratio of geraniol acetate, D-limonene and trans-2-octen-1-ol is 1:1:

1.

2. The composition according to claim 1, characterized in that, The composition also includes a pesticide-acceptable carrier.

3. The composition according to claim 2, characterized in that, The pesticide-acceptable carrier is a solvent, and the solvent is an alkane.

4. The composition according to claim 2, characterized in that, The pesticide-acceptable carrier is a slow-release carrier.

5. The composition according to claim 4, characterized in that, The sustained-release carrier is at least one of rubber-tip carrier, cotton-core carrier, and microtubule carrier.

6. The use of the composition according to any one of claims 1 to 5 in attracting adult wolfberry fruit flies.

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