Preparation device of naringin complex loaded by octenyl succinic acid modified porous starch ester

Abstract

The present application relates to the technical fields of medical health food, and discloses a preparation device for naringin compound loaded by octenyl succinic acid modified porous starch ester, which comprises a preparation liquid ultrasonic mixing cylinder assembly, a mixing top cover assembly is arranged on the top of the preparation liquid ultrasonic mixing cylinder assembly, and a mixed liquid shaking and stirring assembly is arranged on the mixing top cover assembly; a rocker arm base assembly is rotatably arranged at the bottom of the preparation liquid ultrasonic mixing cylinder assembly; a preparation liquid mixing cylinder assembly is fixedly arranged on both sides of the top of the preparation liquid ultrasonic mixing cylinder assembly; the preparation liquid ultrasonic mixing cylinder assembly comprises a preparation liquid ultrasonic mixing cylinder, a plurality of mixing cylinder bottom arm tables are fixedly arranged on the preparation liquid ultrasonic mixing cylinder through reinforcing rib plates, and a bottom arm table main shaft rod is fixedly arranged on the mixing cylinder bottom arm table; an aeration pipe is fixedly arranged at the bottom of the preparation liquid ultrasonic mixing cylinder, and a liquid pump is fixedly arranged outside the bottom of the preparation liquid ultrasonic mixing cylinder. The present application has the characteristics of sufficient mixing.

Description

Technical Field

[0001] This invention relates to the field of pharmaceutical and health food technology, and in particular to an apparatus for preparing an octenyl succinic acid modified porous starch ester loaded with naringin complex. Background Technology

[0002] Naringin (NA) is an important dihydrocitrus flavonoid compound widely found in Rutaceae plants, possessing various biological activities such as anti-inflammatory, antibacterial, antitumor, and lipid-lowering effects. However, NA has low solubility in water and is easily decomposed in saliva and gastric juice, resulting in low bioavailability. Therefore, protecting and transporting naringin using suitable carriers will improve its bioavailability, which will greatly expand its applications in food, cosmetics, and pharmaceuticals.

[0003] Octenyl succinate modified porous starchester (OSAPS) is a starch-based transporter with both adsorption and amphiphilic properties, giving it both loading and solubilizing capabilities. Furthermore, when the degree of esterification of octenyl succinate is less than 3%, it is a recognized safe food additive. Therefore, OSAPS is a promising transporter; however, how to use OSAPS to load naringin remains a challenge. Summary of the Invention

[0004] In order to solve the technical problems existing in the bioutilization of naringin, the present invention provides a device for preparing a well-mixed porous starch ester loaded with naringin.

[0005] The first technical solution of the present invention: an apparatus for preparing octenyl succinic acid modified porous starch ester supported on naringin complex, comprising a preparation liquid ultrasonic mixing cylinder assembly, a mixing top cover assembly provided on the top of the preparation liquid ultrasonic mixing cylinder assembly, a mixing liquid shaking and stirring assembly provided on the mixing top cover assembly; a rocker arm base assembly rotatably provided on the bottom of the preparation liquid ultrasonic mixing cylinder assembly; preparatory liquid mixing cylinder assemblies fixedly provided on both sides of the top of the preparation liquid ultrasonic mixing cylinder assembly; the preparation liquid ultrasonic mixing cylinder assembly includes a preparation liquid ultrasonic mixing cylinder, a plurality of mixing cylinder bottom arm platforms fixedly provided on the preparation liquid ultrasonic mixing cylinder by means of reinforcing ribs, and a bottom arm platform main shaft fixedly provided on the mixing cylinder bottom arm platform; an aeration pipe fixedly provided at the bottom end inside the preparation liquid ultrasonic mixing cylinder, and a liquid pump fixedly provided on the outer side of the bottom of the preparation liquid ultrasonic mixing cylinder. In this invention, two preparatory liquid mixing cylinder assemblies are fixedly installed on both sides of the top of the ultrasonic mixing cylinder assembly of the preparation liquid. The two sets of preparatory liquid mixing cylinder assemblies on the top of the ultrasonic mixing cylinder assembly of the preparation liquid are used as naringin dissolving cylinders and octenyl succinic acid porous starch mixing cylinders, respectively. Naringin and acetone are added to one set of preparatory liquid mixing cylinder assemblies and stirred to prepare solution A. Octenyl succinic acid porous starch and petroleum ether are added to the other set of preparatory liquid mixing cylinder assemblies and stirred to prepare suspension B. Then, solutions A and suspension B from the two sets of preparatory liquid mixing cylinder assemblies are added to the ultrasonic mixing cylinder assembly of the preparation liquid in a specific ratio. At this time, a mixing top cover assembly is installed on the top of the ultrasonic mixing cylinder assembly of the preparation liquid, and a mixing liquid shaking and stirring assembly is installed on the mixing top cover assembly. An aeration pipe is fixedly installed at the bottom of the ultrasonic mixing cylinder for preparing the liquid. This aeration pipe is connected to an external high-pressure air supply tank. High-pressure air supply through the aeration pipe causes the liquid at the bottom of the ultrasonic mixing cylinder to tumble upwards, further ensuring thorough mixing of the solution inside the cylinder. This invention achieves circular shaking of the ultrasonic mixing cylinder assembly and the preparative liquid mixing cylinder assembly on a rocker arm base assembly. This external shaking drive further achieves mixing of the liquids inside the ultrasonic mixing cylinder assembly and the preparative liquid mixing cylinder assembly. Through this external shaking mixing and internal mixing within the cylinder, thorough mixing of the two-stage preparation solutions is achieved. This method ensures thorough and efficient mixing during the preparation of porous starch ester loaded with naringin. The aeration pipe is connected to an external high-pressure air supply tank. High-pressure air supply through the aeration pipe causes the liquid at the bottom of the ultrasonic mixing cylinder to tumble upwards, ensuring thorough mixing of the solution inside the ultrasonic mixing cylinder.

[0006] Preferably, the mixing top cover assembly includes a mixing top cover, inside which a top cover spray pipe is fixedly installed. A top cover frame plate, a vent pipe, and a top cover center ball seat are fixedly installed on the top of the mixing top cover. A top cover drive motor is fixedly installed on the top of the top cover frame plate, and an inclined gripping tube is fixedly installed on the shaft of the top cover drive motor via a support side plate. The top cover spray pipe is fixedly installed inside the mixing top cover and is connected to an external high-pressure liquid delivery pump. This facilitates high-pressure flushing when the device is not in use.

[0007] Preferably, the rocker arm base assembly includes an annular rocker arm base frame, with multiple rocker arm side platforms symmetrically distributed and fixedly arranged along the edge of the annular rocker arm base frame, and frame support legs fixedly arranged at the bottom of the rocker arm side platforms; a first fixed plate is fixedly arranged inside the annular rocker arm base frame via a first support rod, a drive motor is fixedly arranged at the bottom of the first fixed plate, a second fixed plate is fixedly arranged on the shaft of the drive motor, and an annular central gear is fixedly arranged outside the second fixed plate via a second support rod; a rotating main arm shaft is rotatably arranged on the top of the rocker arm side platform via a bearing seat, a support arm is fixedly arranged on the top of the rotating main arm shaft, and the distal end of the support arm is rotatably connected to the bottom of the base arm platform main shaft via a bearing seat; a driven gear is fixedly arranged in the middle of the rotating main arm shaft; the driven gear meshes with the annular central gear. The bottom of the ultrasonic mixing cylinder assembly for the preparation liquid is rotatably equipped with a rocker arm base assembly. At this time, the bottom of the main shaft of the bottom arm stage is rotatably connected to the far end of the support arm through a bearing seat. The ring-shaped central gear meshes with the three driven gears on the three rotating main arm shafts. The three support arms are located between the ultrasonic mixing cylinder for the preparation liquid and the ring-shaped rocker arm base frame. The three support arms are distributed in parallel. The bottom of the rotating main arm shaft is rotatably connected to the rocker arm side stage through a bearing seat. The ring-shaped central gear is driven to rotate by a drive motor.

[0008] Preferably, the mixing liquid shaking and stirring assembly includes a mixing liquid shaking and stirring rod, with a shaking ball head fixedly mounted on the top of the mixing liquid shaking and stirring rod, and a mixing liquid shaking and stirring support rod and an ultrasonic generator fixedly mounted on the bottom of the mixing liquid shaking and stirring rod; the top arm of the mixing liquid shaking and stirring rod is sleeved inside an inclined grip tube. In use, the shaking ball head moves omnidirectionally within the central ball seat of the top cover, and the top arm of the mixing liquid shaking and stirring rod is sleeved inside the inclined grip tube. The central ball seat of the top cover and the shaft of the top cover drive motor are coaxial, and the top cover drive motor drives the inclined grip tube to rotate. Simultaneously, through the cooperation of the above structures, the bottom of the mixing liquid shaking and stirring assembly achieves circular stirring within the ultrasonic mixing cylinder assembly of the preparation liquid. In this way, solution A and suspension B are thoroughly stirred and mixed. At the same time, the mixing liquid shaking and stirring support rod and the ultrasonic generator fixedly mounted on the bottom of the mixing liquid shaking and stirring rod, through the combined action of the mixing liquid shaking and stirring support rod and the ultrasonic generator, further ensure the mixing quality of solution A and suspension B.

[0009] Preferably, the preparatory liquid mixing cylinder assembly includes a preparatory liquid mixing cylinder, with a feeding hopper and a preparatory liquid outlet bottom pipe fixedly installed at the top and bottom of the preparatory liquid mixing cylinder, respectively. A pressure cap and an electromagnetic metering valve are respectively installed on the feeding hopper and the preparatory liquid outlet bottom pipe. A preparatory liquid mixing cylinder stirring motor is fixedly installed on one side of the top of the preparatory liquid mixing cylinder, and a preparatory liquid mixing cylinder stirring support rod is fixedly installed on the stirring shaft of the preparatory liquid mixing cylinder stirring motor. A pneumatic vent pipe and a preparatory liquid mixing cylinder high-pressure flushing pipe are fixedly installed at both ends of the inner side of the top of the preparatory liquid mixing cylinder, respectively. The air pressure vent pipe and aeration pipe are connected to an external high-pressure air supply tank. The high-pressure flushing pipe and top cover spray pipe of the preparative liquid mixing cylinder are connected to an external high-pressure liquid delivery pump. The stirring motor of the preparative liquid mixing cylinder drives the stirring support rod of the preparative liquid mixing cylinder to rotate, realizing the stirring treatment during the pre-preparation of solution A or suspension B. The air pressure vent pipe pressurizes the preparative liquid mixing cylinder, enabling the solution in the preparative liquid mixing cylinder to be smoothly discharged into the ultrasonic mixing cylinder of the preparation liquid. The high-pressure flushing pipe of the preparative liquid mixing cylinder enables high-pressure flushing when the preparative liquid mixing cylinder is idle. The preparative liquid mixing cylinder assembly has a preparative... A pre-mixing tank stirring support rod is fixedly installed on the stirring shaft of the liquid mixing tank. At both ends of the inner top of the pre-mixing tank, a pneumatic vent pipe and a pre-mixing tank high-pressure flushing pipe are fixedly installed respectively. The stirring motor drives the pre-mixing tank stirring support rod to rotate, realizing the stirring treatment during the pre-preparation of solution A or suspension B. The pneumatic vent pipe pressurizes the pre-mixing tank, enabling the solution in the pre-mixing tank to flow smoothly into the ultrasonic mixing tank of the preparation liquid. The high-pressure flushing pipe enables high-pressure flushing of the pre-mixing tank when it is idle.

[0010] Preferably, the shaking ball head moves omnidirectionally within the central ball seat of the top cover. This omnidirectional movement enables circular stirring, thereby achieving better and more thorough mixing of solution A and suspension B.

[0011] Preferably, the central ball seat of the top cover and the shaft of the top cover drive motor are coaxial.

[0012] Preferably, the air pressure vent pipe and aeration pipe are connected to an external high-pressure air delivery tank, and the high-pressure flushing pipe of the preparatory liquid mixing cylinder and the top cover spray pipe are connected to an external high-pressure liquid delivery pump.

[0013] Preferably, the plurality of support arms are located between the ultrasonic mixing cylinder of the preparation liquid and the annular rocker arm base, and the plurality of support arms are distributed in a parallel structure.

[0014] Preferably, the two sets of preparative liquid mixing cylinders at the top of the ultrasonic mixing cylinder assembly are used as naringin dissolving cylinders and octenyl succinic acid porous starch mixing cylinders, respectively; the preparation method of the octenyl succinic acid modified porous starch ester-loaded naringin complex includes the following steps.

[0015] (S01) Dissolve an appropriate amount of naringin in solvent I to prepare solution A;

[0016] (S02) Take an appropriate amount of octenyl succinic acid porous starch and add it to solvent II. After dispersing and mixing, suspension B is prepared.

[0017] (S03) The solution A in step (S01) and the suspension B in step (S02) are placed in an ultrasonic mixing device for ultrasonic mixing, and then centrifuged and dried to obtain the octenyl succinic acid modified porous starch ester loaded with naringin complex.

[0018] Preferably, the ultrasonic intensity is 10W to 300W. More preferably, the ultrasonic intensity is 50W to 250W. Even more preferably, the ultrasonic intensity is 100W to 200W. Limiting the ultrasonic intensity is to better ensure the load rate and better load-bearing effect.

[0019] Preferably, the ultrasound time is 15 min to 30 min. More preferably, the ultrasound time is 20 min to 25 min. The limitation on the ultrasound time is to balance timeliness and completeness of load.

[0020] The second technical solution of the present invention: a method for preparing octenyl succinic acid modified porous starch ester supported on naringin complex, comprising the following steps,

[0021] (S01) Dissolve an appropriate amount of naringin in solvent I to prepare solution A;

[0022] (S02) Take an appropriate amount of octenyl succinic acid porous starch and add it to solvent II. After dispersing and mixing, suspension B is prepared.

[0023] (S03) The solution A in step (S01) and the suspension B in step (S02) are placed in an ultrasonic mixing device for ultrasonic mixing, and then centrifuged and dried to obtain the octenyl succinic acid modified porous starch ester loaded with naringin complex.

[0024] This invention uses solvent I to dissolve naringin, facilitating subsequent loading. Octenyl succinic acid-modified porous starch ester is prepared by treating octenyl succinic acid porous starch with solvent II. Ultrasonic mixing improves the loading rate of naringin on the octenyl succinic acid-modified porous starch ester. This invention utilizes the loading and solubilizing properties of octenyl succinic acid-modified porous starch ester, along with its characteristics as a food additive, to serve as a loading carrier for naringin dissolved in solvent I, protecting naringin and inhibiting its decomposition in saliva and gastric juice. Naringin is released and exerts its effect only after being transported to the appropriate location within the organism, thus improving its utilization in vivo. This invention uses octenyl succinic acid-modified porous starch ester to protect and transport naringin, improving its bioavailability and expanding its applications in food, cosmetics, and pharmaceuticals. The octenyl succinic acid-modified porous starch ester prepared based on this invention can load naringin with a high loading rate. The resulting complex can significantly improve the solubility and in vivo bioavailability of naringin, expanding its application in the food, beverage, and pharmaceutical industries.

[0025] Preferably, solvent I is at least one of ethanol, methanol, or acetone. Since naringin has low solubility in water, choosing ethanol, methanol, or acetone can improve the solubility of naringin, facilitating better subsequent loading.

[0026] Preferably, solvent II is at least one of petroleum ether, n-heptane, or n-hexane; solvent II is used to disperse and esterify octenyl succinic acid porous starch, and the choice of petroleum ether, n-heptane, or n-hexane can better disperse and esterify octenyl succinic acid porous starch.

[0027] Preferably, the mass percentage of naringin in solution A is 0.2% to 2%. More preferably, the mass percentage of naringin in solution A is 0.5% to 1.5%. Even more preferably, the mass percentage of naringin in solution A is 0.8% to 1.2%. Even more preferably, the mass percentage of naringin in solution A is 1%. Limiting the mass percentage of naringin in solution A allows for better loading of naringin and ensures sufficient loading.

[0028] Preferably, the mass ratio of octenyl succinic acid porous starch to naringin is 2–10. More preferably, the mass ratio of octenyl succinic acid porous starch to naringin is 4–8. Even more preferably, the mass ratio of octenyl succinic acid porous starch to naringin is 5–7. Limiting the mass ratio of octenyl succinic acid porous starch to naringin can better improve the loading capacity, resulting in a final complex with better in vivo bioavailability.

[0029] Preferably, the degree of esterification of the octenyl succinic acid porous starch is 0.001 to 0.03. More preferably, the degree of esterification of the octenyl succinic acid porous starch is 0.005 to 0.02. Even more preferably, the degree of esterification of the octenyl succinic acid porous starch is 0.01 to 0.015. Limiting the degree of esterification of the octenyl succinic acid porous starch is to ensure the safety of the final composite when it functions in vivo.

[0030] Preferably, the pore size of the octenyl succinic acid porous starch particles is 50–1000 nm. More preferably, the pore size of the octenyl succinic acid porous starch particles is 100–800 nm. Even more preferably, the pore size of the octenyl succinic acid porous starch particles is 300–600 nm. The octenyl succinic acid porous starch is used to load naringin; limiting the pore size of the octenyl succinic acid porous starch particles is for better adsorption of naringin particles.

[0031] Preferably, the ultrasonic intensity is 10W to 300W. More preferably, the ultrasonic intensity is 50W to 250W. Even more preferably, the ultrasonic intensity is 100W to 200W. Limiting the ultrasonic intensity is to better ensure the load rate and better load-bearing effect.

[0032] Preferably, the ultrasound time is 15 min to 30 min. More preferably, the ultrasound time is 20 min to 25 min. The limitation on the ultrasound time is to balance timeliness and completeness of load.

[0033] Preferably, the volume ratio of suspension B to solution A is 0.2 to 3.0. More preferably, the volume ratio of suspension B to solution A is 0.5 to 2.5. More preferably, the volume ratio of suspension B to solution A is 1 to 2. More preferably, the volume ratio of suspension B to solution A is 1.2 to 1.7. Limiting the volume ratio of suspension B to solution A is also to ensure a better loading effect of octenyl succinic acid modified porous starch ester on naringin.

[0034] The present invention has the following beneficial effects:

[0035] (1) Solvent I was used to dissolve naringin to facilitate subsequent loading work; octenyl succinic acid modified porous starch ester was prepared by treating octenyl succinic acid porous starch with solvent II; ultrasonic mixing can improve the loading rate of naringin on octenyl succinic acid modified porous starch ester.

[0036] (2) Using the loading and solubilizing properties of octenyl succinic acid-modified porous starch ester, and its own characteristics as a food additive, it is used as a loading carrier for naringin after it is dissolved in solvent I, to protect naringin, inhibit its decomposition in saliva and gastric juice, and release it to play a role only after naringin is transported to the appropriate location in the body, thereby improving the utilization of naringin in the body.

[0037] (3) By modifying porous starch ester with octenyl succinic acid to protect and transport naringin, its bioavailability can be improved and its application in food, cosmetics and pharmaceuticals can be expanded. Based on the octenyl succinic acid modified porous starch ester prepared by the present invention, naringin can be loaded with a high loading rate. The loaded complex can significantly improve the solubility and in vivo bioavailability of naringin, which will expand the application of naringin in the food, beverage and pharmaceutical industries.

[0038] (4) When in use, two sets of preparative liquid mixing cylinders are fixedly installed on both sides of the top of the ultrasonic mixing cylinder assembly of the preparation liquid. The two sets of preparative liquid mixing cylinders on the top of the ultrasonic mixing cylinder assembly of the preparation liquid are used as naringin dissolving cylinders and octenyl succinic acid porous starch mixing cylinders, respectively. Naringin and acetone are added to one set of preparative liquid mixing cylinders and stirred to prepare solution A. Octenyl succinic acid porous starch and petroleum ether are added to the other set of preparative liquid mixing cylinders and stirred to prepare suspension B. Then, solution A and suspension B in the two sets of preparative liquid mixing cylinders are added to the ultrasonic mixing cylinder assembly of the preparation liquid in proportion. At this time, a mixing top cover assembly is set on the top of the ultrasonic mixing cylinder assembly of the preparation liquid, and a mixing shaking and stirring assembly is set on the mixing top cover assembly. An aeration pipe is fixedly installed at the bottom of the ultrasonic mixing cylinder of the preparation liquid. The aeration pipe is connected to the external high-pressure air supply tank. Through the high-pressure air supply of the aeration pipe, the liquid at the bottom of the ultrasonic mixing cylinder of the preparation liquid is rolled upward, which further ensures that the solution inside the ultrasonic mixing cylinder of the preparation liquid is fully mixed from top to bottom.

[0039] (5) The ultrasonic mixing cylinder assembly of the preparation liquid and the mixing cylinder assembly of the preparative liquid are made to move in a circular motion on the rocker base assembly. Through this external shaking drive, the liquid inside the ultrasonic mixing cylinder assembly of the preparation liquid and the mixing cylinder assembly of the preparative liquid are further mixed. Through this external shaking mixing and mixing inside the cylinder, the two-stage preparation solutions are fully mixed. In this way, the full and efficient mixing during the preparation of porous starch ester loaded with naringin is guaranteed.

[0040] (6) The aeration pipe is connected to the external high-pressure air supply tank. Through the high-pressure air supply of the aeration pipe, the liquid at the bottom of the preparation liquid ultrasonic mixing cylinder is rolled upward, ensuring that the solution inside the preparation liquid ultrasonic mixing cylinder is fully mixed. Attached Figure Description

[0041] Figure 1The in vitro release curves of naringin in the octenyl succinic acid modified porous starch ester-supported naringin complexes prepared in Examples 1 to 3 are shown.

[0042] Figure 2 The in vivo bioavailability curves of the octenyl succinic acid modified porous starch ester loaded with naringin prepared in Examples 1 to 3 are shown.

[0043] Figure 3 This is a perspective view of the present invention;

[0044] Figure 4 This is a perspective view of the invention from another angle;

[0045] Figure 5 This is an exploded view of the present invention;

[0046] Figure 6 This is a perspective view of the ultrasonic mixing cylinder assembly and rocker arm base assembly for preparing the liquid according to the present invention;

[0047] Figure 7 This is a perspective view of the ultrasonic mixing cylinder assembly and rocker arm base assembly of the preparation liquid of the present invention from another angle.

[0048] Figure 8 This is a perspective view of the mixing top cover assembly and the mixing liquid shaking and stirring assembly of the present invention;

[0049] Figure 9 This is an exploded view of the mixing top cover assembly and the mixing liquid shaking and stirring assembly of the present invention;

[0050] Figure 10 This is an exploded view of the mixing top cover assembly and the mixing liquid shaking and stirring assembly of the present invention from another perspective.

[0051] Figure 11 This is a perspective view of the preparative liquid mixing cylinder assembly of the present invention;

[0052] Figure 12 This is a cross-sectional view of the preparative liquid mixing cylinder assembly of the present invention.

[0053] The labels in the attached diagram are as follows: 100-Ultrasonic mixing cylinder assembly for preparation liquid; 101-Ultrasonic mixing cylinder for preparation liquid; 102-Bottom arm of mixing cylinder; 103-Reinforcing rib plate; 104-Main shaft of bottom arm; 105-Aeration pipe; 106-Liquid pump; 200-Mixing top cover assembly; 201-Mixing top cover; 202-Top cover frame plate; 203-Top cover drive motor; 204-Supporting side plate; 205-Inclined grip pipe; 206-Top cover spray pipe; 207-Center ball seat of top cover; 208-Vent pipe; 300-Rock arm base assembly; 301-Annular rocker arm base frame; 302-Rock arm side plate; 303-Frame support leg; 304-First support rod; 305-First fixing plate; 306-Drive motor; 307-Annular... 308-Second fixed plate; 309-Second support rod; 310-Rotating main arm shaft; 311-Support arm; 312-Driven gear; 400-Mixed liquid shaking and stirring assembly; 401-Mixed liquid shaking and stirring rod; 402-Shaking ball head; 403-Mixed liquid shaking and stirring support rod; 404-Ultrasonic generator; 500-Prepared liquid mixing cylinder assembly; 501-Prepared liquid mixing cylinder; 502-Prepared liquid outlet bottom pipe; 503-Solenoid metering valve; 504-Air pressure vent pipe; 505-Prepared liquid mixing cylinder high-pressure flushing pipe; 506-Prepared liquid mixing cylinder stirring motor; 507-Feeding hopper; 508-Closure cap; 509-Prepared liquid mixing cylinder stirring shaft; 510-Prepared liquid mixing cylinder stirring support rod. Detailed Implementation

[0054] The following will describe in detail the implementation of this application with reference to the accompanying drawings and embodiments, so that the implementation process of how this application uses technical means to solve technical problems and achieve technical effects can be fully understood and implemented accordingly.

[0055] like Figure 3 The apparatus shown is for preparing octenyl succinic acid-modified porous starch ester-supported naringin complex, including as follows: Figure 4 The ultrasonic mixing cylinder assembly 100 for the preparation liquid shown has a top portion provided with, as shown in the image. Figure 8 The mixing top cover assembly 200 shown has a mixing liquid shaking and stirring assembly 400 mounted on it; the bottom of the preparation liquid ultrasonic mixing cylinder assembly 100 is rotatably equipped with... Figure 6 The rocker arm base assembly 300 and the ultrasonic mixing cylinder assembly 100 of the preparation liquid are shown, both sides of the top are fixedly provided with, as shown in the figure. Figure 5The preparative liquid mixing cylinder assembly 500 is shown; the preparation liquid ultrasonic mixing cylinder assembly 100 includes a preparation liquid ultrasonic mixing cylinder 101, a plurality of mixing cylinder bottom arm platforms 102 are fixedly installed on the preparation liquid ultrasonic mixing cylinder 101 by reinforcing ribs 103, and a bottom arm platform main shaft 104 is fixedly installed on the mixing cylinder bottom arm platform 102; an aeration pipe 105 is fixedly installed at the bottom end inside the preparation liquid ultrasonic mixing cylinder 101, and a liquid pump 106 is fixedly installed on the outer side of the bottom of the preparation liquid ultrasonic mixing cylinder 101.

[0056] The hybrid top cover assembly 200 includes a hybrid top cover 201, and the hybrid top cover 201 has a fixedly disposed interior thereof. Figure 10 The top cover spray pipe 206 shown is provided with a top cover frame plate 202, a vent pipe 208 and a top cover center ball seat 207 fixedly installed on the top of the mixing top cover 201. A top cover drive motor 203 is fixedly installed on the top of the top cover frame plate 202. An inclined grip pipe 205 is fixedly installed on the shaft of the top cover drive motor 203 through a support side plate 204.

[0057] The rocker arm base assembly 300 includes an annular rocker arm base frame 301. Multiple rocker arm side platforms 302 are symmetrically distributed and fixedly mounted on the edge of the annular rocker arm base frame 301. Support legs 303 are fixedly mounted on the bottom of each rocker arm side platform 302. A first fixing plate 305 is fixedly mounted inside the annular rocker arm base frame 301 via a first support rod 304. A support leg 303 is fixedly mounted at the bottom of the first fixing plate 305. Figure 7 The drive motor 306 shown has a second fixed disk 308 fixedly mounted on its shaft. A ring-shaped central gear 307 is fixedly mounted on the outside of the second fixed disk 308 via a second support rod 309. A rotating main arm shaft 310 is rotatably mounted on the top of the rocker arm side platform 302 via a bearing seat. A support arm 311 is fixedly mounted on the top of the rotating main arm shaft 310. The distal end of the support arm 311 is rotatably connected to the bottom of the bottom arm platform main shaft 104 via a bearing seat. A driven gear 312 is fixedly mounted in the middle of the rotating main arm shaft 310. The driven gear 312 meshes with the ring-shaped central gear 307.

[0058] The mixing stirring assembly 400 includes a mixing stirring rod 401, and a top of the mixing stirring rod 401 is fixedly provided with a... Figure 9 The shaking ball head 402 shown has a mixing liquid shaking stirring rod 401 with a mixing liquid shaking stirring support rod 403 and an ultrasonic generator 404 fixedly installed at the bottom; the top arm of the mixing liquid shaking stirring rod 401 is sleeved in the inclined grip tube 205.

[0059] The preparative liquid mixing cylinder assembly 500 includes, for example: Figure 11The prepared liquid mixing cylinder 501 shown has a feeding hopper 507 and a prepared liquid outlet bottom pipe 502 fixedly installed at its top and bottom, respectively. A clamping cap 508 and a solenoid metering valve 503 are respectively installed on the feeding hopper 507 and the prepared liquid outlet bottom pipe 502. A prepared liquid mixing cylinder stirring motor 506 is fixedly installed on one side of the top of the prepared liquid mixing cylinder 501. A stirring shaft 509 of the prepared liquid mixing cylinder stirring motor 506 is fixedly equipped with... Figure 12 The prepared liquid mixing cylinder stirring support rod 510 shown is provided with a pneumatic vent pipe 504 and a prepared liquid mixing cylinder high-pressure flushing pipe 505 respectively fixed at both ends of the inner side of the top of the prepared liquid mixing cylinder 501.

[0060] The rocking ball head 402 moves omnidirectionally within the central ball seat 207 of the top cover. The central ball seat 207 of the top cover and the top cover drive motor 203 are coaxial. The air pressure vent pipe 504 and the aeration pipe 105 are connected to an external high-pressure air supply tank, and the high-pressure flushing pipe 505 of the preparative liquid mixing cylinder and the top cover spray pipe 206 are connected to an external high-pressure liquid delivery pump. Multiple support arms 311 are located between the ultrasonic mixing cylinder 101 of the preparative liquid and the annular rocker arm base 301, and the multiple support arms 311 are distributed in a parallel structure. The two sets of preparative liquid mixing cylinder assemblies 500 at the top of the ultrasonic mixing cylinder assembly 100 of the preparative liquid are used as naringin dissolving cylinders and octenyl succinic acid porous starch mixing cylinders, respectively.

[0061] A method for preparing an octenyl succinic acid-modified porous starch ester-supported naringin complex includes the following steps:

[0062] (S01) Dissolve an appropriate amount of naringin in solvent I to prepare solution A; solvent I is at least one of ethanol, methanol or acetone; the mass percentage of naringin in solution A is 0.2% to 2%; the pore size of the octenyl succinic acid porous starch particles is 50 to 1000 nm; octenyl succinic acid porous starch is used to load naringin.

[0063] (S02) Take an appropriate amount of octenyl succinic acid porous starch and add it to solvent II. After dispersing and mixing, a suspension B is prepared. Solvent II is at least one of petroleum ether, n-heptane, or n-hexane. Solvent II is used to disperse OSA esterified porous starch. The boiling point of petroleum ether is 60℃. The mass ratio of octenyl succinic acid porous starch to naringin is 2-10. The degree of esterification of octenyl succinic acid porous starch is 0.001-0.03.

[0064] (S03) The solution A from step (S01) and the suspension B from step (S02) are placed in an ultrasonic mixing device for ultrasonic mixing, and then centrifuged and dried to obtain the octenyl succinic acid modified porous starch ester supported naringin complex; the ultrasonic intensity is 10W to 300W; the ultrasonic time is 15min to 30min; the ultrasonic time is 20min to 25min; the volume ratio of suspension B to solution A is 0.2 to 3.0.

[0065] This invention, through experimental research, derives the process steps and preparation method for loading naringin onto porous starch ester modified with octenyl succinic acid. Specifically, it determines that the naringin solution and the porous starch suspension modified with octenyl succinic acid are ultrasonically mixed. The quality of the ultrasonic mixing is the basis and prerequisite for the preparation of this invention. Subsequently, the ultrasonically mixed solution is centrifuged and dried to obtain the octenyl succinic acid modified porous starch ester loaded with naringin. The preparation process steps and proportions are further defined. Experimental research shows that the mass percentage of naringin is 0.2%–2%. The composite prepared with a mass ratio of 2-10 for octenyl succinic acid porous starch and naringin, a degree of esterification of octenyl succinic acid porous starch of 0.001-0.03, and a particle surface pore size of 50-1000 nm, can significantly improve the solubility and bioavailability of naringin. Furthermore, under a preparation environment with an ultrasonic intensity of 10W-300W and an ultrasonic time of 15-30 minutes, the composite prepared exhibits a high loading rate of naringin on the octenyl succinic acid-modified porous starch ester.

[0066] This invention provides a method for preparing an octenyl succinic acid-modified porous starch ester loaded with naringin, belonging to the field of pharmaceutical and health food technology. This invention prepares the octenyl succinic acid-modified porous starch ester loaded with naringin based on an antisolvent precipitation method. The method of this invention is simple and efficient. The octenyl succinic acid-modified porous starch ester prepared based on this invention can load naringin with a high loading rate. The resulting complex can significantly improve the solubility and in vivo bioavailability of naringin.

[0067] Example 1:

[0068] Solution A was prepared by dissolving 0.4% naringin (NA) in acetone. Five times the mass (compared to the mass of naringin) of octenyl succinic acid porous starch (esterification degree of 0.010-0.019, particle surface pore size of approximately 350 nm) was added and dispersed in petroleum ether (boiling point of 60℃) and mixed evenly to prepare suspension B. Solution A and suspension B were thoroughly mixed under ultrasonic conditions (ultrasonic intensity of 200 W, ultrasonic time of 20 min) (volume ratio of suspension B to solution A was 0.6). The mixture was centrifuged and the precipitate was dried to obtain the octenyl succinic acid modified porous starch ester-supported naringin complex (abbreviated as OSAPS-NA complex).

[0069] Example 2:

[0070] In this embodiment, five times the mass of starch (compared to the mass of naringin (NA)) was added and dispersed in petroleum ether (boiling point 60°C) and mixed evenly to prepare suspension B. The remaining steps were the same as in Example 1, thus obtaining the unmodified starch-loaded naringin complex (abbreviated as UMS-NA complex).

[0071] Example 3:

[0072] In this embodiment, 5 times the mass of porous starch (compared to the mass of naringin (NA)) was added and dispersed in petroleum ether (boiling point 60°C) and mixed evenly to prepare suspension B. The remaining steps were the same as in Example 1, thus obtaining the porous starch-loaded naringin complex (abbreviated as PS-NA complex).

[0073] Experimental Test A: Determination of Encapsulation Efficiency and Drug Loading Rate

[0074] (I) Methods for determining encapsulation efficiency and drug loading

[0075] Encapsulation efficiency (EE) and drug loading (DL) are key factors for evaluating the encapsulation effect of the carrier. The DL and EE of the OSAPS-NA complex and the PS-NA complex were calculated using formulas (1) and (2), respectively.

[0076]

[0077]

[0078] In the above formula, M t The total mass (mg) of naringin, V s C represents the volume of the supernatant (mL). s M represents the concentration of naringin in the supernatant (mg / mL). pThe mass of the precipitate is expressed in mg. The concentration of naringin was determined by high-performance liquid chromatography (HPLC). The detection conditions were as follows: C18 reversed-phase column (5 μm, 250 mm × 4.6 mm); detection temperature: 30 °C; mobile phase: acetonitrile-water (v / v = 20 / 80); flow rate: 1.0 mL / min; detection wavelength: 283 nm; injection volume: 10 μL.

[0079] (II) Measurement Results

[0080] The results showed that the EE and DL values ​​of the OSAPS-NA complex were 13.57±1.75% and 78.51±3.84%, respectively; the EE and DL values ​​of the UMS-NA complex were 0.29±0.03% and 14.57±2.03%, respectively; and the EE and DL values ​​of the PS-NA complex were 13.07±1.72% and 75.22±4.83%, respectively. These results indicate that both OSAPS and PS can efficiently adsorb NA nanoparticles, while UMS has a weaker adsorption capacity for NA. Furthermore, there was no significant difference between the OSAPS-NA complex and the PS-NA complex, suggesting that under the current preparation process conditions, the adsorption efficiency of OSAPS for NA is similar to that of PS.

[0081] Experimental Detection B: Determination of the Saturated Solubility of the Complex

[0082] (I) Method for determining the saturated solubility of naringin in the complex

[0083] Octenyl succinic acid-modified porous starch esters prepared using the methods described in Examples 1 to 3 are simple and efficient. Excess solids of the three complexes were added to simulated gastric juice, simulated intestinal juice, and deionized water, respectively, and stirred at 100 rpm for 24 hours. The resulting samples were centrifuged at 10,000 rpm for 10 minutes, and 10 μL of the supernatant was diluted and injected into an HPLC system for analysis. The concentrations and standard deviations (n ​​= 3) of NA in the three complexes in the three media, i.e., the saturated solubility of naringin in the three complexes (37℃), were compiled, and the results are shown in Table 1.

[0084] Table 1: Saturated solubility of naringin in the three complexes

[0085]

[0086] Compared with NA raw material, *p<0.05, **p<0.01. NA, naringin; UMS, native starch; PS, porous starch; OSAPS-NA, octenyl succinic acid modified porous starch ester.

[0087] (II) Measurement Results

[0088] Table 1 shows that, compared to the raw naringin, the PS-NA and OSAPS complexes exhibited significantly improved saturated solubility of naringin in deionized water, simulated gastric fluid, and simulated intestinal fluid. The OSAPS-NA complex showed significantly higher saturated solubility of NA compared to the other complexes. Compared to the raw NA, the saturated solubility of NA in the OSAPS-NA and PS-NA complexes in simulated gastric fluid, simulated intestinal fluid, and deionized water increased by approximately 7.08–21.66 times. This is likely due to the low crystallinity of NA, the nano-effect, and the combined effect of intermolecular forces between NA and PS (or OSAPS). In deionized water, the saturated solubility of the OSAPS-NA complex was 3.05 times that of the PS-NA complex, possibly because the amphiphilic structure of OSAPS enhances the water solubility of the hydrophobic component of naringin. Furthermore, the saturated solubility of NA in the UMS-NA complex increased significantly in all three media, but it was still lower than that of the other two complexes. This indicates that UMS does not have a strong solubilizing effect on NA, but the nanoscale size of some NA particles does play a solubilizing role.

[0089] Experimental detection of the in vitro release pattern of complex C:

[0090] (I) Methods for determining release patterns

[0091] 1. Solution preparation

[0092] Preparation of simulated saliva: Add α-amylase to a sodium carbonate-sodium bicarbonate buffer solution with a pH of 7.0±0.2 to make the amylase concentration 250 U / mL, and mix well to obtain simulated saliva.

[0093] Preparation of simulated gastric juice: Add concentrated hydrochloric acid to 1000 mL of deionized water to make the pH of the hydrochloric acid solution 1.5 ± 0.2. Add 10.0 g of pepsin to the hydrochloric acid solution and mix well to obtain simulated gastric juice.

[0094] Preparation of simulated intestinal fluid: Dissolve 6.8 g of potassium dihydrogen phosphate in 1000 mL of deionized water, and then adjust the pH of the solution to 6.8 with 0.1 mol / L sodium hydroxide. Add 10.0 g of trypsin and 10.0 g of sodium bile acid hydrate to the above solution, and mix well to obtain simulated intestinal fluid.

[0095] 2. Study on release patterns

[0096] NA raw material, UMS-NA complex, PS-NA complex, and OSAPS-NA complex (containing the same mass of NA) were added to 5 mL of simulated saliva, stirred at 37°C for 5 min, and then 50 mL of simulated gastric juice was introduced into the saliva digest. After stirring for 2 h, 25 mL of simulated intestinal juice was added to the gastric digest and stirred for another 2 h. 200 μL samples were taken at 2, 5, 10, 30, 60, 90, 120, 240, and 360 min, and an equal volume of simulated digestion solution was added to the system at the same time. The samples were diluted with 4 times the volume of anhydrous ethanol, mixed thoroughly, and the concentration of NA in the samples at each time point was detected by high performance liquid chromatography. The cumulative release amount of NA at each time point was calculated using the concentration detection results of the above samples. The formula for calculating the cumulative release rate of NA at each time point is shown in equation (3). The curve plotted with time as the abscissa and the cumulative release rate as the ordinate is the release curve of NA raw material and OSAPS-NA complex.

[0097]

[0098] Where C r V represents the cumulative release rate (%) of NA in the release system over time t; e This is the sample volume taken each time; V0 is the volume of solution released; C i and C n , respectively, are the concentrations of NA in samples i and n.

[0099] (II) Measurement Results

[0100] The release of naringin from the three complexes in Examples 1-3 was detected. These results provide guidance for effectively expanding the application of the OSAPS-NA complex in industrial production. The release of naringin from the three complexes is as follows: Figure 1 As shown. Under the same simulated digestion system, the release curves of NA from the NA precursor and the three complexes are as follows. Figure 1 As shown in the figure, the final cumulative release rates of NA in the NA raw material, UMS-NA complex, PS-NA complex, and OSAPS-NA complex within 6 hours were 34.61±0.96%, 43.29±2.31%, 85.35±2.40%, and 82.16±2.04%, respectively. This indicates that the solubility of NA in the simulated digestion system increased significantly by 1.25, 2.49, and 2.37 times after loading with UMS, PS, and OSAPS, respectively. The UMS-NA complex exhibited low encapsulation efficiency and poor encapsulation effect. Therefore, the increased cumulative release of NA in the UMS-NA complex is primarily due to the nanoscale size of the NA particles. The results for the OSAPS-NA and PS-NA complexes may be attributed to the combined effect of small particle size and hydrogen bonding.

[0101] Experimental Detection D: Determination of the bioavailability of the complex

[0102] (I) Methods for determining the bioavailability of the complex in vivo

[0103] Eighteen Sprague-Dawley rats (SD rats) weighing between 250 ± 20 g were selected for the bioavailability study. The SD rats were randomly divided into two groups (NA raw material group and OSAPS-NA complex group) and housed under identical external environmental conditions (including temperature, humidity, light, food, and water) for three days, and fasted for 24 hours before oral administration. The three groups of rats (n = 6) were orally administered deionized water or a NA raw material-deionized water suspension (OSAPS-NA suspension) (oral dose calculated based on NA, 50 mg / kg by gavage), respectively. Following oral administration, blood samples were obtained via orbital venous sinus puncture at time points of 0, 5, 15, 30, 60, 90, 120, 240, 360, 720, 1440, and 2880 min. These blood samples were immediately centrifuged (3000 r / min, 10 min) and stored at -40°C. The Laboratory Animal Welfare and Ethics Committee of Hangzhou Herbe Technology Co., Ltd. reviewed and approved the animal experimental procedures for this study (License No.: HB2021130009031WL-A).

[0104] Prior to analysis, frozen samples were thawed in a 37°C water bath. 20 μL of serum sample was precisely pipetted and extracted with 100 μL of anhydrous ethanol. The extraction system was vortexed for 1 min and then centrifuged (10000 rpm, 10 min). Finally, 10 μL of the supernatant was injected into the HPLC system for detection. A drug-time curve was plotted with serum NA concentration on the ordinate and time on the abscissa. Pharmacokinetic parameters of NA in the NA precursor and OSAPS-NA complex in rats were fitted and calculated using a compartmental model and a statistical moment model. Significance of the experimental results was evaluated using one-way ANOVA.

[0105] (II) Measurement Results

[0106] The pharmaco-time curves of NA raw materials, PS-NA complex, and OSAPS-NA complex are as follows: Figure 2 As shown in Table 2, the pharmacokinetic parameters of the oral NA raw material and the OSAPS-NA complex in rats conform to a two-compartment model, and the bimodal distribution of the pharmacokinetic curves indicates the presence of enterobiliary circulation during NA elimination in rats. Based on the fitting results, the pharmacokinetic parameters of the NA raw material and the OSAPS-NA complex are shown in Table 2. Figure 2 As shown in Table 2, the peak plasma concentration C of the OSAPS-NA complex is... maxand the area under the drug-time curve (AUC) from 0 to 2880 min (0～2880) The C values ​​are approximately 4.57 and 7.79 times those of the NA raw material; the C of the PS-NA complex... max and AUC (0～2880) The values ​​are approximately 5.25 and 10.14 times that of the NA raw material, respectively. The relative bioavailability (F) of the OSAPS-NA complex and the PS-NA complex... rb The concentrations of NA in the OSAPS-NA complex were 1014.42 ± 57.78 and 979.61 ± 56.79 μg / L·min, respectively. These key parameters indicate that both complexes significantly improved the relative bioavailability of NA in rats. Compared with the PS-NA complex, the OSAPS-NA complex showed a significantly higher time to peak concentration (T0) of NA. max The distribution half-life (t0.05) was significantly earlier, indicating that the nanoparticles of NA in the OSAPS-NA complex entered the bloodstream through the blood vessel wall in rats in a shorter time. However, the distribution half-life (t0.05) was longer. 1 / 2α This indicates that the NA in this complex is distributed to tissues and organs relatively slowly, which also explains the high mean retention time (MRT) of the OSAPS-NA complex. (0-∞) ) and the area under the drug-time curve (AUC) from 0 to ∞ min (0-∞) This result indicates that the preparation of the OSAPS-NA complex can indeed significantly improve the oral relative bioavailability of naringin.

[0107] Table 2. Pharmacokinetic parameters of the oral NA raw material and OSAPS-NA complex (n=6) 1

[0108] parameter unit NA raw materials PS-NA complex OSAPS-NA complex <![CDATA[C max ]]> μg / mL 0.88±0.02 <![CDATA[4.62±0.51 ** ]]> 4.02±0.50** <![CDATA[T max ]]> min 60.00±0.00 60.00±0.01 15.00±0.00** <![CDATA[t 1 / 2α ]]> min 34.96±2.21 <![CDATA[29.57±3.26 * ]]> 69.32±2.07* <![CDATA[t 1 / 2β ]]> min 69.31±3.47 69.31±2.37 69.32±3.59 <![CDATA[AUC (0-2880) ]]> μg / L·min 636.15±25.20 <![CDATA[6453.21±97.39 ** ]]> 6231.82±100.11** <![CDATA[AUC (0-∞) ]]> μg / L·min 1553.07±177.87 <![CDATA[15290.57±234.90 ** ]]> 27398.23±261.10** <![CDATA[MRT (0–∞) ]]> min 5566.07±186.95 5200.33±187.49 11129.46±239.65** <![CDATA[F rb ]]> ％ - 1014.42±57.78 979.61±56.79

[0109] 1 Compared to NA raw materials, * p<0.05, ** p<0.01.

[0110] An apparatus for preparing octenyl succinic acid modified porous starch ester loaded with naringin complex includes a preparation liquid ultrasonic mixing cylinder assembly 100, a mixing top cover assembly 200 on the top of the preparation liquid ultrasonic mixing cylinder assembly 100, and a rocker arm base assembly 300 rotatably mounted on the bottom of the preparation liquid ultrasonic mixing cylinder assembly 100. Two preparatory liquid mixing cylinder assemblies 500 are fixedly mounted on both sides of the top of the preparation liquid ultrasonic mixing cylinder assembly 100. The two preparatory liquid mixing cylinder assemblies 500 on the top of the preparation liquid ultrasonic mixing cylinder assembly 100 are used as naringin dissolving cylinders and octenyl succinic acid porous starch mixing cylinders, respectively. A mixing liquid shaking and stirring assembly 400 is mounted on the mixing top cover assembly 200.

[0111] The ultrasonic mixing cylinder assembly 100 for the preparation liquid includes an ultrasonic mixing cylinder 101 for the preparation liquid. Three mixing cylinder bottom arm platforms 102 are fixedly installed on the ultrasonic mixing cylinder 101 by reinforcing ribs 103. A bottom arm platform main shaft 104 is fixedly installed on the mixing cylinder bottom arm platform 102. An aeration pipe 105 is fixedly installed at the bottom of the ultrasonic mixing cylinder 101, and a liquid pump 106 is fixedly installed on the outside of the bottom of the ultrasonic mixing cylinder 101. The aeration pipe 105 is connected to an external high-pressure air supply tank. Through the high-pressure air supply of the aeration pipe 105, the liquid at the bottom of the ultrasonic mixing cylinder 101 is rolled upward, ensuring that the solution inside the ultrasonic mixing cylinder 101 is fully mixed from top to bottom.

[0112] The mixing top cover assembly 200 includes a mixing top cover 201. A top cover spray pipe 206 is fixedly installed inside the mixing top cover 201. A top cover frame plate 202, a vent pipe 208, and a top cover center ball seat 207 are fixedly installed on the top of the mixing top cover 201. A top cover drive motor 203 is fixedly installed on the top of the top cover frame plate 202. An inclined grip pipe 205 is fixedly installed on the shaft of the top cover drive motor 203 through a support side plate 204.

[0113] The rocker arm base assembly 300 includes an annular rocker arm base frame 301. Three rocker arm side platforms 302 are symmetrically distributed and fixedly mounted on the edge of the annular rocker arm base frame 301. Support legs 303 are fixedly mounted on the bottom of the rocker arm side platforms 302. A first fixed plate 305 is fixedly mounted inside the annular rocker arm base frame 301 via a first support rod 304. A drive motor 306 is fixedly mounted on the bottom of the first fixed plate 305. A second fixed plate 308 is fixedly mounted on the shaft of the drive motor 306. An annular central gear 307 is fixedly mounted on the outside of the second fixed plate 308 via a second support rod 309. The top of the rocker arm side platform 302 is rotatably mounted with a rotating main arm shaft 310 via a bearing seat. A support arm 311 is fixedly mounted on the top of the rotating main arm shaft 310, and a driven gear 312 is fixedly mounted in the middle of the rotating main arm shaft 310. The bottom of the bottom arm platform main shaft 104 is rotatably connected to the far end of the support arm 311 via a bearing seat. The ring-shaped central gear 307 meshes with the three driven gears 312 on the three rotating main arm shafts 310. The three support arms 311 are located between the ultrasonic mixing cylinder 101 of the preparation liquid and the ring-shaped rocker arm base platform 301, and the three support arms 311 are distributed in parallel.

[0114] The mixing liquid shaking and stirring assembly 400 includes a mixing liquid shaking and stirring rod 401, a shaking ball head 402 fixedly installed at the top of the mixing liquid shaking and stirring rod 401, and a mixing liquid shaking and stirring support rod 403 and an ultrasonic generator 404 fixedly installed at the bottom of the mixing liquid shaking and stirring rod 401. The shaking ball head 402 can move in all directions within the central ball seat platform 207 of the top cover. The top arm of the mixing liquid shaking and stirring rod 401 is sleeved in the inclined grip tube 205. The central ball seat platform 207 of the top cover and the top cover drive motor 203 are coaxial.

[0115] The preparatory liquid mixing cylinder assembly 500 includes a preparatory liquid mixing cylinder 501. A feeding hopper 507 and a preparatory liquid outlet bottom pipe 502 are fixedly installed at the top and bottom of the preparatory liquid mixing cylinder 501, respectively. A clamping cap 508 and a solenoid metering valve 503 are respectively installed on the feeding hopper 507 and the preparatory liquid outlet bottom pipe 502. A preparatory liquid mixing cylinder stirring motor 506 is fixedly installed on one side of the top of the preparatory liquid mixing cylinder 501. A preparatory liquid mixing cylinder stirring support rod 510 is fixedly installed on the stirring shaft 509 of the preparatory liquid mixing cylinder 506. A pneumatic vent pipe 504 and a preparatory liquid mixing cylinder high-pressure valve are fixedly installed at both ends of the inner side of the top of the preparatory liquid mixing cylinder 501, respectively. The flushing pipe 505, the air pressure vent pipe 504, and the aeration pipe 105 are connected to an external high-pressure air supply tank. The high-pressure flushing pipe 505 of the preparatory liquid mixing cylinder and the top cover spray pipe 206 are connected to an external high-pressure liquid delivery pump. The stirring motor 506 of the preparatory liquid mixing cylinder drives the stirring support rod 510 of the preparatory liquid mixing cylinder to rotate, realizing the stirring treatment during the preparation of solution A or suspension B. The air pressure vent pipe 504 pressurizes the preparatory liquid mixing cylinder 501, realizing the smooth flow of the solution in the preparatory liquid mixing cylinder 501 into the ultrasonic mixing cylinder 101 of the preparation liquid. The high-pressure flushing pipe 505 of the preparatory liquid mixing cylinder realizes the high-pressure flushing of the preparatory liquid mixing cylinder 501 when it is idle.

[0116] The ultrasonic generator 404 in this invention is a known technology that has been widely used in daily life and is already disclosed. Its model number is CE-9600.

[0117] The working principle of this invention is as follows:

[0118] In this invention, two preparatory liquid mixing cylinder assemblies 500 are fixedly installed on both sides of the top of the ultrasonic mixing cylinder assembly 100. The two sets of preparatory liquid mixing cylinder assemblies 500 on the top of the ultrasonic mixing cylinder assembly 100 serve as a naringin dissolving cylinder and an octenyl succinic acid porous starch mixing cylinder, respectively. One set of preparatory liquid mixing cylinder assemblies 500 contains naringin and acetone and is stirred to prepare solution A. The other set of preparatory liquid mixing cylinder assemblies 500 contains octenyl succinic acid porous starch and petroleum ether and is stirred to prepare suspension B. Then, solutions A and suspension B from the two sets of preparatory liquid mixing cylinder assemblies 500 are added to the ultrasonic mixing cylinder assembly 100 in a specific ratio. At this time, a mixing top cover assembly 200 is installed on the top of the ultrasonic mixing cylinder assembly 100. A mixing liquid shaking and stirring assembly 400 is provided on the top cover assembly 200. During use, the shaking ball head 402 moves omnidirectionally within the central ball seat 207 of the top cover. The top arm of the mixing liquid shaking and stirring rod 401 is sleeved within the inclined grip tube 205. The central ball seat 207 of the top cover and the top cover drive motor 203 are coaxial. The top cover drive motor 203 drives the inclined grip tube 205 to rotate. Simultaneously, through the cooperation of the above structures, the bottom of the mixing liquid shaking and stirring assembly 400 achieves circular stirring within the ultrasonic mixing cylinder assembly 100 of the preparation liquid. In this way, the solution A and suspension B are thoroughly stirred and mixed. At the same time, a mixing liquid shaking and stirring support rod 403 and an ultrasonic generator 404 are fixedly installed at the bottom of the mixing liquid shaking and stirring rod 401. The combined action of the mixing stirring rod 403 and the ultrasonic generator 404 further ensures the mixing quality of solution A and suspension B. An aeration pipe 105 is fixedly installed at the bottom of the ultrasonic mixing cylinder 101, connected to an external high-pressure air supply tank. High-pressure air supply through the aeration pipe 105 causes the liquid at the bottom of the ultrasonic mixing cylinder 101 to tumble upwards, further ensuring thorough mixing of the solution inside. A top cover spray pipe 206 is fixedly installed inside the mixing top cover 201, connected to an external high-pressure liquid supply pump. This facilitates high-pressure rinsing when the device is idle. The mixing cylinder stirring shaft 50 on the pre-mixing cylinder assembly 500... A stirring support rod 510 for the preparatory liquid mixing cylinder is fixedly installed on the top of the preparatory liquid mixing cylinder 501. A pneumatic vent pipe 504 and a high-pressure flushing pipe 505 are fixedly installed at both ends of the inner top of the preparatory liquid mixing cylinder 501. The stirring support rod 510 is driven to rotate by the stirring motor 506, achieving stirring during the pre-preparation of solution A or suspension B. The pneumatic vent pipe 504 pressurizes the preparatory liquid mixing cylinder 501, allowing the solution in the preparatory liquid mixing cylinder 501 to smoothly flow into the ultrasonic mixing cylinder 101 for the preparation liquid. The high-pressure flushing pipe 505 provides high-pressure flushing when the preparatory liquid mixing cylinder 501 is idle. A rocker arm base assembly 300 is rotatably installed at the bottom of the ultrasonic mixing cylinder assembly 100 for the preparation liquid.At this time, the bottom of the main shaft 104 of the base arm is rotatably connected to the distal arm of the support arm 311 through a bearing seat. The ring-shaped central gear 307 meshes with the three driven gears 312 on the three rotating main arm shafts 310. The three support arms 311 are located between the ultrasonic mixing cylinder 101 of the preparation liquid and the ring-shaped rocker arm base 301. The three support arms 311 are distributed in parallel. The bottom of the rotating main arm shaft 310 is rotatably connected to the rocker arm side platform 302 through a bearing seat. The ring-shaped central gear 307 is driven to rotate by the drive motor 306. Simultaneously, through the cooperation of the above structures, the ultrasonic mixing cylinder assembly 100 of the preparation liquid and the mixing cylinder assembly 500 of the preparative liquid are subjected to circular shaking on the rocker arm base assembly 300. This external shaking drive further achieves mixing of the liquids inside the ultrasonic mixing cylinder assembly 100 and the preparative liquid mixing cylinder assembly 500. Through this external shaking mixing and internal mixing, thorough mixing of the two-stage preparation solutions is achieved, ensuring sufficient and efficient mixing during the preparation of porous starch ester loaded with naringin.

[0119] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An apparatus for preparing octenyl succinic acid-modified porous starch ester-supported naringin complex, characterized in that: The system includes a preparation liquid ultrasonic mixing cylinder assembly (100), with a mixing top cover assembly (200) on top and a mixing liquid shaking and stirring assembly (400) on the mixing top cover assembly (200); a rocker arm base assembly (300) is rotatably mounted on the bottom of the preparation liquid ultrasonic mixing cylinder assembly (100); and preparatory liquid mixing cylinder assemblies (500) are fixedly mounted on both sides of the top of the preparation liquid ultrasonic mixing cylinder assembly (100); the preparation liquid ultrasonic... The mixing cylinder assembly (100) includes an ultrasonic mixing cylinder (101) for preparing the liquid. Multiple mixing cylinder bottom arm platforms (102) are fixedly installed on the ultrasonic mixing cylinder (101) by reinforcing ribs (103). A bottom arm platform main shaft (104) is fixedly installed on the mixing cylinder bottom arm platform (102). An aeration pipe (105) is fixedly installed at the bottom end inside the ultrasonic mixing cylinder (101). A liquid pump (106) is fixedly installed on the outer side of the bottom of the ultrasonic mixing cylinder (101). The rocker arm base assembly (300) includes an annular rocker arm base frame (301), with multiple rocker arm side platforms (302) symmetrically distributed and fixedly arranged on the edge of the annular rocker arm base frame (301), and a frame support leg (303) fixedly arranged at the bottom of the rocker arm side platform (302); a first fixed plate (305) is fixedly arranged inside the annular rocker arm base frame (301) through a first support rod (304), a drive motor (306) is fixedly arranged at the bottom of the first fixed plate (305), a second fixed plate (308) is fixedly arranged on the shaft of the drive motor (306), and an annular central gear (307) is fixedly arranged outside the second fixed plate (308) through a second support rod (309); The top of the rocker arm side platform (302) is rotatably mounted with a rotating main arm shaft (310) via a bearing seat. A support arm (311) is fixedly mounted on the top of the rotating main arm shaft (310). The far end of the support arm (311) is rotatably connected to the bottom of the bottom arm platform main shaft (104) via a bearing seat. A driven gear (312) is fixedly mounted in the middle of the rotating main arm shaft (310). The driven gear (312) meshes with the ring-shaped central gear (307).

2. The apparatus for preparing octenyl succinic acid-modified porous starch ester-supported naringin complex according to claim 1, characterized in that: The hybrid top cover assembly (200) includes a hybrid top cover (201), a top cover spray pipe (206) is fixedly installed inside the hybrid top cover (201), a top cover frame plate (202), a vent pipe (208) and a top cover center ball seat (207) are fixedly installed on the top of the hybrid top cover (201), a top cover drive motor (203) is fixedly installed on the top of the top cover frame plate (202), and an inclined grip pipe (205) is fixedly installed on the shaft of the top cover drive motor (203) through a support side plate (204).

3. The apparatus for preparing octenyl succinic acid-modified porous starch ester-supported naringin complex according to claim 2, characterized in that: The mixing liquid shaking and stirring assembly (400) includes a mixing liquid shaking and stirring rod (401), a shaking ball head (402) is fixedly provided on the top of the mixing liquid shaking and stirring rod (401), and a mixing liquid shaking and stirring support rod (403) and an ultrasonic generator (404) are fixedly provided on the bottom of the mixing liquid shaking and stirring rod (401); the top arm of the mixing liquid shaking and stirring rod (401) is sleeved in the inclined grip tube (205).

4. The apparatus for preparing octenyl succinic acid-modified porous starch ester-supported naringin complex according to claim 1, characterized in that: The preparatory liquid mixing cylinder assembly (500) includes a preparatory liquid mixing cylinder (501). A feeding hopper (507) and a preparatory liquid outlet bottom pipe (502) are fixedly installed at the top and bottom of the preparatory liquid mixing cylinder (501), respectively. A snap-fit ​​cap (508) and an electromagnetic metering valve (503) are respectively installed on the feeding hopper (507) and the preparatory liquid outlet bottom pipe (502). A preparatory liquid mixing cylinder stirring motor (506) is fixedly installed on one side of the top of the preparatory liquid mixing cylinder (501). A preparatory liquid mixing cylinder stirring support rod (510) is fixedly installed on the preparatory liquid mixing cylinder stirring shaft (509) of the preparatory liquid mixing cylinder stirring motor (506). A pneumatic vent pipe (504) and a preparatory liquid mixing cylinder high-pressure flushing pipe (505) are fixedly installed at both ends of the inner side of the top of the preparatory liquid mixing cylinder (501).

5. The apparatus for preparing octenyl succinic acid-modified porous starch ester-supported naringin complex according to claim 3, characterized in that: The rocking ball head (402) moves in all directions within the central ball seat platform (207) of the top cover; the central ball seat platform (207) of the top cover and the drive motor (203) of the top cover are coaxial.

6. The apparatus for preparing octenyl succinic acid-modified porous starch ester-supported naringin complex according to claim 1, characterized in that: Multiple support arms (311) are located between the ultrasonic mixing cylinder (101) of the preparation liquid and the ring-shaped rocker arm base frame (301), and the multiple support arms (311) are distributed in a parallel structure.

7. The apparatus for preparing octenyl succinic acid-modified porous starch ester-supported naringin complex according to claim 1, characterized in that: The two sets of preparative liquid mixing cylinder assemblies (500) at the top of the ultrasonic mixing cylinder assembly (100) are used as naringin dissolving cylinder and octenyl succinic acid porous starch mixing cylinder, respectively. The method for preparing the octenyl succinic acid modified porous starch ester loaded with naringin complex Includes the following steps, (S01) Dissolve an appropriate amount of naringin in solvent I to prepare solution A; (S02) Take an appropriate amount of octenyl succinic acid porous starch and add it to solvent II. After dispersing and mixing, suspension B is prepared. (S03) The solution A in step (S01) and the suspension B in step (S02) are placed in an ultrasonic mixing device for ultrasonic mixing, and then centrifuged and dried to obtain the octenyl succinic acid modified porous starch ester loaded with naringin complex.

8. The apparatus for preparing octenyl succinic acid-modified porous starch ester-supported naringin complex according to claim 7, characterized in that: The ultrasonic intensity is 10W to 300W; the ultrasonic duration is 15min to 30min.

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