Method for improving quality of air-cured tobacco and application

Abstract

The invention discloses a method for improving the quality of air-cured tobacco and application. The method comprises the following steps: sequentially carrying out vacuum moisture regaining, leaf moistening and primary storage on tobacco leaves to obtain pretreated tobacco leaves; mixing the pretreated tobacco leaves with the biological fermentation material liquid, and carrying out secondary storage to obtain fermented tobacco leaves; wherein the biological fermentation material liquid comprises a fermentation extracting solution of a bacterial strain; and redrying the fermented tobacco leaves to obtain finished tobacco leaves. According to the method, the peculiar smell of the air-cured tobacco can be effectively reduced, the compatibility of the low-quality air-cured tobacco leaves is remarkably improved, and the tobacco leaves treated through the method can be directly used as formula raw materials of tobacco shreds of flue-cured tobacco type cigarettes. According to the method, the process is simple, and the use value of the sun-cured tobacco can be improved without modifying equipment or adopting multi-process combination.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of tobacco processing, in particular to a method for improving the quality of air-cured tobacco and application thereof. BACKGROUND

[0002] Air-cured tobacco refers to tobacco dried and cured in the sun or ventilated environment after harvesting, including burley, sun-cured tobacco, sun-cured red tobacco, oriental tobacco and cigar tobacco. Air-cured tobacco has great quality difference, only a small part of domestic high-quality air-cured tobacco can be used as raw materials for cigar tobacco and mixed cigarettes, and enter the formula. Low-grade air-cured tobacco has a high nicotine and ammonia content and a low sugar content, which makes it have a distinct air-cured tobacco aroma, and also has a pungent taste and irritation, so it is difficult to use in cigarette formulas, and has a large inventory.

[0003] At present, in some studies, the unique aroma of air-cured tobacco is reduced by a threshing and redrying production line, biological enzyme feeding, inactivation and expanded tobacco line processing technology, but the process is complex. There is no method for directly using biological fermentation liquid on the threshing and redrying production line to improve the use value of air-cured tobacco from the perspective of reducing the unique aroma of air-cured tobacco.

[0004] Therefore, it is of great significance to develop a new method for improving the use value of air-cured tobacco. SUMMARY

[0005] Therefore, it is of great significance to develop a new method for improving the use value of air-cured tobacco.

[0006] The first aspect of the present application provides a method for improving the quality of air-cured tobacco, comprising the following steps:

[0007] vacuum conditioning, leaf conditioning and primary storage of the tobacco leaves in sequence to obtain pretreated tobacco leaves;

[0008] mixing the pretreated tobacco leaves with a biological fermentation liquid to perform secondary storage and obtain fermented tobacco leaves; wherein the biological fermentation liquid comprises a fermentation extract of a bacterial strain;

[0009] redrying the fermented tobacco leaves to obtain finished tobacco leaves.

[0010] In some embodiments, the bacterial strain comprises at least one of Bacillus and a fungus for both food and medicine.

[0011] In some embodiments, the Bacillus includes one or more of Bacillus kochii, and the fungus includes one or more of Cordyceps sinensis, Tremella aurantialba, Ganoderma lucidum, and Taiwanofungus camphoratus.

[0012] In some embodiments, the bio-fermentation liquid further includes at least one of an enzyme preparation and an aqueous solvent, wherein the enzyme preparation has an enzyme activity concentration of 300 U / mL to 500 U / mL.

[0013] In some embodiments, in the bio-fermentation liquid, the mass ratio of the fermentation extract of the strain, the enzyme preparation, and the aqueous solvent is (1-5):(0-1):(10-150).

[0014] In some embodiments, the enzyme preparation includes at least one of amylase, cellulase, alkaline protease, neutral protease, and saccharifying enzyme, and / or the aqueous solvent includes at least one of soft water, mineral water, and pure water.

[0015] In some embodiments, the steps of sequentially vacuum conditioning, conditioning, and primary storage of the tobacco leaves satisfy one or more of the following conditions:

[0016] The vacuum conditioning is performed for two cycles;

[0017] The conditioning center temperature of the vacuum conditioning is 50°C to 65°C, and the moisture of the tobacco leaves after conditioning is increased by 2.0% (w / w) to 8.0% (w / w);

[0018] The conditioning includes at least one conditioning, the outlet moisture of the conditioning is 15% (w / w) to 25% (w / w), and the outlet temperature is 40°C to 50°C;

[0019] The primary storage has a temperature of 15°C to 65°C and a time of 1 h to 4 h.

[0020] In some embodiments, the step of mixing the pretreated tobacco leaves with a bio-fermentation liquid and performing secondary storage satisfies one or more of the following conditions:

[0021] The mass ratio of the pretreated tobacco leaves to the bio-fermentation liquid is 100:(1-10);

[0022] The secondary storage has a temperature of 15°C to 45°C and a time of 10 h to 18 h.

[0023] In some embodiments, the re-drying zone temperature in the re-drying process is 50-80℃, the cooling zone temperature is 35-45℃, and the moisture content of the outlet material is 13%(w / w)-17%(w / w).

[0024] In some embodiments, the method for preparing the fermentation extract of the strain comprises the following steps:

[0025] The strain is cultured until the absorbance OD 600nm of the fermentation broth is 0.8-0.9, and the fermentation is terminated to obtain the fermentation broth.

[0026] The fermentation broth is mixed with an aqueous ethanol solution, shaken, and filtered to obtain the fermentation extract of the strain.

[0027] In some embodiments, the method for preparing the fermentation extract of the strain satisfies one or more of the following conditions:

[0028] In the step of mixing the fermentation broth with the aqueous ethanol solution, the mass ratio of the fermentation broth to the aqueous ethanol solution is 1:(1-5).

[0029] The concentration of ethanol in the aqueous ethanol solution is 50%(v / v)-90%(v / v).

[0030] The shaking time is 2-4h.

[0031] The filtering is performed using 8-10 layers of gauze.

[0032] In some embodiments, the tobacco leaves include at least one of sun-cured tobacco leaves, Oriental tobacco leaves, Burley tobacco leaves, and cigar tobacco leaves; and the form of the tobacco leaves includes at least one of cut tobacco and sheet tobacco.

[0033] The second aspect of the present application provides a method for preparing a tobacco product, which comprises the method for improving the quality of air-cured tobacco leaves according to the first aspect of the present application.

[0034] The tobacco product is prepared using the treated tobacco leaves.

[0035] The third aspect of the present application provides a flue-cured tobacco cigarette, which comprises cut tobacco, and the cut tobacco comprises tobacco leaves treated by the method for improving the quality of air-cured tobacco leaves according to the first aspect of the present application.

[0036] The method for improving the quality of sun-cured tobacco provided in the foregoing effectively reduces the unique aroma of sun-cured tobacco by means of a threshing and redrying production line and a biological fermentation material treatment process, significantly improves the compatibility of low-quality sun-cured tobacco, and the sun-cured tobacco treated by the method can be directly used as a formula raw material of a flue-cured tobacco type cigarette. The method is simple in process and can improve the use value of sun-cured tobacco without modification of equipment or use of multiple processes. DETAILED DESCRIPTION

[0037] For the purpose of facilitating the understanding of the present application, the present application is described more fully below. However, the present application can be realized in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present application more thorough and comprehensive.

[0038] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the present application belongs. The terminology used in the description of the present application herein only for the purpose of describing specific embodiments and is not intended to limit the present application. The term "and / or" used herein includes any and all combinations of one or more relevant listed items.

[0039] The term "and / or", "or / and", "and / or" used herein includes any and all combinations of one or more relevant listed items, and also includes any and all combinations of relevant listed items, including any two relevant listed items, any more relevant listed items, or all relevant listed items.

[0040] In the present application, "multiple", "various", "multiple times", "multiple", etc. are used without specific limitation, which means more than two or equal to two in quantity. For example, "one or more" means one or more than two.

[0041] As used herein, "combinations thereof", "any combination thereof", "any combination thereof", etc. include all suitable combinations of any two or more listed items.

[0042] As used herein, "suitable combinations", "suitable ways", "any suitable way", etc. are "suitable" as long as the technical solutions of the present application can be implemented, the technical problems of the present application can be solved, and the expected technical effects of the present application can be achieved.

[0043] As used herein, "preferably", "better", "better", "preferably" only describes the implementation mode or embodiment with better effect, and it should be understood that it does not constitute a limitation on the protection scope of the present application.

[0044] In the present application, "further", "even further", "in particular" and the like are used for descriptive purposes only and should not be interpreted as limiting the scope of the present application.

[0045] In the present application, "optionally", "optional" and "may" mean that it is optional, i.e. selected from "yes" or "no". If there are multiple "optionally" in a technical solution, each "optionally" is independent unless otherwise specified, and there is no contradiction or mutual restriction.

[0046] In the present application, the terms "first", "second", and the like in "first aspect", "second aspect" and the like are only used for descriptive purposes and should not be interpreted as indicating or implying relative importance or quantity, nor should it be interpreted as implicitly indicating the importance or quantity of the technical features indicated. Moreover, "first", "second" and the like only serve the purpose of non-exhaustive enumeration description, and should be understood as not constituting a closed limitation on the quantity.

[0047] In the present application, the technical features described in an open manner include both the closed technical solution consisting of the listed features and the open technical solution including the listed features.

[0048] In the present application, with respect to the numerical interval (i.e. numerical range), if not otherwise specified, the optional numerical distribution within the above numerical interval is considered to be continuous, and includes the two numerical endpoints (i.e. the minimum value and the maximum value) of the numerical range, as well as every numerical value between the two numerical endpoints. If not otherwise specified, when the numerical interval only points to the integers within the numerical interval, including the two endpoint integers of the numerical range and every integer between the two endpoints, in this document, it is equivalent to directly listing each integer, for example, t is an integer selected from 1~10, which means that t is any integer selected from the group consisting of 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10. In addition, when multiple ranges are provided to describe characteristics or properties, these ranges can be combined. In other words, unless otherwise specified, the ranges disclosed in this document should be understood to include any and all sub-ranges incorporated therein.

[0049] In the present application, the temperature parameter, if not otherwise specified, allows for constant temperature treatment, and also allows for variation within a certain temperature range. It should be understood that the constant temperature treatment allows the temperature to fluctuate within the accuracy range controlled by the instrument. It is allowed to fluctuate within the range of, for example, ±5℃, ±4℃, ±3℃, ±2℃, ±1℃.

[0050] In the present application, %(w / w) and wt% both represent weight percentage, %(v / v) represents volume percentage, and %(w / v) represents mass volume percentage.

[0051] All the documents mentioned in the present application are incorporated by reference into the present application as if each document were individually incorporated. The cited documents are incorporated by reference in their entirety, for all purposes, unless and to the extent that the present application contradicts or otherwise explicitly rejects the cited document. When the present application refers to the cited documents, the definitions of the relevant technical features, terms, names, phrases, etc. in the cited documents are also incorporated by reference. When the present application refers to the cited documents, the examples, preferred modes of the relevant technical features cited are also incorporated by reference into the present application, subject to the ability to implement the present application. It should be understood that when the cited content conflicts with the description in the present application, the present application is given priority or is modified adaptively according to the description in the present application.

[0052] At present, in some studies, the specific aroma of air-cured tobacco is reduced by processing technology of leaf threshing and redrying production line, biological enzyme feeding, inactivation and expanded tobacco line, but the process is complex. There is no method of directly using biological fermentation broth to improve its value on the leaf threshing and redrying production line from the aspect of reducing the specific aroma of air-cured tobacco.

[0053] Based on this, the embodiments of the present application at least provide a method for improving the quality of air-cured tobacco and application.

[0054] In the present application, the term "vacuum conditioning" refers to using a vacuum environment combined with moisture (or steam) treatment to make the tobacco quickly and uniformly absorb moisture, while improving the toughness, elasticity and bulkiness of the tobacco.

[0055] In the present application, the term "leaf conditioning" refers to the process of moistening and warming the tobacco in the leaf threshing and redrying or tobacco processing production line, so that the tobacco is soft and loose, and the processing resistance is improved, and the breakage loss in the subsequent process is reduced.

[0056] In the present application, the term "storage" refers to the process of temporarily storing the tobacco that has been subjected to leaf conditioning under specific environmental conditions (temperature, humidity, time) to achieve further moisture balance, physical property stability and preliminary coordination of intrinsic quality of the tobacco.

[0057] In the present application, the term "redrying" refers to the process of adjusting the moisture content of the primary cured tobacco to the stable range required for long-term storage by precisely controlling the temperature and moisture.

[0058] In the first aspect of the present application, a method for improving the quality of air-cured tobacco is provided, which comprises the following steps:

[0059] S100: sequentially subjecting the tobacco to vacuum conditioning, leaf conditioning and primary storage to obtain pretreated tobacco;

[0060] S200: mixing the pretreated tobacco with a biological fermentation broth and performing secondary storage to obtain fermented tobacco; wherein the biological fermentation broth comprises a fermentation extract of a bacterial strain;

[0061] S300: curing the fermented tobacco leaves to obtain finished tobacco leaves.

[0062] In some embodiments, the tobacco leaves comprise at least one of sun-cured tobacco leaves, Oriental tobacco leaves, Burley tobacco leaves, and cigar tobacco leaves.

[0063] In some embodiments, the form of the tobacco leaves comprises at least one of cut tobacco and sheet tobacco.

[0064] In the present application, the term "cut tobacco" refers to a bundle of tobacco leaves of the same grade and quantity.

[0065] In the present application, the term "sheet tobacco" refers to a leaf part separated from the tobacco leaves after a threshing and stalk-removing process.

[0066] The method provided by the embodiments of the present application directly applies the bio-fermented liquid, and reduces the unique aroma of sun-cured tobacco by means of a threshing and curing production line, thereby improving the compatibility of the tobacco leaves. The treated tobacco leaves can be used as a formula of flue-cured tobacco cigarette tobacco.

[0067] In some embodiments, in step S100, the vacuum conditioning adopts two cycles.

[0068] In some embodiments, in step S100, the conditioning center temperature of the vacuum conditioning is 50-65°C, and the moisture of the tobacco leaves after conditioning is increased by 2.0% (w / w)-8.0% (w / w). Without limitation, the conditioning center temperature can be, but is not limited to, 50°C, 55°C, 60°C, 65°C, or a value or range between any two of the above values; the moisture of the tobacco leaves after conditioning is increased by 2.0% (w / w), 3.0% (w / w), 4.0% (w / w), 5.0% (w / w), 6.0% (w / w), 7.0% (w / w), 8.0% (w / w), or a value or range between any two of the above values.

[0069] In some embodiments, in step S100, the leaf moistening comprises at least one leaf moistening, and the outlet moisture of the leaf moistening is 15%-25% (w / w), and the outlet temperature is 40-65°C. Without limitation, the outlet moisture of the leaf moistening can be 15% (w / w), 17% (w / w), 19% (w / w), 21% (w / w), 23% (w / w), 25% (w / w), or a value or range between any two of the above values; the outlet temperature can be 40°C, 45°C, 50°C, 55°C, 60°C, 65°C, or a value or range between any two of the above values.

[0070] In some embodiments, in step S100, when the tobacco leaf form is a tobacco strand, the leaf conditioning comprises twice leaf conditioning, the first leaf conditioning has an outlet moisture content of 18% (w / w) to 22% (w / w) and an outlet temperature of 40°C to 50°C; and the second leaf conditioning has an outlet moisture content of 20% to 25% and an outlet temperature of 45°C to 65°C.

[0071] In some embodiments, in step S100, when the tobacco leaf form is a tobacco sheet, the leaf conditioning comprises once leaf conditioning, the leaf conditioning has an outlet moisture content of 15% (w / w) to 25% (w / w) and an outlet temperature of 55°C to 65°C.

[0072] In some embodiments, in step S100, the first sheet storage has a temperature of 15°C to 65°C and a time of 1h to 4h. Without limitation, the first sheet storage can have a temperature of, but not limited to, 15°C, 25°C, 35°C, 45°C, 55°C, 65°C, or a value or range between any two of the above values; and a time of, but not limited to, 1h, 2h, 3h, 4h, or a value or range between any two of the above values.

[0073] In some embodiments, in step S200, the strain comprises at least one of Bacillus and a medicinal and edible fungus. Specifically, the Bacillus comprises Bacillus kochii, and the medicinal and edible fungus comprises one or more of Cordyceps sinensis, Tremella aurantialba, Ganoderma lucidum, and Taiwanofungus camphoratus.

[0074] In some embodiments, the Bacillus kochii comprises Bacillus kochii with a preservation number of GDMCC No. 61029.

[0075] In some embodiments, the Cordyceps sinensis comprises Cordyceps sinensis with a preservation number of CGMCC No. 22451.

[0076] In some embodiments, the Tremella aurantialba comprises Tremella aurantialba with a preservation number of CGMCC No. 23831.

[0077] In some embodiments, the Ganoderma lucidum includes a Ganoderma lucidum with a preservation number of CGMCC No. 41576. The taxonomic name of the Ganoderma lucidum is Ganoderma lucidum, which has been preserved in the China General Microbiological Culture Collection Center, located at No. 1, Beichen West Road, Haidian District, Beijing, on October 18, 2024, with a preservation number of CGMCC No. 41576. The strain was received and registered by the preservation center on October 18, 2024, and was detected by the preservation center on October 18, 2024, as a viable strain.

[0078] In some embodiments, the Taiwanofungus camphoratus includes a Taiwanofungus camphoratus with a preservation number of CGMCC No. 41577. The taxonomic name of the Taiwanofungus camphoratus is Taiwanofungus camphoratus, which has been preserved in the China General Microbiological Culture Collection Center, located at No. 1, Beichen West Road, Haidian District, Beijing, on October 18, 2024, with a preservation number of CGMCC No. 41577. The strain was received and registered by the preservation center on October 18, 2024, and was detected by the preservation center on October 18, 2024, as a viable strain.

[0079] In some embodiments, the strains from the above-mentioned biological preservation sources or their derived strains can be used. The derived strains can include genetically engineered new strains. It can be understood that the extract obtained by fermentation of the derived strains after modification still has the function of reducing the aroma of tobacco air-cured tobacco.

[0080] In some embodiments, in step S200, the biological fermentation feed liquid further includes at least one of an enzyme preparation and an aqueous solvent. Illustratively, the enzyme preparation includes at least one of amylase, cellulase, alkaline protease, neutral protease, and saccharifying enzyme, and / or the aqueous solvent includes at least one of soft water, mineral water, and pure water.

[0081] In some embodiments, the active concentration of the enzyme in the enzyme preparation is 300 U / mL to 500 U / mL. Non-limitingly, the active concentration of the enzyme in the enzyme preparation can be, but is not limited to, 300 U / mL, 400 U / mL, 500 U / mL, or a value or range between any two of the above values.

[0082] In some embodiments, the mass ratio of the fermentation extract of the strain, the enzyme preparation, and the aqueous solvent in the bio-fermentation feedstock is (1-5):(0-1):(10-150). Without limitation, the mass ratio of the fermentation extract of the strain, the enzyme preparation, and the aqueous solvent can be, but is not limited to, 1:0:10, 1:0:50, 1:0:100, 1:0:150, 3:0:10, 3:0:50, 3:0:100, 3:0:150, 5:0:10, 5:0:50, 5:0:100, 5:0:150, 1:1:10, 1:1:50, 1:1:100, 1:1:150, 3:1:10, 3:1:50, 3:1:100, 3:1:150, 5:1:10, 5:1:50, 5:1:100, 5:1:150, or a ratio or range between any two of the above ratios.

[0083] In some embodiments, in step S200, the mass ratio of the pretreated tobacco leaf to the bio-fermentation feedstock is 100:(1-10). Without limitation, the mass ratio of the pretreated tobacco leaf to the bio-fermentation feedstock can be, but is not limited to, 100:1, 100:2, 100:3, 100:4, 100:5, 100:6, 100:7, 100:8, 100:9, 100:10, or a ratio or range between any two of the above ratios.

[0084] In some embodiments, in step S200, the temperature of the secondary storage of the tobacco leaf is 15-45°C for 10-18 hours. Without limitation, the temperature of the secondary storage of the tobacco leaf can be, but is not limited to, 15°C, 20°C, 25°C, 30°C, 35°C, 40°C, 45°C, or a value or range between any two of the above values.

[0085] In some embodiments, in step S300, the temperature of the drying zone in the redrying is 50-80°C, the temperature of the cooling zone is 35-45°C, and the moisture content of the outlet material is 13% (w / w)-17% (w / w). Without limitation, the temperature of the drying zone in the redrying can be, but is not limited to, 50°C, 60°C, 70°C, 80°C, or a value or range between any two of the above values; the temperature of the cooling zone can be, but is not limited to, 35°C, 40°C, 45°C, or a value or range between any two of the above values; and the moisture content of the outlet material can be, but is not limited to, 13% (w / w), 14% (w / w), 15% (w / w), 16% (w / w), 17% (w / w), or a value or range between any two of the above values.

[0086] In some embodiments, in step S300, after the redrying of the tobacco leaf, the tobacco leaf is stored in a box, the moisture content of the tobacco leaf in the box is 12%-15%, and the storage time is less than 90 days.

[0087] In some embodiments, the method for preparing the fermentation extract of the strain comprises the following steps:

[0088] S100': culturing the strain until the absorbance OD 600nm of the fermentation broth is 0.8-0.9, and terminating the fermentation to obtain the fermentation broth;

[0089] S200': mixing the fermentation broth with an aqueous ethanol solution, oscillating and filtering to obtain the fermentation extract of the strain.

[0090] In some embodiments, in step S200', the mass ratio of the fermentation broth to the aqueous ethanol solution is 1:(1-5). Non-limitingly, the mass ratio of the fermentation broth to the aqueous ethanol solution can be, but is not limited to, 1:1, 1:2, 1:3, 1:4, 1:5, or a ratio or range between any two of the above.

[0091] In some embodiments, in step S200', the concentration of ethanol in the aqueous ethanol solution is 50%(v / v)-90%(v / v). Non-limitingly, the concentration of ethanol in the aqueous ethanol solution can be, but is not limited to, 50%(v / v), 60%(v / v), 70%(v / v), 80%(v / v), 90%(v / v), or a value or range between any two of the above.

[0092] In some embodiments, in step S200', the oscillation time is 2h-4h. Non-limitingly, the oscillation time can be, but is not limited to, 2h, 3h, 4h, or a value or range between any two of the above.

[0093] In some embodiments, in step S200', the filtering is performed using 8, 9, or 10 layers of gauze.

[0094] In a second aspect of the present application, a method for preparing a tobacco product is provided, comprising treating the tobacco leaves using the method for improving the quality of sun-cured tobacco leaves described above;

[0095] The treated tobacco leaves are used to prepare the tobacco product.

[0096] In some embodiments, the tobacco product comprises at least one of a cigar, a hookah, a pipe, a self-rolled tobacco, and snuff.

[0097] In a third aspect of the present application, a flue-cured tobacco cigarette is provided, comprising tobacco shreds, wherein the tobacco shreds comprise treated tobacco leaves prepared using the method for improving the quality of sun-cured tobacco leaves described above.

[0098] In some embodiments, the treated tobacco leaves are used as a formula of flue-cured tobacco cigarette shreds.

[0099] Some embodiments are provided below.

[0100] The embodiments of the present application will be described in detail below with reference to the examples. It should be understood that these examples are only used to illustrate the present application and not used to limit the scope of the present application. The experimental methods in the following examples without specified conditions, the priority is given to the guidance given in the present application, but also can be in accordance with the experimental manual or conventional conditions in the art, but also in accordance with the conditions suggested by the manufacturer, or reference to the known experimental methods in the art.

[0101] In the following examples, the amount of raw material components involved in the parameter, such as no special mention, there may be a slight deviation in the range of weighing accuracy. Involving temperature and time parameters, the acceptable deviation caused by the accuracy of the instrument test or operation accuracy.

[0102] In the following examples, Bacillus kochii, Cordyceps sinensis, Tremella aurantialba, Ganoderma lucidum, Taiwanofungus camphoratus were provided by Sichuan Tobacco Industry Co., Ltd. Technical Center, the preservation number of Bacillus kochii was GDMCC No. 61029. The preservation number of Cordyceps sinensis was CGMCC No. 22451, the preservation number of Tremella aurantialba was CGMCC No. 23831, the preservation number of Ganoderma lucidum was CGMCC No. 41576, and the preservation number of Taiwanofungus camphoratus was CGMCC No. 41577.

[0103] The enzyme preparation used was commercially available, including amylase (Beijing Aoboxin Biotechnology Co., Ltd., item number 01-067), cellulase (Shanghai Yuanye Biotechnology Co., Ltd., item number S10041-100g), alkaline protease (Beijing Aoboxin Biotechnology Co., Ltd., item number 01-132), neutral protease (Beijing Aoboxin Biotechnology Co., Ltd., item number 01-080), and saccharifying enzyme (Beijing Aoboxin Biotechnology Co., Ltd., item number 01-072).

[0104] In the following examples, the activity of cellulase was defined as follows: 1 g of enzyme powder (or 1 mL of enzyme solution) hydrolyzed carboxymethyl cellulose sodium substrate at 50°C and pH 4.8 for 1 h, producing an amount of reducing sugar equivalent to 1 mg of glucose, which was 1 unit of enzyme activity, expressed as u / g (or u / mL).

[0105] In the following examples, the components of LB medium included tryptone 10 g, yeast extract 5 g, sodium chloride 10 g; the components of potato glucose water medium included potato infusion powder 6 g, glucose 20 g.

[0106] In the following examples, cigar leaves are provided by Sichuan China Tobacco Industry Co., Ltd. Technical Center.

[0107] Example 1

[0108] After Bacillus kochii is activated for 2 generations in LB medium, it is inoculated into LB medium containing 0.5% (w / v) tobacco leaves for fermentation. When the OD600nm of the fermentation broth is 0.9, the fermentation broth is added to 90% ethanol aqueous solution at a mass ratio of 1:1, and then shaken for 2 h. After 8 layers of gauze are filtered, a fermentation extract is obtained.

[0109] The cigar leaf tobacco is vacuum rehydrated, with a rehydration center temperature of 55-60℃, and the moisture content of the rehydrated tobacco is increased by 3%. The first rehydration outlet moisture content of the cigar leaf tobacco is set to 18%, and the outlet temperature is 45-50℃. The second rehydration outlet moisture content is set to 25%, and the outlet temperature is 45-60℃. After rehydration, the tobacco is first stored for 2 h at a temperature of 25-30℃. The Bacillus kochii fermentation broth is diluted with soft water at a ratio of 1:10 to obtain a fermentation broth. The fermentation broth and the tobacco are mixed at a ratio of 1:50, and then the tobacco is secondly stored for 12-14 h at a temperature of 25-35℃. After storage, the tobacco is dried in a re-drying zone at a temperature of 75-80℃, and then cooled at a temperature of 35-45℃. The moisture content of the outlet material is 15%, and the finished tobacco leaves are obtained.

[0110] Example 2

[0111] After the Ophiocordyceps sinensis strain is activated for 2 generations in potato glucose water medium, it is inoculated into potato glucose water medium for fermentation. When the OD600nm of the fermentation broth is 0.8, the fermentation broth is added to 75% ethanol aqueous solution at a mass ratio of 1:1, and then shaken for 4 h. After 8 layers of gauze are filtered, a fermentation extract is obtained.

[0112] The cigar tobacco strips are vacuum conditioned, the conditioning center temperature is 55-60℃, and the moisture of the tobacco strips increases by 3% after conditioning. The outlet moisture of the first conditioning of the cigar tobacco strips is set to 15%, and the outlet temperature is 45-50℃. The outlet moisture of the second conditioning of the cigar tobacco strips is set to 25%, and the outlet temperature is 45-60℃. After conditioning, the tobacco strips are first stored for 2 h at a temperature of 25-30℃. The Cordyceps sinensis bio-fermentation broth is diluted with soft water at a ratio of 1:15 to obtain a bio-fermentation broth, and the tobacco strips are second stored after adding the bio-fermentation broth at a ratio of 1:100. The storage temperature is 25-35℃, and the storage time is 12-14 h. After storage, the tobacco strips pass through the re-drying area of the re-drying machine, the temperature is set to 75-80℃, the cooling area temperature is 35-45℃, and the outlet moisture of the material is 13%, thereby obtaining the finished tobacco leaves.

[0113] Example 3

[0114] The Auricularia auricula strain is activated in a potato glucose aqueous medium for 2 generations, inoculated into a potato glucose aqueous medium for fermentation, and when the OD600nm of the fermentation broth is 0.8, 75% ethanol aqueous solution is added to the fermentation broth at a mass ratio of 1:1, oscillation extraction is performed for 4 h, 8 layers of gauze are used for filtration, and fermentation extraction liquid is obtained.

[0115] The cigar tobacco strips are vacuum conditioned, the conditioning center temperature is 55-60℃, and the moisture of the tobacco strips increases by 3% after conditioning. The outlet moisture of the first conditioning of the cigar tobacco strips is set to 15%, and the outlet temperature is 45-50℃. The outlet moisture of the second conditioning of the cigar tobacco strips is set to 25%, and the outlet temperature is 45-60℃. After conditioning, the tobacco strips are first stored for 2 h at a temperature of 25-30℃. The Cordyceps sinensis bio-fermentation broth is diluted with soft water at a ratio of 1:15 to obtain a bio-fermentation broth, and the tobacco strips are second stored after adding the bio-fermentation broth at a ratio of 1:100. The storage temperature is 25-35℃, and the storage time is 12-14 h. After storage, the tobacco strips pass through the re-drying area of the re-drying machine, the temperature is set to 75-80℃, the cooling area temperature is 35-45℃, and the outlet moisture of the material is 13%, thereby obtaining the finished tobacco leaves.

[0116] Example 4

[0117] The cigar tobacco leaves are vacuum conditioned, the conditioning center temperature is 50-55℃, and the moisture of the leaves increases by 3% after conditioning. The outlet moisture of the first conditioning of the leaves is set to 18%, and the outlet temperature is 40-45℃. The outlet moisture of the second conditioning of the leaves is set to 25%, and the outlet temperature is 45-55℃. After conditioning, the leaves are first stored for 4 h at a temperature of 20-25℃. The cellulase is configured to 400 U / mL, and the biological fermentation liquid is prepared by mixing the Tremella aurantialba and the cellulase at a ratio of 10:1, and then diluting the liquid with soft water at a ratio of 1:20. The leaves are second stored for 12-14 h at a temperature of 25-30℃ after adding the biological fermentation liquid to the leaves at a ratio of 1:100. After storage, the leaves are dried in the drying area of the re-drying machine, the temperature is set to 75-80℃, the temperature of the cooling area is 35-45℃, and the outlet moisture of the leaves is 15%, and the finished product is obtained.

[0118] Example 5

[0119] The cigar tobacco leaves are vacuum conditioned, the conditioning center temperature is 55-60℃, and the moisture of the leaves increases by 3% after conditioning. The outlet moisture of the first conditioning of the leaves is set to 23%, and the outlet temperature is 50-55℃. After conditioning, the leaves are first stored for 2 h at a temperature of 25-30℃. The biological fermentation liquid is prepared by mixing the Tremella aurantialba, Cordyceps sinensis and cellulase at a ratio of 20:20:1, and then diluting the liquid with soft water at a ratio of 1:20. The leaves are second stored for 12-14 h at a temperature of 25-30℃ after adding the biological fermentation liquid to the leaves at a ratio of 1:100. After storage, the leaves are dried in the drying area of the re-drying machine, the temperature is set to 75-80℃, the temperature of the cooling area is 35-45℃, and the outlet moisture of the leaves is 15%, and the finished product is obtained.

[0120] Comparative Example 1

[0121] The cigar tobacco is vacuum rehydrated, the rehydration center temperature is 55-60 DEG C, and the moisture of the tobacco after rehydration is increased by 3%. The moisture of the first rehydration outlet of the cigar tobacco is set to 18%, and the outlet temperature is 45-50 DEG C. The moisture of the second rehydration outlet is set to 25%, and the outlet temperature is 45-60 DEG C. After rehydration, the tobacco is first stored for 2 hours at a temperature of 25-30 DEG C. The cellulase is configured to 400 U / mL, and the enzyme solution and the tobacco are added in a mass ratio of 1:100 for second storage for 12-14 hours at a temperature of 25-35 DEG C. After storage, the tobacco is dried in the re-drying area, the temperature is set to 75-80 DEG C, the cooling area temperature is 35-45 DEG C, and the outlet material moisture is 13%, thereby obtaining the finished tobacco.

[0122] Test effect:

[0123] The finished tobacco obtained from examples 1-5 and comparative example 1 is taken, and the original tobacco without treatment is taken, and after the moisture is balanced, a single tobacco is prepared, and sensory evaluation is carried out, and the results are shown in table 1. The method provided by the application can reduce the airing smoke of the tobacco, and has the effects of reducing the smoke concentration and increasing the oral comfort.

[0124] Table 1

[0125]

[0126] The finished tobacco obtained from examples 1-5 and comparative example 1 is taken, and the original tobacco without treatment is taken, and after the moisture is balanced, a single tobacco is prepared, and sensory evaluation is carried out, and the results are shown in table 1. The method provided by the application can reduce the airing smoke of the tobacco, and has the effects of reducing the smoke concentration and increasing the oral comfort.

[0127] Table 2

[0128]

[0129] The technical features of the above-described embodiments can be combined in any manner. To make the description concise, all possible combinations of the technical features in the above-described embodiments are not described, but as long as the combinations of the technical features do not contradict, they should be considered within the scope of the present disclosure.

[0130] The above-described embodiments only express several implementation manners of the present application, and the description is more specific and detailed, but it should not be understood as a limitation on the scope of the patent. It should be noted that for ordinary skilled persons in the art, without departing from the concept of the present application, several modifications and improvements can be made, which are within the scope of the present application. Therefore, the protection scope of the patent of the present application should be subject to the appended claims, and the description can be used to explain the content of the claims.

Claims

1. A method of improving the quality of sun-cured tobacco, characterized by, It comprises the following steps: The tobacco leaves are sequentially vacuum conditioned, leaf moistened, and first stored for a period of time to obtain pretreated tobacco leaves; The pretreated tobacco leaves are mixed with a biological fermentation liquid to perform second storage for a period of time to obtain fermented tobacco leaves; wherein the biological fermentation liquid comprises a fermentation extract of a strain; The fermented tobacco leaves are redried to obtain finished tobacco leaves.

2. The method of improving quality of sun-cured tobacco according to claim 1, wherein, The strain comprises at least one of Bacillus and a fungus for both food and medicine; Optionally, the Bacillus comprises Bacillus kochii, and the fungus for both food and medicine comprises one or more of Cordyceps sinensis, Tremella aurantialba, Ganoderma lucidum, and Taiwanofungus camphoratus.

3. The method of improving quality of sun-cured tobacco according to claim 2, wherein, The biological fermentation liquid further comprises at least one of an enzyme preparation and an aqueous solvent, wherein the enzyme in the enzyme preparation has an activity concentration of 300 U / mL to 500 U / mL; Optionally, in the biological fermentation liquid, the mass ratio of the fermentation extract of the strain, the enzyme preparation, and the aqueous solvent is (1-5):(0-1):(10-150); Further optionally, the enzyme preparation comprises at least one of amylase, cellulase, alkaline protease, neutral protease, and saccharifying enzyme, and / or the aqueous solvent comprises at least one of soft water, mineral water, and pure water.

4. The method of improving quality of sun-cured tobacco according to claim 1, wherein, The steps of sequentially vacuum conditioning, leaf moistening, and first storing for a period of time of the tobacco leaves satisfy one or more of the following conditions: The vacuum conditioning adopts two cycles; The conditioning center temperature of the vacuum conditioning is 50°C to 65°C, and the moisture of the tobacco leaves after conditioning increases by 2.0% (w / w) to 8.0% (w / w); The leaf moistening comprises at least one time of leaf moistening, the outlet moisture of the leaf moistening is 15% (w / w) to 25% (w / w), and the outlet temperature is 40°C to 50°C; The temperature of the first storage for a period of time is 15°C to 65°C, and the time is 1h to 4h.

5. The method of improving quality of sun-cured tobacco according to claim 1, wherein, The step of mixing the pretreated tobacco leaves with a biological fermentation liquid to perform second storage for a period of time satisfies one or more of the following conditions: The mass ratio of the pretreated tobacco leaves to the biological fermentation liquid is 100:(1-10); The temperature of the second storage for a period of time is 15°C to 45°C, and the time is 10h to 18h.

6. The method of improving quality of sun-cured tobacco according to claim 1, wherein, In the redrying, the temperature of the redrying zone is 50°C to 80°C, the temperature of the cooling zone is 35°C to 45°C, and the outlet moisture of the material is 13% (w / w) to 17% (w / w).

7. The method of improving quality of the sun-cured tobacco according to any one of claims 1 to 6, wherein, The preparation method of the fermentation extract of the strain comprises the following steps: The strain was cultured until the absorbance OD of the fermentation broth was 0.8-0.9 600nm The fermentation was terminated when the absorbance OD of the fermentation broth was 0.8-0.9, and a fermentation broth was obtained. The fermentation liquid is mixed with an aqueous ethanol solution, shaken, and filtered to obtain the fermentation extract of the strain; Optionally, the preparation method of the fermentation extract of the strain satisfies one or more of the following conditions: In the step of mixing the fermentation liquid with the aqueous ethanol solution, the mass ratio of the fermentation liquid to the aqueous ethanol solution is 1:(1-5); The concentration of ethanol in the aqueous ethanol solution is 50% (v / v) to 90% (v / v); The oscillation time is 2-4 hours; The filtering is performed by using 8-10 layers of gauze.

8. The method of improving quality of the sun-cured tobacco according to any one of claims 1 to 7, wherein, The tobacco leaves include at least one of sun-cured tobacco leaves, aromatic tobacco leaves, burley tobacco leaves and cigar tobacco leaves. Optionally, the form of the tobacco leaves includes at least one of cut tobacco and sheet tobacco.

9. A method of making a tobacco product, characterized by, The tobacco leaves are treated by using the method for improving the quality of sun-cured tobacco leaves according to any one of claims 1-8. The tobacco product is prepared by using the treated tobacco leaves.

10. A flue-cured type cigarette, characterized by, The tobacco product includes tobacco shreds, and the tobacco shreds include the treated tobacco leaves by using the method for improving the quality of sun-cured tobacco leaves according to any one of claims 1-8.

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