Resveratrol composition with whitening and spot-fading effects as well as preparation method and application of resveratrol composition

Abstract

The invention belongs to the field of daily chemical products, and particularly relates to a resveratrol composition with whitening and spot-fading effects as well as a preparation method and application of the resveratrol composition. The composition is prepared from the following raw materials: resveratrol microcapsules, glabridin, flos magnoliae extract, jasminum grandiflorum extract, willow leaf extract and equisetum arvense leaf extract. The flos magnoliae extract and the jasminum grandiflorum extract can endow the composition with excellent whitening and freckle removing performance, the willow leaf extract and the equisetum arvense leaf extract can effectively enhance the anti-inflammatory performance of the composition, and the obtained composition has excellent performance and is widely applied to cosmetics.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of daily chemicals, and particularly relates to a resveratrol composition with the effects of whitening and lightening spots, and a preparation method and application thereof. BACKGROUND

[0002] Whitening and lightening spots is a kind of skin care product for improving skin color and reducing local pigment deposition, thereby achieving the goal of lightening overall skin color and lightening spots. The principle of action is mainly based on the intervention of the process of skin melanin production: on the one hand, the synthesis of melanin is reduced from the source by inhibiting the activity of tyrosinase; on the other hand, the formed melanin is prevented from transferring to the epidermal keratinocytes, and the shedding of the aged keratinocytes containing pigments is promoted, and the metabolism of the skin is accelerated. The realization of this effect depends on the multi-pathway synergistic effect of the complex active ingredients, which blocks the activation of the external stimulation of melanocytes, and accelerates the shedding of the cells containing pigments and the metabolism of the skin cells (especially the excessive melanocytes).

[0003] The invention patent CN119732882B discloses a whitening and freckle-removing active composition, a preparation method and application thereof in cosmetics. The whitening and freckle-removing active composition comprises RHODIOLA CRENULATA root extract, MORUS ALBA extract and hexapeptide-2.

[0004] The invention patent CN116919878B discloses an anti-aging, whitening, freckle-removing and moisturizing skin care water and a preparation process thereof. The preparation process of the anti-aging, whitening, freckle-removing and moisturizing skin care water comprises the following steps: supercritical CO2 extraction of oat-Achillea extract; ultrasonic-enzyme assisted reflux extraction and concentration; addition of oxidized resveratrol, salidroside and ellagic acid; addition of pure water for dissolution and addition of sodium alginate and glycerol; addition of p-anisic acid and aromatic alcohol and homogeneous mixing. The skin care water is prepared by first extracting white and, artemisia leaf, cortex moutan, oat and Achillea extract, and then adding oxidized resveratrol, salidroside, ellagic acid, sodium alginate, glycerol, p-anisic acid and aromatic alcohol.

[0005] The invention patent CN115969756B discloses an anti-allergic, whitening and freckle-removing composition and a preparation method thereof. The anti-allergic, whitening and freckle-removing composition is composed of the following components in mass fraction: 0.5-2% of jonquil flower extract, 0.5-2% of lamp tree extract, 0.5-2% of Vanda Miss Joaquim extract, 0.5-2% of safflower wormwood flower extract, 1-3% of VC ethyl ether, 0.5-5% of arbutin, 0.1-2% of 4-butyl resorcinol, 1-10% of nicotinamide, 3-15% of moisturizing agent, 3-15% of emollient, 0.5-2% of penetrating agent, and water in the remainder.

[0006] The preparation method of the whitening and freckle-removing emulsion composition comprises the following steps: sequentially adding butanediol, white resveratrol, and glycerol into a blender, heating to 50-52 DEG C, stirring and mixing for 15-25 min at a stirring speed of 350-450 r / min, then adding nicotinamide into the mixture, keeping the temperature and stirring speed, and continuing to stir and mix for 10-15 min, and standby.

[0007] The application patent CN111920699B discloses a whitening and freckle-removing composition, a whitening and freckle-removing soluble microneedle patch, and a preparation method thereof. The whitening and freckle-removing composition is composed of oxidized white resveratrol, white resveratrol, and small molecule substances, the small molecule substances are composed of the following components in parts by weight: 40-50 parts of alpha-arbutin, 40-50 parts of glutathione, and 1-3 parts of soybean isoflavone, and the mass ratio of the total mass of the oxidized white resveratrol and the white resveratrol to the mass of the small molecule substances is 1:1-10. The whitening and freckle-removing soluble microneedle patch is prepared from a viscous liquid composed of an inclusion liquid of the oxidized white resveratrol and the white resveratrol, small molecule substances, a microneedle skeleton material, and a first solvent; and the inclusion liquid of the oxidized white resveratrol and the white resveratrol is prepared from the oxidized white resveratrol, the white resveratrol, hydroxypropyl-beta cyclodextrin, and a second solvent.

[0008] However, for white resveratrol related cosmetic raw materials, further improving the stability of white resveratrol and enhancing the whitening and freckle-removing effect of related compositions are still technical problems to be solved. SUMMARY

[0009] In view of the above technical problems, the present application provides a white resveratrol composition with whitening and freckle-removing effect, and a preparation method and application thereof.

[0010] In one aspect, the present application provides a white resveratrol composition with whitening and freckle-removing effect, and the raw material composition comprises white resveratrol microcapsules, glabridin, magnolia biondii flower extract, jasminum grandiflorum extract, epilobium angustifolium leaf extract, and equisetum arvense leaf extract.

[0011] Further, the weight ratio of the magnolia biondii flower extract and the jasminum grandiflorum extract in the white resveratrol composition is 1:0.5-4.

[0012] Further, the preparation method of the resveratrol microcapsule in the resveratrol composition comprises: dissolving resveratrol and ethyl cellulose in an organic solvent to obtain an oil phase; dissolving polyvinyl alcohol in water to obtain an aqueous phase; dropping the oil phase into the aqueous phase, and shearing and emulsifying to obtain an emulsion; concentrating and drying the emulsion to obtain the resveratrol microcapsule.

[0013] Further, the organic solvent comprises dichloromethane.

[0014] Further, in the resveratrol composition, the raw material composition comprises, by weight: 0.05-0.5 parts of resveratrol microcapsule, 0.05-0.5 parts of glabridin, 0.1-1.5 parts of MAGNOLIA BIONDII flower extract, 0.1-1.5 parts of JASMINUM GRANDIFLORUM extract, 0.1-1.5 parts of EPILOBIUM ANGUSTIFOLIUM leaf extract, and 0.1-1.5 parts of EQUISETUM ARVENSE leaf extract.

[0015] Further, in the resveratrol composition, the MAGNOLIA BIONDII flower extract is obtained by heating extraction with an ethanol solution with a concentration of 30-50 wt%.

[0016] Further, the preparation method of the MAGNOLIA BIONDII flower extract comprises: beating the flower of MAGNOLIA BIONDII and a 30-50 wt% ethanol solution, heating to 40-60℃, stirring and extracting for 2-6 hours, filtering, concentrating, and drying to obtain.

[0017] Further, in the resveratrol composition, the JASMINUM GRANDIFLORUM extract is obtained by heating extraction with an ethanol solution with a concentration of 35-55 wt%.

[0018] Further, the preparation method of the JASMINUM GRANDIFLORUM extract comprises: beating JASMINUM GRANDIFLORUM and a 35-55 wt% ethanol solution, heating to 40-60℃, stirring and extracting for 2-6 hours, filtering, concentrating, and drying to obtain.

[0019] Further, in the resveratrol composition, the EPILOBIUM ANGUSTIFOLIUM leaf extract is obtained by heating extraction with a 70-90 wt% ethanol solution.

[0020] Further, the preparation method of the EPILOBIUM ANGUSTIFOLIUM leaf extract comprises: beating EPILOBIUM ANGUSTIFOLIUM leaf and a 70-90 wt% ethanol solution, heating to 40-60℃, stirring and extracting for 2-6 hours, filtering, concentrating, and drying to obtain.

[0021] Further, in the resveratrol composition, the EQUISETUM ARVENSE leaf extract is obtained by heating extraction with a 70-90 wt% ethanol solution.

[0022] Further, the preparation method of the extract of Equisetum arvense includes: beating the Equisetum arvense and 70-90wt% ethanol solution, stirring and extracting at 40-60℃ for 2-6 hours, filtering, concentrating and drying to obtain.

[0023] Further, the weight ratio of the extract of Forsythia suspensa and the extract of Equisetum arvense in the resveratrol composition is 1:0.3-1.2.

[0024] The application also provides a preparation method of the resveratrol composition, which includes mixing raw materials, packaging and sterilizing.

[0025] The application also provides an application of the resveratrol composition in preparing a whitening and freckle-removing or anti-inflammatory cosmetic product. The amount of the resveratrol composition in the cosmetic product is 0.01-50wt%.

[0026] The application has the following beneficial effects compared with the prior art: The raw materials of the resveratrol composition of the application include glycerin, resveratrol microcapsules, glabriden, extract of Forsythia suspensa, extract of Plumeria rubra and extract of Equisetum arvense. The extract of Forsythia suspensa and the extract of Plumeria rubra can endow the composition with excellent whitening and freckle-removing performance, and the extract of Forsythia suspensa and the extract of Equisetum arvense can effectively enhance the anti-inflammatory performance of the composition. The composition obtained by the application has excellent performance and is widely used in cosmetic products.

[0027] The extract of Forsythia suspensa and the extract of Plumeria rubra used in the application can effectively whiten and remove freckles, and the inhibition efficiency of melanin synthesis reaches 51.26% and 53.39%. When the two are used in combination, a positive correlation promoting effect is presented in the ratio range of 1:0.1-12. In particular, in the ratio range of 1:0.3-12, a synergistic effect is presented, and in the ratio range of 1:0.5-4, the whitening and freckle-removing effect is stronger.

[0028] The anti-inflammatory effect of the composition prepared by the application is excellent. The extract of Forsythia suspensa and the extract of Equisetum arvense can effectively endow the composition with excellent anti-inflammatory effect and can effectively soothe and repair the skin. The anti-inflammatory effect of the composition using the extract of Forsythia suspensa and the extract of Equisetum arvense is better than that of using one of them, and the two are combined in the system of the application to promote each other and have a synergistic effect.

[0029] The composition prepared by the application has excellent performance and has a wide application prospect in cosmetic products. BRIEF DESCRIPTION OF DRAWINGS

[0030] Figure 1 : Whitening and freckle-removing test of extract.

[0031] Figure 2 : Anti-inflammatory test of composition. DETAILED DESCRIPTION

[0032] The technical solutions of the present application are further illustrated by specific embodiments below. Those skilled in the art should understand that the embodiments are only used to help understand the present application and should not be regarded as specific limitations of the present application.

[0033] Magnolia biondii flower extract: Magnolia biondii flowers and 40wt% ethanol solution were beaten at a weight ratio of 1:15, heated to 55℃, extracted for 4 hours with stirring at 60rpm, filtered, concentrated to a filtrate weight of 15.4% and freeze-dried to a water content of 1.34wt% to obtain.

[0034] Jasminum grandiflorum extract: Jasminum grandiflorum and 42wt% ethanol solution were beaten at a weight ratio of 1:13, heated to 50℃, extracted for 4.5 hours with stirring at 60rpm, filtered, concentrated to a filtrate weight of 13.6% and freeze-dried to a water content of 1.75wt% to obtain.

[0035] Epilobium angustifolium leaf extract: Epilobium angustifolium leaves and 80wt% ethanol solution were beaten at a weight ratio of 1:15, heated to 50℃, extracted for 4.5 hours with stirring at 60rpm, filtered, concentrated to a filtrate weight of 15.2% and freeze-dried to a water content of 1.67wt% to obtain.

[0036] Equisetum arvense leaf extract: Equisetum arvense leaves and 85wt% ethanol solution were beaten at a weight ratio of 1:16, heated to 48℃, extracted for 5.2 hours with stirring at 60rpm, filtered, concentrated to a filtrate weight of 14.2% and dried to a water content of 2.03wt% to obtain.

[0037] Resveratrol microcapsules: according to weight parts, 1 part of resveratrol and 2 parts of ethyl cellulose were added to 100 parts of dichloromethane, stirred at room temperature and 60rpm for 0.5 hours to obtain an oil phase; 2 parts of polyvinyl alcohol (PVA-1788) were added to 200 parts of deionized water, stirred at 75℃ and 80rpm for 0.4 hours to obtain an aqueous phase. The oil phase was added dropwise to the aqueous phase under stirring at 450rpm, and after the dropwise addition was completed, the emulsion was sheared at 12000rpm for 8 minutes to obtain an emulsion; then the emulsion was concentrated to 20% of the emulsion weight at 60℃ and 0.01MPa, and freeze-dried to a water content of 1.67wt% to obtain.

[0038] Example 1 The following test samples were prepared and then the whitening and freckle-removing effects of the test samples were detected.

[0039] Test sample 1: Magnolia biondii flower extract.

[0040] Test sample 2: Magnolia biondii flower extract and Jasminum grandiflorum extract were composed according to the weight ratio of 1:0.1.

[0041] Test sample 3: Magnolia biondii flower extract and Jasminum grandiflorum extract were composed according to the weight ratio of 1:0.3.

[0042] Test sample 4: Magnolia biondii flower extract and Jasminum grandiflorum extract were composed according to the weight ratio of 1:0.5.

[0043] Test sample 5: Magnolia biondii flower extract and Jasminum grandiflorum extract were composed according to the weight ratio of 1:1.

[0044] Test sample 6: Magnolia biondii flower extract and Jasminum grandiflorum extract were composed according to the weight ratio of 1:2.

[0045] Test sample 7: Magnolia biondii flower extract and Jasminum grandiflorum extract were composed according to the weight ratio of 1:3.

[0046] Test sample 8: Magnolia biondii flower extract and Jasminum grandiflorum extract were composed according to the weight ratio of 1:4.

[0047] Test sample 9: Magnolia biondii flower extract and Jasminum grandiflorum extract were composed according to the weight ratio of 1:8.

[0048] Test sample 10: Magnolia biondii flower extract and Jasminum grandiflorum extract were composed according to the weight ratio of 1:12.

[0049] Test sample 11: Jasminum grandiflorum extract.

[0050] The above samples were tested for inhibiting B16 cell melanin synthesis rate to verify the whitening and freckle-removing effect.

[0051] The testing method is as follows: C57BL / 6 mouse B16-F10 melanoma cells in logarithmic growth phase were used, and 1×10⁻⁶ cells were used as the test sample. 6 Cells were seeded at a concentration of 40 μg / mL in 6-well plates, with 2 mL of DEME medium added to each well. The plates were incubated at 37°C and 5% CO2 for 24 hours. The supernatant was discarded, and the cells were washed twice with PBS. For the test group, 2 mL of DEME medium containing 40 μg / mL of the test sample was added to each well. For the blank group, an equal volume of DEME medium was added. After incubation at 37°C and 5% CO2 for 48 hours, the supernatant was discarded, and the cells were digested and collected. Cells were centrifuged at 2000 rpm for 10 minutes. The supernatant was aspirated and 1 mL of sodium hydroxide solution containing 10 wt% DMSO (sodium hydroxide concentration 1 mol / L) was added. The cells were incubated at 80°C for 1 hour. The absorbance was measured at 405 nm using a microplate reader. The melanin inhibition rate δ was calculated as 100% × (OD200%). 0- OD1) / OD0; OD0 represents the absorbance of the blank group, and OD1 represents the absorbance of the test group; each group was tested in parallel four times, and the average value was taken. The results of the inhibition rate δ are shown in Table 1 and... Figure 1 Simultaneously, the increase in δ (K1 and K2) of each combination compared to the individual extracts of Magnolia officinalis and Jasminum nudiflorum were calculated.

[0052] Table 1: Melanin Inhibition Rate δ No. delta / % K1 K2 Test sample 1 51.26 / / Test sample 2 52.18 1.79% -2.27% Test sample 3 56.41 10.05% 5.66% Test sample 4 62.22 21.38% 16.54% Test sample 5 65.03 26.86% 21.80% Test sample 6 69.45 35.49% 30.08% Test sample 7 66.54 29.81% 24.63% Test sample 8 63.37 23.62% 18.69% Test sample 9 57.81 12.78% 8.28% Test sample 10 54.06 5.46% 1.25% Test sample 11 53.39 / / Tests show that the extracts of Magnolia biondii and Jasminum grandiflorum used in this invention are both effective in whitening and removing blemishes, inhibiting melanin synthesis by 51.26% and 53.39% respectively. When used in combination, they exhibit a positive correlation within a ratio of 1:0.1-12. Particularly within a ratio of 1:0.3-12, a synergistic effect is observed, while within a ratio of 1:0.5-4, the synergistic effect of whitening and removing blemishes is even stronger.

[0053] Example 2 Prepare the composition according to the contents of Table 2. The raw material quantities are in parts by weight.

[0054] Table 2: Composition of the composition Composition 1 2 3 4 5 Resveratrol microcapsule 0.08 0.07 0.08 0.08 0.08 Glabridin 0.08 0.09 0.08 0.08 0.08 Jasminum nudiflorum flower extract 0.9 1.1 0.9 0.9 0.9 Plumeria obtusa flower extract 1.3 1.2 1.3 1.3 1.3 Salix matsudana leaf extract 1.0 0.8 0 0 2.0 Equisetum arvense leaf extract 1.0 1.2 0 2.0 0 The preparation method of the above composition includes mixing raw materials, packaging and sterilizing to obtain the composition.

[0055] The performance of the above composition was tested. The test method is as follows: Logarithmic growth phase mouse macrophages RAW264.7 were collected and processed at a ratio of 5 × 10⁻⁶ cells / cells. 4 Inoculate with LPS at a concentration of 1 μg / mL into 6-well plates, add 2 mL of DEME medium to each well, and incubate at 37°C and 5% CO2 for 24 hours. After inoculation, remove the medium and divide the plate into groups: a model group, a positive drug group, and a composition group. The positive drug group received 2 mL of fresh DEME medium containing 1 μg / mL LPS and 100 μg / mL dexamethasone; the composition group received 2 mL of fresh DEME medium containing 1 μg / mL LPS and 150 μg / mL of the composition; and the model group received fresh DEME medium containing 1 μg / mL LPS. 2 mL of fresh DEME medium was incubated at 37℃ and 5% CO2 for 24 hours. The supernatant was collected, and the levels of inflammatory factors TNF-α and IL-6 in the supernatant were tested according to the ELISA kit instructions. Each group was tested in parallel for 4 times, and the average value was taken. The inhibition rate of the drug against the inflammatory factors TNF-α and IL-6 was calculated as follows: 100% × (model group content - test group content) / model group content, where the model group content represents the TNF-α and IL-6 content in the model group, and the test group content represents the TNF-α and IL-6 content in the positive drug group or the combination group. The test results are shown in Table 3 and... Figure 2 .

[0056] Table 3: Anti-inflammatory test No. TNF-α inhibition rate IL-6 inhibition rate Model group 76.38% 77.82% Composition 1 59.43% 61.01% Composition 2 58.02% 59.27% Composition 3 32.75% 34.54% Composition 4 52.14% 53.46% Composition 5 51.67% 52.93% As shown in Table 3, the composition prepared in this invention exhibits excellent anti-inflammatory effects. A comparison of compositions 1, 2, and 3 reveals that willowherb leaf extract and horsetail leaf extract effectively impart excellent anti-inflammatory effects, effectively soothing and repairing the skin. Tests on compositions 1, 4, and 5 show that using both willowherb leaf extract and horsetail leaf extract simultaneously in the composition results in better anti-inflammatory effects than using only one; the combination of the two in this invention promotes and synergistically enhances each other's effects.

[0057] The composition prepared by this invention has excellent properties and has broad application prospects in cosmetics.

[0058] Finally, it should be noted that the above-described embodiments are merely illustrative of specific implementations of the present invention, and while the descriptions are detailed, they should not be construed as limiting the scope of the present invention. It should be pointed out that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these modifications and improvements all fall within the scope of protection of the present invention.

Claims

1. A resveratrol composition having a whitening and freckle lightening effect, characterized by, The raw material composition comprises resveratrol microcapsules, glabridin, magnolia biondii flower extract, jasminum grandiflorum extract, epilobium angustifolium leaf extract and equisetum arvense leaf extract.

2. The resveratrol composition of claim 1, wherein, The weight ratio of the magnolia biondii flower extract and the jasminum grandiflorum extract is 1:0.5-4.

3. The resveratrol composition of claim 1, wherein, The preparation method of the resveratrol microcapsules comprises the following steps: dissolving resveratrol and ethyl cellulose in an organic solvent to obtain an oil phase; dissolving polyvinyl alcohol in water to obtain an aqueous phase; adding the oil phase into the aqueous phase drop by drop, and shearing and emulsifying to obtain an emulsion; concentrating and drying the emulsion to obtain the resveratrol microcapsules.

4. The resveratrol composition of claim 1, wherein, According to weight parts, the raw material composition comprises 0.05-0.5 parts of resveratrol microcapsules, 0.05-0.5 parts of glabridin, 0.1-1.5 parts of magnolia biondii flower extract, 0.1-1.5 parts of jasminum grandiflorum extract, 0.1-1.5 parts of epilobium angustifolium leaf extract and 0.1-1.5 parts of equisetum arvense leaf extract.

5. The resveratrol composition of claim 1, wherein, The magnolia biondii flower extract is obtained by heating extraction with an ethanol solution with a concentration of 30-50wt%.

6. The resveratrol composition of claim 1, wherein, The jasminum grandiflorum extract is obtained by heating extraction with an ethanol solution with a concentration of 35-55wt%.

7. The resveratrol composition of claim 1, wherein, The epilobium angustifolium leaf extract and the equisetum arvense leaf extract are both obtained by heating extraction with an ethanol solution with a concentration of 70-90wt%.

8. The resveratrol composition of claim 1, wherein, The weight ratio of the epilobium angustifolium leaf extract and the equisetum arvense leaf extract is 1:0.3-1.

2.

9. A method of preparing the resveratrol composition according to any one of claims 1-8, characterized in that, The method comprises mixing the raw materials, packaging and sterilizing.

10. Use of the resveratrol composition according to any one of claims 1-8 in the preparation of a whitening or anti-inflammatory cosmetic product, characterized in that, The amount of the resveratrol composition in the cosmetic is 0.01-50wt%.

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