Composition as well as preparation method and application thereof

Abstract

The invention discloses a composition as well as a preparation method and application thereof, and belongs to the technical field of beauty and skin care. The composition provided by the invention consists of the following components in percentage by mass: 1-20% of sodium polyglutamate; 1 to 20 percent of isosorbide dimethyl ether; 1-10% of a leontopodium alpinum extract; 1-5% of inositol; and the balance water. The composition provided by the invention can effectively improve mitochondrial functions and cell viability, and fundamentally solves skin problems. The invention also provides a preparation method and application of the composition.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetic skin care, in particular to a composition, a preparation method and application thereof. BACKGROUND

[0002] Mitochondria is the "energy factory" of cells, which is involved in ATP synthesis, oxidative stress regulation and apoptosis. Its dysfunction is closely related to skin problems.

[0003] Polyglutamic acid sodium (γ-PGA sodium) is a natural biopolymer, which has water solubility, non-toxicity, biodegradability and biocompatibility. Its molecule contains a large number of carboxyl and hydroxyl groups, which can bind water through hydrogen bonds, showing strong moisturizing and film forming properties. In addition, it also has antioxidant, anti-inflammatory and cell repair promoting functions. The related technology is widely used in the preparation of essence, mask and other products, which can improve skin quality by moisturizing, antioxidant and enhancing skin barrier function. Traditional research shows that polyglutamic acid sodium alone does not have the direct regulation function of mitochondria function.

[0004] Therefore, it is very important to design a composition that can improve the function of mitochondria. SUMMARY

[0005] The present application aims to at least solve one of the technical problems existing in the prior art. To this end, the present application provides a composition which can effectively improve the function of mitochondria and cell viability.

[0006] The present application also provides a preparation method of the above-mentioned composition.

[0007] The present application also provides a skin care product comprising the above-mentioned composition.

[0008] The present application also provides a preparation method of the above-mentioned skin care product.

[0009] According to the embodiment of the first aspect of the present application, a composition is provided, which consists of the following components in mass percentage:

[0010]

[0011] The composition according to the embodiment of the present application has at least the following beneficial effects:

[0012] The composition provided by the present application has the functions of moisturizing, antioxidation and enhancing skin barrier; the isosorbide dimethyl ether has the effect of promoting skin penetration of effective components; the Leontopodium alpinum extract has the effect of inhibiting the generation of melanin and tyrosinase, and the inositol has the effects of promoting the generation of hyaluronic acid and collagen and promoting the penetration of other effective components; after the combination of the above components, the problem of poor transdermal absorption of polyglutamic acid sodium is effectively solved, so that it is more easily absorbed, thereby maximizing the effects of moisturizing, soothing, repairing, anti-wrinkle and firming; and after the active ingredient combination penetrates into the skin, a new effect is produced: improving mitochondrial function, specifically improving mitochondrial antioxidant capacity and ATP energy, activating mitochondrial network, reducing the generation of free radicals from the inside to the outside, and improving mitochondrial function from the source to repair skin barrier and solve various skin problems.

[0013] According to some embodiments of the present application, the Leontopodium alpinum extract includes at least one of a flower extract and a leaf extract of Leontopodium alpinum.

[0014] According to some embodiments of the present application, in the composition, the mass percentage of the polyglutamic acid sodium is 1-20%; for example, it can be about 3%, 5%, 8%, 10% or about 15% specifically. In actual production, as long as the polyglutamic acid sodium from the market is suitable; in order to better improve the skin care effect, polyglutamic acid sodium with a molecular weight of 700-800 million can be selected; and in this molecular weight range, the performance of the composition is little affected.

[0015] According to some embodiments of the present application, in the composition, the mass percentage of the isosorbide dimethyl ether is 1-20%; for example, it can be about 3%, 5%, 8%, 10% or about 15% specifically.

[0016] According to some embodiments of the present application, in the composition, the mass percentage of the Leontopodium alpinum extract is 1-10%; for example, it can be about 2%, 3%, 5% or about 8% specifically.

[0017] According to some embodiments of the present application, in the composition, the mass percentage of the inositol is 1-5%; for example, it can be about 2%, 3% or about 4% specifically.

[0018] According to some embodiments of the present application, the composition consists of the following components by mass percentage:

[0019]

[0020] According to some embodiments of the present application, the composition consists of the following components by mass percentage:

[0021]

[0022] According to the embodiments of the second aspect of the present application, a preparation method of the composition of the embodiments of the first aspect of the present application is provided, and the preparation method comprises the following steps:

[0023] S1. mixing the sodium polyglutamate, isosorbide dimethyl ether and part of water, and then heat treating to obtain a mixture A;

[0024] mixing the high-mountain edelweiss extract, inositol and the rest of water to obtain a mixture B;

[0025] S2. mixing the mixture A and the mixture B.

[0026] Since the preparation method adopts all the technical solutions of the composition of the above embodiments, it at least has all the beneficial effects brought by the technical solutions of the above embodiments. Further,

[0027] Since the high-mountain edelweiss extract and inositol will be inactivated to a certain extent after heating, and the sodium polyglutamate and other components need to be fully dissolved and dispersed under heating conditions, the preparation method provided by the present application separately processes the two parts of the preparation raw materials, significantly improves the dispersion uniformity between the preparation raw materials on the basis of ensuring activity.

[0028] According to some embodiments of the present application, in step S1, the part of water accounts for 45-60% of the mass percentage of the composition. Specifically, it is the balance of water in the mixture B.

[0029] According to some embodiments of the present application, in step S1, the rest of water accounts for 18-22% of the mass percentage of the composition. For example, it can be about 20%.

[0030] According to some embodiments of the present application, in step S1, the mixing of the mixture A is prepared, and the end point is the dissolution of the sodium polyglutamate and isosorbide dimethyl ether. In actual production, if necessary, mass transfer methods such as stirring and ultrasonic can be added, and the temperature can also be appropriately increased; specifically, it can be increased to 75-85°C; further specifically, it can be about 80°C.

[0031] According to some embodiments of the present application, in step S1, the temperature of the heat treatment is 115-125°C. For example, it can be about 120°C or about 121°C. Thus, preliminary sterilization is achieved.

[0032] According to some embodiments of the present application, in step S1, the heat treatment time is 15-30 min. For example, it can be about 18 min, 20 min, 22 min or about 25 min.

[0033] According to some embodiments of the present application, step S2 is operated under sterile conditions. In this way, the composition can be prevented from being contaminated by bacteria and mold, and the performance test results of the composition can be prevented from being affected by the above-mentioned miscellaneous bacteria.

[0034] According to some embodiments of the present application, in step S2, the mixing comprises adding the mixture B to the mixture A by sterile filtration and stirring. In actual production, only the mixture B after sterile filtration and the mixture A need to be mixed, and the feeding sequence of the two does not obviously affect the performance of the obtained composition; the present application selects to add the mixture B to the mixture A during filtration in terms of operational simplicity.

[0035] According to some embodiments of the third aspect of the present application, a skin care product is provided, which comprises the composition according to the first aspect of the present application.

[0036] Since the skin care product adopts all the technical solutions of the composition according to the above-mentioned embodiments, at least all the beneficial effects brought by the technical solutions of the above-mentioned embodiments are achieved.

[0037] According to some embodiments of the present application, in the skin care product, the mass percentage of the composition is 0.5-20%. For example, it can be about 0.8%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, 5.5%, 6%, 8%, 10%, 12%, 14%, 16% or about 18%.

[0038] According to some embodiments of the present application, the skin care product further comprises a polyol, a chelating agent, a thickening agent, a preservative, a pH adjusting agent, 1,2-hexanediol and water. In actual production, the types and amounts of the above-mentioned auxiliary materials can be adjusted according to the dosage form and skin feel of the required skin care product. The present application does not make strict limitations.

[0039] According to some embodiments of the present application, the polyol comprises at least one of glycerol, butylene glycol, propylene glycol and diglycerol. For example, it can be a mixture of glycerol and butylene glycol. Further specifically, the mass ratio of the glycerol and butylene glycol is 1-2:1; for example, it can be about 1.5:1 or about 1.6:1.

[0040] According to some embodiments of the present application, in the skin care product, the mass percentage of the polyol is 1-20%; for example, it can be 10-15%; further specifically, it can be about 12% or about 13%.

[0041] According to some embodiments of the present application, the chelating agent comprises at least one of disodium EDTA, trisodium EDTA and tetrasodium EDTA.

[0042] According to some embodiments of the present application, the chelating agent has a mass percentage of 0.05-2% in the skin care product; for example, it can be about 0.1%, 0.5% or about 1%.

[0043] According to some embodiments of the present application, the thickening agent comprises at least one of hydroxyethyl acrylate-sodium acryloyldimethyl taurate copolymer, polyacrylate crosspolymer-6, carbomer, magnesium silicate, magnesium aluminum silicate, xanthan gum and hydroxyethyl cellulose.

[0044] According to some embodiments of the present application, the thickening agent is a mixture of xanthan gum and carbomer. The ratio of the xanthan gum to the carbomer is 1:1-2; for example, it can be about 1:1.5.

[0045] According to some embodiments of the present application, the thickening agent has a mass percentage of 0.1-1% in the skin care product; for example, it can be about 0.2%, 0.3%, 0.5% or about 0.8%.

[0046] According to some embodiments of the present application, the preservative comprises at least one of methylparaben, p-hydroxyacetophenone and chlorphenesin.

[0047] According to some embodiments of the present application, the preservative has a mass percentage of 0.2-1% in the skin care product; for example, it can be about 0.3%, 0.5% or about 0.8%.

[0048] According to some embodiments of the present application, the pH regulator comprises at least one of triethanolamine, arginine, sodium hydroxide and ammonium hydroxide.

[0049] According to some embodiments of the present application, the pH regulator has a mass percentage of 0.1-0.2% in the skin care product; for example, it can be about 0.15%. Further, the pH regulator is used to adjust the pH of the skin care product to 5.0-7.0; in actual production, if the pH and the mass percentage conflict, the specific pH is used as the criterion.

[0050] According to some embodiments of the present application, the 1,2-hexanediol has a mass percentage of 1-3% in the skin care product; for example, it can be about 1.2%, 1.5%, 2% or about 2.5%. The 1,2-hexanediol has a synergistic antibacterial effect, which is different from the solvent and lubricating effect of the polyhydric alcohol.

[0051] According to some embodiments of the present application, the skin care product comprises the following components by mass percentage:

[0052]

[0053] According to the embodiment of the fourth aspect of the present application, a method for preparing the skin care product of the embodiment of the third aspect of the present application is provided, and the method comprises mixing the components of the skin care product.

[0054] Since the method for preparing the skin care product of the above embodiment adopts all the technical solutions of the skin care product of the above embodiment, it has all the beneficial effects brought by the technical solutions of the above embodiment.

[0055] According to some embodiments of the present application, the method for preparing the skin care product comprises the following steps:

[0056] D1. heating and mixing the remaining amount of water, polyhydric alcohol, chelating agent and thickening agent;

[0057] D2. cooling the mixture obtained in step D1 and mixing the preservative;

[0058] D3. cooling the mixture obtained in step D2 and mixing the pH additive;

[0059] D4. cooling the mixture obtained in step D3 and mixing the composition and 1,2-hexanediol.

[0060] According to some embodiments of the present application, in step D1, the temperature of the heating and mixing is 85-90°C. Within this temperature range, on the one hand, it is beneficial to perform preliminary sterilization, and on the other hand, it is helpful for the mixing and dispersion of the thickening agent and the like.

[0061] According to some embodiments of the present application, in step D1, the heating and mixing comprises sequentially stirring and heating to the target temperature, holding and stirring, and homogenizing. The holding and stirring is performed for 15-25 min, for example, about 20 min. The homogenizing is performed for 2-3 min. In actual production, the frequency of stirring and homogenizing is not strictly limited, as long as uniform mixing can be achieved.

[0062] According to some embodiments of the present application, in step D2, the temperature after cooling is 65-75°C. For example, it can be about 70°C.

[0063] According to some embodiments of the present application, in step D3, the temperature after cooling is 55-63°C. For example, it can be about 60°C.

[0064] According to some embodiments of the present application, in step D4, the temperature after cooling is 35-45°C. For example, it can be about 40°C.

[0065] According to some embodiments of the present application, in step D4, the mixing comprises sequentially adding the composition and the 1,2-hexanediol to the mixture obtained in step D3.

[0066] If no special description, the mixing or other mass transfer steps in the present application are carried out under stirring, the stirring conditions are not strictly limited.

[0067] If no special description, the "about" in the present application actually means that the allowable error is within ±2%, for example, about 100 actually means 100±2%×100.

[0068] Other features and advantages of the present application will be set forth in the following description, and in part will become apparent from the description, or can be learned by practice of the present application. DETAILED DESCRIPTION

[0069] The concept and the technical effects of the present application will be described below in combination with the embodiments, so as to fully understand the purpose, features and effects of the present application. Obviously, the described embodiments are only some of the embodiments of the present application, but not all the embodiments. Based on the embodiments of the present application, other embodiments obtained by those skilled in the art without creative labor are within the protection scope of the present application.

[0070] In the description of the present application, the description of the terms "one embodiment", "some embodiments", "exemplary embodiment", "example", "specific example", or "some examples" means that the specific features, structures, materials or characteristics described in combination with the embodiment or example are contained in at least one embodiment or example of the present application. In the present specification, the exemplary description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the described specific features, structures, materials or characteristics can be combined in any one or more embodiments or examples in a suitable manner.

[0071] Embodiment 1

[0072] According to the proportioning of Table 1, a composition is prepared in this example, and the specific steps are as follows:

[0073] S1. Mix sodium polyglutamate, isosorbide dimethyl ether with the remaining amount of water, and perform high temperature treatment under the condition of 121℃, 20min, then wait for it to cool down to room temperature to obtain mixture A;

[0074] Mix the high mountain edelweiss flower / leaf extract, inositol and 20% of water in the total amount of the composition, and stir until dissolved uniformly to obtain mixture B;

[0075] S2. Under sterile conditions, add mixture B to mixture A by sterile filtration, and stir to disperse uniformly, to obtain the composition.

[0076] Embodiments 2-3 and Comparative Examples 1-2 each prepare a composition, and the difference from Embodiment 1 is as follows:

[0077] The raw materials are slightly different, and the specific differences are shown in Table 1.

[0078] Table 1: Proportions of compositions in Examples 1-3 and Comparative Examples 1-2 (mass percent)

[0079]

[0080] Example 4

[0081] Referring to the formulation provided in Table 2, a skin care product, specifically an emulsion, is prepared according to the following method:

[0082] D1. Add water, polyol, chelating agent, and thickening agent in sequence into an emulsifying kettle, and heat and stir until the temperature reaches 85-90°C (within this range, the temperature can vary, and the performance of the obtained skin care product will not be affected, and the other range values in this example are explained similarly), and maintain the temperature and stir for 20 min, and then homogenize for 2-3 min;

[0083] D2. Cool the product obtained in step D1 to about 70°C, add p-hydroxyacetophenone, and stir until it is dissolved uniformly;

[0084] D3. Continue to cool the mixture obtained in step D2 to about 60°C, add the pH adjuster, and stir until it is dissolved uniformly;

[0085] D4. Continue to cool the mixture obtained in step D3 to about 40°C, add the composition and 1,2-hexanediol in sequence, and stir until they are uniformly mixed.

[0086] Table 2: Components of skin care products in Examples 4-8 (mass percent)

[0087]

[0088]

[0089] Comparative Example 3

[0090] In this example, a skin care product is prepared, which is different from Example 6 in that:

[0091] In step D4, the composition is not added; instead, an equal amount of sodium polyglutamate, isosorbide dimethyl ether, high mountain edelweiss flower / leaf extract, inositol, and water are directly added. That is, the composition is not prepared in advance.

[0092] Comparative Example 4

[0093] In this example, a skin care product is prepared, which is different from Example 6 in that:

[0094] In step D4, the composition is not added; instead, an equal amount of sodium polyglutamate, isosorbide dimethyl ether, high mountain edelweiss flower / leaf extract, inositol, and water are directly added. That is, the composition is not prepared in advance.

[0095] Test Example 1

[0096] This example tests the performance of the compositions obtained in Examples 1-3 and Comparative Examples 1-2, specifically cell viability and mitochondrial ATP production level. The raw material preparation is as follows: HFF-1 cells are cultured in DMEM medium (containing 1% penicillin-streptomycin, 10% FBS, purchased from Thermo, USA) at 37°C, 5% CO2, when the cells grow to about 80%, trypsin with a concentration of 0.25% is used for digestion, and finally a certain concentration of cell suspension is prepared for subculture.

[0097] The test method for cell viability is as follows: logarithmic growth phase HFF-1 cells are inoculated in a 96-well plate with a density of 5x10 4 The cells are divided into a normal control group (NC), a model control group (MC), and an experimental group. The cells in the normal control group are not treated; the cells in the model control group are added with H2O2 with a final concentration of 900 μmol / L; the cells in the experimental group are added with H2O2 with a final concentration of 900 μmol / L, and then added with the compositions obtained in Examples 1-3 and Comparative Examples 1-2 with a final concentration of 20 mg / mL (diluted with water to simulate the concentration in specific skin care products); the three groups of samples are incubated in an incubator for 4 h. Finally, Prestoblue detection reagent is added, and the fluorescence intensity RFU is detected at 570 nm using a multifunctional enzyme label instrument, with 4 repeats for each group. The cell metabolic activity calculation formula is as follows:

[0098]

[0099] Among them, the blank hole refers to the hole without cells, which is to exclude the detection interference. The calculation results of cell viability are shown in Table 3.

[0100] The test method for verifying mitochondrial ATP production level is as follows: logarithmic growth phase HFF-1 cells are inoculated in a cell 6-well plate with a density of 1.5x10 5The cells were incubated at 37℃ in a 5% CO2 incubator for 12 hours. Then, the cells were divided into a normal control (NC) group, a model control (MC) group, and an experimental group. The cells in the normal control group were not treated. The cells in the model control group were added with H2O2 at a final concentration of 900 μmol / L. The cells in the experimental group were added with H2O2 at a final concentration of 900 μmol / L, and then added with the compositions obtained in Examples 1 to 3 and Comparative Examples 1 and 2 at a final concentration of 20 mg / mL (diluted with water to simulate the concentration in a specific skin care product). The cells were incubated in the incubator for 4 hours. Then, the supernatant was removed, and a Ripa lysis solution was added for lysis. The cell lysate was collected in a 1.5 mL centrifuge tube, centrifuged at 12,000 g (relative centrifugal force) at 4℃ for 5 minutes, and the supernatant was taken for standby. 100 μL of ATP detection working solution was added to a black 96-well plate, and the plate was placed at room temperature for 3 to 5 minutes. 20 μL of sample (supernatant) or standard was added to the detection well, and the plate was mixed quickly. The RLUs were measured by a multifunctional enzyme label instrument, and the ATP content of each group was calculated according to the concentration-RLU value curve, with 4 repeats for each group.

[0101] In addition, according to the BCA protein concentration determination kit instructions, the absorbance of each group of samples at 562 nm was detected on a multifunctional enzyme label instrument, and the protein concentration of the sample to be tested was calculated according to the concentration-absorbance curve, with 4 repeats for each group.

[0102]

[0103] The test results of the ATP concentration are shown in Table 3.

[0104] Table 3 Performance of the compositions obtained in Examples 1 to 3 and Comparative Examples 1 and 2

[0105] Cell viability levels ATP content Model control group 59.98％±1.08％ 51.78％±2.18％ Example 1 70.61％±1.56％ 66.21％±1.44％ Example 2 102.49±2.43％ 101.41±1.59％ Example 3 68.02％±1.78％ 65.27％±2.11％ Comparative Example 1 62.57％±2.01％ 60.32％±1.86％ Comparative Example 2 60.52％±0.88％ 57.63％±1.27％

[0106] The results in Table 3 are the results of further calculation of the results of the above two formulas. The specific calculation method is that the data of the model control group is the percentage of the data of the normal control group; and the examples and comparative examples are the improvement ratios compared with the model control group.

[0107] As can be seen from the results of Comparative Examples 1 to 3, within the scope provided by the present application, changing the mass ratio of each component in the composition can significantly help improve the cell viability level and the content of mitochondrial ATP. However, if the content of isosorbide dimethyl ether is significantly reduced, the permeability of the cells will decrease to some extent, which will affect the action of other components.

[0108] The results of Comparative Example 2 and Comparative Examples 1-2 show that polyglutamic acid sodium alone or other ingredients alone have certain effects on the improvement of cell viability level or ATP content, but compared with Example 2, the effects are significantly reduced, thus indicating that the composition provided in the application has significant synergistic effects between the ingredients, and can more activate the protection of cells and the promotion of mitochondrial ATP synthesis in oxidative damage, and has an important role in improving cell viability and mitochondrial energy metabolism.

[0109] Test Example 2

[0110] This example verifies the efficacy of the skin care products obtained in Examples 4-8 and Comparative Examples 3-4, and the test method is as follows: 200 μL of the skin care product stock solution, 200 μL of hyaluronidase solution (100 U) and 200 μL of sodium hyaluronate (0.04 wt%) are mixed, and incubated at 37°C for 20 min; 200 μL of CTAB solution (1 mg / mL) is added; 200 μL of each reaction solution is taken and added to a 96-well enzyme-labeled plate, and the absorbance is measured at 600 nm. The above group is called T; this example also tests the absorbance of groups T0, C and C0, wherein the difference between T0 and T is that the hyaluronidase solution is replaced with an equal amount of PBS buffer; the difference between C and T is that the skin care product stock solution is replaced with an equal amount of water; the difference between C0 and C is that the hyaluronidase solution is replaced with an equal amount of PBS buffer. The effect of the skin care product obtained in the example or comparative example on the activity of hyaluronidase is calculated. The calculation method of the hyaluronidase inhibition rate is as follows:

[0111] Hyaluronidase inhibition rate (%) = 1 - (T0-T) / (C0-C), wherein T0, T, C0 and C represent the absorbance at 600 nm.

[0112] The test results are shown in Table 4.

[0113] Table 4 Efficacy of skin care products obtained in examples and comparative examples

[0114] Group Hyaluronidase inhibition rate Example 4 58.14％ Example 5 63.68％ Example 6 84.56％ Example 7 85.87％ Example 8 88.02％ Comparative Example 3 60.25％ Comparative Example 4 42.51％

[0115] The results of Comparative Examples 4-8 show that as the content of the composition in the skin care product increases, the inhibition rate of hyaluronidase in the skin care product is significantly improved; that is, the improvement effect on mitochondrial function gradually increases. However, when the addition ratio of the composition is >2%, the inhibition rate of hyaluronidase increases, but the increase rate is very slow, and therefore, about 2% can be added to solve the problem, which is relatively economical and applicable; in actual production, about 2% can also be added.

[0116] The results of Comparative Example 6 and Comparative Examples 3-4 show that sodium polyglutamate alone has limited inhibitory effect on hyaluronidase, and if the components of the composition are not prepared in advance but are added directly in the preparation process of the skin care product, the inhibitory effect of the resulting skin care product on hyaluronidase will be significantly reduced. The reason is that during the preparation process of the composition, especially during the heating process, there will be some interaction between sodium polyglutamate and dimethyl isosorbide, which will promote the skin penetration process of the former; further, compared with the skin care product, the concentration of the preparation raw materials of the composition system, such as high-mountain edelweiss flower / leaf extract, is higher, which is also more conducive to the interaction between them and synergistically improve the skin care effect.

[0117] Therefore, it is shown that the skin care product comprising the composition provided by the present application can significantly improve the skin care effect by improving the function of mitochondria, and there is a significant synergistic effect between the preparation process of the composition and the preparation raw materials.

[0118] Test Example 3

[0119] In this example, the skin care product obtained in Example 6 was used as a sample to conduct a human efficacy evaluation test to further verify its effect. The process and results of the efficacy verification are as follows:

[0120] According to the "Cosmetic Efficacy Claim Evaluation Standard", at least 30 qualified subjects were recruited to use the test sample continuously for 28 days for human efficacy evaluation test. During the test process, 30 subjects were enrolled, and 0 dropped out (dropped out means terminated the test in advance), and finally 30 subjects met the scheme and were effective cases. Among them, 24 were women and 6 were men, with an average age of 42.27 years. After cleansing, the subjects took an appropriate amount of the skin care product obtained in Example 6 and applied it to the test site and massaged until absorption, once a day in the morning and evening, for 28 consecutive days. The test used a before-and-after control method, and the skin of the test area of the subjects before and after using the test skin care product was tested by instrument testing, image acquisition, and dermatologist evaluation. The test items included transepidermal water loss, skin stratum corneum moisture content, facial red zone value, and EI value, and data statistical analysis was conducted to evaluate the repair, soothing, and moisturizing efficacy of the skin care product obtained in Example 6 after use.

[0121] The test results show that after 28 days of continuous use: ① the skin trans-epidermal water loss rate of the subjects has statistical significance compared with the skin trans-epidermal water loss rate of the base value (0.01 < P < 0.05), and the improvement rate is 19.68%; ② the skin stratum corneum water content of the subjects has a very significant difference compared with the skin stratum corneum water content of the base value (P < 0.001), and the improvement rate is 43.69%; ③ the facial red zone value of the subjects has statistical significance compared with the facial red zone value of the base value (0.01 < P < 0.05), and the improvement rate is 12.72%; the EI value of the subjects has a very significant difference compared with the EI value of the base value (P < 0.001), and the improvement rate is 18.42%; ④ the self-evaluation of the subjects (D28): 100.00% of the subjects are satisfied with the use of the product; 100.00% of the subjects think that the skin desquamation is improved; 96.67% of the subjects think that the skin redness is improved; 96.67% of the subjects think that the skin stinging is improved; 96.67% of the subjects think that the skin burning is improved; 96.67% of the subjects think that the skin itching is improved; 100.00% of the subjects think that the skin dryness is improved; 100.00% of the subjects think that the skin tolerance is improved; 100.00% of the subjects think that the skin roughness is improved; 100.00% of the subjects think that the skin barrier is improved; and 100.00% of the subjects think that the skin overall is improved. According to the above results, it can be known that the skin care product obtained in Example 6 has obvious effects in repair, moisturizing, soothing and the like.

[0122] In summary, the composition prepared by the present application has obvious effects on improving the efficacy of mitochondria and cell viability, the skin care product comprising the above composition has obvious inhibitory efficiency on hyaluronidase, and the satisfaction degree of the subjects is high; in combination with comparative analysis of all results, it can be known that there is a significant synergistic effect between different components in the composition provided by the present application, which can act on the nature of mitochondria, and then has a good conditioning effect on the skin.

[0123] The above has made a detailed description of the embodiments of the present application, but the present application is not limited to the above embodiments, and various changes can be made within the knowledge range of ordinary skilled persons in the art without departing from the purpose of the present application. In addition, the embodiments of the present application and the features in the embodiments can be combined with each other without conflict.

Claims

1. A composition characterized in that, The composition consists of the following components by mass percentage:

2. The composition of claim 1, wherein, The high mountain fire extraction includes at least one of flower extract and leaf extract of high mountain fire.

3. The composition according to claim 1 or 2, characterized in that, The composition consists of the following components by mass percentage:

4. A process for the preparation of a composition according to any one of claims 1 to 3, characterised in that, The preparation method includes the following steps: S1. Heat treating the mixture of sodium polyglutamate, isosorbide dimethyl ether and part of water to obtain mixture A; Mixing the high mountain fire extraction, inositol and the rest of water to obtain mixture B; S2. Mixing mixture A and mixture B.

5. The production method according to claim 4, characterized by, In step S1, the part of water accounts for 45-60% of the mass percentage of the composition.

6. The preparation method according to claim 4, characterized in that, In step S1, the temperature of the heat treatment is 115-125℃; and / or, in step S1, the duration of the heat treatment is 15-30 min.

7. A skin care product comprising, The skin care product includes the composition of any one of claims 1-3.

8. The skin care product of claim 7, wherein, In the skin care product, the mass percentage of the composition is 0.5-20%.

9. The skin care product according to claim 7 or claim 8, wherein the skin care product is a cosmetic product. The skin care product further includes polyol, chelating agent, thickening agent, p-hydroxyacetophenone, pH adjusting agent, 1,2-hexanediol and water; And / or, the skin care product consists of the following components by mass percentage:

10. A method of formulating a skin care product according to any one of claims 7 to 9, characterised in that, The preparation method includes mixing the components of the skin care product.

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