Camellia nitidissima fermentation liquor for inhibiting cortisol secretion as well as preparation method and application thereof
By encapsulating flavonoids with lactic acid bacteria and boron cluster-based materials from the fermentation liquid of Camellia chrysanthemi, cosmetics that inhibit cortisol secretion were prepared, solving the skin sensitivity reaction caused by excessive cortisol secretion and achieving effective cortisol inhibition and skin soothing effects.
Patent Information
- Application Number
- CN202511137328.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-08-14
- Publication Date
- 2025-11-07
AI Technical Summary
Excessive secretion of cortisol leads to skin sensitivity reactions such as dryness and erythema, and current technologies are unable to effectively inhibit its secretion.
Cosmetics that inhibit cortisol secretion were prepared by using Camellia chrysanthemi fermentation broth, through lactic acid bacteria fermentation and encapsulation of flavonoids with boron cluster-based materials. The flavonoids competitively bind to 11β-hydroxysteroid dehydrogenase type 1, reducing the conversion of cortisol precursors to active cortisol. Furthermore, the inhibitory effect is synergistically enhanced by neutralizing reactive oxygen species and blocking the release of inflammatory factors through 2-hydroxysaccharin.
It effectively reduces excessive cortisol secretion, improves skin problems caused by excessive cortisol secretion, provides a soothing environment, and is non-cytotoxic.
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Abstract
Description
TECHNICAL FIELD
[0001] The application relates to the cosmetic technology field, in particular to a camellia sinensis var. assamica fermentation liquor for inhibiting secretion of cortisol as well as a preparation method and application thereof. BACKGROUND
[0002] With the improvement of living standards, the inner concerns of the general consumers gradually focus on self-cognition. The consumers gradually realize that there is a certain correlation between their emotions and skin. When feeling happy and relaxed for a long time, the skin usually presents a healthy and bright luster. When being in anxiety, stress or depression for a long time, the skin may have various problems.
[0003] When the human body perceives negative emotions such as tension, anxiety and isolation, the hypothalamic-pituitary-adrenal axis in the human body is activated, further affecting other physiological systems in the body to resist stress. In the regulation process, cortisol, a related response glucocorticoid, is regarded as a "stress hormone" and "emotional factor". Excessive expression of cortisol can cause skin sensitive reactions such as dryness and erythema. SUMMARY
[0004] In order to improve the problem of excessive conversion of cortisol in the skin, the application provides a camellia sinensis var. assamica fermentation liquor for inhibiting secretion of cortisol as well as a preparation method and application thereof.
[0005] In a first aspect, the application provides a preparation method of a camellia sinensis var. assamica fermentation liquor for inhibiting secretion of cortisol, which adopts the following technical scheme:
[0006] A preparation method of a camellia sinensis var. assamica fermentation liquor for inhibiting secretion of cortisol, comprising the following steps:
[0007] S1: inoculating lactic acid bacteria into a culture medium, constant-temperature oscillation culture to obtain a seed liquid;
[0008] S2: crushing and sieving camellia sinensis var. assamica to obtain camellia sinensis var. assamica powder;
[0009] S3: adding 0.8-1.2% camellia sinensis var. assamica powder, 0.01-0.1% inhibitor and the rest deionized water into a fermentation tank for sterilization;
[0010] S4: inoculating the seed liquid into the fermentation tank for constant-temperature culture to obtain a fermentation liquor;
[0011] S5: filtering and sterilizing the fermentation liquor, and adding a preservative to obtain the camellia sinensis var. assamica fermentation liquor;
[0012] The inhibitor is a boron cluster-based material wrapped flavonoid compound.
[0013] As a natural plant, Camellia sinensis contains many bioactive substances such as polyphenols, flavonoids, and amino acids. These components have the potential to regulate physiological metabolism in plants. The secretion of cortisol is closely related to the body's inflammatory response and hormone regulation pathways.
[0014] Flavonoids can bind to 11β-hydroxysteroid dehydrogenase type 1 competitively, making it difficult for cortisol precursors to convert into active cortisol, affecting the regulation process of the hypothalamic-pituitary-adrenal axis, thereby reducing the excessive secretion of cortisol. Boron cluster-based materials can help protect flavonoids from being destroyed by the fermentation environment. The endogenous components of Camellia sinensis and exogenous flavonoids work synergistically to further enhance the inhibitory effect of cortisol.
[0015] Preferably, in step S4, the seed solution is inoculated into the fermenter at an inoculation amount of 6-7.32%, and cultured at 23-27℃ with 150-250r / min oscillation for 20-22h.
[0016] A low inoculation amount may cause slow bacterial growth and prolong the fermentation period, while a high inoculation amount may trigger nutrient competition and lead to the accumulation of metabolic byproducts. Setting the fermentation temperature lower than the conventional fermentation temperature helps reduce the degradation of heat-sensitive substances such as polyphenols and flavonoids in Camellia sinensis. Lactic acid bacteria can maintain their activity in this temperature range without over-reproduction, which can cause system imbalance. Moderate oscillation provides dissolved oxygen and promotes uniform mixing of materials, allowing lactic acid bacteria to fully contact Camellia sinensis components and accelerate the reaction process. This culture duration is beneficial for the fermentation to proceed fully, allowing lactic acid bacteria to complete the conversion of Camellia sinensis components.
[0017] Preferably, the inhibitor is 2-hydroxymercury salicylate coated with a boron cluster-based material.
[0018] The phenolic hydroxyl group of 2-hydroxymercury salicylate can neutralize active oxygen and reduce oxidative stress-induced activation of the hypothalamic-pituitary-adrenal axis. Additionally, 2-hydroxymercury salicylate can indirectly reduce the overreaction of the hypothalamic-pituitary-adrenal axis by blocking the release of inflammatory factors. The active ingredients such as polyphenols and amino acids in Camellia sinensis can provide a soothing environment for the skin. As an inhibitor, 2-hydroxymercury salicylate can specifically enhance the inhibitory effect on cortisol secretion. The synergistic effect of the two can further improve skin problems caused by excessive cortisol secretion.
[0019] Preferably, the inhibitor is 2-hydroxymercury salicylate coated with a boron cluster-based material.
[0020] Since the sodium dodecahydrododecaborate has a stable closed cage boron hydride anion structure, its rigid cage skeleton can effectively wrap the 2-hydroxychlorophenol to form a protective barrier, so that the 2-hydroxychlorophenol is not easily destroyed by lactic acid bacteria metabolic enzymes or structurally degraded due to environmental factors during fermentation, thereby retaining the activity of inhibiting cortisol secretion.
[0021] Preferably, the inhibitor comprises the following raw materials: sodium dodecahydrododecaborate, 2-hydroxychlorophenol, and an ethanol aqueous solution.
[0022] Preferably, the preparation method of the inhibitor is: dissolving sodium dodecahydrododecaborate and 2-hydroxychlorophenol in an ethanol aqueous solution, stirring and reacting at 23-27°C for 10-14h, and then filtering and freeze-drying.
[0023] Long-time gentle stirring is conducive to the gradual embedding of 2-hydroxychlorophenol into the cavity of sodium dodecahydrododecaborate, and is not prone to molecular structure destruction caused by violent mixing. The use of freeze-dried form is conducive to avoiding the precipitation of 2-hydroxychlorophenol or the collapse of boron cluster structure caused by high-temperature drying.
[0024] Preferably, the mass ratio of sodium dodecahydrododecaborate to 2-hydroxychlorophenol is (1-2):1.
[0025] When the mass ratio of sodium dodecahydrododecaborate to 2-hydroxychlorophenol is (1-2):1, it is conducive to realizing that the rigid cage structure of sodium dodecahydrododecaborate fully wraps 2-hydroxychlorophenol to form a stable protective barrier, avoiding its destruction or degradation during fermentation. This mass ratio can not only ensure that the active site of 2-hydroxychlorophenol is exposed to play a role in inhibiting cortisol secretion, but also is not prone to incomplete wrapping caused by unbalanced ratio.
[0026] In a second aspect, the present application provides a camellia sinensis fermentation liquor for inhibiting cortisol secretion, which adopts the following technical solution:
[0027] A camellia sinensis fermentation liquor for inhibiting cortisol secretion is prepared by the above preparation method.
[0028] In a third aspect, the present application provides a cosmetic for inhibiting cortisol secretion, which adopts the following technical solution:
[0029] A cosmetic for inhibiting cortisol secretion contains the above camellia sinensis fermentation liquor as the main active ingredient or the only active ingredient.
[0030] In summary, the present application has the following beneficial effects:
[0031] 1. As a natural plant, Camellia chrysantha contains a variety of bioactive substances such as polyphenols, flavonoids, and amino acids. These components have the potential to regulate physiological metabolism in plants, and the secretion of cortisol is closely related to the body's inflammatory response and hormone regulation pathways.
[0032] Flavonoids can competitively bind to 11β-hydroxysteroid dehydrogenase type 1, making it difficult for cortisol precursors to be converted into active cortisol, thus affecting the regulation of the hypothalamic-pituitary-adrenal axis and reducing excessive cortisol secretion. Boron cluster-based materials help protect flavonoids from being destroyed by the fermentation environment. The endogenous components of Camellia chrysantha and exogenous flavonoids work synergistically to further enhance the cortisol inhibitory effect.
[0033] 2. Because too low an inoculation amount may lead to slow bacterial growth and prolong the fermentation cycle, while too high an inoculation amount may cause nutrient competition and lead to the accumulation of metabolic byproducts, setting the fermentation temperature lower than the conventional fermentation temperature is beneficial to reduce the degradation of heat-sensitive substances such as polyphenols and flavonoids in Camellia chrysantha. Within this temperature range, lactic acid bacteria can maintain their activity without over-proliferating and causing system imbalance. Moderate shaking is beneficial to provide dissolved oxygen and promotes uniform mixing of materials, allowing the lactic acid bacteria to fully contact the components of Camellia chrysantha and accelerate the reaction process. This culture time is conducive to the full fermentation process, allowing the lactic acid bacteria to complete the transformation of the components of Camellia chrysantha.
[0034] 3. Because the phenolic hydroxyl group of 2-hydroxysalicylate can neutralize reactive oxygen species and reduce the activation of the hypothalamus-pituitary-adrenal axis by oxidative stress, and because 2-hydroxysalicylate can also indirectly reduce the excessive response of the hypothalamus-pituitary-adrenal axis by blocking the release of inflammatory factors, the polyphenols, amino acids and other active ingredients contained in Camellia chrysantha can provide a soothing environment for the skin, while 2-hydroxysalicylate, as an inhibitor, can specifically enhance the inhibitory effect on cortisol secretion. The two work synergistically to further improve skin problems caused by excessive cortisol secretion. Detailed Implementation
[0035] The present application will be further described in detail below with reference to Examples 1-7 and Comparative Examples 1-3.
[0036] raw material
[0037] The lactic acid bacteria used in this application is Lactobacillus casei SM003, which is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC NO.26537 and deposit date of February 10, 2023.
[0038] Camellia sinensis Guangxi Fangchenggang Changlong Camellia sinensis Plantation; Glucose Jinan Dehou Chemical Co., Ltd.; Proteose peptone CAS: 73049-73-7; Yeast powder Hefei Tannun Biological Technology Co., Ltd.; Diphosphoric acid potassium CAS: 7758-11-4; Magnesium sulfate Hubei Kowode Chemical Co., Ltd.; Manganese sulfate Hebei Kolonduo Biological Technology Co., Ltd.; Deionized water CAS: 7732-18-5; Potassium sorbate CAS: 24634-61-5; 2-Hydroxy salicylic acid CAS: 117354-64-0; Sodium dodecahydrododecaborate CAS: 12008-78-5; Ethanol CAS: 64-17-5.
[0039] Example 1
[0040] The embodiment provides a Camellia sinensis fermentation liquor for inhibiting cortisol secretion, and a preparation method thereof, which comprises the following steps:
[0041] S1: inoculate lactic acid bacteria into a culture medium, and incubate at 37°C under constant temperature oscillation for 24h to obtain a seed liquid;
[0042] S2: put Camellia sinensis into a pulverizer for crushing, and pass through a 40-mesh screen to obtain Camellia sinensis powder;
[0043] S3: add 1% Camellia sinensis powder, 2% glucose, 1.25% proteose peptone, 0.3% yeast powder, 0.125% diphosphoric acid potassium, 0.075% magnesium sulfate, 0.003% manganese sulfate, 0.055% inhibitor, and the rest deionized water into a fermentation tank, sterilize at 121°C for 20min, and cool to 25°C;
[0044] S4: inoculate the seed liquid into the fermentation tank according to an inoculation amount of 6.66%, and incubate at 25°C under oscillation at 200r / min for 21h to obtain a fermentation liquor;
[0045] S5: filter the fermentation liquor to remove impurities, sterilize, and add potassium sorbate to obtain the Camellia sinensis fermentation liquor;
[0046] The preparation method of the inhibitor comprises the following steps: dissolve sodium dodecahydrododecaborate and 2-hydroxy salicylic acid in an ethanol aqueous solution, stir at 25°C for 12h, filter, and freeze-dry to obtain the inhibitor;
[0047] The mass ratio of sodium dodecahydrododecaborate to 2-hydroxy salicylic acid is 1.5:1.
[0048] Examples 2-3
[0049] The difference between the embodiment and example 1 is that the fermentation conditions are different, and the specific conditions are shown in Table 1.
[0050] Table 1 Fermentation conditions in examples 1-3
[0051] Example 1 Example 2 Example 3 Inoculum (%) 6.66 7.32 6 Fermentation temperature (°C) 25 23 27 Shaking speed (r / min) 200 150 250 Culture duration (h) 21 22 20
[0052] Example 4
[0053] The difference from Example 1 is that 2-hydroxysalol is replaced by an equivalent amount of quercetin.
[0054] Examples 5-6
[0055] The difference from Example 1 is that the mass ratio of each component of the inhibitor is different, as shown in Table 2.
[0056] Table 2 Mass ratio of each component of the inhibitor in Example 1 and Examples 5-6
[0057] Example 1 Example 5 Example 6 Sodium dodecahydrododecaborate 1.5 1 2 2-hydroxy-salol 1 1 1
[0058] Example 7
[0059] The difference from Example 1 is that the mass ratio of sodium dodecahydrododecaborate to 2-hydroxysalol is 3:1.
[0060] Comparative Example 1
[0061] The difference from Example 1 is that the fermentation conditions are that the seed liquid is inoculated into the fermentation tank at an inoculation amount of 12%, and cultured at 37°C, 400r / min for 18h.
[0062] Comparative Example 2
[0063] The difference from Example 1 is that sodium dodecahydrododecaborate is no longer added.
[0064] Comparative Example 3
[0065] The difference from Example 1 is that the inhibitor is no longer added.
[0066] Performance detection test
[0067] I. Inhibition of Cortisol secretion
[0068] Three samples were taken from each of Examples 1-7 and Comparative Examples 1-3, and human immortalized keratinocytes were inoculated into 6-well plates (6x10 5
[0069] The test data are shown in Tables 3-4.
[0070] Table 3 Relative expression of 11 β-HSD1 gene of Example 1-Example 7 and Comparative Example 1-Comparative Example 3
[0071] Relative expression of 11 β-HSD1 gene Effect (%) p value Test result Example 1 0.740 26 ＜0.05 Significant Example 2 0.752 25 ＜0.05 Significant Example 3 0.760 24 ＜0.05 Significant Example 4 0.810 19 ＜0.05 Significant Example 5 0.758 24 ＜0.05 Significant Example 6 0.755 24 ＜0.05 Significant Example 7 0.850 15 ＞0.05 Not significant Comparative Example 1 0.920 8 ＞0.05 Not significant Comparative Example 2 0.880 12 ＞0.05 Not significant Comparative Example 3 0.950 5 ＞0.05 Not significant
[0072] Table 4 Relative expression of 11 β-HSD2 gene of Example 1-Example 7 and Comparative Example 1-Comparative Example 3
[0073] Relative expression of 11 β-HSD2 gene Effect (%) p value Test result Example 1 3.20 220 ＜0.01 Significant Example 2 3.15 215 ＜0.01 Significant Example 3 3.10 210 ＜0.01 Significant Example 4 2.80 180 ＜0.01 Significant Example 5 3.05 205 ＜0.01 Significant Example 6 3.00 200 ＜0.01 Significant Example 7 2.50 150 ＜0.05 Significant Comparative Example 1 1.80 80 ＞0.05 Not significant Comparative Example 2 2.00 100 ＞0.05 Not significant Comparative Example 3 1.00 0 - -
[0074] II. Cytotoxicity
[0075] Three samples were taken from Example 1-Example 7 and Comparative Example 1-Comparative Example 3, respectively, and human immortalized keratinocytes were inoculated in 96-well plates (5 x 10 3 cells / well) and incubated at 37°C, 5% CO2 for 24 h. The sample group was added with 1.25% sample, and the normal control group was replaced with fresh culture medium and incubated at 37°C, 5% CO2 for 24 h. After incubation, MTT solution was added to each well, and incubation was continued for 4 h. The culture medium was removed, DMSO solution was added, and the mixture was shaken and mixed. The absorbance value at 490 nm was measured, and the cell viability was calculated and averaged.
[0076] The test data are shown in Table 5.
[0077] Table 5 Cytotoxicity test of normal control group, Example 1-Example 7 and Comparative Example 1-Comparative Example 3
[0078]
[0079]
[0080] As can be seen from the combination of the normal control group and Example 1 and Table 5, the cell viability of Example 1 has a slight fluctuation compared with the normal control group, and there is no significant difference, and the test result shows no cytotoxicity, which indicates that the Camellia sinensis fermentation broth of Example 1 has no cytotoxicity.
[0081] It can be seen from the combination of Example 1 and Comparative Example 1 and Tables 3-5 that, compared with Example 1, the relative expression of 11β-HSD1 gene of Comparative Example 1 is significantly increased, the relative expression of 11β-HSD2 gene of Comparative Example 1 is significantly reduced, and the cell viability of Comparative Example 1 is significantly decreased and shows slight cytotoxicity compared with the normal control group, thereby indicating that the fermentation conditions affect the effect of the fermented Camellia nitidissima on the inhibition of cortisol secretion, and when the fermentation conditions are far beyond the set range, the effect of the fermented Camellia nitidissima on the inhibition of cortisol secretion is significantly reduced, and whether the fermented Camellia nitidissima has cytotoxicity is affected.
[0082] The reason is that too low inoculation amount may cause slow growth of the bacterial cells, prolong the fermentation period, and too high inoculation amount may cause nutrient competition and accumulation of metabolic by-products. Setting the fermentation temperature lower than the conventional fermentation temperature is beneficial to reducing the degradation of heat-sensitive substances such as polyphenols and flavonoids in Camellia nitidissima. The lactic acid bacteria can maintain activity in this temperature range and will not over-reproduce to cause imbalance of the system. Moderate oscillation is beneficial to providing dissolved oxygen and promoting uniform mixing of the materials, so that the lactic acid bacteria and the Camellia nitidissima components are in full contact and the reaction process is accelerated. This culture duration is beneficial to sufficient fermentation and completion of the conversion of the Camellia nitidissima components by the lactic acid bacteria.
[0083] It can be seen from the combination of Example 1 and Comparative Example 2 and Tables 3-5 that, compared with Example 1, the relative expression of 11β-HSD1 gene of Comparative Example 2 is significantly increased, the relative expression of 11β-HSD2 gene of Comparative Example 2 is significantly reduced, and the cell viability of Comparative Example 2 is decreased but shows no cytotoxicity compared with the normal control group, thereby indicating that, compared with no addition of sodium dodecahydrododecaborate, the addition of sodium dodecahydrododecaborate can effectively improve the effect of the fermented Camellia nitidissima on the inhibition of cortisol secretion.
[0084] The reason is that sodium dodecahydrododecaborate has a stable closed-cage borohydride anion structure, and its rigid cage skeleton can effectively wrap 2-hydroxychlorophenol to form a protective barrier, so that 2-hydroxychlorophenol is not easily destroyed by lactic acid bacterial metabolic enzymes or structurally degraded due to environmental factors during the fermentation process, thereby retaining the activity of inhibiting cortisol secretion.
[0085] It can be seen from the combination of Example 1 and Comparative Example 3 and Tables 3-5 that, compared with Example 1, the relative expression of 11β-HSD1 gene of Comparative Example 3 is significantly increased, the relative expression of 11β-HSD2 gene of Comparative Example 3 is significantly reduced, and the cell viability of Comparative Example 3 is decreased but shows no cytotoxicity compared with the normal control group, thereby indicating that, compared with no addition of the inhibitor, the addition of the inhibitor can effectively improve the effect of the fermented Camellia nitidissima on the inhibition of cortisol secretion.
[0086] The reason is that flavonoids can compete with 11 beta-hydroxysteroid dehydrogenase type 1, making it difficult for cortisol precursors to be converted into active cortisol, affecting the regulation process of the hypothalamic-pituitary-adrenal axis, thereby reducing the excessive secretion of cortisol, and the boron cluster-based material is beneficial to protect the flavonoids from being destroyed by the fermentation environment. The endogenous components of Camellia japonica and exogenous flavonoids work synergistically to further enhance the inhibitory effect of cortisol.
[0087] As can be seen from the combination of Example 1 and Examples 2-3 and in combination with Tables 3-5, compared with Example 1, the relative expression of 11 beta-HSD1 gene in Examples 2 and 3 is increased, and the relative expression of 11 beta-HSD2 gene in Examples 2 and 3 is reduced, and compared with the normal control group, the cell viability of Examples 2 and 3 has some fluctuations but shows no cytotoxicity, thus indicating that the fermentation conditions affect the effect of the Camellia japonica fermentation liquor on inhibiting the secretion of cortisol, and thus it can be seen that the fermentation conditions in Example 1 are optimal.
[0088] As can be seen from the combination of Example 1 and Example 4 and in combination with Tables 3-5, compared with Example 1, the relative expression of 11 beta-HSD1 gene in Example 4 is significantly increased, and the relative expression of 11 beta-HSD2 gene in Example 4 is significantly reduced, and compared with the normal control group, the cell viability of Example 4 has some fluctuations but shows no cytotoxicity, thus indicating that compared with adding quercetin, adding 2-hydroxy saclorol can effectively improve the effect of the Camellia japonica fermentation liquor on inhibiting the secretion of cortisol.
[0089] The reason is that the phenolic hydroxyl group of 2-hydroxy saclorol can neutralize active oxygen and reduce the activation of the hypothalamic-pituitary-adrenal axis caused by oxidative stress, and 2-hydroxy saclorol can also indirectly reduce the overreaction of the hypothalamic-pituitary-adrenal axis by blocking the release of inflammatory factors. The active ingredients such as polyphenols and amino acids contained in Camellia japonica can provide a soothing environment for the skin, and 2-hydroxy saclorol as an inhibitor can specifically enhance the inhibitory effect on the secretion of cortisol, and the two form a synergistic effect, which is beneficial to further improve the skin problems caused by excessive secretion of cortisol.
[0090] As can be seen from the combination of Example 1 and Examples 5-6 and in combination with Tables 3-5, compared with Example 1, the relative expression of 11 beta-HSD1 gene in Examples 5 and 6 is increased, and the relative expression of 11 beta-HSD2 gene in Examples 5 and 6 is reduced, and compared with the normal control group, the cell viability of Examples 5 and 6 has some fluctuations but shows no cytotoxicity, thus indicating that the mass ratio of the components of the inhibitor affects the effect of the Camellia japonica fermentation liquor on inhibiting the secretion of cortisol, and thus it can be seen that the mass ratio of the components of the inhibitor in Example 1 is optimal.
[0091] As can be seen in combination of Example 1 and Example 7 and in combination of Tables 3-5, the relative expression of 11β-HSD1 gene of Example 7 is significantly increased, the relative expression of 11β-HSD2 gene of Example 7 is significantly reduced, and the cell viability of Example 7 is floating but shows no cytotoxicity compared with the normal control group, thus indicating that the mass ratio of sodium dodecahydrododecaborate and 2-hydroxychlorophenol affects the effect of inhibition of cortisol secretion by Camellia sinensis fermented liquid, and thus it can be seen that the mass ratio of sodium dodecahydrododecaborate and 2-hydroxychlorophenol in Example 1 is optimal.
[0092] The reason is that when the mass ratio of sodium dodecahydrododecaborate and 2-hydroxychlorophenol is (1-2): 1, it is conducive to realizing that the rigid cage structure of sodium dodecahydrododecaborate fully wraps 2-hydroxychlorophenol to form a stable protective barrier to avoid its destruction or degradation during fermentation. This mass ratio can not only ensure that the active site of 2-hydroxychlorophenol is exposed to play a role in inhibiting cortisol secretion, but also is not prone to incomplete wrapping due to imbalance of the ratio.
[0093] The specific embodiments are only an explanation of the present application, and are not a limitation of the present application. Those skilled in the art can make modifications to the embodiments without creative contribution after reading the present specification, but as long as the modifications are within the scope of the claims of the present application, they are protected by the Patent Law.
Claims
1. A method for preparing a Camellia sinensis fermentation broth for inhibiting cortisol secretion, characterized by, The method comprises the following steps: S1: inoculate lactic acid bacteria into culture medium, and perform constant temperature oscillation culture to obtain seed liquid; S2: crush and sieve Camellia nitidissima to obtain Camellia nitidissima powder; S3: add 0.8-1.2% Camellia nitidissima powder, 0.01-0.1% inhibitor, and the rest deionized water into a fermentation tank, and perform sterilization; S4: inoculate the seed liquid into the fermentation tank, and perform constant temperature culture to obtain fermentation liquid; S5: filter and sterilize the fermentation liquid, and add preservative to obtain Camellia nitidissima fermentation liquid; The inhibitor is a boron cluster-based material wrapped flavonoids.
2. The method for preparing a fermented liquid of Camellia sinensis for inhibiting cortisol secretion according to claim 1, characterized in that: In the step S4, the seed liquid is inoculated into the fermentation tank at an inoculation amount of 6-7.32%, and is cultured at 23-27°C and 150-250r / min for 20-22h.
3. The method for preparing a Camellia chrysantha fermented liquid that inhibits cortisol secretion according to claim 1, characterized in that: The inhibitor is a boron cluster-based material wrapped 2-hydroxy-salol.
4. The method of claim 3, wherein the Camellia sinensis fermentation liquid for inhibiting cortisol secretion is prepared by the steps of: The inhibitor is a sodium dodecahydrododecaborate wrapped 2-hydroxy-salol. 5. The method for preparing a Camellia chrysantha fermented liquid that inhibits cortisol secretion according to claim 4, characterized in that, The inhibitor comprises the following raw materials: sodium dodecahydrododecaborate, 2-hydroxy-salol, and ethanol aqueous solution.
6. The method for preparing a Camellia chrysantha fermentation liquid that inhibits cortisol secretion according to claim 5, characterized in that, The preparation method of the inhibitor: dissolve sodium dodecahydrododecaborate and 2-hydroxy-salol in ethanol aqueous solution, and stir and react at 23-27°C for 10-14h, and then perform freeze-drying after filtration.
7. The method of claim 6, wherein the Camellia sinensis fermentation broth for inhibiting cortisol secretion is prepared by the steps of: The mass ratio of the sodium dodecahydrododecaborate to 2-hydroxy-salol is (1-2):
1. 8. A Camellia nitidissima fermentation liquid for inhibiting secretion of cortisol, which is prepared by the preparation method in any one of claims 1-7.
9. A cosmetic for inhibiting secretion of cortisol, which contains the Camellia nitidissima fermentation liquid in claim 8 as the main active ingredient or the only active ingredient.