Composition with relieving and itching relieving effects as well as preparation method and application thereof

Abstract

The invention discloses a composition with relieving and itching relieving effects as well as a preparation method and application thereof, and particularly belongs to the field of cosmetics. The composition comprises the following components: a composite plant extract, polyol and water, the composite plant extract is prepared by compounding a rhizoma polygonati fermentation liquid, a Chinese herbaceous peony extract and a caulerpa lentillifera extract; the rhizoma polygonati fermentation liquor is obtained by carrying out compound fermentation on lactobacillus pentosus and bacillus subtilis. The soothing and itching relieving composition provided by the invention has an excellent soothing effect, the soothing and itching relieving composition provided by the invention has an excellent in-vitro hyaluronidase inhibition effect, and the in-vitro hyaluronidase inhibition rate reaches 60% or above.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of cosmetics, and particularly relates to a composition with soothing and antipruritic effects and a preparation method and application thereof. BACKGROUND

[0002] Skin itching is a common symptom of various skin diseases (such as eczema, dermatitis, urticaria, etc.) and allergic reactions, which seriously affects the quality of life of patients. At present, chemical synthetic ingredients such as glucocorticoids and antihistamines are often used in clinical treatment, which can quickly relieve symptoms, but long-term use can easily cause side effects such as skin atrophy, capillary dilation, drug resistance, and cannot solve the problems of deep inflammation and skin barrier repair. In recent years, natural plant extracts have gradually attracted attention due to their high safety and small side effects, such as glycyrrhizic acid, aloe polysaccharide, and purslane extract, which have been applied in soothing products. However, the antipruritic effect of single active ingredient in the prior art is limited, and when multiple ingredients are compounded, there are often problems such as insufficient synergistic effect, poor stability, and low transdermal absorption efficiency. In addition, traditional preparation processes often use high temperature or organic solvent extraction, which can easily cause inactivation of heat-sensitive active ingredients and reduce product efficacy; the dosage form design is also relatively single, and it is difficult to meet the dual needs of rapid antipruritic and long-term repair.

[0003] Therefore, developing a composition with multiple active ingredients synergistically, a mild and efficient preparation process, and both instant soothing and long-term repair functions has become an important direction to solve the defects of the prior art. SUMMARY

[0004] In view of the deficiencies of the prior art, the purpose of the present application is to provide a composition with synergistic effect of multiple active ingredients, which is prepared by fermentation process combined with traditional solvent extraction process, and has the effects of soothing and repairing, anti-allergic and antipruritic.

[0005] In order to achieve the above-mentioned purpose, the following technical solutions are disclosed: In a first aspect, the present application provides a composition with soothing and antipruritic effects, comprising the following components by mass percentage: Compound plant extract 13-15%; Polyhydric alcohol 3-8%; Water, supplemented to 100%; The compound plant extract is compounded by the fermentation liquor of Rhizoma Polygonati, the extract of Paeonia lactiflora Pall, and the extract of Caudimargo Gracilis; The preparation method of the fermentation liquor of Rhizoma Polygonati includes the following steps: Step 1-1. The Rhizoma Polygonati is pulverized and sieved to obtain Rhizoma Polygonati powder; Step 1-2. The Rhizoma Polygonati powder is mixed with water at a solid-liquid ratio of 1: (10-15) g / mL to obtain a fermentation substrate; Step 1-3. The complex bacteria solution of Lactobacillus pentosus and Bacillus subtilis is inoculated into the fermentation medium at an inoculation amount of 8-10 v / v%, the fermentation temperature is 30-37℃, the stirring speed is 100-150 r / min, the aeration amount is 0.5-1vvm, and the fermentation culture is carried out for 2-3 days. After the fermentation is completed, sterilization is carried out at 121℃ for 20 min, and centrifugation is carried out after cooling to obtain the fermented liquor of Rhizoma Polygonati.

[0006] Preferably, the viable cell count of Lactobacillus pentosus in the complex bacteria solution is (1-2)×10 8 CFU / mL, and the viable cell count of Bacillus subtilis is (5-6)×10 8 CFU / mL.

[0007] Further preferably, the preparation method of the extract of Paeonia lactiflora Pall. comprises the following steps: Step 2-1. A certain amount of Paeonia lactiflora Pall. is crushed and then soaked in water, the soaking time is 12-16h, the soaking temperature is 25-35℃, and the solid-liquid ratio is 1: (10-15) g / mL. After the soaking is completed, centrifugal separation is carried out to obtain filtrate 1 and residue; Step 2-2. The residue is added with 70 v / v% ethanol aqueous solution, the solid-liquid ratio is 1: (20-25) g / mL, extraction is carried out in a water bath at 120-140℃ for 120-140 min, and after the extraction is completed, centrifugal separation is carried out to obtain filtrate 2. The filtrate 2 is combined with the filtrate 1, and vacuum concentration and drying are carried out to obtain the extract of Paeonia lactiflora Pall.

[0008] Still further preferably, the preparation method of the extract of Acanthopanax giraldii Harms comprises the following steps: Step 3-1. The dried Acanthopanax giraldii Harms is crushed and sieved to obtain Acanthopanax giraldii Harms powder for use; Step 3-2. The Acanthopanax giraldii Harms powder is mixed with 75 v / v% ethanol aqueous solution at a solid-liquid ratio of 1: (30-45) g / mL, extraction is carried out in a water bath at 60-70℃ for 100-120 min, centrifugal separation is carried out after the extraction is completed, the residue is removed, and vacuum concentration and drying are carried out to obtain the extract of Acanthopanax giraldii Harms.

[0009] Still further preferably, the complex plant extract is prepared by compounding the fermented liquor of Rhizoma Polygonati, the extract of Paeonia lactiflora Pall. and the extract of Acanthopanax giraldii Harms at a mass ratio of 10: (1-4) :1.

[0010] Preferably, the polyol is at least one of glycerol, 1,3-propanediol and 1,2-hexanediol.

[0011] In the second aspect, the application provides the use of the composition with soothing and antipruritic effects in the preparation of a skin care product with soothing, antipruritic and / or repairing effects.

[0012] In a third aspect, the present application provides a skin resident agent with soothing and anti-itching effects, comprising the composition with soothing and anti-itching effects according to the first aspect. The composition with soothing and anti-itching effects is added in the skin resident agent in an amount of 5-10 wt%.

[0013] Preferably, the composition further comprises a moisturizer, an emollient, a preservative, a pH regulator, an emulsifier, and a solvent.

[0014] In the present application, After the Huangjing is fermented by the Lactobacillus pentosus and Bacillus subtilis, the active ingredients (such as polysaccharides, saponins, flavonoids) are degraded into small molecule oligosaccharides, short chain fatty acids and isoflavone aglycone, etc., which significantly improve the transdermal absorption efficiency, inhibit the release of inflammatory factors (such as TNF-α), regulate the Th1 / Th2 immune balance, and block the histamine signal transmission, and have the effects of anti-inflammation, anti-itching and anti-allergy; at the same time, a small amount of organic acid (lactic acid, acetic acid) in the fermentation product can repair the skin barrier and enhance the moisturizing property. The Huangjing fermentation broth prepared by the fermentation process avoids the damage of high temperature or organic solvent to part of the active ingredients, so that the product has both mildness and high efficiency.

[0015] The Paeonia extract contains rich paeoniflorin, flavonoids and polysaccharides, etc., and has the effects of soothing, anti-itching and anti-allergy: the paeoniflorin can inhibit the release of inflammatory mediators (such as TNF-α), reduce the histamine sensitivity, and relieve redness, swelling and itching; the flavonoid components can scavenge free radicals and reduce oxidative stress damage, and the tannin can converge and calm the red skin; at the same time, the polysaccharide substance can strengthen the skin barrier function and reduce the penetration of external stimuli.

[0016] The Cystoseira osmundacea extract contains rich polyphenol compound ingredients, and has the effects of soothing, anti-itching and anti-allergy. The polyphenol compound can scavenge free radicals and inhibit the release of histamine, and relieve the itching and sensitive reaction caused by external stimuli (such as ultraviolet rays and pollutants).

[0017] The present application has the following advantages: 1. The soothing and anti-itching composition provided by the present application has excellent soothing effect. Compared with the negative control group, the content of TNF-α inflammatory factor in the macrophages treated by the soothing and anti-itching composition containing the Huangjing fermentation broth, the Paeonia extract and the Cystoseira osmundacea extract is significantly reduced, and the TNF-α inhibition rate reaches 38.26%, 41.46% and 40.29%, respectively. 2. The soothing and anti-itching composition provided by the present application has a significantly improved hyaluronidase inhibition rate compared with the composition lacking any one of the components. The in vitro hyaluronidase inhibition rate reaches more than 60%, which indicates that the Huangjing fermentation broth, the Paeonia extract and the Cystoseira osmundacea extract can promote each other when used in combination, so that the composition has a significant soothing, anti-allergic and anti-itching effect.

[0018] 3.In the present application, the Polygonatum rhizome fermentation broth is prepared by fermentation with the complex bacteria consisting of Lactobacillus pentosus and Bacillus subtilis. The soothing and antihistamine effect of the soothing and antihistamine composition prepared using the fermentation broth is significantly improved compared with the composition prepared using the Polygonatum rhizome water extract or the Polygonatum rhizome fermentation broth produced by single strain fermentation, indicating that the two functional bacteria in the complex bacteria used in the present application can interact and promote each other during mixed fermentation, so that the product has both mildness and high efficiency. DETAILED DESCRIPTION

[0019] To better illustrate the object, technical scheme and advantages of the present application, the present application will be further described below in combination with specific examples. Those skilled in the art should understand that the specific examples described herein are only used to explain the present application and do not limit the present application.

[0020] The test methods used in the examples and comparative examples are conventional methods unless otherwise specified; the materials, reagents, etc. used are commercially available unless otherwise specified; the percentages mentioned in the examples and comparative examples are mass percentages unless otherwise specified.

[0021] In the present application:

[0022] Polygonatum rhizome: from the self-owned planting base of Shanghai Jingxin Biotechnology Co., Ltd.; Paeonia suffruticosa: from the self-owned planting base of Shanghai Jingxin Biotechnology Co., Ltd.; Long-stemmed grape fern: purchased from Shenzhen Lanting Dingzhi Biological Technology Co., Ltd.; Lactobacillus pentosus: purchased from China General Microbiological Culture Collection Center, with the preservation number of CGMCC 1.2707; Bacillus subtilis: purchased from China General Microbiological Culture Collection Center, with the preservation number of CGMCC NO.1.821.

[0023] Preparation of Polygonatum rhizome fermentation broth

[0024] The preparation method of the Polygonatum rhizome fermentation broth 1 includes the following steps: Step 1-1. Dry the Polygonatum rhizome root slices to a constant weight, crush them in a crusher, and pass them through a 30-mesh sieve to obtain Polygonatum rhizome powder; Step 1-2. Mix the Polygonatum rhizome powder obtained in step 1-1 with water at a material-to-liquid ratio of 1:13 g / mL to obtain a fermentation substrate, and sterilize it at 121℃ for 20 min for standby; Step 1-3. The complex bacteria solution of Lactobacillus pentosus and Bacillus subtilis was inoculated into the fermentation medium at a 10 v / v% inoculation amount, the fermentation temperature was 37℃, the stirring speed was 150 r / min, the aeration amount was 0.8 vvm, and the fermentation culture was carried out for 3 days. After the fermentation was completed, sterilization was carried out at 121℃ for 20 min, and centrifugation was carried out after cooling to obtain the Huangjing fermentation liquor 1, wherein the viable cell count of Lactobacillus pentosus in the complex bacteria solution was 1×10 8 CFU / mL, and the viable cell count of Bacillus subtilis was 5×10 8 CFU / mL.

[0025] Huangjing fermentation liquor 2: Compared with the preparation method of the Huangjing fermentation liquor 1, the only difference is that the complex bacteria solution inoculated in step 1-3 is replaced by Lactobacillus pentosus solution alone, and the viable cell count and the rest of the conditions and parameters are consistent with the preparation method of the Huangjing fermentation liquor 1.

[0026] Huangjing fermentation liquor 3: Compared with the preparation method of the Huangjing fermentation liquor 1, the only difference is that the complex bacteria solution inoculated in step 1-3 is replaced by Bacillus subtilis solution alone, and the viable cell count and the rest of the conditions and parameters are consistent with the preparation method of the Huangjing fermentation liquor 1.

[0027] Huangjing water extract: The slices of Huangjing roots were dried to constant weight, crushed in a crusher, and sieved through a 30-mesh sieve to obtain Huangjing powder. The Huangjing powder was mixed with deionized water at a solid-liquid ratio of 1:20 g / mL, soaked at 35℃ for 12 h, and then centrifuged to separate the residue and obtain the Huangjing water extract.

[0028] Preparation of peony extract

[0029] Step 2-1. The peony was dried to constant weight, crushed in a crusher, and sieved through a 30-mesh sieve to obtain peony powder. The peony powder was soaked in water, the soaking time was 14 h, the soaking temperature was 30℃, the solid-liquid ratio was 1:15 g / mL, and after soaking, centrifugal separation was performed to obtain filtrate 1 and residue; Step 2-2. The residue was added with 70 v / v% ethanol aqueous solution at a solid-liquid ratio of 1:25 g / mL, and extracted in a 65℃ water bath for 140 min. After extraction, centrifugal separation was performed to obtain filtrate 2. The filtrate 2 and the filtrate 1 were combined, and concentrated under reduced pressure to dryness to obtain a peony extract with a water content of 23-25%.

[0030] Preparation of long-stem grape caulerpa extract

[0031] Step 3-1. The dried long-stem grape caulerpa was crushed and sieved through a 60-mesh sieve to obtain long-stem grape caulerpa powder for use; Step 3-2. The long-stem grape caulerpa powder was mixed with 75 v / v% ethanol aqueous solution at a solid-liquid ratio of 1:40 g / mL, and extracted in a 70℃ water bath for 100 min. After centrifugal separation to remove the residue and retain the liquid, the liquid was concentrated under reduced pressure to dryness to obtain a long-stem grape caulerpa extract with a water content of 8-10%.

[0032] Preparation of composite plant extract

[0033] The composite plant extract is compounded by Huangjing fermentation liquor, peony extract and long-stemmed grape caulerpa extract at a mass ratio of 10: (1-4): 1, and the specific formulation is shown in Table 1.

[0034] Table 1 Raw material ratio of composite plant extract Raw materials Composite plant extract 1 Composite plant extract 2 Composite plant extract 3 Composite plant extract 4 Composite plant extract 5 Composite plant extract 6 Composite plant extract 7 Composite plant extract 8 Rhizoma polygonati fermentation broth 1 10 10 10 10 10 - Paeonia extract 1 3 4 - 3 3 3 3 Caulis adianti extract 1 1 1 1 - 1 1 1 Rhizoma polygonati water extract - - - - - 10 - - Rhizoma polygonati fermentation broth 2 - - - - - - 10 - Rhizoma polygonati fermentation broth 3 - - - - - - - 10 Note: "-" in the table indicates no addition of the corresponding component, and the missing amount is distributed proportionally to the remaining components; and the total amount of the composite plant extract is the same.

[0035] Preparation of soothing and itching-relieving composition

[0036] The raw materials are accurately weighed according to the mass percentage in Table 2, and placed in a high-speed homogenizer for homogenization and dispersion for 15 minutes to obtain the soothing and itching-relieving composition.

[0037] Table 2 Mass percentage of raw materials of soothing and itching-relieving composition Raw materials Composition 1 Composition 2 Composition 3 Composition 4 Composition 5 Composition 6 Composition 7 Composition 8 Composite plant extract / % 13 14 15 14 14 14 14 14 Composite plant extract serial number Extract 1 Extract 2 Extract 3 Extract 4 Extract 5 Extract 6 Extract 7 Extract 8 Glycerin / % 3 5 5 5 5 5 5 5 1,3-propanediol / % - - 1 - - - - - 1,2-hexanediol / % - - 2 - - - - - Deionized water / % Supplemented to 100 Supplemented to 100 Supplemented to 100 Supplemented to 100 Supplemented to 100 Supplemented to 100 Supplemented to 100 Supplemented to 100 Note: "-" in the table indicates no addition.

[0038] Performance test:

[0039] To verify the soothing and itching-relieving effect of the composite plant extract, and to verify the relationship between the components in the composite plant extract, the composite plant extract 2 formula is used as the basis to make default or replacement, and the composite plant extracts 4-8 are obtained, as shown in Table 1. The composite plant extracts 1-8 are prepared into soothing and itching-relieving compositions 1-8, and the following performance tests are carried out.

[0040] 1. Soothing effect test

[0041] (1) Test basis: This experiment is designed and tested according to the standard of "T / SHRH034-2021 Cosmetic soothing effect test - In vitro TNF-α inflammatory factor content determination of lipopolysaccharide-induced macrophage cell line inflammatory cell model test method".

[0042] (2) Test principle: LPS-induced RAW264.7 macrophages are a classic cell model for studying inflammatory factors. By comparing the difference in TNF-α content secreted by RAW264.7 after administration of negative control and test sample, the ability of the test sample to inhibit the secretion of cell inflammatory factors is evaluated, and the soothing effect of the sample to be tested is explored.

[0043] (3) Test cells: Mouse monocyte macrophage leukemia cell line RAW264.7 is selected, and the cell line has passed the cell line quality control experiment detection, and the results are qualified. The test generation number is P20.

[0044] (4) Main reagents: fetal bovine serum, high-sugar DMEM medium, penicillin-streptomycin solution, trypsin-EDTA solution, TNF-α detection kit, lipopolysaccharide, dexamethasone sodium phosphate (DSP).

[0045] (5) Test method ① Grouping and control Product dose grouping: The sample group to be tested is the above-prepared compositions 1-8, which are diluted to 1% sample solution with cell culture solution (MTT cytotoxicity test is performed first to determine the safe working concentration).

[0046] Test control information: ① Blank control: complete culture medium; ② Negative control: 0.5 μg / mL LPS stimulation; ③ Positive control: 0.5 μg / mL LPS + 100 μg / mL dexamethasone sodium phosphate (DSP).

[0047] ② Operation steps 1) Cell preparation: After stable passage twice from the frozen cell culture, inoculate the 96-well plate and place the 96-well plate in a 5v / v% CO2 incubator for overnight culture.

[0048] 2) Drug administration: discard the culture medium in the 96-well plate and perform drug administration. The blank control group is given complete culture medium, the negative control group is given cell culture medium containing 0.5 μg / mL LPS, and the remaining groups are given cell culture medium containing 0.5 μg / mL LPS + corresponding concentration of test substance, with 6 replicate wells in each group. After drug administration, place the 96-well plate in a CO2 incubator for 24 h.

[0049] 3) Inflammatory factor detection: after incubation, detect according to the instructions of the TNF-α enzyme-linked immunosorbent kit, and measure the absorbance value of each sample well at 450 nm with an enzyme marker.

[0050] (6) Experimental results Inhibition rate (%) = (A0-A1) / A0×100% Where: A0: mean TNF-α content of the negative control group; A1: mean TNF-α content of the test substance / positive control group.

[0051] Table 3 TNF-α inflammatory factor content and inhibition rate in macrophages

[0052] Note: "a" means significant difference compared with the negative control group, "aa": p<0.01, "aaa": p<0.001; "b" means significant difference compared with the sample group 2, "bb": p<0.01, "bbb": p<0.001.

[0053] The content of TNF-α inflammatory factor secreted by macrophages and the inhibition rate of sample groups 1-8 under LPS stimulation culture conditions are shown in Table 3. Compared with the negative control group, the content of TNF-α inflammatory factor in macrophages treated by sample groups 1-3 containing the fermented liquid of Polygonatum, the extract of Paeonia and the extract of Caudimargo Gracilis Caulerpa was significantly reduced, and the inhibition rates of TNF-α were 38.26%, 41.46% and 40.29% respectively, indicating that the compound plant extracts 1-3 had significant ability to inhibit the secretion of TNF-α inflammatory factor. Compared with sample group 2, the anti-inflammatory performance of sample groups 4-8 decreased significantly, among which the inhibition rates of sample groups 4 and 5 were significantly different, indicating that the fermented liquid of Polygonatum, the extract of Paeonia and the extract of Caudimargo Gracilis Caulerpa played a key role in the formula, and the absence of any component led to a significant decrease in performance; the replacement of the fermented liquid 1 of Polygonatum in sample groups 6-8 with the water extract of Polygonatum, the fermented liquid 2 of Polygonatum and the fermented liquid 3 of Polygonatum led to a certain degree of decrease in anti-inflammatory performance, indicating that the active ingredients in the fermented liquid increased after the compound fermentation of Lactobacillus pentosus and Bacillus subtilis, making the composition have more excellent soothing effect.

[0054] 2. Anti-allergic effect test - hyaluronidase inhibition test

[0055] Hyaluronidase is a specific lytic enzyme of hyaluronic acid, which plays an important inhibitory role in many development and regulation processes in the human body such as cell adhesion, organ formation, wound healing, tumor occurrence and blood vessel formation. Inhibiting the activity of hyaluronidase can decompose hyaluronic acid and maintain normal physiological function. Hyaluronidase is a participant in type I allergic reaction, and most allergic reactions are type I allergic reactions related to the activity of hyaluronidase in the body. The inhibition ability of the sample on hyaluronidase reflects its ability to inhibit type I allergic reaction, and the greater the inhibition rate of hyaluronidase, the stronger the anti-allergic activity.

[0056] 1. Test materials: Take the compositions 1-8 as the sample solution; 500 U / mL hyaluronidase solution; pH = 5.4 acetic acid buffer; 0.5 mg / mL sodium hyaluronate solution; acetylacetone solution (3.5 mL acetylacetone is dissolved in 50 mL 0.1 mol / L sodium carbonate solution); P-DAB color developing agent (0.8 g p-dimethylaminobenzaldehyde is uniformly mixed with 15 mL concentrated HCl and anhydrous ethanol); all are prepared fresh.

[0057] 2. Test method: according to the Elson-Morgan method, 0.5 ml of the sample solution to be tested and 0.5 ml of the hyaluronidase solution are added to tube No. 1; 0.5 ml of the sample solution to be tested and 0.5 ml of the acetic acid buffer solution are added to tube No. 2; 0.5 ml of distilled water and 0.5 ml of the hyaluronidase solution are added to tube No. 3; 0.5 ml of distilled water and 0.5 ml of the acetic acid buffer solution are added to tube No. 4, and incubated at 37°C for 20 min; 0.5 mL of the sodium hyaluronate solution is added to tubes No. 1 and No. 3, and 0.5 ml of the acetic acid buffer solution is added to tubes No. 2 and No. 4, and incubated at 37°C for 40 min; after being left to stand at room temperature for 10 min, 0.5 mL of the acetylacetone solution, 0.1 mL of the NaOH solution (5 mol / L) and 0.5 mL of distilled water are added, and immediately after being heated in a boiling water bath for 15 min, the solution is cooled with ice water for 10 min; after being left to stand at room temperature for 10 min, 1 mL of the P-DAB color developing agent is added; after being shaken thoroughly, 3.5 mL of anhydrous ethanol is added, and left to stand at room temperature for 30 min, and then colored; the absorbance A1, A2, A3 and A4 of the solutions in tubes No. 1, No. 2, No. 3 and No. 4, respectively, is measured at 530 nm, and the inhibition rate of the hyaluronidase is calculated according to the following formula.

[0058] Hyaluronidase inhibition rate = [(A3-A4)-(A1-A2)] / (A3-A4) x 100% In the formula, A1, A2, A3 and A4 are the OD values of the solutions in tubes No. 1, No. 2, No. 3 and No. 4, respectively.

[0059] 3. Test results: The results of the inhibition rate of the hyaluronidase are shown in Table 4.

[0060] Table 4 Inhibition rate of hyaluronidase Group Hyaluronidase inhibition rate / % Composition 1 61.3 Composition 2 73.4 Composition 3 70.8 Composition 4 42.8 Composition 5 39.6 Composition 6 50.1 Composition 7 52.7 Composition 8 58.2 According to the test results, the compositions 1-3 have excellent in-vitro hyaluronidase inhibition efficacy, reaching 61.3%, 73.4% and 70.8%, respectively. The hyaluronidase inhibition rate of the compositions 4-8 is significantly decreased compared to that of the composition 2, indicating that the Huangjing fermentation liquor, the Paeonia extract and the long-stemmed grape caulerpa extract have better soothing, anti-allergic and itching-relieving effects when used in combination.

[0061] Finally, it should be noted that the above description is only a preferred embodiment of the present application and is not intended to limit the present application. Although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions recorded in the foregoing embodiments or make equivalent replacements to some technical features. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A composition having soothing antipruritic efficacy, characterized in that, By mass percent, comprising the following components: Compound plant extract 13-15%; Polyhydric alcohol 3-8%; Water supplement to 100%; The compound plant extract is compounded by Huangjing fermentation liquor, Peony extract and Caulescent grape extract; The preparation method of the Huangjing fermentation liquor comprises the following steps: Step 1-1. Huangjing is pulverized and sieved to obtain Huangjing powder; Step 1-2. Huangjing powder is mixed with water at a material-liquid ratio of 1: (10-15) g / mL to obtain a fermentation substrate; Step 1-3. Compound bacteria liquor of Lactobacillus pentosus and Bacillus subtilis is taken at an inoculation amount of 8-10 v / v%, added to the fermentation substrate, the fermentation temperature is 30-37℃, the stirring speed is 100-150 r / min, the aeration amount is 0.5-1vvm, and the fermentation culture is carried out for 2-3 days, after the fermentation is completed, sterilization is carried out at 121℃ for 20 min, and centrifugation is carried out after cooling to obtain Huangjing fermentation liquor.

2. The composition for soothing and relieving itching according to claim 1, wherein The viable cell number of Lactobacillus pentosus in the complex bacterial liquid is (1-2) x 10 8 CFU / mL, and the viable cell number of Bacillus subtilis is (5-6) x 10 8 CFU / mL.

3. The composition for soothing and relieving itching according to claim 2, wherein The preparation method of the Peony extract comprises the following steps: Step 2-1. A certain amount of Peony is pulverized and then soaked in water, the soaking time is 12-16h, the soaking temperature is 25-35℃, the material-water ratio is 1: (10-15) g / mL, after the soaking is completed, centrifugal separation is carried out to obtain filtrate 1 and residue; Step 2-2. The residue is added with 70v / v% ethanol aqueous solution, the material-liquid ratio is 1: (20-25) g / mL, extraction is carried out in a 60-70℃ water bath for 120-140min, after the extraction is completed, centrifugal separation is carried out to obtain filtrate 2, the filtrate 2 is combined with the filtrate 1, and drying is carried out under reduced pressure to obtain Peony extract.

4. The composition for soothing and relieving itching according to claim 3, wherein The preparation method of the Caulescent grape extract comprises the following steps: Step 3-1. Dry Caulescent grape is pulverized and sieved to obtain Caulescent grape powder for use; Step 3-2. Caulescent grape powder is mixed with 75v / v% ethanol aqueous solution at a material-liquid ratio of 1: (30-45) g / mL, extraction is carried out in a 60-70℃ water bath for 100-120min, after the extraction is completed, centrifugal separation is carried out, the residue is removed, and drying is carried out under reduced pressure to obtain Caulescent grape extract.

5. The composition for soothing and relieving itching according to claim 4, wherein The compound plant extract is compounded by Huangjing fermentation liquor, Peony extract and Caulescent grape extract at a mass ratio of 10: (1-4) :

1.

6. The composition for soothing and relieving itching according to claim 1, wherein The polyhydric alcohol is at least one of glycerol, 1,3-propanediol and 1,2-hexanediol.

7. Use of the composition with soothing and antipruritic effects according to any one of claims 1-6 in the preparation of a skin care product with soothing, anti-sensitivity and / or repairing effects.

8. A skin-resident agent having soothing antipruritic efficacy, characterized in that, The composition with soothing and antipruritic effects according to any one of claims 1-6.

9. The skin resident agent having soothing and antipruritic efficacy according to claim 8, wherein Further comprising components: humectants, emollients, preservatives, pH adjusters, emulsifiers, solvents.

10. The skin resident agent having soothing and antipruritic efficacy according to claim 9, wherein The addition amount of the composition with soothing and antipruritic effects in the skin resident agent is 5-10wt%.

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