Composition containing pearl extract, pearl cream and preparation method of pearl cream

Abstract

The invention discloses a pearl extract-containing composition, pearl cream and a preparation method thereof, and belongs to the technical field of cosmetics, the pearl extract-containing composition comprises a pearl extract, an echinacea angustifolia leaf extract and an artemisia capillaris extract; the mass ratio of the pearl extract to the echinacea angustifolia leaf extract to the artemisia capillaris extract is (0.1-0.6): (0.1-0.5): (0.1-1). Under the synergistic effect of the pearl extract, the echinacea angustifolia leaf extract and the artemisia capillaris extract, the pearl cream with excellent moisturizing, anti-oxidation and soothing effects is obtained, and the pearl cream has wide application prospects.

Description

TECHNICAL FIELD

[0001] The present application relates to the cosmetic technology field, and in particular to a pearl extract-containing composition, a pearl cream and a preparation method thereof. BACKGROUND

[0002] With the acceleration of the pace of life and the aggravation of environmental pollution, skin problems are increasingly prominent, especially sensitive skin, which is more susceptible to external environmental factors such as ultraviolet rays, infrared rays, environmental pollution, and internal factors such as diet, alcohol, stress, and sleep problems. Internal and external factors can induce skin oxidative stress through active oxygen free radicals, active nitrogen free radicals, and carbonyl free radicals, leading to cell damage, DNA mutation, and thus a series of problems such as skin aging and sensitivity.

[0003] Using skin care products with soothing and antioxidant effects can help protect the skin barrier from external and internal factors.

[0004] In view of this, the present application is proposed. SUMMARY

[0005] The present application aims to overcome the deficiencies in the prior art and provides a pearl extract-containing composition, a pearl cream and a preparation method thereof, which have excellent moisturizing, antioxidant and soothing effects.

[0006] To achieve the above-mentioned purpose, the technical solution adopted by the present application is as follows:

[0007] A pearl extract-containing composition, comprising pearl extract, chamaebatia folium extract and artemisia capillaris extract;

[0008] The mass ratio of the pearl extract, chamaebatia folium extract and artemisia capillaris extract is (0.1-0.6):(0.1-0.5):(0.1-1).

[0009] As a preferred embodiment of the present application, the preparation method of the pearl extract comprises the following steps:

[0010] (1) adding pearl powder to water, ultrasonic treatment, then adding papain, neutral protease and beta-glucanase for enzymolysis to obtain an enzymolysis solution;

[0011] (2) sequentially ultrafiltering the enzymolysis solution with an ultrafiltration membrane with a molecular weight cut-off of 1000 Da and an ultrafiltration membrane with a molecular weight cut-off of 10000 Da to obtain the pearl extract.

[0012] As a preferred embodiment of the present application, the power of the ultrasonic treatment is 500-800 W, and the time of the ultrasonic treatment is 25-40 min.

[0013] As a preferred embodiment of the present application, the mass ratio of the pearl powder, water, papain, neutral protease, and beta-glucanase is 1:(4-10):(0.01-0.03):(0.005-0.02):(0.005-0.02).

[0014] As a preferred embodiment of the present application, the enzymolysis temperature is 48-55°C, and the enzymolysis time is 1-5h.

[0015] As a preferred embodiment of the present application, the preparation method of the Artemisia capillaris extract comprises the following steps:

[0016] (1) performing supercritical extraction on the Artemisia capillaris powder to obtain an extraction liquid;

[0017] (2) adding the extraction liquid, an enzymolysis promoter, and a composite enzyme into water, performing enzymolysis to obtain an enzymolysis liquid;

[0018] (3) adjusting the pH of the enzymolysis liquid to 6.2-6.8, passing the enzymolysis liquid through a macroporous adsorption resin at a flow rate of 1-2 BV / h, and eluting at a speed of 0.5-2 BV / h, first washing 1-2 BV of water, then washing 1-2 BV of 60-85 wt% ethanol solution, collecting the ethanol solution eluate, and drying to obtain the Artemisia capillaris extract.

[0019] As a preferred embodiment of the present application, the supercritical extraction uses CO2 as the extractant, 75-95 wt% ethanol as the entrainer, the CO2 flow rate is 60-120 mL / min, the extraction temperature is 38-45°C, the extraction pressure is 28-32 MPa, and the extraction time is 50-80 min.

[0020] As a preferred embodiment of the present application, the enzymolysis promoter comprises at least one of sodium carbonate and sodium bicarbonate; and / or

[0021] The composite enzyme comprises cellulase, xylanase, and beta-glucanase, and the mass ratio of the cellulase, xylanase, and beta-glucanase is 1:(0.4-0.8):(0.4-0.8).

[0022] As a preferred embodiment of the present application, the mass ratio of the extraction liquid, the enzymolysis promoter, the composite enzyme, and water is 1:(0.005-0.02):(0.02-0.04):(4-10).

[0023] As a preferred embodiment of the present application, the enzymolysis temperature is 48-55°C, and the enzymolysis time is 1-5h.

[0024] As a preferred embodiment of the present application, the mass ratio of the total mass of the pearl extract and the leaf extract of Chia to the mass of the Artemisia capillaris extract is 1:(0.5-3).

[0025] The present application also provides a pearl cream, comprising the following components in parts by mass: 0.3-2.1 parts of the pearl extract-containing composition, 0.05-0.2 parts of the tremella fruiting body extract, 0.05-0.2 parts of glyceryl glucoside, 0.2-0.6 parts of tocopheryl acetate, 0.2-0.6 parts of betaine, 0.2-0.6 parts of thickening agent, 0.5-2 parts of methylpropanediol, 2-3.5 parts of emulsifier, 1-4 parts of butanediol, 4-8 parts of glycerol, 4-10 parts of emollient, 70-85 parts of water.

[0026] The pearl extract-containing composition is the pearl extract-containing composition described above.

[0027] As a preferred embodiment of the present application, the thickening agent comprises at least one of xanthan gum, hydrolyzed micrococcus gum, hydroxyethyl cellulose, carbomer, carrageenan, guar gum, locust bean gum, carboxymethyl cellulose, and sodium polyacrylate.

[0028] As a preferred embodiment of the present application, the emollient comprises at least one of squalane, shea butter, ethylhexyl palmitate, caprylic / capric triglyceride, jojoba oil, isononyl isononanoate, isododecane, isohexadecane, octyldodecanol, dicaprylyl carbonate, and macadamia integrifolia oil.

[0029] As a preferred embodiment of the present application, the emulsifier comprises at least one of glyceryl stearate, PEG-100 stearate, sorbitan olivate, PEG-30 soya sterol, polyglyceryl-10 stearate, polyglyceryl-10 myristate, polyglyceryl-5 trioleate, PEG-40 hydrogenated castor oil, PEG-60 hydrogenated castor oil, PPG-13-decyltetradecin polyether-24, nonylphenol polyether-12, stearyl alcohol polyether-2, stearyl alcohol polyether-21, and hydrogenated lecithin.

[0030] The present application also provides a preparation method of the pearl cream, comprising the following steps:

[0031] Mixing methylpropanediol, butanediol, glycerol, thickening agent, and water uniformly, homogenizing to obtain an aqueous phase;

[0032] Heating the emulsifier and emollient to 80-85℃, mixing uniformly to obtain an oil phase;

[0033] Adding the oil phase to the aqueous phase, stirring uniformly at 80-85℃, homogenizing, cooling to 40-45℃, adding the remaining materials, stirring uniformly, homogenizing to obtain the pearl cream.

[0034] The composition has excellent moisturizing, antioxidant and soothing effects, can absorb moisture in the air and lock in moisture, reduce transdermal water loss, effectively nourish the skin, promote the absorption and moisturization of the skin, effectively repair the skin barrier, relieve the discomfort of the skin caused by external stimuli (such as ultraviolet rays, pollutants, etc.), reduce the tightness and swelling of the skin, effectively reduce the swelling, pain and other symptoms of the skin caused by inflammation, effectively inhibit the growth and reproduction of bacteria, thereby reducing skin problems caused by bacterial infection, relieving skin redness, itching, inflammation and other phenomena, inhibiting histamine release and stabilizing mast cell membranes, reducing skin allergy problems, effectively removing free radicals, reducing the damage of oxidative stress to the skin, and having a wide application prospect. DETAILED DESCRIPTION

[0035] To make the objectives, technical solutions, and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be described clearly and completely below. Obviously, the described embodiments are only some, but not all of the embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by a person of ordinary skill in the art without creative work fall within the protection scope of the present application.

[0036] In the present application, the technical features described in an open manner include both a closed technical solution consisting of listed features and an open technical solution containing listed features.

[0037] In the present application, if no special description is provided, the numerical range is regarded as continuous and includes the minimum value and the maximum value of the range and each value between the minimum value and the maximum value. Further, when a range is referred to as an integer, each integer between the minimum value and the maximum value of the range is included. In addition, when multiple ranges are provided to describe a feature or a characteristic, the ranges can be combined. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all sub-ranges subsumed therein.

[0038] In the present application, the specific dispersion and stirring treatment methods are not particularly limited.

[0039] Unless otherwise specified, the components and raw materials used in the embodiments and comparative examples of the present application are commercially available raw materials or instruments, and the components and raw materials used in each parallel experiment are the same.

[0040] The leaf extract of Chamaebatia folgneri is included in the Catalogue of Cosmetics Raw Materials (2021 Edition) with the number 06938 and is derived from Lipotec S.A.U.

[0041] The Tremella fruiting body extract is from Shanghai Boshi Industry.

[0042] The Portulaca oleracea extract is from Guangzhou Chenuo Biological Technology Co., Ltd.

[0043] The Centella asiatica extract is from Guangzhou Mingli Biological Technology Co., Ltd.

[0044] The remaining raw materials are all conventional commercially available cosmetic grade.

[0045] In addition, the terms "first", "second", "third", etc. are used only for descriptive purposes and are not to be construed as indicating or implying relative importance or an ordered ranking of the indicated technical features. Thus, features defined with "first", "second" or "third" can include at least one of the features, either explicitly or implicitly. In the description of the present application, the meaning of "a plurality of" is at least two, for example, two, three, etc., unless otherwise explicitly and specifically limited.

[0046] The present application provides a composition containing pearl extract, which comprises pearl extract, leafy artemisia extract and artemisia capillaris extract.

[0047] The mass ratio of the pearl extract, leafy artemisia extract and artemisia capillaris extract is (0.1-0.6):(0.1-0.5):(0.1-1).

[0048] The pearl extract has excellent moisturizing effect, can absorb moisture in the air and lock in moisture, increase the moisture content of the skin, reduce transdermal water loss, keep the skin in a moist state, has excellent tissue repair effect, reduces the occurrence of sensitive phenomenon, can promote epidermal growth factor expression and promote cell proliferation, can remove the aging keratinocytes on the surface of the skin, promote the metabolism of the skin, repair damaged skin, can effectively scavenge free radicals, slow down the aging process of cells, thereby improving the elasticity, tightness and skin color uniformity of the skin, reducing the damage of oxidative stress to the skin, relieving the discomfort of the skin caused by external stimuli (such as ultraviolet rays, pollutants, etc.), reducing the tightness and swelling of the skin.

[0049] The leafy artemisia extract has excellent soothing and anti-allergic effect, can effectively relieve the discomfort such as redness and itching caused by external stimuli, also has anti-inflammatory and antibacterial effects, effectively relieves the symptoms such as redness and pain of the skin caused by inflammation, effectively inhibits the growth and reproduction of bacteria, thereby reducing the skin problems caused by bacterial infection, at the same time, the leafy artemisia extract has excellent moisturizing effect, can effectively lock in moisture, improve the problems such as dryness and water deficiency of the skin, and the leafy artemisia extract also has excellent antioxidant effect, can eliminate free radicals and reduce the damage of oxidative stress to the skin.

[0050] The artemisia capillaris extract has excellent anti-inflammatory soothing effect, can inhibit immune cell activation and reduce the effect of pro-inflammatory factor production, thereby effectively inhibiting skin inflammation, relieving skin redness, itching, inflammation and the like, inhibiting histamine release and stabilizing mast cell membrane, reducing skin allergy problems, can effectively remove free radicals, slow down the skin aging process, and the artemisia capillaris extract can also nourish the skin, promote skin nutrient absorption and moisture, enhance skin elasticity and toughness, thereby achieving the effect of moisturizing.

[0051] The application creatively combines the above raw materials, and under the synergistic effect of the pearl extract, the artemisia capillaris leaf extract and the artemisia capillaris extract, a composition with excellent moisturizing, antioxidant and soothing effects is obtained. The composition can absorb moisture in the air and lock the moisture, reduce transdermal water loss, effectively nourish the skin, promote skin nutrient absorption and moisture, effectively repair the skin barrier, relieve the discomfort of the skin caused by external stimulation (such as ultraviolet rays, pollutants and the like), reduce the tightness and swelling of the skin, effectively reduce the swelling, pain and the like caused by inflammation, effectively inhibit the growth and reproduction of bacteria, thereby reducing the skin problems caused by bacterial infection, relieving skin redness, itching, inflammation and the like, inhibiting histamine release and stabilizing mast cell membrane, reducing skin allergy problems, effectively removing free radicals, reducing the damage of oxidative stress to the skin, and having a wide application prospect.

[0052] In one embodiment, the preparation method of the pearl extract comprises the following steps:

[0053] (1) adding pearl powder into water, ultrasonic treatment, then adding papain, neutral protease and β-glucanase, and enzyme hydrolysis to obtain an enzyme hydrolysate;

[0054] (2) sequentially subjecting the enzyme hydrolysate to ultrafiltration with an ultrafiltration membrane with a molecular weight cut-off of 1000 Da and an ultrafiltration membrane with a molecular weight cut-off of 10000 Da to obtain the pearl extract.

[0055] The pearl powder is subjected to ultrasonic treatment, and then enzyme hydrolysis, and sequentially subjected to ultrafiltration with an ultrafiltration membrane with a molecular weight cut-off of 1000 Da and an ultrafiltration membrane with a molecular weight cut-off of 10000 Da, thereby obtaining the pearl extract with excellent moisturizing, soothing and antioxidant effects, and the pearl extract retains small molecular weight, which is beneficial to skin absorption.

[0056] It should be noted that the pearl powder in the application is derived from the triangle shell, which is crushed to 200-500 mesh from the pearl of the triangle shell with a diameter of 5-7 mm.

[0057] In one of the embodiments, the power of the ultrasonic treatment is 500-800 W, and the time of the ultrasonic treatment is 25-40 min.

[0058] In one of the embodiments, the mass ratio of the pearl powder, water, papain, neutral protease, and beta-glucanase is 1:(4-10):(0.01-0.03):(0.005-0.02):(0.005-0.02).

[0059] In one of the embodiments, the temperature of the enzymolysis is 48-55℃, and the time of the enzymolysis is 1-5 h.

[0060] In one of the embodiments, the preparation method of the Artemisia capillaris extract comprises the following steps:

[0061] (1) performing supercritical extraction on the Artemisia capillaris powder to obtain an extraction liquid;

[0062] (2) adding the extraction liquid, an enzymolysis promoter, and a compound enzyme into water, performing enzymolysis to obtain an enzymolysis liquid;

[0063] (3) adjusting the pH of the enzymolysis liquid to 6.2-6.8, passing the enzymolysis liquid through a macroporous adsorption resin at a flow rate of 1-2 BV / h, and eluting at a speed of 0.5-2 BV / h, first washing 1-2 BV of water, then washing 1-2 BV of 60-85 wt% ethanol solution, collecting the ethanol solution eluate, and drying to obtain the Artemisia capillaris extract.

[0064] In one of the embodiments, the supercritical extraction uses CO2 as an extractant, 75-95 wt% ethanol as a entrainer, the CO2 flow rate is 60-120 mL / min, the extraction temperature is 38-45℃, the extraction pressure is 28-32 Mpa, and the extraction time is 50-80 min.

[0065] The present application creatively performs supercritical extraction, enzymolysis treatment, and macroporous adsorption resin treatment on the Artemisia capillaris powder, can sufficiently extract the components with moisturizing, soothing, and antioxidant effects in the Artemisia capillaris, and obtains the Artemisia capillaris extract with excellent moisturizing, soothing, and antioxidant effects. The Artemisia capillaris can inhibit the activation of immune cells and reduce the production of pro-inflammatory factors, thereby effectively inhibiting skin inflammation, relieving skin redness, itching, inflammation, and the like, inhibiting histamine release and stabilizing mast cell membranes, and reducing skin allergy problems. The Artemisia capillaris extract can effectively scavenge free radicals, slow down the skin aging process, and also can nourish the skin, promote the nutrient absorption and moisturizing of the skin, and enhance the elasticity and toughness of the skin, thereby achieving the moisturizing effect.

[0066] The inventor of the present application has found that the effects of Artemisia capillaris extracts prepared by different extraction methods are different, and the method of the present application can more effectively promote the dissolution, extraction and enrichment of ingredients for moisturizing, soothing and antioxidant effects, and if the preparation method of Artemisia capillaris extract is different from the present application, the moisturizing, soothing and antioxidant effects will be reduced.

[0067] In one embodiment, the enzyme digestion promoter includes at least one of sodium carbonate and sodium bicarbonate, wherein the enzyme digestion promoter can reduce the extraction conditions, increase the permeability and osmotic pressure, promote the dissolution of active ingredients, and improve the enzyme digestion efficiency.

[0068] It should be noted that the Artemisia capillaris powder is obtained by crushing Artemisia capillaris powder, and the particle size is 50-200 mesh.

[0069] In one embodiment, the complex enzyme includes cellulase, xylanase and beta-glucanase, and the mass ratio of the cellulase, xylanase and beta-glucanase is 1:(0.4-0.8):(0.4-0.8).

[0070] In one embodiment, the mass ratio of the extraction liquid, enzyme digestion promoter, complex enzyme and water is 1:(0.005-0.02):(0.02-0.04):(4-10).

[0071] In one embodiment, the temperature of enzyme digestion is 48-55℃, and the time of enzyme digestion is 1-5h.

[0072] In one embodiment, the mass ratio of the total mass of the pearl extract and the Echinacea angustifolia leaf extract to the mass of the Artemisia capillaris extract is 1:(0.5-3). Especially when the amount of each raw material is controlled within this range, the compatibility of each raw material is better, the synergistic effect is further improved, each component can play a more full role, and the soothing and antioxidant effects are further improved.

[0073] The present application also provides a pearl cream, which comprises the following components in mass parts: 0.3-2.1 parts of a composition containing pearl extract, 0.05-0.2 parts of a tremella fruiting body extract, 0.05-0.2 parts of glycerol glucoside, 0.2-0.6 parts of tocopheryl acetate, 0.2-0.6 parts of betaine, 0.2-0.6 parts of a thickening agent, 0.5-2 parts of methylpropanediol, 2-3.5 parts of an emulsifier, 1-4 parts of butanediol, 4-8 parts of glycerol, 4-10 parts of an emollient, and 70-85 parts of water.

[0074] The composition containing pearl extract is the composition containing pearl extract described above.

[0075] In one embodiment, the thickening agent includes at least one of xanthan gum, hydrolyzed micrococcus gum, hydroxyethyl cellulose, carbomer, carrageenan, guar gum, locust bean gum, carboxymethyl cellulose, and sodium polyacrylate.

[0076] In one embodiment, the emollient includes at least one of squalane, shea butter, ethylhexyl palmitate, caprylic / capric triglyceride, jojoba oil, isononyl isononanoate, isododecane, isohexadecane, octyldodecanol, dicaprylyl carbonate, and macadamia integrifolia oil.

[0077] In one embodiment, the humectant includes squalane, shea butter, ethylhexyl palmitate, caprylic / capric triglyceride, and jojoba oil; and the mass ratio of the squalane, shea butter, ethylhexyl palmitate, caprylic / capric triglyceride, and jojoba oil is (1-3):(1-2):(1-2):(0.5-2):(0.5-1). In particular, when the humectant is composed of squalane, shea butter, ethylhexyl palmitate, caprylic / capric triglyceride, and jojoba oil in the mass ratio, the humectant can have excellent skin nourishing effect, promote the formation of an emulsified isolation layer on the skin surface, prevent water evaporation from the skin, increase the defense ability of the skin, and promote the penetration of active ingredients and the absorption of the skin.

[0078] In one embodiment, the emulsifier includes at least one of glyceryl stearate, PEG-100 stearate, sorbitan olivate, PEG-30 soya sterol, polyglyceryl-10 stearate, polyglyceryl-10 myristate, polyglyceryl-5 trioleate, PEG-40 hydrogenated castor oil, PEG-60 hydrogenated castor oil, PPG-13-decyltetradecin polyether-24, nonylphenol polyether-12, stearyl alcohol polyether-2, stearyl alcohol polyether-21, and hydrogenated lecithin.

[0079] In one embodiment, the emulsifier includes glyceryl stearate, PEG-100 stearate, and sorbitan olivate; and the mass ratio of the glyceryl stearate, PEG-100 stearate, and sorbitan olivate is (1-1.8):(0.8-1.2):(0.2-0.5). In particular, when the emulsifier is composed of glyceryl stearate, PEG-100 stearate, and sorbitan olivate in the mass ratio, the emulsifier can promote the formation of a stable cream, improve the stability of the system, avoid delamination and demulsification, promote the formation of a moisturizing film on the skin surface, improve the moisturizing effect, enhance the skin barrier, and improve the resistance of the skin to external stimuli.

[0080] In one embodiment, the pearl cream further includes a cosmetically acceptable auxiliary.

[0081] In one of the embodiments, the cosmetically acceptable adjuvant includes, but is not limited to, at least one of a humectant, an antioxidant, a preservative, a fragrance, a colorant, a pH adjusting agent.

[0082] In one of the embodiments, the suitable humectant includes, but is not limited to, at least one of sodium hyaluronate, hydroxyethyl urea, polyethylene glycol, xylitol, maltose, sodium polyglutamate, erythritol, sorbitol, mannitol, glucose, lactose, betaine, inositol, trehalose, hydrogenated starch hydrolysate.

[0083] In one of the embodiments, the suitable antioxidant includes, but is not limited to, ascorbic acid and its derivatives, resveratrol, pentaerythrityl tetra-di-t-butyl hydroxyhydrocinnamate, arbutin, sodium metabisulfite, and combinations thereof.

[0084] In one of the embodiments, the suitable colorant includes, but is not limited to, white, black, yellow, blue, green, pink, red, orange, purple, indigo, brown, and the like, and combinations thereof.

[0085] In one of the embodiments, the suitable pH adjusting agent includes, but is not limited to, arginine, tromethamine, aminomethyl propanol, tetrahydroxypropyl ethylenediamine, triethanolamine, citric acid, sodium citrate, sodium hydroxide, potassium hydroxide, and combinations thereof.

[0086] In one of the embodiments, the suitable preservative includes, but is not limited to, p-hydroxyacetophenone, 1,2-hexanediol, 1,2-pentanediol, isopentyl alcohol, ethylhexylglycerin, caprylyl glycol, chlorphenesin, hydroxybenzoic acid ester, potassium sorbate, sodium benzoate, phenoxyethanol, caprylhydroxaminc acid, glyceryl caprylate, cetylpyridinium chloride, cetalkonium chloride, and combinations thereof.

[0087] The present application also provides a preparation method of the pearl cream, comprising the following steps:

[0088] Mixing methylpropanediol, butanediol, glycerol, thickening agent, and water uniformly, homogenizing to obtain an aqueous phase;

[0089] Heating the emulsifier and emollient to 80-85°C, mixing uniformly to obtain an oil phase;

[0090] Adding the oil phase to the aqueous phase, stirring uniformly at 80-85°C, homogenizing, cooling to 40-45°C, adding the remaining materials, stirring uniformly, homogenizing to obtain the pearl cream.

[0091] The following examples are provided to facilitate an understanding of the present application. The examples are provided without any intention of limiting the scope of the claims.

[0092] Example 1

[0093] A pearl cream, comprising the following components in parts by mass: 1.2 parts of a pearl extract-containing composition, 0.1 parts of a tremella fruiting body extract, 0.1 parts of glyceryl glucoside, 0.5 parts of tocopheryl acetate, 0.5 parts of betaine, 0.4 parts of xanthan gum, 1 part of methylpropanediol, 3 parts of an emulsifier, 3 parts of butanediol, 6 parts of glycerol, 7 parts of an emollient, and water to 100 parts.

[0094] The pearl extract-containing composition comprises pearl extract, chamaebatia australis leaf extract, and artemisia capillaris extract; the mass ratio of the pearl extract, chamaebatia australis leaf extract, and artemisia capillaris extract is 0.2:0.2:0.8.

[0095] The mass ratio of the total mass of the pearl extract and chamaebatia australis leaf extract to the mass of the artemisia capillaris extract is 1:2.

[0096] The emollient comprises squalane, shea butter, ethylhexyl palmitate, caprylic / capric triglyceride, and jojoba oil, and the mass ratio of the squalane, shea butter, ethylhexyl palmitate, caprylic / capric triglyceride, and jojoba oil is 2.5:1.5:1.5:0.8:0.7.

[0097] The emulsifier comprises glyceryl stearate, PEG-100 stearate, and sorbitan olivate, and the mass ratio of the glyceryl stearate, PEG-100 stearate, and sorbitan olivate is 1.2:1:0.3.

[0098] The preparation method of the pearl extract comprises the following steps:

[0099] (1) 400-mesh pearl powder is added to water, and ultrasonic treatment is performed at 600 W for 30 min, and then papain, neutral protease, and β-glucanase are added, and enzymolysis is performed at 50℃ for 3 h to obtain an enzymolysis liquid; the mass ratio of the pearl powder, water, papain, neutral protease, and β-glucanase is 1:6:0.02:0.01:0.01.

[0100] (2) The enzymolysis liquid is sequentially subjected to ultrafiltration with an ultrafiltration membrane with a molecular weight cut-off of 1000 Da, and the cut-off liquid is collected, and the cut-off liquid is subjected to ultrafiltration with an ultrafiltration membrane with a molecular weight cut-off of 10000 Da, and the filtrate is collected, and the filtrate is the pearl extract.

[0101] The preparation method of the artemisia capillaris extract comprises the following steps:

[0102] (1) 200-mesh artemisia capillaris powder is subjected to supercritical extraction with an extraction kettle to obtain an extraction liquid; the supercritical extraction uses CO2 as an extractant, 90wt% ethanol as a entrainer, the CO2 flow rate is 100 mL / min, the extraction temperature is 42℃, the extraction pressure is 30 Mpa, and the extraction time is 60 min.

[0103] (2) adding the extract, sodium bicarbonate and complex enzyme into water, and carrying out enzymolysis at 50℃ for 3h to obtain an enzymolysis solution; the mass ratio of the extract, sodium bicarbonate, complex enzyme and water is 1:0.01:0.03:6; the complex enzyme comprises cellulase, xylanase and beta-glucanase, and the mass ratio of the cellulase, xylanase and beta-glucanase is 1:0.5:0.5.

[0104] (3) adjusting the pH of the enzymolysis solution to 6.5, and passing the solution through D101 macroporous adsorption resin at a flow rate of 1.5BV / h, and eluting at a flow rate of 1BV / h, and then washing with water at 1.5BV and 80wt% ethanol solution at 1.5BV, and collecting the ethanol solution eluent, and drying to obtain the Artemisia capillaris extract.

[0105] The preparation method of the pearl cream comprises the following steps:

[0106] methylpropanediol, butanediol, glycerol, xanthan gum and water are uniformly mixed at 82℃ and 30rpm, and homogenized at 1800rpm for 4min to obtain an aqueous phase;

[0107] the emulsifier and the emollient are heated to 82℃, and uniformly mixed at 30rpm to obtain an oil phase;

[0108] the oil phase is added into the aqueous phase, and uniformly stirred at 30rpm at 82℃, and then homogenized at 1800rpm for 4min, and cooled to 42℃, and the remaining materials are added, and uniformly stirred at 30rpm, and homogenized at 1800rpm for 4min to obtain the pearl cream.

[0109] Example 2

[0110] Example 2 is different from Example 1 in that the use amounts of the pearl extract, the leaf extract of Chamaebatia australis and the Artemisia capillaris extract are different (the total use amount of the three is the same), and the others are the same.

[0111] A pearl cream comprises the following components in mass parts: 1.2 parts of a composition containing pearl extract, 0.1 part of a tremella fruiting body extract, 0.1 part of glycerol glucoside, 0.5 part of tocopheryl acetate, 0.5 part of betaine, 0.4 part of xanthan gum, 1 part of methylpropanediol, 3 parts of emulsifier, 3 parts of butanediol, 6 parts of glycerol, 7 parts of emollient, and water is added to 100 parts.

[0112] The composition containing pearl extract comprises pearl extract, leaf extract of Chamaebatia australis and Artemisia capillaris extract; and the mass ratio of the pearl extract, leaf extract of Chamaebatia australis and Artemisia capillaris extract is 0.15:0.15:0.9.

[0113] The total mass of the pearl extract and the pineleaf Echinacea extract and the mass of the Artemisia capillaris extract are in a ratio of 1:3.

[0114] Example 3

[0115] Example 3 is different from Example 1 in that the use amounts of the pearl extract, the pineleaf Echinacea extract, and the Artemisia capillaris extract are different (the total use amount of the three is the same), and the others are the same.

[0116] A pearl cream, comprising the following components in mass parts: 1.2 parts of a pearl extract-containing composition, 0.1 part of a tremella fruiting body extract, 0.1 part of glycerol glucoside, 0.5 part of tocopheryl acetate, 0.5 part of betaine, 0.4 part of xanthan gum, 1 part of methylpropanediol, 3 parts of an emulsifier, 3 parts of butanediol, 6 parts of glycerol, 7 parts of an emollient, and water to 100 parts.

[0117] The pearl extract-containing composition comprises a pearl extract, a pineleaf Echinacea extract, and an Artemisia capillaris extract; the mass ratio of the pearl extract, the pineleaf Echinacea extract, and the Artemisia capillaris extract is 0.4:0.4:0.4.

[0118] The total mass of the pearl extract and the pineleaf Echinacea extract and the mass of the Artemisia capillaris extract are in a ratio of 1:0.5.

[0119] Example 4

[0120] Example 4 is different from Example 1 in that the mass ratio of the pearl extract, the pineleaf Echinacea extract, and the Artemisia capillaris extract is different (the total use amount of the three is the same), and the others are the same.

[0121] A pearl cream, comprising the following components in mass parts: 1.2 parts of a pearl extract-containing composition, 0.1 part of a tremella fruiting body extract, 0.1 part of glycerol glucoside, 0.5 part of tocopheryl acetate, 0.5 part of betaine, 0.4 part of xanthan gum, 1 part of methylpropanediol, 3 parts of an emulsifier, 3 parts of butanediol, 6 parts of glycerol, 7 parts of an emollient, and water to 100 parts.

[0122] The pearl extract-containing composition comprises a pearl extract, a pineleaf Echinacea extract, and an Artemisia capillaris extract; the mass ratio of the pearl extract, the pineleaf Echinacea extract, and the Artemisia capillaris extract is 0.1:0.1:1.

[0123] The total mass of the pearl extract and the pineleaf Echinacea extract and the mass of the Artemisia capillaris extract are in a ratio of 1:5.

[0124] Example 5

[0125] Example 5 differs from Example 1 in that the amounts of pearl extract, leaf of Arabidopsis leaf extract, and extract of Artemisia capillaris are different (the total amount of the three is the same), and the others are the same.

[0126] A pearl cream includes the following components in mass parts: 1.2 parts of a composition containing pearl extract, 0.1 parts of an extract of Tremella fuciformis, 0.1 parts of glycerol glucoside, 0.5 parts of tocopheryl acetate, 0.5 parts of betaine, 0.4 parts of xanthan gum, 1 part of methylpropanediol, 3 parts of emulsifier, 3 parts of butanediol, 6 parts of glycerol, 7 parts of emollient, and water is added to 100 parts.

[0127] The composition containing pearl extract includes pearl extract, leaf of Arabidopsis leaf extract, and extract of Artemisia capillaris; the mass ratio of the pearl extract, leaf of Arabidopsis leaf extract, and extract of Artemisia capillaris is 0.6:0.5:0.1.

[0128] The mass ratio of the total mass of the pearl extract and leaf of Arabidopsis leaf extract to the mass of the extract of Artemisia capillaris is 11:1.

[0129] Example 6

[0130] Example 6 differs from Example 1 in that the amounts of other raw materials are different (the mass ratio of pearl extract, leaf of Arabidopsis leaf extract, and extract of Artemisia capillaris is the same), and the others are the same.

[0131] A pearl cream includes the following components in mass parts: 1.2 parts of a composition containing pearl extract, 0.05 parts of an extract of Tremella fuciformis, 0.2 parts of glycerol glucoside, 0.2 parts of tocopheryl acetate, 0.6 parts of betaine, 0.2 parts of xanthan gum, 2 parts of methylpropanediol, 2 parts of emulsifier, 4 parts of butanediol, 4 parts of glycerol, 10 parts of emollient, and water is added to 100 parts.

[0132] The emollient includes squalane, shea butter, ethylhexyl palmitate, caprylic / capric triglyceride, and jojoba oil; the mass ratio of the squalane, shea butter, ethylhexyl palmitate, caprylic / capric triglyceride, and jojoba oil is 3:2:2:2:1.

[0133] The emulsifier includes glyceryl stearate, PEG-100 stearate, and sorbitan olivate; the mass ratio of the glyceryl stearate, PEG-100 stearate, and sorbitan olivate is 1:0.8:0.2.

[0134] Example 7

[0135] Example 7 differs from Example 1 in that the amounts of other raw materials are different (the mass ratio of pearl extract, leaf of Arabidopsis leaf extract, and extract of Artemisia capillaris is the same), and the others are the same.

[0136] A pearl cream, comprising the following components in mass parts: 1.2 parts of a composition containing pearl extract, 0.2 parts of tremella fruiting body extract, 0.05 parts of glyceryl glucoside, 0.6 parts of tocopheryl acetate, 0.2 parts of betaine, 0.6 parts of xanthan gum, 0.5 parts of methylpropanediol, 3.5 parts of emulsifier, 1 part of butanediol, 8 parts of glycerol, 4 parts of emollient, and water to 100 parts.

[0137] The wetting agent comprises squalane, avocado butter, ethylhexyl palmitate, caprylic / capric triglyceride, and jojoba oil; the mass ratio of the squalane, avocado butter, ethylhexyl palmitate, caprylic / capric triglyceride, and jojoba oil is 1:1:1:0.5:0.5.

[0138] The emulsifier comprises glyceryl stearate, PEG-100 stearate, and sorbitan olivate; the mass ratio of the glyceryl stearate, PEG-100 stearate, and sorbitan olivate is 1.8:1.2:0.5.

[0139] Example 8

[0140] Example 8 differs from Example 1 in that the preparation parameters of the pearl extract and the artemisia extract are different, and the others are the same.

[0141] The preparation method of the pearl extract comprises the following steps:

[0142] (1) 400-mesh pearl powder is added to water, treated with ultrasonic waves at 600 W for 30 min, and then papain, neutral protease, and β-glucanase are added, and enzymolysis is performed at 50℃ for 3 h to obtain an enzymolysis liquid; the mass ratio of the pearl powder, water, papain, neutral protease, and β-glucanase is 1:4:0.01:0.005:0.02.

[0143] (2) The enzymolysis liquid is sequentially subjected to ultrafiltration with an ultrafiltration membrane with a molecular weight cut-off of 1000 Da, and the cut-off liquid is collected; the cut-off liquid is subjected to ultrafiltration with an ultrafiltration membrane with a molecular weight cut-off of 10000 Da, and the filtrate is collected, which is the pearl extract.

[0144] The preparation method of the artemisia extract comprises the following steps:

[0145] (1) 200-mesh artemisia powder is subjected to supercritical extraction with an extraction kettle to obtain an extraction liquid; the supercritical extraction uses CO2 as an extractant, 90wt% ethanol as a entrainer, the CO2 flow rate is 100 mL / min, the extraction temperature is 42℃, the extraction pressure is 30 Mpa, and the extraction time is 60 min.

[0146] (2) adding the extract, sodium bicarbonate and complex enzyme into water, and carrying out enzymolysis at 50 DEG C for 3h to obtain an enzymolysis solution; the mass ratio of the extract, sodium bicarbonate, complex enzyme and water is 1:0.005:0.04:10; the complex enzyme comprises cellulase, xylanase and beta-glucanase, and the mass ratio of the cellulase, xylanase and beta-glucanase is 1:0.8:0.4.

[0147] (3) adjusting the pH of the enzymolysis solution to 6.5, and passing the solution through D101 macroporous adsorption resin at a flow rate of 1.5 BV / h, and then washing the resin with water at a flow rate of 1.5 BV / h, and then washing the resin with 80wt% ethanol solution at a flow rate of 1.5 BV / h, collecting the ethanol solution eluate, and drying to obtain the artemisia capillaris extract.

[0148] Example 9

[0149] Example 9 is different from example 1 in that the preparation parameters of the pearl extract and the artemisia capillaris extract are different, and other aspects are the same.

[0150] The preparation method of the pearl extract comprises the following steps:

[0151] (1) adding 400-mesh pearl powder into water, and carrying out ultrasonic treatment at 600W for 30min, and then adding papain, neutral protease and beta-glucanase, and carrying out enzymolysis at 50 DEG C for 3h to obtain an enzymolysis solution; the mass ratio of the pearl powder, water, papain, neutral protease and beta-glucanase is 1:10:0.03:0.02:0.005.

[0152] (2) sequentially ultrafiltering the enzymolysis solution with an ultrafiltration membrane with a molecular weight cut-off of 1000Da to collect the cut-off liquid, and then ultrafiltering the cut-off liquid with an ultrafiltration membrane with a molecular weight cut-off of 10000Da to collect the filtrate, and the filtrate is the pearl extract.

[0153] The preparation method of the artemisia capillaris extract comprises the following steps:

[0154] (1) carrying out supercritical extraction on 200-mesh artemisia capillaris powder with an extraction kettle to obtain an extract; the supercritical extraction uses CO2 as an extractant, 90wt% ethanol as a entraining agent, the CO2 flow rate is 100mL / min, the extraction temperature is 42 DEG C, the extraction pressure is 30Mpa, and the extraction time is 60min.

[0155] (2) adding the extract, sodium bicarbonate and complex enzyme into water, and carrying out enzymolysis at 50 DEG C for 3h to obtain an enzymolysis solution; the mass ratio of the extract, sodium bicarbonate, complex enzyme and water is 1:0.02:0.02:4; the complex enzyme comprises cellulase, xylanase and beta-glucanase, and the mass ratio of the cellulase, xylanase and beta-glucanase is 1:0.4:0.8.

[0156] (3) The enzymatic hydrolysate was adjusted to pH 6.5, and passed through D101 macroporous adsorption resin at a flow rate of 1.5 BV / h, and eluted at a rate of 1 BV / h, first washed with water at 1.5 BV, then washed with 80 wt% ethanol solution at 1.5 BV, and the ethanol eluate was collected and dried to obtain the extract of Artemisia capillaris.

[0157] Comparative Example 1

[0158] Comparative Example 1 differs from Example 1 in that Comparative Example 1 is a single pearl extract (replacing the composition, total amount unchanged), and the others are the same.

[0159] A pearl cream, comprising the following components in mass parts: 1.2 parts of pearl extract, 0.1 part of tremella fruit body extract, 0.1 part of glycerol glucoside, 0.5 part of tocopheryl acetate, 0.5 part of betaine, 0.4 part of xanthan gum, 1 part of methylpropanediol, 3 parts of emulsifier, 3 parts of butanediol, 6 parts of glycerol, 7 parts of emollient, and water to 100 parts.

[0160] Comparative Example 2

[0161] Comparative Example 2 differs from Example 1 in that Comparative Example 2 is a single extract of Echinacea angustifolia leaf (replacing the composition, total amount unchanged), and the others are the same.

[0162] A pearl cream, comprising the following components in mass parts: 1.2 parts of Echinacea angustifolia leaf extract, 0.1 part of tremella fruit body extract, 0.1 part of glycerol glucoside, 0.5 part of tocopheryl acetate, 0.5 part of betaine, 0.4 part of xanthan gum, 1 part of methylpropanediol, 3 parts of emulsifier, 3 parts of butanediol, 6 parts of glycerol, 7 parts of emollient, and water to 100 parts.

[0163] Comparative Example 3

[0164] Comparative Example 3 differs from Example 1 in that Comparative Example 3 is a single extract of Artemisia capillaris (replacing the composition, total amount unchanged), and the others are the same.

[0165] A pearl cream, comprising the following components in mass parts: 1.2 parts of Artemisia capillaris extract, 0.1 part of tremella fruit body extract, 0.1 part of glycerol glucoside, 0.5 part of tocopheryl acetate, 0.5 part of betaine, 0.4 part of xanthan gum, 1 part of methylpropanediol, 3 parts of emulsifier, 3 parts of butanediol, 6 parts of glycerol, 7 parts of emollient, and water to 100 parts.

[0166] Comparative Example 4

[0167] Comparative Example 4 differs from Example 1 in that Comparative Example 4 does not contain pearl extract (total amount of the three unchanged), and the others are the same.

[0168] A pearl cream, comprising the following components in mass parts: 1.2 parts of a composition, 0.1 part of tremella fruiting body extract, 0.1 part of glycerol glucoside, 0.5 part of tocopheryl acetate, 0.5 part of betaine, 0.4 part of xanthan gum, 1 part of methyl propylene glycol, 3 parts of emulsifier, 3 parts of butylene glycol, 6 parts of glycerol, 7 parts of emollient, and water to 100 parts.

[0169] The composition comprises the leaf extract of Pinus sylvestris L. and the extract of Artemisia capillaris Thunb.; the mass ratio of the leaf extract of Pinus sylvestris L. to the extract of Artemisia capillaris Thunb. is 0.2:0.8.

[0170] Comparative Example 5

[0171] Comparative Example 5 differs from Example 1 in that Comparative Example 5 does not contain the leaf extract of Pinus sylvestris L. (the total amount of the three is unchanged), and the others are the same.

[0172] A pearl cream, comprising the following components in mass parts: 1.2 parts of a composition containing pearl extract, 0.1 part of tremella fruiting body extract, 0.1 part of glycerol glucoside, 0.5 part of tocopheryl acetate, 0.5 part of betaine, 0.4 part of xanthan gum, 1 part of methyl propylene glycol, 3 parts of emulsifier, 3 parts of butylene glycol, 6 parts of glycerol, 7 parts of emollient, and water to 100 parts.

[0173] The composition containing pearl extract comprises pearl extract and the extract of Artemisia capillaris Thunb.; the mass ratio of the pearl extract to the extract of Artemisia capillaris Thunb. is 0.2:0.8.

[0174] Comparative Example 6

[0175] Comparative Example 6 differs from Example 1 in that Comparative Example 6 does not contain the extract of Artemisia capillaris Thunb. (the total amount of the three is unchanged), and the others are the same.

[0176] A pearl cream, comprising the following components in mass parts: 1.2 parts of a composition containing pearl extract, 0.1 part of tremella fruiting body extract, 0.1 part of glycerol glucoside, 0.5 part of tocopheryl acetate, 0.5 part of betaine, 0.4 part of xanthan gum, 1 part of methyl propylene glycol, 3 parts of emulsifier, 3 parts of butylene glycol, 6 parts of glycerol, 7 parts of emollient, and water to 100 parts.

[0177] The composition containing pearl extract comprises pearl extract and the leaf extract of Pinus sylvestris L.; the mass ratio of the pearl extract to the leaf extract of Pinus sylvestris L. is 0.2:0.2.

[0178] Comparative Example 7

[0179] Comparative Example 7 differs from Example 1 in that the mass ratio of the pearl extract, the leaf extract of Pinus sylvestris L., and the extract of Artemisia capillaris Thunb. is not within the scope of the present application (the total amount is unchanged), and the others are the same.

[0180] A pearl cream, comprising the following components in mass parts: 1.2 parts of a pearl extract-containing composition, 0.1 part of a tremella fruiting body extract, 0.1 part of glycerol glucoside, 0.5 part of tocopheryl acetate, 0.5 part of betaine, 0.4 part of xanthan gum, 1 part of methylpropanediol, 3 parts of an emulsifier, 3 parts of butanediol, 6 parts of glycerol, 7 parts of an emollient, and water to 100 parts.

[0181] The pearl extract-containing composition comprises pearl extract, leafy speedwell extract, and artemisia capillaris extract; the mass ratio of the pearl extract, leafy speedwell extract, and artemisia capillaris extract is 0.025:0.025:1.15.

[0182] Comparative Example 8

[0183] Comparative Example 8 differs from Example 1 in that the mass ratio of the pearl extract, leafy speedwell extract, and artemisia capillaris extract is not within the range of the present application (the total amount is unchanged), and the other components are the same.

[0184] A pearl cream, comprising the following components in mass parts: 1.2 parts of a pearl extract-containing composition, 0.1 part of a tremella fruiting body extract, 0.1 part of glycerol glucoside, 0.5 part of tocopheryl acetate, 0.5 part of betaine, 0.4 part of xanthan gum, 1 part of methylpropanediol, 3 parts of an emulsifier, 3 parts of butanediol, 6 parts of glycerol, 7 parts of an emollient, and water to 100 parts.

[0185] The pearl extract-containing composition comprises pearl extract, leafy speedwell extract, and artemisia capillaris extract; the mass ratio of the pearl extract, leafy speedwell extract, and artemisia capillaris extract is 0.05:1.1:0.05.

[0186] Comparative Example 9

[0187] Comparative Example 9 differs from Example 1 in that Comparative Example 9 uses an equal amount of purslane extract to replace the leafy speedwell extract, and the other components are the same.

[0188] A pearl cream, comprising the following components in mass parts: 1.2 parts of a pearl extract-containing composition, 0.1 part of a tremella fruiting body extract, 0.1 part of glycerol glucoside, 0.5 part of tocopheryl acetate, 0.5 part of betaine, 0.4 part of xanthan gum, 1 part of methylpropanediol, 3 parts of an emulsifier, 3 parts of butanediol, 6 parts of glycerol, 7 parts of an emollient, and water to 100 parts.

[0189] The pearl extract-containing composition comprises pearl extract, purslane extract, and artemisia capillaris extract; the mass ratio of the pearl extract, purslane extract, and artemisia capillaris extract is 0.2:0.2:0.8.

[0190] Comparative Example 10

[0191] Comparative Example 10 differs from Example 1 in that Comparative Example 10 uses an equal amount of Centella asiatica extract to replace the Artemisia capillaris extract, and the others are the same.

[0192] A pearl cream, comprising the following components in mass parts: 1.2 parts of a composition containing pearl extract, 0.1 part of tremella fruit body extract, 0.1 part of glycerol glucoside, 0.5 part of tocopheryl acetate, 0.5 part of betaine, 0.4 part of xanthan gum, 1 part of methylpropanediol, 3 parts of emulsifier, 3 parts of butanediol, 6 parts of glycerol, 7 parts of emollient, and water to 100 parts.

[0193] The composition containing pearl extract comprises pearl extract, leaf extract of Chamaebatia millefolia and Centella asiatica extract; the mass ratio of the pearl extract, the leaf extract of Chamaebatia millefolia and the Centella asiatica extract is 0.2:0.2:0.8.

[0194] Comparative Example 11

[0195] Comparative Example 11 differs from Example 1 in that the preparation method of Artemisia capillaris extract is different, and the others are the same.

[0196] The preparation method of Artemisia capillaris extract in the present comparative example comprises the following steps:

[0197] (1) 200 parts of Artemisia capillaris powder, sodium bicarbonate and compound enzyme are added to water, and enzymolysis is carried out at 50°C for 3h to obtain an enzymolysis liquid; the mass ratio of the Artemisia capillaris powder, sodium bicarbonate, compound enzyme and water is 1:0.01:0.03:6; the compound enzyme comprises cellulase, xylanase and β-glucanase, and the mass ratio of the cellulase, xylanase and β-glucanase is 1:0.5:0.5.

[0198] (3) The pH of the enzymolysis liquid is adjusted to 6.5, and the enzymolysis liquid is passed through D101 macroporous adsorption resin at a flow rate of 1.5BV / h, and elution is carried out at a speed of 1BV / h, and then 1.5BV of water is used for washing, and then 1.5BV of 80wt% ethanol solution is used for washing, and the ethanol eluate is collected and dried to obtain Artemisia capillaris extract.

[0199] Comparative Example 12

[0200] Comparative Example 12 differs from Example 1 in that the preparation method of Artemisia capillaris extract is different, and the others are the same.

[0201] The preparation method of Artemisia capillaris extract in the present comparative example comprises the following steps:

[0202] (1) The 200 mesh Artemisia capillaris powder is subjected to supercritical extraction in an extraction kettle to obtain an extraction liquid; the supercritical extraction uses CO2 as an extractant, 90wt% ethanol as an entraining agent, the CO2 flow rate is 100mL / min, the extraction temperature is 42℃, the extraction pressure is 30Mpa, and the extraction time is 60min.

[0203] (2) The extraction liquid and complex enzyme are added to water, and enzymolysis is carried out at 50℃ for 3h to obtain an enzymolysis liquid; the mass ratio of the extraction liquid, complex enzyme and water is 1:0.03:6; the complex enzyme comprises cellulase and pectinase, and the mass ratio of the cellulase and pectinase is 1:1.

[0204] (3) The enzymolysis liquid is adjusted to pH 6.5, and is passed through D101 macroporous adsorption resin at a flow rate of 1.5BV / h, and elution is carried out at a flow rate of 1BV / h, first using water at 1.5BV, and then using 80wt% ethanol solution at 1.5BV; the ethanol solution eluate is collected and dried to obtain an Artemisia capillaris extract.

[0205] Test Example

[0206] 1. The DPPH scavenging rate of the pearl cream of the test examples and the comparative examples is tested according to T / SHRH 006-2018 "Cosmetics - Free radical (DPPH) scavenging experiment method".

[0207] 2. The soothing effect of the pearl cream of the test examples and the comparative examples is evaluated by using a hyaluronidase inhibition rate experiment.

[0208] The test method is as follows:

[0209] 1. Experimental materials

[0210] 1.1 Instruments and materials

[0211] Main reagents: acetic acid buffer solution, hyaluronidase solution, sodium hyaluronate solution, sodium hydroxide solution, cetyltrimethylammonium bromide (CTAB) solution.

[0212] Main instruments: analytical balance, electric heating constant temperature incubator, microplate reader.

[0213] Table 1

[0214] Instrument name Instrument model Instrument manufacturer Analytical balance HX50002 Yongkang Yixian Electronic Technology Co., Ltd. Incubator with heating and constant temperature HWS-260H Ningbo Yanghui Instruments Co., Ltd. Microplate reader SpectraMAX i3 Molecular Devices

[0215] 1.2 Test sample: the pearl cream of the test examples and the comparative examples is diluted with water to a concentration of 25wt%.

[0216] 1.3 Preparation of reagents:

[0217] 1.3.1 Acetic acid buffer: Weigh 12.30 g of sodium acetate trihydrate. Dissolve in 800 mL of water, and adjust the pH to 5.6 with acetic acid (about 581 μL), and dilute to 1 L with water.

[0218] 1.3.2 Hyaluronidase solution: Weigh 10.00 mg of enzyme, dissolve in acetic acid buffer, and configure into 3000 U / mL stock solution, which is stored at -20°C. When long-term storage is needed, 0.01% BSA can be added for protection. Before use, dilute into 500 U / mL working solution with acetic acid buffer.

[0219] 1.3.3 Sodium hyaluronate solution: Weigh 200 mg of sodium hyaluronate solid, and swell in 20 g of tertiary water at 4°C overnight, to configure into 1% sodium hyaluronate solution, which can be stored at -20°C for long-term. Take 0.4 mL of 1% hyaluronate solution, and dilute into 0.04% hyaluronate aqueous solution with 9.6 mL of tertiary water.

[0220] 1.3.4 Sodium hydroxide solution: Weigh 2.00 g of sodium hydroxide, and dissolve in 100 mL of water.

[0221] 1.3.5 Cetyltrimethylammonium bromide (CTAB) solution: Weigh 0.25 g of CTAB, and dissolve in 100 mL of 2% sodium hydroxide solution.

[0222] 2. Test detection method

[0223] Table 2 Sample addition record

[0224]

[0225]

[0226] Hyaluronidase inhibition rate calculation formula:

[0227] Hyaluronidase inhibition rate (%) = {(D-C)-(B-A)} / (D-C) x 100%. In the formula, A is the OD value of the test solution of (hyaluronidase + sample + sodium hyaluronate). B is the OD value of the test blank of (acetic acid buffer + sample + acetic acid buffer). C is the OD value of the control solution of (hyaluronidase + deionized water + sodium hyaluronate). D is the OD value of the control blank of (acetic acid buffer + deionized water + acetic acid buffer).

[0228] Table 3

[0229]

[0230]

[0231] As can be seen from Table 3, the pearl cream according to the present application has excellent soothing and antioxidant effects.

[0232] As can be seen from Comparative Examples 1-5, by controlling the total mass of the pearl extract and the leaf extract of Chamaemelum nobile and the mass of the extract of Artemisia capillaris to be 1:(0.5-3), the compatibility of the raw materials is better, the synergistic effect is further improved, the components can play a more sufficient role, and the soothing and antioxidant effects are further improved.

[0233] As can be seen from Comparative Examples 1-5 and Comparative Examples 7-8, when the amounts of the pearl extract, the leaf extract of Chamaemelum nobile, and the extract of Artemisia capillaris are within the range of the present application, the ideal synergistic effect is achieved, and when the amounts of the raw materials deviate from the range of the present application, the soothing and antioxidant effects decrease.

[0234] As can be seen from Comparative Example 1 and Comparative Examples 1-6 and Comparative Examples 9-10, the pearl extract, the leaf extract of Chamaemelum nobile, and the extract of Artemisia capillaris according to the present application have a significant synergistic effect in soothing and antioxidant effects, and the lack of any one of them or the use of other raw materials to replace any one of them will result in a significant decrease in the soothing and antioxidant effects.

[0235] As can be seen from Comparative Example 1 and Comparative Examples 11-12, the soothing and antioxidant effects of the extract of Artemisia capillaris prepared by different methods are different, and the extract of Artemisia capillaris prepared by the preparation method of the present application significantly improves the soothing and antioxidant effects.

[0236] 3. Stability test: The examples and comparative examples were subjected to stability tests, the heat resistance test was to stand at 48℃ for two months, and the appearance, color, and odor were observed; the cold resistance test was to stand at -18℃ for two months, and the appearance, color, and odor were observed; the cold and heat cycle resistance test was to stand at 48℃, room temperature, and -18℃ for 24h each for 6 cycles, and the appearance, color, and odor were observed. The test results are shown in Table 4.

[0237] Table 4

[0238]

[0239]

[0240] As can be seen from Table 4, the pearl cream according to the present application has good stability.

[0241] Finally, it should be noted that the above examples are only used to illustrate the technical solutions of the present application and not to limit the protection scope of the present application. Although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application can be modified or replaced equivalently without departing from the essence and scope of the technical solutions of the present application.

Claims

1. A composition comprising pearl extract, characterized in that, The pearl extract, the artemisia scoparia leaf extract, and the artemisia capillaris extract; The mass ratio of the pearl extract, the artemisia scoparia leaf extract, and the artemisia capillaris extract is (0.1-0.6):(0.1-0.5):(0.1-1).

2. The pearl extract-containing composition according to claim 1, characterized by, The preparation method of the pearl extract comprises the following steps: (1) adding pearl powder into water, ultrasonic treatment, and then adding papain, neutral protease, and β-glucanase for enzymolysis to obtain an enzymolysis solution; (2) sequentially subjecting the enzymolysis solution to ultrafiltration with an ultrafiltration membrane with a molecular weight cut-off of 1000 Da and ultrafiltration with an ultrafiltration membrane with a molecular weight cut-off of 10000 Da to obtain the pearl extract.

3. The pearl extract-containing composition according to claim 2, characterized by, The ultrasonic treatment is performed at a power of 500-800 W for 25-40 min.

4. The pearl extract-containing composition according to claim 2, characterized by, The mass ratio of the pearl powder, water, papain, neutral protease, and β-glucanase is 1:(4-10):(0.01-0.03):(0.005-0.02):(0.005-0.02).

5. The pearl extract-containing composition according to claim 2, characterized by, The enzymolysis is performed at a temperature of 48-55 ℃ for 1-5 h.

6. The pearl extract-containing composition according to claim 1, characterized by, The preparation method of the artemisia capillaris extract comprises the following steps: (1) subjecting artemisia capillaris powder to supercritical extraction to obtain an extraction solution; (2) adding the extraction solution, an enzymolysis promoter, and a composite enzyme into water for enzymolysis to obtain an enzymolysis solution; (3) adjusting the pH of the enzymolysis solution to 6.2-6.8, passing the solution through a macroporous adsorption resin at a flow rate of 1-2 BV / h, and eluting at a speed of 0.5-2 BV / h, first washing 1-2 BV of water, then washing 1-2 BV of 60-85 wt% ethanol solution, collecting the ethanol solution eluate, and drying to obtain the artemisia capillaris extract.

7. The pearl extract-containing composition according to claim 6, characterized by, The supercritical extraction uses CO2 as an extractant and 75-95 wt% ethanol as a entrainer, the CO2 flow rate is 60-120 mL / min, the extraction temperature is 38-45 ℃, the extraction pressure is 28-32 Mpa, and the extraction time is 50-80 min.

8. The pearl extract-containing composition according to claim 6, characterized by, The enzymolysis promoter comprises at least one of sodium carbonate and sodium bicarbonate; and / or The composite enzyme comprises cellulase, xylanase, and β-glucanase, and the mass ratio of the cellulase, xylanase, and β-glucanase is 1:(0.4-0.8):(0.4-0.8).

9. The pearl extract-containing composition according to claim 6, characterized by, The mass ratio of the extraction solution, the enzymolysis promoter, the composite enzyme, and water is 1:(0.005-0.02):(0.02-0.04):(4-10).

10. The pearl extract-containing composition according to claim 6, characterized by, The enzymolysis is performed at a temperature of 48-55 ℃ for 1-5 h.

11. The pearl extract-containing composition according to claim 1, characterized by, The mass ratio of the total mass of the pearl extract and the artemisia scoparia leaf extract to the mass of the artemisia capillaris extract is 1:(0.5-3).

12. A pearl cream, characterized by, The composition comprises the following components in the following mass fractions: 0.3-2.1 parts of a composition containing a pearl extract, 0.05-0.2 parts of a tremella fruiting body extract, 0.05-0.2 parts of glycerol glucoside, 0.2-0.6 parts of tocopheryl acetate, 0.2-0.6 parts of betaine, 0.2-0.6 parts of a thickening agent, 0.5-2 parts of methylpropanediol, 2-3.5 parts of an emulsifier, 1-4 parts of butanediol, 4-8 parts of glycerol, 4-10 parts of an emollient, and 70-85 parts of water. The pearl extract-containing composition is the pearl extract-containing composition according to any one of claims 1-11.

13. The pearl cream according to claim 12, characterized in that, The thickening agent comprises at least one of xanthan gum, hydrolyzed micrococcus gum, hydroxyethyl cellulose, carbomer, carrageenan, guar gum, locust bean gum, carboxymethyl cellulose, and sodium polyacrylate.

14. The pearl cream according to claim 12, characterized in that, The emollient comprises at least one of squalane, shea butter, ethylhexyl palmitate, caprylic / capric triglyceride, jojoba oil, isononyl isononanoate, isododecane, isohexadecane, octyldodecanol, dicaprylyl carbonate, and macadamia integrifolia oil; and / or The emulsifier comprises at least one of glyceryl stearate, PEG-100 stearate, sorbitan olivate, PEG-30 soy sterol, polyglyceryl-10 stearate, polyglyceryl-10 myristate, polyglyceryl-5 trioleate, PEG-40 hydrogenated castor oil, PEG-60 hydrogenated castor oil, PPG-13-decyltetradecin polyol ether-24, nonylphenol polyol ether-12, stearyl alcohol polyol ether-2, stearyl alcohol polyol ether-21, and hydrogenated lecithin.

15. The method of producing the pearl cream according to any one of claims 12 to 14, characterized by, The method comprises the following steps: uniformly mixing methylpropanediol, butanediol, glycerol, a thickening agent, and water to obtain an aqueous phase; heating an emulsifier and an emollient to 80-85°C, uniformly mixing them to obtain an oil phase; adding the oil phase to the aqueous phase, uniformly stirring and homogenizing them at 80-85°C, cooling to 40-45°C, adding the remaining materials, uniformly stirring and homogenizing them to obtain the pearl cream.

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